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https://www.readbyqxmd.com/read/28319022/recovery-of-lignin-peroxidase-from-submerged-liquid-fermentation-of-amauroderma-rugosum-blume-t-nees-torrend-using-polyethylene-glycol-salt-aqueous-two-phase-system
#1
Wan Yng Linda Jong, Pau Loke Show, Tau Chuan Ling, Yee Shin Tan
Amauroderma rugosum is a wild mushroom species widely distributed in tropics and is classified under the class of Basidiomycetes. Basidiomycetes are well-known for their abilities of producing lignocellulolytic enzymes such as lignin peroxidase (LiP), laccase (Lac) and manganese peroxidase (MnP). Different factors such as nutrient sources, incubation period and agitation affect the production of lignocellulolytic enzymes. The A. rugosum produced LiP in the medium supplemented with potato dextrose broth (PDB), 0...
March 16, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28317109/what-if-plant-functional-types-conceal-species-specific-responses-to-environment-study-on-arctic-shrub-communities
#2
Patrick Saccone, Kristiina Hoikka, Risto Virtanen
Plant functional types (PFT) are increasingly used to outline biome-scale plant-environment relationship and predict global change effects on community structure. However, the potentials and limitations of the PFT approach have to be tested as they can be less sensitive than trait-based or species-level approaches. Here, we compare the responses of deciduous-evergreen shrub PFTs and species to gradual snow-related environmental conditions by also considering effects of aboveground architectural traits and neighboring shrubs...
March 19, 2017: Ecology
https://www.readbyqxmd.com/read/28301535/quantitative-assessment-of-pulmonary-alveolar-proteinosis-pap-with-ultra-dose-ct-and-correlation-with-pulmonary-function-tests-pfts
#3
Xin Sui, Qianni Du, Kai-Feng Xu, Xinlun Tian, Lan Song, Xiao Wang, Xiaoli Xu, Zixing Wang, Yuyan Wang, Jun Gu, Wei Song, Zhengyu Jin
BACKGROUND: The purpose of this study was to investigate whether ultra-low-dose chest computed tomography (CT) can be used for visual assessment of CT features in patients with pulmonary alveolar proteinosis (PAP) and to evaluate the relationship between the quantitative analysis of the ultra-low-dose CT scans and the pulmonary function tests (PFTs). METHODS: Thirty-eight patients (mean [SD] age, 44.47 [12.28] years; 29 males, 9 females) with PAP were enrolled and subjected to two scans each with low-dose CT (reference parameters: 120 kV and 50 mAs) and ultra-low-dose CT (reference parameters, 80 kV, 25 mAs)...
2017: PloS One
https://www.readbyqxmd.com/read/28294159/to-find-a-better-dosimetric-parameter-in-the-predicting-of-radiation-induced-lung-toxicity-individually-ventilation-perfusion-or-ct-based
#4
Lin-Lin Xiao, Guoren Yang, Jinhu Chen, Xiaohui Wang, Qingwei Wu, Zongwei Huo, Qingxi Yu, Jinming Yu, Shuanghu Yuan
This study aimed to find a better dosimetric parameter in predicting of radiation-induced lung toxicity (RILT) in patients with non-small cell lung cancer (NSCLC) individually: ventilation(V), perfusion (Q) or computerized tomography (CT) based. V/Q single-photon emission computerized tomography (SPECT) was performed within 1 week prior to radiotherapy (RT). All V/Q imaging data was integrated into RT planning system, generating functional parameters based on V/Q SPECT. Fifty-seven NSCLC patients were enrolled in this prospective study...
March 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28292729/pre-analytical-effects-of-pneumatic-tube-system-transport-on-routine-haematology-and-coagulation-tests-global-coagulation-assays-and-platelet-function-assays
#5
Sandra Le Quellec, Mickaël Paris, Christophe Nougier, Frédéric Sobas, Lucia Rugeri, Sandrine Girard, Jean-Claude Bordet, Claude Négrier, Yesim Dargaud
INTRODUCTION: Pneumatic tube system (PTS) in hospitals is commonly used for the transport of blood samples to clinical laboratories, as it is rapid and cost-effective. The aim was to compare the effects on haematology samples of a newly acquired ~2km-long PTS that links 2 hospitals with usual transport (non-pneumatic tube system, NPTS). METHODS: Complete blood cell count, routine coagulation assays, platelet function tests (PFT) with light-transmission aggregometry and global coagulation assays including ROTEM® and thrombin generation assay (TGA) were performed on blood samples from 30 healthy volunteers and 9 healthy volunteers who agreed to take aspirin prior to blood sampling...
