keyword
MENU ▼
Read by QxMD icon Read
search

CPE

keyword
https://www.readbyqxmd.com/read/29786671/mechanisms-of-action-and-cell-death-associated-with-clostridium-perfringens-toxins
#1
REVIEW
Mauricio A Navarro, Bruce A McClane, Francisco A Uzal
Clostridium perfringens uses its large arsenal of protein toxins to produce histotoxic, neurologic and intestinal infections in humans and animals. The major toxins involved in diseases are alpha (CPA), beta (CPB), epsilon (ETX), iota (ITX), enterotoxin (CPE), and necrotic B-like (NetB) toxins. CPA is the main virulence factor involved in gas gangrene in humans, whereas its role in animal diseases is limited and controversial. CPB is responsible for necrotizing enteritis and enterotoxemia, mostly in neonatal individuals of many animal species, including humans...
May 22, 2018: Toxins
https://www.readbyqxmd.com/read/29784076/analgesic-efficacy-and-hematologic-effects-of-robenacoxib-in-mice
#2
Jennifer A Beninson, Jennifer L Lofgren, Patrick A Lester, Madeline M Hileman, Daniel J Berkowitz, Daniel D Myers
NSAID analgesics may confound models that require inflammation to mimic disease development in humans. This effect presents a challenge for veterinary staff and investigators, because surgery is often necessary to create mouse models of disease and NSAID are first-line analgesics used to treat postoperative pain. We evaluated robenacoxib, a NSAID highly selective for cyclooxygenase 2, in a carrageenan paw edema (CPE) assay and surgical model of venous thrombosis (VT). We generated a mouse-specific dose-response curve by using the CPE assay for robenacoxib doses of 3...
May 1, 2018: Journal of the American Association for Laboratory Animal Science: JAALAS
https://www.readbyqxmd.com/read/29782539/comparison-of-two-commercial-carbapenemase-gene-confirmatory-assays-in-multiresistant-enterobacteriaceae-and-acinetobacter-baumannii-complex
#3
Stephan Rösner, Kevin Gehlweiler, Uta Küsters, Mathias Kolbert, Kirsten Hübner, Niels Pfennigwerth, Dietrich Mack
Multidrug-resistant Gram-negative bacilli (MDR-GNB) producing carbapenemases are increasing at an alarming speed. Rapid confirmation of carbapenemase type will be an important diagnostic step in clinical microbiology laboratories not only to reduce the risk of transmissions but also for optimising antibiotic therapy in the future. We compared diagnostic reliability of two commercially available molecular assays (Check-Direct CPE vs. AID line probe assay) for detection and typing of carbapenemase genes in 80 well-characterized isolates of MDR-GNB...
2018: PloS One
https://www.readbyqxmd.com/read/29778045/massively-parallel-sequencing-of-124-snps-included-in-the-precision-id-identity-panel-in-three-east-asian-minority-ethnicities
#4
Jing Liu, Zheng Wang, Guanglin He, Xueying Zhao, Mengge Wang, Tao Luo, Chengtao Li, Yiping Hou
Massively parallel sequencing (MPS) technologies can sequence many targeted regions of multiple samples simultaneously and are gaining great interest in the forensic community. The Precision ID Identity Panel contains 90 autosomal SNPs and 34 upper Y-Clade SNPs, which was designed with small amplicons and optimized for forensic degraded or challenging samples. Here, 184 unrelated individuals from three East Asian minority ethnicities (Tibetan, Uygur and Hui) were analyzed using the Precision ID Identity Panel and the Ion PGM System...
May 15, 2018: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/29777525/preconcentration-and-determination-of-trace-nickel-and-cobalt-in-milk-based-samples-by-ultrasound-assisted-cloud-point-extraction-coupled-with-flame-atomic-absorption-spectrometry
#5
Nuket Kartal Temel, Kadriye Sertakan, Ramazan Gürkan
In this study, ultrasound-assisted cloud point extraction (UA-CPE) method was developed for the determination of Ni(II) and Co(II) in milk-based products. After extraction and preconcentration, the Ni(II) and Co(II) contents of samples were determined by flame atomic absorption spectrometry (FAAS). After their complexation with hydroxy naphthol blue (HNB) in the presence of cationic surfactant, CTAB at pH 4.0, the Ni(II) and Co(II) were taken within the micellar phase of nonionic surfactant, TX-114. The micellar phase containing the analytes were diluted to a volume of 0...
