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M. Smithii

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https://www.readbyqxmd.com/read/27737924/characteristics-of-a-water-forming-nadh-oxidase-from-methanobrevibacter-smithii-an-archaeon-in-the-human-gut
#1
Mingguang Yan, Weibing Yin, Xiao Fang, Jianjun Guo, Hong Shi
NADH oxidases (NOXs) catalyzing the oxidation of NADH to yield NAD(+) and H2O, H2O2, or both play an important role in protecting organisms from oxidative stress and maintaining the balance of NAD(+)/NADH. A gene encoding NOX was identified from Methanobrevibacter smithii (NOX-ms), the predominant archaeon in the human gut ecosystem. Subsequent analyses showed that it is an FAD-containing protein with a subunit molecular mass of 48 kDa. NOX-ms was purified to homogeneity after expression in Escherichia coli NOX-ms catalyzed the oxidization of NADH and converted O2 to H2O with an optimal pH of 7...
October 13, 2016: Bioscience Reports
https://www.readbyqxmd.com/read/27697372/assessing-methanobrevibacter-smithii-and-clostridium-difficile-as-not-conventional-faecal-indicators-in-effluents-of-a-wastewater-treatment-plant-integrated-with-sludge-anaerobic-digestion
#2
Valeria Romanazzi, Silvia Bonetta, Stefania Fornasero, Margherita De Ceglia, Giorgio Gilli, Deborah Traversi
Wastewater treatment plants (WWTP) are an important source of surface water contamination by enteric pathogens, affecting the role of environmental water as a microbial reservoir. We describe the release to the environment of certain anaerobes of human and environmental concern. The work was focused on emerging microbial targets. They are tracing, by RT-qPCR, on WWTP effluents, both liquid and solid, when an anaerobic digestion step is included. The focus is placed on Clostridium spp. with the specific quantification of Clostridium perfringens, as typical bioindicator, and Clostridium difficile, as emerging pathogen not only confined into nosocomial infection...
December 15, 2016: Journal of Environmental Management
https://www.readbyqxmd.com/read/27631197/decay-kinetics-of-microbial-source-tracking-mst-markers-and-human-adenovirus-under-the-effects-of-sunlight-and-salinity
#3
Liang L, Goh S G, Gin K Y H
Artificial seawater and freshwater microcosms inoculated with raw sewage were set up to compare the persistence of microbial source tracking (MST) markers (i.e. Bacteroides thetaiotaomicron (B. theta), Methanobrevibacter smithii (M. smithii), human polyomaviruses JC and BK (HPyVs)) and human adenoviruses under different sunlight intensity and salinity. PMA pretreatment successfully eliminated the false-positive detection of dead bacterial cells in the model-development experiment. The results were then validated using real environmental matrices in microcosms inoculated with raw sewage...
September 12, 2016: Science of the Total Environment
https://www.readbyqxmd.com/read/27559429/complete-genome-sequence-of-thermophilic-bacillus-smithii-type-strain-dsm-4216-t
#4
Elleke F Bosma, Jasper J Koehorst, Sacha A F T van Hijum, Bernadet Renckens, Bastienne Vriesendorp, Antonius H P van de Weijer, Peter J Schaap, Willem M de Vos, John van der Oost, Richard van Kranenburg
Bacillus smithii is a facultatively anaerobic, thermophilic bacterium able to use a variety of sugars that can be derived from lignocellulosic feedstocks. Being genetically accessible, it is a potential new host for biotechnological production of green chemicals from renewable resources. We determined the complete genomic sequence of the B. smithii type strain DSM 4216(T), which consists of a 3,368,778 bp chromosome (GenBank accession number CP012024.1) and a 12,514 bp plasmid (GenBank accession number CP012025...
2016: Standards in Genomic Sciences
https://www.readbyqxmd.com/read/27458176/irritable-bowel-syndrome-particularly-the-constipation-predominant-form-involves-an-increase-in-methanobrevibacter-smithii-which-is-associated-with-higher-methane-production
#5
Ujjala Ghoshal, Ratnakar Shukla, Deepakshi Srivastava, Uday C Ghoshal
Background/Aims: Because Methanobrevibacter smithii produces methane, delaying gut transit, we evaluated M. smithii loads in irritable bowel syndrome (IBS) patients and healthy controls (HC). Methods: Quantitative real-time polymerase chain reaction for M. smithii was performed on the feces of 47 IBS patients (Rome III) and 30 HC. On the lactulose hydrogen breath test (LHBT, done for 25 IBS patients), a fasting methane result ≥10 ppm using 10 g of lactulose defined methane-producers...