December 28, 2016: Thrombosis Research
https://www.readbyqxmd.com/read/28285946/development-of-rostral-inferior-parietal-lobule-area-functional-connectivity-from-late-childhood-to-early-adulthood
#6
Mengxing Wang, Jilei Zhang, Guangheng Dong, Hui Zhang, Haifeng Lu, Xiaoxia Du
Although the mirror neuron system (MNS) has been extensively studied in monkeys and adult humans, very little is known about its development. Previous studies suggest that the MNS is present by infancy and that the brain and MNS-related cognitive abilities (such as language, empathy, and imitation learning) continue to develop after childhood. In humans, the PFt area of the inferior parietal lobule (IPL) seems to particularly correlate with the functional properties of the PF area in primates, which contains mirror neurons...
March 7, 2017: International Journal of Developmental Neuroscience
https://www.readbyqxmd.com/read/28281736/optimal-antiplatelet-pharmacotherapy-guided-by-bedside-genetic-or-functional-testing-in-elective-pci-patients-a-pilot-study-onside-test-pilot
#7
Lukasz Koltowski, Mariusz Tomaniak, Daniel Aradi, Zenon Huczek, Krzysztof J Filipiak, Janusz Kochman, Sylwia Gajda, Paweł Balsam, Grzegorz Opolski
BACKGROUND: Dual antiplatelet therapy is recommended after elective percutaneous coronary intervention (PCI) in stable coronary artery disease (SCAD) patients; however, still one-third of patients do not obtain adequate platelet inhibition that may result in increased cardiovascular risk. The aim of the ONSIDE TEST study is to evaluate the clinical impact of point-of-care genotyping- and platelet function-based personalized dual antiplatelet strategies in SCAD individuals undergoing PCI...
March 10, 2017: Cardiology Journal
https://www.readbyqxmd.com/read/28279683/long-term-pulmonary-complications-in-perinatally-hiv-infected-youth
#8
William T Shearer, Denise L Jacobson, Wendy Yu, George K Siberry, Murli Purswani, Suzanne Siminski, Laurie Butler, Erin Leister, Gwendolyn Scott, Russell B Van Dyke, Ram Yogev, Mary E Paul, Ana Puga, Andrew A Colin, Meyer Kattan
BACKGROUND: Increased incidence and prevalence of asthma have been documented for perinatally HIV-infected (HIV) youth 10-21 years of age (youth) compared to HIV-exposed uninfected (HEU) youth. OBJECTIVE: To perform objective pulmonary function tests (PFTs) in HIV and HEU youth with and without diagnosed asthma. METHOD: Asthma was determined in 370 participants (218 HIV, 152 HEU) by chart review and self-report at 13 sites. Interpretable PFTs (188 HIV, 132 HEU) were classified as obstructive, restrictive, or normal and reversibility was determined after bronchodilator inhalation...
March 6, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28277322/utilizing-forced-vital-capacity-to-predict-low-lung-compliance-and-select-intraoperative-tidal-volume-during-thoracic-surgery
#9
Nir Hoftman, Eric Eikermann, John Shin, Jack Buckley, Kaveh Navab, Fereidoun Abtin, Tristan Grogan, Maxime Cannesson, Aman Mahajan
BACKGROUND: Tidal volume selection during mechanical ventilation utilizes dogmatic formulas that only consider a patient's predicted body weight (PBW). In this study, we investigate whether forced vital capacity (FVC) (1) correlates better to total lung capacity (TLC) than PBW, (2) predicts low pulmonary compliance, and (3) provides an alternative method for tidal volume selection. METHODS: One hundred thirty thoracic surgery patients had their preoperative TLC calculated via 2 methods: (1) pulmonary function test (PFT; TLCPFT) and (2) computed tomography 3D reconstruction (TLCCT)...
March 8, 2017: Anesthesia and Analgesia
https://www.readbyqxmd.com/read/28277058/the-interaction-between-preoperative-platelet-count-and-function-and-its-relationship-with-postoperative-bleeding-in-cardiac-surgery
#10
Marco Ranucci, Ekaterina Baryshnikova
Platelet function tests (PFTs) before cardiac surgery are predictive of postoperative bleeding and can guide a correct timing of surgery in patients under P2Y12 inhibitors. Thrombocytopenia affects PFT and may determine postoperative bleeding. The present study aims to investigate the relationship between platelet count and function, and its role in determining postoperative bleeding in cardiac surgery patients pre-treated with P2Y12 inhibitors. The study includes 589 consecutive cardiac surgery patients, tested before surgery with platelet count and multiple electrode aggregometry (MEA) ADPtest (investigating P2Y12 receptor platelet reactivity) and TRAPtest (investigating the thrombin-dependent platelet reactivity)...
