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https://www.readbyqxmd.com/read/28818804/an-on-spot-internal-standard-addition-approach-for-accurately-determining-colistin-a-and-colistin-b-in-dried-blood-spots-using-ultra-high-performance-liquid-chromatography-tandem-mass-spectrometry
#1
I-Lin Tsai, Ching-Hua Kuo, Hsin-Yun Sun, Yu-Chung Chuang, Divyabharathi Chepyala, Shu-Wen Lin, Yun-Jung Tsai
Outbreaks of multidrug-resistant Gram-negative bacterial infections have been reported worldwide. Colistin, an antibiotic with known nephrotoxicity and neurotoxicity, is now being used to treat multidrug-resistant Gram-negative strains. In this study, we applied an on-spot internal standard addition approach coupled with an ultra high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to quantify colistin A and B from dried blood spots (DBSs). Only 15μL of whole blood was required for each sample...
August 3, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28799161/performance-factors-in-a-mountain-ultramarathon
#2
Pascal Balducci, Michel Clémençon, Robin Trama, Yoann Blache, Christophe Hautier
This study aimed to determine mountain ultra-marathon (MUM) performance factors in a large group of endurance mountain runners. Maximal aerobic speed (MAS) was assessed one week prior to the MUM. The level and graded (10%) energy cost of running, stiffness, knee extensors force (KEf), and jump height on a counter movement jump (CMJ) were measured in 24 male ultra runners before (pre) and immediately after (post) the Interlacs Trail (75 km and 3 930/3 700 m d+/d-). Performance time was correlated with MAS (r=- 0...
August 10, 2017: International Journal of Sports Medicine
https://www.readbyqxmd.com/read/28790928/enhanced-right-chamber-remodeling-in-endurance-ultra-trail-athletes-compared-to-marathon-runners-detected-by-standard-and-speckle-tracking-echocardiography
#3
Kristian Ujka, Luca Bastiani, Gennaro D'Angelo, Bruna Catuzzo, Alessandro Tonacci, Simona Mrakic-Sposta, Alessandra Vezzoli, Guido Giardini, Lorenza Pratali
Background: Strenuous and endurance exercise training have been associated with morphological and functional heart remodeling. Two-dimensional speckle-tracking echocardiography (STE) is a novel technique that allows an accurate quantification of global myocardium deformation. Our aim was to evaluate together left and right cardiac remodeling in different long-distance running athletes: marathon runners (42 km) (M) and endurance mountain runners (>300 Km) (UT). Methods: A total of 92 athletes (70 males, 76%) including 47 M [age 45 ± 7 years; training: 18 (9-53) years(*)days/week], 45 UT [age 42 ± 9, training: 30 (15-66) years(*)days/week] underwent conventional echocardiography and STE (Beyond Diogenes 2...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28738654/development-of-a-sensitive-and-quantitative-uhplc-ms-ms-method-to-study-the-whole-body-uptake-of-pharmaceuticals-in-zebrafish
#4
Stanislav Kislyuk, Jerome Kroonen, Erwin Adams, Patrick Augustijns, Peter de Witte, Deirdre Cabooter
An analytical procedure to measure the whole-body uptake of pharmaceuticals in zebrafish has been developed using state-of-the-art methodologies. A sample preparation procedure for 9 pharmaceuticals displaying a variety in physicochemical properties was developed using 10-day old zebrafish (TG898). For an efficient homogenization of the samples and subsequent recovery of the compounds of interest, different amounts of organic solvents in combination with acidic modifiers were added to zebrafish samples. Samples were subsequently processed using a powerful bath sonicator and centrifuged...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28715684/enantiomeric-separation-and-quantification-of-citalopram-in-serum-by-ultra-high-performance-supercritical-fluid-chromatography-tandem-mass-spectrometry
#5
Solfrid Hegstad, Hilde Havnen, Arne Helland, Berit Margrethe Hasle Falch, Olav Spigset
A method for enantiomeric separation and quantification of R/S-citalopram in serum was developed and validated using ultra-high performance supercritical fluid chromatography-tandem mass spectrometry (UHPSFC-MS/MS). Sample preparation prior to UHPSFC-MS/MS analysis consisted of protein precipitation with acidic acetonitrile and filtration through a phospholipid removal plate. The UHPSFC-MS/MS method used an UPC(2) Trefoil CEL2 column with a mobile phase consisting of CO2 and methanol/acetonitrile (70:30, v/v) with 10mM ammonium acetate...
