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Langendorff heart

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https://www.readbyqxmd.com/read/28942508/role-of-inducible-nitric-oxide-synthase-in-myocardial-ischemia-reperfusion-injury-in-sleep-deprived-rats
#1
Sajad Jeddi, Asghar Ghasemi, Alireza Asgari, Amir Nezami-Asl
INTRODUCTION: REM sleep deprivation (SD) decreases tolerance of the rat heart to ischemia-reperfusion (IR) injury; the underlying mechanisms, however, are unknown. This study aimed at determining whether changes in iNOS, Bax, and Bcl-2 gene expression are involved in this detrimental effect. METHOD: SD was induced by flowerpot technique for a period of 4 days. This method is simple and able to induce sleep fragmentation which occurs as one of the sleep disorder symptoms in clinical conditions...
September 23, 2017: Sleep & Breathing, Schlaf & Atmung
https://www.readbyqxmd.com/read/28939647/intra-cardiac-light-catheter-for-transmural-absorbance-spectroscopy-of-perfused-myocardium-measurement-of-myoglobin-oxygenation-and-mitochondria-redox-state
#2
Armel N Femnou, Sarah Kuzmiak-Glancy, Raul Covian, Abigail V Giles, Matthew W Kay, Robert S Balaban
Absorbance spectroscopy of intrinsic cardiac chromophores provides non-destructive assessment of cytosolic oxygenation and mitochondria redox state. Isolated perfused heart spectroscopy is usually conducted by collecting reflected light from the heart surface, which represents a combination of surface scattering events and light that traversed portions of the myocardium. Reflectance spectroscopy with complex surface scattering effects in the beating heart leads to difficulty in quantitating chromophore absorbance...
September 22, 2017: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/28934223/ursodeoxycholic-acid-prevents-ventricular-conduction-slowing-and-arrhythmia-by-restoring-t-type-calcium-current-in-fetuses-during-cholestasis
#3
Oladipupo Adeyemi, Anita Alvarez-Laviada, Francisca Schultz, Effendi Ibrahim, Michael Trauner, Catherine Williamson, Alexey V Glukhov, Julia Gorelik
BACKGROUND: Increased maternal serum bile acid concentrations in intrahepatic cholestasis of pregnancy (ICP) are associated with fetal cardiac arrhythmias. Ursodeoxycholic acid (UDCA) has been shown to demonstrate anti-arrhythmic properties via preventing ICP-associated cardiac conduction slowing and development of reentrant arrhythmias, although the cellular mechanism is still being elucidated. METHODS: High-resolution fluorescent optical mapping of electrical activity and electrocardiogram measurements were used to characterize effects of UDCA on one-day-old neonatal and adult female Langendorff-perfused rat hearts...
2017: PloS One
https://www.readbyqxmd.com/read/28929376/divergent-coronary-flow-responses-to-uridine-adenosine-tetraphosphate-in-atherosclerotic-apoe-knockout-mice
#4
Bunyen Teng, Hicham Labazi, Changyan Sun, Yan Yang, Xiaorong Zeng, S Jamal Mustafa, Zhichao Zhou
Uridine adenosine tetraphosphate (Up4A) exerts potent relaxation in porcine coronary arteries that is reduced following myocardial infarction, suggesting a crucial role for Up4A in the regulation of coronary flow (CF) in cardiovascular disorders. We evaluated the vasoactive effects of Up4A on CF in atherosclerosis using ApoE knockout (KO) mice ex vivo and in vivo. Functional studies were conducted in isolated mouse hearts using the Langendorff technique. Immunofluorescence was performed to assess purinergic P2X1 receptor (P2X1R) expression in isolated mouse coronary arteries...
September 20, 2017: Purinergic Signalling
https://www.readbyqxmd.com/read/28920462/involvement-of-atrial-natriuretic-peptide-in-abrogated-cardioprotective-effect-of-ischemic-preconditioning-in-ovariectomized-rat-heart
#5
V K Vishwakarma, A Goyal, J K Gupta, P K Upadhyay, H N Yadav
BACKGROUND: Nitric oxide (NO) is an effective mediator of ischemic preconditioning (IPC)-induced cardioprotection. Atrial natriuretic peptide (ANP) is downregulated after ovariectomy, which results in reduction in the level of NO. The present study deals with the investigation of the role of ANP in abrogated cardioprotective effect of IPC in the ovariectomized rat heart. METHODS: Heart was isolated from ovariectomized rat and mounted on Langendorff's apparatus, subjected to 30 min of ischemia and 120 min of reperfusion...
