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https://www.readbyqxmd.com/read/28730439/design-and-preparation-of-aptamer-sirna-chimeras-asics-for-targeted-cancer-therapy
#1
Sven Kruspe, Paloma H Giangrande
Conjugation of cell-internalizing DNA or RNA aptamers to tumor-suppressing siRNAs represents a novel promising approach for cancer therapy. Here we describe how to employ RNA aptamers that bind to cell surface receptors as carriers for cell-targeted siRNA (or miRNA) delivery. This protocol was optimized to improve the efficiency of the aptamer-siRNA/miRNA conjugates and facilitate its implementation in most molecular biology labs. The single working steps include (1) outlining the optimal sequences of the RNA strands bearing the aptamer and siRNA sequence, for which further (2) a dsDNA template is synthesized and then (3) transcribed into an RNA that will be (4) folded and annealed to a chemically synthesized siRNA complementary strand...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28728821/the-kinetics-and-the-permeation-properties-of-piezo-channels
#2
R Gnanasambandam, P A Gottlieb, F Sachs
Piezo channels are eukaryotic, cation-selective mechanosensitive channels (MSCs), which show rapid activation and voltage-dependent inactivation. The kinetics of these channels are largely consistent across multiple cell types and different stimulation paradigms with some minor variability. No accessory subunits that associate with Piezo channels have been reported. They are homotrimers and each ∼300kD monomer has an N-terminal propeller blade-like mechanosensing module, which can confer mechanosensing capabilities on ASIC-1 (the trimeric non-MSC, acid-sensing ion channel-1) and a C-terminal pore module, which influences conductance, selectivity, and channel inactivation...
2017: Current Topics in Membranes
https://www.readbyqxmd.com/read/28693714/psychometric-properties-of-the-spanish-version-of-the-anxiety-sensitivity-index-for-children
#3
José Fernández-Valdés, Aurora Gavino, Lidia Valderrama-Martos, Antonio Godoy, Jeff Laurent
BACKGROUND: Anxiety sensitivity is a useful construct in explaining anxiety disorders. The Anxiety Sensitivity Index for Children (ASIC) is a brief self-report designed to assess anxiety sensitivity in youth. METHOD: Students (N = 1348; ages 10-17) completed a Spanish translation of the ASIC and measures of anxiety and depression. RESULTS: Confirmatory factor analyses found that scores on the ASIC- Spanish version can be fitted to two factorial structures: A single factor and two first-order factors grouped into one higher-order factor...
August 2017: Psicothema
https://www.readbyqxmd.com/read/28688766/ligands-of-histamine-receptors-modulate-acid-sensing-ion-channels
#4
V Y Shteinikov, A S Korosteleva, T B Tikhonova, N N Potapieva, D B Tikhonov
Recently we found that synthetic compounds containing amino group linked to hydrophobic or aromatic moiety are potent modulators of the proton-gated channels (ASICs). These structures have clear similarity with ligands of histamine receptors. We have also demonstrated that histamine potentiates homomeric ASIC1a by shifting its activation dependence to less acidic conditions. In the present work the action of a series of histamine receptors ligands on recombinant ASIC1a and ASIC2a was characterized. Two types of action were found for ASIC1a...
July 5, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28674755/modulation-of-the-fmrfamide-gated-na-channel-by-external-ca-2
#5
Akihiko Fujimoto, Yu Kodani, Yasuo Furukawa
FMRFamide-gated Na(+) channel (FaNaC) is a member of the DEG/ENaC family. Amino acid sequence of the second transmembrane region (TM2) of FaNaC is quite similar to that of the acid-sensing ion channels (ASIC) of the same family. In the upper part of TM2, there are two aspartate residues (D552 and D556 in Aplysia FaNaC, AkFaNaC) which construct two negative rings in the external vestibule. In the present study, we examined the function of D552/D556 mutants of AkFaNaC in Xenopus oocytes with special interest in Ca(2+) sensitivity of FaNaC...
