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Cell penetrating peptide

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https://www.readbyqxmd.com/read/29143522/mmp-2-sensitive-ha-end-conjugated-poly-amidoamine-dendrimers-via-click-reaction-to-enhance-drug-penetration-into-solid-tumor
#1
Min Han, Ming-Yi Huang-Fu, Wang-Wei Guo, Ningning Guo, Jiejian Chen, Huina Liu, Zhiqi Xie, Mengting Lin, Qichun Wei, Jianqing Gao
Currently, the limited tumor penetration of nanoparticles remains a major challenge of cancer nanomedicine. Herein, we propose a size-shrinkable, drug delivery system based on a polysaccharide-modified dendrimer with tumor microenvironment responsiveness for the first time to our knowledge, which was formed by conjugating the terminal glucose of hyaluronic acid (HA) to the superficial amidogen of poly(amidoamine) (PAMAM), using a matrix metalloproteinase-2 (MMP-2)-cleavable peptide (PLGLAG) via click reaction...
November 16, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29136833/a-cell-penetrating-ratiometric-probe-for-simultaneous-measurement-of-lysosomal-and-cytosolic-ph-change
#2
Meng-Chan Xia, Lesi Cai, Sichun Zhang, Xinrong Zhang
A new ratiometric fluorescent probe based on cell-penetrating peptides (CPPs) was constructed for whole-cell pH mapping and simultaneous measurement of pH changes in the cytoplasm and lysosomes. The arginine-rich CPP, R12K worked as linker, carrier and part of the fluorophore. Benefiting from R12K, the fluorescent probe is completely water soluble, membrane permeable and well biocompatible. It shows high selectivity, sensitivity and reversibility to pH fluctuations. The ratio of fluorescence intensities F519/F582 increased from 0...
February 1, 2018: Talanta
https://www.readbyqxmd.com/read/29133235/can-the-cellular-internalization-of-cargo-proteins-be-enhanced-by-fusing-a-tat-peptide-in-the-center-of-proteins-a-fluorescence-study
#3
Xiaochao Chen, Jing Chen, Rong Fu, Pingfan Rao, Richard Weller, Jeremy Brasdshaw, Shutao Liu
Aim to investigate whether the cellular uptake of cargo proteins can be enhanced by fusing a Tat peptide in the center of proteins, GST-Tat-GFP and GST-GFP-Tat proteins were firstly constructed and expressed. The cellular internalization of both proteins was then evaluated and compared in HeLa cells by using fluorescent microscopy and flow cytometry, as well as the transdermal delivery in human skin by using confocal microscopy. Results from in-vitro cell experiments showed that GST-Tat-GFP protein efficiently internalized into HeLa cells when a Tat peptide was fused in the center of proteins, whereas its efficiency is lower than that of GST-GFP-Tat protein with a Tat peptide terminal fused...
November 10, 2017: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29133119/towards-topical-microrna-directed-therapy-for-epidermal-disorders
#4
REVIEW
Kehinde Ross
There remains an unmet need dermatological need for innovative topical agents that achieve better long term outcomes with fewer side effects. Modulation of the expression and activity of microRNA (miRNAs) represents an emerging translational framework for the development of such innovative therapies because changes in the expression of one miRNA can have wide-ranging effects on diverse cellular processes associated with disease. In this short review, the roles of miRNA in epidermal development, psoriasis, cutaneous squamous cell carcinoma and re-epithelisation are highlighted...
November 10, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/29125836/a-simple-in-vitro-tumor-chemosensitivity-assay-based-on-cell-penetrating-peptide-tagged-luciferase
#5
Tingyu Yu, Jiao Lin, Jin Zhao, Wei Huang, Lingwen Zeng, Zhiyuan Fang, Ning Xu
The analysis of intracellular ATP can reveal the response of cells to different treatments and is important for individualized medicine. In the present study, we developed a cell penetrating peptides (CPPs) tagged luciferase (TAT-LUC) for tumor chemosensitivity assay. The activity of recombinant TAT-LUC was evaluated using ATP standard solution and tumor cells. This recombinant TAT-LUC was then used for the analysis of sensitivity index (SI) of four strains of tumor cells. The results showed that TAT-LUC could detect less than 10 nM extracellular ATP with a strong correlation between the luminescence intensity and the ATP content (R2 = 0...