January 1, 2017: Human & Experimental Toxicology
https://www.readbyqxmd.com/read/28920042/mitochondrial-atp-sensitive-potassium-channels-play-a-role-in-reducing-both-myocardial-infarction-and-reperfusion-arrhythmia-in-remote-ischemic-preconditioned-hearts
#6
Young-Ho Jang, June-Hong Kim, Yong-Cheol Lee
BACKGROUND: Mitochondrial ATP-sensitive potassium (mKATP) channels play a role in reperfusion arrhythmias (RAs) in ischemia-reperfusion (I/R) injury. Evidence suggests that remote ischemic preconditioning (RIPC) reduces RAs, however not much is known on the mechanistic role of mKATP in RIPC. We evaluated whether mKATP channels are associated with reducing arrhythmia and infarct size in RIPC. METHODS: Isolated rat hearts received 30 minutes of regional ischemia followed by 2 hours of reperfusion through the Langendorff perfusion system...
February 2017: Anesthesiology and Pain Medicine
https://www.readbyqxmd.com/read/28919427/age-dependent-atrial-arrhythmic-phenotype-secondary-to-mitochondrial-dysfunction-in-pgc-1%C3%AE-deficient-murine-hearts
#7
Haseeb Valli, Shiraz Ahmad, Karan R Chadda, Ali B A K Al-Hadithi, Andrew A Grace, Kamalan Jeevaratnam, Christopher L-H Huang
INTRODUCTION: Ageing and several age-related chronic conditions including obesity, insulin resistance and hypertension are associated with mitochondrial dysfunction and represent independent risk factors for atrial fibrillation (AF). MATERIALS AND METHODS: Atrial arrhythmogenesis was investigated in Langendorff-perfused young (3-4 month) and aged (>12 month), wild type (WT) and peroxisome proliferator activated receptor-γ coactivator-1β deficient (Pgc-1β(-/-)) murine hearts modeling age-dependent chronic mitochondrial dysfunction during regular pacing and programmed electrical stimulation (PES)...
September 14, 2017: Mechanisms of Ageing and Development
https://www.readbyqxmd.com/read/28914648/dexmedetomidine-maintains-its-direct-cardioprotective-effect-against-ischemia-reperfusion-injury-in-hypertensive-hypertrophied-myocardium
#8
Yusuke Yoshikawa, Naoyuki Hirata, Ryoichi Kawaguchi, Yasuyuki Tokinaga, Michiaki Yamakage
BACKGROUND: Dexmedetomidine (DEX) has a direct cardioprotective effect against ischemia/reperfusion injury through endothelial nitric oxide synthase (eNOS) phosphorylation via α2-adrenoreceptor (α2-AR). By using spontaneously hypertensive rat (SHR) and Wistar-Kyoto (WKY) rat models, the cardioprotective effect of DEX in hypertrophied myocardium and the differential characteristics of cardiac α2-AR and the I1 imidazoline receptor (I1R) were examined. METHODS: Langendorff-perfused rat hearts underwent 40 minutes of global ischemia followed by 120 minutes of reperfusion in the presence or absence of DEX before ischemia...
September 12, 2017: Anesthesia and Analgesia
https://www.readbyqxmd.com/read/28910959/-ischemia-reperfusion-injury-study-in-isolated-mouse-hearts-using-a-pressure-volume-curve
#9
J B Jiang, Z W Huamei, Gourdie Robert G, Zhiwei Zhang
Objective: To establish and assess the feasibility and sensitivity of left ventricular elasticity, compliance and stiffness for study of ischemia/reperfusion injury in an isolated mouse heart model utilizing the pressure-volume curve framework. Methods: An isolated, balloon-in-ventricle, isovolumically contracting, crystalloid-perfused Langendorff heart preparation was set up from 15 male C57/6BL mice aged 12-14 weeks. End-systolic pressure-volume relationship (ESPVR) and end-diastolic pressure-volume relationship (EDPVR) were obtained by measuring left ventricular (LV) systolic pressure (LVSP) and diastolic pressure under different balloon volumes...
September 12, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/28901167/effect-of-ischemic-postconditioning-on-myocardial-function-and-infarct-size-following-reperfusion-injury-in-diabetic-rats-pretreated-with-vildagliptin
#10
Goltaj Bayrami, Pouran Karimi, Fariba Agha-Hosseini, Saeid Feyzizadeh, Reza Badalzadeh
BACKGROUND: Cardioprotective actions of ischemic postconditioning (IPostC) against ischemia/reperfusion (I/R) injury are abolished in diabetic hearts. This study has investigated the combined effects of IPostC and vildagliptin (Vilda) on myocardial function and infarct size (IS) against I/R injury in diabetic myocardium. METHODS: Diabetes was induced by a high-fat diet/low dose of streptozotocin (35 mg/kg; intraperitoneally) in Wistar rats (200-250 g) and lasted for 12 weeks...
