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https://www.readbyqxmd.com/read/28092858/the-synthesis-and-antistaphylococcal-activity-of-9-13-disubstituted-berberine-derivatives
#1
Jing Wang, Teng Yang, Huang Chen, Yun-Nan Xu, Li-Fang Yu, Ting Liu, Jie Tang, Zhengfang Yi, Cai-Guang Yang, Wei Xue, Fan Yang
A series of novel 9, 13-disubstituted berberine derivatives have been synthesized and evaluated for the antibacterial activities against Staphylococcus aureus, including Newman strain and multidrug-resistant strains (NRS-1, NRS-70, NRS-100, NRS-108, and NRS-271). Compound 20 shows the most potent activity against the growth of Newman strain, with a MIC value of 0.78 μg/mL, which is comparable with the positive control vancomycin. In addition, compound 20, 21, and 33 are highly antistaphylococcal active against five strains of multidrug-resistant S...
January 9, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28092646/ex-vivo-nonviral-gene-delivery-of-%C3%AE-opioid-receptor-to-attenuate-cancer-induced-pain
#2
Seiichi Yamano, Chi T Viet, Dongmin Dang, Jisen Dai, Shigeru Hanatani, Tadahiro Takayama, Hironori Kasai, Kentaro Imamura, Ron Campbell, Yi Ye, John C Dolan, William Myung Kwon, Stefan D Schneider, Brian L Schmidt
Virus-mediated gene delivery shows promise for the treatment of chronic pain. However, viral vectors have cytotoxicity. To avoid toxicities and limitations of virus-mediated gene delivery, we developed a novel nonviral hybrid vector: HIV-1 Tat peptide sequence modified with histidine and cysteine residues combined with a cationic lipid. The vector has high transfection efficiency with little cytotoxicity in cancer cell lines including HSC-3 (human tongue squamous cell carcinoma) and exhibits differential expression in HSC-3 (∼45-fold) relative to HGF-1 (human gingival fibroblasts) cells...
February 2017: Pain
https://www.readbyqxmd.com/read/28092351/pulmonary-complications-post-hematopoietic-stem-cell-transplant-in-dyskeratosis-congenita-analysis-of-oxidative-stress-in-lung-fibroblasts
#3
C Sorge, L Pereboeva, E Westin, W T Harris, D R Kelly, F Goldman
No abstract text is available yet for this article.
January 16, 2017: Bone Marrow Transplantation
https://www.readbyqxmd.com/read/28092083/role-of-dopamine-d2-d3-receptors-in-development-plasticity-and-neuroprotection-in-human-ipsc-derived-midbrain-dopaminergic-neurons
#4
Federica Bono, Paola Savoia, Adele Guglielmi, Massimo Gennarelli, Giovanna Piovani, Sandra Sigala, Damiana Leo, Stefano Espinoza, Raul R Gainetdinov, Paola Devoto, PierFranco Spano, Cristina Missale, Chiara Fiorentini
The role of dopamine D2 and D3 receptors (D2R/D3R), located on midbrain dopaminergic (DA) neurons, in the regulation of DA synthesis and release and in DA neuron homeostasis has been extensively investigated in rodent animal models. By contrast, the properties of D2R/D3R in human DA neurons have not been elucidated yet. On this line, the use of human-induced pluripotent stem cells (hiPSCs) for producing any types of cells has offered the innovative opportunity for investigating the human neuronal phenotypes at the molecular levels...
January 14, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28091894/mechanisms-of-action-of-surgical-interventions-on-weight-related-diseases-the-potential-role-of-bile-acids
#5
REVIEW
Mohsen Mazidi, Pedro Paulo P de Caravatto, John R Speakman, Ricardo V Cohen
Surgical interventions for weight-related diseases (SWRD) may have substantial and sustainable effect on weight reduction, also leading to a higher remission rate of type 2 diabetes (T2D) mellitus than any other medical treatment or lifestyle intervention. The resolution of T2D after Roux-en-Y gastric bypass (RYGB) typically occurs too quickly to be accounted for by weight loss alone, suggesting that these operations have a direct impact on glucose homeostasis. The mechanisms underlying these beneficial effects however remain unclear...
