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Ryan Tullius, Geoffrey W Platt, Larousse Khosravi Khorashad, Nikolaj Gadegaard, Adrian J Lapthorn, Vincent M Rotello, Graeme Cooke, Laurence D Barron, Alexander O Govorov, Affar Shahid Karimullah, Malcolm Kadodwala
The structure adopted by biomaterials, such as proteins, at interfaces is a crucial parameter in a range of important biological problems. It is a critical property in defining the functionality of cell / bacterial membranes, biofilms (i.e. in antibiotic resistant infections) and the exploitation of immobilised enzymes in biocatalysis. The intrinsically small quantities of materials at interfaces precludes the application of conventional spectroscopic phenomena routinely used for (bio)structural analysis due to a lack of sensitivity...
December 8, 2017: ACS Nano
Marc Riedel, Fred Lisdat
Inspired by natural photosynthesis, coupling of artificial light-sensitive entities with biocatalysts in a biohybrid format can result in advanced photobioelectronic systems. Herein, we report on the integration of sulfonated polyanilines (PMSA1) and PQQ glucose dehydrogenase (PQQ-GDH) into inverse opal TiO2 (IO-TiO2) electrodes. While PMSA1 introduces sensitivity for visible light into the biohybrid architecture and ensures the efficient wiring between the IO-TiO2 electrode and the biocatalytic entity, PQQ-GDH provides the catalytic activity for the glucose oxidation and therefore feeds the light-driven reaction with electrons for an enhanced light-to-current conversion...
December 8, 2017: ACS Applied Materials & Interfaces
C M Romero, P V Martorell, A Gómez López, C G Nieto Peñalver, S Chaves, M Mechetti
Biocatalysis for industrial application is based on the use of enzymes to perform complex transformations. However, these systems have some disadvantage related to the costs of the biocatalyst. In this work, an alternative strategy for producing green immobilized biocatalysts based on biofilm was developed.A study of the rheological behavior of the biofilm from Bacillus sp. Mcn4, as well as the determination of its composition, was carried out. The dynamic rheological measurements, viscosity (G") and elasticity (G') module, showed that the biofilm presents appreciable elastic components, which is a recognized property for enzymes immobilization...
November 23, 2017: Colloids and Surfaces. B, Biointerfaces
Jingjing Liu, Jianghua Li, Hyun-Dong Shin, Guocheng Du, Jian Chen, Long Liu
As intermediates in the TCA cycle, L-malate and its derivatives have been widely applied in the food, pharmaceutical, agriculture, and bio-based material industries. In recent years, biological routes have been regarded as very promising approaches as cost-effective ways to L-malate production from low-priced raw materials. In this mini-review, we provide a comprehensive overview of current developments of L-malate production using both biocatalysis and microbial fermentation. Biocatalysis is enzymatic transformation of fumarate to L-malate, here, the source of enzymes, catalytic conditions, and enzymatic molecular modification may be concluded...
December 6, 2017: World Journal of Microbiology & Biotechnology
Wenjun Yu, Rongshui Wang, Huili Li, Jiyu Liang, Yuanyuan Wang, Haiyan Huang, Huijun Xie, Shuning Wang
Background: Tobacco is widely planted as an important nonfood economic crop throughout the world, and large amounts of tobacco wastes are generated during the tobacco manufacturing process. Tobacco and its wastes contain high nicotine content. This issue has become a major concern for health and environments due to its toxicity and complex physiological effects. The microbial transformation of nicotine into valuable functionalized pyridine compounds is a promising way to utilize tobacco and its wastes as a potential biomass resource...
2017: Biotechnology for Biofuels
Renata Novakova, Luz Elena Núñez, Dagmar Homerova, Renata Knirschova, Lubomira Feckova, Bronislava Rezuchova, Beatrica Sevcikova, Nuria Menéndez, Francisco Morís, Jesús Cortés, Jan Kormanec
Mithramycin A is an antitumor compound used for treatment of several types of cancer including chronic and acute myeloid leukemia, testicular carcinoma, hypercalcemia and Paget's disease. Selective modifications of this molecule by combinatorial biosynthesis and biocatalysis opened the possibility to produce mithramycin analogues with improved properties that are currently under preclinical development. The mithramycin A biosynthetic gene cluster from Streptomyces argillaceus ATCC12956 was cloned by transformation assisted recombination in Saccharomyces cerevisiae and heterologous expression in Streptomyces lividans TK24 was evaluated...
