keyword
MENU ▼
Read by QxMD icon Read
search

Sucrase

keyword
https://www.readbyqxmd.com/read/29453261/molecular-and-functional-study-of-a-branching-sucrase-like-glucansucrase-reveals-an-evolutionary-intermediate-between-two-subfamilies-of-the-gh70-enzymes
#1
Minghui Yan, Bing-Hua Wang, Xiaofen Xu, Peng Chang, Feng Hang, Zhengjun Wu, Chunping You, Zhenmin Liu
Family GH70 glucansucrases (GSs) catalyze the synthesis of α-glucans from sucrose, a reaction widely seen in lactic acid bacteria (LAB). These enzymes have been implicated in many aspects of the microbial life. Products of GSs have great commercial value as food supplements and medical materials, therefore these enzymes have attracted much attention from both fields of science and industry. However, certain issues concerning the origin and evolution of GS are still to be addressed, although an increasing number of GH70 enzymes have been characterized...
February 16, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29414174/valorisation-of-food-and-beverage-waste-via-saccharification-for-sugars-recovery
#2
Tsz Him Kwan, Khai Lun Ong, Md Ariful Haque, Wing Hei Kwan, Sandeep Kulkarni, Carol Sze Ki Lin
Valorisation of mixed food and beverage (F&B) waste was studied for the recovery of sugars via saccharification. Glucoamylase and sucrase were employed to hydrolyse the starch and sucrose present in the mixed F&B waste because of the high cost-effectiveness for such recovery. The Michaelis-Menten kinetics model suggests that preservatives and additives in beverages did not inhibit glucoamylase and sucrase during saccharification. High levels of glucose (228.1 g L-1) and fructose (55.7 g L-1) were efficiently produced within 12 h at a solid-to-liquid ratio of 37...
January 31, 2018: Bioresource Technology
https://www.readbyqxmd.com/read/29408290/increased-prevalence-of-rare-sucrase-isomaltase-si-pathogenic-variants-in-irritable-bowel-syndrome-patients
#3
Koldo Garcia-Etxebarria, Tenghao Zheng, Ferdinando Bonfiglio, Luis Bujanda, Aldona Dlugosz, Greger Lindberg, Peter T Schmidt, Pontus Karling, Bodil Ohlsson, Magnus Simren, Susanna Walter, Gerardo Nardone, Rosario Cuomo, Paolo Usai-Satta, Francesca Galeazzi, Matteo Neri, Piero Portincasa, Massimo Bellini, Giovanni Barbara, Daisy Jonkers, Shanti Eswaran, William D Chey, Purna Kashyap, Lin Chang, Emeran A Mayer, Mira M Wouters, Guy Boeckxstaens, Michael Camilleri, Andre Franke, Mauro D'Amato
No abstract text is available yet for this article.
February 2, 2018: Clinical Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/29408078/enzyme-activity-and-microbial-biomass-availability-in-artificial-soils-on-rock-cut-slopes-restored-with-outside-soil-spray-seeding-osss-influence-of-topography-and-season
#4
Wenjuan Zhang, Li Ruirui, Xiaoyan Ai, Jiao Chen, Wennian Xu, Wei Li, Yingwei Ai
Large-scale railway construction has resulted in large areas of bare-cut-slope, and outside soil spray seeding (OSSS), a frequently used technique, has been adopted for slope restoration for many years. However, compared with natural slope soils, the quality of artificial soils on rock-cut slopes is low. Enzyme activity and microbial biomass are the main indices used for estimating soil quality; thus, our objective was to explore the influence of slope position, slope aspect, and season on two important factors that positively influence the plant growth capability in artificial soil...
January 31, 2018: Journal of Environmental Management
https://www.readbyqxmd.com/read/29373684/the-effects-of-dietary-sulfur-amino-acids-on-growth-performance-intestinal-morphology-enzyme-activity-and-nutrient-transporters-in-weaning-piglets
#5
E Y Zong, P F Huang, H S Yang, W Zhang, J Z Li, Y L Li, X Q Ding, X Xiong, Y L Yin
Early weaning results in intestinal dysfunction in piglets while sulfur amino acids (SAA) are involved in improving intestinal functions. We tested a hypothesis that dietary supplementation with SAA can improve intestinal functions of weaning piglets and analyzed the effects of different dietary SAA levels on intestinal functions. A total of 80 piglets (Duroc × Landrace × Yorkshire) were weaned at 21 d of age and randomly assigned to one of five diets that contained 0.53, 0.63, 0.74, 0.85, or 0.96% SAA, which corresponded to 70, 85, 100, 115 or, 130% of the SAA:Lys ratio recommended by the NRC (2012)...