July 9, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28709014/a-rapid-liquid-chromatography-tandem-mass-spectrometry-for-the-quantification-of-fosfomycin-in-plasma-urine-and-aqueous-fluids
#6
Nahed El-Najjar, Jonathan Jantsch, André Gessner
A simple and fast ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for the analysis of Fosfomycin in different matrices (human plasma/urine, and aqueous fluid) has been established and validated. Sample cleanup consists, depending on the matrix used, on protein precipitation or dilution with methanol containing isotopically labeled Fosfomycin as internal standard. Compounds, separated on a Luna Omega PS C18 column, were detected in multiple reactions monitoring in negative ion mode using API4000 system...
June 27, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28696782/development-of-a-novel-rapid-multiplex-polymerase-chain-reaction-assay-for-the-detection-and-differentiation-of-salmonella-enterica-serovars-enteritidis-and-typhimurium-using-ultra-fast-convection-polymerase-chain-reaction
#7
Tae-Hoon Kim, Hyun Jin Hwang, Jeong Hee Kim
Salmonella enterica serovars Enteritidis and Typhimurium are the most common causative agents of human nontyphoidal salmonellosis. The rapid detection and timely treatment of salmonellosis are important to increase the curative ratio and prevent spreading of the disease. In this study, we developed a rapid multiplex convection polymerase chain reaction (PCR) method to detect Salmonella spp. and differentiate Salmonella Enteritidis and Salmonella Typhimurium. We used the invA gene for Salmonella spp. detection...
July 11, 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/28674598/cortisol-and-alpha-amylase-changes-during-an-ultra-running-event
#8
Whitney P Deneen, Alexis B Jones
Elevated stress hormone concentrations can positively affect an athlete's overall performance during a competition, and in many cases, are necessary to be able to perform exercise. During extreme exercise, the body's ability to utilize energy efficiently can affect an athlete's performance. Elevated hormonal concentrations can have many benefits in regards to an athlete's overall performance during a competition. The purpose of this study was to examine the effects of long distance running, such as seen during an ultra-running event (distances beyond 26...
2017: International Journal of Exercise Science
https://www.readbyqxmd.com/read/28667982/a-gyro-stabilized-platform-leveling-loop-for-marine-gravimeter
#9
P F Wu, L T Liu, L Wang, Y Wang, M Zhong, Z B Zhou, Z Zou
An ultra-low-frequency platform leveling loop based on a mixed sensitivity H(∞) approach, which considers both the system bandwidth and response speed, was designed and applied to a prototype, two-axis gyro-stabilized platform marine gravimeter CHZ-II. The instrument was developed for regional surveys in deep ocean areas where high-resolution gravity measurements with accuracy 1 mGal are required. Horizontal accelerations in the surge and sway directions are suppressed about 60 dB in the frequency range 0...
June 2017: Review of Scientific Instruments
https://www.readbyqxmd.com/read/28666228/high-throughput-quantification-of-8-antihypertensive-drugs-and-active-metabolites-in-human-plasma-using-uplc-ms-ms
#10
Bart C H van der Nagel, Jorie Versmissen, Soma Bahmany, Teun van Gelder, Birgit C P Koch
BACKGROUND: To assess drug adherence of patients with hypertension, an analytical method was developed and validated using ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). The method includes eight frequently prescribed antihypertensive drugs from four classes and their active metabolites: angiotensin converting enzyme inhibitors enalapril and perindopril (active metabolites respectively enalaprilate and perindoprilate), angiotensin II receptor blockers losartan (with the active metabolite losartan carboxylic acid) and valsartan, calcium channel blockers amlodipine and nifedipine and diuretics hydrochlorothiazide and spironolactone (with the active metabolite canrenone)...
