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https://www.readbyqxmd.com/read/28439618/-radiofrequency-current-or-cryoballoon-for-ablation-of-atrial-fibrillation-hot-or-cold
#1
B Reissmann, K-H Kuck, A Metzner
Atrial fibrillation (AF) is the most common form of cardiac arrhythmia. The aim of therapy in symptomatic patients is the establishment of a stable sinus rhythm (SR). Catheter ablation with isolation of the pulmonary veins is the essential component of all forms of ablation therapy and provides the most effective treatment option. The most frequently used technologies for pulmonary vein isolation (PVI) are radiofrequency current (RFC)-based and cryoballoon (CB)-guided ablation. Irrespective of the simplification of PVI, CB ablation is characterized by a short learning curve and short procedural times and demonstrated non-inferiority with respect to safety and efficacy when directly compared to RFC ablation for the treatment of patients with paroxysmal AF; however, the clinical outcome in patients with persistent AF is often insufficient when performing pulmonary vein isolation (PVI) alone for stabilization of SR...
April 24, 2017: Herz
https://www.readbyqxmd.com/read/28439458/the-genomic-sequence-of-exiguobacterium-chiriqhucha-str-n139-reveals-a-species-that-thrives-in-cold-waters-and-extreme-environmental-conditions
#2
Ana Gutiérrez-Preciado, Carlos Vargas-Chávez, Mariana Reyes-Prieto, Omar F Ordoñez, Diego Santos-García, Tania Rosas-Pérez, Jorge Valdivia-Anistro, Eria A Rebollar, Andrés Saralegui, Andrés Moya, Enrique Merino, María Eugenia Farías, Amparo Latorre, Valeria Souza
We report the genome sequence of Exiguobacterium chiriqhucha str. N139, isolated from a high-altitude Andean lake. Comparative genomic analyses of the Exiguobacterium genomes available suggest that our strain belongs to the same species as the previously reported E. pavilionensis str. RW-2 and Exiguobacterium str. GIC 31. We describe this species and propose the chiriqhucha name to group them. 'Chiri qhucha' in Quechua means 'cold lake', which is a common origin of these three cosmopolitan Exiguobacteria. The 2,952,588-bp E...
2017: PeerJ
https://www.readbyqxmd.com/read/28437432/genome-wide-identification-and-expression-analysis-of-calcium%C3%A2-dependent-protein-kinase-and-its-related-kinase-gene-families-in-melon-cucumis-melo-l
#3
Haifei Zhang, Chunhua Wei, Xiaozhen Yang, Hejie Chen, Yongchao Yang, Yanling Mo, Hao Li, Yong Zhang, Jianxiang Ma, Jianqiang Yang, Xian Zhang
The calcium-dependent protein kinase (CDPK) is a ser/thr protein kinase that plays vital roles in plant growth, development, and responses to multiple stresses. Despite an important member of the stress responsive gene family, little is known about the evolutionary history and expression patterns of CDPK genes in melon. Herein, a total of 18 CDPK genes and 7 CDPK-related protein kinases (CRK) genes were identified in the melon genome via bioinformatic analysis, which were unevenly distributed across eleven chromosomes with an apparent exception for chromosome 3...
2017: PloS One
https://www.readbyqxmd.com/read/28435574/subungual-glomus-tumours-is-magnetic-resonance-imaging-or-ultrasound-necessary-for-diagnosis
#4
C R Pandey, N Singh, B Tamang
INTRODUCTION: Diagnosis of subungual glomus tumour is mostly based on detailed history and clinical examination. Recently, Magnetic Resonance Imaging (MRI) and Ultrasound have been proposed as the imaging modality to confirm the clinical diagnosis and in planning the surgical management of these tumours. However, these imaging modalities are not routinely available in rural setting and also are expensive. Due to these limitations, we set out to establish that diagnosis and management of these rare tumours can be based solely on a battery of clinical tests and history taking...
March 2017: Malaysian Orthopaedic Journal
https://www.readbyqxmd.com/read/28432838/are-poikilothermic-animals-real-hosts-for-toxoplasma-gondii
#5
Henryka Długońska
The protozoan Toxoplasma gondii is believed to be a common parasite of almost all endothermic animals and humans. However, recent reports of toxoplasmosis in marine mammals raise concern that cold-blooded animals may also be a potential source of T. gondii infection. The article discusses the presence of T. gondii in aquatic and terrestrial poikilothermic animals, which may be important elements in the transmission of the parasite.
