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Co enzyme q 10

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https://www.readbyqxmd.com/read/27666706/-experimental-study-on-substance-p-in-the-regulation-of-degranulation-of-cultured-murine-mast-cells
#1
F L Cheng, Q T Li, C Q Zhao, Y F An, X P Qi
Objective: To investigate the effect of interleukin-4 (IL-4) stimulation on the expression of FcεRⅠα and NK-1R on mature mast cells(MC) cultured and differentiated from mouse bone marrow stem cells, and then to study if these MC also respond to substance P (SP) both in FcεRⅠα and NK-1R dependent manners. Methods: Bone marrow cells were aseptically flushed from BALB/c mouse femurs into complete RPMI 1640, followed by culture with stem cell factor (SCF 100 μg/L), IL-3 (15 μg/L) and IL-4 (0, 10, 15, 20 and 25 μg/L, respectively)...
September 7, 2016: Zhonghua Er Bi Yan Hou Tou Jing Wai Ke za Zhi, Chinese Journal of Otorhinolaryngology Head and Neck Surgery
https://www.readbyqxmd.com/read/27593931/methyl-cpg-binding-protein-mbd2-plays-a-key-role-in-maintenance-and-spread-of-dna-methylation-at-cpg-islands-and-shores-in-cancer
#2
C Stirzaker, J Z Song, W Ng, Q Du, N J Armstrong, W J Locke, A L Statham, H French, R Pidsley, F Valdes-Mora, E Zotenko, S J Clark
Cancer is characterised by DNA hypermethylation and gene silencing of CpG island-associated promoters, including tumour-suppressor genes. The methyl-CpG-binding domain (MBD) family of proteins bind to methylated DNA and can aid in the mediation of gene silencing through interaction with histone deacetylases and histone methyltransferases. However, the mechanisms responsible for eliciting CpG island hypermethylation in cancer, and the potential role that MBD proteins play in modulation of the methylome remain unclear...
September 5, 2016: Oncogene
https://www.readbyqxmd.com/read/27412900/monosialoganglioside-containing-nanoliposomes-restore-endothelial-function-impaired-by-al-amyloidosis-light-chain-proteins
#3
Daniel A Franco, Seth Truran, Volkmar Weissig, Diana Guzman-Villanueva, Nina Karamanova, Subhadip Senapati, Camelia Burciu, Marina Ramirez-Alvarado, Luis M Blancas-Mejia, Stuart Lindsay, Parameswaran Hari, Raymond Q Migrino
BACKGROUND: Light chain amyloidosis (AL) is associated with high mortality, especially in patients with advanced cardiovascular involvement. It is caused by toxicity of misfolded light chain proteins (LC) in vascular, cardiac, and other tissues. There is no treatment to reverse LC tissue toxicity. We tested the hypothesis that nanoliposomes composed of monosialoganglioside, phosphatidylcholine, and cholesterol (GM1 ganglioside-containing nanoliposomes [NLGM1]) can protect against LC-induced human microvascular dysfunction and assess mechanisms behind the protective effect...
June 2016: Journal of the American Heart Association
https://www.readbyqxmd.com/read/27112133/cloning-purification-and-characterization-of-xylose-isomerase-from-thermotoga-naphthophila-rku-10
#4
Bilqees Fatima, Muhammad Nauman Aftab, Ikram-Ul Haq
A 1.3 kb xyl-A gene encoding xylose isomerase from a hyperthermophilic eubacterium Thermotoga naphthophila RKU-10 (TnapXI) was cloned and over-expressed in Escherichia coli to produce the enzyme in mesophilic conditions that work at high temperature. The enzyme was concentrated by lyophilization and purified by heat treatment, fractional precipitation, and UNOsphere Q anion-exchange column chromatography to homogeneity level. The apparent molecular mass was estimated by SDS-PAGE to be 49.5 kDa. The active enzyme showed a clear zone on Native-PAGE when stained with 2, 3, 5-triphenyltetrazolium chloride...
September 2016: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/26927445/sodium-glucose-co-transporter-2-inhibitors-suppress-atrial-natriuretic-peptide-secretion-in-patients-with-newly-diagnosed-type-2-diabetes
#5
Y Wang, L Xu, L Yuan, D Li, Y Zhang, R Zheng, C Liu, X Feng, Q Li, Q Li, J Ma
AIMS: To observe changes in atrial natriuretic peptide levels after treatment with sodium-glucose co-transporter-2 inhibitors in patients with newly diagnosed type 2 diabetes. METHODS: A total of 28 patients with newly diagnosed Type 2 diabetes and HbA1c levels of 58 -91 mmol/mol (7.5-10.5%) were randomly selected to receive sodium-glucose co-transporter-2 inhibitor treatment (n = 18) or placebo (n = 10) for 24 weeks. We analysed atrial natriuretic peptide concentrations, using an enzyme-linked immunosorbent assay...
