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https://www.readbyqxmd.com/read/28226420/posnar2-1-osnar2-1-expression-enhances-nitrogen-uptake-efficiency-and-grain-yield-in-transgenic-rice-plants
#1
Jingguang Chen, Xiaoru Fan, Kaiyun Qian, Yong Zhang, Miaoquan Song, Yu Liu, Guohua Xu, Xiaorong Fan
The nitrate (NO3(-) ) transporter has been selected as an important gene maker in the process of environmental adoption in rice cultivars. In this work, we transfered another native OsNAR2.1 promoter with driving OsNAR2.1 gene into rice plants. The transgenic lines with exogenous pOsNAR2.1:OsNAR2.1 constructs showed enhanced OsNAR2.1 expression level, compared with wild type (WT), and (15) N influx in roots increased 21-32% in response to 0.2 mM and 2.5 mM (15) NO3(-) and 1.25 mM (15) NH4(15) NO3 . Under these three N conditions, the biomass of the pOsNAR2...
February 22, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28226188/effects-of-melatonin-on-seedling-growth-mineral-nutrition-and-nitrogen-metabolism-in-cucumber-under-nitrate-stress
#2
Ruimin Zhang, Yunkuo Sun, Zeyu Liu, Wen Jin, Yan Sun
In China, excessive use of nitrogen fertilizers in greenhouses leads to nitrate accumulations in soil and plants, which then limits productivity. Melatonin, an evolutionarily highly conserved molecule, has a wide range of functions in plants. We analyzed the effects of melatonin pretreatment on the growth, mineral nutrition, and nitrogen metabolism in cucumber (Cucumis sativus L. 'Jin You No. 1') when seedlings were exposed to nitrate stress. An application of 0.1 mM melatonin significantly improved the growth of plants and reduced their susceptibility to damage due to high nitrate levels (0...
February 22, 2017: Journal of Pineal Research
https://www.readbyqxmd.com/read/28225540/antituberculosis-drug-resistance-in-pulmonary-isolates-of-mycobacterium-tuberculosis-cuba-2012-2014
#3
Dihadenys Lemus, Miguel Echemendía, Raúl Díaz, María J Llanes, Lourdes Suárez, Antonio Marrero
INTRODUCTION Systematic surveillance of antituberculosis drug resistance allows identification of multidrug-resistant and extensively drug-resistant isolates of Mycobacterium tuberculosis. Surveillance studies of antituberculosis drug resistance systematically conducted in Cuba for over 15 years have revealed low circulation of multidrug-resistant tuberculosis, under 1% in new cases. OBJECTIVE Characterize antituberculosis drug resistance in isolates of M. tuberculosis recovered from patients with pulmonary tuberculosis in Cuba in 2012-2014...
January 2017: MEDICC Review
https://www.readbyqxmd.com/read/28225185/preventive-effects-of-topical-washing-with-miconazole-nitrate-containing-soap-to-diaper-candidiasis-in-hospitalized-elderly-patients-a-prospective-double-blind-placebo-controlled-study
#4
Hidenori Takahashi, Noritaka Oyama, Izumi Tanaka, Michiko Hasegawa, Kaori Hirano, Chieko Shimada, Minoru Hasegawa
The objective of the present randomized, double-blind trial was to evaluate the efficacy and safety of daily washing with miconazole nitrate-containing soap for candidiasis at diaper-covered sites in elderly subjects under long-term inpatient care. To confirm the onset and disappearance of candidiasis, we microscopically evaluated the existence of the pseudohyphae and/or blastoconidia of Candida spp. We enrolled 75 elderly patients who wore diapers all day in the hospital or nursing home. Patients were randomly assigned to receive treatment with either miconazole soap or miconazole-free placebo soap, and 28 patients in the miconazole group and 27 patients in the placebo group were followed for 4 weeks...
February 22, 2017: Journal of Dermatology
https://www.readbyqxmd.com/read/28225126/carborane-layered-double-hydroxide-nanohybrids-for-potential-targeted-and-magnetically-targeted-bnct-applications
#5
Ahmet Nedim Ay, Hande Akar, Adnana Zaulet, Clara Viňas, Francesc Teixidor, Birgul Zumreoglu-Karan
Carborane-intercalated layered double hydroxide nanohybrids (CB-LDH) and a magnesium ferrite (MF) supported-CB-LDH core-shell nanocomposite (CB-LDH@MF) are reported. The preparation of nanohybrids were carried out by exchanging the interlayer nitrate groups of Mg-Al-NO3-LDH with monothiol-o-carborane (SCB), 1-methyl-2-carboxyl-o-carborane (MeCB), 1-phenyl-2-carboxyl-o-carborane (PhCB) and 1,12-dicarboxyl-p-carborane (COOHCB) molecules. A magnetic core-shell nanocomposite was further prepared by supporting the COOHCB-LDH nanohybrid on MF nanoparticles...