January 14, 2017: Obesity Surgery
https://www.readbyqxmd.com/read/28091697/heat-shock-transcription-factor-1-protects-against-pressure-overload-induced-cardiac-fibrosis-via-smad3
#6
Ning Zhou, Yong Ye, Xingxu Wang, Ben Ma, Jian Wu, Lei Li, Lin Wang, Dao Wen Wang, Yunzeng Zou
: Fibrotic cardiac muscle exhibits high stiffness and low compliance which are major risk factors of heart failure. Although heat shock transcription factor 1 (HSF1) was identified as an intrinsic cardioprotective factor, the role that HSF1 plays in cardiac fibrosis remains unclear. Our study aims to investigate the role of HSF1 in pressure overload-induced cardiac fibrosis and the underlying mechanism. HSF1 phosphorylation was significantly downregulated in transverse aortic constriction (TAC)-treated mouse hearts and mechanically stretched cardiac fibroblasts (cFBs)...
January 13, 2017: Journal of Molecular Medicine: Official Organ of the "Gesellschaft Deutscher Naturforscher und Ärzte"
https://www.readbyqxmd.com/read/28091585/microrna-19b-is-a-potential-biomarker-of-increased-myocardial-collagen-cross-linking-in-patients-with-aortic-stenosis-and-heart-failure
#7
Javier Beaumont, Begoña López, Susana Ravassa, Nerea Hermida, Gorka San José, Idoia Gallego, Félix Valencia, Juan José Gómez-Doblas, Eduardo de Teresa, Javier Díez, Arantxa González
This study analyzed the potential associations of 7 myocardial fibrosis-related microRNAs with the quality of the collagen network (e.g., the degree of collagen fibril cross-linking or CCL) and the enzyme lysyl oxidase (LOX) responsible for CCL in 28 patients with severe aortic stenosis (AS) of whom 46% had a diagnosis of chronic heart failure (HF). MicroRNA expression was analyzed in myocardial and blood samples. From the studied microRNAs only miR-19b presented a direct correlation (p < 0.05) between serum and myocardium...
January 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28091523/origin-of-anti-tumor-activity-of-the-cysteine-containing-go-peptides-and-further-optimization-of-their-cytotoxic-properties
#8
Irina I Tyuryaeva, Olga G Lyublinskaya, Ivan S Podkorytov, Nikolai R Skrynnikov
Antitumor GO peptides have been designed as dimerization inhibitors of prominent oncoprotein mucin 1. In this study we demonstrate that activity of GO peptides is independent of the level of cellular expression of mucin 1. Furthermore, these peptides prove to be broadly cytotoxic, causing cell death also in normal cells such as dermal fibroblasts and endometrial mesenchymal stem cells. To explore molecular mechanism of their cytotoxicity, we have designed and tested a number of new peptide sequences containing the key CxC or CxxC motifs...
January 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28090808/biomaterial-encapsulation-is-enhanced-in-the-early-stages-of-the-foreign-body-reaction-during-conditional-macrophage-depletion-in-transgenic-mafia-mice
#9
Ruud A Bank, Jurjen Zandstra, Hilde Room, Arjen H Petersen, Sander Maarten van Putten
Macrophages are pivotal cells during the foreign body reaction, as they orchestrate the pro-inflammatory microenvironment inside and around biomaterials by secretion of inflammatory mediators. Furthermore they are responsible for the degradation of biomaterials and are thought to instruct the fibroblasts that generate a fibrous capsule around implanted biomaterials. In this study we investigated the events during the FBR when macrophages are not present. Hexamethylenediisocyanate cross-linked collagen scaffolds were implanted in "Macrophage Fas-Induced Apoptosis" (MaFIA) mice, which allow "on demand" macrophage depletion...
