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https://www.readbyqxmd.com/read/28086806/age-related-macular-degeneration-associated-polymorphism-rs10490924-in-arms2-results-in-deficiency-of-a-complement-activator
#1
Sven Micklisch, Yuchen Lin, Saskia Jacob, Marcus Karlstetter, Katharina Dannhausen, Prasad Dasari, Monika von der Heide, Hans-Martin Dahse, Lisa Schmölz, Felix Grassmann, Medhanie Alene, Sascha Fauser, Harald Neumann, Stefan Lorkowski, Diana Pauly, Bernhard H Weber, Antonia M Joussen, Thomas Langmann, Peter F Zipfel, Christine Skerka
BACKGROUND: Age-related macular degeneration (AMD) is the leading cause of blindness in developed countries. The polymorphism rs10490924 in the ARMS2 gene is highly associated with AMD and linked to an indel mutation (del443ins54), the latter inducing mRNA instability. At present, the function of the ARMS2 protein, the exact cellular sources in the retina and the biological consequences of the rs10490924 polymorphism are unclear. METHODS: Recombinant ARMS2 was expressed in Pichia pastoris, and protein functions were studied regarding cell surface binding and complement activation in human serum using fluoresence-activated cell sorting (FACS) as well as laser scanning microscopy (LSM)...
January 5, 2017: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/28085139/efficacy-of-0-2%C3%A2-%C3%AE-g-day-fluocinolone-acetonide-implant-iluvien-in-eyes-with-diabetic-macular-edema-and-prior-vitrectomy
#2
A Meireles, C Goldsmith, I El-Ghrably, A Erginay, M Habib, B Pessoa, J Coelho, T Patel, R Tadayoni, P Massin, J Atorf, A J Augustin
PurposeLimited data are available on the efficacy of the 0.2 μg/day fluocinolone acetonide (FAc) implant in eyes with prior vitrectomy. Here, we present a collection of 26 vitrectomized eyes treated with the 0.2 μg/day FAc implant.MethodsRetrospective study involving six centers from four European countries analyzing the safety and efficacy data from patients (26 eyes from 25 patients) with DME and a prior vitrectomy that had been treated with one 0.2 μg/day FAc implant.ResultsPrior intravitreal therapies included anti-VEGF (mean, 3...
January 13, 2017: Eye
https://www.readbyqxmd.com/read/28081945/long-term-effects-of-intravitreal-0-19-mg-fluocinolone-acetonide-implant-on-progression-and-regression-of-diabetic-retinopathy
#3
Charles C Wykoff, Usha Chakravarthy, Peter A Campochiaro, Clare Bailey, Ken Green, Jose Cunha-Vaz
OBJECTIVE: To investigate the effects of fluocinolone acetonide (FAc) on the progression to proliferative diabetic retinopathy (PDR) and the impact of FAc on changes in Early Treatment Diabetic Retinopathy Study (ETDRS) diabetic retinopathy (DR) severity scale (DRSS) grade during the Fluocinolone Acetonide in Diabetic Macular Edema (FAME) A and B Phase III clinical trials. DESIGN: Post hoc analysis of data from the 36-month prospective, randomized, FAME A and B trials...
January 9, 2017: Ophthalmology
https://www.readbyqxmd.com/read/28078484/the-effect-of-surface-charges-on-the-cellular-uptake-of-liposomes-investigated-by-live-cell-imaging
#4
Ji Hee Kang, Woo Young Jang, Young Tag Ko
PURPOSE: Liposomes have been developed as versatile nanocarriers for various pharmacological agents. The effect of surface charges on the cellular uptake of the liposomes has been studied by various methods using mainly fixed cells with inevitable limitations. Live cell imaging has been proposed as an alternative methods to overcome the limitations of the fixed cell-based analysis. In this study, we aimed to investigate the effects of surface charges on cellular association and internalization of the liposomes using live cell imaging...
January 11, 2017: Pharmaceutical Research
https://www.readbyqxmd.com/read/28077118/malat1-promoted-invasiveness-of-gastric-adenocarcinoma
#5
Na Keum Lee, Jung Hwa Lee, Cristina Ivan, Hui Ling, Xinna Zhang, Chan Hyuk Park, George A Calin, Sang Kil Lee
BACKGROUND: Gastric cancer is the second leading cause of cancer globally, and the mechanism of its pathogenesis is still largely unknown. Recently, non-coding RNAs have been recognized to promote metastasis in various cancers, including gastric cancer. METHODS: We found that metastasis associated lung adenocarcinoma transcript-1 (MALAT1) is upregulated in gastric cancer tissue compared to adjacent normal tissue, as determined by microarray and subsequent qRT-PCR, then investigated the impact of MALAT1 on apoptosis, cell proliferation, and the cell cycle to dissect the carcinogenesis of gastric cancer, and examined mechanisms of invasion and metastasis...
