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Lu-Bin Zhong, Qing Liu, Peng Wu, Qi-Feng Niu, Huan Zhang, Yu-Ming Zheng
Aquatic ecosystem and human health have been seriously threatened by illegal discharge of wastewater, while simple and effective monitoring methods are still sparse. Here, we propose a facile method for on-site pollutant monitoring by surface enhanced Raman spectroscopy (SERS) with a novel substrate. This substrate is fabricated by interface self-assembly of Au@Ag nanocubes (NCs) on a simultaneously formed polyvinyl chloride (PVC) template, followed by coating a thin Au film. The Au@Ag@Au-NCs/PVC film is flexible, ultra-light and robust, which could float on water surface and firmly contact with water even under harsh environmental conditions...
April 16, 2018: Environmental Science & Technology
Shan Lin, Xinyi Yue, Danwei Ouyang, Quan Li, Peiming Yang
Run-zao-zhi-yang (RZZY) capsule, a traditional Chinese medicine formula (TCMF), has been popularly used for the treatment of dermatitis and eczema. However, few studies have been carried out on RZZY and its metabolites. In this study, we developed a three-step strategy to rapidly characterize the chemical constituents and metabolites of RZZY by using ultra high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. A total of 41 chemical components were characterized from RZZY...
April 12, 2018: Biomedical Chromatography: BMC
Mikhail Ignatov, Andrey Kazennov, Dima Kozakov
We have recently demonstrated that incorporation of SAXS-based filtering in our heavily used docking server ClusPro improves docking results. However, the filtering step is time consuming, since ≈105 conformations have to be sequentially processed. At the same time we have demonstrated the possibility of ultra-fast systematic energy evaluation for all rigid body orientations of two proteins, by sampling using Fast Manifold Fourier Transform (FMFT), if energies are represented as a combination of convolution-like expressions...
April 4, 2018: Journal of Molecular Biology
Juan Li, Xueling Ren, Yanyan Diao, Yu Chen, Qingli Wang, Wenting Jin, Piao Zhou, Qiqi Fan, Yanbing Zhang, Hongmin Liu
The aim of this work was to develop a simple and rapid analytical method for the simultaneous determination of a wide range of drug residues in milk by UHPLC-MS/MS. A total of 25 typical veterinary drugs investigated belong to six families including β-lactams, quinolones, β-agonists, phenicols, glucocorticoids and nitrofurans. The samples were extracted by acetonitrile and defatted with n-hexane twice. Electrospray ionization and positive/negative polarity switching were utilized for the analysis of 25 veterinary drugs in a single chromatographic run...
August 15, 2018: Food Chemistry
Michael J Palte, Sankha S Basu, Jameson L Dahlin, Ralitsa Gencheva, Donald Mason, Petr Jarolim, Athena K Petrides
BACKGROUND: Therapeutic drug monitoring (TDM) of antiepileptic drugs (AEDs) is often necessary to prevent associated destructive toxicities. Tandem mass spectrometry (MS/MS) with stable-isotope-labeled (SIL) internal standards (ISs) is considered the gold standard for the measurement of AEDs. This study presents the development and validation of a clinical ultra-performance liquid chromatography (U-HPLC)-MS/MS method for the concurrent measurement of gabapentin, lamotrigine, levetiracetam, monohydroxy derivative (MHD) of oxcarbazepine, and zonisamide in human serum...
April 3, 2018: Therapeutic Drug Monitoring
Kan Luo, Zhipeng Cai, Keqin Du, Fumin Zou, Xiangyu Zhang, Jianqing Li
Energy efficiency is still the obstacle for long-term real-time wireless ECG monitoring. In this paper, a digital compressed sensing- (CS-) based single-spot Bluetooth ECG node is proposed to deal with the challenge in wireless ECG application. A periodic sleep/wake-up scheme and a CS-based compression algorithm are implemented in a node, which consists of ultra-low-power analog front-end, microcontroller, Bluetooth 4.0 communication module, and so forth. The efficiency improvement and the node's specifics are evidenced by the experiments using the ECG signals sampled by the proposed node under daily activities of lay, sit, stand, walk, and run...
2018: Journal of Healthcare Engineering
Viviane G Nasr, Jonathan Meserve, Luis M Pereira, David Faraoni, Steve Brediger, Susan Goobie, Ravi Thiagarajan, James A DiNardo
Patient sedation and analgesia on extracorporeal membrane oxygenation (ECMO) is vital for safety and comfort. However, adsorption to the circuit may alter drug pharmacokinetics and remains poorly characterized. This study characterizes drug adsorption of morphine, fentanyl, midazolam, and dexmedetomidine in an ex vivo infant ECMO circuit utilizing polymethylpentene (PMP) membrane oxygenator (MO) with protein-bounded polyvinylchloride (PVC) tubing. Twelve closed-loop ex vivo ECMO circuits were prepared using P...
