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Grace Thiele, Conne Friedman, Kathleen Tsai, Joris Beld, Casey H Londergan, Louise K Charkoudian
Acyl carrier proteins (ACPs) are central hubs in polyketide and fatty acid biosynthetic pathways, but the fast motions of the ACP's phosphosphopantetheine (Ppant) arm make its conformational dynamics difficult to capture using traditional spectroscopic approaches. Here we report that converting the terminal thiol of E. coli ACP's Ppant arm into a thiocyanate activates this site to form a selective crosslink with the active site cys-teine of its partner ketoacyl synthase (KS; FabF). The reaction releases a cyanide anion, which can be detected by infrared spectroscopy...
April 27, 2017: Biochemistry
M Rita Correro, Yves Dudal, Philippe F-X Corvini, Patrick Shahgaldian
A series of synthetic nanomaterials capable of molecular recognition and/or biocatalysis have been produced by exploiting the self-sorting, self-assembly and polycondensation of organosilane building blocks around protein templates. The established methodology allows for the production of thin organosilica layers of controlled thickness, down to nanometer precision. Fully synthetic virus recognition materials have been shown to specifically bind their target virus down to picomolar concentrations. The shielding of natural enzymes allowed producing nanobiocatalysts functioning under harsh operational conditions...
April 26, 2017: Chimia
Jinglin Fu
Recently, peptide microarrays have been used to distinguish proteins, antibodies, viruses, and bacteria based on their binding to random sequence peptides. We reported on the use of peptide arrays to identify enzyme modulators that involve screening an array of 10,000 defined and addressable peptides on a microarray. Primary peptides were first selected to inhibit the enzyme at low μM concentrations. Then, new peptides were found to only bind strongly with the enzyme-inhibitor complex, but not the native enzyme...
April 26, 2017: Microarrays
Rik K Wierenga, Dagmar Ringe
No abstract text is available yet for this article.
April 24, 2017: Protein Engineering, Design & Selection: PEDS
Xiaoling Wu, Gang Ou, Cheng Yang, Jun Ge, Hui Wu
In this study, we show that enzymatic reactions can be easily enhanced by visible light irradiation. It is a general method as demonstrated by several different types of enzymes, including amylase, cellulase and lipase, which are typically utilized in many industrial biocatalysis processes. Markedly different from conventional titanium dioxide (TiO2) nanoparticles which absorb UV light and generate little thermal energy, defect-rich Magnéli phase titanium oxide (Ti8O15) nanoparticles absorb solar light and provide a highly effective solar-to-thermal conversion, which greatly enhances enzymatic reactions...
April 21, 2017: Chemical Communications: Chem Comm
Miao Hou, Jun Ge
Enzymatic processes have great potential for applications in industrial biocatalysis, biosensing, and biomedical engineering. However, poor stability and difficulty in the reuse of enzymes still create challenges for their use in catalysis and other applications. In recent years, enzyme-incorporated metal-organic frameworks (enzyme@MOF) have emerged as a new type of armored enzymes and are thought to be an appealing solution to the above challenges. To date, the preparation of enzyme-MOF composites can be divided into three categories, including physical adsorption, covalent conjugation, and coprecipitation...
2017: Methods in Enzymology
Yi Liu, Aoneng Cao
Encapsulation of proteins in nanoparticles (NPs) can greatly improve the properties of proteins such as their stability against denaturation and degradation by proteases, and branches out the applications of natural proteins from their intrinsic localizations and functions in living organisms for biomedical and industrial applications. We recently developed several methods to armor proteins in NPs with sizes from nanometers up to >100nm, batch by batch or one by one, covalently or noncovalently, for a wide range of applications from biocatalysis to bioimaging and drug delivery...
