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https://www.readbyqxmd.com/read/28211906/dynamics-of-male-meiotic-recombination-frequency-during-plant-development-using-fluorescent-tagged-lines-in-arabidopsis-thaliana
#1
Fan Li, Nico De Storme, Danny Geelen
Meiotic homologous recombination plays a central role in creating genetic variability, making it an essential biological process relevant to evolution and crop breeding. In this study, we used pollen-specific fluorescent tagged lines (FTLs) to measure male meiotic recombination frequency during the development of Arabidopsis thaliana. Interestingly, a subset of pollen grains consistently shows loss of fluorescence expression in tested lines. Using nine independent FTL intervals, the spatio-temporal dynamics of male recombination frequency was assessed during plant development, considering both shoot type and plant age as independent parameters...
February 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28208095/relation-between-modern-pollen-rain-vegetation-and-climate-in-northern-china-implications-for-quantitative-vegetation-reconstruction-in-a-steppe-environment
#2
Yawen Ge, Yuecong Li, M Jane Bunting, Bing Li, Zetao Li, Junting Wang
Vegetation reconstructions from palaeoecological records depend on adequate understanding of relationships between modern pollen, vegetation and climate. A key parameter for quantitative vegetation reconstructions is the Relative Pollen Productivity (RPP). Differences in both environmental and methodological factors are known to alter the RPP estimated significantly, making it difficult to determine whether the underlying pollen productivity does actually vary, and if so, why. In this paper, we present the results of a replication study for the Bashang steppe region, a typical steppe area in northern China, carried out in 2013 and 2014...
February 13, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28205672/alternative-splicing-in-tomato-pollen-in-response-to-heat-stress%C3%A2
#3
Mario Keller, Yangjie Hu, Anida Mesihovic, Sotirios Fragkostefanakis, Enrico Schleiff, Stefan Simm
No abstract text is available yet for this article.
December 24, 2016: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes
https://www.readbyqxmd.com/read/28205632/using-dna-metabarcoding-to-investigate-honey-bee-foraging-reveals-limited-flower-use-despite-high-floral-availability
#4
Natasha de Vere, Laura E Jones, Tegan Gilmore, Jake Moscrop, Abigail Lowe, Dan Smith, Matthew J Hegarty, Simon Creer, Col R Ford
Understanding which flowers honey bees (Apis mellifera) use for forage can help us to provide suitable plants for healthy honey bee colonies. Accordingly, honey DNA metabarcoding provides a valuable tool for investigating pollen and nectar collection. We investigated early season (April and May) floral choice by honey bees provided with a very high diversity of flowering plants within the National Botanic Garden of Wales. There was a close correspondence between the phenology of flowering and the detection of plants within the honey...
February 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28204486/migratory-bee-hive-transportation-contributes-insignificantly-to-transgenic-pollen-movement-between-spatially-isolated-alfalfa-seed-fields
#5
Natalie K Boyle, Sandya R Kesoju, Stephanie L Greene, Ruth C Martin, Douglas B Walsh
No abstract text is available yet for this article.
February 1, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/28202726/two-tonoplast-mate-proteins-function-as-turgor-regulating-chloride-channels-in-arabidopsis
#6
Haiwen Zhang, Fu-Geng Zhao, Ren-Jie Tang, Yuexuan Yu, Jiali Song, Yuan Wang, Legong Li, Sheng Luan
The central vacuole in a plant cell occupies the majority of the cellular volume and plays a key role in turgor regulation. The vacuolar membrane (tonoplast) contains a large number of transporters that mediate fluxes of solutes and water, thereby adjusting cell turgor in response to developmental and environmental signals. We report that two tonoplast Detoxification efflux carrier (DTX)/Multidrug and Toxic Compound Extrusion (MATE) transporters, DTX33 and DTX35, function as chloride channels essential for turgor regulation in Arabidopsis Ectopic expression of each transporter in Nicotiana benthamiana mesophyll cells elicited a large voltage-dependent inward chloride current across the tonoplast, showing that DTX33 and DTX35 each constitute a functional channel...
