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https://www.readbyqxmd.com/read/28719949/seroprevalence-of-toxoplasmosis-with-elisa-and-rapid-diagnostic-test-among-residents-in-gyodong-do-inchon-city-korea-a-four-year-follow-up
#1
Yeong Hoon Kim, Ji Hoo Lee, Seong Kyu Ahn, Tong-Soo Kim, Sung-Jong Hong, Chom-Kyu Chong, Hye-Jin Ahn, Ho-Woo Nam
ELISA has been used for the diagnosis of toxoplasmosis, but it is being gradually replaced by a rapid diagnostic test (RDT). We compared and analyzed ELISA and RDT results using the sera collected during 4 consecutive years from residents of Gyodong-do (Island), Incheon-city, Korea. Sera from 921, 993, 940, and 838 adult residents were collected on a yearly basis (2010-2013). ELISA was performed by using a crude extract of T. gondii RH strain antigen and IgG/IgM RDT mounted with recombinant fragment of major surface antigen (SAG1), GST-linker-SAG1A, were applied to the sera...
June 2017: Korean Journal of Parasitology
https://www.readbyqxmd.com/read/28719947/efficient-liquid-media-for-encystation-of-pathogenic-free-living-amoebae
#2
Hae-Jin Sohn, Heekyoung Kang, Ga-Eun Seo, Jong-Hyun Kim, Suk-Yul Jung, Ho-Joon Shin
Pathogenic Naegleria fowleri, Acanthamoeba castellanii, and Acanthamoeba polyphaga, are distributed worldwide. They are causative agents of primary amoebic meningoencephalitis or acanthamoebic keratitis in humans, respectively. Trophozoites encyst in unfavorable environments, such as exhausted food supply and desiccation. Until recently, the method of N. fowleri encystation used solid non-nutrient agar medium supplemented with heat-inactivated Escherichia coli; however, for the amoebic encystment of Acanthamoeba spp...
June 2017: Korean Journal of Parasitology
https://www.readbyqxmd.com/read/28719824/supplementation-of-l-carnitine-during-in%C3%A2-vitro-maturation-improves-embryo-development-from-less-competent-bovine-oocytes
#3
Drahomira Knitlova, Pavlina Hulinska, Michal Jeseta, Katerina Hanzalova, Bartosz Kempisty, Marie Machatkova
The present study was designed to define the impact of l-carnitine, supplemented during maturation, on bovine oocytes with different meiotic competence in terms of their IVF outcomes. Meiotically more competent (MMC) and less competent (MLC) oocytes were obtained separately from differently sized follicles at selected phases of folliculogenesis. The oocytes were matured with or without l-carnitine, fertilized and cultured to the blastocyst stage. The oocytes were examined for nuclear maturation, mitochondrial cluster formation, lipid consumption, fertilization and embryo development...
July 5, 2017: Theriogenology
https://www.readbyqxmd.com/read/28719755/clinical-risk-factors-and-prognostic-model-for-primary-graft-dysfunction-after-lung-transplantation-in-patients-with-pulmonary-hypertension
#4
Mary K Porteous, James C Lee, David J Lederer, Scott M Palmer, Edward Cantu, Rupal J Shah, Scarlett L Bellamy, Vibha N Lama, Sangeeta M Bhorade, Maria M Crespo, John F McDyer, Keith M Wille, A Russell Localio, Jonathan B Orens, Pali D Shah, Ann B Weinacker, Selim Arcasoy, David S Wilkes, Lorraine B Ware, Jason D Christie, Steven M Kawut, Joshua M Diamond
RATIONALE: Pulmonary hypertension from pulmonary arterial hypertension or parenchymal lung disease is associated with an increased risk of primary graft dysfunction after lung transplantation. OBJECTIVE: We evaluated the clinical determinants of severe primary graft dysfunction in pulmonary hypertension and developed and validated a prognostic model. METHODS: We conducted a retrospective cohort study of patients in the multi-center Lung Transplant Outcomes Group with pulmonary hypertension at transplant listing...
July 18, 2017: Annals of the American Thoracic Society
https://www.readbyqxmd.com/read/28719753/exercise-prescriptions-for-training-and-rehabilitation-in-patients-with-heart-and-lung-disease
#5
Pietro Palermo, Ugo Corrà
Rehabilitation in patients with advanced cardiac and pulmonary disease has been shown to increase survival and improve quality of life among many other benefits. Exercise training is the fundamental ingredient in these rehabilitation programs. However, determining the amount of exercise is not straightforward or uniform. Most rehabilitation and training program fix the time of exercise and set the exercise intensity to the goals of the rehabilitation program and the exercise-related hurdles of the individual...
