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https://www.readbyqxmd.com/read/27906145/nitrobacter-sp-extract-mediated-biosynthesis-of-ag2o-nps-with-excellent-antioxidant-and-antibacterial-potential-for-biomedical-application
#1
Gopalu Karunakaran, Matheswaran Jagathambal, Alexander Gusev, Nguyen Van Minh, Evgeny Kolesnikov, Arup Ratan Mandal, Denis Kuznetsov
In this study, extracellular extract of plant growth promoting bacterium, Nitrobacter sp. is used for the bioconversion of AgNO3 (silver nitrate) into Ag2O (silver oxide nanoparticles). It is an easy, ecofriendly and single step method for Ag2O NPs synthesis. The bio-synthesized nanoparticles were characterized using different techniques. UV-Vis results showed the maximum absorbance around 450 nm. XRD result shows the particles to have faced centered cubic (fcc) crystalline nature. FTIR analysis reveals the functional groups that are involved in bioconversion such as C-N, N-H and C=O...
December 2016: IET Nanobiotechnology
https://www.readbyqxmd.com/read/27906141/green-synthesis-of-zno-nanoparticles-by-olive-olea-europaea
#2
Shahla Hashemi, Zahra Asrar, Shahram Pourseyedi, Nazi Nadernejad
Green synthesis of nanoparticles is superior to physical and chemical methods as it is environment-friendly and cost-effective. The present study was carried out for inducing nanoparticles synthesis by zinc nitrate in the leaves extracts of olive. Further leaves extracts were evaluated for antiradical scavenging activity by 1, 1-diphenyl-2-picryl-hydrazyl assay. Morphological and structural properties of the synthesised ZnO nanoparticles have been characterised using UV-Vis spectrophotometer, FTIR, TEM, XRD and dynamic light scattering (DLS) analysis...
December 2016: IET Nanobiotechnology
https://www.readbyqxmd.com/read/27906136/rapid-synthesis-of-silver-nanoparticles-by-pseudomonas-stutzeri-isolated-from-textile-soil-under-optimised-conditions-and-evaluation-of-their-antimicrobial-and-cytotoxicity-properties
#3
Nishant Rajora, Sanket Kaushik, Anupam Jyoti, Shanker L Kothari
Present study utilised textile soil isolated bacterium Pseudomonas stutzeri to synthesise extracellular silver nanoparticles (AgNPs) under optimised conditions. The synthesised AgNPs were characterised using ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM). Optimisation showed AgNPs synthesis within 8 h using 2mM Ag nitrate at pH9, temperature 80°C and maximum absorbance toward 400 nm. TEM analysis revealed spherical shape AgNPs and reduction in size upto 8 nm was observed under optimised conditions...
December 2016: IET Nanobiotechnology
https://www.readbyqxmd.com/read/27904648/editing-of-the-urease-gene-by-crispr-cas-in-the-diatom-thalassiosira-pseudonana
#4
Amanda Hopes, Vladimir Nekrasov, Sophien Kamoun, Thomas Mock
BACKGROUND: CRISPR-Cas is a recent and powerful addition to the molecular toolbox which allows programmable genome editing. It has been used to modify genes in a wide variety of organisms, but only two alga to date. Here we present a methodology to edit the genome of Thalassiosira pseudonana, a model centric diatom with both ecological significance and high biotechnological potential, using CRISPR-Cas. RESULTS: A single construct was assembled using Golden Gate cloning...
2016: Plant Methods
https://www.readbyqxmd.com/read/27903988/metabolic-syndrome-in-relation-to-risk-of-meningioma
#5
Corinna Seliger, Christoph R Meier, Claudia Becker, Susan S Jick, Martin Proescholdt, Ulrich Bogdahn, Peter Hau, Michael F Leitzmann
BACKGROUND: Meningioma is a frequent primary intracranial tumor, the etiology of which is potentially related to adiposity. Metabolic syndrome (MetS) is an increasingly common disease characterized by having at least three of the following conditions: central adiposity, arterial hypertension, dyslipidemia, and insulin resistance. Only one prior study investigated MetS in relation to meningioma risk and found a positive association between the two. RESULTS: Among 2,027 cases and 20,269 controls, body mass index was positively associated with meningioma (p-value for trend < 0...
