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https://www.readbyqxmd.com/read/29241085/salting-out-assisted-liquid-liquid-extraction-coupled-with-hydrophilic-interaction-chromatography-for-the-determination-of-biguanides-in-biological-and-environmental-samples
#1
Anas Alshishani, Salizawati Muhamad Salhimi, Bahruddin Saad
A new salting-out assisted liquid-liquid extraction (SALLE) sample preparation method for the determination of the polar anti-diabetic biguanide drugs (metformin, buformin and phenformin) in blood plasma, urine and lake water samples were developed. The SALLE was performed by mixing samples (plasma (0.2mL), urine or lake water (1.0mL)) with acetonitrile (0.4mL for plasma, 0.5mL for urine or lake water), sodium hydroxide powder was then added for the phase separation. The effects of type of salting-out reagent, type of extraction solvent, volumes of acetonitrile and sample, amount of sodium hydroxide, vortexing and centrifugation times on the extraction efficiency were investigated...
December 9, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29240926/exocet-exonuclease-in-vitro-assembly-combined-with-recet-recombination-for-highly-efficient-direct-dna-cloning-from-complex-genomes
#2
Hailong Wang, Zhen Li, Ruonan Jia, Jia Yin, Aiying Li, Liqiu Xia, Yulong Yin, Rolf Müller, Jun Fu, A Francis Stewart, Youming Zhang
The exponentially increasing volumes of DNA sequence data highlight the need for new DNA cloning methods to explore the new information. Here, we describe 'ExoCET' (Exonuclease Combined with RecET recombination) to directly clone any chosen region from bacterial and mammalian genomes with nucleotide precision into operational plasmids. ExoCET combines in vitro exonuclease and annealing with the remarkable capacity of full length RecET homologous recombination (HR) to retrieve specified regions from genomic DNA preparations...
December 12, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29240905/coronary-autoregulation-and-assessment-of-stenosis-severity-without-pharmacological-vasodilation
#3
Guus A de Waard, Christopher M Cook, Niels van Royen, Justin E Davies
For identification of myocardial ischaemia by non-invasive imaging or invasive physiological assessment, administration of a vasodilating or positive inotropic agent is often considered indispensable to exhaust the cardiac compensation mechanisms. Indeed, microcirculatory dilatation is needed for assessment of myocardial perfusion or coronary blood flow. However, three different modalities exist that quantify myocardial ischaemia without recourse to pharmacological stress agents, using either myocardial contrast echocardiography, imaging of myocardial blood volume or invasive coronary pressure measurement with the instantaneous wave-free ratio (iFR)...
December 11, 2017: European Heart Journal
https://www.readbyqxmd.com/read/29240833/vagal-nerve-stimulation-triggers-widespread-responses-and-alters-large-scale-functional-connectivity-in-the-rat-brain
#4
Jiayue Cao, Kun-Han Lu, Terry L Powley, Zhongming Liu
Vagus nerve stimulation (VNS) is a therapy for epilepsy and depression. However, its efficacy varies and its mechanism remains unclear. Prior studies have used functional magnetic resonance imaging (fMRI) to map brain activations with VNS in human brains, but have reported inconsistent findings. The source of inconsistency is likely attributable to the complex temporal characteristics of VNS-evoked fMRI responses that cannot be fully explained by simplified response models in the conventional model-based analysis for activation mapping...
2017: PloS One
https://www.readbyqxmd.com/read/29240808/altered-cerebral-hemodyamics-and-cortical-thinning-in-asymptomatic-carotid-artery-stenosis
#5
Randolph S Marshall, Iris Asllani, Marykay A Pavol, Ying-Kuen Cheung, Ronald M Lazar
Cortical thinning is a potentially important biomarker, but the pathophysiology in cerebrovascular disease is unknown. We investigated the association between regional cortical blood flow and regional cortical thickness in patients with asymptomatic unilateral high-grade internal carotid artery disease without stroke. Twenty-nine patients underwent high resolution anatomical and single-delay, pseudocontinuous arterial spin labeling magnetic resonance imaging with partial volume correction to assess gray matter baseline flow...
