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Cofactor regeneration

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https://www.readbyqxmd.com/read/28526345/facile-fabrication-of-3d-porous-hybrid-sphere-by-co-immobilization-of-multi-enzyme-directly-from-cell-lysates-as-an-efficient-and-recyclable-biocatalyst-for-asymmetric-reduction-with-coenzyme-regeneration-in-situ
#1
MinganYu, Duqiang Liu, Lili Sun, Jing Li, Qian Chen, Lijun Pan, Jingchuan Shang, Shurong Zhang, Wei Li
Ni(2+)-agarose bead-wrapped multi-enzyme/inorganic hybrid sphere composed of the immobilized enzymes as organic component and NaH2PO4 and NaCl as inorganic component was developed by co-immobilizing extracellular His-tagged 3-quinuclidinone reductases and glucose dehydrogenase without pre-purification. The resulting biocatalysts has 3D porous architectures as confirmed by SEM and FESEM, and it enabled the continuous biotransformation of 3-quinuclidone to (R)-3-quinuclidinol with cofactor regeneration in situ...
May 16, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28512473/vitamin-c-in-stem-cell-biology-impact-on-extracellular-matrix-homeostasis-and-epigenetics
#2
REVIEW
Cristina D'Aniello, Federica Cermola, Eduardo Jorge Patriarca, Gabriella Minchiotti
Transcription factors and signaling molecules are well-known regulators of stem cell identity and behavior; however, increasing evidence indicates that environmental cues contribute to this complex network of stimuli, acting as crucial determinants of stem cell fate. l-Ascorbic acid (vitamin C (VitC)) has gained growing interest for its multiple functions and mechanisms of action, contributing to the homeostasis of normal tissues and organs as well as to tissue regeneration. Here, we review the main functions of VitC and its effects on stem cells, focusing on its activity as cofactor of Fe(+2)/αKG dioxygenases, which regulate the epigenetic signatures, the redox status, and the extracellular matrix (ECM) composition, depending on the enzymes' subcellular localization...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28498544/metabolic-engineering-of-cofactor-flavin-adenine-dinucleotide-fad-synthesis-and-regeneration-in-escherichia-coli-for-production-of-%C3%AE-keto-acids
#3
Ying Hou, Gazi Sakir Hossain, Jianghua Li, Hyun-Dong Shin, Guocheng Du, Jian Chen, Long Liu
Cofactor flavin adenine dinucleotide (FAD) plays a vital role in many FAD-dependent enzymatic reactions; therefore, how to efficiently accelerate FAD synthesis and regeneration is an important topic in biocatalysis and metabolic engineering. In this study, a system involving the synthesis pathway and regeneration of FAD was engineered in Escherichia coli to improve α-keto acid production-from the corresponding L-amino acids-catalyzed by FAD-dependent L-amino acid deaminase (L-AAD). First, key genes, ribH, ribC, and ribF, were overexpressed and fine-tuned for FAD synthesis...
May 12, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28461497/folate-rescues-vitamin-b12-depletion-induced-inhibition-of-nuclear-thymidylate-biosynthesis-and-genome-instability
#4
Ashley M Palmer, Elena Kamynina, Martha S Field, Patrick J Stover
Clinical vitamin B12 deficiency can result in megaloblastic anemia, which results from the inhibition of DNA synthesis by trapping folate cofactors in the form of 5-methyltetrahydrofolate (5-methylTHF) and subsequent inhibition of de novo thymidylate (dTMP) biosynthesis. In the cytosol, vitamin B12 functions in the remethylation of homocysteine to methionine, which regenerates THF from 5-methylTHF. In the nucleus, THF is required for de novo dTMP biosynthesis, but it is not understood how 5-methylTHF accumulation in the cytosol impairs nuclear dTMP biosynthesis...
May 1, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28427921/enhancement-of-ethyl-s-4-chloro-3-hydroxybutanoate-production-at-high-substrate-concentration-by-in-situ-resin-adsorption
#5
Li-Feng Chen, Hai-Yang Fan, Yi-Ping Zhang, Wei Wei, Jin-Ping Lin, Dong-Zhi Wei, Hua-Lei Wang
Asymmetric reduction of ethyl 4-chloro-3-oxobutyrate (COBE) by carbonyl reductases presents an efficient way to produce Ethyl (S)-4-chloro-3-hydroxybutanoate ((S)-CHBE), an important chiral intermediate for the synthesis of hydroxymethylglutaryl-CoA reductase inhibitors such as Lipitor(®). In this study, an NADPH-dependent carbonyl reductase (SrCR) from Synechocystis sp. was characterized to demonstrate a broad substrate spectrum, and the highest activity (53.1U/mg protein) with COBE. To regenerate the cofactor NADPH, Bacillus subtilis glucose dehydrogenase was successfully coexpressed with SrCR...