December 2016: Diabetic Medicine: a Journal of the British Diabetic Association
https://www.readbyqxmd.com/read/25783971/evidence-of-a-role-for-gtp-cyclohydrolase-1-in-visceral-pain
#6
D C Bulmer, C A Botha, A Wheeldon, K Grey, C A Mein, K Lee, C H Knowles, W J Winchester, Q Aziz
BACKGROUND: The enzyme guanosine triphosphate-cyclohydrolase-1 (GCH-1) is a rate limiting step in the de novo synthesis of tetrahydrobiopterin (BH4) a co-factor in monoamine synthesis and nitric oxide production. GCH-1 is strongly implicated in chronic pain based on data generated using the selective GCH-1 inhibitor 2,4-diamino-6-hydroxypyrimidine (DAHP), and studies which have identified a pain protective GCH-1 haplotype associated with lower BH4 production and reduced pain. METHODS: To investigate the role for GCH-1 in visceral pain we examined the effects of DAHP on pain behaviors elicited by colorectal injection of mustard oil in rats, and the pain protective GCH-1 haplotype in healthy volunteers characterized by esophageal pain sensitivity before and after acid injury, and assessed using depression and anxiety questionnaires...
May 2015: Neurogastroenterology and Motility: the Official Journal of the European Gastrointestinal Motility Society
https://www.readbyqxmd.com/read/25783038/metabolic-phenotype-microrna-data-fusion-analysis-of-the-systemic-consequences-of-roux-en-y-gastric-bypass-surgery
#7
Q Wu, J V Li, F Seyfried, C W le Roux, H Ashrafian, T Athanasiou, W Fenske, A Darzi, J K Nicholson, E Holmes, N J Gooderham
BACKGROUND/OBJECTIVES: Bariatric surgery offers sustained marked weight loss and often remission of type 2 diabetes, yet the mechanisms of establishment of these health benefits are not clear. SUBJECTS/METHODS: We mapped the coordinated systemic responses of gut hormones, the circulating miRNAome and the metabolome in a rat model of Roux-en-Y gastric bypass (RYGB) surgery. RESULTS: The response of circulating microRNAs (miRNAs) to RYGB was striking and selective...
July 2015: International Journal of Obesity: Journal of the International Association for the Study of Obesity
https://www.readbyqxmd.com/read/25510509/cortical-fast-spiking-parvalbumin-interneurons-enwrapped-in-the-perineuronal-net-express-the-metallopeptidases-adamts8-adamts15-and-neprilysin
#8
J Rossier, A Bernard, J-H Cabungcal, Q Perrenoud, A Savoye, T Gallopin, M Hawrylycz, M Cuénod, K Do, A Urban, Ed S Lein
The in situ hybridization Allen Mouse Brain Atlas was mined for proteases expressed in the somatosensory cerebral cortex. Among the 480 genes coding for protease/peptidases, only four were found enriched in cortical interneurons: Reln coding for reelin; Adamts8 and Adamts15 belonging to the class of metzincin proteases involved in reshaping the perineuronal net (PNN) and Mme encoding for Neprilysin, the enzyme degrading amyloid β-peptides. The pattern of expression of metalloproteases (MPs) was analyzed by single-cell reverse transcriptase multiplex PCR after patch clamp and was compared with the expression of 10 canonical interneurons markers and 12 additional genes from the Allen Atlas...
February 2015: Molecular Psychiatry
https://www.readbyqxmd.com/read/25273816/gene-cloning-and-enzymatic-characterization-of-alkali-tolerant-type-i-pullulanase-from-exiguobacterium-acetylicum
#9
Y Qiao, Q Peng, J Yan, H Wang, H Ding, B Shi
UNLABELLED: A pullulanase gene (Pul3YH5) of 2568 bp, which encodes a protein containing 855 amino acid residues, was cloned from the alkaliphilic bacterium Exiguobacterium acetylicum YH5. The pullulanase (Pul3YH5) contains the YNWGYDP motif of type I pullulanase as well as four conserved glycoside hydrolase sequences of the GH13 (α-amylase) family. When the pullulanase gene was cloned and expressed in Escherichia coli BL21 (DE3) plysS, the recombinant pullulanase had a molecular mass of ˜100·0 kDa...
