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Mecc mrsa

Carmen Lozano, Haythem Gharsa, Karim Ben Slama, Myriam Zarazaga, Carmen Torres
The interest about Staphylococcus aureus (S. aureus) and methicillin resistant S. aureus (MRSA) in livestock, and domestic and wild animals has significantly increased. The spread of different clonal complexes related to livestock animals, mainly CC398, and the recent description of the new mecC gene, make it necessary to know more about the epidemiology and population structure of this microorganism all over the world. Nowadays, there are several descriptions about the presence of S. aureus and/or MRSA in different animal species (dogs, sheep, donkeys, bats, pigs, and monkeys), and in food of animal origin in African countries...
February 4, 2016: Microorganisms
Ø Angen, M Stegger, J Larsen, B Lilje, H Kaya, K S Pedersen, A Jakobsen, A Petersen, A R Larsen
OBJECTIVES: We unexpectedly identified MRSA isolates carrying mecC (mecC-MRSA) from a Danish swine farm located in eastern Zealand. The objective of the present study was to investigate the origin of these isolates and their genetic relatedness to other mecC-MRSA isolates from Zealand. METHODS: WGS was used to infer the phylogenetic relationship between 19 identified mecC-MRSA isolates from the swine farm and 34 additional epidemiologically unrelated human isolates from the same geographical region of Denmark...
September 20, 2016: Journal of Antimicrobial Chemotherapy
Stefan Monecke, Lutz Jatzwauk, Elke Müller, Hedda Nitschke, Katharina Pfohl, Peter Slickers, Annett Reissig, Antje Ruppelt-Lorz, Ralf Ehricht
SCCmec elements are very important mobile genetic elements in Staphylococci that carry beta-lactam resistance genes mecA/mecC, recombinase genes and a variety of accessory genes. Twelve main types and a couple of variants have yet been described. In addition, there are also other SCC elements harbouring other markers. In order to subtype strains of methicillin-resistant S. aureus (MRSA) based on variations within their SCCmec elements, 86 markers were selected from published SCC sequences for an assay based on multiplexed primer extension reactions followed by hybridisation to the specific probes...
2016: PloS One
M Angeles Argudín, M Dodémont, M Taguemount, S Roisin, R de Mendonça, A Deplano, C Nonhoff, O Denis
OBJECTIVES: The aim of this study was to estimate the in vitro activity of ceftaroline against clinical Staphylococcus aureus isolates collected during national surveillance in Belgian acute-care hospitals. Ceftaroline-resistant isolates were further investigated for their resistance mechanisms. METHODS: From October 2013 to March 2014, 155 laboratories of Belgian acute-care hospitals were invited to send to the National Reference Centre-Staphylococcus aureus (Belgium) up to five non-duplicate S...
September 15, 2016: Journal of Antimicrobial Chemotherapy
Alexandre Gaymard, Maxime Pichon, Michaël Degaud, Jason Tasse, Céline Dupieux, Frédéric Laurent
Methicillin-resistant Staphylococcus aureus (MRSA) is involved in community-acquired and nosocomial diseases. The means of MRSA transmission and dissemination in the community remain uncertain. Studies have shown that public transport systems could be a source of MRSA and may serve as a potential source for community-acquired MRSA infections. This study aimed to investigate MRSA contamination on Lyon's metropolitan network (Métro) in France. Hand-touched surfaces were sampled with sterile swabs (Transystem(®)) during a 1-day transversal study by collecting 50 samples in seven hub stations and two trains for each of the four Métro lines...
October 2016: International Journal of Antimicrobial Agents
Constanze Seidel, Sonja Peters, Erik Eschbach, Andrea T Feßler, Boris Oberheitmann, Stefan Schwarz
The gene mecA and its homologue mecC confer methicillin resistance in Staphylococcus aureus and other staphylococci. Methicillin-resistant staphylococci (MRS) are considered resistant to all β-lactam antibiotics. To avoid the use of β-lactam antibiotics for the control of MRS infections, there is an urgent need for a fast and reliable screening assay for mecA and mecC that can easily be integrated in routine laboratory diagnostics. The aim of this study was the development of such a rapid detection method for methicillin resistance based on nucleic acid lateral flow immunoassay (NALFIA) technology...