2017: PloS One
https://www.readbyqxmd.com/read/29125066/autonomous-self-navigating-drug-delivery-vehicles-from-science-fiction-to-reality
#6
Valery A Petrenko
Low efficacy of targeted nanomedicines in biological experiments enforced us to challenge the traditional concept of drug targeting and suggest a paradigm of 'addressed self-navigating drug-delivery vehicles,' in which affinity selection of targeting peptides and vasculature-directed in vivo phage screening is replaced by the migration selection, which explores ability of 'promiscuous' phages and their proteins to migrate through the tumor-surrounding cellular barriers, using a 'hub and spoke' delivery strategy, and penetrate into the tumor affecting the diverse tumor cell population...
December 2017: Therapeutic Delivery
https://www.readbyqxmd.com/read/29123382/in-vivo-study-of-doxorubicin-loaded-cell-penetrating-peptide-modified-ph-sensitive-liposomes-biocompatibility-bio-distribution-and-pharmacodynamics-in-balb-c-nude-mice-bearing-human-breast-tumors
#7
Yuan Ding, Wei Cui, Dan Sun, Gui-Ling Wang, Yu Hei, Shuai Meng, Jian-Hua Chen, Ying Xie, Zhi-Qiang Wang
In vivo evaluation of drug delivery vectors is essential for clinical translation. In BALB/c nude mice bearing human breast cancer tumors, we investigated the biocompatibility, pharmacokinetics, and pharmacodynamics of doxorubicin (DOX)-loaded novel cell-penetrating peptide (CPP)-modified pH-sensitive liposomes (CPPL) (referred to as CPPL(DOX)) with an optimal CPP density of 4%. In CPPL, a polyethylene glycol (PEG) derivative formed by conjugating PEG with stearate via acid-degradable hydrazone bond (PEG2000-Hz-stearate) was inserted into the surface of liposomes, and CPP was directly attached to liposome surfaces via coupling with stearate to simultaneously achieve long circulation time in blood and improve the selectivity and efficacy of CPP for tumor targeting...
2017: Drug Design, Development and Therapy
https://www.readbyqxmd.com/read/29117144/cell-penetrating-peptides-design-strategies-beyond-primary-structure-and-amphipathicity
#8
REVIEW
Daniela Kalafatovic, Ernest Giralt
Efficient intracellular drug delivery and target specificity are often hampered by the presence of biological barriers. Thus, compounds that efficiently cross cell membranes are the key to improving the therapeutic value and on-target specificity of non-permeable drugs. The discovery of cell-penetrating peptides (CPPs) and the early design approaches through mimicking the natural penetration domains used by viruses have led to greater efficiency of intracellular delivery. Following these nature-inspired examples, a number of rationally designed CPPs has been developed...
November 8, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29115187/hyaluronate-parathyroid-hormone-peptide-conjugate-for-transdermal-treatment-of-osteoporosis
#9
Minsoo Cho, Seulgi Han, Hyemin Kim, Ki Su Kim, Sei Kwang Hahn
Human parathyroid hormone 1-34 fragment (PTH1-34) has been used as a FDA-approved therapeutics to treat osteoporosis by daily subcutaneous injection. In this work, we successfully developed PTH1-34 conjugated hyaluronic acid (HA) for the transdermal treatment of osteoporosis with improved patient compliance. HA-PTH1-34 conjugate was synthesized by the coupling reaction between aldehyde group introduced to HA and amine group of PTH1-34. After characterization by gel permeation chromatography (GPC) and ELISA, the biological effect of HA-PTH1-34 conjugate on the proliferation of human osteoblast cells was confirmed by in vitro calcium colorimetric assay and cAMP assay...
