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Mammary stem cell

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https://www.readbyqxmd.com/read/28734180/bottom-up-assembly-of-salivary-gland-microtissues-for-assessing-myoepithelial-cell-function
#1
Tugba Ozdemir, Padma Pradeepa Srinivasan, Daniel R Zakheim, Daniel A Harrington, Robert L Witt, Mary C Farach-Carson, Xinqiao Jia, Swati Pradhan-Bhatt
Myoepithelial cells are flat, stellate cells present in exocrine tissues including the salivary glands. While myoepithelial cells have been studied extensively in mammary and lacrimal gland tissues, less is known of the function of myoepithelial cells derived from human salivary glands. Several groups have isolated tumorigenic myoepithelial cells from cancer specimens, however, only one report has demonstrated isolation of normal human salivary myoepithelial cells needed for use in salivary gland tissue engineering applications...
July 14, 2017: Biomaterials
https://www.readbyqxmd.com/read/28722778/breast-cancer-stem-cells-are-involved-in-trastuzumab-resistance-through-the-her2-modulation-in-3d-culture
#2
Cristina Elisa Rodríguez, Damian Emilio Berardi, Marianela Abrigo, Laura Beatriz Torado, Elisa Dora Bal de Kier Joffé, Gabriel León Fiszman
Breast cancer human cells culture as spheroids develop autophagy and apoptosis, which promotes Trastuzumab resistance in HER2 overexpressing cells. Our aim was to study the association of the hostile environment developed in 3D with the breast cancer stem cells population and the HER2 modulation. Human mammary adenocarcinoma cell lines were cultured as spheroids using the hanging drop method. We generated hypoxia conditions by using a hypoxic chamber and CoCl2 treatment. Breast cancer stem cells were measured with mammosphere assays, the analysis of CD44 + CD24(low) population by flow cytometry and the pluripotent gene expression by RT-qPCR...
July 19, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28707729/sox11-promotes-invasive-growth-and-dcis-progression
#3
Erik Oliemuller, Naoko Kogata, Philip Bland, Divya Kriplani, Frances Daley, Syed Haider, Vandna Shah, Elinor J Sawyer, Beatrice A Howard
Here, we show that SOX11, an embryonic mammary marker, normally silent in postnatal breast cells, is expressed in many ER-negative (ER-) preinvasive DCIS lesions. Mature mammary epithelial cells engineered to express SOX11 displayed alterations in progenitor cell populations, including an expanded basal-like population with increased ALDH activity, and increased mammosphere-forming capacity. DCIS.com cells engineered to express SOX11 displayed increased ALDH activity, a feature of cancer stem cells. The CD44+/CD24-/ALDH+ cell population was increased in DCIS...
July 14, 2017: Journal of Pathology
https://www.readbyqxmd.com/read/28698625/ectodysplasin-target-gene-fgf20-regulates-mammary-bud-growth-and-ductal-invasion-and-branching-during-puberty
#4
Teresa Elo, Päivi H Lindfors, Qiang Lan, Maria Voutilainen, Ewelina Trela, Claes Ohlsson, Sung-Ho Huh, David M Ornitz, Matti Poutanen, Beatrice A Howard, Marja L Mikkola
Mammary gland development begins with the appearance of epithelial placodes that invaginate, sprout, and branch to form small arborized trees by birth. The second phase of ductal growth and branching is driven by the highly invasive structures called terminal end buds (TEBs) that form at ductal tips at the onset of puberty. Ectodysplasin (Eda), a tumor necrosis factor-like ligand, is essential for the development of skin appendages including the breast. In mice, Eda regulates mammary placode formation and branching morphogenesis, but the underlying molecular mechanisms are poorly understood...
July 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28693187/salinomycin-inhibits-canine-mammary-carcinoma-in-vitro-by-targeting-cancer-stem-cells
#5
Hongchao Du, Bin Zhou, Hong Zhang, Yipeng Jin, Di Zhang, Degui Lin
Salinomycin (SAL), a polyether ionophore antibiotic, has been demonstrated to selectively kill cancer stem cells (CSCs) in various types of human tumor. The aim of the present study was to investigate the effects of SAL on canine mammary CSCs. CSCs in canine mammary carcinoma cell lines (CMT7364 and CIPp) were identified using a sphere formation assay and flow cytometry. The chemoresistance, invasive potential and expression of stem cell-associated proteins of these spheres was then analyzed. This demonstrated that the spheres exhibited characteristics of CSCs, including a cluster of differentiation (CD)44(+)/CD24(-/low) phenotype, upregulation of Wnt/β-catenin signaling pathway-associated proteins and chemoresistance...
