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Brompoj Prutthiwanasan, Leena Suntornsuk
A simple micellar electrokinetic chromatography (MEKC), using cetyltrimethyl ammonium bromide (CTAB) as micelles, for the determination of ciprofloxacin (CIP), enrofloxacin (ENR), norfloxacin (NOR) and ofloxacin (OFL) residues in surface water was developed. Peak Master was used for predicting amounts of analyte ionic forms to reduce numbers of tedious experiments in optimizing the analyte capacity factors. A baseline separation (Rs  > 2.8) of the analytes was achieved in 7 min using 15 mM sodium dihydrogen phosphate (pH 6...
June 18, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Xiaona Dong, Yiwei Zhang, Zhiyun Meng, Xiaoxia Zhu, Hui Gan, Ruolan Gu, Zhuona Wu, Jian Li, Ying Zheng, Baofeng Yang, Guifang Dou
HYD-PEP06 is a novel RGD-modified Endostar mimetic peptide with 30 amino acids that is intended to suppress the formation of neoplasm vessels. This assay was developed and validated to monitor the level of the peptide HYD-PEP06 in rat blood, using liquid chromatography tandem mass spectrometry (LC-MS/MS). HYD-PEP10, another peptide similar to the analyte, was used as an internal standard (IS). A triple quadrupole mass spectrometry in Multiple Reaction Monitoring (MRM) mode and an electrospray interface (ESI) in the positive mode were used for MS analysis...
May 29, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Roghayeh Mousavi-Khatir, Saeed Talebian, Nima Toosizadeh, Gholam Reaza Olyaei, Nader Maroufi
The highly complex proprioceptive system provides neuromuscular control of the mobile cervical spine. Static neck flexion can induce the elongation of posterior tissues and altered afferent input from the mechanoreceptors. The purpose of this study was to examine the effect of prolonged static neck flexion on neck proprioception and anticipatory postural adjustments. Thirty-eight healthy participants (20 females and 18 males) between the ages of 20-35 years with no history of neck, low back, and shoulder pain enrolled in this study...
April 24, 2018: Journal of Electromyography and Kinesiology
Boman Chen, Min Cheng, Siqi Hong, Shuang Liao, Jiannan Ma, Tingsong Li, Li Jiang
PURPOSE: To assess the clinical outcome and evolution of recurrent afebrile seizures in children initially diagnosed with benign convulsions associated with mild gastroenteritis (CwG). METHODS: We reviewed and analyzed the medical records of 37 patients who were diagnosed as CwG at onset, followed by recurrent afebrile seizures and followed up for at least 24 months. RESULTS: The follow-up period ranged from 2 to 7 years (median, 40.1 months)...
May 30, 2018: Seizure: the Journal of the British Epilepsy Association
Utsav Shashvatt, Josh Benoit, Hannah Aris, Lee Blaney
Phosphorus recovery from industrialized poultry operations is necessary to ensure sustainable waste management and resource consumption. To realize these goals, an innovative, two-stage process chemistry has been developed to extract nutrients from poultry litter and recover value-added products. Over 75% phosphorus extraction was achieved by bubbling carbon dioxide into poultry litter slurries and adding strong acid to reach pH 4.5-5.5. After separating the nutrient-deficient poultry litter solids and the nutrient-rich liquid, the extract pH was increased through aeration and strong base addition...
June 18, 2018: Water Research
Samah S Abbas, Hesham M Mahmoud, Mona F Schaalan, Hanan S El-Abhar
BACKGROUND: The current study is focusing on the role of brain natriuretic peptide (BNP), a substrate of dipeptidyl peptidase-4 (DPP-4) enzyme, and its signaling survival pathway in the cardioprotective mechanism of sitagliptin, a DPP-4 inhibitor. METHODS: Male Wistar rats were randomized into 7 groups, sham, I/R, KT-5823 (selective protein kinase (PK) G inhibitor), 5-HD (selective mito-KATP channel blocker), sitagliptin (300 mg/kg, po), sitagliptin + KT-5823, and sitagliptin + 5-HD...
February 7, 2018: Pharmacological Reports: PR
Fan Wang, Junyong Mo, Aimin Huang, Min Zhang, Lin Ma
Polyethyleneimine (PEI) has long been considered as "golden standard" for polymeric gene delivery carrier, however also induces cytotoxicity. To make a further insight into the molecular basis of PEI cytotoxicity, fluorescence, absorption and circular dichroism spectroscopy were conducted to investigate the influence of PEI (average molecular weight 25,000 and 1800 Da) on the conformation of pig heart lactate dehydrogenase (LDH) and its catalytic efficiency. Zeta-potential measurement and isothermal titration calorimetry were used to reveal the interaction between PEI and LDH...
