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https://www.readbyqxmd.com/read/29145642/arf2-4-and-arf5-are-essential-for-female-and-male-gametophyte-development-in-arabidopsis
#1
Zhenning Liu, Liming Miao, Ruxue Huo, Xiaoya Song, Johnson Cameron, Lijun Kong, Venkatesan Sundaresan, Xiaolin Yu
The plant hormone auxin plays critical roles in plant growth and development. Auxin response factors (ARFs) are a class of transcription factors which regulate auxin-mediated gene expression. While the functions of ARFs in sporophytic development have been well characterized, their functions specific to gametophytic development have not been studied extensively. In this study, Arabidopsis ARF genes were selectively down-regulated in gametophytes by mis-expression of targeted microRNAs (amiRARF234, amiRARFMP and MIR167a) to silence AtARF2-4, AtARF5, AtARF6 and AtARF8...
November 14, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/29144434/the-role-of-non-coding-rnas-in-cytoplasmic-male-sterility-in-flowering-plants
#2
REVIEW
Helena Štorchová
The interactions between mitochondria and nucleus substantially influence plant development, stress response and morphological features. The prominent example of a mitochondrial-nuclear interaction is cytoplasmic male sterility (CMS), when plants produce aborted anthers or inviable pollen. The genes responsible for CMS are located in mitochondrial genome, but their expression is controlled by nuclear genes, called fertility restorers. Recent explosion of high-throughput sequencing methods enabled to study transcriptomic alterations in the level of non-coding RNAs under CMS biogenesis...
November 16, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29144196/quantitative-weight-of-evidence-assessment-of-higher-tier-studies-on-the-toxicity-and-risks-of-neonicotinoids-in-honeybees-3-clothianidin
#3
Keith R Solomon, Gladys L Stephenson
A quantitative weight of evidence (QWoE) methodology was used to assess higher tier studies on the effects of clothianidin (CTD) on honeybees. Assessment endpoints were population size and viability of commercially managed bees and quantity of hive products. A colony-level no-observed-adverse effect concentration (NOAEC) of 25 µg CTD/kg syrup, equivalent to an oral no-observed-adverse effect-dose (NOAED) of 7.3 ng/bee/d for all responses measured. Based on a NOAEC of 19.7 µg/kg pollen, the NOAED for honeybee larvae was 2...
November 16, 2017: Journal of Toxicology and Environmental Health. Part B, Critical Reviews
https://www.readbyqxmd.com/read/29143737/validation-of-elisa-methods-for%C3%A2-quantification-of-the-major-birch-allergen-bet-v-1-bsp090
#4
S Kaul, J Zimmer, O Dehus, A Constanzo, A DaasConstanzo, K-H Buchheit, J Asturias, M C Arilla, D Barber, A Bertocchi, B Brunetto, J A Carnes, M Chapman, G Chaudemanche, J Dayan-Kenigsberg, S Döring, F Führer, M T Gallego, P Iacovacci, K M Hanschmann, T Holzhauser, M Hrabina, A Ledesma, P Moingeon, E Nony, C Pini, G Plunkett, M Raulf, G Reese, E Sandberg, I Sander, B Smith, D Strecker, C Valerio, R van Ree, B Weber, S Vieths
To date, the potency of allergen products in Europe is expressed in manufacturer-specific units relative to a product-specific in-house reference. Consequently, cross-product comparability of allergen products from different manufacturers with respect to strength and efficacy is impossible. The Biological Standardisation Programme (BSP) project BSP090 addresses this issue via the establishment of reference standards in conjunction with ELISA methods for the quantification of major allergens in allergen products...
2017: Pharmeuropa Bio & Scientific Notes
https://www.readbyqxmd.com/read/29143171/neonicotinoid-insecticides-in-pollen-honey-and-adult-bees-in-colonies-of-the-european-honey-bee-apis-mellifera-l-in-egypt
#5
Garry Codling, Yahya Al Naggar, John P Giesy, Albert J Robertson
Honeybee losses have been attributed to multiple stressors and factors including the neonicotinoid insecticides (NIs). Much of the study of hive contamination has been focused upon temperate regions such as Europe, Canada and the United States. This study looks for the first time at honey, pollen and bees collected from across the Nile Delta in Egypt in both the spring and summer planting season of 2013. There is limited information upon the frequency of use of NIs in Egypt but the ratio of positive identification and concentrations of NIs are comparable to other regions...
