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https://www.readbyqxmd.com/read/28320530/single-cell-rna-sequence-analysis-of-mouse-glomerular-mesangial-cells-uncovers-mesangial-cell-essential-genes
#1
Yuqiu Lu, Yuting Ye, Qianqian Yang, Shaolin Shi
Mesangial cells are essential for the structure and function of glomeruli, but the mechanisms underlying these roles are not well understood. Here, we performed a single-cell RNA-sequence (RNA-seq) analysis of mouse mesangial cells using the Fluidigm C1 platform. We found that gene expression in individual mesangial cells was tremendously heterogeneous, with mean correlation coefficients of 0.20, and most mesangial genes were actually expressed in only a portion of mesangial cells and are therefore presumably dispensable...
March 17, 2017: Kidney International
https://www.readbyqxmd.com/read/28291821/transcriptomic-profile-of-leg-muscle-during-early-growth-in-chicken
#2
Qian Xue, Genxi Zhang, Tingting Li, Jiaojiao Ling, Xiangqian Zhang, Jinyu Wang
The early growth pattern, especially the age of peak growth, of broilers affects the time to market and slaughter weight, which in turn affect the profitability of the poultry industry. However, the underlying mechanisms regulating chicken growth and development have rarely been studied. This study aimed to identify candidate genes involved in chicken growth and investigated the potential regulatory mechanisms of early growth in chicken. RNA sequencing was applied to compare the transcriptomes of chicken muscle tissues at three developmental stages during early growth...
2017: PloS One
https://www.readbyqxmd.com/read/28284700/effects-of-dietary-neutral-detergent-fiber-and-starch-ratio-on-rumen-epithelial-cell-morphological-structure-and-gene-expression-in-dairy-cows
#3
L Ma, M Zhao, L S Zhao, J C Xu, J J Loor, D P Bu
This study was designed to investigate the effect of dietary neutral detergent fiber to starch ratio on rumen epithelial morphological structure and gene expression. Eight primiparous dairy cows including 4 ruminally fistulated cows were assigned to 4 total mixed rations with neutral detergent fiber to starch ratios of 0.86, 1.18, 1.63, and 2.34 in a replicated 4 × 4 Latin square design. The duration of each period was 21 d including 14 d for adaptation and 7 d for sampling. Rumen epithelial papillae were collected from the ruminally fistulated cows for morphological structure examination and mRNA expression analysis using quantitative real-time PCR of several genes related to volatile fatty acid absorption and metabolism, and cellular growth...
March 8, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28193157/whole-genome-association-analysis-of-pork-meat-ph-revealed-three-significant-regions-and-several-potential-genes-in-finnish-yorkshire-pigs
#4
Lucas L Verardo, Marja-Liisa Sevón-Aimonen, Timo Serenius, Ville Hietakangas, Pekka Uimari
BACKGROUND: One of the most commonly used quality measurements of pork is pH measured 24 h after slaughter. The most probable mode of inheritance for this trait is oligogenic with several known major genes, such as PRKAG3. In this study, we used whole-genome SNP genotypes of over 700 AI boars; after a quality check, 42,385 SNPs remained for association analysis. All the boars were purebred Finnish Yorkshire. To account for relatedness of the animals, a pedigree-based relationship matrix was used in a mixed linear model to test the effect of SNPs on pH measured from loin...
February 13, 2017: BMC Genetics
https://www.readbyqxmd.com/read/28157714/the-role-of-mir-24-as-a-race-related-genetic-factor-in-prostate-cancer
#5
Yutaka Hashimoto, Marisa Shiina, Taku Kato, Soichiro Yamamura, Yuichiro Tanaka, Shahana Majid, Sharanjot Saini, Varahram Shahryari, Priyanka Kulkarni, Pritha Desgupta, Yozo Mitsui, Mitsuho Sumida, Guoren Deng, Laura Tabatabai, Deepak Kumar, Rajvir Dahiya
The incidence of prostate cancer (PCa) among African-Americans (AfA) is significantly higher than Caucasian-Americans (CaA) but the genetic basis for this disparity is not known. To address this problem, we analyzed miRNA expression in AfA (n = 81) and CaA (n = 51) PCa patients. Here, we found that miR-24 is differentially expressed in AfA and CaA PCa patients and attempt to clarify its role in AfA patients. Also, the public sequencing data of the miR-24 promoter confirmed that it was highly methylated and down-regulated in PCa patients...