January 15, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28090803/insulin-like-growth-factor-binding-protein-2-in-bipolar-disorder-an-expression-study-in-peripheral-tissues
#10
Elena Milanesi, Roberta Zanardini, Gianluca Rosso, Giuseppe Maina, Alessandro Barbon, Cristina Mora, Alessandra Minelli, Massimo Gennarelli, Luisella Bocchio-Chiavetto
OBJECTIVES: Insulin-like Growth Factor Binding Protein 2 (IGFBP2) is a member of the family of high-affinity binding proteins (IGFBP1-6) and appears to play a governing role in Insulin-like Growth Factor (IGF) regulation in the central nervous system. This study aimed to investigate the putative involvement of IGFBP2 in mood disorder pathogenesis by measuring its expression levels in patient peripheral tissues. METHODS: IGFBP2 protein and mRNA levels were measured in the serum of 93 controls, 41 Bipolar Disorder (BD) and 43 Major Depressive Disorder (MDD) patients and in the skin fibroblasts from 15 controls, 12 BD and 23 MDD patients...
January 16, 2017: World Journal of Biological Psychiatry
https://www.readbyqxmd.com/read/28090771/differential-adhesive-bioactive-properties-of-polymeric-surface-coated-with-graphene-oxide-thin-film
#11
Sudhin Thampi, A Maya Nandkumar, Vignesh Muthuvijayan, Ramesh Parameswaran
Surface engineering of implantable devices involving polymeric biomaterials has become an essential aspect for medical implants. Surface enhancement technique can provide an array of unique surface properties that improves its biocompatibility and functionality as an implant. Polyurethane based implants that have found extensively acclaimed usage as an implant in biomedical applications, especially in the area of cardiovascular devices still lacks any mechanism to ward off bacterial or platelet adhesion. To bring out such a defense mechanism we are proposing a surface modification technique...
January 16, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28090290/shared-gene-expression-patterns-in-mesenchymal-progenitors-derived-from-lung-and-epidermis-in-pulmonary-arterial-hypertension-identifying-key-pathways-in-pulmonary-vascular-disease
#12
Christa Gaskill, Shennea Marriott, Sidd Pratap, Swapna Menon, Lora K Hedges, Joshua P Fessel, Jonathan A Kropski, DeWayne Ames, Lisa Wheeler, James E Loyd, Anna R Hemnes, Dennis R Roop, Dwight J Klemm, Eric D Austin, Susan M Majka
Rapid access to lung-derived cells from stable subjects is a major challenge in the pulmonary hypertension field, given the relative contraindication of lung biopsy. In these studies, we sought to demonstrate the importance of evaluating a cell type that actively participates in disease processes, as well as the potential to translate these findings to vascular beds in other nonlung tissues, in this instance perivascular skin mesenchymal cells (MCs). We utilized posttransplant or autopsy lung explant-derived cells (ABCG2-expressing mesenchymal progenitor cells [MPCs], fibroblasts) and skin-derived MCs to test the hypothesis that perivascular ABCG2 MPCs derived from pulmonary arterial hypertension (PAH) patient lung and skin would express a gene profile reflective of ongoing vascular dysfunction...
