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Biocatalysis

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https://www.readbyqxmd.com/read/28816042/rapid-and-reliable-detection-of-alkaline-phosphatase-by-a-hot-spots-amplification-strategy-based-on-well-controlled-assembly-on-single-nanoparticle
#1
Yi Zeng, Jia-Qiang Ren, Shaokai Wang, Jiaming Mai, Bing Qu, Yan Zhang, Ai-Guo Shen, Jiming Hu
The first appeal of clinical assay is always accurate and rapid. For alkaline phosphatase (ALP) monitoring in medical treatment, a rapid, reliable surface-enhanced Raman scattering (SERS) test kit is designed based on a "hot spots" amplification strategy. Consisting of alkyne-tagged Au nanoparticles (NPs), Ag+, and enzyme substrate, the packaged test kit can achieve one-step clinical assay of ALP in human serum within several minutes, while the operation is simple as it directly inputs the sample into the test kit...
August 17, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28813686/genetic-and-functional-characterization-of-a-novel-gh10-endo-%C3%AE-1-4-xylanase-with-a-ricin-type-%C3%AE-trefoil-domain-like-domain-from-luteimicrobium-xylanilyticum-hy-24
#2
Do Young Kim, Sun Hwa Lee, Min Ji Lee, Han-Young Cho, Jong Suk Lee, Young Ha Rhee, Dong-Ha Shin, Kwang-Hee Son, Ho-Yong Park
The gene (1488-bp) encoding a novel GH10 endo-β-1,4-xylanase (XylM) consisting of an N-terminal catalytic GH10 domain and a C-terminal ricin-type β-trefoil lectin domain-like (RICIN) domain was identified from Luteimicrobium xylanilyticum HY-24. The GH10 domain of XylM was 72% identical to that of Micromonospora lupini endo-β-1,4-xylanase and the RICIN domain was 67% identical to that of Actinospica robiniae hypothetical protein. The recombinant enzyme (rXylM: 49kDa) exhibited maximum activity toward beechwood xylan at 65°C and pH 6...
August 13, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28809952/computational-and-experimental-analysis-of-short-peptide-motifs-for-enzyme-inhibition
#3
Jinglin Fu, Luca Larini, Anthony J Cooper, John W Whittaker, Azka Ahmed, Junhao Dong, Minyoung Lee, Ting Zhang
The metabolism of living systems involves many enzymes that play key roles as catalysts and are essential to biological function. Searching ligands with the ability to modulate enzyme activities is central to diagnosis and therapeutics. Peptides represent a promising class of potential enzyme modulators due to the large chemical diversity, and well-established methods for library synthesis. Peptides and their derivatives are found to play critical roles in modulating enzymes and mediating cellular uptakes, which are increasingly valuable in therapeutics...
2017: PloS One
https://www.readbyqxmd.com/read/28803132/quantum-chemical-study-of-mechanism-and-stereoselectivity-of-secondary-alcohol-dehydrogenase
#4
Sara Moa, Fahmi Himo
Secondary alcohol dehydrogenase from Thermoanaerobacter brockii (TbSADH) is a Zn- and NADP-dependent enzyme that catalyses the reversible transformation of secondary alcohols into ketones. It is of potential biocatalytic interest as it can be used in the synthesis of chiral alcohols by asymmetric reduction of ketones. In this paper, density functional theory calculations are employed to elucidate the origins of the enantioselectivity of TbSADH using a large model of the active site and considering two different substrates, 2-butanol and 3-hexanol...
July 24, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28797956/orientation-and-density-control-of-proteins-on-solid-matters-by-outer-membrane-coating-analytical-and-diagnostic-applications
#5
REVIEW
Min Park, Jae-Chul Pyun, Joachim Jose
Autodisplay is an expression system for the display of recombinant proteins on the outer membrane (OM) of gram negative bacteria and has been developed for translocation studies, whole cell biocatalysis, bioremediation, inhibitor screening, and enzyme refolding. Recently, affinity proteins such as IgG-binding Z-domains and biotin-binding streptavidin have been autodisplayed on the OM of Escherichia coli for analytical and biomedical applications. The secretion mechanism of the autodisplay system was used and orientation and density control of these affinity proteins were determined...