July 2017: Oncology Letters
https://www.readbyqxmd.com/read/28649656/lgr5-is-a-marker-for-fetal-mammary-stem-cells-but-is-not-essential-for-stem-cell-activity-or-tumorigenesis
#6
Christy L Trejo, Gidsela Luna, Christopher Dravis, Benjamin T Spike, Geoffrey M Wahl
The search for the bipotent mammary stem cells that drive mammary development requires markers to enable their prospective isolation. There is general agreement that bipotent mouse mammary stem cells are abundant in late fetal development, but their existence in the adult is vigorously debated. Among markers useful for mammary stem cell identification, the Wnt co-receptor Lgr5 has been suggested by some to be both "necessary and sufficient" for bipotency and transplantation of adult mammary stem cell activity, though other studies disagree...
2017: NPJ Breast Cancer
https://www.readbyqxmd.com/read/28649654/cell-state-plasticity-stem-cells-emt-and-the-generation-of-intra-tumoral-heterogeneity
#7
Geoffrey M Wahl, Benjamin T Spike
Cellular heterogeneity in cancer represents a significant challenge. In order to develop effective and lasting therapies, it is essential to understand the source of this heterogeneity, and its role in tumor progression and therapy resistance. Here, we consider not only genetic and epigenetic mechanisms, but also inflammation and cell state reprogramming in creating tumor heterogeneity. We discuss similarities between normal mammary epithelial developmental states and various breast cancer molecular sub-types, and the cells that are thought to propagate them...
2017: NPJ Breast Cancer
https://www.readbyqxmd.com/read/28634940/3d-primary-culture-model-to-study-human-mammary-development
#8
Daniel H Miller, Ethan S Sokol, Piyush B Gupta
We present a protocol for expanding human mammary tissues from primary patient-derived cells in three-dimensional (3D) cultures. The primary epithelial cells are seeded into 3D hydrogels with defined components, which include both proteins and carbohydrates present in mammary tissue. Over a span of 10-14 days, the seeded cells form mammary tissues with complex ductal-lobular topologies and include luminal and basal cells in the correct orientation, together with cells that stain positively for stem cell markers...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28624497/new-paradigms-for-the-hedgehog-signaling-network-in-mammary-gland-development-and-breast-cancer
#9
REVIEW
Teresa Monkkonen, Michael T Lewis
The Hedgehog signaling network regulates organogenesis, cell fate, proliferation, survival, and stem cell self-renewal in many mammalian tissues. Aberrant activation of the Hedgehog signaling network is present in ~25% of all cancers, including breast. Altered expression of Hedgehog network genes in the mammary gland can elicit phenotypes at many stages of development. However, synthesizing a cohesive mechanistic model of signaling at different stages of development has been difficult. Emerging data suggest that this difficulty is due, in part, to non-canonical and tissue compartment-specific (i...
June 15, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28622693/release-of-her2-repression-of-trefoil-factor-3-tff3-expression-mediates-trastuzumab-resistance-in-her2-er-mammary-carcinoma
#10
Qing-Yun Chong, Ming-Liang You, Vijay Pandey, Arindam Banerjee, Yi-Jun Chen, Han-Ming Poh, Mengyi Zhang, Lan Ma, Tao Zhu, Basappa, Liang Liu, Peter E Lobie
HER2+/ER+ breast cancer, a subset of the luminal B subtype, makes up approximately 10% of all breast cancers. The bidirectional crosstalk between HER2 and estrogen receptor (ER) in HER2+/ER+ breast cancer contributes to resistance towards both anti-estrogens and HER2-targeted therapies. TFF3 promotes breast cancer progression and has been implicated in anti-estrogen resistance in breast cancer. Herein, we investigated the cross-regulation between HER2 and estrogen-responsive TFF3, and the role of TFF3 in mediating trastuzumab resistance in HER2+/ER+ breast cancer...
June 9, 2017: Oncotarget
https://www.readbyqxmd.com/read/28607919/the-mammary-stem-cell-field-wakes-up-to-hibernating-cells
#11
EDITORIAL
Ewa M Michalak
No abstract text is available yet for this article.