June 19, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28646303/three-dimensional-fine-structure-of-the-organization-of-microtubules-in-neurite-varicosities-by-ultra-high-voltage-electron-microscope-tomography
#11
Tomoki Nishida, Ryoichi Yoshimura, Yasuhisa Endo
Neurite varicosities are highly specialized compartments that are involved in neurotransmitter/ neuromodulator release and provide a physiological platform for neural functions. However, it remains unclear how microtubule organization contributes to the form of varicosity. Here, we examine the three-dimensional structure of microtubules in varicosities of a differentiated PC12 neural cell line using ultra-high voltage electron microscope tomography. Three-dimensional imaging showed that a part of the varicosities contained an accumulation of organelles that were separated from parallel microtubule arrays...
June 23, 2017: Cell and Tissue Research
https://www.readbyqxmd.com/read/28634757/a-uhplc-ms-ms-method-for-profiling-multifunctional-steroids-in-human-hair
#12
Zhen Dong, Caihong Wang, Jinlan Zhang, Zhe Wang
It is important to profile steroids in many physiological and pathological processes. Recently, hair has been used for the long-term measurement of endogenous steroid hormones. Analyzing hair has advantages of being noninvasive and time sequential compared with other bio-specimens. Liquid chromatography-mass spectrometry (LC-MS) techniques have been widely used over the past decades; however, it is challenging to profile estrogens in hair by LC-MS, and more comprehensive steroid profiling is required. In this paper, an ultra high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed to simultaneously profile 28 multifunctional steroids, including corticosteroids (n = 6), estrogens (n = 13), androgens (n = 5) and progestogens (n = 4), in human scalp hair in a single run...
June 20, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28619585/sensitivity-enhancement-by-chromatographic-peak-concentration-with-ultra-high-performance-liquid-chromatography-nuclear-magnetic-resonance-spectroscopy-for-minor-impurity-analysis
#13
Takashi Tokunaga, Ken-Ichi Akagi, Masahiko Okamoto
High performance liquid chromatography can be coupled with nuclear magnetic resonance (NMR) spectroscopy to give a powerful analytical method known as liquid chromatography-nuclear magnetic resonance (LC-NMR) spectroscopy, which can be used to determine the chemical structures of the components of complex mixtures. However, intrinsic limitations in the sensitivity of NMR spectroscopy have restricted the scope of this procedure, and resolving these limitations remains a critical problem for analysis. In this study, we coupled ultra-high performance liquid chromatography (UHPLC) with NMR to give a simple and versatile analytical method with higher sensitivity than conventional LC-NMR...
June 9, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28603183/uplc-ms-ms-analysis-of-methotrexate-in-human-plasma-and-comparison-with-the-fluorescence-polarization-immunoassay
#14
Shenghui Mei, Leting Zhu, Xingang Li, Jiaqing Wang, Xueyun Jiang, Haiyan Chen, Jiping Huo, Li Yang, Song Lin, Zhigang Zhao
Methotrexate (MTX) plasma concentration is routinely monitored to guide the dosage regimen of rescue drugs. This study aims to develop and validate an ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for plasma MTX analysis, and to establish its agreement with the fluorescence polarization immunoassay (FPIA) in patients with high-dose MTX therapy. Separation was achieved by gradient elution with methanol and water (0.05% formic acid) at 40°C with a run time of 3 min. The intra- and inter-day inaccuracy and imprecision of the UPLC-MS/MS method were -4...
2017: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/28600963/ultra-performance-liquid-chromatography-tandem-mass-spectrometry-based-multiplex-enzyme-assay-for-six-enzymes-associated-with-hereditary-hemolytic-anemia
#15
Chul Min Park, Kyunghoon Lee, Sun-Hee Jun, Sang Hoon Song, Junghan Song
Deficiencies in erythrocyte metabolic enzymes are associated with hereditary hemolytic anemia. Here, we report the development of a novel multiplex enzyme assay for six major enzymes, namely glucose-6-phosphate dehydrogenase, pyruvate kinase, pyrimidine 5'-nucleotidase, hexokinase, triosephosphate isomerase, and adenosine deaminase, deficiencies in which are implicated in erythrocyte enzymopathies. To overcome the drawbacks of traditional spectrophotometric enzyme assays, the present assay was based on ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)...