July 27, 2016: Gut and Liver
https://www.readbyqxmd.com/read/27401817/dynamic-polarization-vision-in-mantis-shrimps
#6
Ilse M Daly, Martin J How, Julian C Partridge, Shelby E Temple, N Justin Marshall, Thomas W Cronin, Nicholas W Roberts
Gaze stabilization is an almost ubiquitous animal behaviour, one that is required to see the world clearly and without blur. Stomatopods, however, only fix their eyes on scenes or objects of interest occasionally. Almost uniquely among animals they explore their visual environment with a series pitch, yaw and torsional (roll) rotations of their eyes, where each eye may also move largely independently of the other. In this work, we demonstrate that the torsional rotations are used to actively enhance their ability to see the polarization of light...
2016: Nature Communications
https://www.readbyqxmd.com/read/27347377/lovastatin-lactone-may-improve-irritable-bowel-syndrome-with-constipation-ibs-c-by-inhibiting-enzymes-in-the-archaeal-methanogenesis-pathway
#7
Steven M Muskal, Joe Sliman, John Kokai-Kun, Mark Pimentel, Vince Wacher, Klaus Gottlieb
UNLABELLED: Methane produced by the methanoarchaeon Methanobrevibacter smithii ( M. smithii) has been linked to constipation, irritable bowel syndrome with constipation (IBS-C), and obesity. Lovastatin, which demonstrates a cholesterol-lowering effect by the inhibition of HMG-CoA reductase, may also have an anti-methanogenesis effect through direct inhibition of enzymes in the archaeal methanogenesis pathway. We conducted protein-ligand docking experiments to evaluate this possibility...
2016: F1000Research
https://www.readbyqxmd.com/read/27183876/increased-gut-redox-and-depletion-of-anaerobic-and-methanogenic-prokaryotes-in-severe-acute-malnutrition
#8
Matthieu Million, Maryam Tidjani Alou, Saber Khelaifia, Dipankar Bachar, Jean-Christophe Lagier, Niokhor Dione, Souleymane Brah, Perrine Hugon, Vincent Lombard, Fabrice Armougom, Julien Fromonot, Catherine Robert, Caroline Michelle, Aldiouma Diallo, Alexandre Fabre, Régis Guieu, Cheikh Sokhna, Bernard Henrissat, Philippe Parola, Didier Raoult
Severe acute malnutrition (SAM) is associated with inadequate diet, low levels of plasma antioxidants and gut microbiota alterations. The link between gut redox and microbial alterations, however, remains unexplored. By sequencing the gut microbiomes of 79 children of varying nutritional status from three centers in Senegal and Niger, we found a dramatic depletion of obligate anaerobes in malnutrition. This was confirmed in an individual patient data meta-analysis including 107 cases and 77 controls from 5 different African and Asian countries...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27141986/influence-of-periparturient-and-postpartum-diets-on-rumen-methanogen-communities-in-three-breeds-of-primiparous-dairy-cows
#9
Laura M Cersosimo, Melissa L Bainbridge, Jana Kraft, André-Denis G Wright
BACKGROUND: Enteric methane from rumen methanogens is responsible for 25.9 % of total methane emissions in the United States. Rumen methanogens also contribute to decreased animal feed efficiency. For methane mitigation strategies to be successful, it is important to establish which factors influence the rumen methanogen community and rumen volatile fatty acids (VFA). In the present study, we used next-generation sequencing to determine if dairy breed and/or days in milk (DIM) (high-fiber periparturient versus high-starch postpartum diets) affect the rumen environment and methanogen community of primiparous Holstein, Jersey, and Holstein-Jersey crossbreeds...
2016: BMC Microbiology
https://www.readbyqxmd.com/read/27010812/aerobic-culture-of-methanogenic-archaea-without-an-external-source-of-hydrogen
#10
S Khelaifia, J-C Lagier, V D Nkamga, E Guilhot, M Drancourt, D Raoult
Culturing methanogenic archaea is fastidious, expensive, and requires an external source of hydrogen and carbon dioxide. Until now, these microorganisms have only been cultivated under strictly anaerobic conditions. We previously developed a single versatile culture medium containing sugars and anti-oxydants for cultivating all human known methanogens. Performing aerobic cultures in the presence of Bacteroides thetaiotaomicron, which produces hydrogen, allows for cultivation of Methanobrevibacter smithii which itself produces methane...
