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https://www.readbyqxmd.com/read/28810179/utility-of-ultrasound-assisted-cloud-point-extraction-and-spectophotometry-as-a-preconcentration-and-determination-tool-for-the-sensitive-quantification-of-mercury-species-in-fish-samples
#1
Nail Altunay
The current study reports, for the first time, the development of a new analytical method employing ultrasound assisted-cloud point extraction (UA-CPE) for the extraction of CH3Hg(+) and Hg(2+) species from fish samples. Detection and quantification of mercury species were performed at 550nm by spectrophotometry. The analytical variables affecting complex formation and extraction efficiency were extensively evaluated and optimized by univariate method. Due to behave 14-fold more sensitive and selective of thiophene 2,5-dicarboxylic acid (H2TDC) to Hg(2+) ions than CH3Hg(+) in presence of mixed surfactant, Tween 20 and SDS at pH5...
August 10, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28799835/occurrence-of-carbapenemase-producing-enterobacteriaceae-isolates-in-the-wildlife-first-report-of-oxa-48-in-wild-boars-in-algeria
#2
Taous Bachiri, Sofiane Bakour, Rym Lalaoui, Nadia Belkebla, Meriem Allouache, Jean Marc Rolain, Abdelaziz Touati
The aim of the present study was to screen for the presence of carbapenemase-producing Enterobacteriaceae (CPE) isolates from wild boars and Barbary macaques in Algeria. Fecal samples were collected from wild boars (n = 168) and Barbary macaques (n = 212), in Bejaia, Algeria, between September 2014 and April 2016. The isolates were identified and antimicrobial susceptibility was determined. Carbapenem resistance determinants were studied using PCR and sequencing, while clonal relatedness was performed using multilocus sequence typing (MLST)...
August 11, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28792548/electrocatalytic-reduction-of-co2-with-ccc-nhc-pincer-nickel-complexes
#3
James D Cope, Nalaka P Liyanage, Paul J Kelley, Jason A Denny, Edward J Valente, Charles Edwin Webster, Jared H Delcamp, T Keith Hollis
A CCC-NHC pincer Ni(ii)Cl complex was prepared according to the metallation/transmetallation methodology. It was fully characterized by electrochemical, NMR spectroscopic, theoretical, and X-ray crystallographic methods. The complex and its cation were evaluated for electrocatalytic reduction of CO2 under a variety of conditions and found to provide some of the fastest catalytic rates and highest substrate selectivities (CO2vs. H(+)) reported. Rates improved in the presence of water and, significantly, catalysis occurred at the first reduction potential, presumably at the Ni(i) state...
August 9, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28792452/complexes-of-oligoribonucleotides-with-d-mannitol-inhibit-hemagglutinin-glycan-interaction-and-suppress-influenza-a-virus-h1n1-a-fm-1-47-infectivity-in-vitro
#4
Nataliia Melnichuk, Larisa Semernikova, Zenoviy Tkachuk
The influenza virus hemagglutinin (HA) mediates both receptor (glycan) binding and membrane fusion for cell entry and has been the basis for subtyping influenza viruses. The oligoribonucleotides-d-mannitol (ORNs-d-M) complexes possess an anti-influenza activity in vitro and in vivo. In the present studies, we have found that ORNs-d-M interferes with hemagglutinin (HA)-glycan interaction and suppress viral infection in host cells. HA-glycan interactions were evaluated to indirectly quantify the amount of influenza virus titer by an agglutination assay...
August 9, 2017: Pharmaceuticals
https://www.readbyqxmd.com/read/28792450/determination-of-zinc-cadmium-lead-copper-and-silver-using-a-carbon-paste-electrode-and-a-screen-printed-electrode-modified-with-chromium-iii-oxide
#5
Zuzana Koudelkova, Tomas Syrovy, Pavlina Ambrozova, Zdenek Moravec, Lubomir Kubac, David Hynek, Lukas Richtera, Vojtech Adam
In this study, the preparation and electrochemical application of a chromium(III) oxide modified carbon paste electrode (Cr-CPE) and a screen printed electrode (SPE), made from the same material and optimized for the simple, cheap and sensitive simultaneous determination of zinc, cadmium, lead, copper and the detection of silver ions, is described. The limits of detection and quantification were 25 and 80 µg·L(-1) for Zn(II), 3 and 10 µg·L(-1) for Cd(II), 3 and 10 µg·L(-1) for Pb(II), 3 and 10 µg·L(-1) for Cu(II), and 3 and 10 µg·L(-1) for Ag(I), respectively...
