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https://www.readbyqxmd.com/read/29141533/targeting-cancer-stem-cells-and-non-stem-cancer-cells-the-potential-of-lipid-based-nanoparticles
#1
Ana Filipa Cruz, Nuno Andre Fonseca, Vera Moura, Sergio Simoes, Joao Nuno Moreira
Background Cancer stem cells (CSCs) have been described as a relevant contributor to tumorigenicity, metastasis, tumor recurrence and drug resistance, making this cell population a relevant target in solid tumors. METHODS: This has stimulated the development of different therapeutic strategies often targeting surface markers (CD44, epithelial cell adhesion molecule (EpCAM), aldehyde dehydrogenase (ALDH) and nucleolin) and/or signaling pathways that are aberrantly activated and contribute to CSCs proliferation and survival...
November 14, 2017: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/29123088/a-water-soluble-nucleolin-aptamer-paclitaxel-conjugate-for-tumor-specific-targeting-in-ovarian-cancer
#2
Fangfei Li, Jun Lu, Jin Liu, Chao Liang, Maolin Wang, Luyao Wang, Defang Li, Houzong Yao, Qiulong Zhang, Jia Wen, Zong-Kang Zhang, Jie Li, Quanxia Lv, Xiaojuan He, Baosheng Guo, Daogang Guan, Yuanyuan Yu, Lei Dang, Xiaohao Wu, Yongshu Li, Guofen Chen, Feng Jiang, Shiguo Sun, Bao-Ting Zhang, Aiping Lu, Ge Zhang
Paclitaxel (PTX) is among the most commonly used first-line drugs for cancer chemotherapy. However, its poor water solubility and indiscriminate distribution in normal tissues remain clinical challenges. Here we design and synthesize a highly water-soluble nucleolin aptamer-paclitaxel conjugate (NucA-PTX) that selectively delivers PTX to the tumor site. By connecting a tumor-targeting nucleolin aptamer (NucA) to the active hydroxyl group at 2' position of PTX via a cathepsin B sensitive dipeptide bond, NucA-PTX remains stable and inactive in the circulation...
November 9, 2017: Nature Communications
https://www.readbyqxmd.com/read/29111347/n6l-pseudopeptide-interferes-with-nucleophosmin-protein-protein-interactions-and-sensitizes-leukemic-cells-to-chemotherapy
#3
A De Cola, M Franceschini, A Di Matteo, G Colotti, R Celani, E Clemente, R Ippoliti, A M Cimini, A C Dhez, B Vallée, F Raineri, I Cascone, D Destouches, V De Laurenzi, J Courty, L Federici
NPM1 is a multifunctional nucleolar protein implicated in several processes such as ribosome maturation and export, DNA damage response and apoptotic response to stress stimuli. The NPM1 gene is involved in human tumorigenesis and is found mutated in one third of acute myeloid leukemia patients, leading to the aberrant cytoplasmic localization of NPM1. Recent studies indicated that the N6L multivalent pseudopeptide, a synthetic ligand of cell-surface nucleolin, is also able to bind NPM1 with high affinity. N6L inhibits cell growth with different mechanisms and represents a good candidate as a novel anticancer drug for a number of malignancies of different histological origin...
October 27, 2017: Cancer Letters
https://www.readbyqxmd.com/read/29108569/a-genome-inspired-reverse-selection-approach-to-aptamer-discovery
#4
Christina M Albanese, Suttipong Suttapitugsakul, Shruthi Perati, Linda B McGown
Limitations of Systematic Evolution of Ligands by Exponential Enrichment (SELEX) and related methods that depend upon combinatorial oligonucleotide libraries have hindered progress in this area. Our laboratory has introduced a new approach to aptamer discovery that uses oligonucleotides with sequences drawn from the human genome to capture proteins from biological samples. Specifically, we have focused on capture of proteins in nuclear extracts from human cell lines using G-quadruplex (G4) forming genomic sequences...
January 15, 2018: Talanta
https://www.readbyqxmd.com/read/29097213/mitochondrial-catalase-induces-cells-transformation-through-nucleolin-dependent-cox-2-mrna-stabilization
#5
Xin Liao, Chao Huang, Dongyun Zhang, Jingjing Wang, Jingxia Li, Honglei Jin, Chuanshu Huang
It's well documented that over-production of reactive oxygen species (ROS) causes detrimental damages to cells. While a low level of ROS, such as H2O2, functions as signaling transducer and motivates cell proliferation in both cancer and non-transformed stem cells. As a double-edged sword, the direct evidence for demonstrating the function of H2O2 in the cause of tumor is barely characterized in intact cells. In our current study, we found that targeted expression of mitochondrial catalase (mCAT), but not catalase, could significantly reduce the accumulation of H2O2 in mouse epithelial JB6 Cl41 cells, consequently led to the cell malignant transformation and anchorage-independent cell growth...