May 18, 2018: Biological Trace Element Research
https://www.readbyqxmd.com/read/29775856/a-simple-ultrasensitive-electrochemical-sensor-for-simultaneous-determination-of-gallic-acid-and-uric-acid-in-human-urine-and-fruit-juices-based-on-zirconia-choline-chloride-gold-nanoparticles-modified-carbon-paste-electrode
#6
Seyed Alireza Shahamirifard, Mehrorang Ghaedi, Zahra Razmi, Shaaker Hajati
The determination of gallic acid (GA) and uric acid (UA) is essential due to their biological properties. Numerous methods have been reported for the analysis of GA and UA in various real samples. However, the development of a simple, rapid and practical sensor still remains a great challenge. Here, a carbon paste electrode (CPE) was modified by nanocomposite containing zirconia nanoparticles (ZrO2 NPs), Choline chloride (ChCl) and gold nanoparticles (AuNPs) to construct ZrO2 -ChCl-AuNPs/CPE as electrochemical sensor for the simultaneous electro-oxidation of GA and UA...
May 8, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29769274/a-comparative-evaluation-of-four-phenotypic-methods-for-detection-of-class-a-and-b-carbapenemase-producing-enterobacteriaceae-in-china
#7
Menglan Zhou, Danchen Wang, Timothy Kudinha, Qiwen Yang, Shuying Yu, Ying-Chun Xu
Objectives: To evaluate the performance of four phenotypic methods in the detection of carbapenemase-producing Enterobacteriaceae (CPE) in China. Methods: We evaluated the performance of four carbapenemase detection methods, the modified Hodge test (MHT), Carba NP test, meropenem hydrolysis assay (MHA) with 1 and 2-hour incubation, and modified carbapenem inactivation method (mCIM) with meropenem, imipenem, and ertapenem, on 342 carbapenem-resistant Enterobacteriaceae (CRE) in China. Polymerase chain reaction was used as the gold standard...
May 16, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29760136/aztreonam-avibactam-combination-restores-susceptibility-of-aztreonam-in-dual-carbapenemase-producing-enterobacteriaceae
#8
Ka Lip Chew, Michelle K L Tay, Bernadette Cheng, Raymond T P Lin, Sophie Octavia, Jeanette W P Teo
Newly approved Food and Drug Administration (FDA) β-lactam-β-lactamase inhibitor (βL-βLI) inhibitor combinations such as ceftazidime-avibactam and meropenem-vaborbactam are potent inhibitors of class A carbapenemase (typified by KPC [ Klebsiella pneumoniae carbapenemase]-like) (1, 2) but are ineffective against carbapenemase-producing Enterobacteriaceae (CPE) producing class B metallo-β-lactamases (MβLs) such as New Delhi metallo-β-lactamase (NDM) (1-4).….
May 14, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29759210/smart-bi-metallic-perovskite-nanofibers-as-selective-and-reusable-sensors-of-nano-level-concentrations-of-non-steroidal-anti-inflammatory-drugs
#9
Mona A Mohamed, Menna M Hasan, Ibrahim H Abdullah, Ahmed M Abdellah, Ali M Yehia, Nashaat Ahmed, Walaa Abbas, Nageh K Allam
A strategy for trace-level carbon-based electrochemical sensors is investigated via exploring the interesting properties of BaNb2 O6 nanofibers (NFs). Utilizing adsorptive stripping square wave voltammetry (ASSWV), an electrochemical sensing platform was developed based on BaNb2 O6 nanofibers-modified carbon paste electrode (CPE) for the sensitive detection of lornoxicam (LOR). Different techniques were used to characterize the fabricated BaNb2 O6 perovskite NFs. The obtained data show the feasibility to electro-oxidize LOR and paracetamol (PAR) on the surface of the fabricated sensor...
August 1, 2018: Talanta
https://www.readbyqxmd.com/read/29758344/the-effects-of-three-absorption-modifying-critical-excipients-on-the-in-vivo-intestinal-absorption-of-six-model-compounds-in-rats-and-dogs
#10
Dahlgren David, Roos Carl, Johansson Pernilla, Tannergren Christer, Lundqvist Anders, Langguth Peter, Sjöblom Markus, Sjögren Erik, Lennernäs Hans
Pharmaceutical excipients that may affect gastrointestinal (GI) drug absorption are called critical pharmaceutical excipients (CPEs), or absorption-modifying excipients (AMEs) if they act by altering the integrity of the intestinal epithelial cell membrane. Some of these excipients increase intestinal permeability, and subsequently the absorption and bioavailability of the drug. This could have implications for both the assessment of bioequivalence and the efficacy of the absorption-enhancing drug delivery system...