August 9, 2017: Sensors
https://www.readbyqxmd.com/read/28790119/complementary-value-of-contralateral-parenchymal-enhancement-on-dce-mri-to-prognostic-models-and-molecular-assays-in-high-risk-er-her2-breast-cancer
#6
Bas H M van der Velden, Sjoerd G Elias, Tycho Bismeijer, Claudette E Loo, Max A Viergever, Lodewyk F A Wessels, Kenneth G A Gilhuijs
Purpose: To determine whether markers of healthy breast stroma are able to select a subgroup of patients at low risk of death or metastasis from patients considered at high risk according to routine markers of the tumor. <p>Experimental Design: Patients with ER-positive/HER2-negative breast cancer were consecutively included for retrospective analysis. The contralateral parenchyma was segmented automatically on dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI), whereupon the average of the top-10% late enhancement was calculated...
August 8, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/28789013/carrier-phase-estimation-algorithm-featuring-fast-trackability-for-high-speed-coherent-wdm-pon-based-on-rsoa
#7
Daeho Kim, Byung Gon Kim, Sung Hyun Bae, Hoon Kim
There have been substantial efforts to implement high-speed (>10 Gb/s) upstream transmission using reflective semiconductor optical amplifiers (RSOAs) in a coherent wavelength-division-multiplexed (WDM) passive optical network (PON). In such a network, it is necessary to estimate the carrier phase of upstream optical signal to retrieve the phase-modulated information created by RSOA. However, due to the severe waveform distortions caused by the limited modulation bandwidth of RSOA (typically less than 3 GHz), previously reported carrier phase estimation (CPE) algorithms cannot accurately estimate the carrier phase of high-speed quadrature phase-shift keying (QPSK) signal generated from the RSOA seeded by a distributed-feedback (DFB) laser...
June 26, 2017: Optics Express
https://www.readbyqxmd.com/read/28782704/geographical-variation-in-therapy-for-bloodstream-infections-due-to-multidrug-resistant-enterobacteriaceae-a-post-hoc-analysis-of-the-increment-study
#8
Patrick N A Harris, M Diletta Pezzani, Belén Gutiérrez-Gutiérrez, Pierluigi Viale, Po-Ren Hsueh, Patricia Ruiz-Garbajosa, Mario Venditti, Mario Tumbarello, Carolina Navarro-Francisco, Esther Calbo, Murat Akova, Helen Giamarellou, Antonio Oliver, Benito Almirante, Oriol Gasch, Luis Martínez-Martínez, Mitchell J Schwaber, George Daikos, Johann Pitout, Carmen Peña, Alicia Hernández-Torres, Yohei Doi, Federico Pérez, Felipe Francisco Tuon, Evelina Tacconelli, Yehuda Carmeli, Robert A Bonomo, Álvaro Pascual, David L Paterson, Jesús Rodríguez-Baño
We aimed to describe regional differences in therapy for bloodstream infection (BSI) caused by extended-spectrum β-lactamase-producing Enterobacteriaceae (ESBL-E) or carbapenemase-producing Enterobacteriaceae (CPE). 1,482 patients in 12 countries were included from an observational study of BSI caused by ESBL-E or CPE. Multivariate logistic regression was used to calculate adjusted odds ratios (aORs) for the influence of country of recruitment on empirical use of β-lactam/β-lactamase inhibitors (BLBLI) or carbapenems, targeted use of BLBLI for ESBL-E and use of targeted combination therapy for CPE...
August 3, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28779659/retrospective-use-of-next-generation-sequencing-reveals-the-presence-of-enteroviruses-in-acute-influenza-like-illness-respiratory-samples-collected-in-south-south-east-asia-during-2010-2013
#9
Wiriya Rutvisuttinunt, Chonticha Klungthong, Butsaya Thaisomboonsuk, Piyawan Chinnawirotpisan, Chuanpis Ajariyakhajorn, Wudtichai Manasatienkij, Thipwipha Phonpakobsin, Chanthap Lon, David Saunders, Sonam Wangchuk, Sanjaya K Shrestha, John Mark S Velasco, Maria Theresa P Alera, Sriluck Simasathien, Darunee Buddhari, Richard G Jarman, Louis R Macareo, In-Kyu Yoon, Stefan Fernandez
BACKGROUND: Emerging and re-emerging respiratory pathogens represent an increasing threat to public health. Etiological determination during outbreaks generally relies on clinical information, occasionally accompanied by traditional laboratory molecular or serological testing. Often, this limited testing leads to inconclusive findings. The Armed Forces Research Institute of Medical Sciences (AFRIMS) collected 12,865 nasopharyngeal specimens from acute influenza-like illness (ILI) patients in five countries in South/South East Asia during 2010-2013...
