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https://www.readbyqxmd.com/read/29436170/simultaneous-determination-and-pharmacokinetics-study-of-four-quinones-in-rat-plasma-by-uhplc-esi-ms-ms-after-the-oral-administration-of-qianzhi-capsules
#1
Mingjie Gao, Zhibin Wang, Jing Yang, Liqian Wang, Chengcui Wu, Binbin Cui, Chunjuan Yang
A sensitive, specific, and accurate ultra high performance liquid chromatography with electrospray ionization tandem mass spectrometry method was developed and validated for the simultaneous quantification of purpurin, munjistin, mollugin and alizarin from Qianzhi capsules in rat plasma. Chromatographic separation was performed on an Aglient Eclipse Plus C18 RRHD column with a mobile phase consisting of methanol and 5 mM ammonium acetate/water with gradient elution. The analytes were quantified on a triple quadrupole mass spectrometer, operating in the multiple reaction monitoring mode and switching the electrospray ion source polarity with positive electrospray ionization in a single run...
February 13, 2018: Journal of Separation Science
https://www.readbyqxmd.com/read/29432387/quantification-of-phosphatidylethanols-in-whole-blood-as-a-proxy-for-chronic-alcohol-consumption-using-ultra-performance-convergence-chromatography-tandem-mass-spectrometry
#2
Bart C H van der Nagel, Sophie Wassenaar, Soma Bahmany, Birgit C P Koch
BACKGROUND: Detection of alcohol consumption after a longer period of time can be useful in certain patient groups. To monitor chronic alcohol consumption, a novel analytical method for the quantification of phosphatidylethanols (PEth) was developed and validated using ultra performance convergence chromatography tandem mass spectrometry. METHODS: The main phosphatidylethanols -Palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanol (POPEth), 1-Palmitoyl-2-linoleoyl-sn-glycero-3-phosphoethanol (PLPEth), and 1,2-Dioleoyl-sn-glycero-3-phosphoethanol (DOPEth) were analyzed using a simple and fast sample preparation protocol followed by chromatographic separation using ultra performance convergence chromatography, a novel kind of supercritical fluid chromatography...
February 8, 2018: Therapeutic Drug Monitoring
https://www.readbyqxmd.com/read/29424335/simple-and-rapid-uplc-ms-ms-method-for-quantification-of-entecavir-in-human-plasma-application-to-a-bioequivalence-study%C3%A2
#3
Zhen-Yan Hou, Miao Yan, Hua-Lin Cai, Yang Deng, Hui-Hui Wu, Zhi Tang, Xiao-Yang Hu, Xi-Ding Yang, Bi-Kui Zhang
A simple and fast ultra-performance liquid chromatography-tandem mass spectrometry method was developed and validated to determine entecavir in human plasma with the stable isotopically labeled internal standard entecavir-13C215N. Samples (100 µL each) were pretreated by protein precipitation with methanol, and then separated on an ACQUITY UPLC BEH C18 analytical column (2.1 × 50 mm, 1.7 µm) with a simple isocratic elution. The detection was operated by a positive ionization electrospray mass spectrometry in multiple reaction monitoring mode...
February 9, 2018: International Journal of Clinical Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/29420107/development-of-a-rapid-fluorescence-based-high-throughput-screening-assay-to-identify-novel-kynurenine-3-monooxygenase-inhibitor-scaffolds
#4
K R Jacobs, G J Guillemin, D B Lovejoy
Kynurenine 3-monooxygenase (KMO) is a well-validated therapeutic target for the treatment of neurodegenerative diseases, including Alzheimer's disease (AD) and Huntington's disease (HD). This work reports a facile fluorescence-based KMO assay optimized for high-throughput screening (HTS) that achieves a throughput approximately 20-fold higher than the fastest KMO assay currently reported. The screen was run with excellent performance (average Z' value of 0.80) from 110,000 compounds across 341 plates and exceeded all statistical parameters used to describe a robust HTS assay...
February 1, 2018: SLAS Discovery
https://www.readbyqxmd.com/read/29413579/enantiomeric-separation-and-quantification-of-r-s-amphetamine-in-urine-by-ultra-high-performance-supercritical-fluid-chromatography-tandem-mass-spectrometry
#5
S Hegstad, H Havnen, A Helland, O Spigset, J Frost
To distinguish between legal and illegal consumption of amphetamine reliable analytical methods for chiral separation of the R- and S-enantiomers of amphetamine in biological specimens are required. In this regard, supercritical fluid chromatography (SFC) has several potential advantages over liquid chromatography, including rapid separation of enantiomers due to low viscosity and high diffusivity of supercritical carbon dioxide, the main component in the SFC mobile phase. A method for enantiomeric separation and quantification of R- and S-amphetamine in urine was developed and validated using ultra-high performance supercritical fluid chromatography-tandem mass spectrometry (UHPSFC-MS/MS)...
