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https://www.readbyqxmd.com/read/27920923/mer-tri-chlorido-tris-tetra-hydro-thio-phene-%C3%AE%C2%BAs-iridium-iii-preparation-and-comparison-with-other-mer-tri-chlorido-tris-tetra-hydro-thio-phene-%C3%AE%C2%BAs-metal-complexes
#1
Loren C Brown, Christine M DuChane, Joseph S Merola
The title complex, [IrCl3(C4H8S)3], was prepared according to a literature method. A suitable crystal was obtained by diffusion of pentane into a di-chloro-methane solution and analyzed by single-crystal X-ray diffraction at 100 K. The title complex is isotypic with mer-tri-chlorido-tris-(tetra-hydro-thio-phene-κS)rhodium(III). However, the orientation of the tetra-hydro-thio-phene rings is different from an earlier report of mer-tri-chlorido-tris-(tetra-hydro-thio-phene-κS)iridium(III) deposited in the Cambridge Structural Database...
September 1, 2016: Acta Crystallographica. Section E, Crystallographic Communications
https://www.readbyqxmd.com/read/27919837/ex-vivo-itreg-differentiation-revisited-convenient-alternatives-to-existing-strategies
#2
Billur Akkaya, Amanda H Holstein, Christopher Isaac, Mitra P Maz, Deborah D Glass, Ethan M Shevach, Munir Akkaya
Ex-vivo differentiation of regulatory T cells (Tregs) from naïve CD4(+) T-cells has been widely used in immunological research. Isolation of a highly pure naïve T cell population is the key factor that determines the efficiency of subsequent Treg differentiation. Currently, this step relies mostly on FACS sorting, which is often costly, time consuming, and inconvenient. Alternatively, magnetic separation of T-cells can be performed; yet, available protocols fail to reach sort level purity and consequently result in low Treg differentiation efficiency...
December 2, 2016: Journal of Immunological Methods
https://www.readbyqxmd.com/read/27919774/understanding-facial-expressions-of-pain-in-patients-with-depression
#3
Stefan Lautenbacher, Karl-Juergen Bär, Patricia Eisold, Miriam Kunz
: Although depression is associated with more clinical pain complaints, psychophysical data sometimes point to hypoalgesic alterations. Studying the more reflex-like facial expression of pain in patients with depression may offer a new perspective. Facial and psychophysical responses to non-painful and painful heat stimuli were studied in 23 patients with major depressive disorder (MDD) and 23 matched controls. As psychophysical data, pain thresholds, tolerance thresholds and self-report were assessed...
December 2, 2016: Journal of Pain: Official Journal of the American Pain Society
https://www.readbyqxmd.com/read/27917231/magnetotail-energy-dissipation-during-an-auroral-substorm
#4
E V Panov, W Baumjohann, R A Wolf, R Nakamura, V Angelopoulos, J M Weygand, M V Kubyshkina
Violent releases of space plasma energy from the Earth's magnetotail during substorms produce strong electric currents and bright aurora. But what modulates these currents and aurora and controls dissipation of the energy released in the ionosphere? Using data from the THEMIS fleet of satellites and ground-based imagers and magnetometers, we show that plasma energy dissipation is controlled by field-aligned currents (FACs) produced and modulated during magnetotail topology change and oscillatory braking of fast plasma jets at 10-14 Earth radii in the nightside magnetosphere...
December 2016: Nature Physics
https://www.readbyqxmd.com/read/27917009/induced-expression-of-cancer-stem-cell-markers-aldh1a3-and-sox-2-in-hierarchical-reconstitution-of-apoptosis-resistant-human-breast-cancer-cells
#5
Karin Kashii-Magaribuchi, Rie Takeuchi, Yuko Haisa, Akemi Sakamoto, Aimi Itoh, Yuki Izawa, Miyuki Isa, Mayu Fukuzawa, Motonobu Murakami, Rei Takahashi
We established an experimental system that can induce p53-dependent apoptosis by doxycycline treatment to analyze characteristics of the apoptosis-resistant cancer cell subpopulation in the human breast cancer cell line HCC1937. Expression patterns of the stem cell markers, ALDH1A3 and Sox-2, the luminal differentiation marker, GATA3 and the proliferation index marker, Ki-67 were analyzed using immunostaining and fluorescence-activated cell sorting (FACS). After doxycycline treatment, the number of viable cells was gradually decreased over seven days in a time-dependent manner due to p53-induced apoptosis; however, the number of smaller-sized ALDH1A3(+) cells assessed by immunostaining increased sharply after 1 day of doxycycline treatment, suggesting their apoptosis-resistant nature...