July 14, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28777309/biological-evaluation-of-uridine-derivatives-of-2-deoxy-sugars-as-potential-antiviral-compounds-against-influenza-a-virus
#10
Ewelina Krol, Ilona Wandzik, Martyna Krejmer-Rabalska, Boguslaw Szewczyk
Influenza virus infection is a major cause of morbidity and mortality worldwide. Due to the limited ability of currently available treatments, there is an urgent need for new anti-influenza drugs with broad spectrum protection. We have previously shown that two 2-deoxy sugar derivatives of uridine (designated IW3 and IW7) targeting the glycan processing steps during maturation of viral glycoproteins show good anti-influenza virus activity and may be a promising alternative approach for the development of new anti-influenza therapy...
August 4, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28772235/discovery-of-methylsulfonyl-indazoles-as-potent-and-orally-active-respiratory-syncytial-virus-rsv-fusion-inhibitors
#11
Song Feng, Chao Li, Dongdong Chen, Xiufang Zheng, Hongying Yun, Lu Gao, Hong C Shen
Recently we described a novel class of imidazopyridine compounds that showed exceptional anti-RSV potency in cell culture. However, unfavorable pharmacokinetic (PK) properties and glutathione (GSH) adduct liabilities impeded their further development. In a bid to address the PK and early safety concerns, a small compound library consisting of dozens of scaffold-hopping analogues was designed and synthesized for RSV CPE assay screening, which led to the identification of a new chemical starting point: methylsulfonyl indole compound 8...
July 20, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28760340/synergistic-effects-of-combination-treatment-using-egcg-and-suramin-against-the-chikungunya-virus
#12
Jeng-Wei Lu, Po-Shiuan Hsieh, Chang-Chi Lin, Ming-Kuan Hu, Shih-Ming Huang, Yu-Ming Wang, Chun-Yu Liang, Zhiyuan Gong, Yi-Jung Ho
Chikungunya is a severe disease that results from infection with the chikungunya virus (CHIKV), an arbovirus. Thus, we (1) explored a new approach to combining previously researched drugs that have shown the potential to inhibit CHIKV infection; and (2) demonstrated the antiviral effects of (-)-Epigallocatechin-3-gallate (EGCG) and the underlying mechanisms. Specifically, we used U2OS cells infected with CHIVK to assess the synergistic antiviral activities of EGCG and suramin. EGCG presented the ability to inhibit the viral RNA, progeny yield, and cytopathic effect (CPE) of CHIKV and also demonstrated the ability to protect against virus entry, replication, and release...
July 28, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28758082/a-new-instrument-for-measuring-tibial-torsion-in-pediatric-patients
#13
Ji Hyun Jeon, Yong-Soon Yoon, Kwang Jae Lee, Ki Pi Yu, Jong Hoo Lee, Tae Yong Seog, EunJi Son
OBJECTIVE: To develop and test the validity and reliability of a new instrument for measuring the thigh-foot angle (TFA) for the patients with in-toeing and out-toeing gait. METHODS: The new instrument (Thigh-Foot Supporter [TFS]) was developed by measuring the TFA during regular examination of the tibial torsional status. The study included 40 children who presented with in-toeing and out-toeing gaits. We took a picture of each case to measure photographic-TFA (P-TFA) in the proper position and to establish a criterion...
June 2017: Annals of Rehabilitation Medicine
https://www.readbyqxmd.com/read/28757020/experimental-infection-of-tilapia-lake-virus-tilv-in-nile-tilapia-oreochromis-niloticus-and-red-tilapia-oreochromis-spp
#14
Puntanat Tattiyapong, Worawan Dachavichitlead, Win Surachetpong
Since 2015, a novel orthomyxo-like virus, tilapia lake virus (TiLV) has been associated with outbreaks of disease and massive mortality of cultured Nile and red tilapia (Oreochromis niloticus and Oreochromis spp., respectively) in Thailand. In this study, TiLV was isolated from field samples and propagated in the permissive E-11 cell line, with cytopathic effect (CPE) development within 3-5days post-inoculation. Electron micrographs of infected E-11 cells and fish tissues confirmed the rounded, enveloped virions of 60 to 80nm with characteristics very similar to those of Orthomyxoviridae...
August 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28755931/blood-plasma-traits-associated-with-genetic-merit-for-feed-utilization-in-holstein-cows
#15
C D Dechow, C R Baumrucker, R M Bruckmaier, J W Blum
The objective of this study was to evaluate the potential of selection for feed utilization on associated blood plasma metabolite and hormone traits. Dry matter intake (DMI) was recorded in 970 Holsteins from 11 commercial farms in Pennsylvania and used to derive dry matter efficiency (DME; fat-corrected milk yield/DMI), crude protein efficiency (CPE; protein yield/crude protein intake), and residual feed intake (RFI, defined as actual feed intake minus expected feed intake for maintenance and milk production, based on calculation of DMI adjusted for yield, body weight, and body condition score)...
