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https://www.readbyqxmd.com/read/28468636/nationwide-molecular-epidemiology-of-methicillin-resistant-staphylococcus-aureus-responsible-for-horse-infections-in-france
#1
François Guérin, Marguerite Fines-Guyon, Pierrick Meignen, Géraldine Delente, Caroline Fondrinier, Nancy Bourdon, Vincent Cattoir, Albertine Léon
BACKGROUND: The epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) isolated in horse infections is not well documented, especially in France. The aim of the study was to evaluate the prevalence of MRSA isolates in horse infections from 2007 to 2013 in France and to characterize phenotypically and genotypically this collection. RESULTS: Out of 1393 S. aureus horse isolates, 85 (6.1%) were confirmed to be MRSA. Interestingly, the prevalence of MRSA significantly increased from 2007-2009 to 2010-2013 (0...
May 3, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28421046/horses-in-denmark-are-a-reservoir-of-diverse-clones-of-methicillin-resistant-and-susceptible-staphylococcus-aureus
#2
Md Zohorul Islam, Carmen Espinosa-Gongora, Peter Damborg, Raphael N Sieber, Rikke Munk, Louise Husted, Arshnee Moodley, Robert Skov, Jesper Larsen, Luca Guardabassi
Denmark is a country with high prevalence of livestock-associated methicillin-resistant Staphylococcus aureus (MRSA) clonal complex (CC) 398 in pigs. Even though pig farming is regarded as the main source of human infection or colonization with MRSA CC398, 10-15% of the human cases appear not to be linked to pigs. Following the recent reports of MRSA CC398 in horses in other European countries and the lack of knowledge on S. aureus carriage in this animal species, we carried out a study to investigate whether horses constitute a reservoir of MRSA CC398 in Denmark, and to gain knowledge on the frequency and genetic diversity of S...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28399155/frequent-isolation-of-methicillin-resistant-staphylococcus-aureus-mrsa-st398-among-healthy-pigs-in-portugal
#3
Teresa Conceição, Hermínia de Lencastre, Marta Aires-de-Sousa
BACKGROUND: Although livestock-associated ST398 methicillin-resistant Staphylococcus aureus (MRSA) has been widely reported in different geographic regions, MRSA carriage studies among healthy pigs in Portugal are very limited. METHODS AND FINDINGS: In total, 101 swine nasal samples from two Portuguese farms were screened for MRSA. In addition five swine workers (including one veterinary and one engineer) and four household members were nasally screened. The isolates were characterized by spa typing, SCCmec typing and MLST...
2017: PloS One
https://www.readbyqxmd.com/read/28298453/detection-of-mecc-positive-staphylococcus-aureus-what-to-expect-from-immunological-tests-targeting-pbp2a
#4
Céline Dupieux, Coralie Bouchiat, Anders Rhod Larsen, Bruno Pichon, Mark Holmes, Christopher Teale, Giles Edwards, Robert Hill, Jean-Winoc Decousser, Sophie Trouillet-Assant, Andreas Petersen, Robert Skov, Angela Kearns, Frédéric Laurent
Rapid identification of methicillin-resistant Staphylococcus aureus (MRSA) is pivotal for the control of their dissemination and for accurate management of infected patients.….
March 15, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28108681/re-emergence-of-methicillin-susceptibility-in-a-resistant-lineage-of-staphylococcus-aureus
#5
Alice Ledda, James R Price, Kevin Cole, Martin J Llewelyn, Angela M Kearns, Derrick W Crook, John Paul, Xavier Didelot
OBJECTIVES: MRSA is a leading cause of hospital-associated infection. Acquired resistance is encoded by the mecA gene or its homologue mecC, but little is known about the evolutionary dynamics involved in gain and loss of resistance. The objective of this study was to obtain an expanded understanding of Staphylococcus aureus methicillin resistance microevolution in vivo, by focusing on a single lineage. METHODS: We compared the whole-genome sequences of 231 isolates from a single epidemic lineage [clonal complex 30 (CC30) and spa-type t018] of S...
