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Fashui Hong, Yuguan Ze, Yaoming Zhou, Jie Hong, Xiaohon Yu, Lei Sheng, Ling Wang
Titanium dioxide nanoparticles (TiO2 NPs) are increasingly used in daily life, in industry, and in environmental clearing, but their potential neurodevelopmental toxicity has been highly debated. In this study, we explored whether TiO2 NPs inhibited development of dendritic morphology and identified possible molecular mechanisms associated with this inhibition in primary cultured rat hippocampal neurons. Results showed that TiO2 NPs decreased neurite length, the number of branches and the spine density, and impaired mitochondrial function in the developing neurons...
March 28, 2017: Journal of Biomedical Materials Research. Part A
Tam T Quach, Sarah M Wilson, Veronique Rogemond, Naura Chounlamountri, Pappachan E Kolattukudy, Stephanie Martinez, May Khanna, Marie-Francoise Belin, Rajesh Khanna, Jerome Honnorat, Anne-Marie Duchemin
Although hippocampal neurons are well-distinguished by the morphological characteristics of their dendrites and their structural plasticity, the mechanisms involved in regulating their neurite initiation, dendrite growth, network formation and remodeling are still largely unknown, in part because the key molecules involved remain elusive. Identifying new dendrite-active cues could uncover unknown molecular mechanisms that would add significant understanding to the field and possibly lead to the development of novel neuroprotective therapy because these neurons are impaired in many neuropsychiatric disorders...
September 15, 2013: Journal of Cell Science
Sheng T Hou, Susan X Jiang, Amy Aylsworth, Matthew Cooke, Lei Zhou
CRMP proteins play critical regulatory roles during semaphorin-mediated neurite outgrowth, neuronal differentiation and death. Albeit having a high degree of structure and sequence resemblance to that of liver dihydropyrimidinase, purified rodent brain CRMPs do not hydrolyze dihydropyrimidinase substrates. Here we found that mouse CRMP3 has robust histone H4 deacetylase activity. During excitotoxicity-induced mouse neuronal death, calpain-cleaved, N-terminally truncated CRMP3 undergoes nuclear translocation to cause nuclear condensation through deacetylation of histone H4...
2013: Scientific Reports
Yanming Chen, Hui Sheng, Yongjun Xu, Yanmin Zhang, Xin Ni
Corticotropin-releasing hormone (CRH) family peptides as well as their receptors have been shown to exhibit various functions in hippocampus. However, effects of CRH receptors activation on collapsin response mediator protein 3 (CRMP3), the key protein for dendrite outgrowth and cell apoptosis, remain unclear. In the present study, we determined the effects of CRHR1 and CRHR2 on CRMP3 expression in cultured hippocampal neurons. CRH and urocortin II (UCNII) dose-dependently suppressed CRMP3 mRNA and protein expression...
April 2012: Neuropeptides
Bruno Douradinha, Kevin D Augustijn, Sally G Moore, Jai Ramesar, Maria M Mota, Andrew P Waters, Chris J Janse, Joanne Thompson
BACKGROUND: The Plasmodium Cysteine Repeat Modular Proteins (PCRMP) are a family of four conserved proteins of malaria parasites, that contain a number of motifs implicated in host-parasite interactions. Analysis of mutants of the rodent parasite Plasmodium berghei lacking expression of PCRMP1 or 2 showed that these proteins are essential for targeting of P. berghei sporozoites to the mosquito salivary gland and, hence, for transmission from the mosquito to the mouse. METHODS: In this work, the role of the remaining PCRMP family members, PCRMP3 and 4, has been investigated throughout the Plasmodium life cycle by generation and analysis of P...
March 31, 2011: Malaria Journal
Amy Aylsworth, Susan X Jiang, Angele Desbois, Sheng T Hou
Collapsin response mediator proteins (CRMPs) are key modulators of cytoskeletons during neurite outgrowth in response to chemorepulsive guidance molecules. However, their roles in adult injured neurons are not well understood. We previously demonstrated that CRMP3 underwent calcium-dependent N-terminal protein cleavage during excitotoxicity-induced neurite retraction and neuronal death. Here, we report findings that the full-length CRMP3 inhibits tubulin polymerization and neurite outgrowth in cultured mature cerebellar granule neurons, while the N-terminal truncated CRMP3 underwent nuclear translocation and caused a significant nuclear condensation...
October 1, 2009: Experimental Cell Research
Tam T Quach, Guy Massicotte, Marie-Françoise Belin, Jérome Honnorat, Erica R Glasper, Anne C Devries, Lyn B Jakeman, Michel Baudry, Anne-Marie Duchemin, Pappachan E Kolattukudy
In vitro studies have pointed to the collapsin response mediator proteins (CRMPs) as key regulators of neurite outgrowth and axonal differentiation. CRMP3 is expressed mostly in the nervous system during development but remains at high levels in the hippocampus of adults. To explore CRMP3 function in vivo, we generated mice with targeted disruption of the CRMP3 gene. Immunohistochemistry and Golgi staining of CA1 showed abnormal dendrite and spine morphogenesis in the hippocampus of CRMP3-deficient mice. Apical dendrites displayed an increase in undulation and a reduction in length and branching points...