February 22, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28224156/binding-of-ligands-containing-carbonyl-and-phenol-groups-to-iron-iii-new-fe6-fe10-and-fe12-coordination-clusters
#6
Alexandros A Kitos, Constantina Papatriantafyllopoulou, Anastasios J Tasiopoulos, Spyros P Perlepes, Albert Escuer, Vassilios Nastopoulos
The initial use of ligands 2'-hydroxyacetophenone (HL(1)), 2-hydroxybenzophenone (HL(2)) and 2,2'-dihydroxybenzophenone (H2L(3)) in iron(iii) chemistry is described. The syntheses and crystal structures are reported for five iron(iii) clusters: [Fe10O4(OMe)14(L(1))6(MeOH)2](NO3)2·3MeOH (1·3MeOH), [Fe12O4(OH)(OMe)17(L(1))8](ClO4)2·2H2O (2·2H2O), [Fe10O4(OMe)14Cl4(L(2))4(MeOH)2] (3), [Fe10O4(OMe)14(L(2))6(py)2](ClO4)2·MeOH (4·MeOH), where py = pyridine, and [Fe6O2(OEt)6(O2CMe)2(L(3))2(HL(3))2] (5). The molecular structures of the decanuclear clusters 1, 3 and 4 are organized around a {Fe10(μ4-O)4(μ3-OMe)2(μ-OMe)12}(8+) core consisting of ten {Fe3O4} face-sharing defective cubane units...
February 22, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28224117/perc-manipulates-metabolism-and-surface-antigens-in-enteropathogenic-escherichia-coli
#7
Jay L Mellies, Amy Platenkamp, Jossef Osborn, Lily Ben-Avi
Enteropathogenic Escherichia coli is an important cause of profuse, watery diarrhea in infants living in developing regions of the world. Typical strains of EPEC (tEPEC) possess a virulence plasmid, while related clinical isolates that lack the pEAF plasmid are termed atypical EPEC (aEPEC). tEPEC and aEPEC tend to cause acute vs. more chronic type infections, respectively. The pEAF plasmid encodes an attachment factor as well as a regulatory operon, perABC. PerC, a poorly understood regulator, was previously shown to regulate expression of the type III secretion system through Ler...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28223978/function-and-regulation-of-the-c4-dicarboxylate-transporters-in-campylobacter-jejuni
#8
Marc M S M Wösten, Chris H A van de Lest, Linda van Dijk, Jos P M van Putten
C4-dicarboxylates are important molecules for the human pathogen C.jejuni, as they are used as carbon and electron acceptor molecules, as sugars cannot be utilized by this microaerophilic organism. Based on the genome analysis, C. jejuni may possess five different C4-dicarboxylate transporters: DctA, DcuA, DcuB, and two homologs of DcuC. Here, we investigated the regulation and function of various C4-dicarboxylate transporters in C. jejuni. Transcription of the dctA and dcuC homologs is constitutive, while dcuA and dcuB are both directly regulated by the two-component RacR/RacS system in response to limited oxygen availability and the presence of nitrate...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28223966/geological-and-geochemical-controls-on-subsurface-microbial-life-in-the-samail-ophiolite-oman
#9
Kaitlin R Rempfert, Hannah M Miller, Nicolas Bompard, Daniel Nothaft, Juerg M Matter, Peter Kelemen, Noah Fierer, Alexis S Templeton
Microbial abundance and diversity in deep subsurface environments is dependent upon the availability of energy and carbon. However, supplies of oxidants and reductants capable of sustaining life within mafic and ultramafic continental aquifers undergoing low-temperature water-rock reaction are relatively unknown. We conducted an extensive analysis of the geochemistry and microbial communities recovered from fluids sampled from boreholes hosted in peridotite and gabbro in the Tayin block of the Samail Ophiolite in the Sultanate of Oman...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28223460/mechanism-of-h2s-oxidation-by-the-dissimilatory-perchlorate-reducing-microorganism-azospira-suillum-ps
#10
Misha G Mehta-Kolte, Dana Loutey, Ouwei Wang, Matthew D Youngblut, Christopher G Hubbard, Kelly M Wetmore, Mark E Conrad, John D Coates
The genetic and biochemical basis of perchlorate-dependent H2S oxidation (PSOX) was investigated in the dissimilatory perchlorate-reducing microorganism (DPRM) Azospira suillum PS (PS). Previously, it was shown that all known DPRMs innately oxidize H2S, producing elemental sulfur (S(o)). Although the process involving PSOX is thermodynamically favorable (ΔG°' = -206 kJ ⋅ mol(-1) H2S), the underlying biochemical and genetic mechanisms are currently unknown. Interestingly, H2S is preferentially utilized over physiological electron donors such as lactate or acetate although no growth benefit is obtained from the metabolism...