November 26, 2016: Oncotarget
https://www.readbyqxmd.com/read/27903568/effects-of-trans-endocardial-delivery-of-bone-marrow-derived-cd133-cells-on-left-ventricle-perfusion-and-function-in-patients-with-refractory-angina-final-results-of-randomized-double-blinded-placebo-controlled-regent-vsel-trial
#6
Wojciech Wojakowski, Tomasz Jadczyk, Aleksandra Michalewska-Wludarczyk, Zofia Parma, Miroslaw Markiewicz, Wojciech Rychlik, Magdalena Kostkiewicz, Katarzyna Gruszczynska, Anna Blach, Monika Dzierzak-Mietla, Wojciech Wanha, Joanna Ciosek, Beata Ochala, Lukasz Rzeszutko, Wieslaw Cybulski, Lukasz Partyka, Wojciech Zasada, Witold Wludarczyk, Sebastian Dworowy, Waclaw B Kuczmik, Grzegorz Smolka, Tomasz Z Pawlowski, Andrzej Ochala, Michal Tendera
RATIONALE: New therapies for refractory angina are needed. OBJECTIVE: Assessment of trans-endocardial delivery of bone marrow (BM) CD133(+)cells in patients with refractory angina. METHODS AND RESULTS: Randomized, double-blinded, placebo-controlled trial enrolled 31 patients with recurrent CCS II-IV angina despite optimal medical therapy, ≥ one myocardial segment with inducible ischemia in Tc-99m SPECT who underwent BM biopsy and were allocated to cells (n=16) or placebo (n=15)...
November 30, 2016: Circulation Research
https://www.readbyqxmd.com/read/27902337/thermodesulfitimonas-autotrophica-gen-nov-sp-nov-a-thermophilic-obligate-sulfite-reducing-bacterium-isolated-from-a-terrestrial-hot-spring
#7
G B Slobodkina, R V Baslerov, A A Novikov, E A Bonch-Osmolovskaya, A I Slobodkin
A novel thermophilic, anaerobic, chemolithoautotrophic bacterium, strain SF97T, was isolated from a terrestrial hot spring (Kuril Islands, Russia). Cells of strain SF97T were rod-shaped and motile with a Gram-positive cell-wall type. The novel isolate grew at 45-72oC (optimum 65oC) and pH 5.5-8.5 (optimum 6.0-6.5). The strain grew chemolithoautotrophically with molecular hydrogen as electron donor, sodium sulfite or SO2 gas as electron acceptor and bicarbonate/CO2 as a carbon source. Sulfate, thiosulfate, elemental sulfur, Fe(III) or nitrate were not used as an electron acceptors either with H2 or organic electron donors...
October 27, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902327/microbaculum-marinum-gen-nov-sp-nov-isolated-from-deep-seawater
#8
Yue Su, Shuaibo Han, Ruijun Wang, Xiaoyun Yu, Geyi Fu, Can Chen, Chongya Zhang, Yu Li, Cong Sun, Min Wu
A Gram-stain-negative, aerobic, rod-shaped and non-motile bacterium, designated HSF11T, was isolated from deep seawater of the South China Sea. Growth was observed at 20-40 °C (optimum, 35 °C), pH 5.0-8.5 (optimum, 7.0-7.5) and with 0-7 % NaCl (optimum, 2 %). Bacteriochlorophyll a and poly-β-hydroxybutyrate (PHB) granules were not detected. Nitrate could be reduced to nitrite. The major fatty acids (≥5 %) of strain HSF11T were C19:0 cyclo ω8c, C18:0 and C16:0. The isoprenoid quinone was Q-10. The total lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), two unknown phospholipids (PL1, PL2), one unknown aminolipid (AL1) and three unknown lipids (L1, L3, L4)...
November 8, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902275/arcticibacterium-luteifluviistationis-gen-nov-sp-nov-isolated-from-arctic-seawater
#9
Dan-Dan Li, Ming Peng, Ning Wang, Xiu-Juan Wang, Xi-Ying Zhang, Xiu-Lan Chen, Hai-Nan Su, Yu-Zhong Zhang, Mei Shi
A Gram-staining-negative, aerobic, non-motile and yellow-pigmented bacterium, designated strain SM1504T, was isolated from Arctic seawater. It hydrolyzed aesculin and gelatin but did not reduce nitrate to nitrite. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain SM1504T constituted a distinct phylogenetic line within the family Cytophagaceae and was closely related to species of the genera Lacihabitans, Emticicia, Fluviimonas and Leadbetterella, with respect to which low sequence similarities between 88...
November 24, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902199/marinomonas-blandensis-sp-nov-a-novel-marine-gammaproteobacterium
#10
David R Arahal, Teresa Lucena, M Carmen Macián, María A Ruvira, José M González, Itziar Lekumberri, Jarone Pinhassi, María J Pujalte
A novel Gram-staining-negative, chemoorganotrophic, moderately halophilic, strictly aerobic bacterium, strain MED121T, was isolated from a seawater sample collected at the Blanes Bay Microbial Observatory in the north-western Mediterranean Sea. Analysis of its 16S rRNA gene sequence, retrieved from the whole-genome sequence, showed that this bacterium was most closely related to Marinomonas dokdonensis, and other Marinomonas spp. (96.3 % and 93.3-95.7 % sequence similarities, respectively), within the family Oceanospirillaceae...
