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Trypanosoma cruzi

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https://www.readbyqxmd.com/read/29340783/identification-of-bat-trypanosomes-from-minas-gerais-state-brazil-based-on-18s-rdna-and-cathepsin-l-like-targets
#1
Elaine Cristina Bento, César Gómez-Hernández, Lara Rocha Batista, Laís Anversa, André Luiz Pedrosa, Eliane Lages-Silva, Juan David Ramírez, Luis Eduardo Ramirez
Several bat species can be infected by trypanosomes, but there is not much information about which of these parasites infect bats from Triângulo Mineiro and Alto Paranaíba, Minas Gerais state, Brazil, a formerly endemic region for Trypanosoma cruzi, the causative agent of Chagas disease. The aim of this study was to describe, characterize, and identify the presence of trypanosomes in bats. The captured bats (448) belong to four families and to 19 different species. Of those, 37 bats were found to be positive for trypanosomes by microhematocrit, (infection rate 8...
January 16, 2018: Parasitology Research
https://www.readbyqxmd.com/read/29340464/seroprevalence-of-human-trypanosoma-cruzi-infection-in-the-north-of-estado-de-mexico
#2
Saúl González-Guzmán, Sergio Pichardo-Ávila, Eulalia Mimbrera-Rodríguez, José Antonio Crescencio-Trujillo, María de Lourdes Gasca-Leyva, Fernando Martínez-Hernández, Nancy Rivas, Ricardo Alejandre-Aguilar
INTRODUCTION: Chagas disease is a neglected public health problem in Mexico; however, detailed studies to determine the seroprevalence in some states have not been performed. METHODS: A total 1,504 human serum from thirteen communities in Estado de Mexico, were analyzed with three diagnostics techniques. RESULTS: The overall seroprevalence was 9.1%, with high prevalence among people aged 51-60 years, while people aged 0-29 years were seronegative against T...
November 2017: Revista da Sociedade Brasileira de Medicina Tropical
https://www.readbyqxmd.com/read/29340462/rhodnius-stali-new-vector-infected-by-trypanosoma-rangeli-kinetoplastida-trypanosomatidae
#3
Gabriela Vieira de Souza Castro, Mariane Albuquerque Lima Ribeiro, Leandro José Ramos, Jader de Oliveira, João Aristeu da Rosa, Luis Marcelo Aranha Camargo, Dionatas Ulises de Oliveira Meneguetti
INTRODUCTION: Rhodnius stali infection by Trypanosoma rangeli is reported in this study for the first time. METHODS: The triatomines were collected from the campus of the Federal University of Acre in Rio Branco, Acre, Brazil. The identification of T. rangeli was confirmed by multiplex polymerase chain reaction. RESULTS: The examinations of two specimens revealed R. stali infection by the epimastigote forms of T. rangeli. CONCLUSIONS: The encounter of R...
November 2017: Revista da Sociedade Brasileira de Medicina Tropical
https://www.readbyqxmd.com/read/29340461/synanthropic-triatomines-as-potential-vectors-of-trypanosoma-cruzi-in-central-brazil
#4
Thaís Tâmara Castro Minuzzi-Souza, Nadjar Nitz, César Augusto Cuba Cuba, Marcelo Santalucia, Monique Knox, Luciana Hagström, Camilla Bernardes Furtado, Tamires Emanuele Vital, Marcos Takashi Obara, Mariana Machado Hecht, Rodrigo Gurgel-Gonçalves
INTRODUCTION: Chagas disease surveillance requires current knowledge on synanthropic triatomines. We analyzed the occurrence and Trypanosoma cruzi infection rates of triatomine bugs in central Brazil, during 2012-2014. METHODS: Triatomines were collected inside or around houses, and T. cruzi infection was determined by optical microscopy and conventional/quantitative polymerase chain reaction. RESULTS: Of the 2706 triatomines collected, Triatoma sordida was the most frequent species in Goiás State, whereas Panstrongylus megistus predominated in the Federal District...