February 21, 2017: MBio
https://www.readbyqxmd.com/read/28223216/mir-181c-restrains-nitration-stress-of-endothelial-cells-in-diabetic-db-db-mice-through-inhibiting-the-expression-of-foxo1
#11
Guangwei Yang, Yuanbo Wu, Shandong Ye
Endothelial dysfunction played an important role in the progression of diabetes mellitus (DM). miR-181c has been implicated in many diseases, including DM. However, the molecular mechanisms of miR-181c regulate this process remained poorly understood. Healthy ICR mice were divided into control group (n = 10) and db/db DM group (n = 10). The expression of miR-181c and FoxO1 were both investigated in diabetic db/db mice or high glucose-induced endothelial cells (MAECs and END-D). Here we found that down-regulation of miR-181c and the activation of FoxO1/iNOS were observed in mice and endothelial cells...
February 18, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28222602/strategies-for-corrole-functionalization
#12
Joana F B Barata, M Graça P M S Neves, M Amparo F Faustino, Augusto C Tomé, José A S Cavaleiro
This review covers the functionalization reactions of meso-arylcorroles, both at the inner core, as well as the peripheral positions of the macrocycle. Experimental details for the synthesis of all known metallocorrole types and for the N-alkylation reactions are presented. Key peripheral functionalization reactions such as halogenation, formylation, carboxylation, nitration, sulfonation, and others are discussed in detail, particularly the nucleophilic aromatic substitution and the participation of corroles in cycloaddition reactions as 2π or 4π components (covering Diels-Alder and 1,3-dipolar cycloadditions)...
February 22, 2017: Chemical Reviews
https://www.readbyqxmd.com/read/28220869/nitric-oxide-is-involved-in-the-oxytetracycline-induced-suppression-of-root-growth-through-inhibiting-hydrogen-peroxide-accumulation-in-the-root-meristem
#13
Qing-Xiang Yu, Golam Jalal Ahammed, Yan-Hong Zhou, Kai Shi, Jie Zhou, Yunlong Yu, Jing-Quan Yu, Xiao-Jian Xia
Use of antibiotic-contaminated manure in crop production poses a severe threat to soil and plant health. However, few studies have studied the mechanism by which plant development is affected by antibiotics. Here, we used microscopy, flow cytometry, gene expression analysis and fluorescent dyes to study the effects of oxytetracycline (OTC), a widely used antibiotic in agriculture, on root meristem activity and the accumulation of hydrogen peroxide (H2O2) and nitric oxide (NO) in the root tips of tomato seedlings...
February 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28220788/no3-anions-can-act-as-lewis-acid-in-the-solid-state
#14
Antonio Bauzá, Antonio Frontera, Tiddo J Mooibroek
Identifying electron donating and accepting moieties is crucial to understanding molecular aggregation, which is of pivotal significance to biology. Anions such as NO3(-) are typical electron donors. However, computations predict that the charge distribution of NO3(-) is anisotropic and minimal on nitrogen. Here we show that when the nitrate's charge is sufficiently dampened by resonating over a larger area, a Lewis acidic site emerges on nitrogen that can interact favourably with electron rich partners. Surveys of the Cambridge Structural Database and Protein Data Bank reveal geometric preferences of some oxygen and sulfur containing entities around a nitrate anion that are consistent with this 'π-hole bonding' geometry...
February 21, 2017: Nature Communications
https://www.readbyqxmd.com/read/28220590/biosynthesis-and-mass-spectral-fragmentation-pathways-of-13-c-and-15-n-labeled-cytochalasin-d-produced-by-xylaria-arbuscula
#15
Luciana da Silva Amaral, Taicia Pacheco Fill, Luiz Fernando Arruda Santos, Edson Rodrigues-Filho
The fungus Xylaria arbuscula was isolated as an endophyte from Cupressus lusitanica and has shown to be a prominent producer of cytochalasans, mainly cytochalasin C, D and Q. Cytochalasans comprise an important class of fungal secondary metabolites that have aroused attention due to their uncommon molecular structures and pronounced biological activities. Due to the few published studies on the ESI-MS/MS fragmentation of this important class of secondary metabolites, in the first part of our work we studied the cytochalasin D fragmentation pathways using an ESI-Q-ToF mass spectrometer coupled with liquid chromatography...
