keyword
https://read.qxmd.com/read/38333929/hydrogen-bioelectrogeneration-with-ph-resilient-and-oxygen-tolerant-cobalt-apoenzyme-saccharide
#1
JOURNAL ARTICLE
Rodrigo M Iost, Radhakrishnan Venkatkarthick, Steffane Q Nascimento, Fabio H B Lima, Frank N Crespilho
Hydrogenases are enzymes that catalyze the reversible conversion of protons to hydrogen gas, using earth-abundant metals such as nickel and/or iron. This characteristic makes them promising for sustainable energy applications, particularly in clean hydrogen production. However, their widespread use faces challenges, including a limited pH range and susceptibility to oxygen. In response to these issues, SacCoMyo is introduced as an artificial enzyme. SacCoMyo is designed by replacing the native metal in the myoglobin (Myo) scaffold with a hydroxocobalamin (Co) porphyrin core and complemented by a protective heteropolysaccharide-linked (Sac) shell...
February 9, 2024: Chemical Communications: Chem Comm
https://read.qxmd.com/read/38279104/decoction-regulating-phytochemicals-micromorphology-changes-and-anti-inflammation-activity-enhancements-originated-from-herb-medicine-supermolecules
#2
JOURNAL ARTICLE
Luping Yang, Xiang Zhang, Zhijia Wang, Xiaoyu Lin, Yaozhi Zhang, Jihui Lu, Linying Wu, Shuchang Yao, Wenguang Jing, Xuemei Huang, Penglong Wang
BACKGROUND: Mahuang Fuzi decoction (MGF) is composed of three herb medicines that has been clinically used to treat inflammatory diseases for a long history. At present, more and more active phytochemicals' aggregations have been found during the thermodynamic process of herb medicine decoction, and revealing the clinical efficacy of herb medicine through supramolecular strategies is the focus of current research. However, it is not clear whether decoction induced supermolecules' morphological changes to modify activity...
January 26, 2024: Chinese Medicine
https://read.qxmd.com/read/38234747/s1-hydrophobic-residues-alter-the-voltage-sensing-phosphatase-enzymatic-function-and-voltage-sensing
#3
Vamseedhar Rayaprolu, Heini M Miettinen, William Baker, Matthew Fisher, Gwendolyn Mueller, William O Rankin, J Jack Kelly, William J Ratzan, Lee Min Leong, Joshua A Davisson, Bradley J Baker, Susy C Kohout
The voltage sensing domain (VSD) is a four-helix modular protein domain that converts electrical signals into conformational changes, leading to open pores and active enzymes. In most voltage sensing proteins, the VSDs do not interact with one another and the S1-S3 helices are considered mainly as scaffolding. The two exceptions are the voltage sensing phosphatase (VSP) and the proton channel (Hv). VSP is a voltage-regulated enzyme and Hv's are channels that only have VSDs. To investigate the S1 contribution to VSP function, we individually mutated four hydrophobic amino acids in S1 to alanine (F127, I131, I134 and L137)...
December 27, 2023: bioRxiv
https://read.qxmd.com/read/38035520/mitigative-potential-of-kaempferide-against-polyethylene-microplastics-induced-testicular-damage-by-activating-nrf-2-keap-1-pathway
#4
JOURNAL ARTICLE
Muhammad Umar Ijaz, Zainab Rafi, Ali Hamza, Amany A Sayed, Ghadeer M Albadrani, Muath Q Al-Ghadi, Mohamed M Abdel-Daim
Polyethylene microplastics (PE-MPs) are one of the environmental contaminants that instigate oxidative stress (OS) in various organs of the body, including testes. Kaempferide (KFD) is a plant-derived natural flavonol with potential neuroprotective, hepatoprotective, anti-cancer, anti-oxidant and anti-inflammatory properties. Therefore, the present study was designed to evaluate the alleviative effects of KFD against PE-MPs-prompted testicular toxicity in rats. Fourty eight adult male albino rats were randomly distributed into 4 groups: control, PE-MPs-administered (1...