October 31, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/29071183/satellite-like-gold-nanocomposites-for-targeted-mass-spectrometry-imaging-of-tumor-tissues
#6
Yu-Ting Tseng, Scott G Harroun, Chien-Wei Wu, Ju-Yi Mao, Huan-Tsung Chang, Chih-Ching Huang
We have developed a simple, rapid, high-throughput cancer diagnosis system using functional nanoparticles (NPs) consisting of poly(catechin) capped-gold NPs (Au@PC NPs) and smaller nucleolin-binding aptamer (AS1411) conjugated gold NPs (AS1411-Au NPs). The AS1411-Au NPs/Au@PC NP is used as a targeting agent in laser desorption/ionization mass spectrometry (LDI-MS)-based tumor tissue imaging. Self-assembled core-shell Au@PC NPs are synthesized by a simple reaction of tetrachloroaurate(III) with catechin. Au@PC NPs with a well-defined and dense poly(catechin) shell (~40-60 nm) on the surface of each Au core (~60-80 nm) are obtained through careful control of the ratio of catechin to gold ions, as well as the pH of the reaction solution...
2017: Nanotheranostics
https://www.readbyqxmd.com/read/29031805/aptamer-targeted-delivery-of-bcl-xl-shrna-using-alkyl-modified-pamam-dendrimers-into-lung-cancer-cells
#7
Sara Ayatollahi, Zahra Salmasi, Maryam Hashemi, Saeedeh Askarian, Reza Kazemi Oskuee, Khalil Abnous, Mohammad Ramezani
RNAi-based gene therapy has been recently considered as a promising approach against cancer. Targeted delivery of drug, gene or therapeutic RNAi-based systems to tumor cells is one of the important issues in order to reduce side effects on normal cells. Several strategies have been developed to improve the safety and selectivity of cancer treatments including antibodies, peptides and recently aptamers with various attractive characteristics including higher target specificity, affinity and reduced toxicity...
October 12, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28993479/lamin-b2-modulates-nucleolar-morphology-dynamics-and-function
#8
Ayantika Sen Gupta, Kundan Sengupta
The nucleolus is required for ribosome biogenesis. Human cells have 2-3 nucleoli associated with Nucleolar Organizer Region (NOR) bearing chromosomes. Increase in number and altered nucleolar morphology define cancer cells. However, the mechanisms that modulate nucleolar morphology and function are unclear. Here we show that in addition to the nuclear envelope, Lamin B2 localizes proximal to Nucleolin at the Granular Component (GC) of the nucleolus and associates with nucleolar proteins - Nucleolin and Nucleophosmin...
October 9, 2017: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/28991436/aptamer-modified-tetrahedral-dna-nanostructure-for-tumor-targeted-drug-delivery
#9
Qianshun Li, Dan Zhao, Xiaoru Shao, Shiyu Lin, Xueping Xie, Mengting Liu, Wenjuan Ma, Sirong Shi, Yunfeng Lin
Tetrahedral DNA nanostructures (TDNs) are considered promising drug delivery carriers because they are able to permeate cellular membrane and are biocompatible and biodegradable. Furthermore, they can be modified by functional groups. To improve the drug-delivering ability of TDNs, we chose anticancer aptamer AS1411 to modify TDNs for tumor-targeted drug delivery. AS1411 can specifically bind to nucleolin, which is overexpressed on the cell membrane of tumor cells. Furthermore, AS1411 can inhibit NF-κB signaling and reduce the expression of bcl-2...
October 16, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28989617/stimuli-responsive-nucleic-acid-functionalized-metal-organic-framework-nanoparticles-using-ph-and-metal-ion-dependent-dnazymes-as-locks
#10
Wei-Hai Chen, Xu Yu, Alessandro Cecconello, Yang Sung Sohn, Rachel Nechushtai, Itamar Willner
A versatile approach to modify metal-organic framework nanoparticles (NMOFs) with nucleic acid tethers, using the "click chemistry" method is introduced. The nucleic acid-functionalized NMOFs are used to prepare stimuli-responsive carriers of loads (fluorescence probes or anti-cancer drugs). Two different stimuli-responsive nucleic acid-based NMOFs are presented. One system involves the preparation of pH-responsive NMOFs. The NMOFs are loaded with fluorophores or doxorubicin anti-cancer drug and locked in the NMOFs by pH-responsive DNA duplex capping units...
