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https://www.readbyqxmd.com/read/28934010/down-regulation-of-klf5-in-cancer-associated-fibroblasts-inhibit-gastric-cancer-cells-progression-by-ccl5-ccr5-axis
#1
Tingsong Yang, Min Chen, Yingying Sun, Xiaobing Zhang, Zhou Zhang, Bin Xu, Jie Hua, Zhigang He, Zhenshun Song
It was well known that cancer-associated fibroblasts (CAFs) were an essential factor in tumor progression. However, the actual mechanism of stromal fibroblasts activation and tumor promoting effects remain unclear. Here, we showed that KLF5 expression was more frequently observed in gastric cancer-associated fibroblasts compared with normal mucosal fibroblasts. Moreover, KLF5 expression in tumor stroma was closely associated with clinicopathological features such as tumor size, invasion depth, cell grade and lymph node metastasis, as well as poor prognosis in patients with gastric cancer...
September 21, 2017: Cancer Biology & Therapy
https://www.readbyqxmd.com/read/28933630/going-native-direct-high-throughput-screening-of-secreted-full-length-igg-antibodies-against-cell-membrane-proteins
#2
Yongliang Fang, Thach H Chu, Margaret E Ackerman, Karl E Griswold
Gel microdroplet - fluorescence activated cell sorting (GMD-FACS) is an innovative high throughput screening platform for recombinant protein libraries, and we show here that GMD-FACS can overcome many of the limitations associated with conventional screening methods for antibody libraries. For example, phage and cell surface display benefit from exceptionally high throughput, but generally require high quality, soluble antigen target and necessitate the use of anchored antibody fragments. In contrast, the GMD-FACS assay can screen for soluble, secreted, full-length IgGs at rates of several thousand clones per second, and the technique enables direct screening against membrane protein targets in their native cellular context...
September 21, 2017: MAbs
https://www.readbyqxmd.com/read/28926402/reversing-hiv-latency-via-sphingosine-1-phosphate-receptor-1-signaling
#3
Charline Duquenne, Sandrine Gimenez, Adeline Guigues, Benjamin Viala, Caroline Boulouis, Clément Mettling, Damien Maurel, Noëlie Campos, Etienne Doumazane, Laetitia Comps-Agrar, Jamal Tazi, Laurent Prézeau, Pierre Corbeau, Vincent François
OBJECTIVE: In this study, we looked for a new family of latency reversing agents. DESIGN: We searched for G protein-coupled receptors (GPCR) coexpressed with CCR5 in primary CD4+ T cells that activate infected cells and boost HIV production. METHODS: GPCR coexpression was unveiled by RT-PCR. We used FRET to analyze the dimerization with CCR5 of the expressed GPCR. Viral entry was measured by flow cytometry, reverse transcription by quantitative PCR, NFkB translocation by immunofluorescence, LTR activation using a gene reporter assay, and viral production by p24 quantification...
September 18, 2017: AIDS
https://www.readbyqxmd.com/read/28919783/toll-like-receptors-7-and-8-expression-correlates-with-the-expression-of-immune-biomarkers-and-positively-predicts-the-clinical-outcome-of-patients-with-melanoma
#4
Moucheng Zhang, Zhilong Yan, Junjiang Wang, Xueqing Yao
BACKGROUND: Toll-like receptors (TLRs) play a critical role in cancer, yet the clinical relevance of TLR7/8 expression in melanoma remains unclear. This study aimed to evaluate the prognostic value of TLR7/8 mRNA levels in melanoma and their correlation with immune biomarkers relevant to disease progression. METHODS: Normalized gene expression and corresponding clinical data of patients with skin cutaneous melanoma were obtained from two public databases: the Cancer Genome Atlas and GSE19234...