November 15, 2017: Ecotoxicology
https://www.readbyqxmd.com/read/29141708/sensitive-detection-of-neonicotinoid-insecticides-and-other-selected-pesticides-in-pollen-and-nectar-using-nanoflow-liquid-chromatography-orbitrap-tandem-mass-spectrometry
#6
David Moreno-González, Jaime Alcántara-Durán, Bienvenida Gilbert-López, Miriam Beneito-Cambra, Víctor M Cutillas, Łukasz Rajski, Antonio Molina-Díaz, Juan F García-Reyes
In this work, a new method based on nanoflow LC with high-resolution MS was developed for the determination ofeight pesticides in pollen and nectar samples, including neonicotinoid insecticides and other selected pesticides commonly found in bees and beeswax. Detection was undertaken with a hybrid quadrupole-Orbitrap mass spectrometer (Q Exactive™) equipped with a commercial nanospray ion source. The extraction of pesticides from pollen samples was performed by a modified micro-QuEChERS method scaled down to Eppendorf tubes, whereas nectar samples were simply diluted with a water–methanol (95 + 5, v/v) solution...
November 15, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/29141565/radio-modulatory-potential-ofcaffeic-acid-phenethyl-ester-a-therapeutic-perspective
#7
Km Anjaly, Ashu Tiku
Use of natural agents is an upcoming area of research in cancer biology. Caffeic acid phenethyl ester has received great attention because of its therapeutic potential in various conditions including cancer. It is an active/abundant component of propolis. Propolis is a honey bee hive product produced by bees using their enzyme-rich digestive secretions on resinous mix, bee wax and pollen from plants. It is used to protect the beehive against bacteria and other infections.Although a lot of work has been done on chemotherapeutic aspects of CAPE, its role as a radiomodulator is yet to be delineated...
November 13, 2017: Anti-cancer Agents in Medicinal Chemistry
https://www.readbyqxmd.com/read/29140765/quantitative-weight-of-evidence-assessment-of-higher-tier-studies-on-the-toxicity-and-risks-of-neonicotinoids-in-honeybees-2-imidacloprid
#8
Gladys L Stephenson, Keith R Solomon
A quantitative weight of evidence (QWoE) methodology was used to assess higher-tier studies on the effects of imidacloprid (IMI) on honeybees. Assessment endpoints were population size and viability of commercially managed bees and quantity of hive products. A colony-level no-observed-adverse effect concentration (NOAEC) of 25 µg IMI/kg syrup, equivalent to an oral no-observed-adverse-effect-dose of 7.3 ng/bee/d for all responses, was measured. The overall weight of evidence indicates that there is minimal risk to honeybees from exposure to IMI from its use as a seed treatment...
November 15, 2017: Journal of Toxicology and Environmental Health. Part B, Critical Reviews
https://www.readbyqxmd.com/read/29138404/neonicotinoid-pesticide-limits-improvement-in-buzz-pollination-by-bumblebees
#9
P R Whitehorn, C Wallace, M Vallejo-Marin
Neonicotinoid pesticides have been linked to global declines of beneficial insects such as bumblebees. Exposure to trace levels of these chemicals causes sub-lethal effects, such as reduced learning and foraging efficiency. Complex behaviours may be particularly vulnerable to the neurotoxic effects of neonicotinoids. Such behaviours may include buzz pollination (sonication), in which pollinators, usually bees, use innate and learned behaviours to generate high-frequency vibrations to release pollen from flowers with specialised anther morphologies...
November 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29135514/prevalence-and-clinical-characteristics-of-local-allergic-rhinitis-to-house-dust-mites
#10
Yoo Seob Shin, Chang-Gyu Jung, Hae-Sim Park
PURPOSE OF REVIEW: Local allergic rhinitis (LAR) is a recently classified subtype of rhinitis defined by a nasal allergic response in patients without systemic evidence of atopy. Recent studies have reported the prevalence, clinical course, culprit allergens, diagnostic methods and treatment outcomes of LAR. The purpose of this review is to summarize the most relevant and updated scientific evidence for LAR, especially focusing on its prevalence and clinical characteristics. RECENT FINDINGS: LAR is found in a significant proportion (3...
