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Bin Sun, Sandra Nurttila, Joost Reek
We present here the synthesis of chiral BINOL derived bisamine and bispyridine-aldehyde building blocks that can be used for the self-assembly of novel chiral FeII2L3 cages when mixed with an Fe(II) precursor. The properties of a series of chiral cages were studied by NMR, CSI-MS and CD spectroscopy and molecular modelling. Upon formation of the M2L3 cages the iron corners can adopt various isomeric forms: mer, fac-Δ or fac-Λ. We found that the coordination geometry around the metal centers in R-Cage 1 and 2, are influenced by the chiral BINOL backbone only to a limited extent, as a mixture of cages are formed with fac- and mer- configurations at the iron corners...
July 19, 2018: Chemistry: a European Journal
Yolanda Schaerli
High-throughput screening of a DNA library expressed in a bacterial population for identifying potentially rare members displaying a property of interest is a crucial step for success in many experiments such as directed evolution of proteins and synthetic circuits and deep mutational scanning to identify gain- or loss-of-function mutants. Here, I describe a protocol for high-throughput screening of bacterial ( E. coli ) microcolonies in gel beads. Single cells are encapsulated into monodisperse water-in-oil emulsion droplets produced with a microfluidic device...
July 5, 2018: Bio-protocol
Shuang Wang, Huan Yang, Xin Chen, Zheng Jiang
AIM: To detect the function and mechanism of SOX15 in colorectal cancer. MATERIALS & METHODS: The expression of SOX15 in colorectal cancer (CRC) cells and tissues was detected by real-time PCR, quantitative real-time PCR and immunohistochemistry. The overexpression of SOX15 in cells was tested by western blot, CCK-8, FACS analysis, transwell and clone assay. Moreover, SOX15 regulated Wnt pathway investigated by western blot. RESULTS: We found that SOX15 has a low-expression in CRC cells and tissues...
July 18, 2018: Future Oncology
Isabela B Dos Santos, Danielle A M da Silva, Fabiana A C R Paz, Daniel M Garcia, Adriana K Carmona, Daniela Teixeira, Ieda M Longo-Maugéri, Simone Katz, Clara L Barbiéri
The present study focused on the activity of the palladacycle complex DPPE 1.1 on Leishmania (Leishmania) amazonensis . Promastigotes of L. (L.) amazonensis were destroyed in vitro by nanomolar concentrations of DPPE 1.1, whereas intracellular amastigotes were killed at drug concentrations fivefold less toxic than those harmful to macrophages. L. (L.) amazonensis -infected BALB/c mice were treated by intralesional injection of DPPE 1.1. Animals treated with 3.5 and 7.0 mg/kg of DPPE 1.1 showed a significant decrease of foot lesion sizes and a parasite load reduction of 93 and 99%, respectively, when compared to untreated controls...
2018: Frontiers in Microbiology
Patrycja Tudrej, Magdalena Olbryt, Ewa Zembala-Nożyńska, Katarzyna A Kujawa, Alexander J Cortez, Anna Fiszer-Kierzkowska, Wojciech Pigłowski, Barbara Nikiel, Magdalena Głowala-Kosińska, Aleksandra Bartkowska-Chrobok, Andrzej Smagur, Wojciech Fidyk, Katarzyna M Lisowska
High-grade serous ovarian carcinoma (HGSOC) is the most frequent histological type of ovarian cancer and the one with worst prognosis. Unfortunately, the majority of established ovarian cancer cell lines which are used in the research have unclear histological origin and probably do not represent HGSOC. Thus, new and reliable models of HGSOC are needed. Ascitic fluid from a patient with recurrent HGSOC was used to establish a stable cancer cell line. Cells were characterized by cytogenetic karyotyping and short tandem repeat (STR) profiling...