July 3, 2017: Pflügers Archiv: European Journal of Physiology
https://www.readbyqxmd.com/read/28672813/a-spaceborne-synthetic-aperture-radar-partial-fixed-point-imaging-system-using-a-field-programmable-gate-array-application-specific-integrated-circuit-hybrid-heterogeneous-parallel-acceleration-technique
#6
Chen Yang, Bingyi Li, Liang Chen, Chunpeng Wei, Yizhuang Xie, He Chen, Wenyue Yu
With the development of satellite load technology and very large scale integrated (VLSI) circuit technology, onboard real-time synthetic aperture radar (SAR) imaging systems have become a solution for allowing rapid response to disasters. A key goal of the onboard SAR imaging system design is to achieve high real-time processing performance with severe size, weight, and power consumption constraints. In this paper, we analyse the computational burden of the commonly used chirp scaling (CS) SAR imaging algorithm...
June 24, 2017: Sensors
https://www.readbyqxmd.com/read/28657172/asic1a-in-neurons-is-critical-for-fear-related-behaviors
#7
R J Taugher, Y Lu, R Fan, A Ghobbeh, C J Kreple, F M Faraci, J A Wemmie
Acid-sensing ion channels (ASICs) have been implicated in fear-, addiction-, and depression-related behaviors in mice. While these effects have been attributed to ASIC1A in neurons, it has been reported that ASICs may also function in non-neuronal cells. To determine if ASIC1A in neurons is indeed required, we generated neuron-specific knockout mice with floxed Asic1a alleles disrupted by Cre recombinase driven by the neuron-specific synapsin I promoter (SynAsic1a KO mice). We confirmed that Cre expression occurred in neurons, but not all neurons, and not in non-neuronal cells including astrocytes...
June 28, 2017: Genes, Brain, and Behavior
https://www.readbyqxmd.com/read/28562320/asics-may-affect-gabaergic-synapses
#8
EDITORIAL
Maksim Storozhuk, Oleg Krishtal
No abstract text is available yet for this article.
June 27, 2017: Oncotarget
https://www.readbyqxmd.com/read/28541119/treatment-of-opioid-use-disorder-with-ibogaine-detoxification-and-drug-use-outcomes
#9
Thomas Kingsley Brown, Kenneth Alper
BACKGROUND: Ibogaine is a monoterpene indole alkaloid used in medical and nonmedical settings for the treatment of opioid use disorder. Its mechanism of action is apparently novel. There are no published prospective studies of drug use outcomes with ibogaine. OBJECTIVES: To study outcomes following opioid detoxification with ibogaine. METHODS: In this observational study, 30 subjects with DSM-IV Opioid Dependence (25 males, 5 females) received a mean total dose of 1,540 ± 920 mg ibogaine HCl...
May 25, 2017: American Journal of Drug and Alcohol Abuse
https://www.readbyqxmd.com/read/28528673/acid-sensing-ion-channel-pharmacology-past-present-and-future-%C3%A2
#10
Lachlan D Rash
pH is one of the most strictly controlled parameters in mammalian physiology. An extracellular pH of ~7.4 is crucial for normal physiological processes, and perturbations to this have profound effects on cell function. Acidic microenvironments occur in many physiological and pathological conditions, including inflammation, bone remodeling, ischemia, trauma, and intense synaptic activity. Cells exposed to these conditions respond in different ways, from tumor cells that thrive to neurons that are either suppressed or hyperactivated, often fatally...
2017: Advances in Pharmacology
https://www.readbyqxmd.com/read/28518134/asic1-and-asic3-contribute-to-acidity-induced-emt-of-pancreatic-cancer-through-activating-ca-2-rhoa-pathway
#11
Shuai Zhu, Hai-Yun Zhou, Shi-Chang Deng, Shi-Jiang Deng, Chi He, Xiang Li, Jing-Yuan Chen, Yan Jin, Zhuang-Li Hu, Fang Wang, Chun-You Wang, Gang Zhao
Extracellular acid can have important effects on cancer cells. Acid-sensing ion channels (ASICs), which emerged as key receptors for extracellular acidic pH, are differently expressed during various diseases and have been implicated in underlying pathogenesis. This study reports that ASIC1 and ASIC3 are mainly expressed on membrane of pancreatic cancer cells and upregulated in pancreatic cancer tissues. ASIC1 and ASIC3 are responsible for an acidity-induced inward current, which is required for elevation of intracellular Ca(2+) concentration ([Ca(2+)]i)...
