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Haining Zhang, Dongyu Min, Mingyu Fu, Jing Tian, Qingwen Wang, Xinjiang An
OBJECTIVE: To explore the effects of astragalus (AST) , total flavone of astragalus (TFA), total saponins of astragalus (TSA) and astragalus polysaccharides (APS) on ischemia/reperfusion (40 min/60 min) injury in isolated guinea-pig heart. METHODS: Isolated guinea-pig hearts underwent ischemia, then followed by K-H perfusion (I/R group), AST (60 mg/L),AST (60 mg/L), TFA (60 mg/L), TSA (60 mg/L) and APS (60 mg/L) perfusion (n = 6 each).Isolated hearts without ischemia serve as control group (n = 6)...
September 2014: Zhonghua Xin Xue Guan Bing za Zhi
Björn Gerdle, Jesper Kristiansen, Britt Larsson, Bengt Saltin, Karen Søgaard, Gisela Sjøgaard
BACKGROUND: This study compares the levels of algesic substances between subjects with trapezius myalgia (TM) and healthy controls (CON) and explores the multivariate correlation pattern between these substances, pain, and metabolic status together with relative blood flow changes reported in our previous paper (Eur J Appl Physiol 108:657-669, 2010). METHODS: 43 female workers with (TM) and 19 females without (CON) trapezius myalgia were - using microdialysis - compared for differences in interstitial concentrations of interleukin-6 (IL-6), bradykinin (BKN), serotonin (5-HT), lactate dehydrogenas (LDH), substance P, and N-terminal propeptide of procollagen type I (PINP) in the trapezius muscle at rest and during repetitive/stressful work...
2014: BMC Musculoskeletal Disorders
Zhen Wang, Hui Zhang, Yan Zhang, Fei Guo, Yu Zhang, Ruihua Chen, Ru Meng, Zhiqiang Li, Qian Zhang, Chuangfu Chen
OBJECTIVE: We studied the biological function of Brucella phosphoglucomutase (pgm) gene, and detected the changes of human trophoblast cell invaded by the Brucella pgm mutant and PGM protein. METHODS: Human trophoblast cells were infected by the pgm mutant and PGM protein. The changes of cytokines were detected by enzyme-linked immunosorbent assay, and morphology of cells was identified. RESULTS: PGM protein was purified, and pgm mutant was constructed...
August 4, 2012: Wei Sheng Wu Xue Bao, Acta Microbiologica Sinica
Liping Zhao, Wenhui Yan, Heng Xiang, Xiaoyang Wang, Haixuan Qiao
The mechanisms of exercise-induced fatigue have not been investigated using proteomic techniques, an approach that could improve our understanding and generate novel information regarding the effects of exercise. In this study, the proteom alterations of rat skeletal muscle were investigated during exercise-induced fatigue. The proteins were extracted from the skeletal muscle of SD rat thigh, and then analyzed by two-dimensional electrophoresis and PDQuest software. Compared to control samples, 10 significantly altered proteins were found in exercise samples, two of them were upregulated and eight of them were downregulated...
2012: Biological Research
Ben Liu, Qing Chen, Jiang Guo, Hao Zhou, Bin Wang, Jin Li, Shou-yi Yu
OBJECTIVE: To study the anti-inflammatory effect of resveratrol in primary cortical astrocyte cultures stimulated by lipopolysaccharide (LPS) and explore the underlying mechanism of this protective effect. METHODS: The astrocytes were cultured in the presence of resveratrol at different concentrations for 12 h followed by stimulation with lipopolysaccharide (LPS) for another 24 h. Lactate dehydrogenas (LDH) leakage volumes were detected, the cytotoxicity of resveratrol was examined using cell counting kit-8 (CCK-8), the release of NO was measured by Griess reaction, and the expression levels of TNF-α and iNOS were measured using ELISA and Western blotting respectively...
December 2011: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
Ting-Ge Zhu, Xiao-Xia Wang, Wei-Feng Luo, Qi-Lin Zhang, Ting-Ting Huang, Xing-Shun Xu, Chun-Feng Liu
There is evidence to support that oxidative stress is increased in Parkinson's disease (PD) and contributes to the degeneration of dopaminergic neurons. Recent research has shown that higher blood urate concentrations have now been linked to decreased risks and progression rates of PD. However, the mechanisms about urate to protect dopaminergic neurons are less clear. Our study investigated the effect of urate on oxidative stress induced by 6-hydroxydopamine (6-OHDA) in neuronal differentiated PC12 cells. We found that urate significantly reduced 6-OHDA-induced lactate dehydrogenas (LDH), malondialdehyde (MDA), and 8-hydroxy-deoxyguanosine (8-OHdG) generation but increased the superoxide dismutase (SOD) activity and glutathione (GSH) levels in the PC12 cells...
January 11, 2012: Neuroscience Letters
Xiang-yu Zhang, Zhi-fen Yu, Da-zhao Wang, Ying Liu, Mao-ding Guo
OBJECTIVE: To investigate the effect of lemon peel extracts (LPE) on the activity of lactate dehydrogenase and sucrase of Streptococcus mutans (Sm). METHODS: After serial dilution with trypticase soy broth (TSB) medium containing 2% glucose, LPE was used as the experimental group, and TSB without LPE as the control group. Sm was added to each group, which was then cultured for 6, 18, 24 and 48 hours in the anaerobic tank. The activity of lactate dehydrogenase(LDH) was measured with the method of oxidation of reduction coenzymeIand the pH value of the culture solution was also detected...