February 15, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28202455/in-the-interface-of-caesalpinioids-and-mimosoids-comparative-floral-development-elucidates-shared-characters-in-dimorphandra-mollis-and-pentaclethra-macroloba-leguminosae
#7
Thais C De Barros, Giseli D Pedersoli, Juliana V Paulino, Simone P Teixeira
PREMISE OF THE STUDY: Pentaclethra and Dimorphandra (Leguminosae) have long been considered a possible enigmatic link between caesalpinioids and mimosoids because they both have an imbricate calyx and heteromorphic androecium, floral features that are rare among mimosoids but common among caesalpinioids. This study compared the developing flowers of Dimorphandra mollis and Pentaclethra macroloba to determine whether the shared floral conditions also have the same ontogenetic origin. METHODS: Buds of different sizes and flowers were processed for surface (scanning electron microscopy) and histological (light microscopy) examination...
February 15, 2017: American Journal of Botany
https://www.readbyqxmd.com/read/28202454/pollinator-mediated-interactions-in-experimental-arrays-vary-with-neighbor-identity
#8
Melissa K Ha, Christopher T Ivey
PREMISE OF THE STUDY: Local ecological conditions influence the impact of species interactions on evolution and community structure. We investigated whether pollinator-mediated interactions between coflowering plants vary with plant density, coflowering neighbor identity, and flowering season. METHODS: We conducted a field experiment in which flowering time and floral neighborhood were manipulated in a factorial design. Early- and late-flowering Clarkia unguiculata plants were placed into arrays with C...
February 15, 2017: American Journal of Botany
https://www.readbyqxmd.com/read/28202451/losing-one-s-touch-evolution-of-the-touch-sensitive-stigma-in-the-mimulus-guttatus-species-complex
#9
Jannice Friedman, Katherine S Hart, Meghan C den Bakker
PREMISE OF THE STUDY: The stigmas of several species are touch sensitive and respond to pressure by closing. Previous research suggests that stigma closure could prevent self pollination within a flower during a pollinator's visit or enhance male function by increasing pollen export. Both factors could be favored in outcrossers, and neither would be beneficial in selfers. METHODS: We investigated variation in stigma-closing and the duration of closure in annual and perennial populations of the variable species Mimulus guttatus and whether four closely related selfing species (M...
February 15, 2017: American Journal of Botany
https://www.readbyqxmd.com/read/28199728/altered-mir-155-expression-in-allergic-asthmatic-airways
#10
Carina Malmhäll, Kristina Johansson, Carla Winkler, Sahar Alawieh, Linda Ekerljung, Madeleine Rådinger
MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at posttranscriptional level. We and others have previously identified miRNAs with pathological roles in animal models of asthma. To date, few studies have investigated miRNA expression in human asthmatics and miRNA expression in specific cellular subtypes in asthmatics is largely unexplored. Therefore, the aim of this study was to assess miR-146a and miR-155 expression in airways- and blood-derived samples, two miRNA candidates which have previously been described to play important roles in inflammatory processes...
February 15, 2017: Scandinavian Journal of Immunology
https://www.readbyqxmd.com/read/28198892/stimulated-raman-scattering-microscopy-and-spectroscopy-with-a-rapid-scanning-optical-delay-line
#11
Ruoyu He, Zhiping Liu, Yongkui Xu, Wei Huang, Hong Ma, Minbiao Ji
Stimulated Raman scattering (SRS) microscopy that is capable of both high-speed imaging and rapid spectroscopy will be advantageous for detailed chemical analysis of heterogeneous biological specimens. We have developed a system based on spectral focusing SRS technology with the integration of a rapid scanning optical delay line, which allows continuous tuning of SRS spectra by scanning a galvo mirror. We demonstrate SRS spectral measurements of dimethyl sulfoxide solution at low concentrations and multi-color imaging of rice pollens and HeLa cells with line-by-line delay tuning to reduce motion artifacts, as well as fast acquisition of SRS spectra at specific regions of interest...