May 11, 2018: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/29757232/ultrasonically-modified-amended-cloud-point-extraction-for-simultaneous-pre-concentration-of-neonicotinoid-insecticide-residues
#11
Rawikan Kachangoon, Jitlada Vichapong, Rodjana Burakham, Yanawath Santaladchaiyakit, Supalax Srijaranai
An effective pre-concentration method, namely amended-cloud point extraction (CPE), has been developed for the extraction and pre-concentration of neonicotinoid insecticide residues. The studied analytes including clothianidin, imidacloprid, acetamiprid, thiamethoxam and thiacloprid were chosen as a model compound. The amended-CPE procedure included two cloud point processes. Triton™ X-114 was used to extract neonicotinoid residues into the surfactant-rich phase and then the analytes were transferred into an alkaline solution with the help of ultrasound energy...
May 12, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29753535/ovopel-%C3%A2-and-carp-pituitary-extract-for-induction-of-reproduction-in-colossoma-macropomum-females
#12
Francielle Novaes Souza, Edenilce de Fatima Ferreira Martins, Ruy Alberto Caetano Corrêa Filho, Janessa Sampaio de Abreu, Luana Barbosa Pires, Danilo Pedro Streit, Nelson Mauricio Lopera-Barrero, Jayme Aparecido Povh
The aim of this study was to evaluate the efficiency of the hormonal inducers Ovopel® and carp pituitary extract (CPE) for induction of reproduction in Colossoma macropomum females. The treatments were CPE at the dose of 5.5 mg/kg divided into two applications (10%; and 90% after 12 h) and Ovopel® at doses of 0.2 and 0.4 pellet/kg body weight in a single application. Eight replicates were used in each of the three treatments, totaling 24 experimental units. The females spawned when treated with the 0...
May 4, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29753257/salt-induced-effects-on-natural-and-inverse-dppc-lipid-membranes-molecular-dynamics-simulation
#13
Seyed Mojtaba Rezaei Sani, Mojdeh Akhavan, Seifollah Jalili
Molecular dynamics (MD) simulations of a dipalmitoylphosphatidylcholine (DPPC) bilayer and its neutral inverse-phosphocholine equivalent (DPCPe) were performed to find salt-induced effects on their surface structure and the nature of ion-lipid interactions. We found that the area per lipid is not considerably affected by the inversion, but the deuterium order parameter of carbon atoms in the region of carbonyl carbons changes dramatically. MD simulations indicate that Ca2+ ions can bind to the surface of both DPPC and DPCPe membranes, but K+ ions do not bind to them...
May 3, 2018: Biophysical Chemistry
https://www.readbyqxmd.com/read/29752158/immortalization-of-chicken-embryonic-liver-derived-cell-line-by-stable-expression-of-hmrp18s-2-for-serotype-4-fowl-adenovirus-propagation
#14
Lei Feng, Li Chen, Junwen Yun, Zhixiang Bi, Yinghua Tang, Peipei Wu, Jibo Hou
Inclusion body hepatitis and hydropericardium-hepatitis syndrome caused by serotype 4 fowl adenovirus (FAdV-4) have emerged in China since 2013. FAdV is usually propagated in primary chicken embryonic liver cells or embryo yolk sac. The aim of this work was to develop an immortalized CEL cell line by stable expression of human mitochondrial ribosomal protein 18S-2, named CEL-hMRP18S-2 cells, for the propagation of FAdV-4. The maximum cell density of CEL-hMRP18S-2 cells could reach 2.65 × 106  cells/ml in four-days culture...
May 8, 2018: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/29752082/pvc-membrane-coated-wire-and-carbon-paste-ion-selective-electrodes-for-potentiometric-determination-of-galantamine-hydrobromide-in-physiological-fluids
#15
Fatehy M Abdel-Haleem, Mohamed Saad, Ahmed Barhoum, Mikhael Bechelany, Mahmoud S Rizk
We report on highly-sensitive ion-selective electrodes (ISEs) for potentiometric determining of galantamine hydrobromide (GB) in physiological fluids. Galantamine hydrobromide (GB) was selected for this study due to its previous medical importance for treating Alzheimer's disease. Three different types of ISEs were investigated: PVC membrane electrode (PVCE), carbon-paste electrode (CPE), and coated-wire electrode (CWE). In the construction of these electrodes, galantaminium-reineckate (GR) ion-pair was used as a sensing species for GB in solutions...
