keyword
https://read.qxmd.com/read/33416495/methotrexate-attenuates-vascular-inflammation-through-an-adenosine-microrna-dependent-pathway
#21
JOURNAL ARTICLE
Dafeng Yang, Stefan Haemmig, Haoyang Zhou, Daniel Pérez-Cremades, Xinghui Sun, Lei Chen, Jie Li, Jorge Haneo-Mejia, Tianlun Yang, Ivana Hollan, Mark W Feinberg
Endothelial cell (EC) activation is an early hallmark in the pathogenesis of chronic vascular diseases. MicroRNA-181b ( Mir181b ) is an important anti-inflammatory mediator in the vascular endothelium affecting endotoxemia, atherosclerosis, and insulin resistance. Herein, we identify that the drug methotrexate (MTX) and its downstream metabolite adenosine exert anti-inflammatory effects in the vascular endothelium by targeting and activating Mir181b expression. Both systemic and endothelial-specific Mir181a2b2 -deficient mice develop vascular inflammation, white adipose tissue (WAT) inflammation, and insulin resistance in a diet-induced obesity model...
January 8, 2021: ELife
https://read.qxmd.com/read/33313203/jak-stat-signaling-pathway-mediated-microrna-181b-promoted-blood-brain-barrier-impairment-by-targeting-sphingosine-1-phosphate-receptor-1-in-septic-rats
#22
JOURNAL ARTICLE
Sheng-Long Chen, Geng-Xin Cai, Hong-Guang Ding, Xin-Qiang Liu, Zhong-Hua Wang, Yuan-Wen Jing, Yong-Li Han, Wen-Qiang Jiang, Miao-Yun Wen
Background: Blood-brain barrier (BBB) impairment plays a significant role in the pathogenesis of sepsis-associated encephalopathy (SAE). However, the molecular mechanisms are poorly understood. In the present study, we aimed to investigate the regulatory relationship between the Janus kinase/signal transducers and activators of transcription (JAK/STAT) signaling pathway, microRNA (miR)-181b and its target genes in sepsis in vivo and in vitro . Methods: Four rat models (sham, sepsis, sepsis plus STAT3 inhibitor (Stattic), and sepsis plus miR-181b inhibitor [sepsis + anta-miR-181b]) were established...
November 2020: Annals of Translational Medicine
https://read.qxmd.com/read/32850833/circular-rna-cmto1-promotes-pten-expression-through-sponging-mir-181b-5p-in-liver-fibrosis
#23
JOURNAL ARTICLE
Hui Jin, Chunxue Li, Peihong Dong, Junting Huang, Jinglu Yu, Jianjian Zheng
Background: Circular RNAs (circRNAs) are considered as key regulators of cancer biology. Recently, cMTO1 (a circRNA derived from MTO1 gene, hsa_circ_0007874) has been demonstrated to act as a tumor suppressor in hepatocellular carcinoma (HCC). However, the roles of cMTO1 in liver fibrosis are largely unknown. Methods: Expressions and roles of cMTO1 were examined in vivo and in vitro during liver fibrosis. The interaction between microRNA-181b-5p (miR-181b-5p) and cMTO1 was analyzed by luciferase activity assays and pull down assays...
2020: Frontiers in Cell and Developmental Biology
https://read.qxmd.com/read/31853799/lncrna-nuclear-enriched-abundant-transcript-1-regulates-hypoxia-evoked-apoptosis-and-autophagy-via-mediation-of-microrna-181b
#24
JOURNAL ARTICLE
Ying Lv, Zhaoming Liu, Jiancheng Huang, Jie Yu, Yanbo Dong, Jun Wang
Nuclear-enriched abundant transcript 1 (NEAT1), a vital long noncoding RNA (lncRNA), exhibits the functions in disparate cancers. Nevertheless, the influences of NEAT1 in congenital heart disease (CHD) remain unreported. The research delves into whether NEAT1 affects H9c2 cells apoptosis and autophagy under the hypoxia condition. Overexpressed NEAT1 vector was transfected into H9c2 cells; then, functions of NEAT1 in cell viability, apoptosis, autophagy, PI3K/AKT/mTOR and JAK1/STAT3 pathways were detected in H9c2 cells under hypoxia condition...
