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Carine de Freitas Souza, Cristine Rampelotto, Bruno Bianchi Loureiro, Fernanda Alves Pereira, Adriane Erbice Bianchini, Carine Dahl Corcini, Antonio Sergio Varela Junior, Tatiana Emanuelli, Leila Picolli da Silva, Sílvio Teixeira da Costa, Kalyne Bertolin, Monique Tomazele Rovani, Paulo Bayard Dias Gonçalves, Berta Maria Heinzmann, Bernardo Baldisserotto
In aquaculture, nutrition and supplemented diets have been shown to affect broodstock reproductive performance. In this study, we investigated the effects of dietary supplementation with Cymbopogon flexuosus essential oil (CFEO) microcapsules on reproductive-related parameters in silver catfish (Rhamdia quelen) male broodfish. Adult male broodstocks were separated into three groups according to the concentrations of supplemented CFEO (0.0 = control; 1.0 or 3.0 mL per kg of diet). After 20 days under experimental conditions, the animals were euthanized and the gonads were harvested for gonadosomatic index, sperm analysis, oxidative stress, and histopathology; testosterone levels were measured in the plasma; gene expression of prl, smtl, pomca, and pomcb was assessed in the pituitary gland by real-time PCR...
May 18, 2018: Fish Physiology and Biochemistry
Daniele Missio, Natália Picolli Folchini, Fabio Gallas Leivas, Cecília Isabel Inês Urquiza Machado Pavin, Hirya Fernandes Pinto, Francielli Weber Santos Cibin, Daniela Dos Santos Brum
The aim of the present study was to evaluate the effects of Percoll volume on recovery rate, sperm quality, and embryo development kinetics in in vitro production of cattle embryos. Straws of conventional and sex-sorted semen were allocated to three different volumes of Percoll: 300 μL of each Percoll gradient (90%, 60%, and 30%), Control; 100 μL of each Percoll gradient, P100; and 200 μL of each Percoll gradient, P200. Sperm quality, fertilization rate, and embryo morpho-kinetic development using time lapse cinematography up to 48 h post-insemination were evaluated...
March 4, 2018: Animal Reproduction Science
Aline Teixeira Marins, Cíntia Côrte Real Rodrigues, Charlene Cavalheiro de Menezes, Jeane de Lima Costa Gomes, Maiara Dorneles Costa, Mauro Eugênio Medina Nunes, Mariela de Souza Vieira, Filipe Fagan Donato, Renato Zanella, Leila Picolli da Silva, Vania Lucia Loro
Atrazine (ATZ) is a herbicide worldwide used. That can cause oxidative damage in non-target organisms, such as fish. Furthermore, the threat of exposure to pesticides together with poor nutrition is hazardous to the normal development of fish, and supplementation of the fish diet with antioxidants compounds is an alternative approach to prevent the hazardous effects of pesticide exposure. Here we aimed to investigate the capacity of diphenyl diselenide (PhSe)2 diet supplementation to improve the antioxidant defense of Cyprinus carpio (carp) exposed to environmental concentrations of ATZ...
April 30, 2018: Ecotoxicology and Environmental Safety
Raul Antonio Sperotto, Artur Teixeira de Araújo Junior, Janete Mariza Adamski, Denise Cargnelutti, Felipe Klein Ricachenevsky, Ben-Hur Neves de Oliveira, Renata Pereira da Cruz, Rinaldo Pires Dos Santos, Leila Picolli da Silva, Janette Palma Fett
Cold-tolerance in rice may be related to increased cellulose deposition in the cell wall, membrane fatty acids unsaturation and differential expression of several newly identified genes. Low temperature exposure during early vegetative stages limits rice plant's growth and development. Most genes previously related to cold tolerance in rice are from the japonica subspecies. To help clarify the mechanisms that regulate cold tolerance in young indica rice plants, comparative transcriptome analysis of 6 h cold-treated (10 °C) leaves from two genotypes, cold-tolerant (CT) and cold-sensitive (CS), was performed...
February 2018: Plant Cell Reports
Fernanda Aline de Moura, Fernanda Teixeira Macagnan, Lucciélli Rodrigues Dos Santos, Marilia Bizzani, Carmen Lúcia de Oliveira Petkowicz, Leila Picolli da Silva
The composition and fine structure of pectins found in plant cell walls are heterogeneous, with striking differences, depending on their source, and this eventually determines their functional and technological properties. The aim of this study was to extract and determine the chemical composition and physicochemical properties of pectins from different sources: passion fruit peel, orange pomace, and soy hull. Pectin extraction was performed with heated hydrochloric acid solution, followed by precipitation with 96% ethanol...
September 2017: Journal of Food Science and Technology
Thais Dal Picolli, Kaliane Regalin Aver, Roselei Claudete Fontana, Marli Camassola
Biological pre-treatment seems to be promising being an eco-friendly process, with no inhibitor generated during the process. The potential for elephant grass pre-treatment with white degradation fungi Pleurotus ostreatus, Agaricus blazei, Lentinula edodes, Pleurotus citrinopileatus, and Pleurotus djamor, in isolated or mixed cultures of these strains, was evaluated. The highest activities of enzymes involved in the degradation of lignocellulosic biomass (laccases, endoglucanases, xylanases, and β-glucosidases) were observed for A...