January 11, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28075510/development-of-a-voided-urine-assay-for-detecting-prostate-cancer-noninvasively-a-pilot-study
#6
Edouard J Trabulsi, Sushil K Tripathi, Leonard Gomella, Charalambos Solomides, Eric Wickstrom, Mathew L Thakur
OBJECTIVE: To validate a hypothesis that prostate cancer (PCa) can be detected noninvasively by a simple and reliable assay by targeting genomic VPAC receptors expressed on malignant PCa cells shed in voided urine. MATERIALS AND METHODS: VPAC receptors were targeted with a specific biomolecule, TP4303, developed in our laboratory. With an IRB "exempt" approval of use of de-identified discarded samples, an aliquot of urine collected as a standard of care, from patients presenting to the urology clinic, (N=207, M= 176, F= 31, 21 years or older) was cytospun...
January 11, 2017: BJU International
https://www.readbyqxmd.com/read/28075486/highly-expandable-human-ips-cell-derived-neural-progenitor-cells-npc-and-neurons-for-central-nervous-system-disease-modeling-and-high-throughput-screening
#7
Chialin Cheng, Daniel M Fass, Kat Folz-Donahue, Marcy E MacDonald, Stephen J Haggarty
Reprogramming of human somatic cells into induced pluripotent stem (iPS) cells has greatly expanded the set of research tools available to investigate the molecular and cellular mechanisms underlying central nervous system (CNS) disorders. Realizing the promise of iPS cell technology for the identification of novel therapeutic targets and for high-throughput drug screening requires implementation of methods for the large-scale production of defined CNS cell types. Here we describe a protocol for generating stable, highly expandable, iPS cell-derived CNS neural progenitor cells (NPC) using multi-dimensional fluorescence activated cell sorting (FACS) to purify NPC defined by cell surface markers...
January 11, 2017: Current Protocols in Human Genetics
https://www.readbyqxmd.com/read/28075349/transthyretin-a-transporter-protein-essential-for-proliferation-of-myoblast-in-the-myogenic-program
#8
Eun Ju Lee, Smritee Pokharel, Arif Tasleem Jan, Soyeon Huh, Richelle Galope, Jeong Ho Lim, Dong-Mok Lee, Sung Wook Choi, Sang-Soep Nahm, Yong-Woon Kim, So-Young Park, Inho Choi
Irregularities in the cellular uptake of thyroid hormones significantly affect muscle development and regeneration. Herein, we report indispensable role of transthyretin (TTR) in maintaining cellular thyroxine level. TTR was found to enhance recruitment of muscle satellite cells to the site of injury, thereby regulating muscle regeneration. Fluorescence-activated cell sorting (FACS) and immunofluorescence analysis of TTRwt (TTR wild type) and TTRkd (TTR knock-down) cells revealed that TTR controlled cell cycle progression by affecting the expression of Cyclin A2...
January 8, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28069704/angiopoietin-1-promotes-atherosclerosis-by-increasing-the-proportion-of-circulating-gr1-monocytes
#9
Takeshi Fujisawa, Keqing Wang, Xi-Lin Niu, Stuart Egginton, Shakil Ahmad, Peter Hewett, Christopher D Kontos, Asif Ahmed
AIMS: Atherosclerosis is a chronic inflammatory disease occurring within the artery wall. A crucial step in atherogenesis is the infiltration and retention of monocytes into the subendothelial space of large arteries induced by chemokines and growth factors. Angiopoietin-1 (Ang-1) regulates angiogenesis and reduces vascular permeability and has also been reported to promote monocyte migration in vitro. We investigated the role of Ang-1 in atherosclerosis-prone apolipoprotein-E (Apo-E) knockout mouse...
January 2017: Cardiovascular Research
https://www.readbyqxmd.com/read/28068321/analysis-of-dna-methylation-in-single-circulating-tumor-cells
#10
C F Pixberg, K Raba, F Müller, B Behrens, E Honisch, D Niederacher, H Neubauer, T Fehm, W Goering, W A Schulz, P Flohr, G Boysen, M Lambros, J S De Bono, W T Knoefel, C Sproll, N H Stoecklein, R P L Neves
Direct analysis of circulating tumor cells (CTCs) can inform on molecular mechanisms underlying systemic spread. Here we investigated promoter methylation of three genes regulating epithelial-to-mesenchymal transition (EMT), a key mechanism enabling epithelial tumor cells to disseminate and metastasize. For this, we developed a single-cell protocol based on agarose-embedded bisulfite treatment, which allows investigating DNA methylation of multiple loci via a multiplex PCR (multiplexed-scAEBS). We established our assay for the simultaneous analysis of three EMT-associated genes miR-200c/141, miR-200b/a/429 and CDH1 in single cells...