August 1, 2017: Chemical Science
https://www.readbyqxmd.com/read/28979207/3-mercaptopyruvate-sulfurtransferase-not-cystathionine-%C3%AE-synthase-nor-cystathionine-%C3%AE-lyase-mediates-hypoxia-induced-migration-of-vascular-endothelial-cells
#11
Beibei Tao, Rui Wang, Chen Sun, Yichun Zhu
Hypoxia-induced angiogenesis is a common phenomenon in many physiological and patho-physiological processes. However, the potential differential roles of three hydrogen sulfide producing systems cystathionine γ-lyase (CSE)/H2S, cystathionine β-synthase (CBS)/H2S, and 3-mercaptopyruvate sulfurtransferase (MPST)/H2S in hypoxia-induced angiogenesis are still unknown. We found that minor hypoxia (10% oxygen) significantly increased the migration of vascular endothelial cells while hypoxia (8% oxygen) significantly inhibited cell migration...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28941284/targeted-therapy-of-human-glioblastoma-via-delivery-of-a-toxin-through-a-peptide-directed-to-cell-surface-nucleolin
#12
Dhez Anne-Chloé, Benedetti Elisabetta, Antonosante Andrea, Panella Gloria, Ranieri Brigida, Florio Tiziana Marilena, Cristiano Loredana, Angelucci Francesco, Giansanti Francesco, Di Leandro Luana, d'Angelo Michele, Melone Marina, De Cola Antonella, Federici Luca, Galzio Renato, Cascone Ilaria, Raineri Fabio, Cimini Annamaria, Courty José, Giordano Antonio, Ippoliti Rodolfo
Targeted anticancer therapies demand discovery of new cellular targets to be exploited for the delivery of toxic molecules and drugs. In this perspective, in the last few years, nucleolin has been identified as an interesting surface marker to be used for the therapy of glioblastoma. In this study, we investigated whether a synthetic antagonist of cell-surface nucleolin known as N6L, previously reported to decrease both tumour growth and tumour angiogenesis in several cancer cell lines, including glioblastoma cells, as well as endothelial cells proliferation, could be exploited to deliver a protein toxin (saporin) to glioblastoma cells...
September 23, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28928437/as1411-aptamer-anionic-linear-globular-dendrimer-g2-iohexol-selective-nano-theranostics
#13
Pardis Mohammadzadeh, Reza Ahangari Cohan, Seyedeh Masoumeh Ghoreishi, Ahmad Bitarafan-Rajabi, Mehdi Shafiee Ardestani
Molecular theranostics is of the utmost interest for diagnosis as well as treatment of different malignancies. In the present study, anionic linear globular dendrimer G2 is employed as a suitable carrier for delivery and AS1411 aptamer is exploited as the targeting agent to carry Iohexol specifically to the human breast cancer cells (MCF-7). Dendrimer G2 was prepared and conjugation of dendrimer and aptamer was carried out thereafter. Based on the data yielded by AFM, morphology of smooth and spherical non-targeted dendrimer changed to the rough aspherical shape when it conjugated...
September 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28925950/targeting-host-cell-surface-nucleolin-for-rsv-therapy-challenges-and-opportunities
#14
Peter Mastrangelo, Michael J Norris, Wenming Duan, Edward G Barrett, Theo J Moraes, Richard G Hegele
Nucleolin (NCL) has been reported as a cellular receptor for the human respiratory syncytial virus (RSV). We studied the effects of re-purposing AS1411, an anti-cancer compound that binds cell surface NCL, as a possible novel strategy for RSV therapy in vitro and in vivo. AS1411 was administered to RSV-infected cultures of non-polarized (HEp-2) and polarized (MDCK) epithelial cells and to virus-infected mice and cotton rats. Results of in vitro experiments showed that AS1411, used in micromolar concentrations, was associated with decreases in the number of virus-positive cells...
September 19, 2017: Vaccines
https://www.readbyqxmd.com/read/28913345/a-yeast-model-for-the-mechanism-of-the-epstein-barr-virus-immune-evasion-identifies-a-new-therapeutic-target-to-interfere-with-the-virus-stealthiness
#15
COMMENT
María José Lista, Rodrigo Prado Martins, Gaelle Angrand, Alicia Quillévéré, Chrysoula Daskalogianni, Cécile Voisset, Marie-Paule Teulade-Fichou, Robin Fåhraeus, Marc Blondel
The oncogenic Epstein-Barr virus (EBV) evades the immune system but has an Achilles heel: its genome maintenance protein EBNA1. Indeed, EBNA1 is essential for viral genome replication and maintenance but also highly antigenic. Hence, EBV evolved a system in which the glycine-alanine repeat (GAr) of EBNA1 limits the translation of its own mRNA at a minimal level to ensure its essential function thereby, at the same time, minimizing immune recognition. Defining intervention points where to interfere with EBNA1 immune evasion is an important step to trigger an immune response against EBV-carrying cancers...
