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Bone marrow derived stem cells

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https://www.readbyqxmd.com/read/29236047/hasc-and-dfat-multipotent-stem-cells-for-regenerative-medicine-a-comparison-of-their-potential-differentiation-in-vitro
#1
Marco Saler, Laura Caliogna, Laura Botta, Francesco Benazzo, Federica Riva, Giulia Gastaldi
Adipose tissue comprises both adipose and non-adipose cells such as mesenchymal stem cells. These cells show a surface antigenic profile similar to that of bone-marrow-derived MSC. The cells derived from the dedifferentiation of mature adipocytes (DFAT) are another cell population with characteristics of stemness. The aim of this study is to provide evidence of the stemness, proliferation, and differentiation of human adipose stem cells (hASC) and DFAT obtained from human subcutaneous AT and evaluate their potential use in regenerative medicine...
December 13, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29235254/mesenchymal-stem-cells-a-double-edged-sword-in-radiation-induced-lung-injury
#2
Yi Yao, Zhongliang Zheng, Qibin Song
Radiation therapy is an important treatment modality for multiple thoracic malignancies. However, radiation-induced lung injury (RILI), which is the term generally used to describe damage to the lungs caused by exposure to ionizing radiation, remains a critical issue affecting both tumor control and patient quality of life. Despite tremendous effort, there is no current consensus regarding the optimal treatment approach for RILI. Because of a number of functional advantages, including self-proliferation, multi-differentiation, injury foci chemotaxis, anti-inflammation, and immunomodulation, mesenchymal stem cells (MSCs) have been a focus of research for many years...
December 13, 2017: Thoracic Cancer
https://www.readbyqxmd.com/read/29231967/therapeutic-potential-of-porcine-bronchoalveolar-fluid-derived-mesenchymal-stromal-cells-in-a-pig-model-of-lps-induced-ali
#3
Mahesh Khatri, Levi Arthur Richardson
In this study, we isolated mesenchymal stromal (stem) cells (MSCs) from broncho-alveolar lavage fluid (BAL) of 2-6 week-old commercial pigs. BAL-MSCs displayed fibroblastic morphology and possessed self-renewal properties. Similar to bone-marrow MSCs, BAL-MSCs expressed mesenchymal markers and both cell types lacked the expression of hematopoetic markers. BAL-MSCs, when cultured in differentiation induction media, differentiated into adipocytes, osteocytes and chondrocytes. Next, we examined if BAL-MSCs have the ability to treat lipopolysaccharide (LPS)-induced acute lung injury (ALI) in a pig model...
December 12, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29230389/cyclic-tensile-strain-can-play-a-role-in-directing-both-intramembranous-and-endochondral-ossification-of-mesenchymal-stem-cells
#4
Simon F Carroll, Conor T Buckley, Daniel J Kelly
Successfully regenerating damaged or diseased bone and other joint tissues will require a detailed understanding of how joint specific environmental cues regulate the fate of progenitor cells that are recruited or delivered to the site of injury. The goal of this study was to explore the role of cyclic tensile strain (CTS) in regulating the initiation of mesenchymal stem cell/multipotent stromal cell (MSC) differentiation, and specifically their progression along the endochondral pathway. To this end, we first explored the influence of CTS on the differentiation of MSCs in the absence of any specific growth factor, and secondly, we examined the influence of the long-term application of this mechanical stimulus on markers of endochondral ossification in MSCs maintained in chondrogenic culture conditions...
2017: Frontiers in Bioengineering and Biotechnology
https://www.readbyqxmd.com/read/29230181/calvarial-suture-derived-stem-cells-and-their-contribution-to-cranial-bone-repair
#5
REVIEW
Daniel H Doro, Agamemnon E Grigoriadis, Karen J Liu
In addition to the natural turnover during life, the bones in the skeleton possess the ability to self-repair in response to injury or disease-related bone loss. Based on studies of bone defect models, both processes are largely supported by resident stem cells. In the long bones, the source of skeletal stem cells has been widely investigated over the years, where the major stem cell population is thought to reside in the perivascular niche of the bone marrow. In contrast, we have very limited knowledge about the stem cells contributing to the repair of calvarial bones...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/29228730/wisp2-disruption-represses-cxcr4-expression-and-inhibits-bmscs-homing-to-injured-liver
#6
Dan Qin, Yi Yan, Bian Hu, Wanpo Zhang, Hanmin Li, Xiaodong Li, Shenghui Liu, Depeng Dai, Xiongji Hu, Xingxu Huang, Lisheng Zhang
Liver regeneration/repair is a compensatory regrowth following acute liver failure, and bone marrow-derived mesenchyme stem cell (BMSC) transplantation is an effective therapy that promotes liver regeneration/repair. Wnt1 inducible signaling pathway protein 2 (Wisp2) is highly expressed in BMSCs, however, its function remains unclear. In this work, we used clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein -9 nuclease (CRISPR/Cas9) genome editing technology to knockdown Wisp2 in BMSCs, and these modified cells were then transplanted into rats which were induced by the 2-AAF/PH...