December 18, 2019: Molecular and Cellular Biochemistry
https://read.qxmd.com/read/31807005/lncrna-ccat1-promotes-colorectal-cancer-tumorigenesis-via-a-mir-181b-5p-tusc3-axis
#25
JOURNAL ARTICLE
Si Chen, Yan Liu, Yuanyuan Wang, Zhaoping Xue
Aim: The aim was to determine the function and molecular mechanism of long non-coding RNA colon cancer associated transcript-1(lncRNA CCAT1) in the development of colorectal cancer (CRC). Methods: CCAT1 mRNA expression levels were determined in CRC tissues and cells using reverse transcription-quantitative polymerase chain reaction. Cell Counting Kit-8 and colony formation assays were used to examine the effects of CCAT1 on the proliferation of CRC cells. Luciferase reporter gene analysis was used to confirm the target gene of microRNA-181b-5p (miR-181b-5p) in CRC cells...
2019: OncoTargets and Therapy
https://read.qxmd.com/read/31790896/liquiritigenin-suppresses-the-activation-of-hepatic-stellate-cells-via-targeting-mir-181b-pten-axis
#26
JOURNAL ARTICLE
Wujun Geng, Guangyao Zhou, Binyu Zhao, Qingqing Xiao, Chunxue Li, Sinuo Fan, Peihong Dong, Jianjian Zheng
BACKGROUND: Liquiritigenin (LQ), an aglycone of liquiritin in licorice, has demonstrated antioxidant, anti-inflammatory and anti-tumor activities. Previously, LQ was found to inhibit liver fibrosis progression. PURPOSE: Phosphatase and tensin homolog (PTEN) has been reported to act as a negative regulator of hepatic stellate cell (HSC) activation. However, the roles of PTEN in the effects of LQ on liver fibrosis have not been identified to date. METHODS: The effects of LQ on liver fibrosis in carbon tetrachloride (CCl4 ) mice as well as primary HSCs were examined...
October 4, 2019: Phytomedicine
https://read.qxmd.com/read/31632547/microrna-181b-blocks-gensenoside-rg3-mediated-tumor-suppression-of-gallbladder-carcinoma-by-promoting-autophagy-flux-via-crebrf-creb3-pathway
#27
JOURNAL ARTICLE
Keren Wu, Jie Huang, Tao Xu, Zhipeng Ye, Fa Jin, Ning Li, Bin Lv
BACKGROUND: Gallbladder cancer (GBC) is the seventh most common gastrointestinal cancer. Suppression of autophagy contributes to cell death of gallbladder cancer. Gensenoside Rg3 sensitizes tumor cells to chemotherapeutic agents through autophagy inhibition. However, its role mechanism on the progression of GBC remains vague. The present study is aimed to explore the functional action of Rg3 on GBC progression. METHODS: Expression of miR-181b and CREBRF in human gallbladder carcinoma specimen were determined by western blotting and qRT-PCR...
2019: American Journal of Translational Research
https://read.qxmd.com/read/31441410/-protective-effects-of-microrna-181b-on-aged-rats-with-sepsis-induced-hippocampus-injury-in-vivo
#28
JOURNAL ARTICLE
Rui Dong, Dan Hu, Qingshu Li, Tangwen Xu, Yanyan Sui, Yan Qu
OBJECTIVE: To investigate the protective effect of microRNA-181b (miR-181b) on aged rats with sepsis-induced hippocampus injury in vivo. METHODS: Seventy-five male healthy old Sprague-Dawley (SD) rats were randomly divided into five groups (n = 15) using a random number table: sham operation group (Sham group), sepsis group [cecal ligation and puncture (CLP) group], miR-181b Agomir+CLP group (Ag+CLP group), miR-181b Antagomir+CLP group (An+CLP group) and normal saline (NS) control group (NS+CLP group)...
