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https://www.readbyqxmd.com/read/29223009/influence-of-spermatozoal-lipidomic-profile-on-the-cryoresistance-of-frozen-spermatozoa-from-stallions
#1
T Cabrera, C Ramires-Neto, K R A Belaz, C P Freitas-Dell'aqua, D Zampieri, A Tata, M N Eberlin, M A Alvarenga, F F Souza
The membrane of spermatozoa, which contributes to cellular cryoresistance, contains numerous lipids with a composition that directly affects membrane fluidity and the fertilization process. In light of variations in the degree of sensitivity in equine seminal freezing, this study aimed to correlate equine semen lipids with post-thawing characteristics of spermatozoa. We used ejaculates from 34 stallions, which were evaluated (total motility ≥ 60%), frozen and thawed and reevaluated for motility of spermatozoa, membrane integrity and lipid peroxidation...
November 26, 2017: Theriogenology
https://www.readbyqxmd.com/read/29219746/development-of-two-surgical-approaches-to-the-pituitary-gland-in-the-horse
#2
James L Carmalt, Brian A Scansen
BACKGROUND: Current treatment of equine PPID requires daily oral medication, which is expensive and difficult from a management perspective. A minimally invasive surgical palliation of this chronic condition is appealing because it is envisioned to be a single treatment which, while not curative, will alleviate the clinical signs of disease dramatically thereby improving the welfare of the horse. OBJECTIVE: To develop a surgical approach to the equine pituitary gland, for subsequent treatment of pituitary pars intermedia dysfunction...
December 8, 2017: Veterinary Quarterly
https://www.readbyqxmd.com/read/29216299/recombinant-vaccinia-virus-coded-interferon-inhibitor-b18r-expression-refolding-and-a-use-in-a-mammalian-expression-system-with-a-rna-vector
#3
Yuriy G Kim, Aliya Zh Baltabekova, Erzhan E Zhiyenbay, Altynai S Aksambayeva, Zhadyra S Shagyrova, Rinat Khannanov, Erlan M Ramanculov, Alexandr V Shustov
B18R protein of Vaccinia virus binds to type I interferons and inhibits activation of interferon-mediated signal transduction. Cells which have unimpaired interferon signaling such as primary cell cultures or some industrially important cell lines are capable of development of an antiviral state. An establishment of the antiviral state limits replication of RNA-viruses and can suppress replication of RNA vectors. The interferon inhibitor B18R effectively prevents the establishment of the antiviral state. For this reason, B18R has become a ubiquitous component of protocols for epigenetic reprogramming which use transfections of RNA replicons or mRNA...
2017: PloS One
https://www.readbyqxmd.com/read/29216266/identification-and-genetic-characterization-of-hepacivirus-and-pegivirus-in-commercial-equine-serum-products-in-china
#4
Gang Lu, Ji Huang, Qiliang Yang, Haibin Xu, Peixin Wu, Cheng Fu, Shoujun Li
Equine hepacivirus (EqHV), equine pegivirus (EPgV) and Theiler's disease-associated virus (TDAV) are three novel equine viruses in the family Flaviviridae. EqHV and EPgV have been identified to circulate in the equine population worldwide, whereas TDAV has not been detected in equines since the first reported case. To date, no studies have focused on investigating EqHV, EPgV and TDAV in commercial equine sera or equine blood products in China. Considering the potential threat of EqHV, EPgV and TDAV to biosecurity and considering their possible influences on research results, equine sera for cell culture propagation and pregnant mare serum gonadotropin (PMSG) were purchased from different companies in China and investigated for EqHV, EPgV and TDAV in this study...
2017: PloS One
https://www.readbyqxmd.com/read/29209631/maxillary-sinus-floor-augmentation-using-an-equine-derived-graft-material-preliminary-results-in-17-patients
#5
F Rivara, M Negri, S Lumetti, L Parisi, A Toffoli, E Calciolari, E Manfredi, G M Macaluso
Objective: Sinus floor elevation with lateral approach is probably the most frequently performed reconstructive procedure to rehabilitate posterior maxilla when a bone deficiency is present. Different graft materials have been proposed and tested, often with high clinical performances and predictable results. Histological analysis is required when evaluating new materials. We investigated human biopsies retrieved after sinus floor elevation procedure by histomorphometric evaluation to test the performance of an equine-derived bone grafting material...