April 17, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28423958/strategies-and-perspectives-of-assembling-multi-enzyme-systems
#6
Shi-Zhen Wang, Yong-Hui Zhang, Hong Ren, Ya-Li Wang, Wei Jiang, Bai-Shan Fang
Multi-enzyme complexes have the potential to achieve high catalytic efficiency for sequence reactions due to their advantages in eliminating product inhibition, facilitating intermediate transfer and in situ regenerating cofactors. Constructing functional multi-enzyme systems to mimic natural multi-enzyme complexes is of great interest for multi-enzymatic biosynthesis and cell-free synthetic biotransformation, but with many challenges. Currently, various assembly strategies have been developed based on the interaction of biomacromolecules such as DNA, peptide and scaffolding protein...
April 20, 2017: Critical Reviews in Biotechnology
https://www.readbyqxmd.com/read/28401536/autodisplay-of-glucose-6-phosphate-dehydrogenase-for-redox-cofactor-regeneration-at-the-cell-surface
#7
Jan Schüürmann, Paul Quehl, Fabian Lindhorst, Kristina Lang, Joachim Jose
Inherent cofactor regeneration is a pivotal feature of whole cell biocatalysis. For specific biotechnological applications, surface display of enzymes is emerging as a tool to circumvent mass transfer limitations or enzyme stability problems. Even complex reactions can be accomplished applying displayed enzymes. Yet, industrial utilization of the technique is still impeded by lacking cofactor regeneration at the cell surface. Here, we report on the surface display of a glucose-6-phoshate dehydrogenase (G6PDH) via Autodisplay to address this limitation and regenerate NADPH directly at the cell surface...
April 12, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28389394/kinetically-accessible-yield-kay-for-redirection-of-metabolism-to-produce-exo-metabolites
#8
Jimmy G Lafontaine Rivera, Matthew K Theisen, Po-Wei Chen, James C Liao
The product formation yield (product formed per unit substrate consumed) is often the most important performance indicator in metabolic engineering. Until now, the actual yield cannot be predicted, but it can be bounded by its maximum theoretical value. The maximum theoretical yield is calculated by considering the stoichiometry of the pathways and cofactor regeneration involved. Here we found that in many cases, dynamic stability becomes an issue when excessive pathway flux is drawn to a product. This constraint reduces the yield and renders the maximal theoretical yield too loose to be predictive...
April 5, 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/28387366/elucidating-dynamic-metabolic-physiology-through-network-integration-of-quantitative-time-course-metabolomics
#9
Aarash Bordbar, James T Yurkovich, Giuseppe Paglia, Ottar Rolfsson, Ólafur E Sigurjónsson, Bernhard O Palsson
The increasing availability of metabolomics data necessitates novel methods for deeper data analysis and interpretation. We present a flux balance analysis method that allows for the computation of dynamic intracellular metabolic changes at the cellular scale through integration of time-course absolute quantitative metabolomics. This approach, termed "unsteady-state flux balance analysis" (uFBA), is applied to four cellular systems: three dynamic and one steady-state as a negative control. uFBA and FBA predictions are contrasted, and uFBA is found to be more accurate in predicting dynamic metabolic flux states for red blood cells, platelets, and Saccharomyces cerevisiae...
April 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28357799/what-about-the-detoxification-mechanisms-underlying-ozone-sensitivity-in-liriodendron-tulipifera
#10
Elisa Pellegrini, Alessandra Campanella, Lorenzo Cotrozzi, Mariagrazia Tonelli, Cristina Nali, Giacomo Lorenzini
Liriodendron tulipifera (known as the tulip tree) is a woody species that has been previously classified as sensitive to ozone (O3) in terms of visible leaf injuries and photosynthetic primary reactions. The objective of this work is to give a thorough description of the detoxification mechanisms that are at the basis of O3 sensitivity. Biochemical and molecular markers were used to characterize the response of 1-year-old saplings exposed to O3 (120 ppb, 5 h day(-1), for 45 consecutive days) under controlled conditions...