2017: OncoTargets and Therapy
https://www.readbyqxmd.com/read/28918646/depletion-of-gut-resident-ccr5-cells-for-hiv-cure-strategies
#5
David Patrick Merriam, Connie Chen, Gema Mendez-Lagares, Kenneth Rogers, Anthony Michaels, Jiangli Yan, Paul Casaz, Keith Reimann, Francois Villenger, Dennis J Hartigan-O'Connor
The HIV reservoir forming at the earliest stages of infection is likely composed of CCR5+ cells, because these cells are the targets of transmissible virus. Restriction of the CCR5+ reservoir, particularly in gut, may be needed for subsequent cure attempts. Strategies for killing or depleting CCR5+ cells have been described, but none have been tested in vivo in non-human primates and the extent of achievable depletion from tissues is not known. Here we investigate the efficacy of two novel cytotoxic treatments for targeting and eliminating CCR5+ cells in young rhesus macaques...
September 17, 2017: AIDS Research and Human Retroviruses
https://www.readbyqxmd.com/read/28910354/differential-impact-of-the-dual-ccr2-ccr5-inhibitor-cenicriviroc-on-migration-of-monocyte-and-lymphocyte-subsets-in-acute-liver-injury
#6
Tobias Puengel, Oliver Krenkel, Marlene Kohlhepp, Eric Lefebvre, Tom Luedde, Christian Trautwein, Frank Tacke
A hallmark of acute hepatic injury is the recruitment of neutrophils, monocytes and lymphocytes, including natural killer (NK) or T cells, towards areas of inflammation. The recruitment of leukocytes from their reservoirs bone marrow or spleen into the liver is directed by chemokines such as CCL2 (for monocytes) and CCL5 (for lymphocytes). We herein elucidated the impact of chemokine receptor inhibition by the dual CCR2 and CCR5 inhibitor cenicriviroc (CVC) on the composition of myeloid and lymphoid immune cell populations in acute liver injury...
2017: PloS One
https://www.readbyqxmd.com/read/28904745/genome-editing-of-the-hiv-co-receptors-ccr5-and-cxcr4-by-crispr-cas9-protects-cd4-t-cells-from-hiv-1-infection
#7
Zhepeng Liu, Shuliang Chen, Xu Jin, Qiankun Wang, Kongxiang Yang, Chenlin Li, Qiaoqiao Xiao, Panpan Hou, Shuai Liu, Shaoshuai Wu, Wei Hou, Yong Xiong, Chunyan Kong, Xixian Zhao, Li Wu, Chunmei Li, Guihong Sun, Deyin Guo
BACKGROUND: The main approach to treat HIV-1 infection is combination antiretroviral therapy (cART). Although cART is effective in reducing HIV-1 viral load and controlling disease progression, it has many side effects, and is expensive for HIV-1 infected patients who must remain on lifetime treatment. HIV-1 gene therapy has drawn much attention as studies of genome editing tools have progressed. For example, zinc finger nucleases (ZFN), transcription activator like effector nucleases (TALEN) and clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 have been utilized to successfully disrupt the HIV-1 co-receptors CCR5 or CXCR4, thereby restricting HIV-1 infection...
2017: Cell & Bioscience
https://www.readbyqxmd.com/read/28904130/ccr5-dependent-homing-of-t-regulatory-cells-to-the-tumor-microenvironment-contributes-to-skin-squamous-cell-carcinoma-development
#8
Carine Ervolino de Oliveira, Thais Helena Gasparoto, Claudia Ramos Pinheiro, Nádia Ghinelli Amôr, Maria Renata Sales Nogueira, Ramon Kaneno, Gustavo Pompermaier Garlet, Vanessa Soares Lara, João Santana da Silva, Karen Angélica Cavassani, Ana Paula Campanelli
Squamous cell carcinoma (SCC) is one of the most common human cancers worldwide. Recent studies show that regulatory T cells (Tregs) have a critical role in the modulation of an anti- tumor immune response, and consequently the SCC development. Since the accumulation of Tregs at the tumor site is, in part, due to selective recruitment through CCR5 and CCR5-associated chemokines, we investigated the role of CCR5 in the SCC development. Our findings showed that CCR5-deficient mice (CCR5KO) were efficient in controlling papilloma's incidence when compared with wild-type mice...