November 2017: Revista da Sociedade Brasileira de Medicina Tropical
https://www.readbyqxmd.com/read/29337988/l-arginine-supplementation-reduces-mortality-and-improves-disease-outcome-in-mice-infected-with-trypanosoma-cruzi
#5
Sofía Carbajosa, Héctor O Rodríguez-Angulo, Susana Gea, Carlos Chillón-Marinas, Cristina Poveda, María C Maza, Diana Colombet, Manuel Fresno, Núria Gironès
Chagas disease caused by Trypanosoma cruzi is a neglected disease that affects about 7 million people in Latin America, recently emerging on other continents due to migration. As infection in mice is characterized by depletion of plasma L-arginine, the effect on infection outcome was tested in mice with or without L-arginine supplementation and treatment with 1400W, a specific inhibitor of inducible nitric oxide synthase (iNOS). We found that levels of L-arginine and citrulline were reduced in the heart and plasma of infected mice, whereas levels of asymmetric dimethylarginine, an endogenous iNOS inhibitor, were higher...
January 16, 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29336553/catalytic-mechanism-of-cruzain-from-trypanosoma-cruzi-as-determined-from-solvent-kinetic-isotope-effects-of-steady-state-and-pre-steady-state-kinetics
#6
Xiang Zhai, Thomas D Meek
Cruzain, an important drug target for Chagas disease, is a member of Clan CA of the cysteine proteases. Understanding the catalytic mechanism of cruzain is vital to the design of new inhibitors. To this end, we have performed pH-rate profiles for substrates and affinity agents, and have determined solvent kinetic isotope effects in pre-steady-state and steady-state modes using three substrates: Cbz-Phe-Arg-AMC, Cbz-Arg-Arg-AMC and Cbz-Arg-Ala-AMC. The pH-rate profile of kcat/Km for Cbz-Arg-Arg-AMC indicated groups of pK1 = 6...
January 16, 2018: Biochemistry
https://www.readbyqxmd.com/read/29335789/first-record-of-trypanosoma-dionisii-of-the-t-cruzi-clade-from-the-eastern-bent-winged-bat-miniopterus-fuliginosus-in-the-far-east
#7
Eliakunda Mafie, Fatema Hashem Rupa, Ai Takano, Kazuo Suzuki, Ken Maeda, Hiroshi Sato
Chiropteran mammals worldwide harbour trypanosomes (Euglenozoa: Kinetoplastea: Trypanosomatida) of the subgenus 'Schizotrypanum' in the classical sense. Latterly, these trypanosomes have been referred to as members of the 'Trypanosoma cruzi clade' as their phylogenetic relationships, structure and life cycle conform to T. cruzi, parasitising various terrestrial mammals as well as humans in Latin America. Little is known, however, about the trypanosome species in Asian bats. During a survey on Borrelia spp. in the Eastern bent-winged bat (Miniopterus fuliginosus) living in a cave in Wakayama Prefecture, Japan, incidental proliferation of trypanosomes was detected in two of 94 haemocultures...
January 15, 2018: Parasitology Research
https://www.readbyqxmd.com/read/29332591/anti-trypanosoma-cruzi-activity-in-vitro-of-phases-and-isolated-compounds-from-excoecaria-lucida-leaves
#8
Cristiane Franca da Silva, Ania Ochoa Pacheco, Rayane Nogueira Alves, Josean Fechine Tavares, Marcelo Sobral da Silva, Julio Cesar Escalona Arranz
BACKGROUND: Chagas disease is caused by the protozoan parasite Trypanosoma cruzi. This illness is found mainly in 21 Latin American countries and an estimated 8 million people are infected worldwide. The unsatisfactory chemotherapy provokes severe toxicity and resistant strains. Medicinal plants constitute a promising source of new drugs and remedies against all kinds of disorders, mainly infectious diseases arousing interest worldwide. OBJECTIVE: The aim of this study has been the isolation, structural identification and evaluation of the trypanocidal activity of samples present in the Excoecaria lucida Sw...
