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https://www.readbyqxmd.com/read/28340475/irinotecan-upregulates-fibroblast-growth-factor-receptor-3-expression-in-colorectal-cancer-cells-which-mitigates-irinotecan-induced-apoptosis
#1
Zeynep N Erdem, Stefanie Schwarz, Daniel Drev, Christine Heinzle, Andrea Reti, Petra Heffeter, Xenia Hudec, Klaus Holzmann, Bettina Grasl-Kraupp, Walter Berger, Michael Grusch, Brigitte Marian
BACKGROUND: Irinotecan (IRI) is an integral part of colorectal cancer (CRC) therapy, but response rates are unsatisfactory and resistance mechanisms are still insufficiently understood. As fibroblast growth factor receptor 3 (FGFR3) mediates essential survival signals in CRC, it is a candidate gene for causing intrinsic resistance to IRI. METHODS: We have used cell line models overexpressing FGFR3 to study the receptor's impact on IRI response. For pathway blockade, a dominant-negative receptor mutant and a small molecule kinase inhibitor were employed...
March 21, 2017: Translational Oncology
https://www.readbyqxmd.com/read/28340249/orthodontic-strain-affects-the-hippo-pathway-effector-yap-concomitant-with-proliferation-in-human-periodontal-ligament-fibroblasts
#2
Diana Huelter-Hassler, Pascal Tomakidi, Thorsten Steinberg, Britta A Jung
Objectives: During orthodontic tooth movement (OTM), human periodontal ligament fibroblasts (hPDLFs) sense, and respond to mechanical forces. Since the molecular constituents involved in these processes are not fully elucidated, the objective of the present study was to identify further key molecules of the cellular strain response. Materials and Methods: Primary hPDLFs were strained with a static equiaxial strain of 2.5 per cent for 15 minutes, 1 hour, 6 hours, and 24 hours...
March 17, 2017: European Journal of Orthodontics
https://www.readbyqxmd.com/read/28339837/vitamin-d-treatment-attenuates-cardiac-fgf23-fgfr4-signaling-and-hypertrophy-in-uremic-rats
#3
Maren Leifheit-Nestler, Alexander Grabner, Laura Hermann, Beatrice Richter, Karin Schmitz, Dagmar-Christiane Fischer, Christopher Yanucil, Christian Faul, Dieter Haffner
Background.: Vitamin D deficiency and excess of circulating fibroblast growth factor 23 (FGF23) contribute to cardiovascular mortality in patients with chronic kidney disease (CKD). FGF23 activates FGF receptor 4 and (FGFR4) calcineurin/nuclear factor of activated T cells (NFAT) signaling in cardiac myocytes, thereby causing left ventricular hypertrophy (LVH). Here, we determined if 1,25-dihydroxyvitamin D (calcitriol) inhibits FGF23-induced cardiac signaling and LVH. Methods...
February 23, 2017: Nephrology, Dialysis, Transplantation
https://www.readbyqxmd.com/read/28339804/intra-cardiac-and-peripheral-levels-of-biochemical-markers-of-fibrosis-in-patients-undergoing-catheter-ablation-for-atrial-fibrillation
#4
Gordon A Begg, Rashed Karim, Tobias Oesterlein, Lee N Graham, Andrew J Hogarth, Stephen P Page, Christopher B Pepper, Kawal Rhode, Gregory Y H Lip, Arun V Holden, Sven Plein, Muzahir H Tayebjee
Aims: Measurement of circulating biomarkers of fibrosis may have a role in selecting patients and treatment strategy for catheter ablation. Pro-collagen type III N-terminal pro-peptide (PIIINP), C-telopeptide of type I collagen (ICTP), fibroblast growth factor 23 (FGF-23), and galectin 3 (gal-3) have all been suggested as possible biomarkers for this indication, but studies assessing whether peripheral levels reflect intra-cardiac levels are scarce. Methods and results: We studied 93 patients undergoing ablation for paroxysmal atrial fibrillation (AF) (n = 63) or non-paroxysmal AF (n = 30)...
February 27, 2017: Europace: European Pacing, Arrhythmias, and Cardiac Electrophysiology
https://www.readbyqxmd.com/read/28339537/stimulatory-effects-of-fibroblast-growth-factor-2-on-proliferation-and-migration-of-uterine-luminal-epithelial-cells-during-early-pregnancy%C3%A2-%C3%A2
#5
Whasun Lim, Hyocheol Bae, Fuller W Bazer, Gwonhwa Song
Fibroblast growth factor 2 (FGF2) is a mitogen that induces proliferation, differentiation, and migration of cells, as well as angiogenesis and carcinogenesis via autocrine or paracrine actions. Fibroblast growth factor 2 expression is abundant in porcine conceptuses and endometrium during the estrous cycle and peri-implantation period of pregnancy. However, its intracellular actions in uterine epithelial cells have not been reported. The results of this study indicated abundant expression of FGFR1 and FGFR2 predominantly in uterine luminal and glandular epithelia during early pregnancy and that their expression decreased with increasing parity of the sows...