July 17, 2018: International Journal of Molecular Sciences
Yuki Hiradate, Eita Sasaki, Haruka Momose, Hideki Asanuma, Keiko Furuhata, Mamiko Takai, Taiki Aoshi, Hiroshi Yamada, Ken J Ishii, Kentaro Tanemura, Takuo Mizukami, Isao Hamaguchi
Recently, many vaccine adjuvants have been developed; however, most of the newly developed adjuvants have been dropped out of preclinical and clinical trials owing to their unexpected toxicity. Thus, the development of highly quantitative and comparable screening methods for evaluating adjuvant safety is needed. In a previous study, we identified specific biomarkers for evaluating the safety of an intranasal influenza vaccine with CpG K3 adjuvant by comparing biomarker expression. We hypothesized that these biomarkers might be useful for screening newly developed adjuvant safety...
July 13, 2018: Biologicals: Journal of the International Association of Biological Standardization
Xuehua Liu, Junhua Zuo, Yuan Fang, Jing Wen, Feihong Deng, Hui Zhong, Bo Jiang, Jide Wang, Biao Nie
The drug resistance of cancer remains a major obstacle to successful chemotherapy. New strategies for improving chemotherapeutic efficacy are urgently required. Recent studies have indicated that LIPC plays a role in promoting the liver metastasis of colorectal cancer. In the present study, we aimed to investigate the effects of LIPC on theproliferation and clone formation of colorectal cancer-derived cells, and chemoresistance in hepatoblastoma-derived HepG2 cells. The activity and expression of LIPC were determined in the cell lines by RT-qPCR and western blot analysis...
July 4, 2018: International Journal of Molecular Medicine
Diana M Norden, Timothy D Faw, Daniel B McKim, Rochelle J Deibert, Lesley C Fisher, John F Sheridan, Jonathan Phillip Godbout, D Michele Basso
Spinal cord injury (SCI) produces a toxic inflammatory microenvironment that negatively affects plasticity and recovery. Recently, we showed glial activation and peripheral myeloid cell infiltration extending beyond the epicenter through the remote lumbar cord after thoracic SCI. The presence and role of infiltrating monocytes is important, especially in the lumbar cord where locomotor central pattern generators are housed. Therefore, we compared the inflammatory profile of resident microglia and peripheral myeloid cells after SCI...
July 17, 2018: Journal of Neurotrauma
Kornkamon Lertsuwan, Ketsaraporn Nammultriputtar, Supanan Nanthawuttiphan, Supathra Phoaubon, Jomnarong Lertsuwan, Jirawan Thongbunchoo, Kannikar Wongdee, Narattaphol Charoenphandhu
The association between iron overload and osteoporosis has been found in many diseases, such as hemochromatosis, β-thalassemia and sickle cell anemia with multiple blood transfusion. One of the contributing factors is iron toxicity to osteoblasts. Some studies showed the negative effects of iron on osteoblasts; however, the effects of two biological available iron species, i.e., ferric and ferrous, on osteoblasts are elusive. Since most intracellular ionized iron is ferric, osteoblasts was hypothesized to be more responsive to ferric iron...
July 16, 2018: Biometals: An International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
William A Teeter, Bianca M Conti, Phil J Wasicek, Jonathan J Morrison, Dawn Parsell, Bryan Gamble, Melanie R Hoehn, Thomas M Scalea, Samuel M Galvagno
BACKGROUND: There are numerous studies in the cardiovascular literature that have employed transesophageal echocardiography (TEE) in swine models, but data regarding the use of basic TEE in swine models is limited. The primary aim of this study is to describe an echocardiographic method that can be used with relative ease to qualitatively assess cardiovascular function in a porcine hemorrhagic shock model using resuscitative endovascular balloon occlusion of the aorta (REBOA). METHODS: Multiplane basic TEE exams were performed in 15 during an experimental hemorrhage model using REBOA...
