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Bone marrow derived stem cells

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https://www.readbyqxmd.com/read/27906056/umbilical-cord-blood-derived-non-hematopoietic-stem-cells-retrieved-and-expanded-on-bone-marrow-derived-extracellular-matrix-display-pluripotent-characteristics
#1
Junjie Wu, Yun Sun, Travis J Block, Milos Marinkovic, Zhi-Liang Zhang, Richard Chen, Yixia Yin, Juquan Song, David D Dean, Zhongding Lu, Xiao-Dong Chen
BACKGROUND: Umbilical cord blood (UCB) not only contains hematopoietic stem cells (HSCs), but also non-hematopoietic stem cells (NHSCs) that are able to differentiate into a number of distinct cell types. Based on studies published to date, the frequency of NHSCs in UCB is believed to be very low. However, the isolation of these cells is primarily based on their adhesion to tissue culture plastic surfaces. METHODS AND RESULTS: In the current study, we demonstrate that this approach overlooks some of the extremely immature NHSCs because they lack the ability to adhere to plastic...
December 1, 2016: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/27905403/infusion-with-human-bone-marrow-derived-mesenchymal-stem-cells-improves-%C3%AE-cell-function-in-patients-and-non-obese-mice-with-severe-diabetes
#2
Lirong Li, Hui Hui, Xiaolei Jia, Jie Zhang, Ying Liu, Qianyue Xu, Dalong Zhu
Mesenchymal stem cells (MSCs) transplantation is a promising therapeutic strategy for type 1 diabetes (T1D). However, little is known on whether MSC transplantation can benefit T1D patients with ketoacidosis and its potential actions. Here, we show that infusion with bone marrow MSCs preserves β-cell function in some T1D patients with ketoacidosis by decreasing exogenous insulin requirement and increasing plasma C-peptide levels up to 1-2 years. MSC transplantation increased plasma and islet insulin contents in non-obese diabetic (NOD) mice with severe diabetes...
December 1, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27904503/stem-cell-ophthalmology-treatment-study-scots-bone-marrow-derived-stem-cells-in-the-treatment-of-leber-s-hereditary-optic-neuropathy
#3
Jeffrey N Weiss, Steven Levy, Susan C Benes
The Stem Cell Ophthalmology Treatment Study (SCOTS) is currently the largest-scale stem cell ophthalmology trial registered at ClinicalTrials.gov (identifier: NCT01920867). SCOTS utilizes autologous bone marrow-derived stem cells (BMSCs) to treat optic nerve and retinal diseases. Treatment approaches include a combination of retrobulbar, subtenon, intravitreal, intra-optic nerve, subretinal, and intravenous injection of autologous BMSCs according to the nature of the disease, the degree of visual loss, and any risk factors related to the treatments...
October 2016: Neural Regeneration Research
https://www.readbyqxmd.com/read/27904036/differential-regulation-of-lympho-myelopoiesis-by-stromal-cells-in-the-early-and-late-phases-in-balb-c-mice-repeatedly-exposed-to-lipopolysaccharide
#4
Isao Tsuboi, Tomonori Harada, Yoko Hirabayashi, Jun Kanno, Shin Aizawa
Chronic lipopolysaccharide (LPS) exposure to mice reduces the lymphoid compartment and skews the hematopoietic cell compartment toward myeloid-cells, which is considered to be a direct effect of LPS on hematopoietic stem cells. However, the effect of chronic LPS exposure on stromal-cells, which compose the hematopoietic microenvironment, has not been elucidated. Here, we investigated early- and late-phase effects of repeated LPS exposure on stromal-cells. During the early phase, when mice were treated with 5 or 25 µg LPS three times at weekly intervals, the numbers of myeloid-progenitor (colony forming unit-granulocyte macrophage (CFU-GM)) cells and B lymphoid-progenitor (CFU-preB) cells in the bone-marrow (BM) rapidly decreased after each treatment...