2017: BioMed Research International
https://www.readbyqxmd.com/read/29208690/aortic-hemodynamics-in-postmenopausal-women-following-cessation-of-hormone-therapy
#6
Ronée E Harvey, Maja C Johnson, Sushant M Ranadive, Michael J Joyner, Brian D Lahr, Virginia M Miller, Jill N Barnes
Central (aortic) blood pressure and aortic pulse wave characteristics are measures of cardiovascular health, predictive of cardiovascular mortality. Previous studies have compared aortic hemodynamics in women who do and do not take menopausal hormone therapy, but characteristics of these parameters following cessation of treatment have not been defined. Therefore, the purpose of this study was to define aortic pulse wave characteristics in postmenopausal women with and without a history of menopausal hormone therapy use...
December 2017: Physiological Reports
https://www.readbyqxmd.com/read/29208213/evaluation-of-ex-vivo-restoration-of-carious-equine-maxillary-cheek-teeth-infundibulae-following-debridement-with-dental-drills-and-hedstrom-files
#7
A Horbal, R J M Reardon, T Liuti, P M Dixon
An ex-vivo study was performed on vertically mounted equine maxillary cheek teeth that had caries of 30 infundibulae, using high and low speed contra-angle dental drills and Hedstrom files to debride infundibular food debris and carious dental tissues. This technique effectively debrided 27/30 infundibulae with a mean depth of 19mm (range 9.4-34.3mm) as assessed by computed tomographic (CT) imaging. The debrided infundibulae were treated with sodium hypochlorite, EDTA and a bonding agent, and then then filled in layers with endodontic restoration materials...
December 2017: Veterinary Journal
https://www.readbyqxmd.com/read/29208065/use-of-confocal-microscopy-to-evaluate-equine-zygote-development-after-sperm-injection-of-oocytes-matured-in-vivo-or-in-vitro
#8
Elena Ruggeri, Keith F DeLuca, Cesare Galli, Giovanna Lazzari, Jennifer G DeLuca, Joanne E Stokes, Elaine M Carnevale
Confocal microscopy was used to image stages of equine zygote development, at timed intervals, after intracytoplasmic sperm injection (ICSI) of oocytes that were matured in vivo or in vitro. After fixation for 4, 6, 8, 12, or 16 h after ICSI, zygotes were incubated with α/β tubulin antibodies and human anticentromere antibody (CREST/ACA), washed, incubated in secondary antibodies, conjugated to either Alexa 488 or Alexa 647, and incubated with 561-Phalloidin and Hoechst 33258. An Olympus IX81 spinning disk confocal microscope was used for imaging...
December 6, 2017: Microscopy and Microanalysis
https://www.readbyqxmd.com/read/29207516/equine-vaccines-how-when-and-why-report-of-the-vaccinology-session-french-equine-veterinarians-association-2016-reims
#9
Romain Paillot, Christel Marcillaud Pitel, Xavier D'Ablon, Stéphane Pronost
To date, vaccination is one of the most efficient methods of prevention against equine infectious diseases. The vaccinology session, which was organised during the annual meeting of the French Equine Veterinarians Association (AVEF) at Reims (France) in 2016, aimed to approach three subjects of importance for the equine industry. Vaccination against three major equine diseases were used as examples: equine influenza (equine influenza virus), rhinopneumonitis (equine herpes virus 1/4), and tetanus (Clostridium tetani neuro-toxin)...
December 4, 2017: Vaccines
https://www.readbyqxmd.com/read/29207466/evaluation-of-poly-lactic-co-glycolic-acid-alone-or-in-combination-with-hydroxyapatite-on-human-periosteal-cells-bone-differentiation-and-in-sinus-lift-treatment
#10
Gabriele Ceccarelli, Rossella Presta, Saturnino Marco Lupi, Nefele Giarratana, Nora Bloise, Laura Benedetti, Maria Gabriella Cusella De Angelis, Ruggero Rodriguez Y Baena
Most recent advances in tissue engineering in the fields of oral surgery and dentistry have aimed to restore hard and soft tissues. Further improvement of these therapies may involve more biological approaches and the use of dental tissue stem cells in combination with inorganic/organic scaffolds. In this study, we analyzed the osteoconductivity of two different inorganic scaffolds based on poly (lactic-co-glycolic) acid alone (PLGA-Fisiograft) or in combination with hydroxyapatite (PLGA/HA-Alos) in comparison with an organic material based on equine collagen (PARASORB Sombrero) both in vitro and in vivo...