January 29, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29404062/a-validated-uplc-ms-ms-method-for-simultaneous-determination-of-imatinib-dasatinib-and-nilotinib-in-human-plasma
#6
Jing Zeng, Hua Lin Cai, Zhi Ping Jiang, Qing Wang, Yan Zhu, Ping Xu, Xie Lan Zhao
A sensitive, rapid, simple and economical ultra-performance liquid chromatography-tandem mass spectrometric method (UPLC-MS/MS) was developed and validated for simultaneous determination of imatinib, dasatinib and nilotinib in human plasma using gliquidone as internal standard (IS). Liquid-liquid extraction method with ethyl acetate was used for sample pre-treatment. The separation was performed on an Xtimate Phenyl column using isocratic mobile phase consisting of A (aqueous phase: 0.15% formic acid and 0...
December 2017: Journal of Pharmaceutical Analysis
https://www.readbyqxmd.com/read/29402417/a-novel-method-for-quantitation-of-acylglycines-in-human-dried-blood-spots-by-uplc-tandem-mass-spectrometry
#7
Lawrence Fisher, Christine Davies, Osama Y Al-Dirbashi, Herman J Ten Brink, Pranesh Chakraborty, Nathalie Lepage
BACKGROUND: Several acylcarnitines used as primary markers on dried blood filter papers (DBS) for newborn screening lack specificity and contribute to a higher false positive rate. The analysis of urine acylglycines is useful in the diagnosis of inborn errors of metabolism (IEM) including medium chain acyl-CoA dehydrogenase deficiency (MCADD), isovaleric acidemia, and beta-ketothiolase deficiency (BKTD). Currently, no method for analyzing acylglycines from DBS has been published. METHODS: Acylglycines were extracted from two 3...
February 2, 2018: Clinical Biochemistry
https://www.readbyqxmd.com/read/29378427/ultra-obligatory-running-among-ultramarathon-runners
#8
Martin D Hoffman, Rhonna Krouse
Participants in the Ultrarunners Longitudinal TRAcking (ULTRA) Study were asked to answer "yes" or "no" to the question "If you were to learn, with absolute certainty, that ultramarathon running is bad for your health, would you stop your ultramarathon training and participation?" Among the 1349 runners, 74.1% answered "no". Compared with those answering "yes", they were younger (p < 0.0001), less likely to be married (p = 0.019), had less children (p = 0.0095), had a lower health orientation (p < 0...
January 29, 2018: Research in Sports Medicine
https://www.readbyqxmd.com/read/29373719/quantification-of-15-psychotropic-drugs-in-serum-and-postmortem-blood-samples-after-a-modified-mini-quechers-by-uhplc-ms-ms
#9
A Pouliopoulos, E Tsakelidou, A Krokos, H G Gika, G Theodoridis, N Raikos
The aim of the study was to develop a LC-MS-MS method able to detect and quantify a number of frequently prescribed antipsychotic and antidepressant drugs for toxicological purposes. Separation of compounds was performed on a C-18 RP column by Ultra High-Pressure Chromatography over a 11 min run. A modified single step QuEChERS protocol consisted essentially by the addition of acetonitrile, potassium carbonate and magnesium sulfate in 100 μL of sample, vortexing, centrifugation and evaporation has been selected...
January 24, 2018: Journal of Analytical Toxicology
https://www.readbyqxmd.com/read/29372167/a-biomechanical-assessment-of-biceps-femoris-repair-techniques
#10
Eric A Branch, Dustin Loveland, Sohale Sadeghpour, Adam W Anz
Background: Knee injuries encountered in clinical practice can involve avulsions of the biceps femoris from the fibula and proximal tibia. Advances in tendon repair methods now allow for repairs with increased surface areas using modern suture anchor techniques. Despite descriptions of repair techniques, there are no biomechanical studies on the biceps femoris for comparison. Purpose/Hypothesis: The objective of this controlled laboratory study was to determine the failure load of the native biceps femoris distal insertion and to evaluate modern repair techniques...