July 16, 2018: Cardiovascular Ultrasound
Roopali Khanna, Arvind S Raghuvanshi, Sudeep Kumar, Naveen Garg, Satyendra Tewari, Aditya Kapoor, Pravin K Goel
BACKGROUND: Right ventricular (RV) function has prognostic value in terms of survival and symptoms in patients with mitral stenosis (MS). The aim of the study was to assess RV function by strain analysis in the patients of mitral stenosis and the effect of percutaneous transvenous mitral commisurotomy (PTMC) on it. METHODS: Eighty patients of severe mitral stenosis without overt right heart failure and normal sinus rhythm undergoing PTMC were included. Conventional echocardiography and RV function by TDI-derived longitudinal strain and strain rate were assessed prior and 24 hours post PTMC and compared with 40 healthy age-matched controls...
July 16, 2018: Echocardiography
Xin Li, Wenyi Ma, Xiaojie Wang, Yunzhe Ci, Yina Zhao
BACKGROUND: Annexin A5 (ANXA5) is a kind of Ca2+-dependent phospholipid binding protein which is involved in cell membrane dynamics and organization. Recent data showed that ANXA5 might involve in tumorigenesis. OBJECTIVE: To explore what role ANXA5 play in human uterine cervical carcinoma. MATERIALS AND METHODS: In this study, a recombined ANXA5 plasmid was constructed and uterine cervical carcinoma cell lines HeLa and SiHa were transfected with it...
June 27, 2018: Cancer Biomarkers: Section A of Disease Markers
Yehfang Hu, Kevin Maisey, Parasuraman Aiya Subramani, Fuguo Liu, Camila Flores-Kossack, Mónica Imarai, Christopher J Secombes, Tiehui Wang
Rapid and high quality preparation of peripheral blood leucocytes (PBL) is important in fish immunology research and in particular for fish vaccine development, where multiple immune parameters can be monitored on the same fish over time. Fish PBL are currently prepared by density separation using Percoll or Hispaque-1.077, which is time consuming, costly and prone to erythrocyte contamination. We present here a modified PBL preparation method that includes a 20 s hypotonic lysis of erythrocytes and a subsequent separation of PBL from cell debris by a cell strainer...
July 17, 2018: Developmental and Comparative Immunology
Anna-Maria Husa, Maria Regina Strobl, Agata Strajeriu, Matthias Wieser, Sabine Strehl, Klaus Fortschegger
Hematopoietic stem and progenitor cells (HSPCs) derived from human induced pluripotent stem cells (hiPSCs) hold great promise for disease modeling, drug screens and eventually cell therapy approaches. During in vitro differentiation of hiPSC into hemato-endothelial progenitors, the emergence of CD34-positive cells indicates a critical step of lineage specification. To facilitate the monitoring of hematopoietic differentiation of hiPSCs, we established fluorescent reporter cells for the stem and progenitor cell marker CD34...
July 16, 2018: Stem Cells and Development
Kim Le, Christopher Tan, Shivani Gupta, Trupti Guhan, Hedieh Barkhordarian, Jonathan Lull, Jennitte Stevens, Trent Munro
Generating a highly productive cell line is resource intensive and typically involves long timelines, due to the need to screen large numbers of candidates in protein production studies. This has led to miniaturization and automation strategies to allow for reductions in resources and higher throughput. Current approaches rely on the use of standard cell culture vessels and bulky liquid handling equipment. New nanofludic technologies offer novel solutions to surpass these limits, further miniaturizing cell culture volumes (105 times smaller) by growing cells on custom nanofluidic chips...
July 16, 2018: Biotechnology Progress
Shuang-Li Zhang, Bao-Lin Li, Wei Li, Ming Lu, Lin-Ying Ni, Hui-Li Ma, Qing-Gang Meng
BACKGROUND The aim of this study was investigate the effects of the sesquiterpene lactone, ludartin, on cell proliferation, cell migration, apoptosis, and the cell cycle in osteosarcoma cell lines, compared with a normal osteoblast cell line. MATERIAL AND METHODS Osteosarcoma cell lines, MG-63 Saos-2 U-2OS, T1-73 143B, and HOS, and normal hFOB 1.19 osteoblasts, were cultured and treated with increasing doses of ludartin, The MTT colorimetric assay was used to measure cell metabolic activity and viability. Apoptosis was studied by fluorescence-activated cell sorting (FACS) using 4',6-diamidino-2-phenylindole (DAPI) nuclear staining and Annexin-V/propidium iodide (PI) staining...