February 2, 2017: Oncotarget
https://www.readbyqxmd.com/read/28049695/dysregulated-dna-methyltransferase-3a-upregulates-igfbp5-to-suppress-trophoblast-cell-migration-and-invasion-in-preeclampsia
#6
Yuanhui Jia, Ting Li, Xiaojie Huang, Xianghong Xu, Xinyao Zhou, Linyan Jia, Jingping Zhu, Dandan Xie, Kai Wang, Qian Zhou, Liping Jin, Jiqin Zhang, Tao Duan
Preeclampsia is a unique multiple system disorder during human pregnancy, which affects ≈5% to 8% of pregnancies. Its risks and complications have become the major causes of maternal and fetal morbidity and mortality. Although abnormal placentation to which DNA methylation dysregulation is always linked is speculated to be one of the reasons causing preeclampsia, the underlying mechanisms still remain elusive to date. Here we revealed that aberrant DNA methyltransferase 3A (DNMT3A) plays a critical role in preeclampsia...
January 3, 2017: Hypertension
https://www.readbyqxmd.com/read/28008951/the-c-terminus-of-igfbp-5-suppresses-tumor-growth-by-inhibiting-angiogenesis
#7
Jae Ryoung Hwang, Young-Jae Cho, Yoonna Lee, Youngmee Park, Hee Dong Han, Hyung Jun Ahn, Je-Ho Lee, Jeong-Won Lee
Insulin-like growth factor-binding protein 5 (IGFBP-5) plays a role in cell growth, differentiation, and apoptosis. In this study, we found that IGFBP5 was markedly downregulated in ovarian cancer tissue. We investigated the functional significance of IGFBP-5 as a tumor suppressor. To determine functional regions of IGFBP-5, truncation mutants were prepared and were studied the effect on tumor growth. Expression of C-terminal region of IGFBP-5 significantly decreased tumor growth in an ovarian cancer xenograft...
December 23, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27957530/ipsilateral-and-contralateral-retinal-ganglion-cells-express-distinct-genes-during-decussation-at-the-optic-chiasm
#8
Qing Wang, Florencia Marcucci, Isadora Cerullo, Carol Mason
The increasing availability of transcriptomic technologies within the last decade has facilitated high-throughput identification of gene expression differences that define distinct cell types as well as the molecular pathways that drive their specification. The retinal projection neurons, retinal ganglion cells (RGCs), can be categorized into distinct morphological and functional subtypes and by the laterality of their projections. Here, we present a method for purifying the sparse population of ipsilaterally projecting RGCs in mouse retina from their contralaterally projecting counterparts during embryonic development through rapid retrograde labeling followed by fluorescence-activated cell sorting...
November 2016: ENeuro
https://www.readbyqxmd.com/read/27913274/two-grass-carp-ctenopharyngodon-idella-insulin-like-growth-factor-binding-protein-5-genes-exhibit-different-yet-conserved-functions-in-development-and-growth
#9
Guo-Dong Zheng, Chun-Xue Zhou, Si-Tong Lin, Jie Chen, Xia-Yun Jiang, Shu-Ming Zou
Insulin-like growth factor binding-protein 5 (igfbp5), the most conserved member of the IGFBP family in vertebrates, plays a critical role in controlling cell survival, growth, differentiation, and apoptosis. Here, we characterized the expression patterns of igfbp5a and igfbp5b in grass carp (Ctenopharyngodon idella), which are retained in many fish species, likely from the teleost-specific whole-genome duplication. Both igfbp5a and igfbp5b encode 268- and 263-aa peptides, respectively, which share a sequence identity of 71%...
February 2017: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/27906093/microrna-140-5p-inhibits-colorectal-cancer-invasion-and-metastasis-by-targeting-adamts5-and-igfbp5
#10
Lihui Yu, Ying Lu, Xiaocui Han, Wenyue Zhao, Jiazhi Li, Jun Mao, Bo Wang, Jie Shen, Shujun Fan, Lu Wang, Mei Wang, Lianhong Li, Jianwu Tang, Bo Song
BACKGROUND: Colorectal cancer (CRC) is one of the most common malignancies in the world. microRNA-140-5p (miR-140) has been shown to be involved in cartilage development and osteoarthritis (OA) pathogenesis. Some contradictions still exist concerning the role of miR-140 in tumor progression and metastasis, and the underlying mechanism is uncertain. METHODS: Immunohistochemistry was performed to determine the expressions of ADAMTS5 and IGFBP5 in CRC tissues. Human CRC cell lines HCT116 and RKO were transfected with miR-140 mimic, inhibitor, or small interfering RNA (siRNA) against ADAMTS5 or IGFBP5, respectively, using oligofectamine or lipofectamine 2000...