January 2015: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/24690287/purification-and-characterization-of-%C3%AE-amylase-from-miswak-salvadora-persica
#10
Saleh A Mohamed, Yaaser Q Almulaiky, Youssri M Ahmed, Omar A M Al-Bar, Ibrahim H Ibrahim
BACKGROUND: The miswak (Salvadora persica) is a natural toothbrush. It is well known that very little information has been reported on enzymes in miswak as medicinal plant. Recently, we study peroxidase in miswak. In the present study, the main goal of this work is to purify and characterize α-amylase from miswak. The second goal is to study the storage stability of α-amylase in toothpaste. METHOD: The purification method included chromatography of miswak α-amylase on DEAE-Sepharose column and Sephacryl S-200 column...
2014: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/24489336/a8-31-characterisation-of-human-transitional-b-cells-though-their-regulatory-functions
#11
S Hillion, Q Simon, C Jamin, Y Renaudineau, J O Pers
Transitional B cells represent a cortical link between the initial B cell compartment in bone marrow and the mature peripheral B cell repertoire. The prior characterisation of human transitional B-cells has suggested that this population could be subdivised into two major subsets: the immature T1-Bcells CD19(+) CD10high CD24high CD38high IgMhigh IgDlow CD21low and tne intermediate cells mentionned as T2-B cells CD19(+) CD10 + CD24high CD38high IgMhigh IgDhigh CD21high. Recently B-cell regulation have been shed in light and a lot of evidences suggested that regulatory B cells (Bregs) are contained within the CD24high CD38high transitional B-cell subsets...
March 1, 2014: Annals of the Rheumatic Diseases
https://www.readbyqxmd.com/read/24426207/hematological-parameters-and-rbc-tbars-level-of-q-10-supplemented-tribal-sickle-cell-patients-a-hospital-based-study
#12
A S Thakur, G P Littaru, S Moesgaard, C Dan Sindberg, Y Khan, C M Singh
The study has been undertaken as number of sickle cell patients in Chhattisgarh tribal population is 23.7 %. The Co enzyme Q10 is a strong antioxidant and energy producing compound. The patients were divided into three groups group A homozygous (SS), group B heterozygous (AS) and group C controls for TBARS study. The age group is 10-55 years and 200 mg of CoQ10 was given to A and B groups. The hematological parameters, C reactive protein as well as RBC TBARS level were performed by usual and standard techniques...
April 2013: Indian Journal of Clinical Biochemistry: IJCB
https://www.readbyqxmd.com/read/24313872/co-culture-of-spleen-stromal-cells-with-bone-marrow-mononuclear-cells-leads-to-the-generation-of-a-novel-macrophage-subset
#13
X Wang, Y Li, H Xiao, X Zhang, J Cao, D Zhang, J Zhang, X Li, B Shen, Q Wang, Y Shi
Macrophages adopt diverse activation states depending on the microenvironment. Recently, stromal cells have been demonstrated to be organizers of the microenvironment. Here, using splenic stromal cells to mimic the splenic microenvironment in vivo, we show that spleen stromal cells can programme bone marrow-derived mononuclear cells to differentiate and polarize into a novel macrophage subset. These differentiated macrophages (Diff-Mφ) exhibited pronounced production of IL-10, IL-6 and TNF-α, but diminished the production of IL-12 in response to LPS...
January 2014: Scandinavian Journal of Immunology
https://www.readbyqxmd.com/read/23945350/co-infection-of-human-parvovirus-b19-with-plasmodium-falciparum-contributes-to-malaria-disease-severity-in-gabonese-patients
#14
Nguyen L Toan, Bui T Sy, Le H Song, Hoang V Luong, Nguyen T Binh, Vu Q Binh, Reinhard Kandolf, Thirumalaisamy P Velavan, Peter G Kremsner, C-Thomas Bock
BACKGROUND: High seroprevalence of parvovirus B19 (B19V) coinfection with Plasmodium falciparum has been previously reported. However, the impact of B19V-infection on the clinical course of malaria is still elusive. In this study, we investigated the prevalence and clinical significance of B19V co-infection in Gabonese children with malaria. METHODS: B19V prevalence was analyzed in serum samples of 197 Gabonese children with P. falciparum malaria and 85 healthy controls using polymerase chain reaction (PCR), enzyme-linked immunosorbent assay (ELISA), and direct DNA-sequencing...
2013: BMC Infectious Diseases
https://www.readbyqxmd.com/read/23832859/ligase-independent-cloning-of-amylase-gene-from-a-local-bacillus-subtilis-isolate-and-biochemical-characterization-of-the-purified-enzyme
#15
Merve Tuzlakoglu Ozturk, Nagihan Akbulut, Saliha Issever Ozturk, Fusun Gumusel
Five hundred ninety-seven bacterial isolates from Turkish hot spring water sources were screened for their ability to produce extracellular α-amylase. Among them, a high enzyme-producing Bacillus subtilis isolate, A28, was selected, and its α-amylase gene was cloned and expressed in Escherichia coli by a ligase-independent method. α-Amylase from the recombinant strain was purified to homogeneity by Q-Sepharose anion exchange and Sephacryl S-100 gel filtration chromatographies. The final yield of the enzyme was about 22...