January 1, 2017: Journal of Cardiovascular Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/28893911/molecular-and-functional-characterization-of-the-endothelial-atp-sensitive-potassium-channel
#11
Qadeer Aziz, Yiwen Li, Naomi Anderson, Leona Ojake, Elena Tsisanova, Andrew Tinker
ATP-sensitive potassium (KATP) channels are widely expressed in the cardiovascular system where they regulate a range of biological activities by linking cellular metabolism with membrane excitability. KATP channels in vascular smooth muscle have a well-defined role in regulating vascular tone. KATP channels are also thought to be expressed in vascular endothelial cells (ECs), but their presence and function in this context is less clear. As a result, we aimed to investigate the molecular composition and physiological role of endothelial KATP channels...
September 11, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28887130/gender-specific-impairment-of-in-vitro-sinoatrial-node-chronotropic-responses-and-of-myocardial-ischemia-tolerance-in-rats-exposed-prenatally-to-betamethasone
#12
L R A Kiguti, C S Borges, A Mueller, K P Silva, C M Polo, J L Rosa, P V Silva, G Missassi, L Valencise, W G Kempinas, A S Pupo
Excessive fetal glucocorticoid exposure has been linked to increased susceptibility to hypertension and cardiac diseases in the adult life, a process called fetal programming. The cardiac contribution to the hypertensive phenotype of glucocorticoid-programmed progeny is less known, therefore, we investigated in vitro cardiac functional parameters from rats exposed in utero to betamethasone. Pregnant Wistar rats received vehicle (VEH) or betamethasone (BET, 0.1mg/kg, i.m.) at gestational days 12, 13, 18 and 19...
September 5, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/28877068/mc225-a-novel-probe-for-p-glycoprotein-pet-imaging-at-the-blood-brain-barrier-in-vitro-cardiovascular-safety-evaluation
#13
Fabio Fusi, Miriam Durante, Beatrice Gorelli, Maria Grazia Perrone, Colabufo Nicola Antonio, Simona Saponara
The P-glycoprotein (P-gp) substrate MC225, at concentrations ≤ 10 nM, is a valuable radiotracer for positron emission tomography (PET) imaging of P-gp function in rats and mice.The aim of this study was to evaluate its potential toxic hazard towards the cardiovascular system through an in-depth analysis of its effects on rat aorta rings, on CaV1.2 channel current (ICa1.2) of A7r5 cells and on Langendorff perfused rat heart.In aortic rings, MC225 relaxed phenylephrine-induced contraction in a concentration-dependent and endothelium-independent manner, with an IC50 value of about 1 µM...
September 1, 2017: Journal of Cardiovascular Pharmacology
https://www.readbyqxmd.com/read/28863155/electrophysiological-characteristics-of-pressure-overload-induced-cardiac-hypertrophy-and-its-influence-on-ventricular-arrhythmias
#14
Xiaowei Chen, Mu Qin, Weifeng Jiang, Yu Zhang, Xu Liu
OBJECTIVE: To explore the cardiac electrophysiological characteristics of cardiac hypertrophy and its influence on the occurrence of ventricular tachyarrhythmias. METHODS: Adult C57BL6 mice were randomly divided into a surgery group and a control group. Thoracic aortic constriction was performed on mice in the surgery group, and cardiac anatomical and ultrasonic evaluations were performed to confirm the success of the cardiac hypertrophy model 4 weeks after the operation...
2017: PloS One
https://www.readbyqxmd.com/read/28859848/chronic-in-vivo-nitric-oxide-deficiency-impairs-cardiac-functional-recovery-after-ischemia-in-female-but-not-male-mice
#15
Laura A Bienvenu, James Morgan, Melissa E Reichelt, Lea M D Delbridge, Morag J Young
Nitric oxide (NO) is an important regulator of cardiac function and plays a key role in ischemic cardioprotection. The role of chronic NO deficiency in coordinating ischemic vulnerability in female myocardium has not been established. The aim of this study was to determine the influence of chronic in vivo NO synthase inhibition in modulating ex vivo ischemia-reperfusion responses in female hearts (relative to males). Mice were subjected to l-NAME (l-N(G)-Nitroarginine-methyl-ester) treatment in vivo for 8weeks...