November 28, 2023: Ecotoxicology and Environmental Safety
https://read.qxmd.com/read/37968076/-lactate-promotes-hmgb1-phosphorylation-and-release-via-akt-signaling-pathway-in-gastric-cancer-cells-hgc-27
#5
JOURNAL ARTICLE
X L Chen, F Ge, M Q Wan, S M Qi, Z L Qi
Objective: To investigate the molecular mechanism of how lactate induces high mobility group box 1 (HMGB1) release. Methods: Gastric cancer HGC-27 cells were divided into the control group and the lactate group (The cells were treated with lactate for 6 h). The level of HMGB1 in the cell culture medium was detected by enzyme-linked immunosorbent assay (ELISA), the localization of HMGB1 was detected using laser confocal microscopy, and the nuclear translocation of HMGB1 was detected using the nucleoplasmic separation assay...
November 23, 2023: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
https://read.qxmd.com/read/37961685/queuosine-biosynthetic-enzyme-quee-moonlights-as-a-cell-division-regulator
#6
Samuel A Adeleye, Srujana S Yadavalli
In many organisms, stress responses to adverse environments can trigger secondary functions of certain proteins by altering protein levels, localization, activity, or interaction partners. Escherichia coli cells respond to the presence of specific cationic antimicrobial peptides by strongly activating the PhoQ/PhoP two-component signaling system, which regulates genes important for growth under this stress. As part of this pathway, a biosynthetic enzyme called QueE, which catalyzes a step in the formation of queuosine (Q) tRNA modification is upregulated...
November 1, 2023: bioRxiv
https://read.qxmd.com/read/37861415/characterizing-the-biosynthesis-of-the-fe-ii-cn-co-2-cysteinate-organometallic-product-of-the-radical-sam-enzyme-hydg-by-epr-and-m%C3%A3-ssbauer-spectroscopy
#7
JOURNAL ARTICLE
David G Villarreal, Guodong Rao, Lizhi Tao, Liang Liu, Thomas B Rauchfuss, R David Britt
[FeFe]-hydrogenases employ a catalytic H-cluster, consisting of a [4Fe-4S]H cluster linked to a [2Fe]H subcluster with CO, CN- ligands, and an azadithiolate bridge, which mediates the rapid redox interconversion of H+ and H2 . In the biosynthesis of this H-cluster active site, the radical S -adenosyl-l-methionine (radical SAM, RS) enzyme HydG plays the crucial role of generating an organometallic [Fe(II)(CN)(CO)2 (cysteinate)]- product that is en route to forming the H-cluster. Here, we report direct observation of this diamagnetic organometallic Fe(II) complex through Mössbauer spectroscopy, revealing an isomer shift of δ = 0...
October 20, 2023: Journal of Physical Chemistry. B
https://read.qxmd.com/read/37693483/the-mechanism-of-g%C3%AE-q-regulation-of-plc%C3%AE-3-catalyzed-pip2-hydrolysis
#8
Maria E Falzone, Roderick MacKinnon
UNLABELLED: PLCβ enzymes cleave PIP2 producing IP3 and DAG. PIP2 modulates the function of many ion channels, while IP3 and DAG regulate intracellular Ca 2+ levels and protein phosphorylation by protein kinase C, respectively. PLCβ enzymes are under the control of GPCR signaling through direct interactions with G proteins Gβγ and Gα q and have been shown to be coincidence detectors for dual stimulation of Gα q and G α i coupled receptors. PLCβs are aqueous-soluble cytoplasmic enzymes, but partition onto the membrane surface to access their lipid substrate, complicating their functional and structural characterization...
August 30, 2023: bioRxiv
https://read.qxmd.com/read/37650181/-the-impact-of-human-umbilical-cord-derived-mesenchymal-stem-cells-on-the-pancreatic-function-of-type-2-diabetic-mice-and-their-regulatory-role-on-nlrp3-inflammasomes
#9
JOURNAL ARTICLE
J Wang, Y Q Yin, Y Cheng, B Li, W L Su, S Y Yu, J Xue, Y L Gu, H X Zhang, L X Zhang, L Zang, Y M Mu
Objective: To investigate the effect and regulation of umbilical cord-derived mesenchymal stem cells (UC-MSCs) on islets function and NOD-like receptor family, pyrin domain containing 3 (NLRP3) and autophagy in type 2 diabetic mellitus (T2DM) mice. Methods: Experimental study. Twenty, 8-week-old, male C57BL/6J mice were selected and divided into a normal control group ( n =5) and a high-fat feeding modeling group ( n =15). The model of T2DM was established by high-fat feeding combined with intraperitoneal injection of low-dose streptozotocin...