November 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29228693/bone-marrow-stem-cells-derived-extracellular-matrix-is-a-promising-material
#7
Xiaoyan Wang, Guanghua Chen, Chao Huang, Hualei Tu, Jilong Zou, Jinglong Yan
The extracellular matrix(ECM), which is primarily composed of collagens and proteoglycans, plays a key role in cell proliferation, differentiation, and migration and interactions between cells. In this study, we produced chitosan/gelatin/bone marrow stem cells-derived extracellular matrix(C/G/BMSCs-dECM) scaffolds via lyophilization and cross-linking, and chitosan/gelatin(C/G) scaffolds were used as controls. For the C/G/BMSCs-dECM scaffolds, the average pore size was 289.17 ± 80.28 μm; the average porosity was 89...
November 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29227527/m2-muscarinic-receptor-activation-inhibits-cell-proliferation-and-migration-of-rat-adipose-mesenchymal-stem-cells
#8
Roberta Piovesana, Simona Melfi, Mario Fiore, Valerio Yi, Ada Maria Tata
Mesenchymal stem cells (MSCs), also known as stromal mesenchymal stem cells, are multipotent cells, which can be found in many tissues and organs as bone marrow, adipose tissue and other tissues. In particular MSCs derived from Adipose tissue (ADSCs) are the most frequently used in regenerative medicine because they are easy to source, rapidly expandable in culture and excellent differentiation potential into adipocytes, chondrocytes and other cell types. Acetylcholine (ACh), is one of the most important neurotransmitter in central (CNS) and peripheral nervous system (PNS), playing important roles also in non-neural tissue, but its functions in MSCs are still not investigated...
December 11, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29226477/cell-therapy-for-critical-limb-ischemia-an-integrated-review-of-pre-clinical-and-clinical-studies
#9
REVIEW
Mohammad Qadura, Daniella C Terenzi, Subodh Verma, Mohammed Al-Omran, David A Hess
Critical limb ischemia (CLI), the most severe form of peripheral artery disease, is characterized by pain at rest and non-healing ulcers in the lower extremities. For patients with CLI, where the extent of atherosclerotic artery occlusion is too severe for surgical bypass or percutaneous interventions, limb amputation remains the only treatment option. Thus, cell-based therapy to restore perfusion and promote wound healing in patients with CLI is under intense investigation. Despite promising preclinical studies in animal models, transplantation of bone marrow (BM) derived cell populations in patients with CLI has shown limited benefit preventing limb amputation...
December 11, 2017: Stem Cells
https://www.readbyqxmd.com/read/29225627/isolation-and-molecular-characterization-of-amniotic-fluid-derived-mesenchymal-stem-cells-obtained-from-caesarean-sections
#10
Lucas-Sebastian Spitzhorn, Md Shaifur Rahman, Laura Schwindt, Huyen-Tran Ho, Wasco Wruck, Martina Bohndorf, Silke Wehrmeyer, Audrey Ncube, Ines Beyer, Carsten Hagenbeck, Percy Balan, Tanja Fehm, James Adjaye
Human amniotic fluid cells are immune-privileged with low immunogenicity and anti-inflammatory properties. They are able to self-renew, are highly proliferative, and have a broad differentiation potential, making them amenable for cell-based therapies. Amniotic fluid (AF) is routinely obtained via amniocentesis and contains heterogeneous populations of foetal-derived progenitor cells including mesenchymal stem cells (MSCs). In this study, we isolated human MSCs from AF (AF-MSCs) obtained during Caesarean sections (C-sections) and characterized them...