March 29, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28346008/wnt-%C3%AE-catenin-signaling-is-required-for-distraction-osteogenesis-in-rats
#11
Xuemei Wang, En Luo, Ruiye Bi, Bin Ye, Jing Hu, Shujuan Zou
OVERVIEW: The Wnt signaling pathway plays crucial roles in embryonic skeletal development and postnatal bone regeneration. However, mechanisms of Wnt signaling functioning in distraction osteogenesis (DO) haven't been well characterized. MATERIALS AND METHODS: We established a DO model using Sprague-Dawley rat tibia. And a Wnt signaling blocking agent, recombinant rat Dickkopf-related protein 1 (rrDkk1), was locally applied in the distracted gap to study the role of Wnt signaling during DO process...
March 1, 2017: Connective Tissue Research
https://www.readbyqxmd.com/read/28345146/evidence-for-the-generation-of-myristylated-fmn-by-bacterial-luciferase
#12
Chaitanya R Tabib, Eveline Brodl, Peter Macheroux
The genes responsible for the light production in bioluminescent bacteria are present as an operon, luxCDABEG. Many strains of Photobacteria carry an additional gene, termed luxF. X-ray crystallographic analysis of LuxF revealed the presence of four molecules of a flavin derivative, i.e. 6-(3'-(R)-myristyl) flavin adenine mononucleotide (myrFMN) non-covalently bound to the homodimer. In the present study, we exploited the binding of myrFMN to recombinant apo-LuxF to explore the occurrence of myrFMN in various bioluminescent bacteria...
March 27, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28329449/behavioural-and-neurochemical-effects-of-alpha-lipoic-acid-associated-with-omega-3-in-tardive-dyskinesia-induced-by-chronic-haloperidol-in-rats
#13
Dayane Pessoa Araújo, Thaisa Camboim, Ana Silva, Caio Fonseca, Rebeca Sousa, Mabson Barbosa, Lucidio Oliveira, José Cavalcanti, Eudes Lucena, Fausto Guzen
Tardive dyskinesia (TD) is characterized by involuntary movements of the lower portion of the face being related to typical antipsychotic therapy. TD is associated with the oxidative imbalance in the basal ganglia. Lipoic acid (LA) and omega 3 (ω-3) are antioxidants acting as enzyme cofactors, regenerating antioxidant enzymes. This study aimed to investigate behavioral and neurochemical effects of supplementation with of LA (100mg/Kg) and ω-3 (1g/Kg) in the treatment of TD-induced by chronic use haloperidol (HAL) (1mg/Kg) in rats...
March 22, 2017: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/28230096/a-novel-approach-to-improve-poly-%C3%AE-glutamic-acid-production-by-nadph-regeneration-in-bacillus-licheniformis-wx-02
#14
Dongbo Cai, Penghui He, Xingcheng Lu, Chengjun Zhu, Jiang Zhu, Yangyang Zhan, Qin Wang, Zhiyou Wen, Shouwen Chen
Poly-γ-glutamic acid (γ-PGA) is an important biochemical product with a variety of applications. This work reports a novel approach to improve γ-PGA through over expression of key enzymes in cofactor NADPH generating process for NADPH pool. Six genes encoding the key enzymes in NADPH generation were over-expressed in the γ-PGA producing strain B. licheniformis WX-02. Among various recombinants, the strain over-expressing zwf gene (coding for glucose-6-phosphate dehydrogenase), WX-zwf, produced the highest γ-PGA concentration (9...
February 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28193327/cloning-expression-and-biochemical-characterization-of-a-novel-nadp-dependent-7%C3%AE-hydroxysteroid-dehydrogenase-from-clostridium-difficile-and-its-application-for-the-oxidation-of-bile-acids
#15
Daniel Bakonyi, Werner Hummel
A gene encoding a novel 7α-specific NADP(+)-dependent hydroxysteroid dehydrogenase from Clostridium difficile was cloned and heterologously expressed in Escherichia coli. The enzyme was purified using an N-terminal hexa-his-tag and biochemically characterized. The optimum temperature is at 60°C, but the enzyme is inactivated at this temperature with a half-life time of 5min. Contrary to other known 7α-HSDHs, for example from Clostridium sardiniense or E. coli, the enzyme from C. difficile does not display a substrate inhibition...
