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Cardiac viability

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https://www.readbyqxmd.com/read/29344827/molecular-imaging-of-cardiac-remodelling-after-myocardial-infarction
#1
REVIEW
Daniel Curley, Begoña Lavin Plaza, Ajay M Shah, René M Botnar
Myocardial infarction and subsequent heart failure is a major health burden associated with significant mortality and morbidity in western societies. The ability of cardiac tissue to recover after myocardial infarction is affected by numerous complex cellular and molecular pathways. Unbalance or failure of these pathways can lead to adverse remodelling of the heart and poor prognosis. Current clinical cardiac imaging modalities assess anatomy, perfusion, function, and viability of the myocardium, yet do not offer any insight into the specific molecular pathways involved in the repair process...
January 17, 2018: Basic Research in Cardiology
https://www.readbyqxmd.com/read/29337695/automated-fabrication-of-photopatterned-gelatin-hydrogels-for-organ-on-chips-applications
#2
Janna C Nawroth, Lisa L Scudder, Ryan T Halvorson, Jason Tresback, John P Ferrier, Sean P Sheehy, Alex Cho, Suraj Kannan, Ilona Sunyovszki, Josue A Goss, Patrick H Campbell, Kevin Kit Parker
Organ-on-chip platforms aim to improve preclinical models for organ-level responses to novel drug compounds. Heart-on-a-chip assays in particular require tissue engineering techniques that rely on labor-intensive photolithographic fabrication or resolution-limited 3D printing of micropatterned substrates, which limits turnover and flexibility of prototyping. We present a rapid and automated method for large scale on-demand micropatterning of gelatin hydrogels for organ-on-chip applications using a novel biocompatible laser-etching approach...
January 16, 2018: Biofabrication
https://www.readbyqxmd.com/read/29336470/tnf-%C3%AE-induces-drp1-mediated-mitochondrial-fragmentation-during-inflammatory-cardiomyocyte-injury
#3
Yue-Liang Shen, Ying-Zhou Shi, Gai-Ge Chen, Lin-Lin Wang, Ming-Zhi Zheng, Hong-Feng Jin, Ying-Ying Chen
Dynamin-related peptide 1 (Drpl)-mediated mitochondrial fission is an important process associated with cardiac dysfunction under different pathological conditions. The aim of the present study was to investigate the expression of Drpl during inflammatory myocardial injury. Sprague‑Dawley rats were treated intraperitoneally with lipopolysaccharides (LPS). Furthermore, cultured H9C2 cardiomyocytes were treated with LPS, interleukin‑6 (IL‑6) and tumor necrosis factor‑α (TNF‑α). Total and mitochondrial proteins were isolated from the heart tissue of rats and from the H9C2 cardiomyocytes...
January 15, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29321822/pursuing-meaningful-end-points-for-stem-cell-therapy-assessment-in-ischemic-cardiac-disease
#4
REVIEW
Maria Dorobantu, Nicoleta-Monica Popa-Fotea, Mihaela Popa, Iulia Rusu, Miruna Mihaela Micheu
Despite optimal interventional and medical therapy, ischemic heart disease is still an important cause of morbidity and mortality worldwide. Although not included in standard of care rehabilitation, stem cell therapy (SCT) could be a solution for prompting cardiac regeneration. Multiple studies have been published from the beginning of SCT until now, but overall no unanimous conclusion could be drawn in part due to the lack of appropriate end-points. In order to appreciate the impact of SCT, multiple markers from different categories should be considered: Structural, biological, functional, physiological, but also major adverse cardiac events or quality of life...
December 26, 2017: World Journal of Stem Cells
https://www.readbyqxmd.com/read/29309377/the-effects-of-short-term-subnormothermic-perfusion-after-cold-preservation-on-liver-grafts-from-donors-after-cardiac-death-an-ex-vivo-rat-model
#5
Yuta Kakizaki, Shigehito Miyagi, Kenji Shimizu, Koji Miyazawa, Wataru Nakanishi, Kazuaki Tokodai, Yasuyuki Hara, Chikashi Nakanishi, Michiaki Unno, Takashi Kamei, Masafumi Goto, Susumu Satomi
BACKGROUND: We previously reported that short oxygenated warm perfusion before cold storage (CS) had improved the graft viability of rat livers from donors after cardiac death (DCD). In this study, we investigated the effectiveness of short-term oxygenated subnormothermic perfusion for different durations after CS in a rat DCD model. METHODS: We used an isolated perfused rat liver system. In Study 1: the grafts were retrieved from Wistar rats 30min after cardiac arrest (thoracotomy), preserved in CS for 6h, and perfused with oxygenated subnormothermic (20-25°C) Krebs-Henseleit buffer for different durations (0, 15, 30, 60, and 90min groups; n=5 in each)...