October 9, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27901641/light-triggered-selective-nitration-of-psbo1-in-isolated-arabidopsis-thylakoid-membranes-is-inhibited-by-photosynthetic-electron-transport-inhibitors
#11
Misa Takahashi, Jun Shigeto, Atsushi Sakamoto, Hiromichi Morikawa
PSBO1 is exclusively nitrated when isolated thylakoid membranes are incubated in a buffer bubbled with nitrogen dioxide (NO2) containing NO2 and nitrite. NO2 is the primary intermediate for this selective nitration. Isolated thylakoid membranes were incubated in NO2-bubbled buffer at 25°C in the light or dark. Protein analysis confirmed the selective nitration of PSBO1. Illumination was found to be essential in PSBO1 nitration. A nitration mechanism whereby nitratable tyrosine residues of PSBO1 are, prior to nitration, selectively photo-oxidized by photosynthetic electron transport to tyrosyl radicals to combine with NO2 to form 3-nitrotyrosine was hypothesized...
November 30, 2016: Plant Signaling & Behavior
https://www.readbyqxmd.com/read/27900831/cooperation-of-three-chromophores-generates-the-water-resistant-nitrate-nonlinear-optical-material-bi3-teo6-oh-no3-2
#12
Sangen Zhao, Yi Yang, Yaoguo Shen, Bingqing Zhao, Lina Li, Chengmin Ji, Zhenyue Wu, Daqiang Yuan, Zheshuai Lin, Maochun Hong, Junhua Luo
Nitrates have long been ignored for practical uses as nonlinear optical (NLO) materials because they are usually very easy to dissolve in water; despite this, the π-conjugated [NO3 ](-) is among the most desirable NLO-active structural units. The cooperation of three structural chromophores, namely, Bi3 O6 OH short chains with 6s(2) lone pair electrons, distorted TeO6 octahedra with d(10) electrons, and π-conjugated [NO3 ](-) triangles, generates a new nitrate NLO material, Bi3 TeO6 OH(NO3 )2 , which exhibits an enhanced phase-matchable NLO response of three times that of KH2 PO4 (3×KH2 PO4 ), exceeding those of most nitrate NLO materials...
November 30, 2016: Angewandte Chemie
https://www.readbyqxmd.com/read/27900721/effect-of-initial-ph-and-ph-adjusted-acid-on-nutrient-recovery-from-hydrolysis-urine-by-combining-acidification-with-evaporation-crystallization
#13
Shanqing Jiang, Xiaochang Wang, Shengjiong Yang, Honglei Shi
Nutrient recovery from human urine is a promising pretreatment of domestic wastewater and provides a sustainable recyclability of nitrogen (N), phosphorus (P), and potassium (K). In this study, we present a method to recover all nutrients from hydrolysis urine (HU) in the form of solid products by combining acidification with evaporation-crystallization. The effect of initial pH (pHinit.) on N retention was investigated, and the optimal pHinit. was further determined by analysis of N retention efficiency. Additionally, crystallization process and product composition based on different pH-adjusted acids were also compared...
November 30, 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/27900610/novel-sgc-stimulators-and-sgc-activators-for-the-treatment-of-heart-failure
#14
Stefanie Breitenstein, Lothar Roessig, Peter Sandner, Kelly S Lewis
The burden of heart failure (HF) increases worldwide with an aging population, and there is a high unmet medical need in both, heart failure with reduced ejection fraction (HFrEF) and with preserved ejection fraction (HFpEF). The nitric oxide (NO) pathway is a key regulator in the cardiovascular system and modulates vascular tone and myocardial performance. Disruption of the NO-cyclic guanosine monophosphate (cGMP) signaling axis and impaired cGMP formation by endothelial dysfunction could lead to vasotone dysregulation, vascular and ventricular stiffening, fibrosis, and hypertrophy resulting in a decline of heart as well as kidney function...
November 30, 2016: Handbook of Experimental Pharmacology
https://www.readbyqxmd.com/read/27900590/assessment-of-oxidative-nitrative-modifications-of-plasma-proteins-selected-rotem-parameters-and-kinetics-of-fibrinogen-polymerization-in-patients-with-multiple-myeloma-at-diagnosis
#15
W Nowak, J Treliński, K Chojnowski, J Matczak, M Robak, M Misiewicz, P Nowak
Patients with multiple myeloma (MM) are at increased risk of thrombosis. Growing evidence indicates that oxidative and nitrative modifications of proteins, including fibrinogen, may lead to changes in hemostasis. The study compares samples from patients with MM at diagnosis and healthy volunteers with regard to the oxidative/nitrative modifications of proteins, ROTEM and thrombin-catalyzed fibrin polymerization. The content of carbonyl groups in plasma proteins of patients with MM was significantly higher than in controls (2...