July 18, 2017: Annals of the American Thoracic Society
https://www.readbyqxmd.com/read/28719741/macroscopic-strain-induced-transition-from-quasi-infinite-gold-nanoparticle-chains-to-defined-plasmonic-oligomers
#6
Anja Maria Steiner, Martin Mayer, Maximilian Seuss, Svetoslav Nikolov, Kenneth D Harris, Alexander Alexeev, Christian Kuttner, Tobias A F Koenig, Andreas Fery
We investigate the formation of chains of few plasmonic nanoparticles - so called plasmonic oligomers - by strain induced fragmentation of linear particle assemblies. Detailed investigations of the fragmentation process are conducted by in-situ atomic force microscopy (AFM) and UV-vis-NIR spectroscopy. Based on these experimental results and mechanical simulations computed by the lattice spring model (LSM), we propose a formation mechanism which explains the observed decrease of chain polydispersity upon increasing strain and provides experimental guidelines for tailoring chain length distribution...
July 18, 2017: ACS Nano
https://www.readbyqxmd.com/read/28719710/conservative-management-of-postoperative-chylous-fistula-with-octreotide-and-peripheral-total-parenteral-nutrition
#7
Jason Y Chan, Eddy W Wong, S K Ng, C Andrew van Hasselt, Alexander C Vlantis
Postoperative chylous fistula after neck dissection is an uncommon complication associated with significant patient morbidity. Octreotide acetate is a somatostatin analogue established in the treatment of chylothorax; however, its utility in the management of cervical chylous fistulae has not been fully evaluated. The investigators hypothesized that chylous fistula can be managed by a combination of octreotide and peripheral total parenteral nutrition (TPN). A retrospective review of cases compiled at our institution from 2009 to 2015 was conducted...
July 2017: Ear, Nose, & Throat Journal
https://www.readbyqxmd.com/read/28719691/associations-of-weight-gain-from-early-to-middle-adulthood-with-major-health-outcomes-later-in-life
#8
Yan Zheng, JoAnn E Manson, Changzheng Yuan, Matthew H Liang, Francine Grodstein, Meir J Stampfer, Walter C Willett, Frank B Hu
Importance: Data describing the effects of weight gain across adulthood on overall health are important for weight control. Objective: To examine the association of weight gain from early to middle adulthood with health outcomes later in life. Design, Setting, and Participants: Cohort analysis of US women from the Nurses' Health Study (1976-June 30, 2012) and US men from the Health Professionals Follow-Up Study (1986-January 31, 2012) who recalled weight during early adulthood (at age of 18 years in women; 21 years in men), and reported current weight during middle adulthood (at age of 55 years)...
July 18, 2017: JAMA: the Journal of the American Medical Association
https://www.readbyqxmd.com/read/28719667/assessing-intragenomic-variation-of-the-internal-transcribed-spacer-two-adapting-the-illumina-metagenomics-protocol
#9
Lo'ai Alanagreh, Caitlin Pegg, Amritha Harikumar, Mark Buchheim
Primary and secondary structural data from the internal transcribed spacer two (ITS2) have been used extensively for diversity studies of many different eukaryotic organisms, including the green algae. Ease of amplification is due, at least in part, to the fact that ITS2 is part of the tandemly-repeated rRNA array. The potential confounding influence of intragenomic variability has yet to be addressed except in a few organisms. Moreover, few of the assessments of intragenomic variation have taken advantage of the deep sequencing capacity of sequence-by-synthesis protocols...
2017: PloS One
https://www.readbyqxmd.com/read/28719625/differences-of-immune-disorders-between-alzheimer-s-disease-and-breast-cancer-based-on-transcriptional-regulation
#10
Wei Kong, Xiaoyang Mou, Jin Deng, Benteng Di, Ruxing Zhong, Shuaiqun Wang, Yang Yang, Weiming Zeng
Although chronic inflammation and immune disorders are of great importance to the pathogenesis of both dementia and cancer, the pathophysiological mechanisms are not clearly understood. In recent years, growing epidemiological evidence and meta-analysis data suggest an inverse association between Alzheimer's disease (AD), which is the most common form of dementia, and cancer. It has been revealed that some common genes and biological processes play opposite roles in AD and cancer; however, the biological immune mechanism for the inverse association is not clearly defined...