January 11, 2018: Medicinal Chemistry
https://www.readbyqxmd.com/read/29323468/in-silico-studies-designed-to-select-sesquiterpene-lactones-with-potential-antichagasic-activity-from-an-in-house-asteraceae-database
#9
Chonny Herrera-Acevedo, Luciana Scotti, Marcus Tullius Scotti
Chagas disease is an endemic disease caused by Trypanosoma cruzi, which affects more than eight million people, mostly in the Americas. A search for new treatments is necessary to control and eliminate this disease. Sesquiterpene lactones (SLs) are an interesting group of secondary metabolites characteristic of Asteraceae that have presented a wide range of biological activities. From the ChEMBL database, we selected a diverse set of 4,452, 1,635 and 1,322 structures with tested activity against the three T...
January 11, 2018: ChemMedChem
https://www.readbyqxmd.com/read/29322297/nuclear-distribution-of-the-trypanosoma-cruzi-rna-pol-i-subunit-rpa31-during-growth-and-metacyclogenesis-and-characterization-of-its-nuclear-localization-signal
#10
Israel Canela-Pérez, Imelda López-Villaseñor, Ana María Cevallos, Roberto Hernández
Trypanosoma cruzi is the aetiologic agent of Chagas disease. Our research group studies ribosomal RNA (rRNA) gene transcription and nucleolus dynamics in this species of trypanosomes. RPA31 is an essential subunit of RNA polymerase I (Pol I) whose presence is apparently restricted to trypanosomes. Using fluorescent-tagged versions of this protein (TcRPA31-EGFP), we describe its nuclear distribution during growth and metacyclogenesis. Our findings indicate that TcRPA31-EGFP alters its nuclear presence from concentrated nucleolar localization in exponentially growing epimastigotes to a dispersed granular distribution in the nucleoplasm of stationary epimastigotes and metacyclic trypomastigotes...
January 10, 2018: Parasitology Research
https://www.readbyqxmd.com/read/29320490/the-glutamine-synthetase-of-trypanosoma-cruzi-is-required-for-its-resistance-to-ammonium-accumulation-and-evasion-of-the-parasitophorous-vacuole-during-host-cell-infection
#11
Marcell Crispim, Flávia Silva Damasceno, Agustín Hernández, María Julia Barisón, Ismael Pretto Sauter, Raphael Souza Pavani, Alexandre Santos Moura, Elizabeth Mieko Furusho Pral, Mauro Cortez, Maria Carolina Elias, Ariel Mariano Silber
Trypanosoma cruzi, the etiological agent of Chagas disease, consumes glucose and amino acids depending on the environmental availability of each nutrient during its complex life cycle. For example, amino acids are the major energy and carbon sources in the intracellular stages of the T. cruzi parasite, but their consumption produces an accumulation of NH4+ in the environment, which is toxic. These parasites do not have a functional urea cycle to secrete excess nitrogen as low-toxicity waste. Glutamine synthetase (GS) plays a central role in regulating the carbon/nitrogen balance in the metabolism of most living organisms...
January 10, 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29317702/surveillance-of-vector-borne-pathogens-under-imperfect-detection-lessons-from-chagas-disease-risk-mis-measurement
#12
Thaís Tâmara Castro Minuzzi-Souza, Nadjar Nitz, César Augusto Cuba Cuba, Luciana Hagström, Mariana Machado Hecht, Camila Santana, Marcelle Ribeiro, Tamires Emanuele Vital, Marcelo Santalucia, Monique Knox, Marcos Takashi Obara, Fernando Abad-Franch, Rodrigo Gurgel-Gonçalves
Vector-borne pathogens threaten human health worldwide. Despite their critical role in disease prevention, routine surveillance systems often rely on low-complexity pathogen detection tests of uncertain accuracy. In Chagas disease surveillance, optical microscopy (OM) is routinely used for detecting Trypanosoma cruzi in its vectors. Here, we use replicate T. cruzi detection data and hierarchical site-occupancy models to assess the reliability of OM-based T. cruzi surveillance while explicitly accounting for false-negative and false-positive results...