December 2010: Zhonghua Kou Qiang Yi Xue za Zhi, Zhonghua Kouqiang Yixue Zazhi, Chinese Journal of Stomatology
No abstract text is available yet for this article.
September 1946: Journal of Biological Chemistry
T Sakai, M Kawaguchi, Y Kosuge
OBJECTIVES: siRNA-induced gene silencing in the salivary gland using microbubble-enhanced sonoporation was used to develop an in vivo gene knockdown technique. METHODS: siRNA targeting rat glyceraldehyde-3-phosphate dehydrogenas (GAPDH) was mixed with echo-enhanced microbubbles and reverse-injected into rat parotid glands using transdermal ultrasound. To compare direct and transdermal ultrasound efficiencies, an incision was made on the lateral neck to expose the parotid glands for direct application...
October 2009: Oral Diseases
Osao Adachi, Yoshitaka Ano, Hirohide Toyama, Kazunobu Matsushita
NADP-Dependent shikimate dehydrogenae (SKDH, EC was purified from Gluconobacter oxydans IFO 3244. SKDH showed a single protein band on native-PAGE accompanying enzyme activity. It required NADP exclusively and catalyzed only the shuttle reaction between shikimate and 3-dehydroshikimate. The optimum pH for shikimate oxidation and 3-dehydroshikimate reduction was found at pH 10 and 7 respectively. SKDH proved to be a useful catalyst for shikimate production from 3-dehydroshikimate.
November 2006: Bioscience, Biotechnology, and Biochemistry
C Gretzer, K Gisselfält, E Liljensten, L Rydén, P Thomsen
Early interactions between materials and mononuclear cells may influence the viability and secretory response of the cells. Such effects may in turn influence the subsequent inflammatory and repair phases around the materials. In the present study, it was examined if mononuclear cells cultured in vitro either unstimulated or stimulated with lipopolysaccharide (LPS) (10ng/ml) revealed differences regarding cell viability and apoptosis. A major interest was to study the influence of different material properties on the parameters of the inflammatory response upon cell adhesion to materials with widely different surface chemical properties but similar surface topography: degradable poly(urethane urea) (PUUR), cell culture treated polystyrene (PS) surfaces, and commercially pure (c...
August 2003: Biomaterials
R Lindahl
The subcellular distribution and properties of four aldehyde dehydrogenase isozymes (I-IV) identified in 2-acetylaminofluorene-induced rat hepatomas and three aldehyde dehydrogenase (I-III) identified in normal rat liver are compared. In normal liver, mitochondria (50%) and microsomes (27%) possess the majority of the aldehyde dehydrogenase (AlDH), with cytosol possessing little activity. Isozymes I-III can be identified in both fractions and can be differentiated on the basis of substrate and coenzyme specificity, substrate Km, inhibition by disulfiram and anti-hepatoma aldehyde dehydrogenase sera, and/or isoelectric point...
1980: Advances in Experimental Medicine and Biology
D O Nutter, N P Trujillo, J M Evans
No abstract text is available yet for this article.
September 1966: American Heart Journal
H F Fisher, D L Adija, D G Cross
No abstract text is available yet for this article.
November 1969: Biochemistry
B A Rubin, E R Kartashova, E Iu Zolotukhina
No abstract text is available yet for this article.
June 1, 1975: Doklady Akademii Nauk SSSR
G G Shurda, L E Panin
A modified method is described for estimation of hexokinase isoenzymes by polyacrylamide gel electrophoresis. The best conditions for detection of hexokinase activity were developed using immobilization of glucose-6-phosphate dehydrogenas: in gel, utilization of riboflavin as a catalyst of polymerization and stabilization by glucose. The method is simple, highly reproducible, comparatively rapid and economically reasonable.
March 1978: Voprosy Medit︠s︡inskoĭ Khimii
G Falkay, M Sas
No abstract text is available yet for this article.
January 1978: Journal of Endocrinology
R Prignitz, N Hoffmeister, G Hoffmeister
The behavior of enzyme activities, substrates and metabolites of glycosis as well as of the pentose phosphate shunt following local irradiation (250 to 6000 R surface dose) is biochemically investigated in the guinea-pig's myocardium. During irradiation, an activation of phosphorylase-a is going on while the total phosphorylase content remains unchanged. Enzyme activities of hexokinase and phosphofructokinase are increased in dependence on dosage as well as time. The glycogen content is being reduced; tissular concentration of the metabolites glucose-1-phosphate, glucose-6-phosphate, glyceraldehyde-3-phosphate, glycerol-3-phosphate, and pyruvate increases following irradiation; the content of fructose-1,6-diphosphate, dihydroxyacetonephosphate, and lactate is decreased...
June 1975: Strahlentherapie
A Gafni, L Brand
The monophoton counting technique was used to obtain the fluorescence decay kinetics of NADH (dihydronicotinamide adenine dinucleotide) bound to LADH (HORSE LIVER ALCOHOL DEHYDROGENAS). It was found that the fluorescence decay of the enzyme complex did not follow a single exponential decay law but that the data could be well described as a sum of two exponentials. The decay parameters of the enzyme complex do not depend on the degree of binding-site saturation. These results are interpreted in terms of a reversible excited-state reaction forming a nonfluorescent product...
July 27, 1976: Biochemistry
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