September 13, 2017: Molecular Cancer Therapeutics
https://www.readbyqxmd.com/read/28903065/inflammation-polymorphisms-and-prostate-cancer-risk-in-jamaican-men-role-of-obesity-body-size
#9
Bhawna Dubey, Maria Jackson, Charnita Zeigler-Johnson, Karthik Devarajan, Rafael E Flores-Obando, Norma McFarlane-Anderson, Marshall Tulloch-Reid, William Aiken, Kevin Kimbro, Dominique Jones, LaCreis R Kidd, Camille Ragin
African ancestry and obesity are associated with higher risk of prostate cancer (PC). In a pilot study, we explored interactions between obesity (as measured by waist to hip ratio (WHR)) and inflammatory SNPs in relation to PC risk among Jamaican men. This study evaluated 87 chemokine and cytokine associated SNPs in obese and normal weight cases (N=109) and controls (N=102) using a stepwise penalized logistic regression approach in multivariable analyses. Upon stratification by WHR (normal weight (WHR<0...
September 10, 2017: Gene
https://www.readbyqxmd.com/read/28902921/correction-cd4-is-expressed-on-a-heterogeneous-subset-of-hematopoietic-progenitors-which-persistently-harbor-cxcr4-and-ccr5-tropic-hiv-proviral-genomes-in-vivo
#10
(no author information available yet)
[This corrects the article DOI: 10.1371/journal.ppat.1006509.].
September 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28899878/endothelial-cells-promote-triple-negative-breast-cancer-cell-metastasis-via-pai-1-and-ccl5-signaling
#11
Wenwen Zhang, Jing Xu, Hehui Fang, Lin Tang, Weiwei Chen, Qian Sun, Qun Zhang, Fang Yang, Zijia Sun, Lulu Cao, Yucai Wang, Xiaoxiang Guan
Endothelial cells (ECs) in the tumor microenvironment have been reported to play a more active role in solid tumor growth and metastatic dissemination than simply providing the physical structure to form conduits for blood flow; however, the involvement of ECs in the process of triple-negative breast cancer (TNBC) metastasis has not been addressed. Here, we demonstrate that ECs-when mixed with TNBC cells-could increase TNBC cell metastatic potency. After treatment with TGF-β to induce endothelial-mesenchymal transition (EMT), TNBC cells could produce plasminogen activator inhibitor-1 (PAI-1) and stimulate the expression and secretion of the chemokine, CCL5, from ECs, which then acts in a paracrine fashion on TNBC cells to enhance their migration, invasion, and metastasis...
September 12, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28894259/cd161-identifies-polyfunctional-th1-th17-cells-in-the-genital-mucosa-that-are-depleted-in-hiv-infected-female-sex-workers-from-nairobi-kenya
#12
Geneviève Boily-Larouche, Kenneth Omollo, Julianna Cheruiyot, Jane Njoki, Makobu Kimani, Joshua Kimani, Julius Oyugi, Julie Lajoie, Keith R Fowke
CD161 identifies a subset of circulating Th17 cells that are depleted in the blood and gut of HIV-infected individuals. In the female reproductive tract (FRT), the pattern of CD161 expression on CD4(+) cells remains unknown. Here, we characterized CD161 expression in the FRT of Kenyan female sex workers (FSW). Compared to the blood, CD161(+)CD4(+) T cells were enriched in the FRT of uninfected FSWs. These cells were depleted in FRT of HIV-infected FSWs. Cervical CD161(+) cells harboured an activated phenotype (CD69, CD95, HLA-DR) with elevated expression of tissue-homing markers (CCR6, β7 integrin) and HIV co-receptor (CCR5)...