July 31, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28797226/network-design-and-analysis-for-multi-enzyme-biocatalysis
#6
Lisa Katharina Blaß, Christian Weyler, Elmar Heinzle
BACKGROUND: As more and more biological reaction data become available, the full exploration of the enzymatic potential for the synthesis of valuable products opens up exciting new opportunities but is becoming increasingly complex. The manual design of multi-step biosynthesis routes involving enzymes from different organisms is very challenging. To harness the full enzymatic potential, we developed a computational tool for the directed design of biosynthetic production pathways for multi-step catalysis with in vitro enzyme cascades, cell hydrolysates and permeabilized cells...
August 10, 2017: BMC Bioinformatics
https://www.readbyqxmd.com/read/28795176/h2-driven-biocatalytic-hydrogenation-in-continuous-flow-using-enzyme-modified-carbon-nanotube-columns
#7
Ceren Zor, Holly A Reeve, Jonathan Quinson, Lisa A Thompson, Thomas H Lonsdale, Frank Dillon, Nicole Grobert, Kylie A Vincent
We describe the implementation of a system of immobilised enzymes for H2-driven NADH recycling coupled to a selective biotransformation to enable H2-driven biocatalysis in flow. This approach represents a platform that can be optimised for a wide range of hydrogenation steps and is shown here for enantioselective ketone reduction and reductive amination.
August 10, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28794956/recent-progress-on-deep-eutectic-solvents-in-biocatalysis
#8
REVIEW
Pei Xu, Gao-Wei Zheng, Min-Hua Zong, Ning Li, Wen-Yong Lou
Deep eutectic solvents (DESs) are eutectic mixtures of salts and hydrogen bond donors with melting points low enough to be used as solvents. DESs have proved to be a good alternative to traditional organic solvents and ionic liquids (ILs) in many biocatalytic processes. Apart from the benign characteristics similar to those of ILs (e.g., low volatility, low inflammability and low melting point), DESs have their unique merits of easy preparation and low cost owing to their renewable and available raw materials...
2017: Bioresources and Bioprocessing
https://www.readbyqxmd.com/read/28793235/-r-r-butane-2-3-diol-dehydrogenase-from-bacillus-clausii-dsm-8716-t-cloning-and-expression-of-the-bdha-gene-and-initial-characterization-of-enzyme
#9
Lukas Muschallik, Denise Molinnus, Johannes Bongaerts, Martina Pohl, Torsten Wagner, Michael J Schöning, Petra Siegert, Thorsten Selmer
The gene encoding a putative (R,R)-butane-2,3-diol dehydrogenase (bdhA) from Bacillus clausii DSM 8716(T) was isolated, sequenced and expressed in Escherichia coli. The amino acid sequence of the encoded protein is only distantly related to previously studied enzymes (identity 33-43%) and exhibited some uncharted peculiarities. An N-terminally StrepII-tagged enzyme variant was purified and initially characterized. The isolated enzyme catalyzed the (R)-specific oxidation of (R,R)- and meso-butane-2,3-diol to (R)- and (S)-acetoin with specific activities of 12U/mg and 23U/mg, respectively...
August 6, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28762640/exploring-bacterial-carboxylate-reductases-for-the-reduction-of-bifunctional-carboxylic-acids
#10
Anna N Khusnutdinova, Robert Flick, Ana Popovic, Greg Brown, Anatoli Tchigvintsev, Boguslaw Nocek, Kevin Correia, Jeong Chan Joo, Radhakrishnan Mahadevan, Alexander F Yakunin
Carboxylic acid reductases (CARs) selectively reduce carboxylic acids to aldehydes using ATP and NADPH as cofactors under mild conditions. Although CARs have attracted significant interest, only a few enzymes have been characterized to date, whereas the vast majority of CARs have yet to be examined. Herein we report that 12 bacterial CARs reduced a broad range of bifunctional carboxylic acids containing oxo-, hydroxy-, amino-, or second carboxyl groups with several enzymes showing activity toward 4-hydroxybutanoic (4-HB) and adipic acids...