2017: Stem Cell Investigation
https://www.readbyqxmd.com/read/28605392/transfer-of-mammary-gland-forming-ability-between-mammary-basal-epithelial-cells-and-mammary-luminal-cells-via-extracellular-vesicles-exosomes
#12
Meng-Chieh Lin, Shih-Yin Chen, Pei-Lin He, Wen-Ting Luo, Hua-Jung Li
Cells can communicate via exosomes, ~100-nm extracellular vesicles (EVs) that contain proteins, lipids, and nucleic acids. Non-adherent/mesenchymal mammary epithelial cell (NAMEC)-derived extracellular vesicles can be isolated from NAMEC medium via differential ultracentrifugation. Based on their density, EVs can be purified via ultracentrifugation at 110,000 x g. The EV preparation from ultracentrifugation can be further separated using a continuous density gradient to prevent contamination with soluble proteins...
June 3, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28604763/proline-rich-homeodomain-protein-prh-hhex-is-a-suppressor-of-breast-tumour-growth
#13
R M Kershaw, D Roberts, J Wragg, A M Shaaban, E Humphreys, J Halsall, L Price, R Bicknell, K Gaston, P-S Jayaraman
Breast tumours progress from hyperplasia to ductal carcinoma in situ (DCIS) and invasive breast carcinoma (IBC). PRH/HHEX (proline-rich homeodomain/haematopoietically expressed homeobox) is a transcription factor that displays both tumour suppressor and oncogenic activity in different disease contexts; however, the role of PRH in breast cancer is poorly understood. Here we show that nuclear localization of the PRH protein is decreased in DCIS and IBC compared with normal breast. Our previous work has shown that PRH phosphorylation by protein kinase CK2 prevents PRH from binding to DNA and regulating the transcription of multiple genes encoding growth factors and growth factor receptors...
June 12, 2017: Oncogenesis
https://www.readbyqxmd.com/read/28594912/cancer-cell-redirection-biomarker-discovery-using-a-mutual-information-approach
#14
Kimberly Roche, F Alex Feltus, Jang Pyo Park, Marie-May Coissieux, Chenyan Chang, Vera B S Chan, Mohamed Bentires-Alj, Brian W Booth
Introducing tumor-derived cells into normal mammary stem cell niches at a sufficiently high ratio of normal to tumorous cells causes those tumor cells to undergo a change to normal mammary phenotype and yield normal mammary progeny. This phenomenon has been termed cancer cell redirection. We have developed an in vitro model that mimics in vivo redirection of cancer cells by the normal mammary microenvironment. Using the RNA profiling data from this cellular model, we examined high-level characteristics of the normal, redirected, and tumor transcriptomes and found the global expression profiles clearly distinguish the three expression states...
2017: PloS One
https://www.readbyqxmd.com/read/28579392/bptf-maintains-chromatin-accessibility-and-the-self-renewal-capacity-of-mammary-gland-stem-cells
#15
Wesley D Frey, Anisha Chaudhry, Priscila F Slepicka, Adam M Ouellette, Steven E Kirberger, William C K Pomerantz, Gregory J Hannon, Camila O Dos Santos
Chromatin remodeling is a key requirement for transcriptional control of cellular differentiation. However, the factors that alter chromatin architecture in mammary stem cells (MaSCs) are poorly understood. Here, we show that BPTF, the largest subunit of the NURF chromatin remodeling complex, is essential for MaSC self-renewal and differentiation of mammary epithelial cells (MECs). BPTF depletion arrests cells at a previously undefined stage of epithelial differentiation that is associated with an incapacity to achieve the luminal cell fate...
July 11, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28573159/high-fat-diet-induced-breast-cancer-model-in-rat
#16
Meng-Ju Wu, Chun-Ju Chang
Obesity has been linked to breast cancer progression but the underlying mechanisms remain obscure. Being overweight or obese for a woman at the time she is diagnosed with breast cancer is linked to a high risk of recurrence regardless of treatment factors. In rodents, high body weight is also associated with increased incidence of spontaneous and chemically induced tumors. To study the complex interaction between the mammary epithelia and the microenvironment, with a focus on the mechanism underlying the role obesity plays in the regulation of the cancer stem cell traits and the development of mammary cancer in vivo, we have established a diet-induced obesity (DIO) rat model of breast cancer (Chang et al...