August 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/29737834/ph-controlled-liposomes-for-enhanced-cell-penetration-in-tumor-environment
#16
Michela Barattin, Andrea Mattarei, Anna Balasso, Cristina Paradisi, Laura Cantù, Elena Del Favero, Tapani Viitala, Francesca Mastrotto, Paolo Caliceti, Stefano Salmaso
An innovative pH-switchable colloidal system that can be exploited for site-selective anticancer drug delivery has been generated by liposomes decoration with a new novel synthetic non-peptidic oligo-Arginine cell-penetration enhancer (CPE) and a quenching PEGylated counterpart that detaches from the vesicle surface under the acidic conditions of tumors. The CPE module (Arg4-DAG) is formed by four arginine units conjugated to a first generation (G1) 2,2-bis(hydroxymethyl)propionic acid (bis-MPA)/2,2-bis(aminomethyl)propionic acid (bis-AMPA) polyester dendron terminating with 1,2-distearoyl-3-azidopropane for liposome bilayer insertion...
May 8, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29736850/contralateral-parenchymal-enhancement-on-dynamic-contrast-enhanced-mri-reproduces-as-a-biomarker-of-survival-in-er-positive-her2-negative-breast-cancer-patients
#17
Bas H M van der Velden, Elizabeth J Sutton, Luca A Carbonaro, Ruud M Pijnappel, Elizabeth A Morris, Kenneth G A Gilhuijs
OBJECTIVES: To assess whether contralateral parenchymal enhancement reproduces as an independent biomarker for patient survival in an independent patient cohort from a different cancer institution. METHODS: This is a HIPAA-compliant IRB approved retrospective study. Patients with ER-positive/HER2-negative operable invasive ductal carcinoma and preoperative dynamic contrast-enhanced MRI were consecutively included between 2005 and 2009. The parenchyma of the breast contralateral to known cancer was segmented automatically on MRI and contralateral parenchymal enhancement (CPE) was calculated...
May 7, 2018: European Radiology
https://www.readbyqxmd.com/read/29720002/early-prenatal-ultrasound-diagnosis-of-congenital-thoracic-malformations
#18
Lea Bentur, Michal Gur, Mordechai Pollak, Kamal Masarweh, Ido Solt, Moshe Bronshtein
OBJECTIVES: To evaluate and describe the spectrum and rate of congenital thoracic malformations (CTMs) diagnosed by early prenatal sonography (gestational age (GA) less than 16 weeks). METHODS: A retrospective, cross-sectional analysis of prenatal ultrasound screening tests in a community-based clinic. RESULTS: In 2001-2017, 31 261 prenatal ultrasound tests detected 31 CTMs at a gestational age of 15.2 (range, 11.6-16.0) weeks. The most common malformation was congenital pleural effusion (CPE) (15 fetuses, 0...
May 2, 2018: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/29715994/linewidth-tolerant-and-multi-format-carrier-phase-estimation-schemes-for-coherent-optical-m-qam-flexible-transmission-systems
#19
Tao Yang, Chen Shi, Xue Chen, Min Zhang, Yuefeng Ji, Feng Hua, Yufei Chen
Multi-format and linewidth-tolerant carrier phase estimation (CPE) is a vital part of digital signal processing (DSP) units for future elastic optical transmissions to relax the laser linewidth limitation. In this paper, an innovative CPE scheme outperforming existing CPEs in both universality and performance is presented and verified for multiple quadrature amplitude modulation (QAM) formats. Based on the technique of extended QPSK partitioning and quasi-linear approximation, accurate phase estimation is determined by calculating the intersection of two symmetric straight lines with very low complexity...
April 16, 2018: Optics Express
https://www.readbyqxmd.com/read/29706942/oral-administration-of-recombinant-saccharomyces-boulardii-expressing-ovalbumin-cpe-fusion-protein-induces-antibody-response-in-mice
#20
Ghasem Bagherpour, Hosnie Ghasemi, Bahare Zand, Najmeh Zarei, Farzin Roohvand, Esmat M Ardakani, Mohammad Azizi, Vahid Khalaj
Saccharomyces boulardii , a subspecies of Saccharomyces cerevisiae , is a well-known eukaryotic probiotic with many benefits for human health. In the present study, a recombinant strain of S. boulardii was prepared to use as a potential oral vaccine delivery vehicle. In this sense, a ura3 auxotroph strain of S. boulardii CNCM I-745 (known as S. cerevisiae HANSEN CBS 5926, Yomogi® ) was generated using CRISPR/Cas9 methodology. Then a gene construct encoding a highly immunogenic protein, ovalbumin (OVA), was prepared and transformed into the ura3- S...
2018: Frontiers in Microbiology
keyword
keyword
182
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"