December 2, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29206788/matrix-induced-autologous-chondrocyte-implantation-maci-using-a-cell-seeded-collagen-membrane-improves-cartilage-healing-in-the-equine-model
#11
Alan J Nixon, Holly D Sparks, Laila Begum, Sean McDonough, Michael S Scimeca, Nance Moran, Gloria L Matthews
BACKGROUND: Autologous chondrocyte implantation (ACI) using a collagen scaffold (matrix-induced ACI; MACI) is a next-generation approach to traditional ACI that provides the benefit of autologous cells and guided tissue regeneration using a biocompatible collagen scaffold. The MACI implant also has inherent advantages including surgical implantation via arthroscopy or miniarthrotomy, the elimination of periosteal harvest, and the use of tissue adhesive in lieu of sutures. This study evaluated the efficacy of the MACI implant in an equine full-thickness cartilage defect model at 1 year...
December 6, 2017: Journal of Bone and Joint Surgery. American Volume
https://www.readbyqxmd.com/read/29206130/the-contribution-of-oxidative-stress-and-inflamm-aging-in-human-and-equine-asthma
#12
Michela Bullone, Jean-Pierre Lavoie
Aging is associated with a dysregulation of the immune system, leading to a general pro-inflammatory state of the organism, a process that has been named inflamm-aging. Oxidative stress has an important role in aging and in the regulation of immune responses, probably playing a role in the development of age-related diseases. The respiratory system function physiologically declines with the advancement of age. In elderly asthmatic patients, this may contribute to disease expression. In this review, we will focus on age-related changes affecting the immune system and in respiratory structure and function that could contribute to asthma occurrence, and/or clinical presentation in the elderly...
December 5, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29204821/development-of-a-clinical-prediction-score-for-detection-of-suspected-cases-of-equine-grass-sickness-dysautonomia-in-france
#13
P K Randleff-Rasmussen, A Leblond, J Cappelle, J Bontemps, S Belluco, M R Popoff, C Marcillaud-Pitel, J Tapprest, P Tritz, I Desjardins
Equine grass sickness (EGS) (equine dysautonomia) is a neurodegenerative condition of grazing equines. Pre-mortem diagnosis of EGS is a challenge for practitioners as definitive diagnosis requires ileal/myenteric lymph node biopsies. This study aimed to develop a clinical score that could be used by practitioners to improve the detection of acute or subacute EGS cases in the field. Suspected EGS cases were declared by veterinary practitioners. A case was classified as confirmed positive if ileal or rectal biopsy samples showed neuronal degeneration typical of EGS...
December 4, 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/29203939/a-retrospective-study-of-owner-requested-testing-as-surveillance-for-equine-infectious-anemia-in-canada-2009-2012
#14
Sara N Higgins, Krista J Howden, Carolyn R James, Tasha Epp, Katharina L Lohmann
This retrospective study was undertaken to estimate i) the surveillance coverage for equine infectious anemia (EIA) based on owner-requested testing, and ii) the incidence of case detection from this surveillance activity to inform a review of Canada's national disease control strategy. Based on sample submissions by accredited veterinarians to laboratories CFIA-approved for EIA testing between 2009 and 2012, the estimated national surveillance coverage was 14% for all years, and 72 cases of EIA were detected...
December 2017: Canadian Veterinary Journal. la Revue Vétérinaire Canadienne
https://www.readbyqxmd.com/read/29203401/skeletal-muscle-from-aged-american-quarter-horses-shows-impairments-in-mitochondrial-biogenesis-and-expression-of-autophagy-markers
#15
Chengcheng Li, Sarah H White, Lori K Warren, Stephanie E Wohlgemuth
Aging is associated with decreased mitochondrial content and function in skeletal muscle, possibly due to compromised biogenesis and autophagic removal of dysfunctional mitochondria. The aim of this study was to compare markers of mitochondrial content and biogenesis and of autophagy between skeletal muscle from young and aged American Quarter Horses. Citrate synthase protein and mtDNA copy number were decreased in triceps brachii (TB) muscle (P<0.05) from aged horses, suggesting an age-related decline in mitochondrial content...