May 18, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28516169/hybrid-pixel-waveform-hpwf-enabled-cdte-detectors-for-small-animal-gamma-ray-imaging-applications
#12
A Groll, K Kim, H Bhatia, J C Zhang, J H Wang, Z M Shen, L Cai, J Dutta, Q Li, L J Meng
This paper presents the design and preliminary evaluation of small-pixel CdTe gamma ray detectors equipped with a hybrid pixel-waveform (HPWF) readout system for gamma ray imaging applications with additional discussion on CZT due to its similarity. The HPWF readout system utilizes a pixelated anode readout circuitry which is designed to only provide the pixel address. This readout circuitry works in coincidence with a high-speed digitizer to sample the cathode waveform which provides the energy, timing, and depth-of-interaction (DOI) information...
January 2017: IEEE transactions on radiation and plasma medical sciences
https://www.readbyqxmd.com/read/28504937/ultrasonic-fingerprint-sensor-with-transmit-beamforming-based-on-a-pmut-array-bonded-to-cmos-circuitry
#13
Xiaoyue Jiang, Hao-Yen Tang, Yipeng Lu, Eldwin Ng, Julius Tsai, Bernhard Boser, David Horsley
In this paper, we present a single-chip 65x42 element ultrasonic pulse-echo fingerprint sensor with transmit beamforming based on piezoelectric micromachined ultrasonic transducers (PMUTs) directly bonded to a CMOS readout ASIC. The readout ASIC was realized in a standard 180 nm CMOS process with a 24V high voltage transistor option. Pulse-echo measurements are performed column-by-column in sequence using either one column or five columns to transmit the ultrasonic pulse at 20 MHz. Transmit beamforming is used to focus the ultrasonic beam at the imaging plane where the finger is located, increasing the ultrasonic pressure and narrowing the 3dB beam-width to 50 microns, a factor of 6...
May 11, 2017: IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control
https://www.readbyqxmd.com/read/28498103/a-selectivity-filter-at-the-intracellular-end-of-the-acid-sensing-ion-channel-pore
#14
Timothy Lynagh, Emelie Flood, Céline Boiteux, Matthias Wulf, Vitaly V Komnatnyy, Janne M Colding, Toby W Allen, Stephan A Pless
Increased extracellular proton concentrations during neurotransmission are converted to excitatory sodium influx by acid-sensing ion channels (ASICs). 10-fold sodium/potassium selectivity in ASICs has long been attributed to a central constriction in the channel pore, but experimental verification is lacking due to the sensitivity of this structure to conventional manipulations. Here, we explored the basis for ion selectivity by incorporating unnatural amino acids into the channel, engineering channel stoichiometry and performing free energy simulations...
May 12, 2017: ELife
https://www.readbyqxmd.com/read/28489547/a-172-mu-w-compressively-sampled-photoplethysmographic-ppg-readout-asic-with-heart-rate-estimation-directly-from-compressively-sampled-data
#15
Venkata Rajesh Pamula, Jose Manuel Valero-Sarmiento, Long Yan, Alper Bozkurt, Chris Van Hoof, Nick Van Helleputte, Refet Firat Yazicioglu, Marian Verhelst
A compressive sampling (CS) photoplethysmographic (PPG) readout with embedded feature extraction to estimate heart rate (HR) directly from compressively sampled data is presented. It integrates a low-power analog front end together with a digital back end to perform feature extraction to estimate the average HR over a 4 s interval directly from compressively sampled PPG data. The application-specified integrated circuit (ASIC) supports uniform sampling mode (1x compression) as well as CS modes with compression ratios of 8x, 10x, and 30x...