2016: Biological & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/27899899/cell-expansion-dependent-inflammatory-and-metabolic-profile-of-human-bone-marrow-mesenchymal-stem-cells
#5
Patricia Prieto, María Fernández-Velasco, María E Fernández-Santos, Pedro L Sánchez, Verónica Terrón, Paloma Martín-Sanz, Francisco Fernández-Avilés, Lisardo Boscá
Stem cell therapy has emerged as a promising new area in regenerative medicine allowing the recovery of viable tissues. Among the many sources of adult stem cells, bone marrow-derived are easy to expand in culture via plastic adherence and their multipotentiality for differentiation make them ideal for clinical applications. Interestingly, several studies have indicated that MSCs expansion in vitro may be limited mainly due to "cell aging" related to the number of cell divisions in culture. We have determined that MSCs exhibit a progressive decline across successive passages in the expression of stem cell markers, in plasticity and in the inflammatory response, presenting low immunogenicity...
2016: Frontiers in Physiology
https://www.readbyqxmd.com/read/27899358/cytohesin-1-regulates-homing-and-engraftment-of-human-hematopoietic-stem-and-progenitor-cells
#6
Justyna Rak, Katie Foster, Katarzyna Potrzebowska, Mehrnaz Safaee Talkhoncheh, Natsumi Miharada, Karolina Komorowska, Therese Torngren, Anders Kvist, Åke Borg, Lena Svensson, Dominique Bonnet, Jonas Larsson
Adhesion is a key component of hematopoietic stem cell regulation mediating homing and retention to the niche in the bone marrow. Here, using an RNA interference screen, we identify cytohesin 1 (CYTH1) as a critical mediator of adhesive properties in primary human cord-blood derived hematopoietic stem and progenitor cells (HSPCs). Knockdown of CYTH1 disrupted adhesion of HSPC to primary human mesenchymal stroma cells (MSCs). Attachment to fibronectin and ICAM1, two integrin ligands, was severely impaired, and CYTH1 deficient cells showed a reduced integrin β1 activation response, suggesting that CYTH1 mediates integrin-dependent functions...
November 29, 2016: Blood
https://www.readbyqxmd.com/read/27898509/acute-achilles-tendon-ruptures-an-update-on-treatment
#7
Anish R Kadakia, Robert G Dekker, Bryant S Ho
Acute rupture of the Achilles tendon is common and seen most frequently in people who participate in recreational athletics into their thirties and forties. Although goals of treatment have not changed in the past 15 years, recent studies of nonsurgical management, specifically functional bracing with early range of motion, demonstrate rerupture rates similar to those of tendon repair and result in fewer wound and soft-tissue complications. Satisfactory outcomes may be obtained with nonsurgical or surgical treatment...
November 24, 2016: Journal of the American Academy of Orthopaedic Surgeons
https://www.readbyqxmd.com/read/27897419/exercise-restores-muscle-stem-cell-mobilization-regenerative-capacity-and-muscle-metabolic-alterations-via-adiponectin-adipor1-activation-in-samp10-mice
#8
Aiko Inoue, Xian Wu Cheng, Zhe Huang, Lina Hu, Ryosuke Kikuchi, Haiying Jiang, Limei Piao, Takeshi Sasaki, Kohji Itakura, Hongxian Wu, Guangxian Zhao, Yanna Lei, Guang Yang, Enbo Zhu, Xiang Li, Kohji Sato, Teruhiko Koike, Masafumi Kuzuya
BACKGROUND: Exercise train (ET) stimulates muscle response in pathological conditions, including aging. The molecular mechanisms by which exercise improves impaired adiponectin/adiponectin receptor 1 (AdipoR1)-related muscle actions associated with aging are poorly understood. Here we observed that in a senescence-accelerated mouse prone 10 (SAMP10) model, long-term ET modulated muscle-regenerative actions. METHODS: 25-week-old male SAMP10 mice were randomly assigned to the control and the ET (45 min/time, 3/week) groups for 4 months...