2017: Annals of Parasitology
https://www.readbyqxmd.com/read/28431814/evaluation-of-storing-hepatitis-b-vaccine-outside-the-cold-chain-in-the-solomon-islands-identifying-opportunities-and-barriers-to-implementation
#6
Lucy Breakwell, Jenniffer Anga, Ibrahim Dadari, Nahad Sadr-Azodi, Divinal Ogaoga, Minal Patel
Monovalent Hepatitis B vaccine (HepB) is heat stable, making it suitable for storage outside cold chain (OCC) at 37°C for 1month. We conducted an OCC project in the Solomon Islands to determine the feasibility of and barriers to national implementation and to evaluate impact on coverage. Healthcare workers at 13 facilities maintained monovalent HepB birth dose (HepB-BD) OCC for up to 28days over 7months. Vaccination data were recorded for children born during the project and those born during 7months before the project...
April 18, 2017: Vaccine
https://www.readbyqxmd.com/read/28431099/effect-of-cold-water-induced-stress-on-immune-response-pathology-and-fertility-in-mice-during-chlamydia-muridarum-genital-infection
#7
Tesfaye Belay, Anthony Woart, Vincent Graffeo
Genital infection by Chlamydia trachomatis (CT) is the most common bacterial sexually-transmitted disease worldwide. It causes serious reproductive health complications, including pelvic inflammatory disease and infertility. Stress is implicated as a risk factor for various infections; however, its effect on chlamydia genital infection is unknown. We previously showed that repeated exposure of mice to cold water results in increased severity of chlamydia genital infection. In the present study, cold water-induced stress resulted in: i) elevated levels of NE and EP in the spleen and genital tract of stressed mice; ii) elevated IL-1β, TNF-α, IL-6 and NO production in macrophage-rich peritoneal cells of mice; iii) supplement of NE in vitro exerts an immunosuppressive effect on splenic T cell production of cytokines; iv) decreased C...
April 18, 2017: Pathogens and Disease
https://www.readbyqxmd.com/read/28429269/alternative-carbon-dioxide-modelling-approaches-accounting-for-high-residual-gases-in-landgem
#8
Nathan Bruce, Kelvin Tsun Wai Ng, Amy Richter
High Canadian waste disposal rates necessitate landfill gas monitoring and accurate forecasting. CO2 estimates in LandGEM version 3.02 currently rest on the assumptions that CO2 is a function of CH4, where the two gases make up nearly 100% of landfill gas content, leading to overestimated CO2 collection estimates. A total of 25 cases (five formulas, five approaches) compared annual CO2 collection at four western Canadian landfills. Despite common use in literature, the 1:1 ratio of CH4 to CO2 was not recommended to forecast landfill gas collection in cold climates...
April 20, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28426293/identifying-thermal-breakdown-products-of-thermoplastics
#9
Marianne Guillemot, Benoît Oury, Sandrine Melin
Polymers processed to produce plastic articles are subjected to temperatures between 150°C and 450°C or more during overheated processing and breakdowns. Heat-based processing of this nature can lead to emission of volatile organic compounds (VOCs) into the thermoplastic processing shop. In this study, laboratory experiments, qualitative and quantitative emissions measurement in thermoplastic factories were carried out. The first step was to identify the compounds released depending on the thermoplastic nature, the temperature and the type of process...
April 20, 2017: Journal of Occupational and Environmental Hygiene
https://www.readbyqxmd.com/read/28425458/anatomy-of-terminal-moraine-segments-and-implied-lake-stability-on-ngozumpa-glacier-nepal-from-electrical-resistivity-tomography-ert
#10
Sarah S Thompson, Bernd Kulessa, Douglas I Benn, Jordan R Mertes
Moraine-dammed lakes at debris-covered glaciers are becoming increasingly common and pose significant outburst flood hazards if the dam is breached. While moraine subsurface structure and internal processes are likely to influence dam stability, only few sites have so far been investigated. We conducted electrical resistivity tomography (ERT) surveys at two sites on the terminal moraine complex of the Ngozumpa Glacier, Nepal, to aid assessment of future terminus stability. The resistivity signature of glacier ice at the site (100-15 kΩ m) is more consistent with values measured from cold glacier ice and while this may be feasible, uncertainties in the data inversion introduce ambiguity to this thermal interpretation...