December 1, 2017: Applied Microbiology and Biotechnology
Sahng Ha Lee, Da Som Choi, Su Keun Kuk, Chan Beum Park
Biocatalytic transformation has received increasing attention in green synthesis of chemicals because of the diversity of enzymes, their superior catalytic activities and specificities, and mild reaction conditions. The idea of solar energy utilization in chemical synthesis through the combination of photocatalysis and biocatalysis provides an opportunity to make the "green" process greener. Oxidoreductases catalyze redox transformation of substrates by exchanging electrons at the enzyme active site, often with the aid of electron mediator(s) as a counterpart...
November 30, 2017: Angewandte Chemie
Pál Csuka, Vivienn Juhász, Szabolcs Kohári, Alina Filip, Andrea Varga, Péter Sátorhelyi, László Csaba Bencze, Hazel A Barton, Csaba Paizs, Laszlo Poppe
A number of class I lyase-like enzymes including aromatic ammonia-lyases and aromatic 2,3-aminomutases containing the electrophilic 3,5-dihydro-5-methylidene-4H-imidazol-4-one (MIO) catalytic moiety. This study reveals that Pseudomonas fluorescens R124 strain isolated from a nutrient limited cave encodes a histidine ammonia-lyase (HAL), a tyrosine/phenylalanine/histidine ammonia-lyase (XAL) and a phenylalanine 2,3-aminomutase (PAM), and demonstrates that an organism under nitrogen-limited conditions can develop novel nitrogen fixation and transformation pathways to enrich the possibility of the nitrogen metabolism by gaining a PAM via horizontal gene transfer...
November 28, 2017: Chembiochem: a European Journal of Chemical Biology
Long-Liu Lin, Meng-Chun Chi, Eng-Gin Lan, Min-Guan Lin, Tzong-Yuan Juang, Tzu-Fan Wang
For the practical application of Bacillus licheniformis γ-glutamyltranspeptidase (BlGGT), we illustrated a simple and efficient approach to fabricate a biocatalytic system by immobilizing the enzyme onto graphene oxide (GO) nanosheets via both non-covalent (GO-BlGGT) and covalent (GO/GA-BlGGT) bonds. The enzyme-loading capacity for the prepared GO/GA nanomaterial was 3.47mg/mg support, corresponding to 68.7% recovery of the initial activity. Native and enzyme-bound layered GOs were characterized by X-ray diffraction, followed by Raman and Fouier transform infrared spectroscopy, elemental analysis and thermogram analysis...
November 25, 2017: International Journal of Biological Macromolecules
Lidan Ye, Chengcheng Yang, Hongwei Yu
With increasing concerns in sustainable development, biocatalysis has been recognized as a competitive alternative to traditional chemical routes in the past decades. As nature's biocatalysts, enzymes are able to catalyze a broad range of chemical transformations, not only with mild reaction conditions but also with high activity and selectivity. However, the insufficient activity or enantioselectivity of natural enzymes toward non-natural substrates limits their industrial application, while directed evolution provides a potent solution to this problem, thanks to its independence on detailed knowledge about the relationship between sequence, structure, and mechanism/function of the enzymes...
November 27, 2017: Applied Microbiology and Biotechnology
Jun Ni, Yan-Yan Gao, Fei Tao, Hong-Yu Liu, Ping Xu
Biosynthesis of aromatic aldehydes/alcohols from renewable resources is currently receiving considerable attention because of increased demand, finite fossil resources and growing environmental concerns. Here, temperature-routed whole-cell catalysis was developed using two novel enzymes from a thermophilic actinomycete. Ferulic acid, a model lignin derivative, was efficiently converted to vanillyl alcohol by maintaining the reaction temperature at 30C. However, when the temperature was increased to 50C, ferulic acid was mainly converted to vanillin with a productivity of 1...
November 26, 2017: Angewandte Chemie
Lara Trobo-Maseda, Alejandro H Orrego, Sonia Moreno-Pérez, Gloria Fernández-Lorente, José M Guisan, Javier Rocha-Martin
Sucrose synthases (SuSys) have been attracting great interest in recent years in industrial biocatalysis. They can be used for the cost-effective production of uridine 5'-diphosphate glucose (UDP-glucose) or its in situ recycling if coupled to glycosyltransferases on the production of glycosides in the food, pharmaceutical, nutraceutical, and cosmetic industry. In this study, the homotetrameric SuSy from Acidithiobacillus caldus (SuSyAc) was immobilized-stabilized on agarose beads activated with either (i) glyoxyl groups, (ii) cyanogen bromide groups, or (iii) heterogeneously activated with both glyoxyl and positively charged amino groups...
