keyword
MENU ▼
Read by QxMD icon Read
search

Tetracycline

keyword
https://www.readbyqxmd.com/read/28345912/fluorogenic-templated-reaction-cascades-for-rna-detection
#1
Willem A Velema, Eric T Kool
Nucleic acids detection is essential to the study of biological processes and to diagnosis of pathological states. Although PCR is highly effective in vitro, methods that can function without prior sample preparation, thermal cycling, or enzymes are of interest due to their simplicity. Most current non-PCR detection methods rely on linear signal amplification, which hinders the detection of small amounts of genetic material. To address this limitation, we tested a new strategy for attaining higher-order signal amplification, in which a target sequence templates a chemical ligation and the product of this reaction is in turn detected with a second templated reaction...
March 27, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28345829/high-fluoroquinolone-resistant-strains-of-helicobacter-pylori-in-the-golden-triangle
#2
Ratha Korn Vilaichone, Thawee Ratanachu ek, Pornpen Gamnarai, Phawinee Subsomwong, Tomahisa Uchida, Yoshio Yamaoka, Varocha Mahachai
Background and aims: Helicobacter pylori (H. pylori) infections, associated with fatal GI diseases such as gastric cancer and MALT lymphoma, remain a major health problem in ASEAN countries. The Golden triangle has long been known as one of Asia’s main opium-producing areas. There have been no prior studies of H. pylori infection in this area. The major objectives of this project were therefore to establish prevalence, antibiotic resistance patterns and associated predictive in the Golden triangle. Methods: We recruited dyspeptic patients in Chiang khong and Chiang saen districts, Chiangrai province of Thailand...
February 1, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/28344395/characterization-of-salmonella-gallinarum-from-an-outbreak-in-raigarh-chhattisgarh
#3
Chandrahas Sannat, Anil Patyal, Nidhi Rawat, R C Ghosh, D K Jolhe, R K Shende, S D Hirpurkar, Sanjay Shakya
AIM: The present investigation was conducted to isolate and characterize Salmonella Gallinarum from an outbreak of fowl typhoid in layer birds. MATERIALS AND METHODS: Clinically ill and dead layer birds from an outbreak were investigated. History, clinical signs, and postmortem lesions were suggestive of fowl typhoid. Postmortem samples including heart blood, intestinal contents, pieces of ovary, and liver were collected and processed immediately for bacterial culture, serotyping and antibiotic sensitivity tests...
February 2017: Veterinary World
https://www.readbyqxmd.com/read/28343010/structural-and-dynamics-studies-of-the-tetr-family-protein-cprb-from-streptomyces-coelicolor-in-complex-with-its-biological-operator-sequence
#4
Hussain Bhukya, Asis K Jana, Neelanjana Sengupta, Ruchi Anand
In Streptomycetes, tetracycline repressor family of transcription regulators (TetR-FTRs) controls various biological processes including antibiotic biosynthesis, cellular morphology and innate resistance. Here, we focus on understanding the structural basis of transcription regulation by CprB, a member of TetR-FTRs from S. coelicolor. CprB is implicated as a receptor of γ-butyrolactones, a class of quorum sensing molecules, responsible for initiating secondary metabolic pathways. In order to understand the molecular mechanism of DNA recognition, the X-ray structure of CprB in complex with its biological relevant operator sequence was solved to a resolution of 3...
March 22, 2017: Journal of Structural Biology
https://www.readbyqxmd.com/read/28341023/molecular-characterization-of-invasive-streptococcus-dysgalactiae-subsp-equisimilis-multicenter-study-argentina-2011-2012
#5
Fernando Traverso, Alejandra Blanco, Pilar Villalón, Noelia Beratz, Juan Antonio Sáez Nieto, Horacio Lopardo
Streptococcus dysgalactiae subsp. equisimilis (SDSE) has virulence factors similar to those of Streptococcus pyogenes. Therefore, it causes pharyngitis and severe infections indistinguishable from those caused by the classic pathogen. The objectives of this study were: to know the prevalence of SDSE invasive infections in Argentina, to study the genetic diversity, to determine the presence of virulence genes, to study antibiotic susceptibility and to detect antibiotic resistance genes. Conventional methods of identification were used...