June 2016: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/26833719/metabolic-effects-of-eradicating-breath-methane-using-antibiotics-in-prediabetic-subjects-with-obesity
#11
Ruchi Mathur, Kathleen S Chua, Mindy Mamelak, Walter Morales, Gillian M Barlow, Rita Thomas, Darko Stefanovski, Stacy Weitsman, Zachary Marsh, Richard N Bergman, Mark Pimentel
OBJECTIVE: Methanogens colonizing the human gut produce methane and influence host metabolism. This study examined metabolic parameters in methane-producing subjects before and after antibiotic treatment. METHODS: Eleven prediabetic methane-positive subjects (9F, 2M) with obesity (BMI 35.17 ± 7.71 kg/m(2) ) aged 47 ± 9 years were recruited. Subjects underwent breath testing, symptom questionnaire, oral glucose tolerance test (OGTT), lipid profile, and stool Methanobrevibacter smithii levels, gastric transit, and energy utilization analyses...
March 2016: Obesity
https://www.readbyqxmd.com/read/26572011/-spatial-point-pattern-analysis-of-abies-georgei-var-smithii-in-forest-of-sygera-mountains-in-southeast-tibet-china
#12
Chuan-qi Xie, Min-xia Tian, Zhong-rui Zhao, Wei-lie Zheng, Guo-yan Wang
In this study, based on a 4 hm2 stem-mapping plot, we analyzed spatial distributions of Abies georgei var. smithii, the dominant species in forest of Sygera Mountains in southeast Tibet, China. Pair-correlation function was used to characterize univariate spatial point patterns of three size classes of the population and bivariate spatial patterns between those and different sizes of dead wood. A. georgei var. smithii population was characterized by reverse J-shaped DBH distribution, indicating an increasing population...
June 2015: Ying Yong Sheng Tai Xue Bao, the Journal of Applied Ecology
https://www.readbyqxmd.com/read/26559904/review-article-inhibition-of-methanogenic-archaea-by-statins-as-a-targeted-management-strategy-for-constipation-and-related-disorders
#13
REVIEW
K Gottlieb, V Wacher, J Sliman, M Pimentel
BACKGROUND: Observational studies show a strong association between delayed intestinal transit and the production of methane. Experimental data suggest a direct inhibitory activity of methane on the colonic and ileal smooth muscle and a possible role for methane as a gasotransmitter. Archaea are the only confirmed biological sources of methane in nature and Methanobrevibacter smithii is the predominant methanogen in the human intestine. AIM: To review the biosynthesis and composition of archaeal cell membranes, archaeal methanogenesis and the mechanism of action of statins in this context...
January 2016: Alimentary Pharmacology & Therapeutics
https://www.readbyqxmd.com/read/26551842/correlation-between-body-mass-index-and-faecal-microbiota-from-children
#14
A Ignacio, M R Fernandes, V A A Rodrigues, F C Groppo, A L Cardoso, M J Avila-Campos, V Nakano
Childhood obesity is an increasing problem at the global level and considered as a risk factor for obesity development and the associated co-morbidities in adult life. In this study, the occurrence of Bacteroides fragilis group, Clostridium spp., Bifidobacterium spp. and Escherichia coli in 84 faecal samples from 30 obese, 24 overweight and 30 lean children was verified by culture technique and quantitative determination by quantitative PCR. In addition, Lactobacillus spp. and Methanobrevibacter smithii were also analysed...
March 2016: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/26524691/gut-colonization-with-methanobrevibacter-smithii-is-associated-with-childhood-weight-development
#15
Catherine A Mbakwa, John Penders, Paul H Savelkoul, Carel Thijs, Pieter C Dagnelie, Monique Mommers, Ilja C W Arts
OBJECTIVE: To prospectively investigate the presence and counts of archaea in feces of 472 children in association with weight development from 6 to 10 years of age. METHODS: Within the KOALA Birth Cohort Study, a single fecal sample from each child was analyzed by quantitative polymerase chain reaction to quantify archaea (Methanobrevibacter smithii, Methanosphera stadtmanae). Anthropometric outcomes (overweight [body mass index {BMI} ≥ 85th percentile], age- and sex-standardized BMI, weight, and height z-scores) were repeatedly measured at ages (mean ± SD) of 6...