January 9, 2017: Oncogene
https://www.readbyqxmd.com/read/28067470/form-matters-stable-helix-foldamers-preferentially-target-human-monocytes-and-granulocytes
#11
Claudia Tomasini, Nicola Zanna, Lorenzo Milli, Benedetta Del Secco, Andrea Cornia, Regina Tavano, Emanuele Papini, Giulia Malachin
Some hybrid foldamers of different length all containing the D-Oxd moiety alternated with a L-amino acid (L-Val, L-Lys or L-Ala) have been prepared to study their preferred conformations and to evaluate their biological activity. Surprisingly, only the longer oligomers containing L-Ala fold into well-established helices, while all the other oligomers give partially unfolded turn structures. Nevertheless, they all show a good biocompatibility, with no detrimental effects up to 64 µM concentration. After equipping some selected foldamers with the fluorescent tag Rhodamine B, a quantitative analysis was obtained by dose and time response analysis by FACS of human HeLa cells, and primary blood lymphocytes, granulocytes and monocytes...
January 9, 2017: ChemMedChem
https://www.readbyqxmd.com/read/28066995/molecular-galactose-galectin-association-in-neuroblastoma-cells-an-unconventional-tool-for-qualitative-quantitative-screening
#12
Fabio Pastorino, Mirco Ponzoni, Giuseppina Simone
Galectin decorates the cell membrane and forms an extracellular molecular association with galactoside units. Here, galactoside probes have been used to study galectin expression in neuroblastoma cells. The hypothesis behind this investigation has been that the molecular mechanisms by which glycans modulate neural metastatic cells involve a protein-carbohydrate association, galectin-galactose. Preliminary screening to validate the hypothesis has been performed with galactose moieties anchored to beads. The molecular association has been studied by FACS...
January 9, 2017: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/28064427/biochemical-and-molecular-study-of-carpobrotus-edulis-bioactive-properties-and-their-effects-on-dugesia-sicula-turbellaria-tricladida-regeneration
#13
Emna Meddeb, Mohamed Charni, Tesnime Ghazouani, Autilia Cozzolino, Florinda Fratianni, Faten Raboudi, Filomena Nazzaro, Sami Fattouch
The traditional medicinal properties of Carpobrotus edulis are well recognized, particularly in Tunisia where it is used for wound healing. Thus, in this study, biochemical and molecular properties of its leaves' bioactive aqueous-acetone extract were investigated. The total phenolic content (TPC) of the extract was estimated to be 184 ± 5 mg/100 g of fresh matter (FM). The qualitative and quantitative polyphenolic profile was determined by ultra performance liquid chromatography with diode array detection (UPLC-DAD) and showed that chlorogenic acid was the major compound (43...
January 7, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28063055/isolation-of-microglia-and-immune-infiltrates-from-mouse-and-primate-central-nervous-system
#14
Thais F Galatro, Ilia D Vainchtein, Nieske Brouwer, Erik W G M Boddeke, Bart J L Eggen
Microglia are the innate immune cells of the central nervous system (CNS) and play an important role in the maintenance of tissue homeostasis, providing neural support and neuroprotection. Microglia constantly survey their environment and quickly respond to homeostatic perturbations. Microglia are increasingly implicated in neuropathological and neurodegenerative conditions, such as Alzheimer's disease, Parkinson's disease, and glioma progression. Here, we describe a detailed isolation protocol for microglia and immune infiltrates, optimized for large amounts of post mortem tissue from human and rhesus macaque, as well as smaller tissue amounts from mouse brain and spinal cord, that yield a highly purified microglia population (up to 98 % purity)...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28062807/junctional-adhesion-molecule-a-expression-in-the-murine-epididymal-tract-and-accessory-organs-and-acquisition-by-maturing-sperm
#15
Kathie Z Wu, Kun Li, Deni S Galileo, Patricia A Martin-DeLeon
STUDY QUESTION: Is junctional adhesion molecule A (JAM-A), a sperm protein essential for normal motility, expressed in the murine post-testicular pathway and involved in sperm maturation? SUMMARY ANSWER: JAM-A is present in the prostate and seminal vesicles and in all three regions of the epididymis where it is secreted in epididymosomes in the luminal fluid and can be delivered to sperm in vitro. WHAT IS KNOWN ALREADY: JAM-A shares with the plasma membrane Ca(2+)ATPase 4 (PMCA4, the major Ca(2+) efflux pump in murine sperm) a common interacting partner, CASK (Ca(2+)/CaM-dependent serine kinase)...
