Siyu Chen, Keyuan Pu, Yue Wang, Yang Su, Jiamin Qiu, Xin Wang, Kunbin Guo, Jun Hu, Huiwen Wei, Hongbiao Wang, Xiaolong Wei, Yuping Chen, Wen Lin, Wenxiu Ni, Yan Lin, Jiayang Chen, Samuel Kin-Man Lai, Kwan-Ming Ng
High-throughput biofluid metabolomics analysis for screening life-threatening diseases is urgently needed. However, the high salt content of biofluid samples, which introduces severe interference, can greatly limit the analysis throughput. Here, a new 3-D interconnected hierarchical superstructure, namely a "plasmonic gold-on-silica (Au/SiO2 ) double-layered aerogel", integrating distinctive features of an upper plasmonic gold aerogel with a lower inert silica aerogel was successfully developed to achieve in situ separation and storage of inorganic salts in the silica aerogel, parallel enrichment of metabolites on the surface of the functionalized gold aerogel, and direct desorption/ionization of enriched metabolites by the photo-excited gold aerogel for rapid, sensitive, and comprehensive metabolomics analysis of human serum/urine samples...
April 11, 2024: Nanoscale