January 5, 2018: Transplantation
https://www.readbyqxmd.com/read/29301117/cooperative-effects-of-q10-vitamin-d3-and-l-arginine-on-cardiac-and-endothelial-cells
#6
Claudio Molinari, Vera Morsanuto, Simona Polli, Francesca Uberti
This work demonstrates the cooperative effect of Q10, vitamin D3, and L-arginine on both cardiac and endothelial cells. The effects of Q10, L-arginine, and vitamin D3 alone or combined on cell viability, nitric oxide, and reactive oxygen species productions in endothelial and cardiac cells were studied. Moreover, the involvement of PI3K/Akt and ERK/MAPK pathways leading to eNOS activation as well as the involvement of vitamin D receptor were also investigated. The same agents were tested in an animal model to verify vasodilation, nitric oxide, and reactive oxygen species production...
January 5, 2018: Journal of Vascular Research
https://www.readbyqxmd.com/read/29288528/overexpression-of-mir-22-attenuates-oxidative-stress-injury-in-diabetic-cardiomyopathy-via-sirt-1
#7
Qinghui Tang, Qiang Len, Zheng Liu, W D Wang
BACKGROUND/AIMS: Oxidative stress injury is believed to be important in diabetic cardiomyopathy. Recent evidence indicates that miR-22 plays an important role in various cardiovascular diseases, but the protective role of miR-22 in diabetic cardiomyopathy remains undetermined. METHODS: Diabetes was induced in male C57BL/6 mice by intraperitoneal injection with streptozotocin combined with a high-fat diet and miR-22 was overexpressed following transfection with adeno-associated virus...
December 29, 2017: Cardiovascular Therapeutics
https://www.readbyqxmd.com/read/29288317/aortic-reservoir-function-has-a-strong-impact-on-the-cardiac-blood-supply-workload-balance-in-children
#8
Tomoaki Murakami, Atsuhito Takeda
It has been reported that more than half of the ejected blood from the left ventricle is stored in the aorta during systole and expelled during diastole. One important organ that receives blood flow mainly during diastole is the heart. It is also reported that the cardiac blood supply-workload balance in small children is disadvantageous to the heart. Therefore, we measured the aortic reservoir function and examined the relationship between the aortic reservoir function and the cardiac blood supply-workload balance...
December 29, 2017: Pediatric Cardiology
https://www.readbyqxmd.com/read/29286090/therapeutic-inhibition-of-galectin%C3%A2-3-improves-cardiomyocyte-apoptosis-and-survival-during-heart-failure
#9
Xia Li, Xuan Tang, Jinping Lu, Sheng Yuan
Galectin-3 is an important mediator of cardiac fibrosis and heart failure. Cell viability of cardiomyocytes was measured using a CCK‑8 assay; flow cytometry was employed for the detection of the cell cycle and cardiomyocytes apoptosis. Reverse transcription‑quantitative polymerase chain reaction and western blotting was performed to examine the expression of associated genes and proteins. The present study demonstrated that overexpression of galectin‑3 significantly decreased the viability of cardiomyocytes in a time‑dependent manner, with simultaneous arrest of the cell cycle and induction of apoptosis...
December 20, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29285123/an-in-vitro-endothelial-cell-protective-effect-of-secretory-leukocyte-protease-inhibitor-against-simulated-ischaemia-reperfusion-injury
#10
Nitirut Nernpermpisooth, Eakkapote Prompunt, Sarawut Kumphune
Endothelial dysfunction is an essential deleterious modulator of ischaemia/reperfusion (I/R) injury. Secretory leukocyte protease inhibitor (SLPI) has demonstrated myocardial protection in cardiac transplantation; however, the effect of SLPI in endothelial I/R injury remains unexplored. In the present study, the effect of recombinant human SLPI (rhSLPI) treatment against endothelial cells (ECs) subjected to simulated I/R injury and the effect of treatment at different time points were determined. Human umbilical vein ECs (HUVECs) were subjected to normoxic or simulated I/R (sI/R) conditions, and rhSLPI at concentrations of 1, 10, 100 and 1,000 ng/ml was added to the cells prior to ischaemia, during ischaemia or at the onset of reperfusion...