September 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28894041/analysis-of-the-relationship-between-single-nucleotide-polymorphism-of-the-cd209-il-10-il-28-and-ccr5-d32-genes-with-the-human-predisposition-to-developing-tick-borne-encephalitis
#13
Piotr Czupryna, Miłosz Parczewski, Sambor Grygorczuk, Sławomir Pancewicz, Joanna Zajkowska, Justyna Dunaj, Maciej Kondrusik, Katarzyna Krawczuk, Anna Moniuszko-Malinowska
<b>Introduction: </b>It is known that in the pathogenesis of tick-borne encephalitis (TBE) various molecules play a significant role. The most prominent factors include IL-10, IL-28B, CD-209 and CCR5. It is reasonable to search for genetic predispositions to the development of various clinical forms of TBE related to the genetic variation of IL-10, IL-28B, CD-209 and CCR5. In this study we aimed to search for the relationship between single nucleotide polymorphism in the promoter region of the CD209, IL-10, IL-28 and 32 base pair deletion in CCR5 coding region (Δ 32) with the human predisposition to development of various clinical presentations of TBE...
January 4, 2017: Postȩpy Higieny i Medycyny Doświadczalnej
https://www.readbyqxmd.com/read/28894001/intestinal-type-1-regulatory-t-cells-migrate-to-periphery-to-suppress-diabetogenic-t-cells-and-prevent-diabetes-development
#14
Hua Yu, Nicola Gagliani, Harumichi Ishigame, Samuel Huber, Shu Zhu, Enric Esplugues, Kevan C Herold, Li Wen, Richard A Flavell
Growing insight into the pathogenesis of autoimmune diseases and numerous studies in preclinical models highlights the potential of regulatory T cells to restore tolerance. By using non-obese diabetic (NOD) BDC2.5 TCR-transgenic (Tg), and IL-10 and Foxp3 double-reporter mice, we demonstrate that alteration of gut microbiota during cohousing experiments or treatment with anti-CD3 mAb significantly increase intestinal IL-10-producing type 1 regulatory T (Tr1) cells and decrease diabetes incidence. These intestinal antigen-specific Tr1 cells have the ability to migrate to the periphery via a variety of chemokine receptors such as CCR4, CCR5, and CCR7 and to suppress proliferation of Th1 cells in the pancreas...
September 11, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28881626/relevance-of-ccl3-ccr5-axis-in-oral-carcinogenesis
#15
Janine Mayra da Silva, Tálita Pollyanna Moreira Dos Santos, Lays Martin Sobral, Celso Martins Queiroz-Junior, Milene Alvarenga Rachid, Amanda E I Proudfoot, Gustavo Pompermaier Garlet, Aline Carvalho Batista, Mauro Martins Teixeira, Andréia Machado Leopoldino, Remo Castro Russo, Tarcília Aparecida Silva
The chemokine CCL3 is a chemotactic cytokine crucial for inflammatory cell recruitment in homeostatic and pathological conditions. CCL3 might stimulate cancer progression by promoting leukocyte accumulation, angiogenesis and tumour growth. The expression of CCL3 and its receptors CCR1 and CCR5 was demonstrated in oral squamous cell carcinoma (OSCC), but their role was not defined. Here, the functions of CCL3 were assessed using a model of chemically induced tongue carcinogenesis with 4-nitroquinoline-1-oxide (4NQO)...
August 1, 2017: Oncotarget
https://www.readbyqxmd.com/read/28868628/ovarian-cancer-stem-cells-promote-tumour-immune-privilege-and-invasion-via-ccl5-and-regulatory-t-cells
#16
Yanan You, Yiying Li, Ming Li, Lei Mi, Mengyao Wu, Ying Qu, Yan Yuan, Tong Chen, Hua Jiang
Emerging evidence indicates a link between the increased proportion of regulatory T cells (Tregs) and reduced survival in patients who have been diagnosed with cancer. Cancer stem cells (CSCs) have been indicated to play a vital role in tumour initiation, drug resistance and recurrence. However, the relationship between Tregs and CSCs remains largely unknown. Here, we sorted out ovarian cancer stem-like side population (SP) cells and CD133(+) cells to investigate the influence of ovarian CSCs on Tregs. Among the various immune-related molecules that we assessed, C-C motif chemokine ligand 5 (CCL5) was the most elevated in ovarian CSCs relative to that in the non-CSCs...