June 15, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Xu Jianping, Wei Kunhua, Zhang Guojun, Lei Lujing, Yang Dawei, Wang Wenle, Han Qiheng, Xia Yuan, Bi Yaqiong, Yang Min, Li Minhui
ETHNOPHARMACOLOGICAL RELEVANCE: The genus Salvia is one of the largest genera of the Lamiaceae family. In China, about 40 Salvia species have been used as medicinal plants for treatment of various diseases, specifically hepatic and renal diseases and those of the cardiovascular and immune systems. AIM OF THIS REVIEW: This review aims to provide systematically organized information on the ethnopharmacology, phytochemistry, pharmacology, and toxicology of medicinal Salvia species in China to support their therapeutic potential in the treatment of human diseases...
June 20, 2018: Journal of Ethnopharmacology
Li-Ling Yang, Ming-Yue Zhan, Yu-Li Zhuo, Yue-Min Pan, Yang Xu, Xiu-Hong Zhou, Pei-Jin Yang, Hong-Li Liu, Zi-Hao Liang, Xiao-Dan Huang, Xiao-Qiang Yu, Xiang-Jun Rao
Antimicrobial peptides (AMPs) are produced by the stimulated humoral immune system. Most mature AMPs contain less than 50 amino acid residues. Some of them are generated from proproteins upon microbial challenges. Here, we report the antimicrobial activities of a proline-rich proprotein, named SlLebocin1 (SlLeb1), from the tobacco cutworm Spodoptera litura. SlLebocin1 cDNA contains a 477-bp open reading frame (ORF). It is mainly expressed in hemocytes and the midgut in naïve larvae. The transcript level was significantly induced in hemocytes but repressed in the midgut and fat body by bacterial challenges...
June 20, 2018: Developmental and Comparative Immunology
Shijing Wu, Lantian Gao, Changshui Chen, Jun Li, Sailing He
Functional near-infrared spectroscopy (fNIRS) was used to investigate the stability of resting state functional connectivity (RSFC) in the prefrontal cortex. In a longitudinal study for investigating the stability of RSFC with time, we recruited 6 healthy adult subjects to undergo a 10-min resting state fNIRS scan once per day for 7 consecutive days. In a cross-sectional study, 62 healthy subjects underwent a single 10-min RSFC measurement. Three regions-of-interest (ROIs) were studied, the superior frontal gyrus (SFG), the middle frontal gyrus (MFG), and the inferior frontal gyrus (IFG)...
June 20, 2018: Neuroscience Letters
Jie-Li Lv, Lai-Bin Zhang, Li-Min Guo
Four new dimeric phthalides, angesinenolides C-F (1-4), along with three known ones, were isolated from the roots of Angelica sinensis. Their structures were determined by means of HRMS and NMR experiments. The structures of compounds 1 and 3 were confirmed using X-ray crystallographic data. All isolated compounds were tested for activities on the inhibition of COX-2 enzyme in vitro. Compounds 1-6 exhibited inhibitory activity against COX-2 with IC50 values ranging from 29.32 ± 0.07 to 137.91 ± 0...
June 20, 2018: Fitoterapia
Ramadevi M, Deepesh Panwar, Juby Elsa Joseph, Gaurav Singh Kaira, Mukesh Kapoor
A hitherto unknown low molecular weight form of α-galactosidase (VM-αGal) from germinating black gram (Vigna mungo) seeds was purified to homogeneity (432 U/mg specific activity, 1542-fold purification) using ion-exchange (DEAE-cellulose, CM-sepharose) and affinity (Con-A Sepharose 4B) chromatography. VM-αGal appeared as a single band ~ 45 kDa on SDS-PAGE and showed optimal activity at pH 5 and 55 °C. Hg2+ and SDS completely inhibited VM-αGal activity. The Km, Vmax and catalytic efficiency (kcat /Km ) of VM-αGal for pNPG and raffinose was found to be 0...
June 20, 2018: International Journal of Biological Macromolecules
Kazem Zibara, Asad Zeidan, Khalil Mallah, Nouhad Kassem, Ali Awwad, Frédéric Mazurier, Bassam Badran, Nabil El-Zein
PACAP has opposing roles ranging from activation to inhibition of tumor growth and PACAP agonists/antagonists could be used in tumor therapy. In this study, the effect of PACAP stimulation on signaling pathways was investigated in MCF-7 human adenocarcinoma breast cancer cells. Results showed that MCF-7 cells express VPAC1 and VPAC2, but not PAC1, receptors. In addition, PACAP increased the phosphorylation levels of STAT1, Src and Raf within seconds, confirming their involvement in early stages of PACAP signaling whereas maximal phosphorylation of AKT, ERK and p38 was reached 10 to 20 min later...
June 20, 2018: Cellular Signalling
Min Li, Yuetao Wen, Rongrong Zhang, Fei Xie, Gang Zhang, Xinyue Qin
BACKGROUND: Repulsive guidance molecule A (RGMa) is implicated in focal cerebral ischemia-reperfusion (I/R) injury, but its mechanisms are still largely unknown. This work focused on the effects of RGMa on the blood-brain barrier (BBB) after focal cerebral I/R injury. METHODS: Sprague-Dawley (SD) rats were randomly divided into four groups: sham, middle cerebral artery occlusion (MCAO)/reperfusion (I/R), MCAO/reperfusion administered recombinant adenovirus expressing sh-con (I/R + sh-con) and MCAO/reperfusion administered recombinant adenovirus expressing sh-RGMa (I/R + sh-RGMa) groups...