July 16, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Aurora Mazzeo, Elena Beltramo, Tatiana Lopatina, Chiara Gai, Marina Trento, Massimo Porta
Diabetic retinopathy is a sight-threatening complication of diabetes, characterized by loss of retinal pericytes and abnormal angiogenesis. We previously demonstrated that extracellular vesicles (EVs) derived from mesenchymal stem cells cultured in diabetic-like conditions are able to enter the pericytes, causing their detachment and migration, and stimulating angiogenesis in vitro. The purpose of this work was the molecular and functional characterization of EVs derived from diabetic subjects with or without diabetic retinopathy, compared with healthy controls...
July 3, 2018: Experimental Eye Research
Yasunori Enomoto, Sayomi Matsushima, Kiyoshi Shibata, Yoichiro Aoshima, Haruna Yagi, Shiori Meguro, Hideya Kawasaki, Isao Kosugi, Tomoyuki Fujisawa, Noriyuki Enomoto, Naoki Inui, Yutaro Nakamura, Takafumi Suda, Toshihide Iwashita
Although differentiation of lung fibroblasts into α-smooth muscle actin (αSMA)-positive myofibroblasts is important in the progression of idiopathic pulmonary fibrosis (IPF), few biomarkers reflecting the fibrotic process have been discovered. We performed microarray analyses between FACS-sorted steady-state fibroblasts (lineage [CD45, TER-119, CD324, CD31, LYVE-1, and CD146]-negative and PDGFRα-positive cells) from untreated mouse lungs and myofibroblasts (lineage-negative, Sca-1-negative, and CD49e-positive cells) from bleomycin-treated mouse lungs...
July 13, 2018: Clinical Science (1979-)
Paula Andrea Serna Ortega, Irma Saulle, Vincenzo Mercurio, Salomè Valentina Ibba, Elisa Maria Lori, Claudio Fenizia, Michela Masetti, Daria Trabattoni, Sergio Lo Caputo, Francesca Vichi, Francesco Mazzotta, Mario Clerici, Mara Biasin
BACKGROUND: Interleukin-21 (IL-21) modulates HIV-1 infection through the elicitation of different antiviral mechanisms, including Th17 lineage commitment and induction of microRNA (miR)-29, a miRNA endowed with anti-HIV activity. As miR-29 expression is significantly increased in HIV-1-exposed seronegative individuals (HESN), we investigated the role of miR-29/IL21 axis in the natural control of HIV-1 infection. METHODS: PBMCs isolated from 15 Italian sexually-exposed HESN and 15 HIV-unexposed healthy controls (HC) were in vitro infected with an R5-tropic HIV-1Ba-L strain...
July 12, 2018: AIDS
Yichao Mo, Longguang He, Zeru Lai, Zhiheng Wan, Qinshou Chen, Sibo Pan, Liangfu Li, Dasheng Li, Junwei Huang, Fan Xue, Siyao Che
BACKGROUND: The long non-coding RNAs (lncRNAs) have participated in the promotion of hepatocellular carcinoma (HCC) initiation and progression. Nevertheless, the biological role and underlying mechanism of LINC01287 in HCC has never been reported. METHODS: The TGCA database was used to explore the abnormal expression of lncRNAs in HCC. Real-time PCR and in situ hybridization assays were used to examine the expression of LINC01287 in HCC tissues. The clinicopathological characteristics of HCC patients in relation to LINC01287 expression were then analyzed...
July 13, 2018: Journal of Experimental & Clinical Cancer Research: CR
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