November 8, 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/29113134/non-covalent-loading-of-anti-cancer-doxorubicin-by-modularizable-peptide-self-assemblies-for-a-nanoscale-drug-carrier
#10
Kin-Ya Tomizaki, Kohei Kishioka, Shunsuke Kataoka, Makoto Miyatani, Takuya Ikeda, Mami Komada, Takahito Imai, Kenji Usui
We prepared nanoscale, modularizable, self-assembled peptide nanoarchitectures with diameters less of than 20 nm by combining β-sheet-forming peptides tethering a cell-penetrating peptide or a nuclear localization signal sequence. We also found that doxorubicin (Dox), an anti-cancer drug, was non-covalently accommodated by the assemblies at a ratio of one Dox molecule per ten peptides. The Dox-loaded peptide assemblies facilitated cellular uptake and subsequent nuclear localization in HeLa cells, and induced cell death even at low Dox concentrations...
November 6, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29109776/sequential-delivery-of-cyclopeptide-ra-v-and-doxorubicin-for-combination-therapy-on-resistant-tumor-and-in-situ-monitoring-of-cytochrome-c-release
#11
Huachao Chen, Yurong Wang, Yongrong Yao, Shenglin Qiao, Hao Wang, Ninghua Tan
A programmed drug delivery system that can achieve sequential release of multiple therapeutics under different stimulus holds great promise to enhance the treatment efficacy and overcome multi-drug resistance (MDR) in tumor. Herein, multi-organelle-targeted and pH/ cytochrome c (Cyt c) dual-responsive nanoparticles were designed for combination therapy on resistant tumor. In this system (designated DGLipo NPs), doxorubicin (Dox) was intercalated into the DNA duplex containing a Cyt c aptamer, which subsequently loaded in the dendrigraftpoly-L-lysines (DGL) cores of DGLipo NPs, while cyclopeptide RA-V was doped into the pH-sensitive liposomal shells...
2017: Theranostics
https://www.readbyqxmd.com/read/29108892/antifungal-activity-of-latarcin-1-derived-cell-penetrating-peptides-against-fusarium-solani
#12
Deepthi Poornima Budagavi, Sheeba Zarin, Archana Chugh
Cell-penetrating peptides and antimicrobial peptides share physicochemical characteristics and mechanisms of interaction with biological membranes, hence, termed as membrane active peptides. The present study aims at evaluating AMP activity of CPPs. LDP-NLS and LDP are Latarcin 1 derived cell-penetrating peptides and in the current study we have evaluated antifungal and cell-penetrating properties of these CPPs in Fusarium solani. We observed that LDP-NLS and LDP exhibited excellent antifungal activity against the fungus...
November 3, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29100129/liposomes-containing-cholesterol-and-mitochondria-penetrating-peptide-mpp-for-targeted-delivery-of-antimycin-a-to-a549-cells
#13
Sudipta Mallick, Le Thi Thuy, Seulgi Lee, Jong-Ii Park, Joon Sig Choi
No abstract text is available yet for this article.
October 18, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29095595/matrix-metalloproteinase-cleavable-nanoparticles-for-tumor-microenvironment-and-tumor-cell-dual-targeting-drug-delivery
#14
Zhenliang Sun, Ruihong Li, Ji Sun, You Peng, Linlin Xiao, Xingxing Zhang, Yixin Xu, Man Wang
Matrix metalloproteinases (MMPs), mostly abundant in the tumor extracellular matrix (ECM), tumor cells, and tumor vasculatures, are closely correlated with tumor progression and metastasis. In this case, making use of MMPs was supposed to achieve site-specific drug delivery and a satisfactory tumor treatment effect. Herein, we rationally developed a novel tumor microenvironment and tumor cell dual-targeting nanoparticle by integrating a chemotherapeutic-loaded drug-loaded carrier and a versatile polypeptide-LinTT1-PVGLIG-TAT (LPT) which is composed of a multitargeting peptide-LinTT1 and a cell-penetrating peptide-TAT...