August 1, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28755609/a-resettable-and-reprogrammable-keypad-lock-based-on-electrochromic-prussian-blue-films-and-biocatalysis-of-immobilized-glucose-oxidase-in-a-bipolar-electrode-system
#11
Xue Yu, Jiying Liang, Tiangang Yang, Mengjie Gong, Dongman Xi, Hongyun Liu
Herein, a resettable and reprogrammable biomolecular keypad lock on the basis of a closed bipolar electrode (BPE) system was established. In this system, one ITO electrode with immobilized chitosan (CS) and glucose oxidase (GOD), designated as CS-GOD, acted as one pole of BPE in the sensing cell; another ITO with electrodeposited Prussian blue (PB) films as the other pole in the reporting cell. The addition of ascorbic acid (AA) in the sensing cell with driving voltage (Vtot) at +2.5V would make the PB films become Prussian white (PW) in the reporting cell, accompanied by the color change from blue to nearly transparent...
July 22, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28754115/in-vivo-plug-and-play-a-modular-multi-enzyme-single-cell-catalyst-for-the-asymmetric-amination-of-ketoacids-and-ketones
#12
Judith E Farnberger, Elisabeth Lorenz, Nina Richter, Volker F Wendisch, Wolfgang Kroutil
BACKGROUND: Transaminases have become a key tool in biocatalysis to introduce the amine functionality into a range of molecules like prochiral α-ketoacids and ketones. However, due to the necessity of shifting the equilibrium towards the product side (depending on the amine donor) an efficient amination system may require three enzymes. So far, this well-established transformation has mainly been performed in vitro by assembling all biocatalysts individually, which comes along with elaborate and costly preparation steps...
July 28, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28745876/ionic-liquids-as-tool-to-improve-enzymatic-organic-synthesis
#13
REVIEW
Toshiyuki Itoh
Ionic liquids (ILs) have now been acknowledged as reaction media for biotransformations. The first three examples were reported in this field in 2000, and since then, numerous applications have been reported for biocatalytic reactions using ILs. Two topics using ILs for enzymatic reactions have been reviewed from the standpoint of biocatalyst mediating organic synthesis; the first is "Biocatalysis in Ionic Liquids" which includes various types of biocatalytic reactions in ILs (section 2): (1) recent examples of lipase-mediated reactions using ILs as reaction media for biodiesel oil production and for sugar ester production, (2) oxidase-catalyzed reactions in ILs, (3) laccase-catalyzed reactions, (4) peroxidase-catalyzed reactions, (4) cytochrome-mediated reactions, (5) microbe-mediated hydrations, (6) protease-catalyzed reactions, (8) whole cell mediated asymmetric reduction of ketones, (10) acylase-catalyzed reactions, (11) glycosylation or cellulase-mediated hydrolysis of polysaccharides, (12) hydroxynitrile lyase-catalyzed reaction, (13) fluorinase or haloalkane dehydrogenase-catalyzed reaction, (14) luciferase-catalyzed reactions, and (15) biocatalytic promiscuity of enzymatic reactions for organic synthesis using ILs...
August 9, 2017: Chemical Reviews
https://www.readbyqxmd.com/read/28743564/evolving-biocatalysis-to-meet-bioeconomy-challenges-and-opportunities
#14
REVIEW
Alessandro Pellis, Sara Cantone, Cynthia Ebert, Lucia Gardossi
The unique selectivity of enzymes, along with their remarkable catalytic activity, constitute powerful tools for transforming renewable feedstock and also for adding value to an array of building blocks and monomers produced by the emerging bio-based chemistry sector. Although some relevant biotransformations run at the ton scale demonstrate the success of biocatalysis in industry, there is still a huge untapped potential of catalytic activities available for targeted valorization of new raw materials, such as waste streams and CO2...
July 22, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28742253/precisely-designed-isopeptide-bridge-crosslinking-endows-artificial-hydrolases-with-predominant-stability-and-catalytic-activity-under-extreme-denaturing-conditions
#15
Yu Bai, Chao Wang, Guodong Liang, Wenqing Lai, Huifang Xue, Yanbo Ling, Maosheng Cheng, Keliang Liu
Enzymes normally lose their activities under extreme conditions due to the dissociation of their active tertiary structure. If an enzyme could maintain its catalytic activity under non physiological or denaturing conditions, it might be used in more applications in the pharmaceutical and chemical industries. Recently, we reported a coiled-coil six-helical bundle (6HB) structure as a scaffold for designing artificial hydrolytic enzymes. Here, intermolecular isopeptide bonds were incorporated to enhance the stability and activity of such biomolecules under denaturing conditions...