2017: Methods in Enzymology
Hemant Kumar, Quoc-Thai Nguyen, Claudia Binda, Andrea Mattevi, Marco W Fraaije
F420H2-dependent enzymes reduce a wide range of substrates that are otherwise recalcitrant to enzyme-catalyzed reduction, and their potential for applications in biocatalysis has attracted increasingly attention. Thermobifida fusca is a moderately thermophilic bacterium and holds high biocatalytic potential as a source for several highly thermostable enzymes. We report here on the isolation and characterization of a thermostable F420:NADPH oxidoreductase (Tfu-FNO) from T. fusca, being the first F420-dependent enzyme described from this bacterium...
April 14, 2017: Journal of Biological Chemistry
Erick Moran Carreira, Nicholas Turner, Moritz Hönig, Philipp Sondermann
Recent developments of biocatalytic and chemocata-lytic methods are discussed. The review provides a guide to the use of biocatalytic methods in the area of chemical synthesis with focused attention on retrosynthetic considerations and analysis. The methods presented are organized according to bond disconnections and attendant synthetic methods. The review is expected to lead to better understanding of the charac-teristics and distinctions of the two complementary approaches. It depicts for researchers in bio- and chemocatalysis a road map of challenges and opportunities for the evolution (and at times revolution) in chemical synthesis...
April 13, 2017: Angewandte Chemie
Ren Wei, Wolfgang Zimmermann
Biocatalysis can enable a closed-loop recycling of post-consumer PET waste.
April 12, 2017: Microbial Biotechnology
Jan Schüürmann, Paul Quehl, Fabian Lindhorst, Kristina Lang, Joachim Jose
Inherent cofactor regeneration is a pivotal feature of whole cell biocatalysis. For specific biotechnological applications, surface display of enzymes is emerging as a tool to circumvent mass transfer limitations or enzyme stability problems. Even complex reactions can be accomplished applying displayed enzymes. Yet, industrial utilization of the technique is still impeded by lacking cofactor regeneration at the cell surface. Here, we report on the surface display of a glucose-6-phoshate dehydrogenase (G6PDH) via Autodisplay to address this limitation and regenerate NADPH directly at the cell surface...
April 12, 2017: Biotechnology and Bioengineering
Luan Luong Chu, Ramesh Prasad Pandey, Haet Nim Lim, Hye Jin Jung, Nguyen Huy Thuan, Tae-Su Kim, Jae Kyung Sohng
BACKGROUND: Umbelliferone, also known as 7-hydroxycoumarin, is a phenolic metabolite found in many familiar plants. Its derivatives have been shown to have various pharmacological and chemo-preventive effects on human health. A uridine diphosphate glycosyltransferase YjiC from Bacillus licheniformis DSM 13, a cytochrome P450BM3 (CYP450 BM3) variant namely mutant 13 (M13) from Bacillus megaterium, and an O-methyltransferase from Streptomyces avermitilis (SaOMT2) were used for modifications of umbelliferone...
2017: Journal of Biological Engineering
Danilo Degregorio, Serena D'Avino, Silvia Castrignanò, Giovanna Di Nardo, Sheila J Sadeghi, Gianluca Catucci, Gianfranco Gilardi
Human liver cytochrome P450 3A4 is the main enzyme involved in drug metabolism. This makes it an attractive target for biocatalytic applications, such as the synthesis of pharmaceuticals and drug metabolites. However, its poor solubility, stability and low coupling have limited its application in the biotechnological context. We previously demonstrated that the solubility of P450 3A4 can be increased by creating fusion proteins between the reductase from Bacillus megaterium BM3 (BMR) and the N-terminally modified P450 3A4 (3A4-BMR)...
2017: Frontiers in Pharmacology
Calvin A Henard, Holly K Smith, Michael T Guarnieri
Microbial conversion of methane to high-value bio-based fuels, chemicals, and materials offers a path to mitigate GHG emissions and valorize this abundant-yet -underutilized carbon source. In addition to fermentation optimization strategies, rational methanotrophic bacterial strain engineering offers a means to reach industrially relevant titers, carbon yields, and productivities of target products. The phosphoketolase pathway functions in heterofermentative bacteria where carbon flux through two sugar catabolic pathways to mixed acids (lactic acid and acetic acid) increases cellular ATP production...