March 26, 2018: ASAIO Journal: a Peer-reviewed Journal of the American Society for Artificial Internal Organs
Libor Trško, František Nový, Otakar Bokůvka, Michal Jambor
Ultrasonic fatigue testing is one of a few methods which allow investigating fatigue properties in the ultra-high cycle region. The method is based on exposing the specimen to longitudinal vibrations on its resonance frequency close to 20 kHz. With use of this method, it is possible to significantly decrease the time required for the test, when compared to conventional testing devices usually working at frequencies under 200 Hz. It is also used to simulate loading of material during operation in high speed conditions, such as those experienced by components of jet engines or car turbo pumps...
March 7, 2018: Journal of Visualized Experiments: JoVE
Ying Zhu, Rui Zhao, Paul D Piehowski, Ronald J Moore, Sujung Lim, Victoria J Orphan, Ljiljana Paša-Tolić, Wei-Jun Qian, Richard D Smith, Ryan T Kelly
One of the greatest challenges for mass spectrometry (MS)-based proteomics is the limited ability to analyze small samples. Here we investigate the relative contributions of liquid chromatography (LC), MS instrumentation and data analysis methods with the aim of improving proteome coverage for sample sizes ranging from 0.5 ng to 50 ng. We show that the LC separations utilizing 30-μm- i.d. columns increase signal intensity by >3-fold relative to those using 75-μm- i.d. columns, leading to 32% increase in peptide identifications...
April 2018: International Journal of Mass Spectrometry
Jin-Jun Hou, Chun-Mei Cao, Yong-Wei Xu, Shuai Yao, Lu-Ying Cai, Hua-Li Long, Qi-Rui Bi, Yuan-Yuan Zhen, Wan-Ying Wu, De-An Guo
BACKGROUND: Lipids, a group of primary metabolites, could be used as quality markers of Traditional Chinese medicine. PURPOSE: The present study was designed to develop a research method to explore lipid markers of the quality of coix seeds with different geographical origins. STUDY DESIGN: The geographical origins of coix seeds were divided into three regions based on the latitude. A central composite design (CCD test) was used to optimize the chromatographic parameters of supercritical fluid chromatography to obtain optimal lipid profile of coix seed...
March 7, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
Stefan Zielen, Jennis Gabrielpillai, Eva Herrmann, Johannes Schulze, Ralf Schubert, Martin Rosewich
BACKGROUND: Ultra-short course pollen immunotherapy adjuvanted with monophosphoryl lipid A (MPL) is attractive to conventional allergen-specific immunotherapy (AIT). Long term efficacy of MPL-AIT has not been evaluated. METHODS: 68 patients (age 16.75 ± 5.3 years) with allergic rhinitis to grass pollen were investigated. Group 1: 21 controls; Group 2: 19 after complete AIT, and Group 3: 28 with AIT and treatment cessation: 4 years range 3-6 years ago. RESULTS: The clinical symptoms (running nose, sneezing, conjunctivitis and the weekly overall score) were significantly reduced in patients group 2 and 3 compared with controls without AIT p < 0...
March 22, 2018: Immunotherapy
Zhaohui Qiu, Jie Peng, Lingli Mou, Xiao Li, Fanqi Meng, Peng Yu
Multidrug resistance (MDR) of tumors occurs when tumor cells exhibit reduced sensitivity to a large number of unrelated drugs. The molecular mechanism of MDR commonly involves overexpression of the plasma membrane drug efflux pump P-glycoprotein (P-gp). Overexpression of P-gp may be induced by the selection and/or adaptation of cells during exposure to chemotherapeutic drugs, referred to as acquired P-gp-mediated MDR. This study aimed to establish a P-gp quantification method by Ultra Performance Liquid Chromatography and Tandem Mass Spectrometry (UPLC-MS/MS) to better understand the regulation of P-gp expression and its relationship with the level of drug resistance...
March 8, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Shih-Liang Hsi, Peter Surman, Raida Al-Kassas
A highly sensitive and rapid stability indicating Ultra Performance Liquid Chromatographic (UPLC) method was developed for the quantification and identification of isotretinoin in bulk. Chromatographic separation was developed using a gradient elution in a reversed-phase system at flow rate of 0.5 ml/min with 12 minutes run time. The mobile phase was a gradient mixture of mobile phase A (contained a 30:70:0.5 mixture solution of methanol/Purified water/Glacial Acetic Acid) and mobile phase B (contained a 70:25:4...