July 2019: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://read.qxmd.com/read/31131439/microrna-181b-5p-modulates-tumor-necrosis-factor-%C3%AE-induced-inflammatory-responses-by-targeting-interleukin-6-in-cementoblasts
#29
JOURNAL ARTICLE
Xiaoxuan Wang, Hualing Sun, Huan Liu, Li Ma, Chenxi Jiang, Haiqing Liao, Shihan Xu, Junbo Xiang, Zhengguo Cao
Tooth cementum is a bone-like mineralized tissue and serves as a microbial barrier against invasion and destruction. Cementum is also responsible for tooth stability and defending pulp from outside stimuli, which is formed by cementoblasts. Although it is crucial for periodontal and periapical diseases, the mechanisms underlying the pathophysiological changes of cementoblasts and their inflammatory responses remain unclear. MiR-181b is found to modulate vascular inflammation and endotoxin tolerance. In this study, miR-181b-5p was downregulated in tumor necrosis factor-α (TNF-α)-stimulated cementoblasts, whereas proinflammatory molecules increased...
May 26, 2019: Journal of Cellular Physiology
https://read.qxmd.com/read/31003188/microrna-181b-5p-attenuates-early-postoperative-cognitive-dysfunction-by-suppressing-hippocampal-neuroinflammation-in-mice
#30
JOURNAL ARTICLE
Yayuan Lu, Xin Xu, Rui Dong, Lingling Sun, Lei Chen, Zongze Zhang, Mian Peng
BACKGROUND: Postoperative cognitive dysfunction (POCD) is a common complication after surgery and its occurrence is associated with increased morbidity and mortality. However, the pathophysiology of this complication remains largely unknown. Efforts to identify causes of POCD have focused on the hippocampal neuroinflammation. Recently, accumulated evidence indicates that NeurimmiRs, a subset of microRNAs (miRNAs), which modulate both neuronal and immune processes, play an important role in neuroinflammation...
April 16, 2019: Cytokine
https://read.qxmd.com/read/30977354/interleukin-1%C3%AE-nuclear-factor-%C3%AE%C2%BAb-signaling-promotes-osteosarcoma-cell-growth-through-the-microrna-181b-phosphatase-and-tensin-homolog-axis
#31
JOURNAL ARTICLE
Weiguo Wang, Zhengguang Wang, Shijie Chen, Xiaofang Zang, Jinglei Miao
So far, microRNA has attracted plenty of interest due to its role in tumorigenesis. Reportedly, miR-181b may be involved in the tumorigenesis of osteosarcoma (OS). In the current study, we attempted to investigate the detailed function and mechanism of miR-181b in OS carcinogenesis. Herein, miR-181a, miR-181b, miR-181c, and miR-181d expressions in OS tissues were higher than that in nontumor tissue samples as examined real-time polymerase chain reaction. Via direct targeting, miR-181b negatively regulated the expression of phosphatase and tensin homolog (PTEN), a well-known tumor suppressor...
February 2019: Journal of Cellular Biochemistry
https://read.qxmd.com/read/30937907/dysregulation-of-microrna-181b-and-timp3-is-functionally-involved-in-the-pathogenesis-of-diabetic-nephropathy
#32
JOURNAL ARTICLE
Fu-Xiang Zhu, Heng-Lan Wu, Jian-Xiang Chen, Bin Han, Yin-Feng Guo
This study aimed to study the roleof microRNA (miR)-181b and its target TIMP3 in the development of diabetic nephropathy (DMN) via inhibiting the apoptosis of mesangial cells. Real-time polymerase chain reaction (RT-PCR) was adopted to compare the miR-181b expression between subjects with diabetic nephropathy (DN) and normal control. In addition, luciferase assays were utilized to explore the regulatory relationship between TIMP3 and miR-181b. Real-time PCR and densitometry analysis were conducted to measure the levels of TIMP3 mRNA/protein in DMN or in cells treated by miR-181b inhibitors, miR-181b mimics, and TIMP3 siRNA...