2017: PloS One
https://www.readbyqxmd.com/read/28719564/the-new-apgar-score-a-checklist-to-enhance-quality-of-life-in-geriatric-patients-with-hip-fracture
#11
Joseph Bernstein, Sara Weintraub, Eric Hume, Mark D Neuman, Stephen L Kates, Jaimo Ahn
For geriatric patients with hip fractures, the broken bone is the reason for admission, but only part of the overall disease. Indeed, it may be more helpful to consider the patient having geriatric hip fracture syndrome or sustaining a hip attack, as there are many associated medical, social, psychological, and other problems to which attention must be paid. To that end, we have identified a series of 10 steps, collected into a checklist, that can be undertaken for all patients with geriatric hip fracture. In homage to the maxim "we come into the world under the brim of the pelvis and go out through the neck of the femur," we defined our checklist by the acronym APGAR SCORE, named after the classic checklist of the same name used to assess a newborn child...
July 19, 2017: Journal of Bone and Joint Surgery. American Volume
https://www.readbyqxmd.com/read/28719561/arterial-topographic-anatomy-near-the-femoral-head-neck-perforation-with-surgical-relevance
#12
Paulo Rego, Vasco Mascarenhas, Diego Collado, Ana Coelho, Luis Barbosa, Reinhold Ganz
BACKGROUND: Knowledge of the vascular supply of the femoral head is crucial for hip-preserving surgical procedures. The critical area for reshaping cam deformity is at the retinacular vessel penetration, an area with ill-defined topographic anatomy. We performed a cadaver study of the extension of the lateral retinaculum near the head-neck junction, distribution of the arterial vascular foramina, and initial intracapital course of these vessels. METHODS: In 16 fresh proximal parts of the femur without head-neck deformities, the deep branch of the medial femoral circumflex artery was injected with gadolinium for magnetic resonance imaging (MRI) sequences to identify arterial structures...
July 19, 2017: Journal of Bone and Joint Surgery. American Volume
https://www.readbyqxmd.com/read/28719560/perioperative-and-delayed-major-complications-following-surgical-treatment-of-adolescent-idiopathic-scoliosis
#13
Carrie E Bartley, Burt Yaszay, Tracey P Bastrom, Suken A Shah, Baron S Lonner, Jahangir Asghar, Firoz Miyanji, Amer Samdani, Peter O Newton
BACKGROUND: Reporting accurate surgical complication rates to patients and their families is important in the management of adolescent idiopathic scoliosis (AIS). In this study, we report the rate of major complications following the surgical treatment of AIS both in the perioperative period and among patients with a minimum of 2 years of follow-up. METHODS: We reviewed the prospectively collected data of a multicenter registry of patients who underwent surgical treatment of AIS during the period of 1995 to 2014 in order to identify all complications...
July 19, 2017: Journal of Bone and Joint Surgery. American Volume
https://www.readbyqxmd.com/read/28719557/functional-gain-and-pain-relief-after-total-joint-replacement-according-to-obesity-status
#14
Wenjun Li, David C Ayers, Courtland G Lewis, Thomas R Bowen, Jeroan J Allison, Patricia D Franklin
BACKGROUND: Obesity has been associated with lower function and more pain before and after total hip or knee replacement (THR or TKR). We examined the changes between preoperative and postoperative function and pain in a large representative U.S. cohort to determine if there was a relationship to obesity status. METHODS: Preoperative and 6-month postoperative data on function (Short Form-36 Physical Component Summary [PCS] score), joint pain (Hip disability and Osteoarthritis Outcome Score and Knee injury and Osteoarthritis Outcome Score), and body mass index (BMI) were collected from a national sample of 2,040 patients who had undergone THR and 2,964 who had undergone TKR from May 2011 to March 2013...
July 19, 2017: Journal of Bone and Joint Surgery. American Volume
https://www.readbyqxmd.com/read/28719555/the-influence-of-shortening-on-clinical-outcome-in-healed-displaced-midshaft-clavicular-fractures-after-nonoperative-treatment
#15
E B Goudie, N D Clement, I R Murray, C R Lawrence, M Wilson, A J Brooksbank, C M Robinson
BACKGROUND: We aimed to evaluate the effect of clavicular shortening, measured with 3-dimensional computed tomography (3DCT), on functional outcomes and satisfaction in patients with healed displaced midshaft clavicular fractures up to 1 year following injury. METHODS: The data used in this study were collected as part of a multicenter, prospective randomized controlled trial of open reduction and plate fixation compared with nonoperative treatment for acute, displaced midshaft clavicular factures...