January 2018: Biotechnology Progress
Naglezi de Menezes Lovatto, Fernanda Rodrigues Goulart, Silvandro Tonetto de Freitas, Patricia Inês Mombach, Bruno Bianch Loureiro, Ana Betine Beutinger Bender, Aline Augusti Boligon, João Radünz Neto, Leila Picolli da Silva
An 8-week feeding trial was conducted to evaluate the effect of replacing fish meal with pumpkin seed meal (PSM) or phosphorylated protein concentrate of pumpkin seed meal (PPCPS) on growth and metabolic responses of silver catfish. Five isonitrogenous and isocaloric diets were formulated. Control diet contained fish meal as the main protein source. The treatment groups contained 25 and 50% of either PSM or PPCPS protein replaced the fishmeal protein. A total of 400 silver catfish, with initial mean weight of 24 ± 0...
December 2015: Fish Physiology and Biochemistry
Rosana Ferrari, Silvana Maria Picolli Pugini, Aldo Ivan Cespedes Arce, Ernane Jose Xavier Costa, Mariza Pires de Melo
Tryptophan is an essential amino acid precursor of neurotransmitter serotonin and triptamine. During its metabolism, indole-3-acetic acid (IAA) is generated; this substance presents both antioxidant and prooxidant effects in different biological systems in addition to hipoglicemic effects. To date, electroencephalography (EEG) has been used to evaluate the temporal effect of several substances in neurotransmission. The goal of this study was to characterize the effect of IAA in the brain by analysing the EEG signal and evaluate the oxidative status by means of biochemical parameters...
August 2014: Cell Biochemistry and Function
C B Vargas, F Picolli, C Dani, A V Padoin, C C Mottin
BACKGROUND: Obesity is a pathologic condition that causes functional incapacity, with reduction of quality of life and life expectancy and an increase in mortality. Bariatric surgery is indicated to alleviate associated comorbidities and increase physical capacity. The objective of this work was to evaluate the functional capacity of patients with morbid obesity before and after (3 months) bariatric surgery. METHODS: This was a cross-sectional study involving 67 patients, where 61 were women and six men, with a mean age of 38 ± 10 years and mean BMI of 50...
October 2013: Obesity Surgery
Marcio Festugato, Celso Picolli Coelho, Gustavo Fiedler, Felipe Pioner Machado, Mauro Cabral Gonçalves, Filipe Rech Bassani, Paulo Henrique Pierezan, Alessandro Bersch Osvaldt, Luiz Rohde
OBJECTIVE: To study the effects of hyperbaric oxygen therapy on tissue lesions in an experimental model of acute pancreatitis induced by pancreatic duct ligation. ANIMALS: Forty-eight adult female Wistar rats were randomized into two groups (n=24): control group and hyperbaric oxygen therapy group. INTERVENTION: The second group was treated with a two-hour daily session of hyperbaric oxygen therapy at 2.5 ATA started 6 hours after pancreatic duct ligation...
2008: JOP: Journal of the Pancreas
Leila Picolli da Silva, Maria de Lourdes Santorio Ciocca, Eliana Badiale Furlong
The precision attributes and use of the enzymatic-gravimetric method of Prosky et al. (1992) (AOAC 985.29) were evaluated using corn (BR 5202 Pampa) and oat (UFRGS 15) samples. The effect of laboratory batches carried out in different days were evaluated in six laboratory batches, using for each material one duplicate for total fiber (FT) determination, one duplicate for insoluble fiber (FI) determination and blank ones for FT and for FI (both in duplicate). In order to characterize repetitive aspects, five other FT and FI determinations added to each sample were evaluated, summing up 11 data...
December 2003: Archivos Latinoamericanos de Nutrición
Liliane Janete Grando, Liliane Soares Yurgel, Denise Cantarelli Machado, Carmen Lúcia Silva, Márcia Menezes, Cláudia Picolli
Oral manifestations related to HIV infection were evaluated in 184 children of both genders, aging 0-13 years. The oral exams were carried out at the Ambulatory of Pediatric AIDS, São Lucas Hospital and at the Clinics Hospital, both in Porto Alegre, RS, Brazil, and at the University Hospital and School of Dentistry, State University of New York, Stony Brook, NY, USA, from January 1999 to May 2000. A high prevalence of oral manifestations was observed both in Brazilian (72.73%) and American (53.66%) children...
January 2002: Pesquisa Odontológica Brasileira, Brazilian Oral Research
M de C Ramos, M C Vilares, M Jacques De Moraes, A L Calusni, G N Roscani, E Picolli Alves
The main species of mycobacteria isolated in 51 patients with acquired immune deficiency syndrome (AIDS) admitted to the Clinical Hospital of UNICAMP (Teaching Hospital) in 1996, were studied retrospectively by recording the isolation site and signs of pathogenesis. Of these isolates, 31 (55%) were M.tuberculosis and 20 (45%) M.avium complex. Strains of M.tuberculosis isolated in 1996, 1997, and 1998, originating from 42 patients who had AIDS, were tested for susceptibility to isoniazid and rifampicin. Of these, 34 (81%) were susceptible to both drugs tested, 4 (10%) were resistant to isoniazid and susceptible to rifampicin, 2 (5%) were susceptible to isoniazid and resistant to rifampicin, and 2 (5%) were resistant to both rifampicin and isoniazid...
April 2000: Brazilian Journal of Infectious Diseases
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