November 14, 2017: Current Opinion in Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29134726/the-divergence-history-of-the-perennial-plant-linaria-cavanillesii-confirms-a-recent-loss-of-self-incompatibility
#11
Marie Voillemot, Quentin Rougemont, Camille Roux, John R Pannell
Many angiosperms prevent inbreeding through a self-incompatibility (SI) system, but the loss of SI has been frequent in their evolutionary history. The loss of SI may often lead to an increase in the selfing rate, with the purging of inbreeding depression and the ultimate evolution of a selfing syndrome, where plants have smaller flowers with reduced pollen and nectar production. In this study, we used approximate Bayesian computation (ABC) to estimate the timing of divergence between populations of the plant Linaria cavanillesii, whose populations differ in SI status and in which SI is associated with low inbreeding depression but not with a transition to full selfing or a selfing syndrome...
November 13, 2017: Journal of Evolutionary Biology
https://www.readbyqxmd.com/read/29134400/safe-sites-of-pollen-placement-a-conflict-of-interest-between-plants-and-bees
#12
Ze-Yu Tong, Shuang-Quan Huang
Plant stigmas and bee pollinators are competitors for pollen. Pollen placed on a pollinator's body can be picked up by conspecific stigmas or it can be collected by the pollinator as food. Hypothetically, one solution is for pollen to be placed on 'safe sites' on the pollinator's body, sites where the pollinator cannot easily remove it, leaving the pollen for stigmas. We compared 14 sites on the bumblebee body in terms of the ability of the bee to groom off fluorescent power, a dust that may be thought of as analogous to pollen...
November 13, 2017: Oecologia
https://www.readbyqxmd.com/read/29134290/re-emergence-of-lilium-callosum-sieb-et-zucc-in-taiwan-after-a-fire-allows-propagation-and-renews-the-possibility-of-conservation
#13
Ying Chun Chen, Yang Jung Huang, Chiu Mei Wang, Chin An Chiu, Huey Ling Lin, Pei Fang Lee, Ya Ming Cheng, Chen Chang
BACKGROUND: Lilium callosum is native to Taiwan, but little is known about it since it has been considered extinct since 1915. After the rediscovery of this rare species after a fire in 2011 in Tunghsiao Township, intensive work has been conducted to count the number in the wild population, to develop a conservation strategy, and to understand its reproductive characteristics and even economic potential. RESULTS: To conserve the germplasm of this population, three scales from a wild L...
November 13, 2017: Botanical Studies (Taipei, Taiwan)
https://www.readbyqxmd.com/read/29134282/aba-and-iaa-control-microsporogenesis-in-petunia-hybrida-l
#14
L V Kovaleva, A S Voronkov, E V Zakharova, I M Andreev
The formation of fertile male gametophyte is known to require timely degeneration of polyfunctional tapetum tissue. The last process caused by the programmed cell death (PCD) is a part of the anther program maturation which leads to sequential anther tissue destruction coordinated with pollen differentiation. In the present work, distribution of abscisic acid (ABA) and indole-3-acetic acid (IAA) in developing anthers of male-fertile and male-sterile lines of petunia (Petunia hybrida L.) was analyzed by using the immunohistochemical method...
November 13, 2017: Protoplasma
https://www.readbyqxmd.com/read/29134240/fine-mapping-of-a-male-sterility-gene-ms-3-in-a-novel-cucumber-cucumis-sativus-l-mutant
#15
Yike Han, Fengyue Zhao, Shang Gao, Xianyun Wang, Aimin Wei, Zhengwu Chen, Nan Liu, Xueqiang Tong, Xinmeng Fu, Changlong Wen, Zhenxian Zhang, Ningning Wang, Shengli Du
The cucumber male sterility gene ms - 3 was fine mapped in a 76 kb region harboring an MMD1 -like gene Csa3M006660 that may be responsible for the male sterile in cucumber. A cucumber (Cucumis sativus L.) male sterile mutant (ms-3) in an advanced-generation inbred line was identified, and genetic analysis revealed that the male sterility trait was controlled by a recessive nuclear gene, ms-3, which was stably inherited. Histological studies suggested that the main cause of the male sterility was defective microsporogenesis, resulting in no tetrad or microspores being formed...
