Karan Goswami, Craig Tipton, Samuel Clarkson, Gerard Chang, Timothy L Tan, Brianna Fram, Emanuele Chisari, Keenan Sobol, Roman M Natoli, Janet D Conway, Gregory T Altman, Javad Parvizi, James C Krieg
INTRODUCTION: Fracture nonunion remains a devastating complication and may occur for several reasons, though the microbial contribution remains poorly estimated. Next-generation sequencing (NGS) techniques, including 16S rRNA gene profiling, are capable of rapid bacterial detection within clinical specimens. Nonunion cases may harbor microbes that escape detection by conventional culture methods that contribute to persistence. Our aim was to investigate the application of NGS pathogen detection to nonunion diagnosis...
February 1, 2022: Journal of Orthopaedic Trauma