July 25, 2017: Chemistry, An Asian Journal
https://www.readbyqxmd.com/read/28741462/development-of-a-solid-phase-array-assay-for-the-screening-of-galactose-oxidase-activity-and-for-fast-identification-of-inhibitors
#16
Martin J Weissenborn, Damien P Debecker, Bruno Linclau, Nicolas J Turner, Sabine Flitsch
Galactose oxidase catalyses the highly selective oxidation of terminal galactosides on a wide range of natural glycoconjugates and has found wide applications in biotechnology, particularly in biocatalysis. Here we show that the enzyme is able to oxidise solid gold-SAM surfaces functionalised with galactose. This array platform allows for the fast identification of galactose oxidase substrates and inhibitors from a library of deoxy-fluorinated sugars using MALDI-Tof spectrometry as a label -free read-out method...
July 24, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28738232/molecular-tools-and-emerging-strategies-for-deep-genetic-genomic-refactoring-of-pseudomonas
#17
REVIEW
Esteban Martínez-García, Víctor de Lorenzo
The interest of the genus Pseudomonas largely relies on the virulence of some of its species for plants and animals (including humans). Yet, pathogenic features of some isolates coexist with others often present in environmental variants that promote plant growth and degrade chemical pollutants. Many of these traits can be traced to the intrinsic properties of the genomic chassis of this genus along with distinct genetic parts and devices. With the tools of Synthetic Biology these can be enhanced and/or repurposed for the sake of biological control, environmental remediation and whole-cell biocatalysis...
July 21, 2017: Current Opinion in Biotechnology
https://www.readbyqxmd.com/read/28732194/principles-of-chemical-biology-apex-cell-free-enzyme-systems-new-antibiotics-epigenetic-membrane-relationship-and-metastable-transcription
#18
(no author information available yet)
This month: GPCR signaling and networks in APEX, making terpenes via biocatalysis in a cell-free system, antibiotics for Gram-negative bugs, the role of membranes in epigenetics, and the role of metastability in transcription.
July 20, 2017: Cell Chemical Biology
https://www.readbyqxmd.com/read/28731627/his-tag-immobilization-of-cutinase-1-from-thermobifida-cellulosilytica-for-solvent-free-synthesis-of-polyesters
#19
Alessandro Pellis, Marco Vastano, Felice Quartinello, Enrique Herrero Acero, Georg M Guebitz
For many years, lipase B from Candida antarctica (CaLB) was the primary biocatalyst used for enzymatic esterification and polycondensation reactions. More recently, the need for novel biocatalysts with different selectivity has arisen in the biotechnology and biocatalysis fields. The present work describes how the catalytic potential of Thermobifida cellulosilytica cutinase 1 (Thc_Cut1) was exploited for polyester synthesis. in a first step, Thc_Cut1 was immobilized on three different carriers, namely Opal, Coral and Amber, using a novel non-toxic His-tag method based on chelated Fe(iii) ions (>99% protein bounded)...
July 21, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28717785/the-effects-of-high-concentrations-of-ionic-liquid-on-gb1-protein-structure-and-dynamics-probed-by-high-resolution-magic-angle-spinning-nmr-spectroscopy
#20
Lisa Warner, Erica Gjersing, Shelby E Follett, K Wade Elliott, Sergei V Dzyuba, Krisztina Varga
Ionic liquids have great potential in biological applications and biocatalysis, as some ionic liquids can stabilize proteins and enhance enzyme activity, while others have the opposite effect. However, on the molecular level, probing ionic liquid interactions with proteins, especially in solutions containing high concentration of ionic liquids, has been challenging. In the present work the (13)C, (15)N-enriched GB1 model protein was used to demonstrate applicability of high-resolution magic-angle-spinning (HR-MAS) NMR spectroscopy to investigate ionic liquid - protein interactions...
December 2016: Biochemistry and Biophysics Reports
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