December 1, 2016: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/27893412/molecular-mechanism-and-therapeutic-implications-of-selinexor-kpt-330-in-liposarcoma
#11
Manoj Garg, Deepika Kanojia, Anand Mayakonda, Jonathan W Said, Ngan B Doan, Wenwen Chien, Trivadi S Ganesan, Linda Shyue Huey Chuang, Nachiyappan Venkatachalam, Erkan Baloglu, Sharon Shacham, Michael Kauffman, H Phillip Koeffler
Exportin-1 mediates nuclear export of multiple tumor suppressor and growth regulatory proteins. Aberrant expression of exportin-1 is noted in human malignancies, resulting in cytoplasmic mislocalization of its target proteins. We investigated the efficacy of selinexor against liposarcoma cells both in vitro and in vivo. Exportin-1 was highly expressed in liposarcoma samples and cell lines as determined by immunohistochemistry, western blot, and immunofluorescence assay. Knockdown of endogenous exportin-1 inhibited proliferation of liposarcoma cells...
January 31, 2017: Oncotarget
https://www.readbyqxmd.com/read/27835577/a-functional-variant-rs4442975-modulating-foxa1-binding-affinity-does-not-influence-the-risk-or-progression-of-breast-cancer-in-chinese-han-population
#12
Yi Zhang, Yan Li, Shaojie Jiang, Wei Chen, Jiao Lou, Juntao Ke, Jiaoyuan Li, Ying Zhu, Yajie Gong, Yang Yang, Jianbo Tian, Xiating Peng, Danyi Zou, Jing Gong, Jiang Chang, Xiaoping Miao, Rong Zhong
The DNA-binding protein FOXA1 has been shown to regulate nearly all estrogen receptor-chromatin interactions, thereby influencing target gene expression levels in breast cancer (BC) cells. Recently, the rs4442975 T-allele, which disrupts the recruitment of FOXA1 and interacts with the IGFBP5 promoter, was associated to BC susceptibility in a European population. We conducted a hospital-based case-control study that included 1227 cases and 1285 controls to explore the potential association between rs4442975 and BC risk in Chinese Han population, and the effect of this SNP on BC progression was also observed in cases...
November 7, 2016: Oncotarget
https://www.readbyqxmd.com/read/27800570/mir-143-regulates-proliferation-and-differentiation-of-bovine-skeletal-muscle-satellite-cells-by-targeting-igfbp5
#13
Wei Ran Zhang, Hui Na Zhang, Yi Min Wang, Yang Dai, Xin Feng Liu, Xin Li, Xiang Bin Ding, Hong Guo
Development of skeletal muscle is a complicated biological process regulated by various regulation factors and signal pathways. MicroRNAs (miRNAs) are novel gene regulators that control muscle cell development. microRNA-143 (miR-143) is highly expressed in skeletal muscle, and we found that miR-143 level is significantly increased during bovine skeletal muscle satellite cells (MSCs) differentiation process through microarray analysis and qRT-PCR detection. However, the function of miR-143 in bovine muscle development remained unclear...
March 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/27706699/increased-mir-155-5p-expression-in-dermal-mesenchymal-stem-cells-of-psoriatic-patients-comparing-the-microrna-expression-profile-by-microarray
#14
R X Hou, R F Liu, X C Zhao, Y R Jia, P An, Z P Hao, J Q Li, X H Li, G H Yin, K M Zhang
Mesenchymal stem cells (MSCs) have pleiotropic immuno-modulatory effects and pro-angiogenic ability, leading to the presumption that MSCs may be involved in the pathogenesis of many inflammatory or autoimmune disorders, including psoriasis. In a previous study, we reported the specific gene expression profile of dermal MSCs from psoriasis. Inflammation- and angiogenesis-related genes, such as lipopolysaccharide-induced tumor necrosis factor-alpha transcription factor (LITAF), dual-specificity protein phosphatase 1 (DUSP1), vascular endothelial growth factor α (VEGFα), and insulin-like growth factor-binding protein-5 (IGFBP5), are abnormally expressed in psoriatic dermal MSCs...
September 2, 2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/27664381/moderate-high-or-low-maternal-protein-diets-change-gene-expression-but-not-the-phenotype-of-skeletal-muscle-from-porcine-fetuses
#15
C Kalbe, D Lösel, J Block, L Lefaucheur, K-P Brüssow, O Bellmann, R Pfuhl, B Puppe, W Otten, C C Metges, C Rehfeldt
The aim of our study was to characterize the immediate phenotypic and adaptive regulatory responses of fetuses to different in utero conditions reflecting inadequate maternal protein supply during gestation. The gilts fed high- (250% above control) or low- (50% under control) protein diets isoenergetically adjusted at the expense of carbohydrates from the day of insemination until the fetuses were collected at day 64 or 94 of gestation. We analyzed body composition, histomorphology, biochemistry, and messenger RNA (mRNA) expression of fetal skeletal muscle...
