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https://www.readbyqxmd.com/read/29165728/cardioprotective-effect-of-hydroxysafflor-yellow-a-via-the-cardiac-permeability-transition-pore
#1
Gavin A Huber, Sydney M Priest, Timothy P Geisbuhler
Myocardial ischemia damages cardiac myocytes in part via opening of the mitochondrial permeability transition pore. Preventing this pore's opening is therefore a useful therapeutic goal in treating cardiovascular disease. Hydroxysafflor yellow A has been proposed as a nontoxic alternative to other agents that modulate mitochondrial permeability transition pore opening. In this study, we proposed that hydroxysafflor yellow A prevents mitochondrial permeability transition pore formation in anoxic cardiac myocytes, and thus protects the cell from damage seen during reoxygenation of the cardiac myocytes...
November 17, 2017: Planta Medica
https://www.readbyqxmd.com/read/29165554/the-effect-of-1-5%C3%A2-t-cardiac-magnetic-resonance-on-human-circulating-leucocytes
#2
William R Critchley, Anna Reid, Julie Morris, Josephine H Naish, John P Stone, Alexandra L Ball, Triin Major, David Clark, Nick Waldron, Christien Fortune, Jakub Lagan, Gavin A Lewis, Mark Ainslie, Erik B Schelbert, Daniel M Davis, Matthias Schmitt, James E Fildes, Christopher A Miller
Aims: Investigators have proposed that cardiovascular magnetic resonance (CMR) should have restrictions similar to those of ionizing imaging techniques. We aimed to investigate the acute effect of 1.5 T CMR on leucocyte DNA integrity, cell counts, and function in vitro, and in a large cohort of patients in vivo. Methods and results: In vitro study: peripheral blood mononuclear cells (PBMCs) were isolated from healthy volunteers, and histone H2AX phosphorylation (γ-H2AX) expression, leucocyte counts, and functional parameters were quantified using flow cytometry under the following conditions: (i) immediately following PBMC isolation, (ii) after standing on the benchside as a temperature and time control, (iii) after a standard CMR scan...
November 18, 2017: European Heart Journal
https://www.readbyqxmd.com/read/29155786/isolation-and-cultivation-of-adult-rat-cardiomyocytes
#3
Franziska Nippert, Rolf Schreckenberg, Klaus-Dieter Schlüter
In an intact heart, adjacent cells influence adult cardiomyocytes. With the method of isolation and cultivation of adult cardiomyocytes, a precise investigation of the behavior of these cells under specific treatments and environments is possible. This manuscript presents a protocol for successful isolation and cultivation of adult rat ventricular cardiomyocytes (ARVC). The rat is sacrificed by cervical dislocation under deep anesthesia. Then, the heart is extracted and the aorta is uncovered. Subsequently, perfusion on the Langendorff perfusion system with calcium depletion and collagenase treatment is performed...
October 19, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29155779/a-novel-use-of-three-dimensional-high-frequency-ultrasonography-for-early-pregnancy-characterization-in-the-mouse
#4
Mary C Peavey, Corey L Reynolds, Maria M Szwarc, William E Gibbons, Cecilia T Valdes, Francesco J DeMayo, John P Lydon
High-frequency ultrasonography (HFUS) is a common method to non-invasively monitor the real-time development of the human fetus in utero. The mouse is routinely used as an in vivo model to study embryo implantation and pregnancy progression. Unfortunately, such murine studies require pregnancy interruption to enable follow-up phenotypic analysis. To address this issue, we used three-dimensional (3-D) reconstruction of HFUS imaging data for early detection and characterization of murine embryo implantation sites and their individual developmental progression in utero...
October 24, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29153902/robotic-buccal-mucosal-graft-ureteroplasty-for-complex-ureteral-stricture
#5
Sohrab Arora, Logan Campbell, Mouafak Tourojman, Daniel Pucheril, Lamont R Jones, Craig Rogers
OBJECTIVE: To demonstrate robot-assisted ureterolysis and buccal mucosal graft (BMG) ureteroplasty for the management of a complex, long recurrent ureteral stricture developing after ureterolysis, and also to demonstrate the use of near-infrared fluorescence (NIRF) imaging and intraoperative ureteroscopy during this procedure. METHODS: A 58-year-old man with a history of cabergoline treatment and a cardiac catheterization through the left groin presented with left flank pain and hydronephrosis...