March 20, 2018: Pharmaceutical Development and Technology
Wei Sun, Stacy Ho, Xiaojun Rick Fang, Thomas O'Shea, Hanlan Liu
An ultra-high pressure liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was successfully developed and qualified for the simultaneous determination of triamcinolone hexacetonide (TAH) and triamcinolone acetonide (TAA, the active metabolite of TAH) in rabbit plasma. To prevent the hydrolysis of TAH to TAA ex vivo during sample collection and processing, we evaluated the effectiveness of several esterase inhibitors to stabilize TAH in plasma. Phenylmethanesulfonyl fluoride (PMSF) at 2.0 mM was chosen to stabilize TAH in rabbit plasma...
May 10, 2018: Journal of Pharmaceutical and Biomedical Analysis
Andrey Fokin, Mikhail Glyavin, German Golubiatnikov, Lev Lubyako, Mikhail Morozkin, Boris Movschevich, Alexander Tsvetkov, Gregory Denisov
Many state-of-the-art fundamental and industrial projects need the use of terahertz radiation with high power and small linewidth. Gyrotrons as radiation sources provide the desired level of power in the sub-THz and THz frequency range, but have substantial free-running frequency fluctuations of the order of 10-4 . Here, we demonstrate that the precise frequency stability of a high-power sub-THz gyrotron can be achieved by a phase-lock loop in the anode voltage control. The relative width of the frequency spectrum and the frequency stability obtained for a 0...
March 12, 2018: Scientific Reports
Deepak Bhandari, Brett A Bowman, Anish B Patel, David M Chambers, Víctor R De Jesús, Benjamin C Blount
This work describes a quantitative high-throughput analytical method for the simultaneous measurement of small aliphatic nitrogenous biomarkers, i.e., 1,6-hexamethylenediamine (HDA), isophoronediamine (IPDA), β-methylamino-l-alanine (BMAA), and trimethylamine N-oxide (TMAO), in human urine. Urinary aliphatic diamines, HDA and IPDA, are potential biomarkers of environmental exposure to their corresponding diisocyanates. Urinary BMAA forms as a result of human exposure to blue-green algae contaminated food. And, TMAO is excreted in urine due to the consumption of carnitine- and choline-rich diets...
March 2, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Ruiguo Wang, Wei Zhang, Peilong Wang, Xiaoou Su
An ultrasensitive paper based lateral flow assay is described for rapid and simultaneous fluorometric detection of several β-agonists including clenbuterol and its chemical analogues (mabuterol, brombuterol, cimaterol, cimbuterol, bromchlorbuterol and banbuterol). A nonspecific monoclonal antibody (mAb) against clenbuterol and its analogues was prepared and employed in a competitive immunoassay where mAb conjugated to fluorescent nanoparticles and free β-agonists compete for the binding sites. This enables rapid screening for the 7 β-agonists in a single run that takes about 8 min...
2018: Mikrochimica Acta
Shenglan Qi, Huida Guan, Gang Deng, Tao Yang, Xuemei Cheng, Wei Liu, Ping Liu, Changhong Wang
Adenosine deaminase (ADA), which is a key enzyme in the metabolism of purine nucleosides, plays important roles in diverse disorders, such as tuberculosis, diabetes, liver disorders, and cancer. Determination of the activities of ADA and its isoenzymes in body fluids has received considerable attention in the diagnosis and treatment of relative diseases. Ultraviolet spectroscopy with adenosine (AD) as a substrate is a classical approach for screening potential ADA inhibitors by measuring the decrease in substrate (AD) at 265 nm or increase in the product (inosine) at 248 nm...
February 22, 2018: Journal of Pharmaceutical and Biomedical Analysis
Andrew Michaels, Eli Yablonovitch
Efficient coupling between integrated optical waveguides and optical fibers is essential to the success of silicon photonics. While many solutions exist, perfectly vertical grating couplers that scatter light out of a waveguide in the direction normal to the waveguide's top surface are an ideal candidate due to their potential to reduce packaging complexity. Designing such couplers with high efficiencies, however, has proven difficult. In this paper, we use inverse electromagnetic design techniques to optimize a high efficiency two-layer perfectly vertical silicon grating coupler...
February 19, 2018: Optics Express
Hai H Bui, Phillip E Sanders, Diane Bodenmiller, Ming Shang Kuo, Gregory P Donoho, Anthony S Fischl
Phosphatidylinositol (3,4,5) trisphosphate (PIP3 ) is a biologically active membrane phospholipid that is essential for the growth and survival of all eukaryotic cells. We describe a new method that directly measures PIP3 and describe the HPLC separation and measurement of the positional isomers of phosphatidylinositol bisphosphate, PI(3,5)P2 , PI(3,4)P2 and PI(4,5)P2 . Mass spectrometric analyses were performed online using ultra-high performance liquid chromatography (UHPLC)-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) in the negative multiple-reaction monitoring (MRM) modes...
February 19, 2018: Analytical Biochemistry
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