February 15, 2017: Optics Letters
https://www.readbyqxmd.com/read/28196255/effect-of-2-years-of-treatment-with-sublingual-grass-pollen-immunotherapy-on-nasal-response-to-allergen-challenge-at-3-years-among-patients-with-moderate-to-severe-seasonal-allergic-rhinitis-the-grass-randomized-clinical-trial
#12
Guy W Scadding, Moises A Calderon, Mohamed H Shamji, Aarif O Eifan, Martin Penagos, Florentina Dumitru, Michelle L Sever, Henry T Bahnson, Kaitie Lawson, Kristina M Harris, Audrey G Plough, Joy Laurienzo Panza, Tielin Qin, Noha Lim, Nadia K Tchao, Alkis Togias, Stephen R Durham
Importance: Sublingual immunotherapy and subcutaneous immunotherapy are effective in seasonal allergic rhinitis. Three years of continuous treatment with subcutaneous immunotherapy and sublingual immunotherapy has been shown to improve symptoms for at least 2 years following discontinuation of treatment. Objective: To assess whether 2 years of treatment with grass pollen sublingual immunotherapy, compared with placebo, provides improved nasal response to allergen challenge at 3-year follow-up...
February 14, 2017: JAMA: the Journal of the American Medical Association
https://www.readbyqxmd.com/read/28195419/migratory-flight-behaviour-of-the-pollen-beetle-meligethes-aeneus
#13
Alice L Mauchline, Sam M Cook, Wilf Powell, Jason W Chapman, Juliet L Osborne
BACKGROUND: The field ecology of the pollen beetle Meligethes aeneus and its damaging effects on oilseed rape crops are well understood. However, the flight behaviour of M. aeneus, in particular the drivers for migratory movements across the landscape, is not well studied. We combined three established methodologies; suction traps, vertical-looking radar and high-altitude aerial netting to demonstrate that M. aeneus fly at a range of altitudes at different points during its active season...
February 13, 2017: Pest Management Science
https://www.readbyqxmd.com/read/28193870/disruption-of-quercetin-metabolism-by-fungicide-affects-energy-production-in-honey-bees-apis-mellifera
#14
Wenfu Mao, Mary A Schuler, May R Berenbaum
Cytochrome P450 monooxygenases (P450) in the honey bee, Apis mellifera, detoxify phytochemicals in honey and pollen. The flavonol quercetin is found ubiquitously and abundantly in pollen and frequently at lower concentrations in honey. Worker jelly consumed during the first 3 d of larval development typically contains flavonols at very low levels, however. RNA-Seq analysis of gene expression in neonates reared for three days on diets with and without quercetin revealed that, in addition to up-regulating multiple detoxifying P450 genes, quercetin is a negative transcriptional regulator of mitochondrion-related nuclear genes and genes encoding subunits of complexes I, III, IV, and V in the oxidative phosphorylation pathway...
February 13, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28191711/bioinformatic-screening-and-detection-of-allergen-cross-reactive-ige-binding-epitopes
#15
Scott McClain
Protein allergens can be related by cross-reactivity. Allergens that share relevant sequence can cross-react, those lacking similarity in their IgE antibody-binding epitopes do not cross-react. Cross-reactivity is based on shared epitopes which is due to shared sequence and higher level structure (charge and shape). Epitopes can be important in predicting cross-reactivity potential and may provide the potential to establish criteria that identify homology among allergens. Selected allergen's IgE binding epitope sequences were used to determine how the FASTA algorithm could be used to identify a threshold of significance...
February 13, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28188719/-systemic-reaction-after-performing-a-food-prick-to-prick-test-a-case-report
#16
Karen Estefanía Hernández-Moreno, Libia Susana Diez
BACKGROUND: Skin prick test is the most widely used test for the diagnosis of IgE-mediated conditions. Commercial extracts are used for its performance, but in the case of fruits and vegetables it is preferable using fresh food. Although both tests possess a good safety profile, hypersensitivity reactions have been recorded. CLINICAL CASE: Forty-seven-year old woman with a history of persistent allergic rhinitis, sensitized to the pollen of grasses, olive and salsola; she was referred to an allergology department due to anaphylaxis triggered by the consumption of avocado, cantaloupe, carrots and watermelon...