December 2017: Urology
https://www.readbyqxmd.com/read/29150492/predictors-of-mortality-in-patients-with-severe-ischemic-cardiomyopathy-undergoing-surgical-mitral-valve-intervention
#6
Kenya Kusunose, Nancy A Obuchowski, Marc Gillinov, Zoran B Popovic, Scott D Flamm, Brian P Griffin, Deborah H Kwon
BACKGROUND: Ischemic mitral regurgitation is associated with substantial risk of death. Although surgical mitral valve intervention (MVi) may improve symptoms, it has not been shown to improve survival. The aim of this study was to identify predictors of mortality in patients with ischemic mitral regurgitation and MVi. METHODS AND RESULTS: We evaluated 117 consecutive patients (age, 65±10 years) with advanced ischemic cardiomyopathy who underwent cardiac magnetic resonance and subsequent MVi between January 1, 2002 and January 1, 2012...
November 17, 2017: Journal of the American Heart Association
https://www.readbyqxmd.com/read/29146241/cardiac-progenitor-cells-activated-by-mitochondrial-delivery-of-resveratrol-enhance-the-survival-of-a-doxorubicin-induced-cardiomyopathy-mouse-model-via-the-mitochondrial-activation-of-a-damaged-myocardium
#7
Jiro Abe, Yuma Yamada, Atsuhito Takeda, Hideyoshi Harashima
It has been reported that transplanting native cells would lack efficiency without producing artificial cell-tissue, due to the exaggerated oxidative stress in doxorubicin-induced cardiomyopathy. We attempted to activate cardiac progenitor cells (CPCs) by delivering resveratrol to mitochondria using a mitochondrial drug delivery system (MITO-Porter system). We first evaluated the viability of H9c2 cells (a cardio myoblast cell line) after doxorubicin treatment, where H9c2 cells were co-cultured with or without the mitochondria activated CPCs (referred to herein as MITO cell)...
November 14, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/29143226/aligned-ovine-diaphragmatic-myoblasts-overexpressing-human-connexin-43-seeded-on-poly-l-lactic-acid-scaffolds-for-potential-use-in-cardiac-regeneration
#8
Carlos Sebastián Giménez, Paola Locatelli, Florencia Montini Ballarin, Alejandro Orlowski, Ricardo A Dewey, Milagros Pena, Gustavo Abel Abraham, Ernesto Alejandro Aiello, María Del Rosario Bauzá, Luis Cuniberti, Fernanda Daniela Olea, Alberto Crottogini
Diaphragmatic myoblasts (DMs) are precursors of type-1 muscle cells displaying high exhaustion threshold on account that they contract and relax 20 times/min over a lifespan, making them potentially useful in cardiac regeneration strategies. Besides, it has been shown that biomaterials for stem cell delivery improve cell retention and viability in the target organ. In the present study, we aimed at developing a novel approach based on the use of poly (L-lactic acid) (PLLA) scaffolds seeded with DMs overexpressing connexin-43 (cx43), a gap junction protein that promotes inter-cell connectivity...
November 15, 2017: Cytotechnology
https://www.readbyqxmd.com/read/29142197/proteotoxicity-in-cardiac-amyloidosis-amyloidogenic-light-chains-affect-the-levels-of-intracellular-proteins-in-human-heart-cells
#9
Esther Imperlini, Massimiliano Gnecchi, Paola Rognoni, Eduard Sabidò, Maria Chiara Ciuffreda, Giovanni Palladini, Guadalupe Espadas, Francesco Mattia Mancuso, Margherita Bozzola, Giuseppe Malpasso, Veronica Valentini, Giuseppina Palladini, Stefania Orrù, Giovanni Ferraro, Paolo Milani, Stefano Perlini, Francesco Salvatore, Giampaolo Merlini, Francesca Lavatelli
AL amyloidosis is characterized by widespread deposition of immunoglobulin light chains (LCs) as amyloid fibrils. Cardiac involvement is frequent and leads to life-threatening cardiomyopathy. Besides the tissue alteration caused by fibrils, clinical and experimental evidence indicates that cardiac damage is also caused by proteotoxicity of prefibrillar amyloidogenic species. As in other amyloidoses, the damage mechanisms at cellular level are complex and largely undefined. We have characterized the molecular changes in primary human cardiac fibroblasts (hCFs) exposed in vitro to soluble amyloidogenic cardiotoxic LCs from AL cardiomyopathy patients...