September 1, 2023: Zhonghua Nei Ke za Zhi [Chinese Journal of Internal Medicine]
https://read.qxmd.com/read/37531903/dctpp1-a-reliable-q-biomarker-for-comprehensive-evaluation-of-the-quality-of-tripterygium-glycoside-tablets-based-on-chemical-references
#10
JOURNAL ARTICLE
QinWei Huang, ChunMei Tan, Cheng Zheng, Hong Meng, ZhengNan Wang, Guo-Qiang Lin, WenTing Zhang, BiLian Chen, Qing-Li He
BACKGROUND: As first-line clinical drugs, tripterygium glycoside tablets (TGTs) often have inconsistent efficacy and toxic side effects, mainly due to inadequate quality control. Therefore, clinically relevant quality standards for TGTs are urgently required. PURPOSE: Based on chemical substances and considering pharmacological efficacy, we aimed to develop an effective quality evaluation method for TGTs. METHODS: Representative commercial samples of TGTs were collected from different manufacturers, and qualitative UHPLC/LTQ-Orbitrap-MS and quantitative UHPLC-MS/MS analysis methods were successfully applied to evaluate their quality similarities and differences based on their chemical properties...
July 23, 2023: Phytomedicine
https://read.qxmd.com/read/37511276/acetyl-l-carnitine-and-liposomal-co-enzyme-q-10-attenuate-hepatic-inflammation-apoptosis-and-fibrosis-induced-by-propionic-acid
#11
JOURNAL ARTICLE
Ahlam M Alhusaini, Rahaf Alsoghayer, Lina Alhushan, Abeer M Alanazi, Iman H Hasan
Propionic acid (PRA) is a metabolic end-product of enteric bacteria in the gut, and it is commonly used as a food preservative. Despite the necessity of PRA for immunity in the body, excessive exposure to this product may result in disruptive effects. The purpose of this study is to examine the hepatoprotective effects of acetyl-L-carnitine (A-CAR) and liposomal-coenzyme Q10 (L-CoQ10 ) against PRA-induced injury. Liver injury in rats was induced by oral administration of PRA, and A-CAR and L-CoQ10 were administered concurrently with PRA for 5 days...
July 15, 2023: International Journal of Molecular Sciences
https://read.qxmd.com/read/37494658/impact-of-crystallinity-on-enzyme-orientation-and-dynamics-upon-biomineralization-in-metal-organic-frameworks
#12
JOURNAL ARTICLE
Zoe Armstrong, Austin MacRae, Mary Lenertz, Qiaobin Li, Kelley Johnson, Allison Scheiwiller, Patrick Shen, Li Feng, Mohiuddin Quadir, Zhongyu Yang
Aqueous-phase co-crystallization (also known as biomimetic mineralization or biomineralization) is a unique way to encapsulate large enzymes, enzyme clusters, and enzymes with large substrates in metal-organic frameworks (MOFs), broadening the application of MOFs as enzyme carriers. The crystallinity of resultant enzyme@MOF biocomposites, however, can be low, raising a concern about how MOF crystal packing quality affects enzyme performance upon encapsulation. The challenges to overcome this concern are (1) the limited database of enzyme performance upon biomineralization in different aqueous MOFs and (2) the difficulty in probing enzyme restriction and motion in the resultant MOF scaffolds, which are related to the local crystal packing quality/density, under the interference of the MOF backgrounds...