June 2017: IEEE Transactions on Biomedical Circuits and Systems
https://www.readbyqxmd.com/read/28472753/chemical-toxicity-and-radioactivity-of-depleted-uranium-the-evidence-from-in-vivo-and-in-vitro-studies
#16
REVIEW
Adna Asic, Amina Kurtovic-Kozaric, Larisa Besic, Lejla Mehinovic, Azra Hasic, Mirza Kozaric, Mirsada Hukic, Damir Marjanovic
The main aim of this review is to summarize and discuss the current state of knowledge on chemical toxicity and radioactivity of depleted uranium (DU) and their effect on living systems and cell lines. This was done by presenting a summary of previous investigations conducted on different mammalian body systems and cell cultures in terms of potential changes caused by either chemical toxicity or radioactivity of DU. In addition, the authors aimed to point out the limitations of those studies and possible future directions...
July 2017: Environmental Research
https://www.readbyqxmd.com/read/28461138/distribution-of-asic4-transcripts-in-the-adult-wild-type-mouse-brain
#17
M Hoshikawa, A Kato, H Hojo, Y Shibata, N Kumamoto, M Watanabe, S Ugawa
Acid-sensing ion channel 4 (ASIC4) belongs to the ASIC gene family of neuronal proton-gated cation channels, and is the least understood subtype among the members. Previous studies of ASIC4 expression in the mammalian central nervous system have shown that ASIC4 is abundantly expressed in the spinal cord and in various brain regions, such as the cerebral cortex, the hippocampus, and the cerebellum. However, the detailed distribution of ASIC4 transcripts in mammalian brains still remains to be elucidated. In the present study, radioactive in situ hybridization histochemistry with an ASIC4-specific cRNA probe was performed on wild-type mouse brains, followed by X-gal staining experiments with Asic4-lacZ reporter mice Asic4(tm1a(KOMP)Mbp)...
April 29, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28459831/parallel-point-multiplication-architecture-using-combined-group-operations-for-high-speed-cryptographic-applications
#18
Md Selim Hossain, Ehsan Saeedi, Yinan Kong
In this paper, we propose a novel parallel architecture for fast hardware implementation of elliptic curve point multiplication (ECPM), which is the key operation of an elliptic curve cryptography processor. The point multiplication over binary fields is synthesized on both FPGA and ASIC technology by designing fast elliptic curve group operations in Jacobian projective coordinates. A novel combined point doubling and point addition (PDPA) architecture is proposed for group operations to achieve high speed and low hardware requirements for ECPM...
2017: PloS One
https://www.readbyqxmd.com/read/28457973/acid-sensing-ion-channel-asic-structure-and-function-insights-from-spider-snake-and-sea-anemone-venoms
#19
REVIEW
Ben Cristofori-Armstrong, Lachlan D Rash
Acid-sensing ion channels (ASICs) are proton-activated cation channels that are expressed in a variety of neuronal and non-neuronal tissues. As proton-gated channels, they have been implicated in many pathophysiological conditions where pH is perturbed. Venom derived compounds represent the most potent and selective modulators of ASICs described to date, and thus have been invaluable as pharmacological tools to study ASIC structure, function, and biological roles. There are now ten ASIC modulators described from animal venoms, with those from snakes and spiders favouring ASIC1, while the sea anemones preferentially target ASIC3...
April 27, 2017: Neuropharmacology
https://www.readbyqxmd.com/read/28443846/on-chip-enzymatic-microbiofuel-cell-powered-integrated-circuits
#20
Andrew G Mark, Emmanuel Suraniti, Jérôme Roche, Harald Richter, Alexander Kuhn, Nicolas Mano, Peer Fischer
A variety of diagnostic and therapeutic medical technologies rely on long term implantation of an electronic device to monitor or regulate a patient's condition. One proposed approach to powering these devices is to use a biofuel cell to convert the chemical energy from blood nutrients into electrical current to supply the electronics. We present here an enzymatic microbiofuel cell whose electrodes are directly integrated into a digital electronic circuit. Glucose oxidizing and oxygen reducing enzymes are immobilized on microelectrodes of an application specific integrated circuit (ASIC) using redox hydrogels to produce an enzymatic biofuel cell, capable of harvesting electrical power from just a single droplet of 5 mM glucose solution...
April 26, 2017: Lab on a Chip
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