April 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28425266/nitroglycerin-challenge-induces-lateralized-headache-in-nasociliarynerve-ligated-rats-implications-for-chronic-migraine
#11
Didem Akçali, Ahmet Deniz Belen, Avni Babacan, Hayrunnisa Bolay
BACKGROUND/AIM: Chronic migraine is a common debilitating disease with limited treatment options. We aimed to develop a novel model for chronic migraine by ligating the nasociliary nerve (NCL) and administering nitroglycerin (NTG) to exacerbate acute headache attacks. MATERIALS AND METHODS: Exacerbation of the headache was induced by NTG (10 mg/kg, subcutaneously) administered to male Wistar rats (n = 36) 14 days following unilateral NCL. Cutaneous and cold allodynia was tested using Von Frey (VF) filaments and acetone, respectively...
April 18, 2017: Turkish Journal of Medical Sciences
https://www.readbyqxmd.com/read/28424310/heart-rate-regulation-in-diving-sea-lions-the-vagus-nerve-rules
#12
REVIEW
Paul J Ponganis, Birgitte I McDonald, Michael S Tift, Cassondra L Williams
Recent publications have emphasized the potential generation of morbid cardiac arrhythmias secondary to autonomic conflict in diving marine mammals. Such conflict, as typified by cardiovascular responses to cold water immersion in humans, has been proposed to result from exercise-related activation of cardiac sympathetic fibers to increase heart rate, combined with depth-related changes in parasympathetic tone to decrease heart rate. After reviewing the marine mammal literature and evaluating heart rate profiles of diving California sea lions (Zalophus californianus), we present an alternative interpretation of heart rate regulation that de-emphasizes the concept of autonomic conflict and the risk of morbid arrhythmias in marine mammals...
April 15, 2017: Journal of Experimental Biology
https://www.readbyqxmd.com/read/28421275/no-association-between-hand-and-foot-temperature-responses-during-local-cold-stress-and-rewarming
#13
Lena Norrbrand, Roger Kölegård, Michail E Keramidas, Igor B Mekjavic, Ola Eiken
PURPOSE: The purpose was to examine whether associations exist between temperature responses in the fingers vs. toes and hand vs. foot during local cold-water immersion and rewarming phases. METHODS: Seventy healthy subjects (58 males, 12 females) immersed their right hand or right foot, respectively, in 8 °C water for 30 min (CWI phase), followed by a 15-min spontaneous rewarming (RW) in 25 °C air temperature. RESULTS: Temperature was lower in the toes than the fingers during the baseline phase (27...
April 18, 2017: European Journal of Applied Physiology
https://www.readbyqxmd.com/read/28421072/dysregulated-il-1%C3%AE-secretion-in-autoinflammatory-diseases-a-matter-of-stress
#14
Sonia Carta, Claudia Semino, Roberto Sitia, Anna Rubartelli
Infectious and sterile inflammation is induced by activation of innate immune cells. Triggering of toll-like receptors by pathogen-associated molecular pattern or damage-associated molecular pattern (PAMP or DAMP) molecules generates reactive oxygen species that in turn induce production and activation of pro-inflammatory cytokines such as IL-1β. Recent evidence indicates that cell stress due to common events, like starvation, enhanced metabolic demand, cold or heat, not only potentiates inflammation but may also directly trigger it in the absence of PAMPs or DAMPs...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28420706/common-structural-features-of-cholesterol-binding-sites-in-crystallized-soluble-proteins
#15
Anna N Bukiya, Alejandro M Dopico
Cholesterol-protein interactions are essential for the architectural organization of cell membranes and for lipid metabolism. While cholesterol-sensing motifs in transmembrane proteins have been identified, little is known about cholesterol recognition by soluble proteins. We reviewed the structural characteristics of binding sites for cholesterol and cholesterol sulfate from crystallographic structures available in the Protein Data Bank. This analysis unveiled key features of cholesterol-binding sites that are present in either all or the majority of sites: i) the cholesterol molecule is generally positioned between protein domains that have an organized secondary structure; ii) the cholesterol hydroxyl/sulfo group is often partnered by Asn, Gln and/or Tyr while the hydrophobic part of cholesterol interacts with Leu, Ile, Val and/or Phe; iii) cholesterol hydrogen-bonding partners are often found on alpha-helices while amino acids that interact with cholesterol hydrophobic core have a slight preference for beta-strands and secondary structure-lacking protein areas; iv) the steroid C21 and 26 constitute the ″hot spots″ most often seen for steroid-protein hydrophobic interactions; v) common ″cold spots″ are C8, 9, 10, 13 and 17, at which contacts with the proteins were not detected...