July 26, 2023: ACS Applied Materials & Interfaces
https://read.qxmd.com/read/37480967/water-extracts-of-tibetan-medicine-wuweiganlu-attenuates-experimental-arthritis-via-inducing-macrophage-polarization-towards-the-m2-type
#13
JOURNAL ARTICLE
Yuanyuan Wen, Shijie Zhang, Xinyu Meng, Chenyang Zhao, Bao Hou, Xuexue Zhu, Weiwei Cai, Yuetao Zhou, Liying Qiu, Haijian Sun
ETHNOPHARMACOLOGICAL RELEVANCE: Wuweiganlu (WGL) is a well-known formulation described in the "Four Medical Scriptures of Tibetan medicine", which is mainly used for the treatment of Rheumatoid Arthritis (RA) and other chronic ailments prescribed by Tibetan medicine. Nonetheless, the active constituents present in the water extracts of Wuweiganlu (WGLWE) specifically targeting arthritis treatment are largely unknown. AIM OF THE STUDY: The aim of this study is to explore the effects and underlying mechanisms of the active components in WGLWE on RA...
July 20, 2023: Journal of Ethnopharmacology
https://read.qxmd.com/read/37385803/-mechanism-of-intestinal-injury-induced-by-wnt2b-high-expressed-fibroblasts-in-crohn-s-disease
#14
JOURNAL ARTICLE
Y L Cheng, S Z Xiao, D Q Liu, L L Geng, J B Gu, R Tang, L Lan, Y Zhu, P Y Chen, Z H He, S T Gong, Y Cheng
Objective: To explore the mechanism of intestinal tissue damage induced by macrophages activated by WNT2B high-expressed fibroblasts. Methods: This study involved biological information analysis, pathological tissue research and cell experimental research. The biological information of the colon tissue from the children with inflammatory bowel disease in previous study was analyzed again with single-cell sequencing. The pathological tissues were collected by colonoscopy from 10 children with Crohn's disease treated in the Department of Gastroenterology of Guangzhou Women and Children's Medical Center from July 2022 to September 2022...
July 2, 2023: Zhonghua Er Ke za Zhi. Chinese Journal of Pediatrics
https://read.qxmd.com/read/37348445/multivariate-analysis-of-enriched-landfill-soil-consortia-provide-insight-on-the-community-structural-perturbation-and-functioning-during-low-density-polyethylene-degradation
#15
JOURNAL ARTICLE
Aubrey Dickson Chigwada, Henry Joseph Oduor Ogola, Memory Tekere
Plastic-enriched sites like landfills have immense potential for discovery of microbial consortia that can efficiently degrade plastics. In this study, we used a combination of culture enrichment, high-throughput PacBio sequencing of 16 S rRNA and the ITS gene, Fourier transform infrared (FTIR), and scanning electron microscopy (SEM) to examine the compositional and diversity perturbations of bacterial and fungal consortia from landfill soils and their impact on low-density polyethylene (LDPE) film biodegradation over a 90-day period...
June 14, 2023: Microbiological Research
https://read.qxmd.com/read/37261844/discerning-of-isatin-based-monoamine-oxidase-mao-inhibitors-for-neurodegenerative-disorders-by-exploiting-2d-3d-qsar-modelling-and-molecular-dynamics-simulation
#16
JOURNAL ARTICLE
Sunil Kumar, Jayalakshmi Jayan, Amritha Manoharan, Feba Benny, Mohamed A Abdelgawad, Mohammed M Ghoneim, Mohamed El-Sherbiny, Sachithra Thazhathuveedu Sudevan, T P Aneesh, Bijo Mathew
Almost a billion people worldwide suffer from neurological disorders, which pose public health challenges. An important enzyme that is well-known for many neurodegenerative illnesses is monoamine oxidase (MAO). Although several promising drugs for the treatment of MAO inhibition have recently been examined, it is still necessary to identify the precise structural requirements for robust efficacy. Atom-based, field-based, and GA-MLR (genetic algorithm multiple linear regression) models were created for this investigation...