September 2013: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/23726951/biochemical-and-molecular-characterization-of-pseudomonas-aeruginosa-ctm50182-organic-solvent-stable-elastase
#16
Bassem Jaouadi, Nadia Zaraî Jaouadi, Hatem Rekik, Belgacem Naili, Abdelhamid Beji, Abdelhafidh Dhouib, Samir Bejar
An extracellular alkaline elastase was produced from Pseudomonas aeruginosa CTM50182. It was chromatographically purified using HPLC and Mono Q Sepharose column. Matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF/MS) analysis revealed that the purified enzyme (called AMPP) was a monomer with a molecular mass of 33,015.18 Da. The N-terminal 29 amino acid sequence of AMPP showed high homology with those of Pseudomonas elastases. It showed optimal activity at pH 12 and 80 °C and was stable at a pH range of 9-12 after 120 h of incubation...
September 2013: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/23322529/purification-and-characterization-of-a-novel-acid-phosphatase-from-the-split-gill-mushroom-schizophyllum-commune
#17
Guo-Qing Zhang, Qing-Jun Chen, Jian Sun, He-Xiang Wang, Chun-Hua Han
A monomeric acid phosphatase (ACP) with a molecular mass of 72.5 kDa was purified from fresh fruiting bodies of cultured Schizophyllum commune mushroom. The isolation procedure entailed ion exchange chromatography on DEAE-cellulose, CM-cellulose, and Q-sepharose, and gel filtration by fast protein liquid chromatography on Superdex 75. It demonstrated a unique N-terminal amino acid sequence of NAPWAQIDEV, which exhibited 60% amino acid identity to that of S. commune hypothetical histidine ACP based on its genome sequence, but less than 30% amino acid identity to that of other fungal ACPs previously reported...
October 2013: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/23076940/antioxidants-for-chronic-kidney-disease
#18
REVIEW
Min Jun, Vinod Venkataraman, Mona Razavian, Bruce Cooper, Sophia Zoungas, Toshiharu Ninomiya, Angela C Webster, Vlado Perkovic
BACKGROUND: Chronic kidney disease (CKD) is a significant risk factor for premature cardiovascular disease and death. Increased oxidative stress in people with CKD has been implicated as a potential causative factor for some cardiovascular diseases. Antioxidant therapy may reduce cardiovascular mortality and morbidity in people with CKD. OBJECTIVES: To examine the benefits and harms of antioxidant therapy on mortality and cardiovascular events in people with CKD stages 3 to 5; dialysis, and kidney transplantation patients...
2012: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/23002221/fur-activates-expression-of-the-2-oxoglutarate-oxidoreductase-genes-oordabc-in-helicobacter-pylori
#19
Jeremy J Gilbreath, Abby L West, Oscar Q Pich, Beth M Carpenter, Sarah Michel, D Scott Merrell
Helicobacter pylori is a highly successful pathogen that colonizes the gastric mucosa of ∼50% of the world's population. Within this colonization niche, the bacteria encounter large fluctuations in nutrient availability. As such, it is critical that this organism regulate expression of key metabolic enzymes so that they are present when environmental conditions are optimal for growth. One such enzyme is the 2-oxoglutarate (α-ketoglutarate) oxidoreductase (OOR), which catalyzes the conversion of α-ketoglutarate to succinyl coenzyme A (succinyl-CoA) and CO(2)...
December 2012: Journal of Bacteriology
https://www.readbyqxmd.com/read/22912870/mitochondria-derived-superoxide-links-to-tourniquet-induced-apoptosis-in-mouse-skeletal-muscle
#20
Thai P Tran, Huiyin Tu, Jinxu Liu, Robert L Muelleman, Yu-Long Li
Our previous study has reported that superoxide mediates ischemia-reperfusion (IR)-induced necrosis in mouse skeletal muscle. However, it remains poorly understood whether IR induces apoptosis and what factors are involved in IR-induced apoptosis in skeletal muscle. Using a murine model of tourniquet-induced hindlimb IR, we investigated the relationship between mitochondrial dysfunction and apoptosis in skeletal muscle. Hindlimbs of C57/BL6 mice were subjected to 3 h ischemia and 4 h reperfusion via placement and release of a rubber tourniquet at the greater trochanter...
2012: PloS One
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