October 2016: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/28340621/antibacterial-and-antibiotic-resistance-modulatory-activities-of-leaves-and-bark-extracts-of-recinodindron-heudelotii-euphorbiaceae-against-multidrug-resistant-gram-negative-bacteria
#6
Aimé Gabriel Fankam, Jules-Roger Kuiate, Victor Kuete
BACKGROUND: Recinodindron heudelotii (Euphorbiaceae) is a plant used in Africa, particularly in Cameroon to treat various ailments including bacterial infections. In this study, we evaluated the extracts of the leaves (RHL) and bark (RHB) of R. heudelotii for their antibacterial and antibiotic resistance modulating activities against 29 Gram-negative bacteria, including multidrug-resistant (MDR) phenotypes. METHODS: The broth micro-dilution assay was used to evaluate the antibacterial activity, and the antibiotic resistance modulating effects of the plant extracts...
March 24, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28340477/environmental-analysis-of-typical-antibiotic-resistant-bacteria-and-args-in-farmland-soil-chronically-fertilized-with-chicken-manure
#7
Xiang Zhao, Jinhua Wang, Lusheng Zhu, Weili Ge, Jun Wang
Antibiotics and the corresponding resistant bacteria and resistance genes (ARGs) are generally considered emerging pollutants. To assess the impacts of tetracycline (TC) and sulfonamide (SA) antibiotics that are eliminated with fecaluria as drug prototypes, farmland soil used to research long-term fertilization with chicken manure was collected at four sites in Shandong Province. In this study, the rates of bacterial drug resistance to the same antibiotic decreased with an increase in the concentration of that antibiotic, and the resistance rates to TCs were lower than those to SAs...
March 21, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28339716/prevalence-antimicrobial-resistance-and-genetic-diversity-of-campylobacter-coli-and-campylobacter-jejuni-in-ecuadorian-broilers-at-slaughter-age
#8
Christian Vinueza-Burgos, Magali Wautier, Delphine Martiny, Marco Cisneros, Inge Van Damme, Lieven De Zutter
Thermotolerant Campylobacter spp. are a major cause of foodborne gastrointestinal infections worldwide. The linkage of human campylobacteriosis and poultry has been widely described. In this study we aimed to investigate the prevalence, antimicrobial resistance and genetic diversity of C. coli and C. jejuni in broilers from Ecuador. Caecal content from 379 randomly selected broiler batches originating from 115 farms were collected from 6 slaughterhouses located in the province of Pichincha during 1 year. Microbiological isolation was performed by direct plating on mCCDA agar...
February 21, 2017: Poultry Science
https://www.readbyqxmd.com/read/28338777/the-chlamydia-suis-genome-exhibits-high-levels-of-diversity-plasticity-and-mobile-antibiotic-resistance-comparative-genomics-of-a-recent-livestock-cohort-shows-influence-of-treatment-regimes
#9
Helena M B Seth-Smith, Sabrina Wanninger, Nathan Bachmann, Hanna Marti, Weihong Qi, Manuela Donati, Antonietta di Francesco, Adam Polkinghorne, Nicole Borel
Chlamydia suis is an endemic pig pathogen, belonging to a fascinating genus of obligate intracellular pathogens. Of particular interest, this is the only chlamydial species to have naturally acquired genes encoding for tetracycline resistance. To date, the distribution and mobility of the Tet-island is not well understood. Our study focused on whole genome sequencing of 29 C. suis isolates from a recent porcine cohort within Switzerland, combined with data from USA tetracycline-resistant isolates. Our findings show that the genome of C...
March 2, 2017: Genome Biology and Evolution
https://www.readbyqxmd.com/read/28338045/abnormal-levels-of-histone-methylation-in-the-retinas-of-diabetic-rats-are-reversed-by-minocycline-treatment
#10
Wenjun Wang, Simone Sidoli, Wenquan Zhang, Qing Wang, Leilei Wang, Ole N Jensen, Lin Guo, Xiaolu Zhao, Ling Zheng
In this study we quantified the alterations of retinal histone post-translational modifications (PTMs) in diabetic rats using a liquid chromatography - tandem mass spectrometry (LC-MS/MS) approach. Some diabetic rats were subsequently treated with minocycline, a tetracycline antibiotic, which has been shown to inhibit the diabetes-induced chronic inflammation in the retinas of rodents. We quantified 266 differentially modified histone peptides, including 48 out of 83 methylation marks with significantly different abundancein retinas of diabetic rats as compared to non-diabetic controls...