January 2018: Orthopaedic Journal of Sports Medicine
https://www.readbyqxmd.com/read/29356949/validity-of-the-polar-v800-monitor-for-measuring-heart-rate-variability-in-mountain-running-route-conditions
#11
Pere Caminal, Fuensanta Sola, Pedro Gomis, Eduard Guasch, Alexandre Perera, Núria Soriano, Lluis Mont
PURPOSE: This study was conducted to test, in mountain running route conditions, the accuracy of the Polar V800™ monitor as a suitable device for monitoring the heart rate variability (HRV) of runners. METHOD: Eighteen healthy subjects ran a route that included a range of running slopes such as those encountered in trail and ultra-trail races. The comparative study of a V800 and a Holter SEER 12 ECG Recorder™ included the analysis of RR time series and short-term HRV analysis...
January 22, 2018: European Journal of Applied Physiology
https://www.readbyqxmd.com/read/29340161/direct-ice-imaging-from-inside-the-left-atrial-appendage-during-ablation-of-persistent-atrial-fibrillation
#12
Gabriel Cismaru, Mihai Puiu, Radu Rosu, Lucian Muresan, Gabriel Gusetu, Dana Pop, Dumitru Zdrenghea
We present the case of a 59-year-old patient with persistent atrial fibrillation, referred for atrial fibrillation ablation. The procedure was performed with the help of NAVX 3D mapping system (Saint Jude Medical) and iLAB Ultra ICE Plus ultrasound imaging catheter (Boston Scientific). The catheter permits cross-sectional images perpendicular to catheter's long axis. From inside left atrial appendage (LAA) looks trabeculated, due to pectinate muscles running parallel to each other. The presence of a thrombus was excluded from the appendage...
January 2018: Oxford Medical Case Reports
https://www.readbyqxmd.com/read/29325818/simultaneous-determination-of-voriconazole-posaconazole-itraconazole-and-hydroxy-itraconazole-in-human-plasma-using-lcms-ms
#13
Nicole Jenkins, Marion Black, Hans G Schneider
INTRODUCTION: Invasive fungal infections are an increasing cause of mortality and morbidity in high risk patient populations such as those on immunosuppressive therapy. Triazole antifungals are recommended for the prevention and treatment of such infections. The aim of this study was to develop and validate a simple, sensitive and robust LCMS/MS method for the simultaneous analysis in human plasma of three frequently used antifungal drugs: voriconazole, posaconazole, and itraconazole...
January 9, 2018: Clinical Biochemistry
https://www.readbyqxmd.com/read/29276983/uplc-ms-ms-method-for-the-simultaneous-quantification-of-sofosbuvir-sofosbuvir-metabolite-gs-331007-and-daclatasvir-in-plasma-of-hiv-hcv-co-infected-patients
#14
Stefania Notari, Massimo Tempestilli, Gabriele Fabbri, Raffaella Libertone, Andrea Antinori, Adriana Ammassari, Chiara Agrati
Direct-acting antiviral agents (DAAs) represent the major advance in hepatitis C virus (HCV) infection treatment leading to extremely high eradication rates in HCV mono- and HIV/HCV co-infected patients. In this scenery, availability of Therapeutic Drug Monitoring (TDM) is of interest to assess plasma concentrations to prevent either therapeutic failure due to suboptimal medication adherence and drug-drug interactions or avoid adverse events. Aim of this study was to develop and validate an Ultra-Performance Liquid Chromatography Mass Spectrometry (UPLC-MS/MS) method for the simultaneous quantification of sofosbuvir, sofosbuvir metabolite (GS-331007), and daclatasvir in human plasma...
December 12, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29276835/a-sensitive-high-throughput-and-robust-trapping-micro-lc-ms-strategy-for-quantification-of-biomarkers-and-antibody-biotherapeutics
#15
Ming Zhang, Bo An, Yang Qu, Shichen Shen, Wei Fu, Yuan-Ju Chen, Xue Wang, Rebeccah Young, John M Canty, Joseph P Balthasar, Keeley Murphy, Debadeep Bhattacharyya, Jonathan L Josephs, Luca Ferrari, Shaolian Zhou, Surendra Bansal, Faye Vazvaei, Jun Qu
For LC-MS-based targeted quantification of biotherapeutics/biomarkers in clinical and pharmaceutical environments, high sensitivity, high throughput, and excellent robustness are all essential, but remain challenging. For example, though nano-LC-MS has been employed to enhance analytical sensitivity, it falls short due to its low loading capacity, poor throughput and low operational robustness. Furthermore, high chemical noise in protein bioanalysis typically limits the sensitivity. Here we describe a novel Trapping-micro-LC-MS (T-µLC-MS) strategy for targeted protein bioanalysis, achieving high sensitivity with exceptional robustness and high throughput...