March 2, 2017: Platelets
https://www.readbyqxmd.com/read/28264721/densitometric-and-local-histogram-based-analysis-of-computed-tomography-images-in-patients-with-idiopathic-pulmonary-fibrosis
#11
Samuel Y Ash, Rola Harmouche, Diego Lassala Lopez Vallejo, Julian A Villalba, Kris Ostridge, River Gunville, Carolyn E Come, Jorge Onieva Onieva, James C Ross, Gary M Hunninghake, Souheil Y El-Chemaly, Tracy J Doyle, Pietro Nardelli, Gonzalo V Sanchez-Ferrero, Hilary J Goldberg, Ivan O Rosas, Raul San Jose Estepar, George R Washko
BACKGROUND: Prior studies of clinical prognostication in idiopathic pulmonary fibrosis (IPF) using computed tomography (CT) have often used subjective analyses or have evaluated quantitative measures in isolation. This study examined associations between both densitometric and local histogram based quantitative CT measurements with pulmonary function test (PFT) parameters and mortality. In addition, this study sought to compare risk prediction scores that incorporate quantitative CT measures with previously described systems...
March 7, 2017: Respiratory Research
https://www.readbyqxmd.com/read/28258938/ventilation-distribution-and-small-airway-function-in-patients-with-systemic-sclerosis
#12
B R A Silva, R Rufino, C H Costa, V S Vilela, R A Levy, A J Lopes
BACKGROUND: Despite the importance of traditional pulmonary function tests (PFTs) in managing systemic sclerosis (SSc), many patients with pulmonary disease diagnosed by computed tomography (CT) present with normal PFTs. OBJECTIVE: To evaluate the efficacy of the nitrogen single-breath washout (N2SBW) test in diagnosing SSc and to correlate N2SBW parameters with the PFT indexes used in the follow-up of these patients, clinical data, and CT findings. METHODS: Cross-sectional study in which 52 consecutive SSc patients were subjected to spirometry, body plethysmography, analysis of the diffusing capacity for carbon monoxide (DLCO), analysis of respiratory muscle strength, N2SBW testing, and CT analysis...
March 1, 2017: Revista Portuguesa de Pneumologia
https://www.readbyqxmd.com/read/28245756/diosmetin-induces-cell-apoptosis-by-regulating-cyp1a1-cyp1a2-due-to-p53-activation-in-hepg2-cells
#13
Bin Liu, Kaiqiao Jia, Yu Yang, Siyuan Hao, Caijie Lu, Fengming Xu, Dehui Feng, Runzhi Zhu
It was explored that CYP1 family of cytochromes P450 were over-expressed in several types of cancer. Our study aimed to characterize anti-proliferative activity and metabolism of the natural flavonoid diosmetin in the human hepatoma cell HepG2, expressing CYP1 family. Dios-induced cell apoptosis could be reversed due to p53 blockade and the cellular P53 and CYP1A1/CYP1A2 proteins levels were examined. P53 and CYP1A1/CYP1A2 proteins were up-regulated by Dios; when PFT-α was added into cells, the P53 levels were down-regulated accompanied with up-regulated CYP1A1/CYP1A2...
February 27, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28243079/can-we-predict-the-prognosis-of-copd-with-a-routine-blood-test
#14
Wei Xiong, Mei Xu, Yunfeng Zhao, Xueling Wu, Bigyan Pudasaini, Jin-Ming Liu
BACKGROUND: The major characteristic of COPD is systemic inflammation. The parameters such as neutrophil-to-lymphocyte ratio (NLR) and eosinophil-to-basophil ratio (EBR) in routine blood test (RBT) are considered to be the underlying biomarkers of inflammation. We hypothesized that the prognosis of patients with COPD can be predicted with RBT. METHODS: Patients with COPD in stable stage were enrolled. The RBT, pulmonary function testing (PFT), BODE index, C-reactive protein (CRP), procalcitonin, and erythrocyte sedimentation rate (ESR) were performed at enrollment and every follow-up once in every 3 months during the 24-month follow-up period...
2017: International Journal of Chronic Obstructive Pulmonary Disease
https://www.readbyqxmd.com/read/28241532/understanding-the-contribution-of-phytoplankton-phase-functions-to-uncertainties-in-the-water-colour-signal
#15
Lisl Robertson Lain, Stewart Bernard, Mark W Matthews
The accurate description of a water body's volume scattering function (VSF), and hence its phase functions, is critical to the determination of the constituent inherent optical properties (IOPs), the associated spectral water-leaving reflectance, and consequently the retrieval of phytoplankton functional type (PFT) information. The equivalent algal populations (EAP) model has previously been evaluated for phytoplankton-dominated waters, and offers the ability to provide phytoplankton population-specific phase functions, unveiling a new opportunity to further understanding of the causality of the PFT signal...