December 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29283343/cardiac-lesion-mapping-in-vivo-using-intracardiac-myocardial-elastography
#11
Ethan Bunting, Clement Papadacci, Elaine Wan, Vincent Sayseng, Julien Grondin, Elisa E Konofagou
Radio frequency (RF) ablation of the myocardium is used to treat various cardiac arrhythmias. The size, spacing, and transmurality of lesions have been shown to affect the success of the ablation procedure; however, there is currently no method to directly image the size and formation of ablation lesions in real time. Intracardiac myocardial elastography (ME) has been previously used to image the decrease in cardiac strain during systole in the ablated region as a result of the lesion formation. However, the feasibility of imaging multiple lesions and identifying the presence of gaps between lesions has not yet been investigated...
January 2018: IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control
https://www.readbyqxmd.com/read/29283315/comparison-of-central-hemodynamic-parameters-for-young-basketball-athletes-and-control-group
#12
Yahui Zhang, Xiaobing Fan, Lin Qi, Lisheng Xu, Chenglin Du
BACKGROUND: Long-term exercise training may have negative effects on cardiovascular functions. Measurement and calculation of central hemodynamic parameters can comprehensively evaluate the cardiovascular functions. This study aims to compare the central hemodynamics between young basketball athletes and matched controls. METHODS: Total 19 young long-term trained male basketball athletes and 17 matched male recreationally active controls participated. The central hemodynamic parameters such as central blood pressure, pulse pressure, heart rate (HR), augmentation index normalised to 75 bpm (AIx@HR75), augmentation index (AIx), ejection duration (ED), sub-endocardial viability ratio (SEVR) were measured, and total peripheral resistance (TPR), stroke volume (SV) and cardiac output (CO) were calculated...
December 28, 2017: Acta Cardiologica
https://www.readbyqxmd.com/read/29279520/taurine-protects-primary-neonatal-cardiomyocytes-against-apoptosis-induced-by-hydrogen-peroxide
#13
Junnan Wang, Chao Qi, Lulu Liu, Lijing Zhao, Wenzhang Cui, Yuantong Tian, Bin Liu, Jing Li
The aim of this study was to investigate the effects of taurine (Tau) on primary cultured neonatal myocardial cells treated with hydrogen peroxide (H2O2) and the underlying mechanism. Primary cardiac myocytes from neonatal Wistar rats were pre-incubated with Tau, and its effects on cell viability and expression of CaM, CaMKII, p53, Bcl-2, and Bax were examined. Tau enhanced the viability of myocardial cells, decreased apoptosis, and alleviated the intracellular calcium overload, especially at dosages of 40 or 80 mM (P < 0...
December 27, 2017: International Heart Journal
https://www.readbyqxmd.com/read/29278748/protective-effect-of-berberine-against-cardiac-ischemia-reperfusion-injury-by-inhibiting-apoptosis-through-the-activation-of-smad7
#14
Weidong Yao, Xin Wang, Kun Xiao
Berberine (BBR) is an isoquinnoline derivative alkaloid extracted from Rhizoma Coptidis that has the potential to protect myocardial tissues from ischemia/reperfusion (I/R) injuries. We attempted to evaluate the effect of BBR on the proliferation and apoptosis of a hypoxia/reoxygenation (H/R) cell model and to reveal the mechanism driving the improving function of BBR myocardial tissues. The H/R cell model was established using H9c2 rat cardiac myoblasts. The cell viability, apoptotic rates, and cell cycle distribution were measured with CCK-8 assay and flow cytometry...
December 23, 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/29273867/a-new-multi-mode-perfusion-system-for-ex-vivo-heart-perfusion-study
#15
Liming Xin, Bryan Gellner, Roberto Vanin Pinto Ribeiro, Giulia Maria Ruggeri, David Banner, Massimiliano Meineri, Vivek Rao, Jean Zu, Mitesh V Badiwala
Ex vivo heart perfusion has been shown to be an effective means of facilitating the resuscitation and assessment of donor hearts for cardiac transplantation. Over the last ten years however, only a few ex vivo perfusion systems have been developed for this application. While results have been promising, a system capable of facilitating multiple perfusion strategies on the same platform has not yet been realized. In this paper, the design, development and testing of a novel and modular ex vivo perfusion system is described...
