keyword
MENU ▼
Read by QxMD icon Read
search

Mammary stem cell

keyword
https://www.readbyqxmd.com/read/27895151/high-fat-diet-promotes-mammary-gland-myofibroblast-differentiation-through-mir-140-downregulation
#1
Benjamin Wolfson, Yongshu Zhang, Ramkishore Gernapudi, Nadire Duru, Yuan Yao, Pang-Kuo Lo, Qun Zhou
Human breast adipose tissue is a heterogonous cell population consisting of mature white adipocytes, multipotent mesenchymal stem cells, committed progenitor cells, fibroblasts, endothelial cells, and immune cells. Dependent on external stimulation, adipose derived stem cells differentiate along diverse lineages into adipocytes, chondrocytes, osteoblasts, fibroblasts, and myofibroblasts. It is currently not fully understood how high-fat diet reprograms adipose-derived stem cells into myofibroblasts. In our study, we use regular-diet and high-fat diet induced obesity mouse models to investigate the role of dietary fat on myofibroblast differentiation in the mammary stromal microenvironment...
November 28, 2016: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/27894093/new-use-of-an-old-drug-inhibition-of-breast-cancer-stem-cells-by-benztropine-mesylate
#2
Jihong Cui, Maija Hollmén, Lina Li, Yong Chen, Steven T Proulx, Daniel Reker, Gisbert Schneider, Michael Detmar
Cancer stem cells (CSCs) play major roles in cancer initiation, metastasis, recurrence and therapeutic resistance. Targeting CSCs represents a promising strategy for cancer treatment. The purpose of this study was to identify selective inhibitors of breast CSCs (BCSCs). We carried out a cell-based phenotypic screening with cell viability as a primary endpoint, using a collection of 2,546 FDA-approved drugs and drug-like molecules in spheres formed by malignant human breast gland-derived cells (HMLER-shEcad cells, representing BCSCs) and control immortalized non-tumorigenic human mammary cells (HMLE cells, representing normal stem cells)...
November 24, 2016: Oncotarget
https://www.readbyqxmd.com/read/27886379/supernatants-of-adipocytes-from-obese-vs-normal-weight-women-and-breast-cancer-cells-in-vitro-impact-on-angiogenesis
#3
L Bougaret, L Delort, H Billard, C Lequeux, N Goncalves-Mendes, A Mojallal, O Damour, M-P Vasson, F Caldefie-Chezet
Breast cancer is correlated with a higher risk of metastasis in obese postmenopausal women. Adipokines, whose plasma concentrations are modulated in obese subjects, surrounds mammary cells, suggesting that adipocyte secretions affect mammary tumorogenesis. We hypothsesis that matures adipocytes secretions from obese women conditioned or not by breast neoplasic cells, increase changes on the stages of angiogenesis. Supernatants of human matures adipocytes differentiated from stem cells of either adipose tissue of normal weight (MA20) or obese (MA30) women or obtained from co-cultures between MA20 or MA30 and breast cancer cell line MCF-7 were collected...
November 25, 2016: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/27881737/rankl-rank-from-bone-loss-to-the-prevention-of-breast-cancer
#4
REVIEW
Verena Sigl, Laundette P Jones, Josef M Penninger
RANK and RANKL, a receptor ligand pair belonging to the tumour necrosis factor family, are the critical regulators of osteoclast development and bone metabolism. Besides their essential function in bone, RANK and RANKL have also been identified as the key factors for the formation of a lactating mammary gland in pregnancy. Mechanistically, RANK and RANKL link the sex hormone progesterone with stem cell expansion and proliferation of mammary epithelial cells. Based on their normal physiology, RANKL/RANK control the onset of hormone-induced breast cancer through the expansion of mammary progenitor cells...
November 2016: Open Biology
https://www.readbyqxmd.com/read/27872203/molecular-mechanisms-of-asymmetric-divisions-in-mammary-stem-cells
#5
REVIEW
Angela Santoro, Thalia Vlachou, Manuel Carminati, Pier Giuseppe Pelicci, Marina Mapelli
Stem cells have the remarkable ability to undergo proliferative symmetric divisions and self-renewing asymmetric divisions. Balancing of the two modes of division sustains tissue morphogenesis and homeostasis. Asymmetric divisions of Drosophila neuroblasts (NBs) and sensory organ precursor (SOP) cells served as prototypes to learn what we consider now principles of asymmetric mitoses. They also provide initial evidence supporting the notion that aberrant symmetric divisions of stem cells could correlate with malignancy...