January 9, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29315305/serological-and-molecular-inquiry-of-chagas-disease-in-an-afro-descendant-settlement-in-mato-grosso-do-sul-state-brazil
#13
Mariana Furquim da Silva Martins, Mariane Barroso Pereira, Juliana de Jesus Guimarães Ferreira, Adriana de Oliveira França, Marlon Cézar Cominetti, Eduardo de Castro Ferreira, Maria Elizabeth Moraes Cavalheiros Dorval, Cláudio Lúcio Rossi, Sílvia de Barros Mazon, Eros Antonio de Almeida, Sandra Cecília Botelho Costa, Gláucia Elisete Barbosa Marcon
Furnas do Dionísio is a Brazilian Afro-descendant settlement in the city of Jaraguari, 21.4 miles from Campo Grande, Mato Grosso do Sul, Brazil. Approximately 96 families live in this quilombola (Maroon) settlement, also known in Brazil as a remnant community of descendants of African slaves. Recent studies found 20% of households were infested by triatomines, 18% of insects captured in the community were infected by Trypanosoma cruzi, and 22.7% of dogs presented T. cruzi antibodies. The low prevalence of Chagas disease observed in humans in Mato Grosso do Sul State is attributed to its arrival via colonist migration and subsequent transplacental transmission...
2018: PloS One
https://www.readbyqxmd.com/read/29313667/design-and-synthesis-of-broad-spectrum-trypanosomatid-selective-inhibitors
#14
Andrew L Fraser, Stefanie K Menzies, Elizabeth F B King, Lindsay B Tulloch, Eoin R Gould, Marija Zacharova, Terry K Smith, Gordon J Florence
Neglected tropical diseases caused by parasitic infections are an ongoing and increasing concern that have a devastating effect on the developing world due to their burden on human and animal health. In this work, we detail the preparation of a focused library of substituted-tetrahydropyran derivatives and their evaluation as selective chemical tools for trypanosomatid inhibition and the follow-on development of photo-affinity probes capable of labeling target protein(s) in vitro. Several of these functionalised compounds maintain low micromolar activity against Trypanosoma brucei, Trypanosoma cruzi, Leishmania major and Leishmania donovani...
January 9, 2018: ACS Infectious Diseases
https://www.readbyqxmd.com/read/29313485/biological-and-molecular-characterization-of-trypanosoma-cruzi-strains-from-four-states-of-brazil
#15
Aline Rimoldi Ribeiro, Luciana Lima, Larissa Aguiar de Almeida, Joana Monteiro, Cláudia Jassica Gonçalves Moreno, Juliana Damieli Nascimento, Renato Freitas de Araújo, Fernanda Mello, Luciamáre Perinetti Alves Martins, Márcia Aparecida Silva Graminha, Marta Maria Geraldes Teixeira, Marcelo Sousa Silva, Mário Steindel, João Aristeu da Rosa
Chagas disease affects between six and seven million people. Its etiological agent, Trypanosoma cruzi, is classified into six discrete typing units (DTUs). The biological study of 11 T. cruzi strains presented here included four parameters: growth kinetics, parasitemia curves, rate of macrophage infection, and serology to evaluate IgM, total IgG, IgG1, IgG2a, and IgG3. Sequencing of small subunit of ribosomal RNA (SSU rRNA)was performed and the T. cruzi strains were classified into three DTUs. When their growth in liver infusion tryptose medium was represented in curves, differences among the strains could be noted...
January 8, 2018: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29313472/diagnosis-of-trypanosoma-cruzi-infection-status-using-saliva-of-infected-subjects
#16
Nuria Cortes-Serra, Maria-Jesus Pinazo, Leonardo De la Torre, Melina Galizzi, Joaquim Gascon, Juan Manuel Bustamante
Chagas disease has the highest prevalence of any parasitic disease in the Americas, affecting 6-7 million people. Conventional diagnosis requires a well-equipped laboratory with experienced personnel. The development of new diagnostic tools that are easy to use and adapted to the reality of affected populations and health systems is still a significant challenge. The main objective of this study was to measure Trypanosoma cruzi infection status using saliva samples of infected subjects. Blood and saliva samples from 20 T...