February 21, 2017: Journal of Mass Spectrometry: JMS
https://www.readbyqxmd.com/read/28220388/real-world-evidence-for-the-antianginal-efficacy-of-trimetazidine-from%C3%A2-the%C3%A2-russian-observational-choice-2-study
#16
Maria Glezer
INTRODUCTION: The guidelines recommend a beta-blocker or calcium channel blocker as the first-line medication for angina, supplemented by other agents for additional symptoms. One such agent is trimetazidine (TMZ), which has been shown to reduce the frequency of anginal episodes and improve exercise performance without affecting haemodynamic parameters. However, extensive real-world evidence for its efficacy in combination with first-line therapies has been lacking. METHODS: The aim of this large-scale, Russian, multicentre, 6-month, open-label, prospective observational study was to assess the effect of adding TMZ modified release 35 mg bid to background antianginal therapy in the real-world clinical setting...
February 20, 2017: Advances in Therapy
https://www.readbyqxmd.com/read/28219917/isosorbide-dinitrate-with-or-without-hydralazine-does-not-reduce-wave-reflections-left-ventricular-hypertrophy-or-myocardial-fibrosis-in-patients-with-heart-failure-with-preserved-ejection-fraction
#17
Payman Zamani, Scott Akers, Haideliza Soto-Calderon, Melissa Beraun, Maheswara R Koppula, Swapna Varakantam, Deepa Rawat, Prithvi Shiva-Kumar, Philip G Haines, Jesse Chittams, Raymond R Townsend, Walter R Witschey, Patrick Segers, Julio A Chirinos
BACKGROUND: Wave reflections, which are increased in patients with heart failure with preserved ejection fraction, impair diastolic function and promote pathologic myocardial remodeling. Organic nitrates reduce wave reflections acutely, but whether this is sustained chronically or affected by hydralazine coadministration is unknown. METHODS AND RESULTS: We randomized 44 patients with heart failure with preserved ejection fraction in a double-blinded fashion to isosorbide dinitrate (ISDN; n=13), ISDN+hydralazine (ISDN+hydral; n=15), or placebo (n=16) for 6 months...
February 20, 2017: Journal of the American Heart Association
https://www.readbyqxmd.com/read/28219782/characterization-of-phospholipid-nitroxidation-by-lc-ms-in-biomimetic-models-and-in-h9c2-myoblast-using-a-lipidomic-approach
#18
Tânia Melo, Pedro Domingues, Teresa M Ribeiro-Rodrigues, Henrique Girão, Marcela A Segundo, M Rosário M Domingues
Under nitroxidative stress conditions, lipids are prone to be modified by reaction with reactive nitrogen species (RNS) and different modifications were reported to occur in fatty acids. However, in the case of phospholipids (PL) studied under nitroxidative stress conditions, only nitroalkene derivatives of phosphatidylcholine (PC) and phosphatidylethanolamine (PE), were reported when using both in vitro biomimetic conditions and in vivo model system of type 1 diabetes mellitus. Therefore, in order to further explore other nitroxidative modifications of PL, a biomimetic model of nitroxidation combined with liquid chromatography mass spectrometry (MS) and MS/MS approaches were used to characterize the nitrated and nitroxidized derivatives of PCs and PEs...
February 17, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28219380/effects-of-nutritional-conditions-on-growth-and-biochemical-composition-of-tetraselmis-sp
#19
Mouna Dammak, Bilel Hadrich, Ramzi Miladi, Mohamed Barkallah, Faiez Hentati, Ridha Hachicha, Céline Laroche, Philippe Michaud, Imen Fendri, Slim Abdelkafi
BACKGROUND: This study aimed to maximize biomass concentration, biomass productivity and biochemical composition of the marine microalga Tetraselmis sp. METHODS: In the current study, Box-Behnken Design was used to model the effect of NaNO3, NaH2PO4, metals and vitamins in the F/2 medium on the growth, total chlorophylls, carotenoids and starch contents. The total chlorophylls content was quantified by spectrophotometry. The FT-IR spectroscopy was used to estimate the biochemical compositions of Tetraselmis sp...
February 20, 2017: Lipids in Health and Disease
https://www.readbyqxmd.com/read/28219063/elevated-nitrosative-stress-in-children-with-chronic-spontaneous-urticaria
#20
Fatih Dilek, Deniz Ozceker, Emin Ozkaya, Eray Metin Guler, Mebrure Yazici, Zeynep Tamay, Abdurrahim Kocyigit, Nermin Guler
BACKGROUND: Chronic spontaneous urticaria (CSU) is an idiopathic condition that seriously affects quality of life. It is well known that oxidative stress and nitrosative stress (NS) are generally involved in many chronic inflammatory diseases. This study aimed to evaluate the possible role of NS in the pathogenesis of CSU. METHODS: Thirty-two children with CSU and 22 healthy control subjects were enrolled in the study. Demographic and clinical features were defined, and disease activity was quantified using the urticaria activity score (UAS)...
February 21, 2017: International Archives of Allergy and Immunology
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