April 1, 2019: Journal of Cellular Physiology
https://read.qxmd.com/read/30580904/microrna-181b-suppresses-the-metastasis-of-lung-cancer-cells-by-targeting-sex-determining-region-y-related-high-mobility-group-box-6-sox6
#33
JOURNAL ARTICLE
You Zhou, Xiao Zheng, Lu-Jun Chen, Bin Xu, Jing-Ting Jiang
BACKGROUND: The aim of the study was to measure the expression of microRNA (miR)-181b in patients with lung cancer, investigate its biological function and elucidate the underlying mechanisms associated with the development of lung cancer. METHODS: miR-181b expression in tissues was measured via RT-qPCR. After A549 cells were transfected with miR-181b mimic or si-Sox6, the proliferation, migration and cell cycle distribution of A549 were evaluated using cell counting kit-8 assay, transwell assay and flow cytometry...
December 11, 2018: Pathology, Research and Practice
https://read.qxmd.com/read/30431062/mir-181b-5p-suppresses-starvation-induced-cardiomyocyte-autophagy-by-targeting-hspa5
#34
JOURNAL ARTICLE
Liuhui Chang, Xiaoming Chai, Peiming Chen, Jianfang Cao, Hong Xie, Jiang Zhu
This study aimed to investigate the role of microRNA‑181b‑5p (miR‑181b‑5p) in starvation‑induced cardiomyocyte autophagy by targeting heat shock protein family A member 5 (Hspa5). For this purpose, H9c2 cardiomyocytes and neonatal rat ventricular myocytes (NRVMs) were glucose‑starved in Earle's Balanced Salt Solution (EBSS) for different periods of time (0, 2, 4, 6 and 8 h). RT‑qPCR analysis was performed to examine the expression of miR‑181b‑5p in the different groups. Immunofluorescence was performed to detect the expression of LC3...
January 2019: International Journal of Molecular Medicine
https://read.qxmd.com/read/29807144/neuroprotective-effects-of-isosteviol-sodium-through-increasing-cyld-by-the-downregulation-of-mirna-181b
#35
JOURNAL ARTICLE
Hao Zhang, Kailun Zhong, Minyi Lu, Ying Mei, Evina Tan, Xiaoou Sun, Wen Tan
NF-κB signaling pathway plays a critical role in cerebral ischemic stroke. MicroRNA-181b (miR-181b) induces the expression of NF-κB signaling pathways indirectly, and isosteviol sodium (STVNa) protects against ischemic injury via the inhibition of NF-κB-mediated inflammatory and apoptotic responses. However, the function of miR-181b and the actual relationship between STVNa and miR-181b in the ischemia-induced activation of NF-κB signaling pathways remains unclear. In this study, we found that miR-181b expression was significantly decreased in N2A neuroblastoma cells after CoCl2 -induced hypoxic injury in vitro...
June 2018: Brain Research Bulletin
https://read.qxmd.com/read/29705934/exosomes-secreted-by-adipose-derived-stem-cells-contribute-to-angiogenesis-of-brain-microvascular-endothelial-cells-following-oxygen-glucose-deprivation-in-vitro-through-microrna-181b-trpm7-axis
#36
JOURNAL ARTICLE
Yujia Yang, Yue Cai, Yuan Zhang, Juan Liu, Zhiqiang Xu
Adipose-derived stem cells (ADSCs) have been demonstrated to promote cerebral vascular remodeling processes after stroke. However, the exact molecular mechanism by which ADSCs exert protective roles in ischemic stroke is still poorly understood. In this study, we identified the role of exosomal microRNA-181b-5p (181b-Exos) in regulating post-stroke angiogenesis. The results of migration assay and capillary network formation assay showed that exosomes secreted by ADSCs (ADSCs-Exos) promoted the mobility and angiogenesis of brain microvascular endothelial cells (BMECs) after oxygen-glucose deprivation (OGD)...