November 1, 2016: Acta Histochemica et Cytochemica
https://www.readbyqxmd.com/read/27914988/imaging-of-macrophage-dynamics-with-optical-coherence-tomography-in-anterior-ischemic-optic-neuropathy
#6
Despina Kokona, Nathanael U Häner, Andreas Ebneter, Martin S Zinkernagel
Anterior ischemic optic neuropathy (AION) is a relatively common cause of visual loss and results from hypoperfusion of the small arteries of the anterior portion of the optic nerve. AION is the leading cause of sudden optic nerve related vision loss with approximately 10 cases per 100'000 in the population over 50 years. To date there is no established treatment for AION and therefore a better understanding of the events occurring at the level of the optic nerve head (ONH) would be important to design future therapeutic strategies...
November 30, 2016: Experimental Eye Research
https://www.readbyqxmd.com/read/27914920/can-lowering-the-guidance-force-of-robot-assisted-gait-training-induce-a-sufficient-metabolic-demand-in-subacute-dependent-ambulatory-stroke-patients
#7
So Young Lee, Eun Young Han, Bo Ryun Kim, Min Ho Chun, Yong Ki Lee
OBJECTIVE: To assess the effects of guidance force (GF) and gait speed (GS) on cardiorespiratory responses and energy cost in subacute dependent ambulatory stroke patients. DESIGN: Cross sectional study. SETTING: University rehabilitation hospital. PARTICIPANTS: Ten subacute stroke patients (mean age: 64.50±19.20), who were dependent ambulators (functional ambulation category [FAC] ≤2). INTERVENTIONS: Patients participated in cardiorespiratory tests during robot-assisted gait training...
November 30, 2016: Archives of Physical Medicine and Rehabilitation
https://www.readbyqxmd.com/read/27914650/towards-upright-pedalling-to-drive-recovery-in-people-who-cannot-walk-in-the-first-weeks-after-stroke-movement-patterns-and-measurement
#8
Nicola J Hancock, Lee Shepstone, Philip Rowe, Phyo K Myint, Valerie M Pomeroy
OBJECTIVES: To examine whether people who are within 31days of stroke onset are able to produce controlled lower limb movement, and phasic activity in antagonistic lower limb muscle groups, during Upright Pedalling (UP). DESIGN: Observational study. SETTING: Acute stroke unit within a University Hospital. PARTICIPANTS: Eight adults between 3 and 30days from stroke onset, with unilateral lower limb paresis and unable to walk without assistance...
November 15, 2016: Physiotherapy
https://www.readbyqxmd.com/read/27909710/intracellular-distribution-and-stability-of-a-luminescent-rhenium-i-tricarbonyl-tetrazolato-complex-using-epifluorescence-microscopy-in-conjunction-with-x-ray-fluorescence-imaging
#9
J L Wedding, H H Harris, C A Bader, S E Plush, R Mak, M Massi, D A Brooks, B Lai, S Vogt, M V Werrett, P V Simpson, B W Skelton, S Stagni
Optical epifluorescence microscopy was used in conjunction with X-ray fluorescence imaging to monitor the stability and intracellular distribution of the luminescent rhenium(i) complex fac-[Re(CO)3(phen)L], where phen = 1,10-phenathroline and L = 5-(4-iodophenyl)tetrazolato, in 22Rv1 cells. The rhenium complex showed no signs of ancillary ligand dissociation, a conclusion based on data obtained via X-ray fluorescence imaging aligning iodine and rhenium distributions. A diffuse reticular localisation was detected for the complex in the nuclear/perinuclear region of cells, by either optical or X-ray fluorescence imaging techniques...
December 2, 2016: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/27906115/established-cell-surface-markers-efficiently-isolate-highly-overlapping-populations-of-skeletal-muscle-satellite-cells-by-fluorescence-activated-cell-sorting
#10
Claire C Maesner, Albert E Almada, Amy J Wagers
BACKGROUND: Fluorescent-activated cell sorting (FACS) has enabled the direct isolation of highly enriched skeletal muscle stem cell, or satellite cell, populations from postnatal tissue. Several distinct surface marker panels containing different positively selecting surface antigens have been used to distinguish muscle satellite cells from other non-myogenic cell types. Because functional and transcriptional heterogeneity is known to exist within the satellite cell population, a direct comparison of results obtained in different laboratories has been complicated by a lack of clarity as to whether commonly utilized surface marker combinations select for distinct or overlapping subsets of the satellite cell pool...