November 21, 2016: EMBO Reports
https://www.readbyqxmd.com/read/27871860/in%C3%A2-vitro-reconstitution-of-breast-cancer-heterogeneity-with-multipotent-cancer-stem-cells-using-small-molecules
#6
Masaki Kawamata, Takeshi Katsuda, Yasuhiro Yamada, Takahiro Ochiya
A small fraction of tumor cells are thought to possess the potential for both multiple-lineage differentiation and self-renewal, which underlies the cancer stem cell hypothesis. However, the differentiation mechanisms of these cells have not been elucidated due to a lack of appropriate culture methods. Here, we established a culture condition for maintaining multipotent tumor cells from rat breast tumors using 4 small molecules. Cultured tumor cells in this condition retained their intrinsic myoepithelial features, expressing p63 and CK14 and vimentin...
November 18, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27869165/tap63-suppresses-mammary-tumorigenesis-through-regulation-of-the-hippo-pathway
#7
X Su, M Napoli, H A Abbas, A Venkatanarayan, N H B Bui, C Coarfa, Y J Gi, F Kittrell, P H Gunaratne, D Medina, J M Rosen, F Behbod, E R Flores
Mechanisms regulating the transition of mammary epithelial cells (MECs) to mammary stem cells (MaSCs) and to tumor-initiating cells (TICs) have not been entirely elucidated. The p53 family member, p63, is critical for mammary gland development and contains transactivation domain isoforms, which have tumor-suppressive activities, and the ΔN isoforms, which act as oncogenes. In the clinic, p63 is often used as a diagnostic marker, and further analysis of the function of TAp63 in the mammary gland is critical for improved diagnosis and patient care...
November 21, 2016: Oncogene
https://www.readbyqxmd.com/read/27866304/cell-lineage-determinants-as-regulators-of-breast-cancer-metastasis
#8
Wei Lu, Yibin Kang
The mammary epithelium is organized in a hierarchy of mammary stem cells (MaSCs), progenitors, and differentiated cells. The development and homeostasis of mammary gland are tightly controlled by a complex network of cell lineage regulators. These determinants of cellular hierarchy are frequently deregulated in breast tumor cells and closely associated with cancer progression and metastasis. They also contribute to the diversity of breast cancer subtypes and their distinct metastatic patterns. Cell fate regulators that normally promote stem/progenitor activities can serve as drivers for epithelial-mesenchymal transition and metastasis whereas regulators that promote terminal differentiation generally suppress metastasis...
November 19, 2016: Cancer Metastasis Reviews
https://www.readbyqxmd.com/read/27852980/aging-is-associated-with-an-expansion-of-cd49f-hi-mammary-stem-cells-that-show-a-decline-in-function-and-increased-transformation-potential
#9
Qiaoxiang Dong, Hui Gao, Yuanshuo Shi, Fuchuang Zhang, Xiang Gu, Anqi Wu, Danhan Wang, Yuanhong Chen, Abhik Bandyopadhyay, I-Tien Yeh, Benjamin J Daniel, Yidong Chen, Yi Zou, Vivienne L Rebel, Christi A Walter, Jianxin Lu, Changjiang Huang, Lu-Zhe Sun
Breast cancer incidence increases during aging, yet the mechanism of age-associated mammary tumorigenesis is unclear. Mammary stem cells are believed to play an important role in breast tumorigenesis, but how their function changes with age is unknown. We compared mammary epithelial cells isolated from young and old mammary glands of different cohorts of C57BL6/J and BALB/c mice, and our findings revealed that old mammary glands were characterized by increased basal cell pool comprised of mostly CD49f(hi) cells, altered luminal-to-basal cell ratio, and irregular ductal morphology...
November 15, 2016: Aging
https://www.readbyqxmd.com/read/27820703/sinusoidal-ephrin-receptor-ephb4-controls-hematopoietic-progenitor-cell-mobilization-from-bone-marrow
#10
Hyeongil Kwak, Ombretta Salvucci, Roberto Weigert, Jorge L Martinez-Torrecuadrada, Mark Henkemeyer, Michael G Poulos, Jason M Butler, Giovanna Tosato
Hematopoietic stem and progenitor cells (HSPCs) reside in the bone marrow. Stress signals from cancer and other conditions promote HSPC mobilization into circulation and subsequent homing to tissue microenvironments. HSPC infiltration into tissue microenvironments can influence disease progression; notably, in cancer, HSPCs encourage tumor growth. Here we have uncovered a mutually exclusive distribution of EPHB4 receptors in bone marrow sinusoids and ephrin B2 ligands in hematopoietic cells. We determined that signaling interactions between EPHB4 and ephrin B2 control HSPC mobilization from the bone marrow...