June 3, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28598602/multiactinide-analysis-with-accelerator-mass-spectrometry-for-ultratrace-determination-in-small-samples-application-to-an-in-situ-radionuclide-tracer-test-within-the-colloid-formation-and-migration-experiment-at-the-grimsel-test-site-switzerland
#16
Francesca Quinto, Ingo Blechschmidt, Carmen Garcia Perez, Horst Geckeis, Frank Geyer, Robin Golser, Florian Huber, Markus Lagos, Bill Lanyon, Markus Plaschke, Peter Steier, Thorsten Schäfer
The multiactinide analysis with accelerator mass spectrometry (AMS) was applied to samples collected from the run 13-05 of the Colloid Formation and Migration (CFM) experiment at the Grimsel Test Site (GTS). In this in situ radionuclide tracer test, the environmental behavior of (233)U, (237)Np, (242)Pu, and (243)Am was investigated in a water conductive shear zone under conditions relevant for a nuclear waste repository in crystalline rock. The concentration of the actinides in the GTS groundwater was determined with AMS over 6 orders of magnitude from ∼15 pg/g down to ∼25 ag/g...
June 16, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28583068/lncrna-screen-an-interactive-platform-for-computationally-screening-long-non-coding-rnas-in-large-genomics-datasets
#17
Yixiao Gong, Hsuan-Ting Huang, Yu Liang, Thomas Trimarchi, Iannis Aifantis, Aristotelis Tsirigos
BACKGROUND: Long non-coding RNAs (lncRNAs) have emerged as a class of factors that are important for regulating development and cancer. Computational prediction of lncRNAs from ultra-deep RNA sequencing has been successful in identifying candidate lncRNAs. However, the complexity of handling and integrating different types of genomics data poses significant challenges to experimental laboratories that lack extensive genomics expertise. RESULT: To address this issue, we have developed lncRNA-screen, a comprehensive pipeline for computationally screening putative lncRNA transcripts over large multimodal datasets...
June 5, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28578567/quantitative-profiling-of-endogenous-fat-soluble-vitamins-and-carotenoids-in-human-plasma-using-an-improved-uhpsfc-esi-ms-interface
#18
Filomena Petruzziello, Alexandre Grand-Guillaume Perrenoud, Anita Thorimbert, Michael Fogwill, Serge Rezzi
Analytical solutions enabling the quantification of circulating levels of liposoluble micronutrients such as vitamins and carotenoids are currently limited to either single or a reduced panel of analytes. The requirement to use multiple approaches hampers the investigation of the biological variability on a large number of samples in a time and cost efficient manner. With the goal to develop high-throughput and robust quantitative methods for the profiling of micronutrients in human plasma, we introduce a novel, validated workflow for the determination of 14 fat-soluble vitamins and carotenoids in a single run...
June 27, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28558416/fast-ultra-high-performance-liquid-chromatography-with-diode-array-and-mass-spectrometry-method-for-determination-of-tadalafil-drug-substance-and-its-impurities
#19
Ludovit Schreiber, Radoslav Halko, Milan Hutta
Tadalafil is used for the treatment of erectile dysfunction and its related patents expired in 2016, therefore related generic drugs production is supposed to be increased. This work is focused on developing a fast ultra high performance liquid chromatography (UHPLC) with diode array detector (DAD) and/or mass spectrometry (MS) detection for the separation and determination of tadalafil and its impurities in pharmaceutical samples. Modern reversed-phase stationary phase with sub-2 micron particle size, Zorbax StableBond Rapid Resolution High Definition with octylsilane chemically bonded phase to totally porous silica particles was used for the solving this problem...
May 30, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28553940/single-cell-genome-sequencing-at-ultra-high-throughput-with-microfluidic-droplet-barcoding
#20
Freeman Lan, Benjamin Demaree, Noorsher Ahmed, Adam R Abate
The application of single-cell genome sequencing to large cell populations has been hindered by technical challenges in isolating single cells during genome preparation. Here we present single-cell genomic sequencing (SiC-seq), which uses droplet microfluidics to isolate, fragment, and barcode the genomes of single cells, followed by Illumina sequencing of pooled DNA. We demonstrate ultra-high-throughput sequencing of >50,000 cells per run in a synthetic community of Gram-negative and Gram-positive bacteria and fungi...
July 2017: Nature Biotechnology
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