August 16, 2016: Veterinary Microbiology
Benedikt Hogan, Raphael Rakotozandrindrainy, Hassan Al-Emran, Denise Dekker, Andreas Hahn, Anna Jaeger, Sven Poppert, Hagen Frickmann, Ralf Matthias Hagen, Volker Micheel, Sabine Crusius, Jean Noel Heriniaina, Jean Philibert Rakotondrainiarivelo, Tsiriniaina Razafindrabe, Jürgen May, Norbert Georg Schwarz
BACKGROUND: Methicillin-resistant Staphylococcus aureus (MRSA) clones pose a significant threat to hospitalised patients because the bacteria can be transmitted by asymptomatic carriers within healthcare facilities. To date, nothing is known about the prevalence of S. aureus and MRSA among healthcare workers in Madagascar. The objective of our study was to examine the prevalence and clonal epidemiology of nasal S. aureus and MRSA among healthcare workers and non-medical University students in Antananarivo, Madagascar...
2016: BMC Infectious Diseases
Marc Armengol-Porta, Alberto Tenorio-Abreu, Dirk Bandt, David C Coleman, Dolores Gavier-Widen, Helmut Hotzel, Peter Kinnevey, Alexandros Lazaris, Martin Peters, Lena Rangstrup-Christensen, Katharina Schlotter, Anna C Shore, Ralf Ehricht, Stefan Monecke
Ceftaroline is a new cephalosporin with activity against methicillin-resistant Staphylococcus aureus (MRSA). A collection of 17 clinical and veterinary mecC-positive MRSA isolates was tested to evaluate the in vitro efficacy of ceftaroline against recently emerged mecC-MRSA isolates. Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of ceftaroline for the 17 isolates were determined by broth microdilution using the methodology and interpretive criteria of the Clinical and Laboratory Standards Institute (CLSI)...
June 2016: Journal of Global Antimicrobial Resistance
Xiaojuan Yang, Jumei Zhang, Shubo Yu, Qingping Wu, Weipeng Guo, Jiahui Huang, Shuzhen Cai
Staphylococcus aureus, particularly methicillin-resistant S.aureus (MRSA), is a life-threatening pathogen in humans, and its presence in food is a public health concern. MRSA has been identified in foods in China, but little information is available regarding MRSA in ready-to-eat (RTE) foods. We aimed to investigate the prevalence of S. aureus and MRSA in Chinese retail RTE foods. All isolated S. aureus were tested for antimicrobial susceptibility, and MRSA isolates were further characterized by multilocus sequence typing (MLST) and staphylococcal cassette chromosome mec (SCCmec) typing...
2016: Frontiers in Microbiology
S M Hatcher, K W Myers, C D Heaney, J Larsen, D Hall, M B Miller, J R Stewart
Industrial hog operations (IHOs) have been identified as a source of antibiotic-resistant Staphylococcus aureus, including methicillin-resistant S. aureus (MRSA). However, few studies have investigated the presence of antibiotic-resistant S. aureus in the environment near IHOs, specifically surface waters proximal to spray fields where IHO liquid lagoon waste is sprayed. Surface water samples (n=179) were collected over the course of approximately one year from nine locations in southeastern North Carolina and analyzed for the presence of presumptive MRSA using CHROMagar MRSA media...
September 15, 2016: Science of the Total Environment
Kate A Worthing, Geoffrey W Coombs, Stanley Pang, Sam Abraham, Sugiyono Saputra, Darren J Trott, David Jordan, Hui San Wong, Rebecca J Abraham, Jacqueline M Norris
No abstract text is available yet for this article.
August 2016: Journal of Antimicrobial Chemotherapy
A-K Lindgren, E Gustafsson, A C Petersson, E Melander
In 2011, a novel mecA gene homologue, mecC, was reported in isolates from both humans and dairy cattle. The epidemiology of mecC methicillin-resistant Staphylococcus aureus (MRSA) in humans is not yet well known. In this retrospective study, we present the epidemiology of human clinical cases with mecC MRSA detected in the southern part of Sweden during the period 2005-2014. A total of 45 patients with an isolate positive for mecC MRSA were included in the study. Twenty-six isolates were found before 2012 and were retrospectively tested for mecC...
June 2016: European Journal of Clinical Microbiology & Infectious Diseases
Enas Mamdouh Hefzy, Gamal Mohamedin Hassan, Fadwa Abd El Reheem
BACKGROUND: Transmission of methicillin-resistant Staphylococcus aureus (MRSA) among patients is linked mainly to health care personnel. The Panton-Valentine leukocidin (PVL) is a cytotoxin that causes leukocyte lysis. Virulence of pvl-positive-MRSA has been attributed to its ability to express PVL toxin. METHODS: Swabs for detection of nasal carriage of pvl-positive MRSA among health care personnel at Fayoum University Hospital, Fayoum, Egypt, were collected from 223 health care personnel including 70 doctors (31...