February 20, 2017: Optics Express
https://www.readbyqxmd.com/read/28240165/diosmetin-a-potential-p53-activator-performs-anticancer-effect-by-regulating-cell-cycling-and-cell-proliferation-in-hepg2-cells
#16
Bin Liu, Miaomiao Zhang, Shuna Liu, Ying Jie, Jingjing Zhang, Weiqiang Zheng, Kurihara Hiroshi, Runzhi Zhu
Diosmetin (Dios), a flavone aglycone, is a major active ingredient of many herbal medicines. Previous studies implicated that the potential antitumor activities of Dios are mediated through multiple cell signaling pathways, however more investigations are required to reveal the targets and mechanisms of Dios. In this study, our results demonstrated that cell cycle progression and proliferation of HepG2 cells were inhibited by Dios. Meanwhile, Dios-induced liver cancer cell apoptosis was related with the p53 activation...
February 22, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28237798/p53-mediated-oligodendrocyte-apoptosis-initiates-demyelination-after-compressed-spinal-cord-injury-by-enhancing-er-mitochondria-interaction-and-e2f1-expression
#17
Lan Ma, Hai-Jun Yu, Sheng-Wei Gan, Rui Gong, Ke-Jie Mou, Jun Xue, Shan-Quan Sun
Oligodendrocyte apoptosis mediated demyelination is a pathological change characteristic of compressed spinal cord injury (CSCI). However, the mechanism of demyelination due to oligodendrocyte apoptosis is not known. In this study, after successfully establishing a rat CSCI model using a custom-made compressor, we investigated the pathological changes, MBP expression, as well as apoptosis-related protein (p53, active caspase-3) expression to determine whether or not apoptosis and demyelination occurred after injury...
February 24, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28218486/determinants-and-outcomes-of-upfront-surgery-versus-medical-therapy-for-chronic-rhinosinusitis-in-cystic-fibrosis
#18
Noel Ayoub, Andrew Thamboo, Al-Rahim Habib, Jayakar V Nayak, Peter H Hwang
BACKGROUND: The indications for surgical management of chronic rhinosinusitis (CRS) in patients with cystic fibrosis (CF) are poorly defined. In this study we compare outcomes of medical versus surgical treatment and examine trends associated with the transition from medical to surgical therapy in CF patients. METHODS: One hundred thirty-six patients with CF referred to a tertiary rhinology practice were retrospectively divided into 3 cohorts: Medical, Upfront Surgery, or Crossover, if they converted from medical to surgical management...
February 20, 2017: International Forum of Allergy & Rhinology
https://www.readbyqxmd.com/read/28217888/computed-tomography-airway-lumen-volumetry-in-patients-with-acromegaly-association-with-growth-hormone-levels-and-lung-function
#19
Gustavo Bittencourt Camilo, Alysson Roncally Silva Carvalho, Alan Ranieri Medeiros Guimarães, Leandro Kasuki, Mônica Roberto Gadelha, Roberto Mogami, Pedro Lopes de Melo, Agnaldo José Lopes
INTRODUCTION: The segmentation and skeletonisation of images via computed tomography (CT) airway lumen volumetry provide a new perspective regarding the incorporation of this technique in medical practice. Our aim was to quantify morphological changes in the large airways of patients with acromegaly through CT and, secondarily, to correlate these findings with hormone levels and pulmonary function testing (PFT) parameters. METHODS: This was a cross-sectional study in which 28 non-smoker patients with acromegaly and 15 control subjects underwent CT analysis of airway lumen volumetry with subsequent image segmentation and skeletonisation...
February 19, 2017: Journal of Medical Imaging and Radiation Oncology
https://www.readbyqxmd.com/read/28208871/clinical-utility-of-fractional-exhaled-nitric-oxide-feno-as-a-biomarker-to-predict-severity-of-disease-and-response-to-inhaled-corticosteroid-ics-in-asthma-patients
#20
Revathy Neelamegan, Vinodkumar Saka, Kadhiravan Tamilarasu, Manju Rajaram, Sandhiya Selvarajan, Adithan Chandrasekaran
INTRODUCTION: Bronchial asthma is a common chronic inflammatory airway disease diagnosed and is based on symptomatic history and Pulmonary Function Tests (PFT). Fractional exhaled Nitric Oxide (FeNO) is exclusively a non-invasive biomarker of on-going eosinophilic airway inflammation which remains unpredictable only with PFTs. FeNO measurement is recommended in predicting asthma severity and Inhaled Corticosteroid (ICS) response but further research is required to understand its clinical utility and agreement with current recommendations in a specific population...
December 2016: Journal of Clinical and Diagnostic Research: JCDR
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