December 1, 2017: Experimental Gerontology
https://www.readbyqxmd.com/read/29202675/acute-hepatic-steatosis-a-helpful-diagnostic-feature-in-metallic-phosphide-poisoned-horses
#16
Jonathan H Fox, Brian F Porter, Leslie Easterwood, Justin R V Hildenbrand, Pierre Hélie, James Smylie, Donal O'Toole
Metal phosphides, particularly zinc and aluminum phosphide, occasionally poison horses and other equids following their use as rodenticides and insecticides. Grain-based aluminum phosphide baits are used to control rodents such as prairie dogs. The clinical course in intoxicated horses is short (<24-48 h), and animals may be found dead. Hepatic lesions caused by phosphine poisoning are not well described. Laboratory confirmation depends on detecting phosphine gas in gastric contents. Eight horses and a mule were exposed to zinc phosphide used to control prairie dogs on a Wyoming ranch...
December 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/29199537/equine-renal-hemangiosarcoma-clinical-presentation-pathologic-features-and-pstat3-expression
#17
Katherine Hughes, Victoria H L Scott, Maximilian Blanck, Timothy P Barnett, Jannie Spanner Kristiansen, Alastair K Foote
Hemangiosarcoma is an uncommon tumor in horses. We characterized 3 cases of equine renal hemangiosarcoma, focusing on clinical and pathologic features, and describe occurrence of the epithelioid variant of hemangiosarcoma in one of these cases. Nuclear expression of phosphorylated STAT3 (pSTAT3) was assessed to analyze potential inappropriate STAT3 activation as a component of tumor pathogenesis. Clinical signs in the 3 horses included insidious weight loss, followed in one case by serosanguineous nasal discharge and terminal epistaxis, and nonspecific signs of abdominal pain...
December 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/29199039/the-coronavirus-nucleocapsid-protein-is-adp-ribosylated
#18
Matthew E Grunewald, Anthony R Fehr, Jeremiah Athmer, Stanley Perlman
ADP-ribosylation is a common post-translational modification, although how it modulates RNA virus infection is not well understood. While screening for ADP-ribosylated proteins during coronavirus (CoV) infection, we detected a ~55kDa ADP-ribosylated protein in mouse hepatitis virus (MHV)-infected cells and in virions, which we identified as the viral nucleocapsid (N) protein. The N proteins of porcine epidemic diarrhea virus (PEDV), severe acute respiratory syndrome (SARS)-CoV and Middle East respiratory syndrome (MERS)-CoV were also ADP-ribosylated...
November 30, 2017: Virology
https://www.readbyqxmd.com/read/29198956/effects-of-staining-method-and-clinician-experience-on-the-evaluation-of-stallion-sperm-morphology
#19
Ruby Yoana Murcia-Robayo, Eleonore Jouanisson, Guy Beauchamp, Mouhamadou Diaw
Evaluation of sperm morphology is part of the assessment of fertility in human and animal reproduction. Analyses can be performed using different techniques, including the use of staining methods In our prospective study, the morphology of equine sperm was evaluated using 3 staining methods Diff-Quik, eosin-nigrosin, and SpermBlue, the latter being a relatively new stain commonly used for human sperm. Our hypotheses were that (1) SpermBlue allows easier assessment of horse sperm morphology and facilitates better identification of sperm abnormalities, and (2) sperm morphology classification differs depending on the evaluator's experience...
November 29, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/29198841/-antimicrobial-susceptibility-in-isolates-of-streptococcus-equi-subsp-equi-from-buenos-aires-province-argentina
#20
Carla P Bustos, María J Marfil, Natalia S Lanza, Nora Guida
Streptococcus equi subsp. equi is the etiologic agent of strangles, an infectious disease affecting the upper respiratory tract and head and neck lymph nodes of equines. Routine antimicrobial therapy includes penicillin (PEN) as antibiotic of first choice. Streptococci are usually susceptible to PEN and only a few antimicrobial studies had been performed. The aim of this work was to study the antimicrobial susceptibility profile of S. equi from Buenos Aires, Argentina. Ninety-two isolates were studied by the single disk method to PEN, cefotaxime, erythromycin (ERY), tetracycline, enrofloxacin (ENR), trimethoprim sulfamethoxazole (TMS), ciprofloxacin, clindamycin (CLI), streptomycin (STR) and florfenicol...
November 30, 2017: Revista Argentina de Microbiología
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