December 2016: Pulmonary Circulation
https://www.readbyqxmd.com/read/28090093/upar-promotes-tumor-like-biologic-behaviors-of-fibroblast-like-synoviocytes-through-pi3k-akt-signaling-pathway-in-patients-with-rheumatoid-arthritis
#13
Yan Liu, Yun Feng Pan, You-Qiu Xue, Lin-Kai Fang, Xing-Hua Guo, Xin Guo, Meng Liu, Bi-Yao Mo, Meng-Ru Yang, Fang Liu, Yun-Ting Wu, Nancy Olsen, Song Guo Zheng
Urokinase-type plasminogen activator receptor (uPAR), is a multifunctional receptor on cell surface, widely present in endothelial cells, fibroblasts, and a variety of malignant cells. Current studies have suggested that uPAR overexpressed on synovial tissues or in synovial fluid or plasma in patients with rheumatoid arthritis (RA). However, there are limited researches regarding the role of uPAR on fibroblast-like synoviocytes of rheumatoid arthritis (RA-FLSs) and its underlying mechanisms. Here, our studies show that the expression of uPAR protein was significantly higher in fibroblast-like synoviocytes (FLSs) from RA than those from osteoarthritis or traumatic injury patients...
January 16, 2017: Cellular & Molecular Immunology
https://www.readbyqxmd.com/read/28089995/systematic-evaluation-of-markers-used-for-the-identification-of-human-induced-pluripotent-stem-cells
#14
Sumitha Prameela Bharathan, Kannan Vrindavan Manian, Syed Mohammed Musheer Aalam, Dhavapriya Palani, Prashant Ajit Deshpande, Mankuzhy Damodaran Pratheesh, Alok Srivastava, Shaji Ramachandran Velayudhan
Low efficiency of somatic cell reprogramming and heterogeneity among human induced pluripotent stem cells (hiPSCs) demand extensive characterization of isolated clones before their use in downstream applications. By monitoring human fibroblasts undergoing reprogramming for their morphological changes and expression of fibroblast (CD13), pluripotency markers (SSEA-4 and TRA-1-60) and a retrovirally expressed red fluorescent protein (RV-RFP), we compared the efficiency of these features to identify bona fide hiPSC colonies...
January 15, 2017: Biology Open
https://www.readbyqxmd.com/read/28089671/pdgfr%C3%AE-cells-in-embryonic-stem-cell-cultures-represent-the-in%C3%A2-vitro-equivalent-of-the-pre-implantation-primitive-endoderm-precursors
#15
Antonio Lo Nigro, Anchel de Jaime-Soguero, Rita Khoueiry, Dong Seong Cho, Giorgia Maria Ferlazzo, Ilaria Perini, Vanesa Abon Escalona, Xabier Lopez Aranguren, Susana M Chuva de Sousa Lopes, Kian Peng Koh, Pier Giulio Conaldi, Wei-Shou Hu, An Zwijsen, Frederic Lluis, Catherine M Verfaillie
In early mouse pre-implantation development, primitive endoderm (PrE) precursors are platelet-derived growth factor receptor alpha (PDGFRα) positive. Here, we demonstrated that cultured mouse embryonic stem cells (mESCs) express PDGFRα heterogeneously, fluctuating between a PDGFRα+ (PrE-primed) and a platelet endothelial cell adhesion molecule 1 (PECAM1)-positive state (epiblast-primed). The two surface markers can be co-detected on a third subpopulation, expressing epiblast and PrE determinants (double-positive)...
January 5, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28089670/in%C3%A2-vitro-propagation-and-branching-morphogenesis-from-single-ureteric-bud%C3%A2-cells
#16
Shunsuke Yuri, Masaki Nishikawa, Naomi Yanagawa, Oak D Jo, Norimoto Yanagawa
A method to maintain and rebuild ureteric bud (UB)-like structures from UB cells in vitro could provide a useful tool for kidney regeneration. We aimed in our present study to establish a serum-free culture system that enables the expansion of UB progenitor cells, i.e., UB tip cells, and reconstruction of UB-like structures. We found that fibroblast growth factors or retinoic acid (RA) was sufficient for the survival of UB cells in serum-free condition, while the proliferation and maintenance of UB tip cells required glial cell-derived neurotrophic factor together with signaling from either WNT-β-catenin pathway or RA...