June 1, 2023: Journal of Biomolecular Structure & Dynamics
https://read.qxmd.com/read/37024910/an-efficient-method-for-cell-sheet-bioengineering-from-rbmscs-on-thermo-responsive-pcl-peg-pcl-copolymer
#17
JOURNAL ARTICLE
Sevil Vaghefi Moghaddam, Fatemeh Abedi, Hajie Lotfi, Roya Salehi, Abolfazl Barzegar, Mohamadreza Baghaban Eslaminejad, Mostafa Khalili, Effat Alizadeh
Utilizing both medium enrichment and a thermos-responsive substrate to maintain the cell-to-cell junctions and extracellular matrix (ECM) intact, cell sheet technology has emerged as a ground-breaking approach. Investigating the possibility of using sodium selenite (as medium supplementation) and PCL-PEG-PCL (as vessel coating substrate) in the formation of the sheets from rat bone marrow-derived mesenchymal stem cells (rBMSCs) was the main goal of the present study. To this end, first, Polycaprolactone-co-Poly (ethylene glycol)-co-Polycaprolactone triblock copolymer (PCEC) was prepared by ring-opening copolymerization method and characterized by FTIR, 1  H NMR, and GPC...
April 6, 2023: Journal of Biological Engineering
https://read.qxmd.com/read/36753038/mpv17-mutation-related-mitochondrial-dna-depletion-syndrome-a-case-series-in-infants
#18
JOURNAL ARTICLE
Arghya Samanta, Anshu Srivastava, Kausik Mandal, Moinak Sen Sarma, Ujjal Poddar
MPV17 is a mitochondrial inner membrane protein, involved in transporting deoxynucleotides into the mitochondria. Pathogenic MPV17 mutations can cause mitochondrial deoxyribonucleic acid (DNA) depletion syndrome, which has a varied presentation with neurological, muscular and hepatic involvement. Presentation as liver failure is relatively uncommon. Here, we report four infants from four separate families with pathogenic, homozygous MPV17 mutations. All had predominant hepatic involvement with cholestasis, lactic acidosis and hypoketotic hypoglycemia...
February 8, 2023: Indian Journal of Gastroenterology: Official Journal of the Indian Society of Gastroenterology
https://read.qxmd.com/read/36711823/oligonucleotide-directed-proximity-interactome-mapping-o-map-a-unified-method-for-discovering-rna-interacting-proteins-transcripts-and-genomic-loci-in-situ
#19
Ashley F Tsue, Evan E Kania, Diana Q Lei, Rose Fields, Christopher D McGann, Elliot Hershberg, Xinxian Deng, Maryanne Kihiu, Shao-En Ong, Christine M Disteche, Sita Kugel, Brian J Beliveau, Devin K Schweppe, David M Shechner
Throughout biology, RNA molecules form complex networks of molecular interactions that are central to their function, but remain challenging to investigate. Here, we introduce Oligonucleotide-mediated proximity-interactome MAPping (O-MAP), a straightforward method for elucidating the biomolecules near an RNA of interest, within its native cellular context. O-MAP uses programmable oligonucleotide probes to deliver proximity-biotinylating enzymes to a target RNA, enabling nearby molecules to be enriched by streptavidin pulldown...
January 20, 2023: bioRxiv
https://read.qxmd.com/read/36679867/effect-of-double-mutation-l452r-and-e484q-on-the-binding-affinity-of-monoclonal-antibodies-mabs-against-the-rbd-a-target-for-vaccine-development
#20
JOURNAL ARTICLE
Deepali Gupta, Mukesh Kumar, Priyanka Sharma, Trishala Mohan, Amresh Prakash, Renu Kumari, Punit Kaur
The COVID-19 pandemic, caused by SARS-CoV-2, emerges as a global health problem, as the viral genome is evolving rapidly to form several variants. Advancement and progress in the development of effective vaccines and neutralizing monoclonal antibodies are promising to combat viral infections. In the current scenario, several lineages containing "co-mutations" in the receptor-binding domain (RBD) region of the spike (S) protein are imposing new challenges. Co-occurrence of some co-mutations includes delta (L452R/T478K), kappa (L452R/E484Q), and a common mutation in both beta and gamma variants (E484K/N501Y)...
December 22, 2022: Vaccines
keyword
keyword
18761
1
2
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.