January 20, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28094151/comparison-of-the-bd-max-mrsa-xt-to-the-cepheid%C3%A2-xpert%C3%A2-mrsa-assay-for-the-molecular-detection-of-methicillin-resistant-staphylococcus-aureus-from-nasal-swabs
#6
COMPARATIVE STUDY
Sanjay R Mehta, Jasmine Estrada, Juan Ybarra, Joshua Fierer
INTRODUCTION: Variation in MRSA genotypes may affect the sensitivity of molecular assays to detect this organism. METHODS: We compared 2 commonly used screening assays, the Cepheid™ Xpert® MRSA and the BD MAX™ MRSA XT on consecutively obtained nasal swabs from 479 subjects. Specimens giving discordant results were subjected to additional microbiologic and molecular testing. RESULTS: Six hundred forty-two (97.6%) of the 658 test results were concordant...
April 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28087612/biofilm-formation-and-antimicrobial-resistance-genes-of-coagulase-negative-staphylococci-isolated-from-cows-with-mastitis-in-argentina
#7
Mariela E Srednik, Yannick D N Tremblay, Josée Labrie, Marie Archambault, Mario Jacques, Fernández Cirelli Alicia, Elida R Gentilini
Mastitis affects the health and welfare of dairy cows worldwide. Coagulase-negative staphylococci (CNS) are known to form biofilms and are increasingly recognized as a cause of persistent bovine intramammary infections. A total of 90 CNS isolated from cows with clinical and subclinical mastitis in Argentina from 2008-2014 were identified by PCR-RFLP using the gap gene. Standard microtiter plate assays were used to assess CNS biofilm formation and Staphylococcus haemolyticus species formed the strongest biofilms...
January 12, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28069659/full-genome-sequencing-identifies-in-the-genetic-background-several-determinants-that-modulate-the-resistance-phenotype-in-methicillin-resistant-staphylococcus-aureus-strains-carrying-the-novel-mecc-gene
#8
Catarina Milheiriço, Hermínia de Lencastre, Alexander Tomasz
Most methicillin-resistant Staphylococcus aureus (MRSA) strains are resistant to beta-lactam antibiotics due to the presence of the mecA gene, encoding an extra penicillin-binding protein (PBP2A) that has low affinity for virtually all beta-lactam antibiotics. Recently, a new resistance determinant-the mecC gene-was identified in S. aureus isolates recovered from humans and dairy cattle. Although having typically low MICs to beta-lactam antibiotics, MRSA strains with the mecC determinant are also capable of expressing high levels of oxacillin resistance when in an optimal genetic background...
March 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28063000/evaluation-of-loop-mediated-isothermal-amplification-for-the-rapid-identification-of-bacteria-and-resistance-determinants-in-positive-blood-cultures
#9
J Rödel, J A Bohnert, S Stoll, L Wassill, B Edel, M Karrasch, B Löffler, W Pfister
The use of molecular assays to rapidly identify pathogens and resistance genes directly from positive blood cultures (BCs) contribute to shortening the time required for the diagnosis of bloodstream infections. In this work, loop-mediated isothermal amplification (LAMP) assays have been examined for their potential use in BC diagnosis. Three different assays were applied. The commercially available eazyplex® MRSA test detects Staphylococcus aureus, S. epidermidis, mecA, and mecC. Two in-house assays [Gram-positive (GP) and Gram-negative (GN)] have been developed for the detection of streptococci, enterococci, vanA, vanB, Pseudomonas spp...
January 6, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28017539/staphylococcus-aureus-from-the-german-general-population-is-highly-diverse
#10
Karsten Becker, Frieder Schaumburg, Christian Fegeler, Alexander W Friedrich, Robin Köck
OBJECTIVES: This prospective cohort study evaluates colonization dynamics and molecular characteristics of methicillin-susceptible and - resistant Staphylococcus aureus (MSSA/MRSA) in a German general population. METHODS: Nasal swabs of 1878 non-hospitalized adults were screened for S. aureus. Participants were screened thrice in intervals of 6-8 months. Isolates were characterized by spa and agr typing, mecA and mecC possession, respectively, and PCRs targeting virulence factors...