January 6, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28061901/rbm5-inhibits-tumorigenesis-of-gliomas-through-inhibition-of-wnt-%C3%AE-catenin-signaling-and-induction-of-apoptosis
#16
Yuanpei Jiang, Hongling Sheng, Lei Meng, Hongsheng Yue, Bo Li, Aijun Zhang, Yanan Dong, Yuguang Liu
BACKGROUND: Gliomas are one of the most common malignant brain tumors and bring a big threat to human life as traditional therapy is unsatisfactory. RBM5 was a RNA-binding motif protein and was reported as a tumor suppressor. But the role of RBM5 in gliomas was unknown. METHODS: The mRNA level of RBM5 was determined in gliomas tissues and cell lines by real-time quantitative PCR (qRT-PCR) assay while the association of RBM5 expression with prognosis was analyzed by Kaplan-Meier method and compared by log-rank test...
January 6, 2017: World Journal of Surgical Oncology
https://www.readbyqxmd.com/read/28058014/anti-viral-role-of-toll-like-receptor-4-in-hepatitis-b-virus-infection-an-in-vitro-study
#17
Dipanwita Das, Neelakshi Sarkar, Isha Sengupta, Ananya Pal, Debraj Saha, Manikankana Bandopadhyay, Chandrima Das, Jimmy Narayan, Shivram Prasad Singh, Runu Chakravarty
AIM: Toll like receptors plays a significant anti-viral role in different infections. The aim of this study was to look into the role of toll like receptor 4 (TLR4) in hepatitis B virus (HBV) infection. METHODS: Real time PCR was used to analyze the transcription of TLR4 signaling molecules, cell cycle regulators and HBV DNA viral load after triggering the HepG2.2.15 cells with TLR4 specific ligand. Nuclear factor (NF)-κB translocation on TLR4 activation was analyzed using microscopic techniques...
December 21, 2016: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28057919/isolation-of-cell-type-specific-apoptotic-bodies-by-fluorescence-activated-cell-sorting
#18
Georgia K Atkin-Smith, Stephanie Paone, Damien J Zanker, Mubing Duan, Than K Phan, Weisan Chen, Mark D Hulett, Ivan K H Poon
Apoptotic bodies (ApoBDs) are membrane-bound extracellular vesicles that can mediate intercellular communication in physiological and pathological settings. By combining recently developed analytical strategies with fluorescence-activated cell sorting (FACS), we have developed a method that enables the isolation of ApoBDs from cultured cells to 99% purity. In addition, this approach also enables the identification and isolation of cell type-specific ApoBDs from tissue, bodily fluid and blood-derived samples...
January 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28056449/transformation-of-aminopyrine-in-the-presence-of-free-available-chlorine-kinetics-products-and-reaction-pathways
#19
Mei-Quan Cai, Li Feng, Li-Qiu Zhang
Aminopyrine (AMP) has been frequently detected in the aquatic environment. In this study, the transformation mechanism of AMP by free available chlorine (FAC) oxidation was investigated. The results showed that FAC reacted with AMP rapidly, and a 74% elimination was achieved for 1.30 μM AMP after 2 min at 14.08 μM FAC dose. AMP chlorination was strongly pH-dependent, and its reaction included second- and third-order kinetic processes. Three active FAC species, including chlorine monoxide (Cl2O), molecular chlorine (Cl2), and hypochlorous acid (HOCl), were observed to contribute to AMP degradation...
December 26, 2016: Chemosphere
https://www.readbyqxmd.com/read/28055304/synergistic-effect-of-cytokine-induced-killer-cell-with-valproate-inhibits-growth-of-hepatocellular-carcinoma-cell-in-a-mouse-model
#20
Dong Hyeon Lee, Joon Yeul Nam, Young Chang, Hyeki Cho, Seong Hee Kang, Young Youn Cho, EunJu Cho, Jeong-Hoon Lee, Su Jong Yu, Yoon Jun Kim, Jung-Hwan Yoon
OBJECTIVE: Long-term prognosis of hepatocellular carcinoma (HCC) remains poor owing to the lack of treatment options for advanced HCC. Cytokine-induced killer (CIK) cells are ex vivo expanded T lymphocytes expressing both NK- and T-cell markers. CIK cell therapy alone is insufficient for treating advanced HCC. Thus, this study aimed to determine whether treatment with CIK cells combined with valproic acid (VPA) could provide a synergistic effect to inhibit tumor growth in a mouse model of HCC...
January 5, 2017: Cancer Biology & Therapy
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