Yuchen Sun, Shin-Ichiro Nitta, Kosuke Saito, Ryuta Hosogai, Keiko Nakai, Ryoya Goda, Masaaki Kakehi, Kazuyuki Murata, Takeru Yamaguchi, Takeshi Okuzono, Shinichi Yamane, Yoshimasa Enoki, Mitsuhiko Kawabata, Kentaro Takahara, Shimpei Sato, Tokuyuki Yoshida, Takao Inoue, Yoshiro Saito
Background: Ion-pairing reverse-phase LC coupled with high-resolution mass spectrometry (IP-LC/HRMS) has gained attention in oligonucleotide therapeutic bioanalyses owing to its high sensitivity and selectivity. However, optimization and validation of IP-LC/HRMS-based methods are rare. The objective of this study is the development of a sensitive and reproducible IP-LC/HRMS-based bioanalytical method using clinically approved mipomersen as a model for antisense oligonucleotides. Materials & methods/results: Mipomersen was extracted from rat plasma using Clarity OTX SPE and quantified by IP-LC/HRMS...
December 2020: Bioanalysis