November 7, 2016: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/27807451/lifespan-extension-and-sustained-expression-of-stem-cell-phenotype-of-human-breast-epithelial-stem-cells-in-a-medium-with-antioxidants
#11
Kai-Hung Wang, An-Pei Kao, Chia-Cheng Chang, Ta-Chin Lin, Tsung-Cheng Kuo
We have previously reported the isolation and culture of a human breast epithelial cell type with stem cell characteristics (Type I HBEC) from reduction mammoplasty using the MSU-1 medium. Subsequently, we have developed several different normal human adult stem cell types from different tissues using the K-NAC medium. In this study, we determined whether this low calcium K-NAC medium with antioxidants (N-acetyl-L-cysteine and L-ascorbic acid-2-phosphate) is a better medium to grow human breast epithelial cells...
2016: Stem Cells International
https://www.readbyqxmd.com/read/27806317/resveratrol-changes-spermatogonial-stem-cells-sscs-activity-and-ameliorates-their-loss-in-busulfan-induced-infertile-mouse
#12
Chongyang Wu, Ying Zhang, Qiaoyan Shen, Zhe Zhou, Weishuai Liu, Jinlian Hua
The decline of quantity and quality of sperm are correlated with the increasing age and some anti-cancer compounds such as busulfan. Previous studies have shown that Resveratrol (Res) inhibits tumorigenesis and metastasis of many cancers including mammary tumor, prostate and pancreatic cancers. It acts as anti-age in mouse and human, however, little is known about its protective effect on aged spermatogonial stem cells (SSCs). Here, we investigated the effects of Res in vitro on SSCs usingC18-4 cells and in vivo in busulfan-induced azoospermia mice model...
October 29, 2016: Oncotarget
https://www.readbyqxmd.com/read/27798604/lgr6-labels-a-rare-population-of-mammary-gland-progenitor-cells-that-are-able-to-originate-luminal-mammary-tumours
#13
Leander Blaas, Fabio Pucci, Hendrik A Messal, Agneta B Andersson, E Josue Ruiz, Marco Gerling, Iyadh Douagi, Bradley Spencer-Dene, Alexandra Musch, Richard Mitter, Leena Bhaw, Richard Stone, Dorothee Bornhorst, Abdul K Sesay, Jos Jonkers, Gordon Stamp, Ilaria Malanchi, Rune Toftgård, Axel Behrens
The mammary gland is composed of a complex cellular hierarchy with unusual postnatal plasticity. The identities of stem/progenitor cell populations, as well as tumour-initiating cells that give rise to breast cancer, are incompletely understood. Here we show that Lgr6 marks rare populations of cells in both basal and luminal mammary gland compartments in mice. Lineage tracing analysis showed that Lgr6(+) cells are unipotent progenitors, which expand clonally during puberty but diminish in adulthood. In pregnancy or following stimulation with ovarian hormones, adult Lgr6(+) cells regained proliferative potency and their progeny formed alveoli over repeated pregnancies...
October 31, 2016: Nature Cell Biology
https://www.readbyqxmd.com/read/27797378/stromal-pten-inhibits-the-expansion-of-mammary-epithelial-stem-cells-through-jagged-1
#14
G M Sizemore, S Balakrishnan, A M Hammer, K A Thies, A J Trimboli, J A Wallace, S T Sizemore, R D Kladney, S A Woelke, L Yu, S A Fernandez, A Chakravarti, G Leone, M C Ostrowski
Fibroblasts within the mammary tumor microenvironment are active participants in carcinogenesis mediating both tumor initiation and progression. Our group has previously demonstrated that genetic loss of phosphatase and tensin homolog (PTEN) in mammary fibroblasts induces an oncogenic secretome that remodels the extracellular milieu accelerating ErbB2-driven mammary tumor progression. While these prior studies highlighted a tumor suppressive role for stromal PTEN, how the adjacent normal epithelium transforms in response to PTEN loss was not previously addressed...