July 5, 2016: Bio-protocol
https://www.readbyqxmd.com/read/28530657/normal-and-cancerous-mammary-stem-cells-evade-interferon-induced-constraint-through-the-mir-199a-lcor-axis
#17
Toni Celià-Terrassa, Daniel D Liu, Abrar Choudhury, Xiang Hang, Yong Wei, Jose Zamalloa, Raymundo Alfaro-Aco, Rumela Chakrabarti, Yi-Zhou Jiang, Bong Ihn Koh, Heath A Smith, Christina DeCoste, Jun-Jing Li, Zhi-Ming Shao, Yibin Kang
Tumour-initiating cells, or cancer stem cells (CSCs), possess stem-cell-like properties observed in normal adult tissue stem cells. Normal and cancerous stem cells may therefore share regulatory mechanisms for maintaining self-renewing capacity and resisting differentiation elicited by cell-intrinsic or microenvironmental cues. Here, we show that miR-199a promotes stem cell properties in mammary stem cells and breast CSCs by directly repressing nuclear receptor corepressor LCOR, which primes interferon (IFN) responses...
June 2017: Nature Cell Biology
https://www.readbyqxmd.com/read/28499884/inhibition-of-ubiquitin-specific-protease-34-usp34-induces-epithelial-mesenchymal-transition-and-promotes-stemness-in-mammary-epithelial-cells
#18
Eunhye Oh, Ji Young Kim, Daeil Sung, Youngkwan Cho, Nahyun Lee, Hyunsook An, Yoon-Jae Kim, Tae-Min Cho, Jae Hong Seo
Ubiquitin-specific protease 34 (USP34) is a deubiquitinating enzyme that regulates Axin stability and plays a critical role in Wnt/β-catenin signaling. We sought to investigate the role of USP34 on epithelial-mesenchymal (EMT) induction and its effects on mammary epithelial stem cells. USP34 expression levels were relatively lower in MDA-MB-231 and 4T1 mesenchymal-like cells when compared to epithelial-like cells. Inhibition of USP34 in NMuMG cells induced EMT, as evidenced by the upregulation of EMT markers including N-cadherin, phospho-Smad3, Snail and active-β-catenin, as well as the downregulation of Axin 1 and E-cadherin...
May 10, 2017: Cellular Signalling
https://www.readbyqxmd.com/read/28499392/experimental-tumor-growth-of-canine-osteosarcoma-cell-line-on-chick-embryo-chorioallantoic-membrane-in-vivo-studies
#19
Magdalena Walewska, Izabella Dolka, Anna Małek, Anna Wojtalewicz, Agata Wojtkowska, Artur Żbikowski, Roman Lechowski, Katarzyna Zabielska-Koczywąs
The chick embryo chorioallantoic membrane (CAM) model is extensively used in human medicine in preclinical oncological studies. The CAM model has several advantages: low cost, simple experimental approach, time saving and following "3R principles". Research has shown that the human osteosarcoma cell lines U2OS, MMNG-HOS, and SAOS can form tumors on the CAM. In veterinary medicine, this has been described only for feline fibrosarcomas, feline mammary carcinomas and canine osteosarcomas. However, in case of canine osteosarcomas, it has been shown that only non-adherent osteosarcoma stem cells isolated from KTOSA5 and CSKOS cell lines have the ability to form microtumors on the CAM after an incubation period of 5 days, in contrast to adherent KTOSA5 and CSKOS cells...
May 12, 2017: Acta Veterinaria Scandinavica
https://www.readbyqxmd.com/read/28498248/c1galt1-seems-to-promote-in-vitro-disease-progression-in-ovarian-cancer
#20
Chih-Hsing Chou, Miao-Juei Huang, Ying-Yu Liao, Chi-Hau Chen, Min-Chuan Huang
OBJECTIVE: Aberrant glycosylation affects many cellular properties in cancers. The core 1 β1,3-galactosyltransferase (C1GALT1), an enzyme that controls the formation of mucin-type O-glycans, has been reported to regulate hepatocellular and mammary carcinogenesis. This study aimed to explore the role of C1GALT1 in ovarian cancer. METHODS: C1GALT1 expression was assessed in a public database based on microarray data from 1287 ovarian cancer patients and ovarian cancerous tissues...
June 2017: International Journal of Gynecological Cancer
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