December 20, 2016: Biology of Reproduction
https://www.readbyqxmd.com/read/28339479/mouse-newborn-cells-allow-highly-productive-mouse-cytomegalovirus-replication-constituting-a-novel-convenient-primary-cell-culture-system
#6
Vu Thuy Khanh Le-Trilling, Mirko Trilling
Mammalian cell culture is indispensable for most aspects of current biomedical research. Immortalized cell lines are very convenient, but transforming principles (e.g. oncogenic viruses or their oncogenes) can heavily influence the experimental outcome. Primary cells do not share this apparent disadvantage but are more laborious to generate. Certain viruses (e.g. mouse cytomegalovirus) do not replicate efficiently in most transformed cell lines. In the past, such viruses have been routinely propagated on primary mouse embryonic fibroblasts (MEF) established around day 17 (d17) of gestation...
2017: PloS One
https://www.readbyqxmd.com/read/28339457/targeted-aav5-smad7-gene-therapy-inhibits-corneal-scarring-in-vivo
#7
Suneel Gupta, Jason T Rodier, Ajay Sharma, Elizabeth A Giuliano, Prashant R Sinha, Nathan P Hesemann, Arkasubhra Ghosh, Rajiv R Mohan
Corneal scarring is due to aberrant activity of the transforming growth factor β (TGFβ) signaling pathway following traumatic, mechanical, infectious, or surgical injury. Altered TGFβ signaling cascade leads to downstream Smad (Suppressor of mothers against decapentaplegic) protein-mediated signaling events that regulate expression of extracellular matrix and myogenic proteins. These events lead to transdifferentiation of keratocytes into myofibroblasts through fibroblasts and often results in permanent corneal scarring...
2017: PloS One
https://www.readbyqxmd.com/read/28339089/hmgb1-induces-lung-fibroblast-to-myofibroblast-differentiation-through-nf%C3%A2-%C3%AE%C2%BAb%C3%A2-mediated-tgf%C3%A2-%C3%AE-1-release
#8
Qiong Wang, Jun Wang, Junfang Wang, Shanchao Hong, Feifei Han, Jingyu Chen, Guoqian Chen
The proinflammatory factor high‑mobility group box protein 1 (HMGB1) has been implicated in the pathogenesis of lung fibrosis; however, the role of HMGB1 in lung fibrosis remains unclear. It has previously been reported that nuclear factor (NF)‑κB and transforming growth factor (TGF)‑β1 may be involved in lung fibrosis. Therefore, the present study aimed to examine the potential molecular mechanisms that underlie HMGB1‑induced lung fibrosis via the regulation of NF‑κB and TGF‑β1. The results demonstrated that HMGB1 stimulation increased the activation of NF‑κB and the release of TGF‑β1, as well as the expression of α‑smooth muscle actin (α‑SMA) and collagen I in human lung fibroblasts in vitro...
March 23, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28339064/extracellular-matrix-metabolism-disorder-induced-by-mechanical-strain-on-human-parametrial-ligament-fibroblasts
#9
Jie Min, Bingshu Li, Cheng Liu, Wenjun Guo, Shasha Hong, Jianming Tang, Li Hong
Pelvic organ prolapse (POP) is a global health problem that may seriously impact the quality of life of the sufferer. The present study aimed to investigate the potential mechanisms underlying alterations in extracellular matrix (ECM) metabolism in the pathogenesis of POP, by investigating the expression of ECM components in human parametrial ligament fibroblasts (hPLFs) subject to various mechanical strain loads. Fibroblasts derived from parametrial ligaments were cultured from patients with POP and without malignant tumors, who underwent vaginal hysterectomy surgery...
March 24, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28339036/fgf7-fgfr2-signal-promotes-invasion-and-migration-in-human-gastric-cancer-through-upregulation-of-thrombospondin-1
#10
Tingting Huang, Lei Wang, Dian Liu, Piao Li, Huihua Xiong, Liang Zhuang, Li Sun, Xianglin Yuan, Hong Qiu
Fibroblast growth factor 7 (FGF7) is a mesenchyme-specific heparin-binding growth factor that binds FGF receptor 2 (FGFR2) to regulate numerous cellular and physiological processes. FGF7/FGFR2 signal is associated with gastric cancer progression. In the present study, we investigated the molecular mechanism by which FGF7/FGFR2 promotes invasion and migration in human gastric cancer. We first demonstrated that increased FGFR2 expression in human gastric cancer tissues was significantly associated with tumor depth and clinical stage in human gastric cancer tissues...