July 19, 2017: Journal of Bone and Joint Surgery. American Volume
https://www.readbyqxmd.com/read/28719548/forearm-pronation-osteotomy-for-supination-contracture-secondary-to-obstetrical-brachial-plexus-palsy-a-retrospective-cohort-study
#16
Aharon Z Gladstein, Brant Sachleben, Emily S Ho, Alison Anthony, Howard M Clarke, Sevan Hopyan
BACKGROUND: Obstetrical brachial plexus palsy can lead to fixed forearm supination contracture. Fixed supination may lead to functional deficits as the affected hand cannot be positioned optimally for activities on a desk such as writing and typing, or for using tools including utensils, which require a neutral or pronated forearm. Forearm pronation osteotomy has been used to address this problem, although the functional benefit over nonoperative management has not been clearly defined...
July 17, 2017: Journal of Pediatric Orthopedics
https://www.readbyqxmd.com/read/28719542/cideb-deficiency-aggravates-dextran-sulfate-sodium-induced-ulcerative-colitis-in-mice-by-exacerbating-the-oxidative-burden-in-colonic-mucosa
#17
Chao Sun, Yuanlin Zhao, Xing Gao, Yuan Yuan, Chao Wang, Yingmei Wang, Lijun Zhang, Yu Gu, Feng Zhang, Peizhen Hu, Zhe Wang, Zhuyi Li, Jiankuan Shi, Sheng Li, Kaiyu Cao, Bingcheng Ren, Ming Yu, Weihua Liang, Zengshan Li, Jing Ye
BACKGROUND: Abnormal lipid metabolism is one of many factors that contribute to the development of ulcerative colitis (UC). As a lipid droplet-associated protein, Cideb facilitated the export of lipids from enterocytes and promoted intestinal lipid absorption. We found that Cideb was upregulated in the colonic mucosa of both UC patients and dextran sodium sulfate (DSS)-induced mouse colitis, but its roles in the pathogenesis of UC are still ill-defined. METHODS: Acute colitis was induced with DSS in Cideb-null and wild-type mice, and the inflammation and oxidative stress were evaluated in the colonic mucosa...
August 2017: Inflammatory Bowel Diseases
https://www.readbyqxmd.com/read/28719539/inflammatory-bowel-disease-pharmacist-adherence-counseling-improves-medication-adherence-in-crohn-s-disease-and-ulcerative-colitis
#18
Darren K Tiao, Webber Chan, Jayson Jeganathan, Jenny T Chan, Jonathan Perry, Christian P Selinger, Rupert W Leong
BACKGROUND: While inflammatory bowel diseases (IBD) require long-term medication usage to maintain remission, nonadherence is common and adversely associated with poorer clinical outcomes. Personalized IBD Pharmacist Adherence Counselling, based on the Health Beliefs Model of medication perception, may increase medication adherence. METHODS: This prospective multi-center longitudinal parallel study recruited consecutive IBD subjects that were classified as baseline medication non-adherers and adherers...
August 2017: Inflammatory Bowel Diseases
https://www.readbyqxmd.com/read/28719510/evaluation-of-the-clinical-and-laboratory-characteristics-of-previously-followed-up-thalassemia-intermedia-patients-to-provide-them-better-care-in-the-future
#19
Özlem Tüfekçi, Berna Atabay, Meral Türker, Şebnem Yilmaz Bengoa, Salih Gözmen, Tuba Karapinar, Hale Ören, Gülersu İrken
The increased awareness about the severity of complications in thalassemia intermedia patients led authorities to develop strategies for better management and follow-up of these patients. In this study, we aimed to define the clinical and laboratory characteristics in previously followed-up β-thalassemia intermedia patients and wanted to gain an insight about the follow-up of this patient population in a developing country to provide them better care in the future. The mean age at diagnosis was 4 years, and the mean hemoglobin was 7...
July 17, 2017: Journal of Pediatric Hematology/oncology
https://www.readbyqxmd.com/read/28719505/prevalence-of-persistent-renal-dysfunction-in-perinatally-hiv-infected-thai-adolescents
#20
Torsak Bunupuradah, Tanit Phupitakphol, Jiratchaya Sophonphan, Wasana Prasitsuebsai, Suvaporn Anugulruengkitt, Watsamon Jantarabenjakul, Bunruan Sopa, Kiat Ruxrungtham, Ankanee Chanakul, Thanyawee Puthanakit
BACKGROUND: Persistent renal dysfunction (PRD) has been reported in up to 22% of perinatally HIV-infected adolescents (PHA) in the US and Europe. There is limited data available on PRD among PHAs in resource-limited settings regarding access to antiretroviral therapy (ART) at more advanced HIV stages. METHODS: We retrospectively described the prevalence of PRD and associated factors in a Thai PHA cohort. Inclusion criteria were current age >10 years and at least 2 serum creatinine (Cr) measurements after ART initiation...
July 13, 2017: Pediatric Infectious Disease Journal
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