April 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/28183178/mimicking-horseradish-peroxidase-and-nadh-peroxidase-by-heterogeneous-cu-2-modified-graphene-oxide-nanoparticles
#16
Shan Wang, Rémi Cazelles, Wei-Ching Liao, Margarita Vázquez-González, Amani Zoabi, Raed Abu-Reziq, Itamar Willner
Cu(2+)-ion-modified graphene oxide nanoparticles, Cu(2+)-GO NPs, act as a heterogeneous catalyst mimicking functions of horseradish peroxidase, HRP, and of NADH peroxidase. The Cu(2+)-GO NPs catalyze the oxidation of dopamine to aminochrome by H2O2 and catalyze the generation of chemiluminescence in the presence of luminol and H2O2. The Cu(2+)-GO NPs provide an active material for the chemiluminescence detection of H2O2 and allow the probing of the activity of H2O2-generating oxidases and the detection of their substrates...
March 8, 2017: Nano Letters
https://www.readbyqxmd.com/read/28176878/cofactor-recycling-for-co-production-of-1-3-propanediol-and-glutamate-by-metabolically-engineered-corynebacterium-glutamicum
#17
Jinhai Huang, Yao Wu, Wenjun Wu, Ye Zhang, Dehua Liu, Zhen Chen
Production of 1,3-propanediol (1,3-PDO) from glycerol is a promising route toward glycerol biorefinery. However, the yield of 1,3-PDO is limited due to the requirement of NADH regeneration via glycerol oxidation process, which generates large amounts of undesired byproducts. Glutamate fermentation by Corynebacterium glutamicum is an important oxidation process generating excess NADH. In this study, we proposed a novel strategy to couple the process of 1,3-PDO synthesis with glutamate production for cofactor regeneration...
February 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28170142/catalytic-alkylation-using-a-cyclic-s-adenosylmethionine-regeneration-system
#18
Silja Mordhorst, Jutta Siegrist, Michael Müller, Michael Richter, Jennifer N Andexer
S-Adenosylmethionine-dependent methyltransferases are versatile tools for the specific alkylation of many compounds, such as pharmaceuticals, but their biocatalytic application is severely limited owing to the lack of a cofactor regeneration system. We report a biomimetic, polyphosphate-based, cyclic cascade for methyltransferases. In addition to the substrate to be methylated, only methionine and polyphosphate have to be added in stoichiometric amounts. The system acts catalytically with respect to the cofactor precursor adenosine in methylation and ethylation reactions of selected substrates, as shown by HPLC analysis...
February 7, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28163781/enhancement-of-furan-aldehydes-conversion-in-zymomonas-mobilis-by-elevating-dehydrogenase-activity-and-cofactor-regeneration
#19
Xia Wang, Qiuqiang Gao, Jie Bao
BACKGROUND: Furfural and 5-hydroxymethylfurfural (HMF) are the two major furan aldehyde inhibitors generated from lignocellulose dilute acid pretreatment which significantly inhibit subsequent microbial cell growth and ethanol fermentation. Zymomonas mobilis is an important strain for cellulosic ethanol fermentation but can be severely inhibited by furfural and (or) HMF. Previous study showed that Z. mobilis contains its native oxidoreductases to catalyze the conversion of furfural and HMF, but the corresponding genes have not been identified...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28120367/photoelectrochemical-reduction-of-carbon-dioxide-to-methanol-through-a-highly-efficient-enzyme-cascade
#20
Su Keun Kuk, Raushan K Singh, Dong Heon Nam, Ranjitha Singh, Jung-Kul Lee, Chan Beum Park
Natural photosynthesis is an effective route for the clean and sustainable conversion of CO2 into high-energy chemicals. Inspired by the natural process, a tandem photoelectrochemical (PEC) cell with an integrated enzyme-cascade (TPIEC) system was designed, which transfers photogenerated electrons to a multienzyme cascade for the biocatalyzed reduction of CO2 to methanol. A hematite photoanode and a bismuth ferrite photocathode were applied to fabricate the iron oxide based tandem PEC cell for visible-light-assisted regeneration of the nicotinamide cofactor (NADH)...
January 25, 2017: Angewandte Chemie
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