January 2017: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/27992523/diversity-of-staphylococcus-aureus-isolates-in-european-wildlife
#11
Stefan Monecke, Dolores Gavier-Widén, Helmut Hotzel, Martin Peters, Sebastian Guenther, Alexandros Lazaris, Igor Loncaric, Elke Müller, Annett Reissig, Antje Ruppelt-Lorz, Anna C Shore, Birgit Walter, David C Coleman, Ralf Ehricht
Staphylococcus aureus is a well-known colonizer and cause of infection among animals and it has been described from numerous domestic and wild animal species. The aim of the present study was to investigate the molecular epidemiology of S. aureus in a convenience sample of European wildlife and to review what previously has been observed in the subject field. 124 S. aureus isolates were collected from wildlife in Germany, Austria and Sweden; they were characterized by DNA microarray hybridization and, for isolates with novel hybridization patterns, by multilocus sequence typing (MLST)...
2016: PloS One
https://www.readbyqxmd.com/read/27899074/antibiotic-resistance-and-clonal-diversity-of-invasive-staphylococcus-aureus-in-the-rural-ashanti-region-ghana
#12
Denise Dekker, Manuel Wolters, Eva Mertens, Kennedy Gyau Boahen, Ralf Krumkamp, Daniel Eibach, Norbert G Schwarz, Yaw Adu-Sarkodie, Holger Rohde, Martin Christner, Florian Marks, Nimako Sarpong, Jürgen May
BACKGROUND: Staphylococcus aureus is among the most common pathogens isolated from blood cultures in Ghana; yet the epidemiology of blood infections in rural settings is poorly described. This study aims to investigate antimicrobial susceptibility and clonal diversity of S. aureus causing bloodstream infections in two hospitals in the Ashanti Region, Ghana. METHODS: Blood cultures were performed for all febrile patients (≥37.5 °C) on hospital admission. Antibiotic susceptibility testing for S...
November 29, 2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27851997/methicillin-resistant-staphylococcus-aureus-among-animals-current-overview
#13
REVIEW
M Aires-de-Sousa
Currently, methicillin-resistant Staphylococcus aureus (MRSA) is a universal threat. After being well established in the healthcare setting, it has emerged in the community among people with no risk factors for MRSA acquisition, therefore imposing a new threat. The subsequent detection of MRSA colonizing or infecting animals as well as in food of animal origin was of major concern, revealing new reservoirs for MRSA. The major MRSA clonal lineages circulating in the different settings, i.e. in hospitals, in the community and among animals, are described here, differentiating between clones colonizing companion and food-chain animals...
November 13, 2016: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/27836760/staphylococcal-chromosomal-cassettes-mec-sccmec-a-mobile-genetic-element-in-methicillin-resistant-staphylococcus-aureus
#14
REVIEW
Junyan Liu, Dingqiang Chen, Brian M Peters, Lin Li, Bing Li, Zhenbo Xu, Mark E Shirliff
Considered to be a potential "superbug", methicillin-resistant Staphylococcus aureus (MRSA) has been one of the major recent infectious pathogens and thus poses a challenge to hospital infection control. The mobile genetic element staphylococcal chromosomal cassette mec (SCCmec) carries both the mecA or mecC gene, encoding for a novel specific penicillin-binding protein (PBP2a), and site-specific recombinase genes ccrAB or/and ccrC. In MRSA, the acquisition of SCCmec leads to the resistance to the β-lactam antibiotics...
December 2016: Microbial Pathogenesis
https://www.readbyqxmd.com/read/27834064/evaluation-of-bd-max-staph-sr-assay-for-differentiating-between-staphylococcus-aureus-and-coagulase-negative-staphylococci-and-determining-methicillin-resistance-directly-from-positive-blood-cultures
#15
Jaewoong Lee, Yeon Joon Park, Dong Jin Park, Kang Gyun Park, Hae Kyung Lee
BACKGROUND: We evaluated the performance of the BD MAX StaphSR Assay (SR assay; BD, USA) for direct detection of Staphylococcus aureus and methicillin resistance not only in S. aureus but also in coagulase-negative Staphylococci (CNS) from positive blood cultures. METHODS: From 228 blood culture bottles, 103 S. aureus [45 methicillin-resistant S. aureus (MRSA), 55 methicillin-susceptible S. aureus (MSSA), 3 mixed infections (1 MRSA+Enterococcus faecalis, 1 MSSA+MRCNS, 1 MSSA+MSCNS)], and 125 CNS (102 MRCNS, 23 MSCNS) were identified by Vitek 2...