2017: PloS One
https://www.readbyqxmd.com/read/29239982/postexercise-hypotension-and-heart-rate-variability-responses-subsequent-to-traditional-paired-set-and-superset-training-methods
#6
Gabriel A Paz, Eliseo Iglesias-Soler, Jeffrey M Willardson, Marianna de Freitas Maia, Humberto Miranda
The purpose of this study was to compare training volume, post-exercise hypotension (PEH) and heart rate variability (HRV) responses to different strength training methods. Thirteen trained men volunteered for this study. Three training methods were completed in a randomized design, which included: Traditional Set (TS) - three successive sets for the lying bench press (LBP), lat pulldown (LPD), incline 45° bench press (BP45), seated close-grip row (SCR), triceps extension (TE), and biceps curl (BC), with a 90 sec...
December 11, 2017: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/29239963/in-vitro-evaluation-of-a-novel-image-processing-device-to-estimate-surgical-blood-loss-in-suction-canisters
#7
Gerhardt Konig, Jonathan H Waters, Eric Hsieh, Bridget Philip, Vicki Ting, Gaurav Abbi, Mazyar Javidroozi, Griffeth W Tully, Gregg Adams
BACKGROUND: Clinicians are tasked with monitoring surgical blood loss. Unfortunately, there is no reliable method available to assure an accurate result. Most blood lost during surgery ends up on surgical sponges and within suction canisters. A novel Food and Drug Administration-cleared device (Triton system; Gauss Surgical, Inc, Los Altos, CA) to measure the amount of blood present on sponges using computer image analysis has been previously described. This study reports on performance of a complementary Food and Drug Administration-cleared device (Triton Canister System; Gauss Surgical, Inc, Los Altos, CA) that uses similar image analysis to measure the amount of blood in suction canisters...
December 11, 2017: Anesthesia and Analgesia
https://www.readbyqxmd.com/read/29239749/robotic-ureteral-reconstruction-using-buccal-mucosa-grafts-a-multi-institutional-experience
#8
Lee C Zhao, Aaron C Weinberg, Ziho Lee, Mark J Ferretti, Harry P Koo, Michael J Metro, Daniel D Eun, Michael D Stifelman
BACKGROUND: Minimally invasive treatment of long, multifocal ureteral strictures or failed pyeloplasty is challenging. Robot-assisted buccal mucosa graft ureteroplasty (RBU) is a technique for ureteral reconstruction that avoids the morbidity of bowel interposition or autotransplantation. OBJECTIVE: To evaluate outcomes for RBU in a multi-institutional cohort of patients treated for revision ureteropelvic junction obstruction and long or multifocal ureteral stricture at three tertiary referral centers...
November 24, 2017: European Urology
https://www.readbyqxmd.com/read/29239276/radiolabeled-anti-adenosine-triphosphate-synthase-monoclonal-antibody-as-a-theragnostic-agent-targeting-angiogenesis
#9
Bok-Nam Park, Su Jin Lee, Jung Hyun Roh, Kyung-Han Lee, Young-Sil An, Joon-Kee Yoon
INTRODUCTION: The potential of a radioiodine-labeled, anti-adenosine triphosphate synthase monoclonal antibody (ATPS mAb) as a theragnostic agent for simultaneous cancer imaging and treatment was evaluated. METHODS: Adenosine triphosphate synthase monoclonal antibody was labeled with radioiodine, then radiotracer uptake was measured in 6 different cancer cell lines. In vivo biodistribution was evaluated 24 and 48 hours after intravenous injection of 125I-ATPS mAb into MKN-45 tumor-bearing mice (n = 3)...
January 2017: Molecular Imaging
https://www.readbyqxmd.com/read/29238996/quality-management-of-a-massive-transfusion-protocol
#10
John R Hess, Patrick J Ramos, Nina E Sen, Virginia G Cruz-Cody, Erin E Tuott, Max J Louzon, Eileen M Bulger, Saman Arbabi, Monica B Pagano, Ryan A Metcalf
BACKGROUND: Massive transfusion is a response to massive uncontrolled hemorrhage. To be effective, it must be timely and address the patient's needs for blood volume, oxygen transport, and hemostasis. STUDY DESIGN AND METHODS: A review was performed on all activations of the massive transfusion protocol (MTP) in a hospital with large emergency medicine, trauma, and vascular surgery programs. Indications, transfused amounts, and outcomes were determined for each MTP event to determine appropriateness of MTP use...