January 24, 2018: Journal of Animal Science
https://www.readbyqxmd.com/read/29370981/enhanced-production-of-extracellular-inulinase-by-the-yeast-kluyveromyces-marxianus-in-xylose-catabolic-state
#6
Hisashi Hoshida, Kenta Kidera, Ryuta Takishita, Nobuhisa Fujioka, Taiki Fukagawa, Rinji Akada
The production of extracellular proteins by the thermotolerant yeast Kluyveromyces marxianus, which utilizes various sugars, was investigated using media containing sugars such as glucose, galactose, and xylose. SDS-PAGE analysis of culture supernatants revealed abundant production of an extracellular protein when cells were grown in xylose medium. The N-terminal sequence of the extracellular protein was identical to a part of the inulinase encoded by INU1 in the genome. Inulinase is an enzyme hydrolyzing β-2,1-fructosyl bond in inulin and sucrose and is not required for xylose assimilation...
January 19, 2018: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/29367163/recent-insights-in-microbial-exopolysaccharide-biosynthesis-and-engineering-strategies
#7
REVIEW
Jochen Schmid
The distinct biosynthesis pathways for microbial exopolysaccharide production provide different engineering strategies to tailor the chemical structures of the final polymers. This review focuses on the latest insights in the various pathways and identifies bottlenecks as well as promising targets for tailoring microbial polysaccharide production. The main engineering strategies includes the combinatorial assembly of glycosyltransferases and engineering of the Wzx and Wzy proteins for flipping of repeating units as well as polymerization...
January 17, 2018: Current Opinion in Biotechnology
https://www.readbyqxmd.com/read/29331942/sucrase-isomaltase-15phe-ibs-risk-variant-in-relation-to-dietary-carbohydrates-and-faecal-microbiota-composition
#8
Louise Thingholm, Malte Rühlemann, Jun Wang, Matthias Hübenthal, Wolfgang Lieb, Matthias Laudes, Andre Franke, Mauro D'Amato
No abstract text is available yet for this article.
January 13, 2018: Gut
https://www.readbyqxmd.com/read/29324356/inflammation-induced-er-stress-affects-absorptive-intestinal-epithelial-cells-function-and-integrity
#9
Sucheera Chotikatum, Hassan Y Naim, Nahed El-Najjar
Recent studies have linked impairment of intestinal epithelial function in inflammatory bowel disease to the disturbance of endoplasmic reticulum homeostasis (ER) in response to stress. Most studies are on goblet and Paneth cells, which are considered more susceptible to stress due to their role in the protection of intestinal epithelium against microbes and harmful substances. However, studies on the role of inflammation-induced ER stress in absorptive intestinal cells are scarce. In this study, we show, using Caco-2 cells as a model of intestinal epithelial barrier, that inducing ER stress using a cocktail mixture of pro-inflammatory mediators [TNFα (50ng/ml), MCP1 (50ng/ml), and IL-1β (25ng/ml)] as observed in IBD patients induces ER stress and leads to significant changes in key proteins of the apical (sucrase-isomaltase (SI), dipeptidyl-peptidase (DPPIV), and ezrin) and basolateral (E-cadherin, zonula occludens (ZO-1), and connexin-43) membranes...
January 8, 2018: International Immunopharmacology
https://www.readbyqxmd.com/read/29311037/relationships-among-dietary-intakes-and-persistent-gastrointestinal-symptoms-in-patients-receiving-enzyme-treatment-for-genetic-sucrase-isomaltase-deficiency
#10
Anne Boney, Heather E Elser, Heidi J Silver
BACKGROUND: Sucrose-isomaltase deficiency (SID) remains underdiagnosed. Absent or reduced enzyme activity promotes diarrhea, abdominal bloating, and flatulence from undigested and malabsorbed disaccharides. Frequency and severity of gastrointestinal symptoms may be associated with the type of carbohydrates consumed. OBJECTIVE: To characterize the dietary intakes of patients treated with sacrosidase (Sucraid; QOL Medical) for SID and determine relationships between type of carbohydrates, sacrosidase dose, and gastrointestinal symptoms...