August 30, 2017: Journal of Molecular and Cellular Cardiology
https://www.readbyqxmd.com/read/28852440/the-cardioprotective-effect-of-vanillic-acid-on-hemodynamic-parameters-malondialdehyde-and-infarct-size-in-ischemia-reperfusion-isolated-rat-heart-exposed-to-pm10
#16
Esmat Radmanesh, Mahin Dianat, Mohammad Badavi, Gholamreza Goudarzi, Seyyed Ali Mard
OBJECTIVES: Particulate matter (PM) exposure can promote cardiac ischemia and myocardial damage. The effects of PM10 on hemodynamic parameters, lipid peroxidation, and infarct size induced by ischemia-reperfusion injury and the protective effects of vanillic acid (VA) in isolated rat heart were investigated. MATERIALS AND METHODS: Eighty male Wistar rats (250-300 g) were divided into 8 groups (n=10): Control, Sham, VAc, VA, PMa (0.5 mg/kg PM, intratracheal instillation), PMb (2...
July 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28842915/roles-of-impaired-intracellular-calcium-cycling-in-arrhythmogenicity-of-diabetic-mouse-model
#17
Chung-Chuan Chou, Chien-Te Ho, Hui-Ling Lee, Yen Chu, Tzung-Hai Yen, Ming-Shien Wen, Shien-Fong Lin, Cheng-Hung Lee, Po-Cheng Chang
BACKGROUND: Diabetes mellitus is associated an increased risk of ventricular arrhythmias (VA), but the underlying electrophysiological mechanisms are not fully explored. This study was aimed to test whether dynamic factors and Cai handling play roles in arrhythmogenesis of a diabetic animal model. METHODS: We used 26 db/db type 2 diabetes mice and 28 control mice in this study. VA inducibility was evaluated in vivo under isoflurane general anesthesia. The intracellular Ca(2+) (Cai ) and membrane voltage (Vm ) signals of the Langendorff-perfused mouse hearts were simultaneously recorded using the optical mapping technique...
August 26, 2017: Pacing and Clinical Electrophysiology: PACE
https://www.readbyqxmd.com/read/28827743/mir-125a-mir-139-and-mir-324-contribute-to-urocortin-protection-against-myocardial-ischemia-reperfusion-injury
#18
Ignacio Díaz, Eva Calderón-Sánchez, Raquel Del Toro, Javier Ávila-Médina, Eva Sánchez de Rojas-de Pedro, Alejandro Domínguez-Rodríguez, Juan Antonio Rosado, Abdelkrim Hmadcha, Antonio Ordóñez, Tarik Smani
Urocortin 1 and 2 (Ucn-1 and Ucn-2) have established protective actions against myocardial ischemia-reperfusion (I/R) injuries. However, little is known about their role in posttranscriptional regulation in the process of cardioprotection. Herein, we investigated whether microRNAs play a role in urocortin-induced cardioprotection. Administration of Ucn-1 and Ucn-2 at the beginning of reperfusion significantly restored cardiac function, as evidenced ex vivo in Langendorff-perfused rat hearts and in vivo in rat subjected to I/R...
August 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821956/effects-of-ageing-on-pro-arrhythmic-ventricular-phenotypes-in-incrementally-paced-murine-pgc-1%C3%AE-hearts
#19
Shiraz Ahmad, Haseeb Valli, Charlotte E Edling, Andrew A Grace, Kamalan Jeevaratnam, Christopher L-H Huang
A range of chronic clinical conditions accompany cardiomyocyte energetic dysfunction and constitute independent risk factors for cardiac arrhythmia. We investigated pro-arrhythmic and arrhythmic phenotypes in energetically deficient C57BL mice with genetic ablation of the mitochondrial promoter peroxisome proliferator-activated receptor-γ coactivator-1β (Pgc-1β), a known model of ventricular arrhythmia. Pro-arrhythmic and cellular action potential (AP) characteristics were compared in intact Langendorff-perfused hearts from young (12-16 week) and aged (> 52 week), wild-type (WT) and Pgc-1β (-/-) mice...
August 18, 2017: Pflügers Archiv: European Journal of Physiology
https://www.readbyqxmd.com/read/28811127/attenuation-of-sunitinib-induced-cardiotoxicity-through-the-a3-adenosine-receptor-activation
#20
Hardip Sandhu, Samantha Cooper, Afthab Hussain, Christopher Mee, Helen Maddock
Sunitinib is an anti-cancer tyrosine kinase inhibitor associated with severe cardiotoxic adverse effects. Using rat Langendorff heart model and human acute myeloid leukaemia 60 (HL60) cell line we detected the involvement of protein kinase C (PKC) α during Sunitinib-induced cardiotoxicity and the effect of Sunitinib on cancer progression. The cardioprotective and anti-cancer properties of the A3 adenosine receptor agonist 2-chloro-N6-(3-iodobenzyl)-adenosine-5'-N-methyluronamide (IB-MECA) were investigated...
August 12, 2017: European Journal of Pharmacology
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