December 13, 2017: Transfusion
https://www.readbyqxmd.com/read/29238962/population-pharmacokinetics-of-polymyxin-b
#11
Pooja Manchandani, Visanu Thamlikitkul, Yanina Dubrovskaya, Jessica T Babic, David C Lye, Lawrence S Lee, Vincent H Tam
Polymyxin B is used as a last treatment resort for multidrug-resistant Gram-negative bacterial infections. The objectives of this study were to examine the population pharmacokinetics of polymyxin B and investigate factor(s) influencing pharmacokinetic variability. Four serial blood samples each were collected from 35 adult patients at steady state. The concentrations of individual polymyxin B components were analyzed using a validated LCMS/MS assay and combined to derive total concentrations. A maximum likelihood expectation maximization approach was used to fit the data...
December 14, 2017: Clinical Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/29238690/tonsillectomy-comparative-study-of-various-techniques-and-changing-trend
#12
Ravinder Verma, Ravneet Ravinder Verma, Rohan Ravinder Verma
Tonsillectomy is a major surgical procedure in terms of volume in the general otolaryngological practice. It is a 3000-year-old surgical operation, referred in Hindu medicine. There has been a conceptual change in the indications and surgical technique in the last 40 years. A comparative study between the various methods of tonsillectomy was done. The study was carried out in the single institutional set up by the same surgeon but using different techniques. The study aimed at comparing the intra-operative factors (blood loss, time taken for surgery), postoperative results (pain, bleeding, dehydration, time taken for complete healing), and other complications like vomiting and hospitalization time between different groups of surgical methods...
December 2017: Indian Journal of Otolaryngology and Head and Neck Surgery
https://www.readbyqxmd.com/read/29238569/transient-cardiac-dysfunction-but-elevated-cardiac-and-kidney-biomarkers-24%C3%A2-h-following-an-ultra-distance-running-event-in-mexican-tarahumara
#13
Dirk L Christensen, Diana Espino, Rocío Infante-Ramírez, Mónica S Cervantes-Borunda, Rosa P Hernández-Torres, Antonio E Rivera-Cisneros, Daniel Castillo, Kate Westgate, Dijana Terzic, Soren Brage, Christian Hassager, Jens P Goetze, Jesper Kjaergaard
Background: The Mexican Tarahumara are accustomed to running ultra-distance races. No data exist on the acute physiological changes following ultra-distance running and physiological-biomarker associations in this population. Thus, we aimed to investigate the acute impact (≤ 24 h) on functional and biochemical changes of the cardiac muscle and biochemical changes associated with kidney function following a 63-km ultra-distance race with an altitude difference of 1800 m in Mexican Tarahumara athletes...
2017: Extreme Physiology & Medicine
https://www.readbyqxmd.com/read/29238514/diagnostic-value-of-glycemic-markers-hba1c-1-5-anhydroglucitol-and-glycated-albumin-in-evaluating-gestational-diabetes-mellitus
#14
Baris Saglam, Sezer Uysal, Sadik Sozdinler, Omer Erbil Dogan, Banu Onvural
Background: The oral glucose tolerance test (OGTT) is the current established method performed worldwide to diagnose gestational diabetes mellitus (GDM). The purpose of this study was to assess the utility of the use of long- and short-term markers of glycemic status. Methods: The study group was composed of 80 pregnant women, 40 with GDM and 40 with normal glucose tolerance. GDM was diagnosed with the American Diabetes Association criteria. Glycemic markers were measured in the OGTT blood samples of women at 24-28 weeks of gestation...
December 2017: Therapeutic Advances in Endocrinology and Metabolism
https://www.readbyqxmd.com/read/29237656/foot-strike-haemolysis-in-an-ultramarathon-runner
#15
Abid A Fazal, Mary S Whittemore, Katharine C DeGeorge
This case report describes mild anaemia and intravascular haemolysis in an otherwise healthy 41-year-old ultramarathon runner. In long-distance endurance athletes, trace gastrointestinal bleeding and plasma volume expansion are recognised sources of mild anaemia, often found incidentally. However, repetitive forceful foot striking can lead to blood cell lysis in the feet, resulting in a mild macrocytic anaemia and intravascular haemolysis, as was demonstrated in the patient described herein. Mild anaemia in runners, often called 'runner's pseudoanaemia', is typically clinically insignificant and does not require intervention...