June 20, 2018: Brain Research Bulletin
Changhwan Ahn, Hong-Seok Kang, Jae-Hwan Lee, Eui-Ju Hong, Eui-Man Jung, Yeong-Min Yoo, Eui-Bae Jeung
In pancreatic β cells, which produce and secrete insulin, Ca2+ signals contribute to insulin production and secretion. Bisphenol A (BPA) and octylphenol (OP) are reported to increase plasma insulin levels and insulin transcription factors, but regulation of plasma glucose levels did not decrease proportionally to the insulin increase. We hypothesized that BPA and OP disrupt calcium homeostasis resulting in insulin resistance through induction of endoplasmic reticulum (ER) stress. BPA and OP treatment leads to survival of pancreatic β cells against streptozotocin, but despite an increased insulin level, serum glucose regulation is not properly regulated...
June 20, 2018: Toxicology Letters
Bryan T C Chuang, Xiaoguang Liu, Alexander J Lundberg, Tommy J K Toung, John A Ulatowski, Raymond C Koehler
BACKGROUND: Injection of a clot into the internal carotid artery is an experimental model of ischemic stroke that is considered to closely mimic embolic stroke in humans. In this model, the common carotid artery typically remains temporarily occluded to permit time for stabilization of the clot in the middle cerebral artery. However, the associated lengthening of the anesthesia duration could affect arterial blood pressure and stroke outcome. NEW METHOD: We refined the model by examining how increasing isoflurane anesthesia duration from 30 to 60 min after clot embolization affects mortality, infarct volume, edema, blood-brain barrier permeability, and the 8-h post-ischemic time course of blood pressure, which has not been reported previously in this model...
June 20, 2018: Journal of Neuroscience Methods
Florence Guignot, Dominique Fortini, Stéphane Grateau, Cécile Burette, Virginie Dreyfus, Carole Moreau-Vauzelle, Pierrick Aupinel, Elisabeth Blesbois
Honeybees, major providers of pollination, are endangered in many areas. Embryo cryopreservation may be a very useful tool to maintain their genetic diversity. However, it is complex in insects, because embryos are chill sensitive and are surrounded by two protectant membranes, the chorion and vitelline. These membranes prevent penetration of cryoprotectant in the embryos. This study aimed to test different conditions of embryo preparation before cryopreservation, including low-frequency sonophoresis, a physical method of permeabilization, and passages through cryoprotectant solutions...
June 20, 2018: Cryobiology
Siok-Fong Chin, Junaida Osman, Rahman Jamal
A simple and economical method has been developed for simultaneous determination of human serum 25-hydroxyvitamin D2 (25OHD2 ) and 25-hydroxyvitamin D3 (25OHD3 ) using Ultra Performance Liquid Chromatography (UPLC). Non-human matrix of 4% BSA was used to construct the calibration curve and in quality control samples' preparation to avoid interference of the endogenous 25-hydroxyvitamin D (25OHD) present in the human serum. 25OHD2 , 25OHD3 and dodecanophenone (internal standard, IS) were separated on a CORTECS solid-core particle column and monitored by photodiode array detector at wavelength of 265 nm within five min run time...
June 20, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
Maycon I O Milanez, Érika E Nishi, Alex Y S Sato, Henrique A Futuro Neto, Cássia T Bergamaschi, Ruy R Campos
The role of spinal cord neurons in renal sympathoexcitation remains unclear in renovascular hypertension, represented by the 2-kidney, 1-clip (2K1C) model. Thus, we aimed to assess the influence of spinal glutamatergic and AT1 angiotensin II receptors on renal sympathetic nerve activity (rSNA) in 2K1C Wistar rats. Hypertension was induced by clipping the renal artery with a silver clip. After six weeks, a catheter (PE-10) was inserted into the subarachnoid space and advanced to the T10-11 vertebral level in urethane-anaesthetized rats...
June 20, 2018: Brain Research
Giuseppe Lippi, Gian Luca Salvagno, Simona Lampus, Elisa Danese, Matteo Gelati, Chiara Bovo, Martina Montagnana, Ana-Maria Simundic
BACKGROUND: Although it is known that glucose concentration exhibits a time-dependent decay in uncentrifuged serum and lithium-heparin blood tubes, no evidence exists on how this variation may depend on blood cell counts (CBC) and volumes. METHODS: Venous blood was drawn from 30 non fasting healthy volunteers into three serum and three lithium-heparin tubes. One serum and lithium-heparin tubes were centrifuged within 15 min after collection and glucose was measured with a hexokinase assay...
June 23, 2018: Clinical Chemistry and Laboratory Medicine: CCLM
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