December 2015: Obesity
https://www.readbyqxmd.com/read/26471259/armored-geckos-a-histological-investigation-of-osteoderm-development-in-tarentola-phyllodactylidae-and-gekko-gekkonidae-with-comments-on-their-regeneration-and-inferred-function
#16
M K Vickaryous, G Meldrum, A P Russell
Osteoderms are bone-rich organs found in the dermis of many scleroglossan lizards sensu lato, but are only known for two genera of gekkotans (geckos): Tarentola and Gekko. Here, we investigate their sequence of appearance, mode of development, structural diversity and ability to regenerate following tail loss. Osteoderms were present in all species of Tarentola sampled (Tarentola annularis, T. mauritanica, T. americana, T. crombei, T. chazaliae) as well as Gekko gecko, but not G. smithii. Gekkotan osteoderms first appear within the integument dorsal to the frontal bone or within the supraocular scales...
November 2015: Journal of Morphology
https://www.readbyqxmd.com/read/26361472/reaction-of-five-non-cereal-grasses-to-five-races-and-two-host-selective-toxins-of-pyrenophora-tritici-repentis
#17
Shaukat Ali, M A C Langham
Alternative hosts increase the difficulty of disease management in crops because these alternate hosts provide additional sources of primary inoculum or refuges for diversity in the pathogen gene pool. Agropyron cristatum (crested wheatgrass), Bromus inermis (smooth bromegrass), Pascopyrum smithii (western wheatgrass), Stipa viridula (green needlegrass), and Thinopyrum intermedium (intermediate wheatgrass), commonly identified in range, prairie, verge, and soil reclamation habitats, serve as additional hosts for Pyrenophora tritici-repentis, the cause of tan spot in wheat (Triticum aestivum L...
September 2015: Plant Pathology Journal
https://www.readbyqxmd.com/read/26248595/armored-geckos-a-histological-investigation-of-osteoderm-development-in-tarentola-phyllodactylidae-and-gekko-gekkonidae-with-comments-on-their-regeneration-and-inferred-function
#18
M K Vickaryous, G Meldrum, A P Russell
Osteoderms are bone-rich organs found in the dermis of many scleroglossan lizards sensu lato, but are only known for two genera of gekkotans (geckos): Tarentola and Gekko. Here, we investigate their sequence of appearance, mode of development, structural diversity and ability to regenerate following tail loss. Osteoderms were present in all species of Tarentola sampled (Tarentola annularis, T. mauritanica, T. americana, T. crombei, T. chazaliae) as well as Gekko gecko, but not G. smithii. Gekkotan osteoderms first appear within the integument dorsal to the frontal bone or within the supraocular scales...
November 2015: Journal of Morphology
https://www.readbyqxmd.com/read/26148486/establishment-of-markerless-gene-deletion-tools-in-thermophilic-bacillus-smithii-and-construction-of-multiple-mutant-strains
#19
Elleke F Bosma, Antonius H P van de Weijer, Laurens van der Vlist, Willem M de Vos, John van der Oost, Richard van Kranenburg
BACKGROUND: Microbial conversion of biomass to fuels or chemicals is an attractive alternative for fossil-based fuels and chemicals. Thermophilic microorganisms have several operational advantages as a production host over mesophilic organisms, such as low cooling costs, reduced contamination risks and a process temperature matching that of commercial hydrolytic enzymes, enabling simultaneous saccharification and fermentation at higher efficiencies and with less enzymes. However, genetic tools for biotechnologically relevant thermophiles are still in their infancy...
2015: Microbial Cell Factories
https://www.readbyqxmd.com/read/25903267/archaeal-microbiota-population-in-piglet-feces-shifts-in-response-to-weaning-methanobrevibacter-smithii-is-replaced-with-methanobrevibacter-boviskoreani
#20
Sara Federici, Francesco Miragoli, Vincenza Pisacane, Annalisa Rebecchi, Lorenzo Morelli, Maria Luisa Callegari
Methanogens commonly inhabit swine intestine. We analyzed the gut archaeal population by extracting DNA from the feces of nine piglets. We performed PCR to target the V6-V8 region of the 16S rRNA gene. Subsequent denaturing gradient gel electrophoresis (DGGE) revealed the presence of Methanobrevibacter boviskoreani, which has not previously been identified in pigs. We confirmed these data with a PCR-DGGE analysis of the mcrA gene, and subsequent sequencing. At 63 days old, the only band in fecal samples corresponded to M...
May 2015: FEMS Microbiology Letters
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