November 13, 2017: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/29133421/early-neolithic-wine-of-georgia-in-the-south-caucasus
#16
Patrick McGovern, Mindia Jalabadze, Stephen Batiuk, Michael P Callahan, Karen E Smith, Gretchen R Hall, Eliso Kvavadze, David Maghradze, Nana Rusishvili, Laurent Bouby, Osvaldo Failla, Gabriele Cola, Luigi Mariani, Elisabetta Boaretto, Roberto Bacilieri, Patrice This, Nathan Wales, David Lordkipanidze
Chemical analyses of ancient organic compounds absorbed into the pottery fabrics from sites in Georgia in the South Caucasus region, dating to the early Neolithic period (ca. 6,000-5,000 BC), provide the earliest biomolecular archaeological evidence for grape wine and viniculture from the Near East, at ca. 6,000-5,800 BC. The chemical findings are corroborated by climatic and environmental reconstruction, together with archaeobotanical evidence, including grape pollen, starch, and epidermal remains associated with a jar of similar type and date...
November 13, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29133226/the-effect-of-dietary-neonicotinoid-pesticides-on-non-flight-thermogenesis-in-worker-bumble-bees-bombus-terrestris
#17
Robert Potts, Rebecca M Clarke, Sophie E Oldfield, Lisa K Wood, Natalie Hempel de Ibarra, James E Cresswell
For bumble bees (genus Bombus), the capacity for non-flight thermogenesis is essential for two fundamental processes undertaken by adult workers, namely recovery from torpor after chilling and brood incubation. Farmland bees can be widely exposed to dietary residues of neurotoxic neonicotinoid insecticides that appear in the nectar and pollen of treated bee-attractive crops, which may harm them. An earlier study shows that dietary neonicotinoids cause complex alterations to thermoregulation in honey bees, but their effects on the thermogenic capabilities of individual bumble bees has been untested previously...
November 10, 2017: Journal of Insect Physiology
https://www.readbyqxmd.com/read/29132467/-distribution-characteristics-of-serum-specific-ige-for-inhaled-allergens-in-children-with-different-airway-allergic-diseases
#18
Kang Zhu, Xiao-Ling Hou, Hui-Jie Huang, Ya-Ru Wang, Yi-Xin Ren, Xin Ni, Li Xiang
OBJECTIVE: To investigate the distribution characteristics of serum specific IgE (sIgE) for inhaled allergens in children with different airway allergic diseases. METHODS: Fluorescent enzyme-linked immunosorbent assay on the UniCAP250 system was performed to measure serum sIgE for 9 common inhaled allergens in 256 children aged 3-14 years with different airway allergic diseases. According to the clinical diagnosis, these children were divided into rhinitis group (37 children with allergic rhinitis), asthma group (82 children with bronchial asthma), and rhinitis-asthma group (137 children with allergic rhinitis complicated by bronchial asthma)...
November 2017: Zhongguo Dang Dai Er Ke za Zhi, Chinese Journal of Contemporary Pediatrics
https://www.readbyqxmd.com/read/29131847/evolutionary-history-of-callose-synthases-in-terrestrial-plants-with-emphasis-on-proteins-involved-in-male-gametophyte-development
#19
Lenka Záveská Drábková, David Honys
Callose is a plant-specific polysaccharide (β-1,3-glucan) playing an important role in angiosperms in many developmental processes and responses to biotic and abiotic stresses. Callose is synthesised at the plasma membrane of plant cells by callose synthase (CalS) and, among others, represents the main polysaccharide in the callose wall surrounding the tetrads of developing microspores and in the growing pollen tube wall. CalS proteins involvement in spore development is a plesiomorphic feature of terrestrial plants, but very little is known about their evolutionary origin and relationships amongst the members of this protein family...
2017: PloS One
https://www.readbyqxmd.com/read/29130803/steroid-sparing-effect-of-sublingual-immunotherapy-real-life-study-in-mono-polisensitized-children-with-asthma
#20
Nerin Nadir Bahceciler, Nilufer Galip, Arzu Babayigit
AIM: Steroid-sparing effect of sublingual immunotherapy (SLIT) in mono/polisensitized asthmatic children were evaluated. MATERIALS AND METHODS:  Children undergoing allergen-specific SLIT between 2010 and 2014 were included. Asthma control and usage/dose of inhaled corticosteroid (ICS) in the previous year was determined. Asthma control without ICS need ≥6 months was defined as 'ICS avoidance'. RESULTS:  90 children (mean ± SD age 8...
November 2017: Immunotherapy
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