2017: Stem Cells International
https://www.readbyqxmd.com/read/29225483/active-stromal-cell-derived-factor-1%C3%AE-and-endothelial-progenitor-cells-are-equally-increased-by-alogliptin-in-good-and-poor-diabetes-control
#11
Roberto Negro, Eupremio Luigi Greco, Giacomo Greco
Background: It is postulated that the ability of dipeptidyl peptidase-4 inhibitors (DPP-4-i) to increase circulating endothelial progenitor cells (EPCs) may be at least partly mediated by active stromal cell-derived factor 1α (SDF-1α) (a pivotal mediator of stem cell mobilization from the bone marrow). As other DPP-4-i were demonstrated to increase EPC concentrations, in this study, we sought to investigate the ability of the DPP-4-i alogliptin in modifying EPCs and SDF-1α, in patients with good and poor diabetes control...
2017: Clinical Medicine Insights. Endocrinology and Diabetes
https://www.readbyqxmd.com/read/29224961/effective-combination-of-methylprednisolone-and-interferon-%C3%AE-secreting-mesenchymal-stem-cells-in-a-model-of-multiple-sclerosis
#12
Mi Jin Kim, Jung Yeon Lim, Soon A Park, Sang In Park, Won Shik Kim, Chung Heon Ryu, Sin-Soo Jeun
Methylprednisolone (MP) has been recommended as a standard drug in MS therapies. We previously demonstrated that IFNβ-secreting human bone marrow-derived mesenchymal stem cells (MSCs-IFNβ) exert immunomodulatory effects in experimental autoimmune encephalomyelitic (EAE) mice. In this study, we evaluated whether a combined treatment of MP and MSCs-IFNβ had enhanced therapeutic effects on EAE mice. The combination treatment resulted in enhanced immunomodulatory effects, including reduced production of pro-inflammatory cytokines and increased production of anti-inflammatory cytokines...
November 29, 2017: Journal of Neuroimmunology
https://www.readbyqxmd.com/read/29224250/multiple-autologous-bone-marrow-derived-cd271-mesenchymal-stem-cell-transplantation-overcomes-drug-resistant-epilepsy-in-children
#13
Olga Milczarek, Danuta Jarocha, Anna Starowicz-Filip, Stanislaw Kwiatkowski, Bogna Badyra, Marcin Majka
There is a need among patients suffering from drug-resistant epilepsy (DRE) for more efficient and less toxic treatments. The objective of the present study was to assess the safety, feasibility, and potential efficacy of autologous bone marrow cell transplantation in pediatric patients with DRE. Two females and two males (11 months to 6 years) were enrolled and underwent a combined therapy consisting of autologous bone marrow nucleated cells (BMNCs) transplantation (intrathecal: 0.5 × 109 ; intravenous: 0...
December 10, 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/29222849/bone-marrow-transplantation-improves-motor-activity-in-a-mouse-model-of-ataxia
#14
David Díaz, Marina Piquer-Gil, Javier Sánchez Recio, María Magdalena Martínez-Losa, José Ramón Alonso, Eduardo Weruaga, Manuel Álvarez-Dolado
Ataxias are locomotor disorders that can have an origin both neural and muscular, although both impairments are related. Unfortunately, ataxia has no cure and the current therapies are aimed at motor re-education or muscular reinforcement. Nevertheless, cell therapy is becoming a promising approach to deal with incurable neural diseases, including neuro-muscular ataxias. Here we have used a model of ataxia, the PCD mutant mouse, to study the effect of healthy (wild-type) bone marrow transplantation on the restoration of defective mobility...
December 8, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29222293/current-treatment-algorithm-for-the-management-of-lower-risk-mds
#15
REVIEW
Aristoteles Giagounidis
Lower risk myelodysplastic syndromes (MDS), defined as MDS with a Revised International Prognostic Scoring System score ≤3.5 points, will remain a challenging entity in 2018. Supportive care continues to be the linchpin of treatment, although the options to reduce transfusion needs are broadening. To achieve red blood cell transfusion independence in non-del(5q) patients, erythropoiesis-stimulating agents remain a mainstay of therapy as long as endogenous erythropoietin levels are <500 U/L (and preferably <200 U/L)...
December 8, 2017: Hematology—the Education Program of the American Society of Hematology
https://www.readbyqxmd.com/read/29216265/oct4-expression-mediates-partial-cardiomyocyte-reprogramming-of-mesenchymal-stromal-cells
#16
Gustavo Yannarelli, Natalia Pacienza, Sonia Montanari, Diego Santa-Cruz, Sowmya Viswanathan, Armand Keating
Mesenchymal stem/stromal cells (MSCs) are in numerous cell therapy clinical trials, including for injured myocardium. Acquisition of cardiomyocyte characteristics by MSCs may improve cardiac regeneration but the mechanisms regulating this process are unclear. Here, we investigated whether the pluripotency transcription factor OCT4 is involved in the activation of cardiac lineage genetic programs in MSCs. We employed our established co-culture model of MSCs with rat embryonic cardiomyocytes showing co-expression of cardiac markers on MSCs independent of cell fusion...