January 2017: Domestic Animal Endocrinology
https://www.readbyqxmd.com/read/27639961/marsupial-tammar-wallaby-delivers-milk-bioactives-to-altricial-pouch-young-to-support-lung-development
#16
Vengamanaidu Modepalli, Lyn A Hinds, Julie A Sharp, Christophe Lefevre, Kevin R Nicholas
Our research is exploiting the marsupial as a model to understand the signals required for lung development. Marsupials have a unique reproductive strategy, the mother gives birth to altricial neonate with an immature lung and the changes in milk composition during lactation in marsupials appears to provide bioactives that can regulate diverse aspects of lung development, including branching morphogenesis, cell proliferation and cell differentiation. These effects are seen with milk collected between 25 and 100days postpartum...
November 2016: Mechanisms of Development
https://www.readbyqxmd.com/read/27619487/transcriptional-regulation-of-genes-involved-in-retinoic-acid-metabolism-in-senegalese-sole-larvae
#17
Anaïs Boglino, Marian Ponce, Xavier Cousin, Enric Gisbert, Manuel Manchado
The aim of this study was the characterization of transcriptional regulatory pathways mediated by retinoic acid (RA) in Senegalese sole larvae. For this purpose, pre-metamorphic larvae were treated with a low concentration of DEAB, an inhibitor of RALDH enzyme, until the end of metamorphosis. No differences in growth, eye migration or survival were observed. Nevertheless, gene expression analysis revealed a total of 20 transcripts differentially expressed during larval development and only six related with DEAB treatments directly involved in RA metabolism and actions (rdh10a, aldh1a2, crbp1, igf2r, rarg and cyp26a1) to adapt to a low-RA environment...
January 2017: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/27612043/correlation-between-the-dna-methylation-and-gene-expression-of-igfbp5-in-breast%C3%A2-cancer
#18
Sevgi Karabulut, Zehra Kaya, Gökçe Gūllū Amuran, Irem Peker, Tolga Özmen, Bahadır M Gūllūoḡlu, Handan Kaya, Can Erzik, Ayşe Ōzer, Mustafa Akkiprik
BACKGROUND: The insulin-like growth factor binding protein5 (IGFBP5) is often dysregulated in human cancers and considered neither a tumor suppressor nor an oncogene. OBJECTIVE: We aim to examine the reason of the changeable gene regulation of IGFBP5 in the case of methylation in breast cancer. METHODS: We used methyl-specific polymerase (MSP) chain reaction to detect CpG methylation of IGFBP5 promoter and exon-I in breast cancer and adjacent tissues...
2016: Breast Disease
https://www.readbyqxmd.com/read/27610142/characterization-of-cellular-and-molecular-heterogeneity-of-bone-marrow-stromal-cells
#19
Mona Elsafadi, Muthurangan Manikandan, Muhammad Atteya, Jamil Amjad Hashmi, Zafar Iqbal, Abdullah Aldahmash, Musaad Alfayez, Moustapha Kassem, Amer Mahmood
Human bone marrow-derived stromal stem cells (hBMSC) exhibit multiple functions, including differentiation into skeletal cells (progenitor function), hematopoiesis support, and immune regulation (nonprogenitor function). We have previously demonstrated the presence of morphological and functional heterogeneity of hBMSC cultures. In the present study, we characterized in detail two hTERT-BMSC clonal cell populations termed here CL1 and CL2 that represent an opposing phenotype with respect to morphology, markers expression: alkaline phosphatase (ALP) and CD146, and ex vivo differentiation potential...
2016: Stem Cells International
https://www.readbyqxmd.com/read/27547236/2-amino-1-methyl-6-phenylimidazo-4-5-b-pyridine-phip-induces-gene-expression-changes-in-jak-stat-and-mapk-pathways-related-to-inflammation-diabetes-and-cancer
#20
Lora J Rogers, Alexei G Basnakian, Mohammed S Orloff, Baitang Ning, Aiwei Yao-Borengasser, Vinay Raj, Susan Kadlubar
BACKGROUND: 2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine (PhIP), a heterocyclic aromatic amine (HCA) formed in meat that is cooked at high temperatures and then ingested, can potentially be retained in human adipose tissues. METHODS: To determine if PhIP is bioactive in the adipocyte, we exposed a human adipocyte cell line,HepG2 and Caco-2 cells to low dose PhIP. Uptake and retention of PhIP was determined and cytotoxicity was assessed by the TUNEL assay. Relative expression of PhIP-activating genes (CYP1A1, CYP1A2, SULT1A1 and UGT1A1) was determined by RT-PCR and global expression changes were also examined...
2016: Nutrition & Metabolism
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