January 5, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28089496/cell-toxicity-in-fibroblasts-tenocytes-and-human-mesenchymal-stem-cells-a-comparison-of-necrosis-and-apoptosis-inducing-ability-in-ropivacaine-bupivacaine-and-triamcinolone
#17
Anja Z Zhang, Andreas Ficklscherer, Mehmet F Gülecyüz, Alexander C Paulus, Thomas R Niethammer, Volkmar Jansson, Peter E Müller
PURPOSE: To analyze the ability of ropivacaine, bupivacaine, and triamcinolone to induce apoptosis and necrosis in fibroblasts, tenocytes, and human mesenchymal stem cells. METHODS: Human dermal fibroblasts, adipose-derived human mesenchymal stem cells (hMSCs), and tenocytes gained from the rotator cuff tendon were seeded with a cell density of 0.5 × 10(4)/cm(2). One specimen of ropivacaine, bupivacaine, and triamcinolone was tested separately on the cells with separate concentrations of 0...
January 12, 2017: Arthroscopy: the Journal of Arthroscopic & related Surgery
https://www.readbyqxmd.com/read/28089450/a-structural-model-for-vinculin-insertion-into-pip2-containing-membranes-and-the-effect-of-insertion-on-vinculin-activation-and-localization
#18
Peter M Thompson, Srinivas Ramachandran, Lindsay B Case, Caitlin E Tolbert, Arpit Tandon, Mihir Pershad, Nikolay V Dokholyan, Clare M Waterman, Sharon L Campbell
Vinculin, a scaffolding protein that localizes to focal adhesions (FAs) and adherens junctions, links the actin cytoskeleton to the adhesive super-structure. While vinculin binds to a number of cytoskeletal proteins, it can also associate with phosphatidylinositol 4,5-bisphosphate (PIP2) to drive membrane association. To generate a structural model for PIP2-dependent interaction of vinculin with the lipid bilayer, we conducted lipid-association, nuclear magnetic resonance, and computational modeling experiments...
December 31, 2016: Structure
https://www.readbyqxmd.com/read/28089391/camsap3-dependent-microtubule-dynamics-regulates-golgi-assembly-in-epithelial-cells
#19
Jing Wang, Honglin Xu, Yuqiang Jiang, Mikiko Takahashi, Masatoshi Takeichi, Wenxiang Meng
The Golgi assembly pattern varies among cell types. In fibroblast cells, the Golgi apparatus concentrates around the centrosome that radiates microtubules; whereas in epithelial cells, whose microtubules are mainly noncentrosomal, the Golgi apparatus accumulates around the nucleus independently of centrosome. Little is known about the mechanisms behind such cell type-specific Golgi and microtubule organization. Here, we show that the microtubule minus-end binding protein Nezha/CAMSAP3 (calmodulin-regulated spectrin-associated protein 3) plays a role in translocation of Golgi vesicles in epithelial cells...
December 26, 2016: Journal of Genetics and Genomics, Yi Chuan Xue Bao
https://www.readbyqxmd.com/read/28089357/localization-of-toll-like-receptor-tlr-2-and-tlr4-mrna-in-the-colorectal-mucosa-of-miniature-dachshunds-with-inflammatory-colorectal-polyps
#20
N Yokoyama, H Ohta, J Yamazaki, Y Kagawa, O Ichii, N Khoirun, T Morita, T Osuga, S Y Lim, N Sasaki, K Morishita, K Nakamura, M Takiguchi
Inflammatory colorectal polyps (ICRPs) are characterized by the formation of multiple or solitary polyps with marked neutrophil infiltration in the colorectal area, and are speculated to be a novel form of breed-specific canine idiopathic inflammatory bowel disease (IBD). In human IBD, toll-like receptor (TLR) 2 and TLR4 have been reported to be involved in the pathogenesis of the disease. The aim of this study was to evaluate the expression of TLR2 and TLR4 mRNA in the colorectal mucosa of dogs with ICRPs by in-situ hybridization using an RNAscope assay...
January 12, 2017: Journal of Comparative Pathology
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