January 5, 2018: Journal of the Academy of Nutrition and Dietetics
https://www.readbyqxmd.com/read/29198708/knockdown-of-laminin-%C3%AE-5-stimulates-intestinal-cell-differentiation
#11
Manon Lepage, Amira Seltana, Marie-Pier Thibault, Éric Tremblay, Jean-François Beaulieu
Interactions between cells and the extracellular matrix regulate a wide range of cell processes such as proliferation and differentiation. Laminins are major components of the basement membrane that actively participate in most biological functions via their interactions with a variety of specific cell receptors. The α5-containing laminins (LAMA5) are one of the three main types of laminins identified at the epithelial basal lamina in the adult intestine. The aim of the present study was to investigate the role of α5-containing laminins on intestinal cell proliferation and differentiation...
November 30, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29180238/glucose-homeostasis-in-the-euryhaline-crab-cytograpsus-angulatus-effects-of-the-salinity-in-the-amylase-maltase-and-sucrase-activities-in-the-hepatopancreas-and-in-the-carbohydrate-reserves-in-different-tissues
#12
Antonela Asaro, Roberto Alejandro Paggi, Juana Cristina Del Valle, Alejandra Antonia López Mañanes
We studied the existence, biochemical characteristics and response to different environmental salinities of amylase, maltase and sucrase activity in the intertidal euryhaline crab Cyrtograpsus angulatus (Dana, 1852) along with the response to distinct salinities of glycogen and free glucose content in storage organs. Amylase, maltase and sucrase activities were kept over a broad range of pH and temperature and exhibited Michaelis-Menten kinetics. Zymography showed the existence of two amylase forms in crabs exposed to 35 (osmoconformation) and low (6-10psu; hyper-regulation) or high (40psu) (hypo-regulation) salinities...
November 24, 2017: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29142393/investigation-of-antihyperglycaemic-activity-of-banana-musa-sp-var-nanjangud-rasa-bale-flower-in-normal-and-diabetic-rats
#13
Ramith Ramu, Prithvi S Shirahatti, S P Dhanabal, Farhan Zameer, B L Dhananjaya, M N Nagendra Prasad
Background: The vital enzymes of starch digestion and absorption are intestinal α-glucosidases and their inhibition improves postprandial hyperglycaemia, constituting an effective mode of therapy in diabetes. Objectives: The present study was designed to assess the inhibitory potential of ethanol extract of banana flower (EF) on mammalian α-glucosidases and its pharmacological effects on postprandial hyperglycaemia in normal and alloxan-induced diabetic rats. Materials and Methods: EF was evaluated for its inhibitory potential and mode of inhibition on mammalian α-glucosidases...
October 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/29053368/phytostabilization-potential-of-ornamental-plants-grown-in-soil-contaminated-with-cadmium
#14
Peng Zeng, Zhaohui Guo, Xia Cao, Xiyuan Xiao, Yanan Liu, Lei Shi
In a greenhouse experiment, five ornamental plants, Osmanthus fragrans (OF), Ligustrum vicaryi L. (LV), Cinnamomum camphora (CC), Loropetalum chinense var. rubrum (LC), and Euonymus japonicas cv. Aureo-mar (EJ), were studied for the ability to phytostabilization for Cd-contaminated soil. The results showed that these five ornamental plants can grow normally when the soil Cd content is less than 24.6 mg·kg(-1). Cd was mainly deposited in the roots of OF, LV, LC and EJ which have grown in Cd-contaminated soils, and the maximum Cd contents reached 15...
October 20, 2017: International Journal of Phytoremediation
https://www.readbyqxmd.com/read/29052391/-research-on-bacteria-microecology-in-root-rot-rhizosphere-soil-of-coptis-chinensis-produced-in-shizhu-city
#15
Xu-Hong Song, Yu Wang, Long-Yun Li, Jun Tan
Illumina Hiseq 2500 high-throughput sequencing platform was used to study the bacteria richness and diversity, the soil enzyme activities, nutrients in unplanted soil, root-rot and healthy rhizophere soil of Coptis chinensis for deeply discussing the mechanism of the root-rot of C. chinensis. The high-throughput sequencing result showed that the artificial cultivation effected the bacteria community richness and diversity. The bacteria community richness in healthy and diseased rhizosphere soil showed significant lower than that of in unplanted soil (P<0...