November 25, 2017: Applied Microbiology and Biotechnology
John M Woodley
Biocatalysis uses enzymes for chemical synthesis and production, offering selective, safe and sustainable catalysis. While today the majority of applications are in the pharmaceutical sector, new opportunities are arising every day in other industry sectors, where production costs become a more important driver. In the early applications of the technology, it was necessary to design processes to match the properties of the biocatalyst. With the advent of protein engineering, organic chemists started to develop and improve enzymes to suit their needs...
January 13, 2018: Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences
Uwe T Bornscheuer
Biocatalysis has undergone a tremendous development in the past few years. A plethora of methods enable the rather rapid tailored-design of an enzyme for a targeted reaction such as asymmetric synthesis of a chiral building block by the combination of information from sequence and structure databases with modern molecular biology methods and high-throughput screening tools. Moreover, novel non-natural reactions could be implemented into protein scaffolds and new enzyme classes are emerging, both broadening the repertoire of reactions now available for organic synthesis...
January 13, 2018: Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences
Lei Han, Yukun Zhao, Shan Cui, Bo Liang
Microbial cell surface display technology can redesign cell surfaces with functional proteins and peptides to endow cells some unique features. Foreign peptides or proteins are transported out of cells and immobilized on cell surface by fusing with anchoring proteins, which is an effective solution to avoid substance transfer limitation, enzyme purification, and enzyme instability. As the most frequently used prokaryotic and eukaryotic protein surface display system, bacterial and yeast surface display systems have been widely applied in vaccine, biocatalysis, biosensor, bioadsorption, and polypeptide library screening...
November 22, 2017: Applied Biochemistry and Biotechnology
Jason Carere, Yousef I Hassan, Dion Lepp, Ting Zhou
The biological detoxification of mycotoxins, including deoxynivalenol (DON), represents a very promising approach to address the challenging problem of cereal grain contamination. The recent discovery of Devosia mutans 17-2-E-8 (Devosia spp. 17-2-E-8), a bacterial isolate capable of transforming DON to the non-toxic stereoisomer 3-epi-deoxynivalenol, along with earlier reports of bacterial species capable of oxidizing DON to 3-keto-DON, has generated interest in the possible mechanism and enzyme(s) involved...
November 17, 2017: Microbial Biotechnology
Katharina Schmölzer, Martin Lemmerer, Bernd Nidetzky
Glycosyltransferase cascades are promising tools of biocatalysis for natural product glycosylation, but their suitability for actual production remains to be shown. Here, we demonstrate at a scale of 100 g isolated product the integrated biocatalytic production of nothofagin, the natural 3'-C-β-D-glucoside of the polyphenol phloretin. A parallel reaction cascade involving coupled C-glucosyltransferase and sucrose synthase was optimized for the one-pot glucosylation of phloretin from sucrose via an UDP/UDP-glucose shuttle...
November 13, 2017: Biotechnology and Bioengineering
Long Chen, Megan Holmes, Elise Schaefer, Ashok Mulchandani, Xin Ge
We report a spore-based biocatalysis platform capable of producing and self-assembling active multimeric enzymes on a spore surface with a high loading density. This was achieved by co-expressing both a spore surface-anchoring scaffoldin protein containing multiple cohesin domains and a dockerin-tagged enzyme of interest in the mother cell compartment during Bacillus subtilis sporulation. Using this method, tetrameric β-galactosidase was successfully displayed on the spore surface with a loading density of 1...
November 13, 2017: Biotechnology and Bioengineering
Jian-Wei Zhao, Hua-Lei Wu, Jian-Dong Zhang, Wen-Chao Gao, Xiao-Jun Fan, Hong-Hong Chang, Wen-Long Wei, Jian-He Xu
OBJECTIVES: To investigate the efficiency of a new cascade biocatalysis system for the conversion of R, S-β-amino alcohols to enantiopure vicinal diol and β-amino alcohol. RESULTS: An efficient cascade biocatalysis was achieved by combination of a transaminase, a carbonyl reductase and a cofactor regeneration system. An ee value of > 99% for 2-amino-2-phenylethanol and 1-phenyl-1, 2-ethanediol were simultaneously obtained with 50% conversion from R, S-2-amino-2-phenylethanol...
November 9, 2017: Biotechnology Letters
Fei Chang, Xianbing Zhang, Yu Pan, Youxue Lu, Wei Fang, Zemin Fang, Yazhong Xiao
BACKGROUND: β-Glucosidase has attracted substantial attention in the scientific community because of its pivotal role in cellulose degradation, glycoside transformation and many other industrial processes. However, the tedious and costly expression and purification procedures have severely thwarted the industrial applications of β-glucosidase. Thus development of new strategies to express β-glucosidases with cost-effective and simple procedure to meet the increasing demands on enzymes for biocatalysis is of paramount importance...
November 7, 2017: BMC Biotechnology
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