June 2016: Surgical Infections
Funmilola Abidemi Ayeni, Tombari Gbarabon, Camilla Andersen, Niels Norskov-Lauritsen
BACKGROUND: Staphylococcus aureus is often responsible for fatal infections and recent upsurge of resistant strains has resulted in therapeutic failure. The identification of this microorganism is a major challenge to medical microbiologists in developing countries. METHODS: One hundred and eighty five isolates which had been previously isolated from the nares of 185 healthy college students' volunteers in Amassoma, Bayelsa State, South Nigeria were identified by MALDI TOF mass spectrometry, and PCR amplification of the spa gene...
December 2015: African Health Sciences
Eleanna Drougka, Antigoni Foka, Christos K Koutinas, Eleni Jelastopulu, Nikolaos Giormezis, Ourania Farmaki, Styliani Sarrou, Evangelos D Anastassiou, Efthimia Petinaki, Iris Spiliopoulou
Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) prevalence among companion animals and veterinary personnel (VP) was investigated. Strains' molecular characteristics were evaluated in order to assess S. aureus transmission. Specimens (224) from colonized and infected sites of 102 animals (92 dogs, 10 cats) and 18 VP were collected during 2012 and 2013. Antibiotic susceptibility was performed by the disk diffusion method and Etest. mecA, mecC, tst (toxic shock syndrome toxin) and lukF/lukS-PV (Panton-Valentine leukocidin, PVL) genes were investigated by PCR...
April 1, 2016: Preventive Veterinary Medicine
Natacha Couto, Cláudia Monchique, Adriana Belas, Cátia Marques, Luís T Gama, Constança Pomba
OBJECTIVES: The objective of this study was to investigate the evolution of resistance to antimicrobials, corresponding mechanisms and molecular characteristics of Staphylococcus spp., between 1999 and 2014. METHODS: Susceptibility to 38 antimicrobials was determined for 632 clinical staphylococcal isolates obtained from companion animals (dogs, cats, horses and other animals). Twenty antimicrobial resistance genes, including mecA and mecC, were screened by PCR...
June 2016: Journal of Antimicrobial Chemotherapy
Ana Vindel, Emilia Cercenado
No abstract text is available yet for this article.
May 2016: Enfermedades Infecciosas y Microbiología Clínica
Sandra Aedo, Alexander Tomasz
Resistance to beta-lactam antibiotics in methicillin-resistantStaphylococcus aureus(MRSA) requires the presence of an acquired genetic determinant,mecAormecC, which encode penicillin-binding protein PBP2A or PBP2A', respectively. Although all MRSA strains share a mechanism of resistance, the phenotypic expression of beta-lactam resistance shows considerable strain-to-strain variation. The stringent stress response, a stress response that results from nutrient limitation, was shown to play a key role in determining the resistance level of an MRSA strain...
April 2016: Antimicrobial Agents and Chemotherapy
Torsten Semmler, Ewan M Harrison, Antina Lübke-Becker, Rainer G Ulrich, Lothar H Wieler, Sebastian Guenther, Ivonne Stamm, Anne-Merethe Hanssen, Mark A Holmes, Szilvia Vincze, Birgit Walther
INTRODUCTION: Horizontal gene transfer (HGT) is an important driver for resistance- and virulence factor accumulation in pathogenic bacteria such as Staphylococcus aureus. METHODS: Here, we have investigated the downstream region of the bacterial chromosomal attachment site (attB) for the staphylococcal cassette chromosome mec (SCCmec) element of a commensal mecC-positive Staphylococcus stepanovicii strain (IMT28705; ODD4) with respect to genetic composition and indications of HGT...
2016: PloS One
Fernanda Fernandes dos Santos, Letícia Caldas Mendonça, Daniele Ribeiro de Lima Reis, Alessandro de Sá Guimarães, Carla Christine Lange, João Batista Ribeiro, Marco Antonio Machado, Maria Aparecida Vasconcelos Paiva Brito
Bacteria of the genus Staphylococcus are one of the major pathogens causing bovine mastitis. In recent decades, resistance of this genus to oxacillin (methicillin) has been a matter of concern due to the possibility of reducing the effectiveness of mastitis treatments and the transfer of resistance determinants to other bacteria. Oxacillin resistance was studied in 170 staphylococci from bovine milk samples, including 79 Staphylococcus aureus and 91 coagulase-negative staphylococci (CNS). The susceptibility profile of 10 antimicrobial agents used in veterinary practice was determined by the Etest method...
February 2016: Journal of Dairy Science
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