November 29, 2016: Journal of Cachexia, Sarcopenia and Muscle
https://www.readbyqxmd.com/read/27897136/an-image-based-skeletal-dosimetry-model-for-the-icrp-reference-adult-female-internal-electron-sources
#9
Shannon E O'Reilly, Lindsay S DeWeese, Matthew R Maynard, Didier A Rajon, Michael B Wayson, Emily L Marshall, Wesley E Bolch
An image-based skeletal dosimetry model for internal electron sources was created for the ICRP-defined reference adult female. Many previous skeletal dosimetry models, which are still employed in commonly used internal dosimetry software, do not properly account for electron escape from trabecular spongiosa, electron cross-fire from cortical bone, and the impact of marrow cellularity on active marrow self-irradiation. Furthermore, these existing models do not employ the current ICRP definition of a 50 µm bone endosteum (or shallow marrow)...
November 29, 2016: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/27895039/region-specific-effect-of-the-decellularized-meniscus-extracellular-matrix-on-mesenchymal-stem-cell-based-meniscus-tissue-engineering
#10
Kazunori Shimomura, Benjamin B Rothrauff, Rocky S Tuan
BACKGROUND: The meniscus is the most commonly injured knee structure, and surgical repair is often ineffective. Tissue engineering-based repair or regeneration may provide a needed solution. Decellularized, tissue-derived extracellular matrices (ECMs) have received attention for their potential use as tissue-engineered scaffolds. In considering meniscus-derived ECMs (mECMs) for meniscus tissue engineering, it is noteworthy that the inner and outer regions of the meniscus have different structural and biochemical features, potentially directing the differentiation of cells toward region-specific phenotypes...
November 28, 2016: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/27894122/b-cell-lymphoma-2-modified-bone-marrow-derived-mesenchymal-stem-cells-transplantation-for-the-treatment-of-diabetes-mellitus-induced-erectile-dysfunction-in-a-rat-model
#11
Xiang Sun, Long-Hua Luo, Liang Feng, Dong-Shui Li, Ke-Zhao Zhong
OBJECTIVE: The study aimed to explore the effects of B cell lymphoma-2 (Bcl-2)-modified bone marrow-derived mesenchymal stem cells (BMSCs) transplantation for the treatment of diabetes mellitus-induced erectile dysfunction (DMED) in a rat model. METHODS: The DMED rat model was successfully established. Thirty-six DMED rats were assigned into the Bcl-2-BMSCs, null-BMSCs, BMSCs and phosphate buffered saline (PBS) groups. Meanwhile, 9 normal rats injected with PBS were taken as the normal control group...
November 29, 2016: Urologia Internationalis
https://www.readbyqxmd.com/read/27891153/pahs-target-hematopoietic-linages-in-bone-marrow-through-cyp1b1-primarily-in-mesenchymal-stromal-cells-but-not-ahr-a-reconstituted-in-vitro-model
#12
Catherine M Rondelli, Michele Campaigne Larsen, Alhaji N'jai, Charles J Czuprynski, Colin R Jefcoate
7,12-Dimethylbenz(a)anthracene (DMBA) rapidly suppresses hematopoietic progenitors, measured as colony forming units (CFU), in mouse bone marrow (BM) leading to mature cell losses as replenishment fails. These losses are mediated by Cyp1b1, independent of the AhR, despite induction of Cyp1b1. BM mesenchymal progenitor cells (MPC) may mediate these responses since basal Cyp1b1 is minimally induced. PreB colony forming unit activity (PreB CFU) is lost within 24 hours in isolated BM cells (BMC) unless cocultured with cells derived from primary MPC (BMS2 line)...