July 26, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28754995/genetic-variation-and-population-structure-of-botswana-populations-as-identified-with-ampflstr-identifiler-short-tandem-repeat-str-loci
#16
Tiroyamodimo Tau, Anthony Wally, Thokozile Patricia Fanie, Goitseone Lorato Ngono, Sununguko Wata Mpoloka, Sean Davison, María Eugenia D'Amato
Population structure was investigated in 990 Botswana individuals according to ethno-linguistics, Bantu and Khoisan, and geography (the nine administrative districts) using the Identifiler autosomal microsatellite markers. Genetic diversity and forensic parameters were calculated for the overall population, and according to ethno-linguistics and geography. The overall combined power of exclusion (CPE) was 0.9999965412 and the combined match probability 6,28 × 10(-19). CPE was highest for the Khoisan Tuu ethnolinguistic group and the Northeast District at 0...
July 28, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28754498/a-pragmatic-introduction-of-mindfulness-in-a-continuing-education-setting-exploring-personal-experience-bridging-to-professional-practice
#17
Maryanna D Klatt, Kellie Weinhold, Christopher A Taylor, Kayla Zuber, Cynthia J Sieck
CONTEXT: Continuing Medical Education and Continuing Professional Education (CME/CPE) provide a context through which to introduce practicing Healthcare Professionals (HCPs) to emerging mind/body approaches. OBJECTIVE: To introduce mindfulness to practicing HCPs for application in their practice through an experiential CME/CPE session. DESIGN: This descriptive study included surveys administered in the context of a CME/CPE session at professional meetings, as well as a three-month follow-up survey...
June 30, 2017: Explore: the Journal of Science and Healing
https://www.readbyqxmd.com/read/28736948/evaluating-the-efficacy-of-hydrogen-peroxide-vapour-against-foot-mouth-disease-virus-within-a-bsl4-biosafety-facility
#18
B M Petit, F C Almeida, T R Uchiyama, F O C Lopes, K H Tino, J Chewins
An evaluation was made of the efficacy of 35% Hydrogen Peroxide Vapour (HPV) against FMDV in a biosafety facility. Biological indicators (BIs) were produced using three serotypes of FMDV, all with a titre of ≥10(6) TCID50 /ml. Fifteen BIs of each serotype were distributed across 5 locations, throughout a 30m(3) Air Lock chamber, producing a total of 45 BIs. 35% HPV was generated and applied using a Bioquell vaporisation module located in the centre of the chamber. After a dwell period of 40 minutes, the HPV was removed via the enclosures air handling system and the BIs were collected...
July 23, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28736077/replication-of-the-zika-virus-in-different-ipsc-derived-neuronal-cells-and-implications-to-assess-efficacy-of-antivirals
#19
Kristina Lanko, Kristel Eggermont, Abdulsamie Patel, Suzanne Kaptein, Leen Delang, Catherine M Verfaillie, Johan Neyts
Infections with the Zika virus (ZIKV) are responsible for congenital abnormalities and neurological disorders. We here demonstrate that ZIKV productively infects three types of human iPSC (induced pluripotent stem cells)-derived cells from the neural lineage, i.e. cortical and motor neurons as well as astrocytes. ZIKV infection results in all three cell types in the production of infectious virus particles and induces cytopathic effects (CPE). In cortical and motor neurons, an Asian isolate (PRVABC59) produced roughly 10-fold more virus than the prototypic African strain (MR766 strain)...
July 20, 2017: Antiviral Research
https://www.readbyqxmd.com/read/28734615/highly-cross-linked-polyethylene-reduces-osteolysis-incidence-and%C3%A2-wear-related-reoperation-rate-in-cementless-total-hip-arthroplasty%C3%A2-compared-with-conventional-polyethylene-at%C3%A2-a%C3%A2-mean%C3%A2-12-year-follow-up
#20
Manabu Tsukamoto, Toshiharu Mori, Hideo Ohnishi, Soshi Uchida, Akinori Sakai
BACKGROUND: A number of studies on total hip arthroplasty have compared highly cross-linked polyethylene (HXLPE) with conventional polyethylene (CPE) liners beyond 10 years. However, the impact of HXLPE on the wear-related reoperation rate is unclear. The purpose of this study was to evaluate the clinical advantage of using a single manufacturer's HXLPE in terms of reducing the reoperation rate. METHODS: The study was a follow-up retrospective cohort study over a mean of 12 years that examined patients aged 45-70 years with cementless total hip arthroplasty using a 26-mm-diameter cobalt-chromium head...
July 8, 2017: Journal of Arthroplasty
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