February 2008: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Susan X Jiang, Joachim Kappler, Bogdan Zurakowski, Angele Desbois, Amy Aylsworth, Sheng T Hou
Collapsin response mediator proteins (CRMPs) are important brain-specific proteins with distinct functions in modulating growth cone collapse and axonal guidance during brain development. Our previous studies have shown that calpain cleaves CRMP3 in the adult mouse brain during cerebral ischemia [S.T. Hou et al. (2006) J. Neurosci., 26, 2241-2249]. Here, the expression of all CRMP family members (1-5) was examined in mouse brains that were subjected to middle cerebral artery occlusion. Among the five CRMPs, the expressions of CRMP1, CRMP3 and CRMP5 were the most abundant in the cerebral cortex and all CRMPs were targeted for cleavage by ischemia-activated calpain...
August 2007: European Journal of Neuroscience
A Knudsen, G Bredholt, A Storstein, L Oltedal, S Davanger, B Krossnes, J Honnorat, C A Vedeler
We present a case with subacute limbic encephalitis (LE) and thymoma. Neither classical onconeural antibodies nor antibodies to voltage gated potassium channels (VGKC) were detected, but the serum was positive for anti-glutamic acid decarboxylase (GAD). The patient serum also stained synaptic boutons of pyramidal cells and nuclei of granule cells of rat hippocampus. The objective of the study was to identify new antibodies associated with LE. Screening a cDNA expression library identified collapsin response mediator protein 3 (CRMP3), a protein involved in neurite outgrowth...
July 2007: Clinical and Experimental Immunology
Lieselotte Cnops, Babs Van de Plas, Lutgarde Arckens
The functional properties and anatomical organization of the mammalian visual cortex are immature at birth and develop gradually during the first postnatal weeks. There is a 'critical period' where the cortex is plastic and susceptible to changes in visual input. Knowledge of proteins with a high expression during this period has great importance for the understanding of activity-driven maturation of the brain. The collapsin response mediator protein family consists of five cytosolic phosphoproteins (CRMP1-5) that are involved in neuronal differentiation during the development of the nervous system...
April 2004: European Journal of Neuroscience
D Ricard, B Stankoff, D Bagnard, M Aguera, V Rogemond, J C Antoine, N Spassky, B Zalc, C Lubetzki, M F Belin, J Honnorat
The family of collapsin response mediator protein/Unc-33-like protein (CRMP/Ulip), composed of four homologous members, is specifically and highly expressed in the nervous system during embryonic neuronal development and dramatically down-regulated in the adult. Members of this family have been proposed to be part of the semaphorins signal transduction pathway involved in axonal outgrowth. Here, we show by in situ hybridization and immunohistochemistry that CRMP2/Ulip2, and to a lesser extent CRMP3/Ulip4, are expressed in immature and mature oligodendrocytes, but not in astrocytes...
October 2000: Molecular and Cellular Neurosciences
R Inatome, T Tsujimura, T Hitomi, N Mitsui, P Hermann, S Kuroda, H Yamamura, S Yanagi
Four members of collapsin response mediator proteins (CRMPs) are thought to be involved in the semaphorin-induced growth cone collapse during neural development. Here we report the identification of a novel CRMP3-associated protein, designated CRAM for CRMP3-associated molecule, that belongs to the unc-33 gene family. The deduced amino acid sequence reveals that the CRAM gene encodes a protein of 563 amino acids, shows 57% identity with dihydropyrimidinase, and shows 50-51% identity with CRMPs. CRAM appears to form a large complex composed of CRMP3 and other unidentified proteins in vivo...
September 1, 2000: Journal of Biological Chemistry
T T Quach, B Mosinger, D Ricard, N G Copeland, D J Gilbert, N A Jenkins, B Stankoff, J Honnorat, M F Belin, P Kolattukudy
CRMPs (collapsin response mediator proteins)/ULIPs (unc-33-like proteins) are a family of intracytoplasmic proteins that are expressed mainly in the brain. The involvement of CRMP/ULIP members in neuronal differentiation, growth cone motility and axonal collapse has been suggested. We recently found that a member of this family, CRMP3/ULIP4, corresponds to POP66 (paraneoplastic oligodendrocyte protein of 66 kDa), a protein which may be associated with auto-immune induced-neuronal degeneration in paraneoplastic neurological syndromes...
January 25, 2000: Gene
J Honnorat, T Byk, I Kusters, M Aguera, D Ricard, V Rogemond, T Quach, D Aunis, A Sobel, M G Mattei, P Kolattukudy, M F Belin, J C Antoine
Anti-CV2 autoantibodies have recently been discovered in patients with paraneoplastic neurological diseases (PND). These disorders are associated with neuronal degeneration, mediated by autoimmune processes, in patients with systemic cancer. Anti-CV2 autoantibodies recognize a brain protein of 66 kDa developmentally regulated and specifically expressed by a subpopulation of oligodendrocytes in the adult brain. Here, we demonstrate that anti-CV2 sera recognize several post-translationally modified forms of Ulip4/CRMP3, a member of a protein family related to the axonal guidance and homologous to the Unc-33 gene product in Caenorhabditis elegans...
December 1999: European Journal of Neuroscience
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