2016: Stem Cells International
https://www.readbyqxmd.com/read/27890713/allogeneic-human-mesenchymal-stem-cells-in-patients-with-idiopathic-pulmonary-fibrosis-via-intravenous-delivery-aether-a-phase-i-safety-clinical-trial
#13
Marilyn K Glassberg, Julia Minkiewicz, Rebecca L Toonkel, Emmanuelle S Simonet, Gustavo A Rubio, Darcy Difede, Shirin Shafazand, Aisha Khan, Marietsy V Pujol, Vincent F LaRussa, Lisa H Lancaster, Glenn D Rosen, Joel Fishman, Yolanda N Mageto, Adam Mendizabal, Joshua M Hare
BACKGROUND: Despite recent FDA approval of two new drugs for idiopathic pulmonary fibrosis (IPF), curative therapies remain elusive and mortality remains high. Pre-clinical and clinical data support the safety of human mesenchymal stem cells as a potential novel therapy for this fatal condition. The AETHER trial was the first study designed to evaluate the safety of a single infusion of bone marrow-derived mesenchymal stem cells in patients with idiopathic pulmonary fibrosis. METHODS: Nine patients with mild to moderate IPF were sequentially assigned to one of three cohorts and dosed with a single intravenous infusion of 20, 100, or 200 x 10(6) human bone marrow-derived mesenchymal stem cells per infusion from young, unrelated, male donors...
November 24, 2016: Chest
https://www.readbyqxmd.com/read/27888602/a-glycovariant-of-human-cd44-is-characteristically-expressed-on-human-mesenchymal-stem-cells
#14
Gisela Pachón-Peña, Conor Donnelly, Catalina Ruiz-Cañada, Adam Katz, Sonia Fernández-Veledo, Joan Vendrell, Robert Sackstein
The clinical effectiveness of systemically-administered human mesenchymal stem cells (hMSCs) depends on their capacity to engage vascular endothelium. hMSCs derived from bone marrow (BM-hMSCs) natively lack endothelial binding capacity but express a CD44 glycovariant containing N-linked sialyllactosamines that can be α(1,3)-fucosylated using fucosyltransferase- VI (FTVI) to enforce sLe(X) decorations, thereby creating hematopoietic cell E-/L-selectin ligand (HCELL). HCELL expression programs potent shear-resistant adhesion of circulating cells to endothelial beds expressing E-selectin...
November 26, 2016: Stem Cells
https://www.readbyqxmd.com/read/27888550/mesenchymal-stem-stromal-cells-biology-and-preclinical-evidence-for-therapeutic-potential-for-organ-dysfunction-following-trauma-or-sepsis
#15
Michael A Matthay, Shibani Pati, Jae-Woo Lee
Several experimental studies have provided evidence that bone-marrow derived mesenchymal stem (stromal) cells (MSC) may be effective in treating critically ill surgical patients who develop traumatic brain injury, acute renal failure, or the acute respiratory distress syndrome. There is also preclinical evidence that MSC may be effective in treating sepsis-induced organ failure, including evidence that MSC have anti-microbial properties. This review considers preclinical studies with direct relevance to organ failure following trauma, sepsis or major infections that apply to critically ill patients...
November 26, 2016: Stem Cells
https://www.readbyqxmd.com/read/27883074/adipose-stem-cells-display-higher-regenerative-capacities-and-more-adaptable-electro-kinetic-properties-compared-to-bone-marrow-derived-mesenchymal-stromal-cells
#16
Ahmed El-Badawy, Marwa Amer, Reda Abdelbaset, Sameh N Sherif, Marwan Abo-Elela, Yehya H Ghallab, Hamdy Abdelhamid, Yehea Ismail, Nagwa El-Badri
Adipose stem cells (ASCs) have recently emerged as a more viable source for clinical applications, compared to bone-marrow mesenchymal stromal cells (BM-MSCs) because of their abundance and easy access. In this study we evaluated the regenerative potency of ASCs compared to BM-MSCs. Furthermore, we compared the dielectric and electro-kinetic properties of both types of cells using a novel Dielectrophoresis (DEP) microfluidic platform based on a printed circuit board (PCB) technology. Our data show that ASCs were more effective than BM-MSCs in promoting neovascularization in an animal model of hind-limb ischemia...