January 2017: Medical Oncology
https://www.readbyqxmd.com/read/27898900/effects-of-stocking-rate-forage-management-and-grazing-management-on-performance-and-economics-of-cow-calf-production-in-southwest-arkansas
#16
P A Beck, C B Stewart, M B Sims, M S Gadberry, J A Jennings
The objective this research was to determine the effect of application of multiple grazing management practices at 2 stocking rates (SR) on the productivity and economics of the cow-calf enterprise in the Southeastern United States over a 4-yr period. Pasture management systems included: continuous grazing management at a moderate SR (0.8 ha/cow; CG) without additional forage management, rotational grazing management at a moderate SR (0.8 ha/cow (MR) with addition of stockpiled bermudagrass [ (L.) Pers.] and complementary cool season annuals, and rotational grazing management similar to MR but with a high SR (0...
September 2016: Journal of Animal Science
https://www.readbyqxmd.com/read/27898792/soybean-area-and-baseflow-driving-nitrate-in-iowa-s-raccoon-river
#17
Gabriele Villarini, Christopher S Jones, Keith E Schilling
Improved understanding of the drivers of stream nitrate is necessary to improve water quality. This is particularly true for Iowa, a large contributor to Mississippi River Basin nitrate loads. Here, we focus on the Raccoon River at Des Moines, Iowa, and develop statistical models to describe the monthly (from March to August) nitrate concentrations in terms of eight drivers representing monthly climate, monthly hydrology, and yearly cropping practices. We consider six two-parameter distributions, linear and nonlinear dependencies between the predictors, and the distributions' parameters...
November 2016: Journal of Environmental Quality
https://www.readbyqxmd.com/read/27898788/field-nitrogen-losses-induced-by-application-timing-of-digestate-from-dairy-manure-biogas-production
#18
Emily A Schwager, Andrew C VanderZaag, Claudia Wagner-Riddle, Anna Crolla, Chris Kinsley, Ed Gregorich
Anaerobic digestion of dairy manure has environmental benefits, but the impact of effluent (i.e., digestate [DG]) application on environmental nitrogen (N) losses from soils has not been well quantified. Our objective was to evaluate how field application of DG affected nitrous oxide (NO) emissions and nitrate (NO) leaching compared with raw dairy manure (RM) in spring versus fall applications. We measured N losses year-round for 2.5 yr in silage corn on tile-drained clay soil in Alfred, Ontario, Canada. Treatments were: digestate applied in spring (DS) and fall (DF), raw dairy manure applied in spring (RS) and fall (RF), urea applied in spring, and a control...
November 2016: Journal of Environmental Quality
https://www.readbyqxmd.com/read/27898778/drainage-and-nitrate-leaching-from-artificially-drained-maize-fields-simulated-by-the-precision-nitrogen-management-model
#19
Rebecca D Marjerison, Jeff Melkonian, John L Hutson, Harold M van Es, Shai Sela, Larry D Geohring, Jeffrey Vetsch
Environmental nitrogen (N) losses (e.g., nitrate leaching, denitrification, and ammonia volatilization) frequently occur in maize ( L.) agroecosystems. Decision support systems, designed to optimize the application of N fertilizer in these systems, have been developed using physically based models such as the Precision Nitrogen Management (PNM) model of soil and crop processes, which is an integral component of Adapt-N, a decision support tool providing N fertilizer recommendations for maize production. Such models can also be used to estimate N losses associated with particular management practices and over a range of current climates and future climate projections...
November 2016: Journal of Environmental Quality
https://www.readbyqxmd.com/read/27898774/effects-of-feeding-encapsulated-nitrate-to-beef-cattle-on-ammonia-and-greenhouse-gas-emissions-from-their-manure-in-a-short-term-manure-storage-system
#20
Chanhee Lee, Rafael C Araujo, Karen M Koenig, Michael L Hile, Eileen E Fabian-Wheeler, Karen A Beauchemin
A study was conducted to investigate effects of feeding encapsulated nitrate (EN) to beef cattle on ammonia (NH) and greenhouse gas emissions from their manure. Eight beef heifers were randomly assigned to diets containing 0 (control), 1, 2, or 3% EN (55% forage dry matter; EN replaced encapsulated urea in the control diet and therefore all diets were iso-nitrogenous) in a replicated 4 × 4 Latin square design. Urine and feces collected from individual animals were reconstituted into manure and incubated over 156 h using a steady-state flux chamber system to monitor NH, methane (CH), carbon dioxide (CO), and nitrous oxide (NO) emissions...
November 2016: Journal of Environmental Quality
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