November 8, 2016: Skeletal Muscle
https://www.readbyqxmd.com/read/27904907/the-impact-of-cd4-cd28-null-t-lymphocytes-on-atrial-fibrillation-and-mortality-in-patients-with-chronic-heart-failure
#11
Patrick Sulzgruber, Lorenz Koller, Max-Paul Winter, Bernhard Richter, Steffen Blum, Magdalena Korpak, Martin Hülsmann, Georg Goliasch, Johann Wojta, Alexander Niessner
Atrial fibrillation (AF) represents the most common cardiac arrhythmia. Especially in patients with chronic heart failure (CHF) the development of AF represents a severe complication resulting in haemodynamic deterioration. While pro-inflammatory cytokines proved to have a pivotal role in the development and progression of both AF and CHF, less attention has been paid to the cellular immunity. Therefore we prospectively enrolled 112 patients with CHF and performed fluorescein-activated cell sorting (FACS). Patients were stratified in two subgroups according to patients presenting with AF (n=56) and patients free of AF (n=56)...
December 1, 2016: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/27898902/effect-of-ferric-ammonium-citrate-in-feedlot-diets-with-varying-dried-distillers-grains-inclusion-on-ruminal-hydrogen-sulfide-concentrations-and-steer-growth
#12
D J Pogge, M E Drewnoski, D Snider, W K Rumbeiha, S L Hansen
Angus-cross steers ( = 128) were used to examine the effects of supplementing ferric ammonium citrate (FAC; 300 mg ferric Fe/kg DM) to diets of 20, 40, or 60% dried distillers' grains plus solubles (DDGS) on growth performance, liver mineral and ruminal hydrogen sulfide (HS) concentrations, and carcass traits of finishing steers. Steers were blocked by initial BW (436 ± 10.6 kg) into pens of 4 and randomly assigned to 1 of 6 treatments ( = 5 or 6 pens per treatment) including a 20, 40, or 60% DDGS inclusion diet with (+) or without (-) 300 mg Fe/kg DM from FAC...
September 2016: Journal of Animal Science
https://www.readbyqxmd.com/read/27898853/cookery-method-and-endpoint-temperature-can-affect-the-warner-bratzler-shear-force-cooking-loss-and-internal-cooked-color-of-beef-semimembranosus-and-infraspinatus-steaks
#13
J W S Yancey, J K Apple, M D Wharton
Steaks from USDA Select inside rounds (Exp. 1) and shoulder clods (Exp. 2) were used to test the interactive effect of cookery method and endpoint temperature on Warner-Bratzler shear force (WBSF) and internal cooked color. Pairs of 2.5-cm-thick semimembranosus (SM) or infraspinatus (INF) steaks ( = 360/muscle) were cut from each subprimal, labeled, vacuum packaged, and frozen at -30°C in the dark for approximately 60 d before being cooked to 65.5, 71.1, or 76.6°C using 1) a forced-air convection oven (FAC); 2) a forced-air impingement oven (IMP); 3) a gas-fired, open-hearth charbroiler (CHAR); 4) an electric countertop griddle (GRID); or 5) a clam-shell grill (CLAM)...
October 2016: Journal of Animal Science
https://www.readbyqxmd.com/read/27896136/attention-deficit-hyperactivity-disorder-suffers-from-mitochondrial-dysfunction
#14
Poonam Verma, Alpana Singh, Dominic Ngima Nthenge-Ngumbau, Usha Rajamma, Swagata Sinha, Kanchan Mukhopadhyay, Kochupurackal P Mohanakumar
BACKGROUND: Pathophysiology of attention-deficit hyperactivity disorder (ADHD) is not known, and therefore the present study investigated mitochondrial defects, if any in cybrids created from patients and control population. METHODS: To investigate mitochondrial pathology in ADHD, cybrids cell lines were created from ADHD probands and controls by fusing their platelets with ρ(0)-cells prepared from SH-SY5Y neuroblastoma cell line. Cellular respiration, oxidative stress, mitochondrial membrane potential and morphology were evaluated employing oxygraph, mitochondria-specific fluorescence staining and evaluation by FACS, and immunocytochemistry...