March 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28337503/gut-microbiome-related-metabolic-changes-in-plasma-of-antibiotic-treated-rats
#11
C Behr, H Kamp, E Fabian, G Krennrich, W Mellert, E Peter, V Strauss, T Walk, I M C M Rietjens, B van Ravenzwaay
The intestinal microbiota contributes to the metabolism of its host. Adequate identification of the microbiota's impact on the host plasma metabolites is lacking. As antibiotics have a profound effect on the microbial composition and hence on the mammalian-microbiota co-metabolism, we studied the effects of antibiotics on the "functionality of the microbiome"-defined as the production of metabolites absorbed by the host. This metabolomics study presents insights into the mammalian-microbiome co-metabolism of endogenous metabolites...
March 23, 2017: Archives of Toxicology
https://www.readbyqxmd.com/read/28336494/minocycline-reduces-inflammatory-parameters-in-the-brain-structures-and-serum-and-reverses-memory-impairment-caused-by-the-administration-of-amyloid-%C3%AE-1-42-in-mice
#12
Michelle Lima Garcez, Francielle Mina, Tatiani Bellettini-Santos, Franciellen Gonçalves Carneiro, Aline Pereira da Luz, Gustavo Luis Schiavo, Matheus Scopel Andrighetti, Maylton Grégori Scheid, Renan Pereira Bolfe, Josiane Budni
Alzheimer's disease (AD) is a neurodegenerative disorder and the most common type of age-related dementia. Cognitive decline, beta-amyloid (Aβ) accumulation, neurofibrillary tangles, and neuroinflammation are the main pathophysiological characteristics of AD. Minocycline is a tetracycline derivative with anti-inflammatory properties that has a neuroprotective effect. The aim of this study was to evaluate the effect of minocycline on memory, neurotrophins and neuroinflammation in an animal model of AD induced by the administration of Aβ (1-42) oligomer...
March 20, 2017: Progress in Neuro-psychopharmacology & Biological Psychiatry
https://www.readbyqxmd.com/read/28333355/wgs-to-predict-antibiotic-mics-for-neisseria-gonorrhoeae
#13
David W Eyre, Dilrini De Silva, Kevin Cole, Joanna Peters, Michelle J Cole, Yonatan H Grad, Walter Demczuk, Irene Martin, Michael R Mulvey, Derrick W Crook, A Sarah Walker, Tim E A Peto, John Paul
Background: Tracking the spread of antimicrobial-resistant Neisseria gonorrhoeae is a major priority for national surveillance programmes. Objectives: We investigate whether WGS and simultaneous analysis of multiple resistance determinants can be used to predict antimicrobial susceptibilities to the level of MICs in N. gonorrhoeae. Methods: WGS was used to identify previously reported potential resistance determinants in 681 N. gonorrhoeae isolates, from England, the USA and Canada, with phenotypes for cefixime, penicillin, azithromycin, ciprofloxacin and tetracycline determined as part of national surveillance programmes...
March 10, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28333283/rch51-a-multiply-antibiotic-resistant-acinetobacter-baumannii-st103ip-isolate-carries-resistance-genes-in-three-plasmids-including-a-novel-potentially-conjugative-plasmid-carrying-oxa235-in-transposon-tn6252
#14
Mohammad Hamidian, Steven J Nigro, Rebecca M Hartstein, Ruth M Hall
Objectives: To determine the identity and context of genes conferring antibiotic resistance in a sporadic multiply antibiotic-resistant Acinetobacter baumannii recovered at Royal Children's Hospital, Brisbane. Methods: The antibiotic resistance phenotype for 23 antibiotics was determined using disc diffusion or MIC determination. The whole-genome sequence of RCH51 was determined using the Illumina HiSeq platform. Antibiotic resistance determinants were identified using ResFinder...
March 13, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28333250/the-complexity-of-minocycline-serum-protein-binding
#15
Jian Zhou, Brian T Tran, Vincent H Tam
Objectives: Serum protein binding is critical for understanding the pharmacology of antimicrobial agents. Tigecycline and eravacycline were previously reported to have atypical non-linear protein binding; the percentage of free fraction decreased with increasing total concentration. In this study, we extended the investigation to other tetracyclines and examined the factors that might impact protein binding. Methods: Different minocycline concentrations (0.5-50 mg/L) and perfusion media (saline, 0...