March 22, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28338788/hypertension-and-atrial-fibrillation-an-intimate-association-of-epidemiology-pathophysiology-and-outcomes
#11
Mikhail S Dzeshka, Farhan Shahid, Alena Shantsila, Gregory Y H Lip
Atrial fibrillation (AF) is the most prevalent sustained arrhythmia found in clinical practice. AF rarely exists as a single entity but rather as part of a diverse clinical spectrum of cardiovascular diseases, related to structural and electrical remodeling within the left atrium, leading to AF onset, perpetuation, and progression. Due to the high overall prevalence within the AF population arterial hypertension plays a significant role in the pathogenesis of AF and its complications. Fibroblast proliferation, apoptosis of cardiomyocytes, gap junction remodeling, accumulation of collagen both in atrial and ventricular myocardium all accompany ageing-related structural remodeling with impact on electrical activity...
February 18, 2017: American Journal of Hypertension
https://www.readbyqxmd.com/read/28338638/the-value-of-coenzyme-q10-determination-in-mitochondrial-patients
#12
REVIEW
Delia Yubero, George Allen, Rafael Artuch, Raquel Montero
Coenzyme Q10 (CoQ) is a lipid that is ubiquitously synthesized in tissues and has a key role in mitochondrial oxidative phosphorylation. Its biochemical determination provides insight into the CoQ status of tissues and may detect CoQ deficiency that can result from either an inherited primary deficiency of CoQ metabolism or may be secondary to different genetic and environmental conditions. Rapid identification of CoQ deficiency can also allow potentially beneficial treatment to be initiated as early as possible...
March 24, 2017: Journal of Clinical Medicine
https://www.readbyqxmd.com/read/28338575/the-role-of-cx43-mediated-nf%C3%B0%C2%BAb-signaling-pathway-in-ossification-of-posterior-longitudinal-ligament-an-in-vivo-and-in-vitro-study
#13
Dechun Chen, Yu Chen, Tiefeng Li, Lei Shi, Mengxiao Pan, Deyu Chen
STUDY DESIGN: In vivo and in vitro experiments. OBJECTIVES: To illustrate the further molecular mechanism of Cx43-mediated osteoblastic differentiation of ligament cells. SUMMARY OF BACKGROUND DATA: Ossification of the posterior longitudinal ligament (OPLL) is one of the main causes of myelopathy in Asian, but its etiology has not been clarified. We have previously found the mechanical stress can up-regulate Cx43 expression in ligament cells, which transduces mechanical signal to promote osteoblastic differentiation...
March 23, 2017: Spine
https://www.readbyqxmd.com/read/28338391/modifications-in-human-oral-fibroblast-ultrastructure-collagen-production-and-lysosomal-compartment-in-response-to-e-cigarette-fluids
#14
Silvia Sancilio, Marialucia Gallorini, Amelia Cataldi, Laura Sancillo, Rosa Alba Rana, Viviana di Giacomo
Background Many smokers have recently turned to electronic cigarettes (e-cigarettes) because they have been marketed as a cheaper and safer smokeless alternative to traditional cigarettes, and a possible smoking cessation tool. Even though the safety of these electronic devices is still not fully known, there is evidence of their cytotoxicity on cells which belong to the oral cavity. In a previous paper the increase of reactive oxygen production and Bax expression, followed by the occurrence of apoptosis, were demonstrated in human gingival fibroblasts...
March 24, 2017: Journal of Periodontology
https://www.readbyqxmd.com/read/28338113/antiproliferative-and-apoptotic-effects-of-lidocaine-on-human-hepatocarcinoma-cells-a-preliminary-study
#15
Ancuta Jurj, Ciprian Tomuleasa, Tiberiu T Tat, Ioana Berindan-Neagoe, Stefan V Vesa, Daniela C Ionescu
BACKGROUND AND AIMS: It is now well documented that certain anesthetic techniques may influence long term outcome in cancer patients undergoing surgery. More recently, local anesthetics proved certain antiproliferative effects in cancer cells. In our study, we aimed to investigate if lidocaine has antiproliferative effects in human hepatocarcinoma cells and to identify possible mechanisms of these effects. METHODS: We investigated the inhibitory effect of different concentrations of lidocaine on the proliferation of cultured HepG2 human hepatocarcinoma cells and LX2 normal liver fibroblasts...