October 31, 2016: Oncogene
https://www.readbyqxmd.com/read/27796960/lineage-tracing-of-mammary-stem-and-progenitor-cells
#15
Anoeska A A van de Moosdijk, Nai Yang Fu, Anne C Rios, Jane E Visvader, Renée van Amerongen
Lineage tracing analysis allows mammary epithelial cells to be tracked in their natural environment, thereby revealing cell fate and proliferation choices in the intact tissue. This technique is particularly informative for studying how stem cells build and maintain the mammary epithelium during development and pregnancy. Here we describe two experimental systems based on Cre/loxP technology (Cre(ERT2)/loxP and rtTA/tetO-Cre/loxP), which allow the inducible, permanent labeling of mammary epithelial cells following the administration of either tamoxifen or doxycycline...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27796959/techniques-for-the-reprogramming-of-exogenous-stem-progenitor-cell-populations-towards-a-mammary-epithelial-cell-fate
#16
Gilbert H Smith, Corinne A Boulanger
This chapter considers the techniques necessary and required for the reprogramming of exogenous stem/progenitor cell populations towards a mammary epithelial cell fate. The protocols describe how to isolate cells from alternate mouse organs such as testicles of male mice and mix them with mammary cells to generate chimeric glands comprised of male and female epithelial cells that are fully competent. During the reformation of mammary stem cell niches by dispersed epithelial cells, in the context of the intact epithelium-free mammary stroma, non-mammary cells are sequestered and reprogrammed to perform mammary epithelial cell functions including those ascribed to mammary stem/progenitor cells...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27793013/mammary-gland-stem-cells-and-their-application-in-breast-cancer
#17
REVIEW
Xing Yang, Hui Wang, Baowei Jiao
The mammary gland is an organ comprising two primary lineages, specifically the inner luminal and the outer myoepithelial cell layers. Mammary gland stem cells (MaSCs) are highly dynamic and self-renewing, and can give rise to these mammary gland lineages. The lineages are responsible for gland generation during puberty as well as expansion during pregnancy. In recent years, researchers have focused on understanding how MaSCs are regulated during mammary gland development and transformation of breast cancer...
October 25, 2016: Oncotarget
https://www.readbyqxmd.com/read/27782124/wnt-and-neuregulin1-erbb-signalling-extends-3d-culture-of-hormone-responsive-mammary-organoids
#18
Thierry Jardé, Bethan Lloyd-Lewis, Mairian Thomas, Howard Kendrick, Lorenzo Melchor, Lauriane Bougaret, Peter D Watson, Kenneth Ewan, Matthew J Smalley, Trevor C Dale
The development of in vitro culture systems quantitatively and qualitatively recapitulating normal breast biology is key to the understanding of mammary gland biology. Current three-dimensional mammary culture systems have not demonstrated concurrent proliferation and functional differentiation ex vivo in any system for longer than 2 weeks. Here, we identify conditions including Neuregulin1 and R-spondin 1, allowing maintenance and expansion of mammary organoids for 2.5 months in culture. The organoids comprise distinct basal and luminal compartments complete with functional steroid receptors and stem/progenitor cells able to reconstitute a complete mammary gland in vivo...
October 26, 2016: Nature Communications
https://www.readbyqxmd.com/read/27779470/tumour-associated-macrophages-influence-canine-mammary-cancer-stem-like-cells-enhancing-their-pro-angiogenic-properties
#19
A Rybicka, C Eyileten, B Taciak, J Mucha, K Majchrzak, E Hellmen, M Krol
Cancer stem-like cells as cells with ability to self-renewal and potential to differentiate into various types of cells are known to be responsible for tumour initiation, recurrence and drug resistance. Hence a comprehensive research is concentrated on discovering cancer stem-like cells biology and interdependence between them and other cells. The aim of our study was to evaluate the impact of macrophages on cancer stem-like cells in canine mammary carcinomas. As recent studies indicated presence of macrophages in cancer environment stimulates cancer cells into more motile and invasive cells by acquisition of macrophage phenotypes...
August 2016: Journal of Physiology and Pharmacology: An Official Journal of the Polish Physiological Society
https://www.readbyqxmd.com/read/27779190/single-cell-lineage-tracing-in-the-mammary-gland-reveals-stochastic-clonal-dispersion-of-stem-progenitor-cell-progeny
#20
Felicity M Davis, Bethan Lloyd-Lewis, Olivia B Harris, Sarah Kozar, Douglas J Winton, Leila Muresan, Christine J Watson
The mammary gland undergoes cycles of growth and regeneration throughout reproductive life, a process that requires mammary stem cells (MaSCs). Whilst recent genetic fate-mapping studies using lineage-specific promoters have provided valuable insights into the mammary epithelial hierarchy, the true differentiation potential of adult MaSCs remains unclear. To address this, herein we utilize a stochastic genetic-labelling strategy to indelibly mark a single cell and its progeny in situ, combined with tissue clearing and 3D imaging...
October 25, 2016: Nature Communications
keyword
keyword
18784
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"