December 2016: BBA Clinical
https://www.readbyqxmd.com/read/27895656/meselect-a-rapid-and-effective-method-for-the-separation-of-the-main-leaf-tissue-types
#15
Julia Svozil, Wilhelm Gruissem, Katja Baerenfaller
Individual tissues of complex eukaryotic organisms have specific gene expression programs that control their functions. Therefore, tissue-specific molecular information is required to increase our understanding of tissue-specific processes. Established methods in plants to obtain specific tissues or cell types from their organ or tissue context typically require the enzymatic degradation of cell walls followed by fluorescence-activated cell sorting (FACS) using plants engineered for localized expression of green fluorescent protein...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27895398/dysregulation-of-innate-immunity-in-ulcerative-colitis-patients-who-fail-anti-tumor-necrosis-factor-therapy
#16
Angela C Baird, Dominic Mallon, Graham Radford-Smith, Julien Boyer, Thierry Piche, Susan L Prescott, Ian C Lawrance, Meri K Tulic
AIM: To study the innate immune function in ulcerative colitis (UC) patients who fail to respond to anti-tumor necrosis factor (TNF) therapy. METHODS: Effects of anti-TNF therapy, inflammation and medications on innate immune function were assessed by measuring peripheral blood mononuclear cell (PBMC) cytokine expression from 18 inflammatory bowel disease patients pre- and 3 mo post-anti-TNF therapy. Toll-like receptor (TLR) expression and cytokine production post TLR stimulation was assessed in UC "responders" (n = 12) and "non-responders" (n = 12) and compared to healthy controls (n = 12)...
November 7, 2016: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/27893093/ligation-of-cd40-in-human-m%C3%A3-ller-cells-induces-p2x7-receptor-dependent-death-of-retinal-endothelial-cells
#17
Jose-Andres C Portillo, Yalitza Lopez Corcino, George R Dubyak, Timothy S Kern, Shigemi Matsuyama, Carlos S Subauste
Purpose: Cluster of differentiation 40 (CD40) is required for retinal capillary degeneration in diabetic mice, a process mediated by the retinal endothelial cells (REC) death. However, CD40 activates prosurvival signals in endothelial cells. The purpose of this study was to identify a mechanism by which CD40 triggers programmed cell death (PCD) of RECs and address this paradox. Methods: Human RECs and Müller cells were incubated with CD154 and L-N6-(1-Iminoethyl)lysine (L-Nil, nitric oxide synthase 2 inhibitor), α-lipoic acid (inhibitor of oxidative stress), anti-Fas ligand antibody, or A-438079 (P2X7 adenosine triphosphate [ATP] receptor inhibitor)...
November 1, 2016: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/27891685/the-dna-aptamer-binds-stemness-enriched-cancer-cells-in-pancreatic-cancer
#18
Yoon-Jin Kim, Hee Seung Lee, Dawoon E Jung, Jeong Mi Kim, Si Young Song
Pancreatic cancer remains one of the most common and lethal cancers. Most patients (80%) present with inoperable advanced pancreatic cancer at initial diagnosis, and their early diagnosis is a significant unmet challenge. Recent studies indicate that cancer, including pancreatic cancer, is initiated and propagated by cancer stem cells (CSCs). CSCs are responsible not only for the pathogenesis of cancer but also for the heterogeneity, malignant degree, anticancer therapy resistance, and recurrence of tumors...
November 28, 2016: Journal of Molecular Recognition: JMR
https://www.readbyqxmd.com/read/27886969/a-pilot-study-to-gauge-effectiveness-of-standardized-patient-scenarios-in-assessing-general-surgery-milestones
#19
REVIEW
Denise W Gee, Roy Phitayakorn, Avni Khatri, Kathryn Butler, John T Mullen, Emil R Petrusa
PURPOSE: Some General Surgery Milestones can be difficult to assess in traditional clinical settings and especially difficult to assess in junior residents. The purpose of this pilot study was to METHODS: A total of 9 categorical interns participated in a comprehensive, 4-module, SP scenario designed to evaluate and manage right upper quadrant pain. SP checklist scores (SP%) were converted to Milestone-equivalent scores for direct comparison (SP-C). Milestone scores were analyzed from 3 different sources: SP, faculty (FAC), and CCC...
November 2016: Journal of Surgical Education
https://www.readbyqxmd.com/read/27884072/the-biological-effect-of-asbestos-exposure-is-dependent-on-changes-in-iron-homeostasis
#20
Andrew J Ghio, Joleen M Soukup, Lisa A Dailey, Judy H Richards, Haiyan Tong
Functional groups on the surface of fibrous silicates can complex iron. We tested the postulate that (1) asbestos complexes and sequesters host cell iron resulting in a disruption of metal homeostasis and (2) this loss of essential metal results in an oxidative stress and biological effect in respiratory epithelial cells. Exposure of BEAS-2B cells to 50 μg/mL chrysotile resulted in diminished concentrations of mitochondrial iron. Preincubation of these cells with 200 μM ferric ammonium citrate (FAC) prevented significant mitochondrial iron loss following the same exposure...
November 24, 2016: Inhalation Toxicology
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