December 25, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29259560/the-energetics-during-the-world-s-most-challenging-mountain-ultra-marathon-a-case-study-at-the-tor-des-geants%C3%A2
#16
Aldo Savoldelli, Alessandro Fornasiero, Pietro Trabucchi, Eloisa Limonta, Antonio La Torre, Francis Degache, Barbara Pellegrini, Grégoire P Millet, Gianluca Vernillo, Federico Schena
Purpose: To provide insights into the energy requirements as well as the physiological adaptations of an experienced 50-year-old ultra-marathon male athlete during the world's most challenging mountain ultra-marathon (MUM). Methods: The international race supporting the study was the Tor des Geants®, characterized by 330 km with +24,000 m D+ to be covered within 150 h. Before the MUM, we assessed the peak oxygen uptake ([Formula: see text]) by means of an incremental graded running test. During the MUM we monitored six ascents (once per race day) with a portable gas analyzer, a GPS and a finger pulse oximeter...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/29241333/online-hplc-esi-hrms-method-for-the-analysis-and-comparison-of-different-dissolved-organic-matter-samples
#17
Claudia Patriarca, Jonas Bergquist, Per J R Sjöberg, Lars Tranvik, Jeffrey Alistair Hawkes
Natural dissolved organic matter (DOM) is an ultra-complex mixture that is essential to global carbon cycling, but is poorly understood because of its complexity. The most powerful tool for the DOM characterization is high-resolution mass spectrometry (HRMS) generally combined to direct infusion (DI) as sample introduction. Liquid chromatography (LC) represents a compelling alternative to DI; however state-of-the-art techniques involve only offline LC-HRMS approaches, which have important logistical drawbacks that make DOM analysis more challenging...
December 15, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/29238569/transient-cardiac-dysfunction-but-elevated-cardiac-and-kidney-biomarkers-24%C3%A2-h-following-an-ultra-distance-running-event-in-mexican-tarahumara
#18
Dirk L Christensen, Diana Espino, Rocío Infante-Ramírez, Mónica S Cervantes-Borunda, Rosa P Hernández-Torres, Antonio E Rivera-Cisneros, Daniel Castillo, Kate Westgate, Dijana Terzic, Soren Brage, Christian Hassager, Jens P Goetze, Jesper Kjaergaard
Background: The Mexican Tarahumara are accustomed to running ultra-distance races. No data exist on the acute physiological changes following ultra-distance running and physiological-biomarker associations in this population. Thus, we aimed to investigate the acute impact (≤ 24 h) on functional and biochemical changes of the cardiac muscle and biochemical changes associated with kidney function following a 63-km ultra-distance race with an altitude difference of 1800 m in Mexican Tarahumara athletes...
2017: Extreme Physiology & Medicine
https://www.readbyqxmd.com/read/29236950/discrimination-and-chemical-phylogenetic-study-of-four-pulsatilla-herbs-using-uplc-esi-ms-ms-combined-with-hierarchical-cluster-analysis
#19
Miao-Miao Jin, Wen-Dan Zhang, Geng-Shen Song, Yan-Mei Xu, Ying-Feng Du, Wei Guo, Liang Cao, Hui-Jun Xu
A tissue-smashing based ultra-rapid extraction coupled with ultra-performance liquid chromatography tandem-mass spectrometry (UPLC-MS/MS) method was developed to determine 10 major triterpenoid saponins from Pulsatilla herbs. Compound 4 was characterized as betulinic acid glycoside 3-O-α-arabinopyranosyl-28-O-β-glucopyranosyl-23-hydroxy with HR-ESI-MS, 1H-NMR and 13C-NMR experiment. The MS spectra result showed that the ionization of compound 4 was more efficient in the positive mode. Meanwhile, the ions at m/z 789...
December 11, 2017: Journal of Chromatographic Science
https://www.readbyqxmd.com/read/29192842/validity-of-an-ultra-wideband-local-positioning-system-to-measure-locomotion-in-indoor-sports
#20
F R Serpiello, W G Hopkins, S Barnes, J Tavrou, G M Duthie, R J Aughey, K Ball
The validity of an Ultra-wideband (UWB) positioning system was investigated during linear and change-of-direction (COD) running drills. Six recreationally-active men performed ten repetitions of four activities (walking, jogging, maximal acceleration, and 45º COD) on an indoor court. Activities were repeated twice, in the centre of the court and on the side. Participants wore a receiver tag (Clearsky T6, Catapult Sports) and two reflective markers placed on the tag to allow for comparisons with the criterion system (Vicon)...
December 1, 2017: Journal of Sports Sciences
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