November 24, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27882356/intra-osseous-co-transplantation-of-cd34-selected-umbilical-cord-blood-and-mesenchymal-stromal-cells
#17
Leland Metheny, Saada Eid, Karen Lingas, Jane Reese, Howard Meyerson, Alexander Tong, Marcos de Lima, Alex Y Huang
Human mesenchymal stromal cells (MSC) have been shown to support the growth and differentiation of hematopoietic stem cells (HSC). We hypothesized that intra-osseous (IO) co-transplantation of MSC and umbilical cord blood (UCB) may be effective in improving early HSC engraftment, as IO transplantation has been demonstrated to enhance UCB engraftment in NOD SCID-gamma (NSG) mice. Following non-lethal irradiation (300rads), 6 groups of NSG mice were studied: 1) intravenous (IV) UCB CD34(+) cells, 2) IV UCB CD34(+) cells and MSC, 3) IO UCB CD34(+) cells, 4) IO UCB CD34(+) cells and IO MSC, 5) IO UCB CD34(+) cells and IV MSC, and 6) IV UCB CD34(+) and IO MSC...
2016: Hematol Med Oncol
https://www.readbyqxmd.com/read/27882169/efficacy-of-mesenchymal-stem-cells-in-treating-patients-with-osteoarthritis-of-the-knee-a-meta-analysis
#18
Gang-Hua Cui, Yang Yang Wang, Chang-Jun Li, Chen-Hui Shi, Wei-Shan Wang
To assess the clinical efficacy and safety of mesenchymal stem cell (MSC) treatment for osteoarthritis of the knee (KOA), a systematic electronic literature search was performed on PubMed, EMBASE and Web of Science. Studies published in English from the earliest record to December 2014 were searched using the following keywords: Cartilage defect, cartilage repair, osteoarthritis, KOA, stem cells, MSCs, bone marrow concentrate (BMC), adipose-derived mesenchymal stem cells, synovial-derived mesenchymal stem cells and peripheral blood-derived mesenchymal stem cells...
November 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/27881051/mechanisms-of-immune-suppression-utilized-by-canine-adipose-and-bone-marrow-derived-mesenchymal-stem-cells
#19
Lyndah Chow, Valerie Johnson, Jonathan W Coy, Daniel P Regan, Steven W Dow
Mesenchymal stem cells (MSC) from rodents and humans have been shown to suppress T cells by distinct primary pathways, with NO-dependent pathways dominating in rodents and IDO-dependent pathways dominating in humans. However, the immune suppressive pathways utilized by canine MSC have not been as thoroughly studied, nor have BM-MSC and Ad-MSC been directly compared for their immune modulatory potency or pathway utilization. Therefore, canine BM-MSC and Ad-MSC were generated in vitro and their potency in suppressing T cell proliferation and cytokine production was compared, as well as differential gene expression...
November 23, 2016: Stem Cells and Development
https://www.readbyqxmd.com/read/27878265/synergistic-effects-of-overexpression-of-bmp%C3%A2-2-and-tgf%C3%A2-%C3%AE-3-on-osteogenic-differentiation-of-bone-marrow-mesenchymal-stem-cells
#20
Yilin Wang, Tian He, Jie Liu, Hongzhi Liu, Lugang Zhou, Wei Hao, Yujie Sun, Xin Wang
Bone morphogenetic protein 2 (BMP-2) and transforming growth factor β (TGF-β) isoforms are important in advancing bone regeneration. The aim of the present study was to investigate the positive and reciprocal effect of TGF‑β3, one of the three TGF‑β isoforms, on BMP‑2 in promoting osteogenic differentiation. Exogenous BMP‑2 and TGF‑β3 genes were separately, and in combination, overexpressed in rabbit bone marrow‑derived mesenchymal stem cells (rBMSCs). Expression levels of BMP‑2 and TGF‑β3 were evaluated using reverse‑transcription‑polymerase chain reaction (RT‑PCR) and Western blotting assays...
November 22, 2016: Molecular Medicine Reports
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