February 25, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28333198/a-tetracycline-inducible-integrative-expression-system-for-streptococcus-pneumoniae
#16
Marina Meiers, Anke Laux, Daniela Eichinger, Anne Sexauer, Patrick Marx, Ralph Bertram, Reinhold Brückner
Inducible gene expression systems are very useful to analyze cellular processes. The ability to switch the expression state of genes of interest may even be crucial if essential traits or genetic instability are involved. An integrative plasmid, pTEX2, was designed using the (anhydro)tetracycline-inducible promoter Pxyl/tet from staphylococcal plasmid pRAB11 to control gene expression in Streptococcus pneumoniae. The system was evaluated by expressing genes of the two-component regulatory system ciaRH of S...
February 21, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28333184/analysis-of-blashv-12-carrying-escherichia-coli-clones-and-plasmids-from-human-animal-and-food-sources
#17
Carla Andrea Alonso, Geovana Brenner Michael, Jun Li, Sergio Somalo, Carmen Simón, Yang Wang, Heike Kaspar, Kristina Kadlec, Carmen Torres, Stefan Schwarz
Objectives: This study aimed at characterizing 23 Escherichia coli isolates from various sources and their respective bla SHV-12 -carrying plasmids and sequencing one of these plasmids completely. Methods: Isolates were typed by XbaI-PFGE, MLST and PCR-based phylotyping. Transformed bla SHV-12 -carrying plasmids were examined by replicon typing, S1-nuclease, conjugation, EcoRI-HindIII-BamHI digests and plasmid MLST. Co-located resistance genes and integrons as well as the bla SHV-12 genetic environment were analysed by PCR and sequencing...
February 17, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28333063/occurrence-and-elimination-of-antibiotics-in-three-sewage-treatment-plants-with-different-treatment-technologies-in-urumqi-and-shihezi-xinjiang
#18
Jiang Liu, Jianjiang Lu, Yanbin Tong, Chao Li
Fourteen antibiotics, including five quinolones (QNs), five sulfonamides (SAs), and four tetracyclines (TCs), were selected to investigate their occurrence and elimination in three sewage treatment plants (STPs) by employing different treatment technologies in Urumqi (two STPs) and Shihezi (one STP), China. The STP in Shihezi was chosen as representative to investigate the distribution of antibiotics in a sludge-sewage system. Results showed that the concentrations of most detected antibiotics ranged from tens to hundreds of nanograms per liter in influent samples and under 100 ng L(-1) in effluent samples...
March 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/28333052/pmma-titania-floating-macrospheres-for-the-photocatalytic-remediation-of-agro-pharmaceutical-wastewater
#19
Adam C Hartley, Joseph B Moss, Kyle J Keesling, Nathanael J Moore, Justin D Glover, Joel E Boyd
Antibiotics such as tetracycline are used on a large scale in agriculture, and can become concentrated in wastewater lagoons that are used in conjunction with confined animal feeding operations. Solar-illuminated titanium dioxide can be used to photocatalytically degrade aqueous tetracycline, but its application in a lagoon environment requires that the photocatalyst be supported on a macroscopic support material to prevent loss of the nanoscale photocatalyst into the environment. In this work, titanium dioxide was deposited within a porous poly(methyl methacrylate) film on the surface of floating 7...
March 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/28332827/subcellular-chemical-imaging-of-antibiotics-in-single-bacteria-using-c60-secondary-ion-mass-spectrometry
#20
Hua Tian, David A Six, Thomas Krucker, Jennifer A Leeds, Nicholas Winograd
The inherent difficulty of discovering new and effective antibacterials and the rapid development of resistance particularly in Gram-negative bacteria, illustrates the urgent need for new methods that enable rational drug design. Here we report the development of 3D imaging cluster secondary ion mass spectrometry (SIMS) as a label-free approach to chemically map small molecules in aggregated and single Escherichia coli cells, with ~300 nm spatial resolution and high chemical sensitivity. The feasibility of quantitative analysis was explored, and a non-linear relationship between treatment dose and signal for tetracycline and ampicillin, two clinically-used antibacterials, was observed...
March 23, 2017: Analytical Chemistry
keyword
keyword
18242
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"