September 4, 2017: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/28866647/chemotaxis-of-v%C3%AE-2-t-cells-to-the-joints-contributes-to-the-pathogenesis-of-rheumatoid-arthritis
#17
Wen-Xiu Mo, Shan-Shan Yin, Hua Chen, Chen Zhou, Jia-Xin Zhou, Li-Dan Zhao, Yun-Yun Fei, Hua-Xia Yang, Jing-Bo Guo, Yu-Jia Mao, Lin-Fang Huang, Wen-Jie Zheng, Wen Zhang, Jian-Min Zhang, Wei He, Xuan Zhang
OBJECTIVES: To explore the role of Vδ2 T cells in the pathogenesis of rheumatoid arthritis (RA). METHODS: Sixty-eight patients with RA, 21 patients with osteoarthritis and 21 healthy controls were enrolled in the study. All patients with RA fulfilled the 2010 American College of Rheumatology/European League Against Rheumatism criteria for RA. Peripheral Vδ2T population, chemokine receptor expression and proinflammatory cytokine secretion were quantified by flow cytometry...
September 2, 2017: Annals of the Rheumatic Diseases
https://www.readbyqxmd.com/read/28862353/the-effect-of-ccr5%C3%AE-32-on-the-risk-of-grade-3-4-acute-graft-versus-host-disease-after-allogeneic-hematopoietic-stem-cell-transplantation-a-systematic-review-and-meta-analysis
#18
REVIEW
Jingwen Niu, Ting Pan, Bin Zhang, Chen Hu
OBJECTIVE: Many studies have showed that CCR5 was involved in the pathological process of acute graft-versus-host disease (aGVHD). However, the relationship between CCR5Δ32, a frameshift mutation, and grade 3-4 acute GVHD risk has not been well established. We performed a meta-analysis to explore the effects of CCR5 gene polymorphisms on grade 3-4 aGVHD. METHODS: PubMed, Embase, Web of Science and Cochrane Library were searched to collect relevant articles. Studies about association between CCR5 genotype in recipients or donors of allo-HSCT and aGVHD risk were included...
September 1, 2017: Clinical Transplantation
https://www.readbyqxmd.com/read/28857825/preferential-susceptibility-of-th9-and-th2-cd4-t-cells-to-x4-tropic-hiv-1-infection
#19
Nina Orlova-Fink, Fatema Z Chowdhury, Sun Xiaoming, Sean Harrington, Eric S Rosenberg, Xu G Yu, Mathias Lichterfeld
OBJECTIVE: The functional polarization of CD4 T cells determines their antimicrobial effector profile, but may also impact the susceptibility to infection with HIV-1. Here, we analyzed the susceptibility of CD4 T cells with different functional polarization to infection with X4- and R5-tropic HIV-1. METHODS: CD4 T cells with a Th1, Th2, Th17 and Th9 polarization were subjected to in vitro infection assays with X4, R5 or VSV-G-pseudotyped HIV-1. In addition, we sorted differentially-polarized CD4 T cell subsets from individuals treated with antiretroviral therapy and analyzed the tropism of viral env sequences...
August 28, 2017: AIDS
https://www.readbyqxmd.com/read/28852441/expression-of-pro-inflammatory-genes-in-lesions-spleens-and-blood-neutrophils-after-burn-injuries-in-mice-treated-with-silver-sulfodiazine
#20
Soheyla Akhzari, Hossein Rezvan, Seyed Masoud Zolhavarieh
OBJECTIVES: It is now supposed that cytokines released during the burn injuries have a great impact on the immunological and pathological responses after the burn. The main objective of this study was to investigate the expression of some pro-inflammatory genes in the wound, spleen and blood neutrophils during the healing process of burn wounds in a murine model. MATERIALS AND METHODS: The expression of ten pro-inflammatory genes were examined in wounds, spleens and blood neutrophils of mice with burn injuries treated with either silver sulfodiazine or phosphate-buffered saline (PBS) using RT-PCR at the end of the first and second weeks after injuries...
July 2017: Iranian Journal of Basic Medical Sciences
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