April 18, 2017: Journal of Lipid Research
https://www.readbyqxmd.com/read/28418086/general-dentists-use-of-diagnostic-equipment-and-methods
#16
Chin-Hang Kong, Michelle Davis, Kathryn Fong, Paul V Abbott
An accurate diagnosis is essential for making treatment decisions in dentistry. However, little research is available as to which tests are done routinely and how commonly they are performed. The purpose of this study was to investigate diagnostic methods and equipment used by general dentists in private practice in Perth. The study involved a retrospective audit of 30 patient records from seven individual general dentists working in private practices in the Perth Metropolitan area. De-identification of patient records was done prior to the researchers' visit...
April 18, 2017: Australian Endodontic Journal: the Journal of the Australian Society of Endodontology Inc
https://www.readbyqxmd.com/read/28411211/persistence-of-endophytic-fungi-in-cultivars-of-loliumperenne-grown-from-seeds-stored-for-22-years
#17
Gregory P Cheplick
PREMISE OF THE STUDY: Genetic resources for forage crops often consist of seeds of specific species and cultivars in cold storage for future use in breeding and selection programs. Temperate grasses such as Lolium perenne, used worldwide for forage and turf, produce seeds commonly infected by hyphae of an endophytic fungus (Epichloë festucae var. lolii). This research determined whether endophytes could persist and infect seedlings of L. perenne emerging from seeds stored for over two decades...
April 14, 2017: American Journal of Botany
https://www.readbyqxmd.com/read/28408314/targeting-endothelial-metaflammation-to-counteract-diabesity-cardiovascular-risk-current-and-perspective-therapeutic-options
#18
REVIEW
Maria Assunta Potenza, Carmela Nacci, Maria Antonietta De Salvia, Luca Sgarra, Massimo Collino, Monica Montagnani
The association of obesity and diabetes, termed "diabesity", defines a combination of primarily metabolic disorders with insulin resistance as the underlying common pathophysiology. Cardiovascular disorders associated with diabesity represent the leading cause of morbidity and mortality in the Western world. This makes diabesity, with its rising impacts on both health and economics, one of the most challenging biomedical and social threats of present century. The emerging comprehension of the genes whose alteration confers inter-individual differences on risk factors for diabetes or obesity, together with the potential role of genetically determined variants on mechanisms controlling responsiveness, effectiveness and safety of anti-diabetic therapy underlines the need of additional knowledge on molecular mechanisms involved in the pathophysiology of diabesity...
April 10, 2017: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/28404842/an-immortalized-human-dorsal-root-ganglia-cell-line-provides-a-novel-context-to-study-herpes-simplex-virus-type-1-latency-and-reactivation
#19
Nikki M Thellman, Carolyn Botting, Zachary Madaj, Steven J Triezenberg
A defining characteristic of alphaherpesviruses is the establishment of lifelong latency in host sensory ganglia with occasional reactivation causing recurrent lytic infections. As an alternative to rodent models, we explored the use of an immortalized cell line derived from human dorsal root ganglia. HD10.6 cells proliferate by virtue of a transduced tetracycline-regulated v-myc oncogene. In the presence of doxycycline, HD10.6 cells mature to exhibit neuronal morphology and express sensory neuron-associated markers such as neurotrophin receptors TrkA, TrkB, TrkC, and RET and the sensory neurofilament peripherin...
April 12, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28401298/neurovascular-hand-symptoms-in-relation-to-cold-exposure-in-northern-sweden-a-population-based-study
#20
Albin Stjernbrandt, Bodil Björ, Martin Andersson, Lage Burström, Ingrid Liljelind, Tohr Nilsson, Ronnie Lundström, Jens Wahlström
PURPOSE: To describe the self-reported ambient cold exposure in northern Sweden and to relate the level of cumulative cold exposure to the occurrence of sensory and vascular hand symptoms. We hypothesize that cold exposure is positively related to reporting such symptoms. METHODS: A questionnaire about cold exposure and related symptoms was sent out to 35,144 subjects aged 18-70 years and living in northern Sweden. RESULTS: A total of 12,627 out of 35,144 subjects returned the questionnaire (response rate 35...
April 11, 2017: International Archives of Occupational and Environmental Health
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