November 9, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29094037/solid-phase-synthesis-of-difficult-purine-rich-pnas-through-selective-hmb-incorporation-application-to-the-total-synthesis-of-cell-penetrating-peptide-pnas
#15
Julien Tailhades, Hotake Takizawa, Michael J Gait, Don A Wellings, John D Wade, Yoshitsugu Aoki, Fazel Shabanpoor
Antisense oligonucleotide (ASO)-based drug development is gaining significant momentum following the recent FDA approval of Eteplirsen (an ASO based on phosphorodiamidate morpholino) and Spinraza (2'-O-methoxyethyl-phosphorothioate) in late 2016. Their attractiveness is mainly due to the backbone modifications which have improved the in vivo characteristics of oligonucleotide drugs. Another class of ASO, based on peptide nucleic acid (PNA) chemistry, is also gaining popularity as a platform for development of gene-specific therapy for various disorders...
2017: Frontiers in Chemistry
https://www.readbyqxmd.com/read/29090725/arginine-modification-of-lycosin-i-to-improve-inhibitory-activity-against-cancer-cells
#16
Peng Zhang, Jing Ma, Yujie Yan, Bo Chen, Bobo Liu, Cui Jian, Baode Zhu, Songping Liang, Youlin Zeng, Zhonghua Liu
Lycosin-I is a linear amphipathic α-helical anticancer peptide (ACP) extracted from the spider Lycosa singoriensis, which can activate the mitochondrial death pathway to induce apoptosis in tumor cells and up-regulate p27 to inhibit cell proliferation. However, the applicability of lycosin-I as a novel anticancer drug is limited by its low cellular entry and efficacy in solid tumors. Amino acid substitution presents an effective and modest strategy to improve the anticancer activity and bioavailability of ACPs...
November 15, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/29090413/enhanced-transdermal-delivery-with-less-irritation-by-magainin-pore-forming-peptide-with-a-n-lauroylsarcosine-and-sorbitan-monolaurate-mixture
#17
Haerin Lee, Juhyun Park, Yeu-Chun Kim
Transdermal drug delivery is advantageous over other conventional drug administration routes. However, it can be inefficient because of the natural barrier of the stratum corneum which is the uppermost layer of the skin. A previous study verified that the treatment of magainin pore-forming peptide with N-lauroylsarcosine (NLS) on human skin can increase skin permeability by 47-fold. However, NLS is well known as a potential skin irritant. The irritation potential of NLS is known to decrease when mixed with sorbitan monolaurate (S20)...
October 31, 2017: Drug Delivery and Translational Research
https://www.readbyqxmd.com/read/29081059/the-current-role-of-cell-penetrating-peptides-in-cancer-therapy
#18
Lucia Feni, Ines Neundorf
Cell-penetrating peptides (CPPs) are a heterogeneous class of peptides with the ability to translocate across the plasma membrane and to carry attached cargos inside the cell. Two main entry pathways are discussed, as direct translocation and endocytosis , whereas the latter is often favored when bulky cargos are added to the CPP. Attachment to the CPP can be achieved by means of covalent coupling or non-covalent complex formation, depending on the chemical nature of the cargo. Owing to their striking abilities the further development and application of CPP-based delivery strategies has steadily emerged during the past years...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29081058/empowering-the-potential-of-cell-penetrating-peptides-for-targeted-intracellular-delivery-via-molecular-self-assembly
#19
Yejiao Shi, João Conde, Helena S Azevedo
Cell-penetrating peptides (CPPs) have been widely explored as an effective tool to deliver a variety of molecules and nanoparticles into cells due to their intrinsic property to translocate across cell membranes. CPPs are easier to synthesize and functionalize, and their incorporation into delivery vehicles could be achieved by both non-covalent and covalent methods. Recent advances in molecular self-assembly have demonstrated the possibility to fabricate various nanostructures with precise control over the shape, size and presentation of diverse functionalities...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29081057/uptake-mechanism-of-cell-penetrating-peptides
#20
Maxime Gestin, Moataz Dowaidar, Ülo Langel
Cell-penetrating peptides have been extensively used since their discovery for delivering cargoes unable to cross the cell membrane. Among other transported cargoes, they have shown very efficient delivery for oligonucleotides making cell-penetrating peptides a powerful tool for gene therapy. Numerous cell-penetrating peptides have now been discovered offering a wide library of structures and mechanisms of actions. Nevertheless, if it is known that different pathways are available for particles to be taken up, most mechanisms by which these particles enter cells are still to be characterized more precisely...
2017: Advances in Experimental Medicine and Biology
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