January 2017: Revista Alergia Mexico: Organo Oficial de la Sociedad Mexicana de Alergia e Inmunología, A.C
https://www.readbyqxmd.com/read/28188708/-skin-reactivity-frequency-to-aeroallergens-in-patients-with-clinical-symptoms-of-allergic-disease
#17
Isabel Cristina Rojas-Méndez, Oswaldo Arana-Muñoz, Aída Inés López-García, Daniela Rivero-Yeverino, Chrystopherson Gengyny Caballero-López, Sergio Papaqui-Tapia, Heriberto Camero-Martínez, Elizabeth Vázquez-Rojas
BACKGROUND: Allergic diseases diagnosis must be based on adequate allergological anamnesis and an immunological sensitization test; the most sensitive and specific is the skin prick test. OBJECTIVE: To determine the frequency of skin reactivity to aeroallergens, by age groups, in patients of the Department of Allergy and Clinical Immunology of the Hospital Universitario de Puebla, in Mexico. METHODS: A cross-sectional study was conducted that included patients aged 2 to 64 years with symptoms suggestive of allergic disease, in which skin prick tests with aeroallergens were performed; the diagnostic criteria were those of international guidelines...
January 2017: Revista Alergia Mexico: Organo Oficial de la Sociedad Mexicana de Alergia e Inmunología, A.C
https://www.readbyqxmd.com/read/28187589/palaearctic-i-protosmia-i-bees-of-the-subgenus-i-chelostomopsis%C3%A2-i-megachilidae-osmiini-biology-taxonomy-and-key-to-species
#18
Andreas Müller
Chelostomopsis represents a subgenus of the osmiine bee genus Protosmia (Megachilidae) containing three Palaearctic and one Nearctic species. Analysis of female pollen loads and literature data indicate that all Chelostomopsis species are broad pollen generalists exploiting the flowers of numerous plant families, such as Fabaceae, Asteraceae, Lamiaceae, Cistaceae and Brassicaceae. Preexisting linear cavities in dead wood or pine cones serve as nesting sites and pure resin is used to construct cell partitions and nest plug...
February 2, 2017: Zootaxa
https://www.readbyqxmd.com/read/28187378/development-of-an-analytical-method-for-detecting-nitrofurans-in-bee-pollen-by-liquid-chromatography-electrospray-ionization-tandem-mass-spectrometry
#19
Mi Sun Park, Kyung Tae Kim, Jong Seong Kang
Bee pollen collected by honeybees, which is in powdered form, is a good nutritional supplement. Nitrofuran antibiotics are assumed not to be present in bee pollen, which is important as the level of antibiotics in bee pollen is strongly regulated in many countries. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to detect nitrofurans in honey has been developed, but this method is not suitable for bee pollen because of it being in powdered form. During preparation of bee pollen samples, the dispersal of powder particles in an aqueous solution often makes them susceptible to forming an emulsion with solvent components such as hexane and ethyl acetate...
January 9, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28187145/a-new-sythetic-hybrid-a1d5-between-gossypium-herbaceum-and-g-raimondii-and-its-morphological-cytogenetic-molecular-characterization
#20
Yuxiang Wu, Di Chen, Shuijin Zhu, Lufei Zhang, Lingjiao Li
The diploid species G. herbaceum (A1) and G. raimondii (D5) are the progenitors of allotetraploid cotton, respectively. However, hybrids between G. herbaceum and G. raimondii haven't been reported. In the present study, hybridization between G. herbaceum and G. raimondii was explored. Morphological, cytogenetic and molecular analyses were used to assess the hybridity. The interspecific hybrid plants were successfully obtained. Most of the morphological characteristics of the hybrids were intermediate between G...
2017: PloS One
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