November 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29130958/propofol-through-upregulating-caveolin-3-attenuates-post-hypoxic-mitochondrial-damage-and-cell-death-in-h9c2-cardiomyocytes-during-hyperglycemia
#10
Fan Deng, Shuang Wang, Liangqing Zhang, Xiang Xie, Shuyun Cai, Haobo Li, Gui-Ling Xie, Hui-Lai Miao, Changmin Yang, Xin Liu, Zhengyuan Xia
BACKGROUND/AIMS: Hearts from diabetic subjects are susceptible to myocardial ischemia reperfusion (I/R) injury. Propofol has been shown to protect against myocardial I/R injury due to its antioxidant properties while the underlying mechanism remained incompletely understood. Thus, this study aimed to determine whether or not propofol could attenuate myocardial I/R injury by attenuating mitochondrial dysfunction/damage through upregulating Caveolin (Cav)-3 under hyperglycemia. METHODS: Cultured rat cardiomyocyte H9C2 cells were subjected to hypoxia/reoxygenation (H/R) in the absence or presence of propofol under high glucose (HG), and cell viability, lactate dehydrogenase (LDH) and mitochondrial viability as well as creatine kinase-MB (CK-MB), cardiac troponin I (cTnI) and intracellular adenosine triphosphate (ATP) content were measured with colorimetric Enzyme-Linked Immunosorbent Assays...
November 9, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29127238/protective-effects-of-the-mechanistic-target-of-rapamycin-against-excess-iron-and-ferroptosis-in-cardiomyocytes
#11
Yuichi Baba, Jason K Higa, Briana K Shimada, Kate M Horiuchi, Tomohiro Suhara, Motoi Kobayashi, Jonathan D Woo, Hiroko Aoyagi, Karra S Marh, Hiroaki Kitaoka, Takashi Matsui
Clinical studies suggest that myocardial iron is a risk factor for left ventricular remodeling in patients after myocardial infarction (MI). Ferroptosis was recently reported as a mechanism of iron-dependent non-apoptotic cell death. However, ferroptosis in the heart is not well understood. The mechanistic target of rapamycin (mTOR) protects the heart against pathological stimuli such as ischemia. To define the role of cardiac mTOR on cell survival in iron-mediated cell death, we examined cardiomyocyte (CM) cell viability under excess iron and ferroptosis conditions...
November 10, 2017: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/29119262/role-of-cardiac-pet-in-clinical-practice
#12
REVIEW
Brian M Salata, Parmanand Singh
Early identification of atherosclerosis and at-risk lesions plays a critical role in reducing the burden of cardiovascular disease. While invasive coronary angiography serves as the gold standard for diagnosing coronary artery disease, non-invasive imaging techniques provide visualization of both anatomical and functional atherosclerotic processes prior to clinical presentation. The development of cardiac positron emission tomography (PET) has greatly enhanced our capability to diagnose and treat patients with early stages of atherosclerosis...
November 9, 2017: Current Treatment Options in Cardiovascular Medicine
https://www.readbyqxmd.com/read/29115599/aconitine-induces-apoptosis-in-h9c2-cardiac-cells-via-mitochondria%C3%A2-mediated-pathway
#13
Xiangting Gao, Xincai Zhang, Jun Hu, Xuehua Xu, Yuanyi Zuo, Yun Wang, Jingfeng Ding, Hongfei Xu, Shaohua Zhu
Aconitine, a diterpenoid alkaloids derived from Aconitum plants, is widely employed to treat various diseases. The aim of the present study was to investigate the apoptotic effect of aconitine in H9c2 cardiac cells. H9c2 cell apoptosis induced by aconitine was detected by a Cell Counting kit‑8 assay, DAPI staining, Annexin V‑FITC/propidium iodide double staining and western blotting. The effects of aconitine on reactive oxygen species levels and mitochondrial membrane potential were confirmed by fluorescence microscopy and flow cytometry...
October 26, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29113887/injectable-silk-fibroin-hydrogels-functionalized-with-microspheres-as-adult-stem-cells-carrier-systems
#14
Matteo Ciocci, Ilaria Cacciotti, Dror Seliktar, Sonia Melino
Hydrogels are good candidate materials for cell delivery scaffolds because they can mimic the physical, chemical, electrical and biological properties of most of the native tissues. In this study, composite biosynthetic hydrogels were produced by combining the bio-functionality of silk fibroin (SF) with the structural versatility of polyethylene-glycol-diacrylated (PEGDa). The formation of a photopolymerizable PEGDa-SF hydrogel (PSFHy) was optimized for 3D-cell culture. Functionalization of the 3D-PSFHy with protein microspheres (MS) was required to increase the porosity and cell-adhesive properties of the material...
November 4, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29101218/discovery-of-novel-small-molecule-inducers-of-heme-oxygenase-1-that-protect-human-ipsc-derived-cardiomyocytes-from-oxidative-stress
#15
R Jason Kirby, Daniela B Divlianska, Kanupriya Whig, Nadezda Bryan, Camilo J Morfa, Ada Koo, Becky L Hood, Kevin H Nguyen, Patrick Maloney, Satayamaheshwar Peddibhotla, E Hampton Sessions, Paul M Hershberger, Layton H Smith, Siobhan Malany
Oxidative injury to cardiomyocytes plays a critical role in cardiac pathogenesis following myocardial infarction. Transplantation of stem cell-derived cardiomyocytes has recently progressed as a novel treatment to repair damaged cardiac tissue but its efficacy has been limited by poor survival of transplanted cells due to oxidative stress in the post-transplantation environment. Identification of small molecules that activate cardioprotective pathways to prevent oxidative damage and increase survival of stem cells post-transplantation is therefore of great interest to improve the efficacy of stem cell therapies...