April 2, 2017: Metabolic Engineering
Huan Wang, Hongcheng Gu, Zhuoyue Chen, Luoran Shang, Ze Zhao, Zhongze Gu, Yuanjin Zhao
Enzymatic carriers have a demonstrated value for chemical reactions and industrial applications. Here, we present a novel kind of inverse opal hydrogel particles as the enzymatic carriers. The particles were negatively replicated from spherical colloidal crystal templates by using magnetic nanoparticles tagged acrylamide hydrogel. Thus, they were endowed with the features of monodispersity, small volume, complete penetrating structure, and controllable motion, which are all beneficial for improving the efficiency of biocatalysis...
April 6, 2017: ACS Applied Materials & Interfaces
Abraham J Waldman, Tai L Ng, Peng Wang, Emily P Balskus
Natural products that contain functional groups with heteroatom-heteroatom linkages (X-X, where X = N, O, S, and P) are a small yet intriguing group of metabolites. The reactivity and diversity of these structural motifs has captured the interest of synthetic and biological chemists alike. Functional groups containing X-X bonds are found in all major classes of natural products and often impart significant biological activity. This review presents our current understanding of the biosynthetic logic and enzymatic chemistry involved in the construction of X-X bond containing functional groups within natural products...
April 4, 2017: Chemical Reviews
M B Quin, K K Wallin, G Zhang, C Schmidt-Dannert
Industrial biocatalysis is an economically attractive option for the production of valuable chemicals. Our repertoire of cheap building blocks and commodity target molecules is vastly enhanced by multi-enzyme biocatalytic cascades. In order to achieve suitable titers in complex novel biocatalytic schemes, spatial organization may become necessary to overcome barriers caused by slow or inhibited enzymes as well as instability of biocatalysts. A number of spatial organization strategies are currently available, which could be integrated in the design of complex cascades...
April 4, 2017: Organic & Biomolecular Chemistry
Mark B Frampton, Paul M Zelisko
The application of biocatalytic or chemoenzymatic techniques in silicon chemistry serves two roles. It provides a greater understanding of the processing of silicon species by natural systems such as plants, diatoms, and sponges as well opening avenues to green methodologies in the field. In the latter case biocatalytic approaches have been applied to the synthesis of small molecule systems as well as polymeric materials. Often these biocatalytic approaches allow access to molecular structures under mild conditions and, in some cases, molecular structures that are not accessible through traditional chemical methodologies...
March 29, 2017: Chemistry, An Asian Journal
Prem C Pandey, Govind Pandey, Roger J Narayan
Mesoporous silica nanoparticles (MSNPs) have been used as an efficient and safe carrier for drug delivery and biocatalysis. The surface modification of MSNPs using suitable reagents may provide a robust framework in which two or more components can be incorporated to give multifunctional capabilities (e.g., synthesis of noble metal nanoparticles within mesoporous architecture along with loading of a bioactive molecule). In this study, the authors reported on a new synthetic route for the synthesis of gold nanoparticles (AuNPs) within (1) unmodified MSNPs and (2) 3-trihydroxysilylpropyl methylphosphonate-modified MSNPs...
March 27, 2017: Biointerphases
L M Kulishova, D O Zharkov
Solid/gas biocatalysis is a nontraditional reaction system that employs the ability of some enzymes, being in the solid state, to catalyze reactions of substrates in the gas phase. Manipulation of the reaction parameters (temperature and pressure) in the solid/gas system allows precise control over the thermodynamic activity of water and substrate and creation of a controlled microenvironment for the enzyme, making it an appropriate model for enzymology studies. Owing to such advantages as high stability of dry enzymes and cofactors and easy fractionation of gas mixtures, solid/gas biotechnology has already found several industrial applications...
February 2017: Biochemistry. Biokhimii︠a︡
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