January 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/27790966/epidemiology-and-microbiological-characterization-of-clinical-isolates-of-staphylococcus-aureus-in-a-single-healthcare-region-of-the-uk-2015
#16
C Horner, L Utsi, L Coole, M Denton
We investigated the epidemiology and characterization of isolates of Staphylococcus aureus within the Yorkshire and Humber (YH) region in the UK. In July 2015, each laboratory within YH (n = 14) was assigned two consecutive days during which all clinical isolates of S. aureus were collected. Isolates were tested for antibiotic susceptibilities and the presence of genes encoding methicillin resistance (mecA and mecC), Panton-Valentine leukocidin (PVL) (lukS-PV), and efflux-mediated chlorhexidine resistance (qacA); isolates were also characterized by spa-types...
January 2017: Epidemiology and Infection
https://www.readbyqxmd.com/read/27681906/staphylococcus-aureus-in-animals-and-food-methicillin-resistance-prevalence-and-population-structure-a-review-in-the-african-continent
#17
REVIEW
Carmen Lozano, Haythem Gharsa, Karim Ben Slama, Myriam Zarazaga, Carmen Torres
The interest about Staphylococcus aureus (S. aureus) and methicillin resistant S. aureus (MRSA) in livestock, and domestic and wild animals has significantly increased. The spread of different clonal complexes related to livestock animals, mainly CC398, and the recent description of the new mecC gene, make it necessary to know more about the epidemiology and population structure of this microorganism all over the world. Nowadays, there are several descriptions about the presence of S. aureus and/or MRSA in different animal species (dogs, sheep, donkeys, bats, pigs, and monkeys), and in food of animal origin in African countries...
February 4, 2016: Microorganisms
https://www.readbyqxmd.com/read/27650187/report-of-mecc-carrying-mrsa-in-domestic-swine
#18
Ø Angen, M Stegger, J Larsen, B Lilje, H Kaya, K S Pedersen, A Jakobsen, A Petersen, A R Larsen
OBJECTIVES: We unexpectedly identified MRSA isolates carrying mecC (mecC-MRSA) from a Danish swine farm located in eastern Zealand. The objective of the present study was to investigate the origin of these isolates and their genetic relatedness to other mecC-MRSA isolates from Zealand. METHODS: WGS was used to infer the phylogenetic relationship between 19 identified mecC-MRSA isolates from the swine farm and 34 additional epidemiologically unrelated human isolates from the same geographical region of Denmark...
January 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/27648947/diversity-of-sccmec-elements-in-staphylococcus-aureus-as-observed-in-south-eastern-germany
#19
Stefan Monecke, Lutz Jatzwauk, Elke Müller, Hedda Nitschke, Katharina Pfohl, Peter Slickers, Annett Reissig, Antje Ruppelt-Lorz, Ralf Ehricht
SCCmec elements are very important mobile genetic elements in Staphylococci that carry beta-lactam resistance genes mecA/mecC, recombinase genes and a variety of accessory genes. Twelve main types and a couple of variants have yet been described. In addition, there are also other SCC elements harbouring other markers. In order to subtype strains of methicillin-resistant S. aureus (MRSA) based on variations within their SCCmec elements, 86 markers were selected from published SCC sequences for an assay based on multiplexed primer extension reactions followed by hybridisation to the specific probes...
2016: PloS One
https://www.readbyqxmd.com/read/27634917/in-vitro-activity-of-ceftaroline-against-clinical-staphylococcus-aureus-isolates-collected-during-a-national-survey-conducted-in-belgian-hospitals
#20
M Angeles Argudín, M Dodémont, M Taguemount, S Roisin, R de Mendonça, A Deplano, C Nonhoff, O Denis
OBJECTIVES: The aim of this study was to estimate the in vitro activity of ceftaroline against clinical Staphylococcus aureus isolates collected during national surveillance in Belgian acute-care hospitals. Ceftaroline-resistant isolates were further investigated for their resistance mechanisms. METHODS: From October 2013 to March 2014, 155 laboratories of Belgian acute-care hospitals were invited to send to the National Reference Centre-Staphylococcus aureus (Belgium) up to five non-duplicate S...
January 2017: Journal of Antimicrobial Chemotherapy
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