November 3, 2017: Journal of Pharmacology and Experimental Therapeutics
https://www.readbyqxmd.com/read/29094213/tissue-evacuated-during-joint-replacement-procedure-as-a-source-of-mononuclear-cells
#16
Jakobsons Eriks, Erglis Kristaps, Patetko Liene, Erglis Martins, Rasma Dortane, Beatrise Rupaine, Simona Krapse, Briede Ieva, Valdis Goncars, Muiznieks Indrikis, Erglis Andrejs
BACKGROUND: Different cell populations from bone marrow were used in various clinical trials for cardiac diseases during last decade. Four clinical studies are ongoing in our institution and enroll patients with cardiac diseases, coronary disease, type 2 diabetes, and osteoarthritis. The density gradient is used to separate bone marrow mononuclear cells. Joint replacement procedures were associated with significant loss of tissue. Usually, excess tissue as bone marrow, peripheral blood and fat are removed to clean operation site...
November 1, 2017: European Journal of Orthopaedic Surgery & Traumatology: Orthopédie Traumatologie
https://www.readbyqxmd.com/read/29079985/preserved-cardiac-blood-supply-workload-balance-in-pediatric-patients-after-aortic-arch-repair
#17
Tomoaki Murakami, Atsuhito Takeda
One of the most important problems in patients with aortic coarctation after aortic arch repair is future cardiovascular disease. We have previously reported that the enhancement of aortic pressure wave reflection in patients could be one of the causes of future cardiovascular diseases, because it results in an increase of the left ventricular workload and is disadvantageous for coronary circulation. Seventeen patients who had undergone aortic arch repair without pressure gradient in their aortic arch were enrolled...
October 27, 2017: Pediatric Cardiology
https://www.readbyqxmd.com/read/29076427/engineered-nanoparticles-their-properties-and-putative-applications-for-therapeutic-approaches-utilizing-stem-cells-for-the-repair-of-atherosclerotic-disease
#18
Dimitry A Chistiakov, Alexandra A Melnichenko, Alexander N Orekhov, Yuri V Bobryshev
BACKGROUND: Stem cell therapy was established as a promising approach for regenerative medicine applications such as cardiac repair. However, current stem cell-based therapeutic strategies have serious challenges such as low retention and viability of transplanted stem cells in the injured myocardium. This significantly limits efficiency of stem cell therapy. In addition, poor knowledge about the fate and survival of stem cells after transplantation represents a major reason of conflicting results from recent clinical studies...
October 27, 2017: Current Drug Targets
https://www.readbyqxmd.com/read/29060215/regression-analysis-and-transfer-function-in-estimating-the-parameters-of-central-pulse-waves-from-brachial-pulse-wave
#19
Chai Rui, Li Si-Man, Xu Li-Sheng, Yao Yang, Hao Li-Ling
This study mainly analyzed the parameters such as ascending branch slope (A_slope), dicrotic notch height (Hn), diastolic area (Ad) and systolic area (As) diastolic blood pressure (DBP), systolic blood pressure (SBP), pulse pressure (PP), subendocardial viability ratio (SEVR), waveform parameter (k), stroke volume (SV), cardiac output (CO) and peripheral resistance (RS) of central pulse wave invasively and non-invasively measured. These parameters extracted from the central pulse wave invasively measured were compared with the parameters measured from the brachial pulse waves by a regression model and a transfer function model...
July 2017: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/29060128/acute-effects-of-incremental-exercise-on-central-hemodynamics-in-young-basketball-athletes
#20
Yahui Zhang, Lin Qi, Lisheng Xu, Yang Yao, Wenlong Lv, Chenglin Du
Acute effects of competitive sports on central hemodynamics are controversial. This study was aimed to evaluate acute effects of incremental exercise on central hemodynamics in young basketball athletes during 1-hour recovery. Fifteen young basketball athletes and fifteen controls underwent the acquisition of peripheral and central blood pressure before exercise and at 0, 5, 10, 15, 20, 30, 40, 50, 60 min after incremental exercise. The incremental exercise was performed by Bruce test. Aortic systolic pressure (ASP), sub-endocardial viability ratio and stroke volume were significantly higher in athletes at all time points measured before and after exercise (P<;0...
July 2017: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
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