August 31, 2017: Microbial Cell
https://www.readbyqxmd.com/read/28900213/copine-7-binds-to-the-cell-surface-receptor-nucleolin-and-regulates-ciliogenesis-and-dspp-expression-during-odontoblast-differentiation
#16
You-Mi Seo, Su-Jin Park, Hye-Kyung Lee, Joo-Cheol Park
Tooth development is a progressive process regulated by interactions between epithelial and mesenchymal tissues. Our previous studies showed that copine-7 (Cpne7), a dental epithelium-derived protein, is a signalling molecule that is secreted by preameloblasts and regulates the differentiation of preodontoblasts into odontoblasts. However, the mechanisms involved in the translocation of Cpne7 from preameloblasts to preodontoblasts and the functions of Cpne7 during odontogenesis are poorly understood. Here, we showed that the internalization of Cpne7 was mediated primarily by caveolae...
September 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28874822/carbon-dots-as1411-aptamer-nanoconjugate-for-ultrasensitive-spectrofluorometric-detection-of-cancer-cells
#17
Hasan Motaghi, Masoud Ayatollahi Mehrgardi, Philippe Bouvet
In the present study, a sensitive and selective signal-on method for aptamer based spectrofluorometric detection of cancer cells is introduced. AS1411, a nucleolin aptamer, is wrapped around water-soluble carbon dots and used as a probe for the detection of several types of cancer cells. Nucleolin, is overexpressed on the surface of cancer cells. Mouse breast 4T1, human breast MCF7, and human cervical HeLa cancer cells were selected as target cells, while human foreskin fibroblast cells HFFF-PI6 served as control cells...
September 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28868110/erratum-quick-chip-assay-using-locked-nucleic-acid-modified-epithelial-cell-adhesion-molecule-and-nucleolin-aptamers-for-the-capture-of-circulating-tumor-cells-biomicrofluidics-9-5-054110-2015
#18
Nihal G Maremanda, Kislay Roy, Rupinder K Kanwar, Vidyarani Shyamsundar, Vijayalakshmi Ramshankar, Arvind Krishnamurthy, Subramanian Krishnakumar, Jagat R Kanwar
[This corrects the article DOI: 10.1063/1.4930983.].
July 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28841406/g-quadruplex-and-calf-thymus-dna-interaction-of-quaternized-tetra-and-octa-pyridyloxy-substituted-indium-iii-phthalocyanines
#19
Efkan Bağda, Esra Bağda, Mahmut Durmuş
The interactions of small molecules with G-quadruplex and double stranded DNA are important due to their potential biological and medical usages. In the present paper, the interactions of indium (III) phthalocyanines (quaternized 2,3,9,10,16,17,23,24-octakis-[(3-pyridyloxy) phthalocyaninato] chloroindium(III): OInPc and quaternized 2(3),9(10),16(17),23(24)-tetrakis-[(3-pyridyloxy) phthalocyaninato] chloroindium(III): TInPc) with hybrid G-quadruplex (Tel 21) and parallel G-quadruplexes (nucleolin, KRAS, c-MYC, vegf) were studied...
August 7, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28827664/integrated-analysis-of-mrna-and-mirna-expression-in-hela-cells-expressing-low-levels-of-nucleolin
#20
Sanjeev Kumar, Elizabhet Cruz Gomez, Mounira Chalabi-Dchar, Cong Rong, Sadhan Das, Iva Ugrinova, Xavier Gaume, Karine Monier, Fabien Mongelard, Philippe Bouvet
Nucleolin is an essential protein that plays important roles in the regulation of cell cycle and cell proliferation. Its expression is up regulated in many cancer cells but its molecular functions are not well characterized. Nucleolin is present in the nucleus where it regulates gene expression at the transcriptional and post-transcriptional levels. Using HeLa cells depleted in nucleolin we performed an mRNA and miRNA transcriptomics analysis to identify biological pathways involving nucleolin. Bioinformatic analysis strongly points to a role of nucleolin in lipid metabolism, and in many signaling pathways...
August 21, 2017: Scientific Reports
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