December 13, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/29237474/dimethylarginines-in-patients-with-intracerebral-hemorrhage-association-with-outcome-hematoma-enlargement-and-edema
#16
Hans Worthmann, Na Li, Jens Martens-Lobenhoffer, Meike Dirks, Ramona Schuppner, Ralf Lichtinghagen, Jan T Kielstein, Peter Raab, Heinrich Lanfermann, Stefanie M Bode-Böger, Karin Weissenborn
BACKGROUND: Asymmetric dimethylarginine (ADMA)--the most potent endogenous NO-synthase inhibitor, has been regarded as mediator of endothelial dysfunction and oxidative stress. Considering experimental data, levels of ADMA and its structural isomer symmetric dimethylarginine (SDMA) might be elevated after intracerebral hemorrhage (ICH) and associated with clinical outcome and secondary brain injury. METHODS: Blood samples from 20 patients with acute ICH were taken at ≤ 24 h and 3 and 7 days after the event...
December 13, 2017: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/29235933/faster-ticking-rate-of-the-epigenetic-clock-is-associated-with-faster-pubertal-development-in-girls
#17
Alexandra M Binder, Camila Corvalan, Verónica Mericq, Ana Pereira, José Luis Santos, Steve Horvath, John Shepherd, Karin B Michels
Epigenetic age is an indicator of biological aging, capturing the impact of environmental and behavioral influences across time on cellular function. Deviance between epigenetic age and chronological age (AgeAccel) is a predictor of health. Pubertal timing has similarly been associated with cancer risk and mortality rate among females. We examined the association between AgeAccel and pubertal timing and adolescent breast composition in the longitudinal Growth and Obesity Cohort Study. AgeAccel was estimated in whole blood using the Horvath method at breast Tanner 2 (B2) and 4 (B4)...
December 13, 2017: Epigenetics: Official Journal of the DNA Methylation Society
https://www.readbyqxmd.com/read/29235099/variations-from-morning-to-afternoon-of-middle-cerebral-and-umbilical-artery-blood-flow-and-fetal-heart-rate-variability-and-fetal-characteristics-in-the-normally-developing-fetus
#18
Tehila Avitan, Ari Sanders, Ursula Brain, Dan Rurak, Tim F Oberlander, Ken Lim
OBJECTIVE: To determine if there are changes in maternal uterine blood flow, fetal brain blood flow, fetal heart rate variability, and umbilical blood flow between morning (AM) and afternoon (PM) in healthy, uncomplicated pregnancies. STUDY DESIGN: In this prospective study, 68 uncomplicated singleton pregnancies (mean 35 + 0.7 weeks gestation) underwent a standard observational protocol at both 08:00 (AM) and 13:30 (PM) of the same day. This protocol included Doppler measurements of uterine, umbilical, and fetal middle cerebral artery (MCA) volume flow parameters (flow, HR, peak systolic velocity [PSV], PI, and RI) followed by computerized cardiotocography...
December 13, 2017: Journal of Clinical Ultrasound: JCU
https://www.readbyqxmd.com/read/29235048/serum-lipid-enzyme-and-hematological-responses-of-broilers-fed-acacia-angustissima-leaf-meal-based-diets
#19
Sharai Ncube, Petronella Tapiwa Saidi, Musavengana Tapera Tivapasi, Venancio Edward Imbayarwo-Chikosi, Tinyiko Edward Halimani
The objective of the study was to determine serum lipid, enzyme, and hematological responses of broilers to Acacia angustissima leaf meal. Acacia angustissima leaves were harvested at mid maturity, ground into a leaf meal, and included in broiler diets at 0, 5, and 10%. One hundred and fifty-day-old chicks of 41.7 ± 1.560 g were randomly allocated to 15 groups of the three diets. Feed and water were provided ad libitum throughout the trial. At days 14, 28, and 42, 4 ml of blood were collected from the jugular vein in plain vacutainer tubes for alanine aminotransferase (ALT), alkaline phosphatase (ALP), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), and total cholesterol (TC) analyses...
December 12, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/29234869/a-controlled-trial-of-inhaled-bronchodilators-in-familial-dysautonomia
#20
Bat-El Bar-Aluma, Ori Efrati, Horacio Kaufmann, Jose-Alberto Palma, Lucy Norcliffe-Kaufmann
BACKGROUND: Chronic lung disease is a leading cause of premature death in patients with familial dysautonomia (FD). A significant number of patients have obstructive airway disease, yet it is not known whether this is pharmacologically reversible. METHODS: We conducted a double-blind, placebo-controlled, randomized clinical trial comparing the beta 2 agonist albuterol with the muscarinic blocker ipratropium bromide in patients homozygous for the IKBKAP founder mutation...
December 12, 2017: Lung
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