December 23, 2017: Journal of Medical Systems
https://www.readbyqxmd.com/read/29258676/a-tumor-necrosis-factor-%C3%AE-and-hypoxia-induced-secretome-therapy-for-myocardial-repair
#16
Kaviyanka Selvasandran, Georges Makhoul, Prashant K Jaiswal, Rishi Jurakhan, Li Li, Khalid Ridwan, Renzo Cecere
BACKGROUND: Poor viability and retention of transplanted bone marrow mesenchymal stem cells (BM-MSC) remains an obstacle in promoting healing after myocardial infarction (MI). This study aimed to understand the migratory, angiogenic, and cardioprotective effects induced by tumor necrosis factor (TNF)-α and hypoxia through rat BM-MSC (rBM-MSC) paracrine secretions, collectively referred to as secretome, after MI. METHODS: Secretome from rBM-MSC cultures treated with various combinations of H9c2 cardiomyoblast-conditioned medium, TNF-α, and hypoxia was initially collected...
December 16, 2017: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/29248607/involvement-of-mitogen-activated-kinase-kinase-7-intracellular-signalling-pathway-in-sunitinib-induced-cardiotoxicity
#17
Samantha Louise Cooper, Hardip Sandhu, Afthab Hussain, Christopher Mee, Helen Maddock
The tyrosine kinase inhibitor Sunitinib is used to treat cancer and is linked to severe adverse cardiovascular events. Mitogen activated kinase kinase 7 (MKK7) is involved in the development of cardiac injury and is a component of the c-Jun N-terminal kinase (JNK) signal transduction pathway. Apoptosis signal-regulating kinase 1 (ASK1) is the upstream activator of MKK7 and is specifically inhibited by 2,7-dihydro-2,7-dioxo-3H-naphtho[1,2,3-de]quinoline-1-carboxylic acid ethyl ester (NQDI-1). This study investigates the role of ASK1, MKK7 and JNK during Sunitinib-induced cardiotoxicity...
December 14, 2017: Toxicology
https://www.readbyqxmd.com/read/29243390/aquaporin-4-blockade-improves-survival-of-murine-heart-allografts-subjected-to-prolonged-cold-ischemia
#18
Katayoun Ayasoufi, Naoki Kohei, Michael Nicosia, Ran Fan, George W Farr, Paul R McGuirk, Marc F Pelletier, Robert L Fairchild, Anna Valujskikh
Prolonged cold ischemia storage (CIS) is a leading risk factor for poor transplant outcome. Existing strategies strive to minimize IRI in transplanted organs, yet there is a need for novel approaches to improve outcomes of marginal allografts and expand the pool of donor organs suitable for transplantation. Aquaporins (AQPs) are a family of water channels that facilitate homeostasis, tissue injury and inflammation. We tested whether inhibition of AQP4 improves the survival of fully MHC-mismatched murine cardiac allografts subjected to 8 h CIS...
December 15, 2017: American Journal of Transplantation
https://www.readbyqxmd.com/read/29239459/ischemic-left-ventricle-systolic-dysfunction-an-evidence-based-approach-in-diagnostic-tools-and-therapeutics
#19
Eduardo Gomes Lima, Felipe Pereira Câmara de Carvalho, Jaime Paula Pessoa Linhares Filho, Fabio Grunspun Pitta, Carlos Vicente Serrano
Coronary artery disease (CAD) associated with left ventricular systolic dysfunction is a condition related to poor prognosis. There is a lack of robust evidence in many aspects related to this condition, from definition to treatment. Ischemic cardiomyopathy is a spectrum ranging from stunned myocardium associated with myocardial fibrosis to hibernating myocardium and repetitive episodes of ischemia. In clinical practice, relevance lies in identifying the myocardium that has the ability to recover its contractile reserve after revascularization...
September 2017: Revista da Associação Médica Brasileira
https://www.readbyqxmd.com/read/29234096/transit-and-integration-of-extracellular-mitochondria-in-human-heart-cells
#20
Douglas B Cowan, Rouan Yao, Jerusha K Thedsanamoorthy, David Zurakowski, Pedro J Del Nido, James D McCully
Tissue ischemia adversely affects the function of mitochondria, which results in impairment of oxidative phosphorylation and compromised recovery of the affected organ. The impact of ischemia on mitochondrial function has been extensively studied in the heart because of the morbidity and mortality associated with injury to this organ. As conventional methods to preserve cardiac cell viability and contractile function following ischemia are limited in their efficacy, we developed a unique approach to protect the heart by transplanting respiration-competent mitochondria to the injured region...
December 12, 2017: Scientific Reports
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