April 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29027951/effects-of-maternal-low-energy-diet-during-gestation-on-intestinal-morphology-disaccharidase-activity-and-immune-response-to-lipopolysaccharide-challenge-in-pig-offspring
#16
Yuling Chen, Daolin Mou, Liang Hu, Jie Zhen, Lianqiang Che, Zhengfeng Fang, Shengyu Xu, Yan Lin, Bin Feng, Jian Li, De Wu
Maternal nutrition during gestation is involved in the offspring's intestinal development and immunity. The aim of this study was to (1) determine the effects of maternal energy on intestinal digestion and absorption function in offspring, using pigs as a model; and (2) to evaluate the potential effect and mechanisms of maternal energy in modulating immune responses of lipopolysaccharide (LPS)-challenged piglets. After mating, thirty-six nine-parity sows (Landrace × Yorkshire), body weight (BW) (initial body weight 233...
October 13, 2017: Nutrients
https://www.readbyqxmd.com/read/28994704/characterization-of-mucosal-disaccharidases-from-human-intestine
#17
Mahdi Amiri, Hassan Y Naim
In this study, we used a brush border membrane (BBM) preparation from human small intestine to analyze the proportion and the activity of major intestinal disaccharidases, including sucrase-isomaltase (SI), maltase-glucoamylase (MGAM) and lactase-phlorizin hydrolase (LPH). SI, MGAM and LPH respectively constituted 8.2%, 2.7% and 1.4% of total BBM protein. The activity of SI and LPH decreased threefold after purification from the brush border membrane, which highlights the effect of membrane microdomains on the functional capacity of these enzymes...
October 10, 2017: Nutrients
https://www.readbyqxmd.com/read/28920455/a-new-4-arylflavan-from-the-pericarps-of-horsfieldia-motleyi-displaying-dual-inhibition-against-%C3%AE-glucosidase-and-free-radicals
#18
Rico Ramadhan, Irawan Wijaya Kusuma, Rudianto Amirta, Wisuttaya Worawalai, Preecha Phuwapraisirisan
In search for effective antidiabetic agents that simultaneously inhibit α-glucosidase and scavenge free radicals, Horsfieldia motleyi showed promising bioactivity according to the proposed criteria. Bioassay-guided isolation of pericarp extract yielded a new 4-arylflavan named myristinin G (6), whose gross structure and absolute configuration were verified by 2D NMR and electronic circular dichorism (ECD). Myristinin G (6) concomitantly inhibited α-glucosidases (IC50 107.0 and 126.9 μM) and free radicals (SC50 54...
September 18, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28914528/inhibition-of-human-and-rat-sucrase-and-maltase-activities-to-assess-antiglycemic-potential-optimization-of-the-assay-using-acarbose-and-polyphenols
#19
Alison Pyner, Hilda Nyambe-Silavwe, Gary Williamson
We optimized the assays used to measure inhibition of rat and human α-glucosidases (sucrase and maltase activities), intestinal enzymes which catalyze the final steps of carbohydrate digestion. Cell-free extracts from fully differentiated intestinal Caco-2/TC7 monolayers were shown to be a suitable source of sucrase-isomaltase, with the same sequence as human small intestine, and were compared to a rat intestinal extract. The kinetic conditions of the assay were optimized, including comparison of enzymatic and chromatographic methods to detect the monosaccharide products...
September 26, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28875556/melatonin-alleviates-weanling-stress-in-mice-involvement-of-intestinal-microbiota
#20
Wenkai Ren, Peng Wang, Jiameng Yan, Gang Liu, Benhua Zeng, Tarique Hussain, Can Peng, Jie Yin, Bie Tan, Tiejun Li, Hong Wei, Guoqiang Zhu, Russel J Reiter, Yulong Yin
Melatonin influences intestinal microbiota, and the pathogenesis of various diseases. This study was conducted to explore whether melatonin alleviates weanling stress through intestinal microbiota in a weanling mouse model. Melatonin supplementation in weanling mice (provided in the drinking water at a dosage of 0.2mg/ml for 2 weeks) significantly improved body weight gain (1.4±0.03 g/day in melatonin group vs. 1.2±0.06 g/day in control group) and intestinal morphology [i.e., villus length (258.1±12.2 um in melatonin group vs...
September 5, 2017: Journal of Pineal Research
keyword
keyword
18485
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"