2017: PloS One
https://www.readbyqxmd.com/read/29215736/protective-effect-of-epigenetic-silencing-of-cyclind1-against-spinal-cord-injury-using-bone-marrow-derived-mesenchymal-stem-cells-in-rats
#17
Yuan Wang, Qing-Jie Kong, Jin-Chuan Sun, Xi-Ming Xu, Yong Yang, Ning Liu, Jian-Gang Shi
OBJECTIVE: This study focuses on the protective effect of epigenetic silencing of CyclinD1 against spinal cord injury (SCI) using bone marrow-derived mesenchymal stem cells (BMSCs) in rats. METHODS: Eighty-eight adult female Wistar rats were randomly assigned into the sham group, the control group, the si-CyclinD1 + BMSCs group and the BMSCs group. CyclinD1 protein and mRNA expressions after siRNA transfection were detected by western blotting and qRT-PCR. The siRNA-CyclinD1 BMSCs were transplanted into rats in the si-CyclinD1 + BMSCs group using stereotaxic method 6 h after SCI...
December 7, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29214990/rna-sequencing-reveals-a-transcriptomic-portrait-of-human-mesenchymal-stem-cells-from-bone-marrow-adipose-tissue-and-palatine-tonsils
#18
Kyung-Ah Cho, Minhwa Park, Yu-Hee Kim, So-Youn Woo, Kyung-Ha Ryu
Human mesenchymal stem cells (MSCs) are adult multipotent cells that have plasticity and inhabit the stroma of diverse tissues. The potential utility of MSCs has been heavily investigated in the fields of regenerative medicine and cell therapy. However, MSCs represent diverse populations that may depend on the tissue of origin. Thus, the ability to identify specific MSC populations has remained difficult. Using RNA sequencing, we analyzed the whole transcriptomes of bone marrow-derived MSCs (BMs), adipose tissue-derived MSCs (AMs), and tonsil-derived MSCs (TMs)...
December 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29212377/gene-therapy-for-bone-repair-using-human-cells-superior-osteogenic-potential-of-bmp-2-transduced-mesenchymal-stem-cells-derived-from-adipose-tissue-compared-to-bone-marrow
#19
Sofia Bougioukli, Osamu Sugiyama, William Pannell, Brandon Ortega, Matthew Tan, Amy Tang, Robert Yoho, Daniel A Oakes, Jay R Lieberman
Ex vivo regional gene therapy strategies using animal mesenchymal stem cells genetically modified to overexpress osteoinductive growth factors has been successfully used in a variety of animal models to induce both heterotopic and orthotopic bone formation. However, in order to adapt regional gene therapy for clinical applications it is essential to assess the osteogenic capacity of transduced human cells and choose the cell type that demonstrates the best clinical potential. Bone marrow stem cells (BMSC) and adipose-derived stem cells (ASC) were selected in our study for in vitro evaluation, before and after transduction with a lentiviral two-step transcriptional amplification system (TSTA) overexpressing BMP-2 (LV-TSTA-BMP-2) or GFP (LV-TSTA-GFP); cell growth, transduction efficiency, BMP-2 production and osteogenic capacity were assessed...
December 6, 2017: Human Gene Therapy
https://www.readbyqxmd.com/read/29211118/venous-blood-derivatives-as-fbs-substitutes-for-mesenchymal-stem-cells-a-systematic-scoping-review
#20
Luiz A Chisini, Marcus C M Conde, Guillermo Grazioli, Alissa S San Martin, Rodrigo Varella de Carvalho, Jacques E Nör, Flávio F Demarco
Although the biological properties of mesenchymal stem cells (MSC) are well-characterized in vitro, MSC clinical application is still far away to be achieved, mainly due to the need of xenogeneic substances for cell expansion, such as fetal bovine serum (FBS). FBS presents risks regarding pathogens transmissions and internalization of animal's proteins, which can unleash antigenic responses in patients after MSC implantation. A wide range of venous blood derivatives (VBD) has been reported as FBS substitutes showing promising results...
November 2017: Brazilian Dental Journal
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