January 8, 2018: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29309767/time-and-dose-dependenceevaluation-of-nitroheterocyclic-drugs-for-improving-efficacy-following-trypanosoma-cruzi-infection-a-pre-clinical-study
#17
Ana Lia Mazzeti, Lívia de F Diniz, Karolina R Gonçalves, Alvaro F S Nascimento, Pollyanna A F Spósito, Vanessa C F Mosqueira, George L L Machado-Coelho, Isabela Ribeiro, Maria T Bahia
Benznidazole and nifurtimox-treatments regimens currently used in human are supported by very limited experimental data. This study was designed to evaluate the time and dose dependence for efficacy of the most important nitroheterocyclic drugs in use for Chagas disease. In order to evaluate time dependence, Y strain-infected mice received benznidazole for a total of 1, 3, 7, 10, 20, and 40 days. Treatment courses of 3 to 10-day were effective in clearing parasitaemia and suppressing mortality, but parasitological cure was not achieved...
January 5, 2018: Biochemical Pharmacology
https://www.readbyqxmd.com/read/29306999/assessment-of-the-antiprotozoal-activity-of-pulicaria-inuloides-extracts-an-algerian-medicinal-plant-leishmanicidal-bioguided-fractionation
#18
Hamza Fadel, Ines Sifaoui, Atteneri López-Arencibia, María Reyes-Batlle, Soumaya Hajaji, Olfa Chiboub, Ignacio A Jiménez, Isabel L Bazzocchi, Jacob Lorenzo-Morales, Samir Benayache, José E Piñero
The lack of an effective chemotherapy for treatment of protozoan disease urges a wide investigation for active compounds, and plant-derived compounds continue to provide key leads for therapeutic agents. The current study reports the in vitro antiprotozoal evaluation of the Algerian medicinal plant Pulicaria inuloides against Leishmania amazonensis, Trypanosoma cruzi, and Acanthamoeba castellanii str. Neff. All the extracts from the aerial part showed to be present a higher leishmanicidal activity than anti-Acanthamoeba or Trypanosoma...
January 6, 2018: Parasitology Research
https://www.readbyqxmd.com/read/29305891/efficacy-of-essential-oil-of-syzygium-aromaticum-alone-and-in-combination-with-benznidazole-on-murine-oral-infection-with-trypanosoma-cruzi-iv
#19
Gerson Zanusso Junior, Miyoko Massago, Danielle Kian, Max Jean de Ornelas Toledo
Chagas disease (CD), caused by Trypanosoma cruzi, remains a serious public health problem. One of the causes of the high morbidity and mortality in patients is the lack of an effective drug therapy. Thus, the aim of this study was to evaluate the efficacy of the essential oil of Syzygium aromaticum alone and in combination with benznidazole (BZ) in mice orally inoculated with strain of T. cruzi IV obtained from oral CD outbreak occurred in Western Brazilian Amazonia. All the animals inoculated with metacyclic trypomastigote forms (AM14 strain, BZ resistant), derived from the insect Rhodnius robustus, became infected and there was no difference in the mortality rate between the experimental groups...
January 3, 2018: Experimental Parasitology
https://www.readbyqxmd.com/read/29304849/should-i-stay-or-should-i-go-movement-of-adult-triatoma-sordida-within-the-peridomestic-area-of-a-typical-brazilian-cerrado-rural-household
#20
Edson Santos Dantas, Rodrigo Gurgel-Gonçalves, Daniel Antunes Maciel Villela, Fernando Araújo Monteiro, Rafael Maciel-de-Freitas
BACKGROUND: Chagas disease, or American trypanosomiasis, is an important neglected tropical illness caused by the flagellate protozoan Trypanosoma cruzi, which is primarily transmitted to humans by hematophagous insects of the subfamily Triatominae. Although knowledge on triatomine movement capabilities at the micro-geographical scale is of fundamental importance concerning the development of effective vector control strategies, it remains a poorly understood subject. Furthermore, survival rates and size estimates of natural populations are important topics to consider when evaluating transmission intensity...
January 5, 2018: Parasites & Vectors
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