March 2017: Journal of Gastrointestinal and Liver Diseases: JGLD
https://www.readbyqxmd.com/read/28337957/transplantation-of-cultured-olfactory-bulb-cells-prevents-abnormal-sensory-responses-during-recovery-from-dorsal-root-avulsion-in-the-rat
#16
Andrew Collins, Daqing Li, Stephen B McMahon, Geoffrey Raisman, Ying Li
The central branches of the C7 and C8 dorsal roots were avulsed close to their entry point into the spinal cord in adult rats. The forepaw responses to heat and cold stimuli were tested at 1, 2 and 3 weeks after injury. Over this period the paws were sensitive to both stimuli at 1-2 weeks and returned towards normal at 3 weeks. Immunohistology showed no evidence of axonal regeneration into the spinal cord in a control group of rats with avulsion only, implying that adjacent dorsal roots and their corresponding dermatomes were involved in the recovery...
March 23, 2017: Cell Transplantation
https://www.readbyqxmd.com/read/28337873/cyclosporine%C3%A2-a-augments-endoplasmic-reticulum-stress-markers-and-expression-of-matrix-protein-mrna
#17
S R Rao, R Subbarayan, D M Girija
Cyclosporine-A induces gingival overgrowth with disturbance in the homeostasis of cells and connective tissue proteins. Human gingival fibroblasts were cultured with cyclosporine A, and the expression of two vital endoplasmic stress markers and two prime matrix proteins (connective tissue growth factor (CTGF and periostin) were assessed by RT-PCR. We found that expression of Glucose-Regulated Protein 78 (GRP78/BIP) and CCAAT/enhancer binding protein, C/EBP homologous protein (CHOP) were significantly increased, along with CTGF and periostin, suggesting a role for these factors in gingival overgrowth...
January 2017: Journal of Biological Regulators and Homeostatic Agents
https://www.readbyqxmd.com/read/28337871/cytokine-modulation-in-patients-with-idiopathic-pulmonary-fibrosis-undergoing-treatment-with-steroids-immunosuppressants-and-ifn-%C3%AE-1b
#18
S Marinari, V De Iuliis, V Dadorante, S Colella, A Marino, A Nunziata, V Flati, M Caruso, A Pennelli, F De Benedetto, S Matera, S Capodifoglio, S Martinotti, S Caputi, E Toniato
Idiopathic pulmonary fibrosis (IPF) is a chronic lung disease of unknown etiology and pathogenic mechanisms. From an etiopathogenic point of view, alveolar macrophages play a key role in accumulation of fibroblasts and deposition of collagen and extracellular matrix by releasing specific cytokines and inflammatory mediators. IPF seems to be also associated with circulating fibrocytes, which might be involved with an abnormal pulmonary vascular repair and remodeling. Based on its hypothesized pathologic mechanisms, anti-inflammatory, anti-fibrotic and immunosuppressive therapies are often used...
January 2017: Journal of Biological Regulators and Homeostatic Agents
https://www.readbyqxmd.com/read/28337713/the-potential-role-of-fibroblast-growth-factor-21-in-lipid-metabolism-and-hypertension
#19
REVIEW
Zhe Huang, Aimin Xu, Bernard M Y Cheung
Fibroblast growth factor (FGF) 21 belongs to the FGF superfamily that is involved in cell proliferation and differentiation, neural development, angiogenesis, and metabolism. FGF21 requires β-Klotho as a co-receptor. Tissues involved in metabolism such as the liver, adipose tissues, skeletal muscle, and pancreas express FGF21. Starvation increases hepatic expression of FGF21, which then acts centrally to increase hepatic gluconeogenesis. FGF21 also increases fatty acid oxidation. This may be relevant in cold exposure, when expression of FGF21 is induced...
April 2017: Current Hypertension Reports
https://www.readbyqxmd.com/read/28337504/biotransformation-of-2-4-toluenediamine-in-human-skin-and-reconstructed-tissues
#20
Lisa Grohmann, Daniel Becker, Jörg Rademann, Nan Ma, Monika Schäfer-Korting, Günther Weindl
Reconstructed human epidermis (RHE) is used for risk assessment of chemicals and cosmetics and RHE as well as reconstructed human full-thickness skin (RHS) become important for e.g., the pre-clinical development of drugs. Yet, the knowledge regarding their biotransformation capacity is still limited, although the metabolic activity is highly relevant for skin sensitization, genotoxicity, and the efficacy of topical dermatics. The biotransformation of the aromatic amine 2,4-toluenediamine (2,4-TDA) has been compared in two commercially available RHS to normal human skin ex vivo, and in primary epidermal keratinocytes and dermal fibroblasts as well as in vitro generated epidermal Langerhans cells and dermal dendritic cells...
March 23, 2017: Archives of Toxicology
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