May 2018: Journal of Molecular Neuroscience: MN
https://read.qxmd.com/read/29434967/celastrol-suppresses-the-proliferation-of-lung-adenocarcinoma-cells-by-regulating-microrna-24-and-microrna-181b
#37
JOURNAL ARTICLE
Yun-Fei Yan, Han-Han Zhang, Qing Lv, Yue-Mei Liu, You-Jie Li, Bao-Sheng Li, Ping-Yu Wang, Wen-Jing Shang, Zhen Yue, Shu-Yang Xie
Cumulative evidence has indicated that celastrol may suppress cancer growth; however, the underlying mechanism requires further investigation. In the present study, A549 cells were treated with various concentrations of celastrol. Lung cancer cell proliferation was evaluated using an MTT assay and observed under a microscope. Cell apoptosis was detected by Annexin V fluorescein isothiocyanate/propidium iodide double-labeled flow cytometry. The results demonstrated that celastrol suppressed proliferation and induced apoptosis in a dose-independent manner...
February 2018: Oncology Letters
https://read.qxmd.com/read/29136944/miat-promotes-proliferation-and-hinders-apoptosis-by-modulating-mir-181b-stat3-axis-in-ox-ldl-induced-atherosclerosis-cell-models
#38
JOURNAL ARTICLE
Xiaoming Zhong, Xiang Ma, Lei Zhang, Yanming Li, Yunwei Li, Ruili He
BACKGROUND: Plenty of lncRNAs and microRNAs have been identified to be critical mediators in the progression of atherosclerosis (AS). Myocardial infarction-associated transcript (MIAT) were aberrantly high expressed and closely associated with the pathogenesis of AS. However, its molecular mechanism has not been well characterized. METHODS: The expression patterns of MIAT and microRNA-181b (miR-181b) in clinical samples and cells were measured by RT-qPCR assays...
January 2018: Biomedicine & Pharmacotherapy
https://read.qxmd.com/read/29044225/microrna-181b-is-increased-in-cystic-fibrosis-cells-and-impairs-lipoxin-a-4-receptor-dependent-mechanisms-of-inflammation-resolution-and-antimicrobial-defense
#39
JOURNAL ARTICLE
Anna Maria Pierdomenico, Sara Patruno, Marilina Codagnone, Felice Simiele, Veronica Cecilia Mari, Roberto Plebani, Antonio Recchiuti, Mario Romano
The involvement of microRNA (miR) in cystic fibrosis (CF) pathobiology is rapidly emerging. We previously documented that miR-181b controls the expression of the ALX/FPR2 receptor, which is recognized by the endogenous proresolution ligand, lipoxin (LX)A4 . Here, we examined whether the miR-181b-ALX/FPR2 circuit was altered in CF. We examined human airways epithelial cells, normal (16HBE14o-), carrying the ΔF508 mutation (CFBE41o-) or corrected for this mutation (CFBE41o-/CEP-CFTR wt 6.2 kb), as well as monocyte-derived macrophages (MΦs) from CF patients...
October 18, 2017: Scientific Reports
https://read.qxmd.com/read/28236165/mechanosensitive-microrna-181b-regulates-aortic-valve-endothelial-matrix-degradation-by-targeting-timp3
#40
JOURNAL ARTICLE
Jack M Heath, Joan Fernandez Esmerats, Lucky Khambouneheuang, Sandeep Kumar, Rachel Simmons, Hanjoong Jo
Calcific aortic valve disease (CAVD) is a major cause of morbidity in the aging population, but the underlying mechanisms of its progression remain poorly understood. Aortic valve calcification preferentially occurs on the fibrosa, which is subjected to disturbed flow. The side-specific progression of the disease is characterized by inflammation, calcific lesions, and extracellular matrix (ECM) degradation. Here, we explored the role of mechanosensitive microRNA-181b and its downstream targets in human aortic valve endothelial cells (HAVECs)...
June 2018: Cardiovascular Engineering and Technology
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