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https://www.readbyqxmd.com/read/29351668/evaluation-of-the-rapid-carbapenem-inactivation-method-rcim-a-phenotypic-screening-test-for-carbapenemase-producing-enterobacteriaceae
#1
Madalina-Maria Muntean, Andrei-Alexandru Muntean, Lauraine Gauthier, Elodie Creton, Garance Cotellon, Mircea Ioan Popa, Rémy A Bonnin, Thierry Naas
Objectives: Fast and accurate diagnostic tests to identify carbapenemase-producing Enterobacteriaceae (CPE) are mandatory for proper antimicrobial therapy and implementing infection control measures. Here, we have developed a rapid Carbapenem Inactivation Method (rCIM) for CPE detection. Methods: The rCIM consists of the incubation of a potential carbapenemase producer with meropenem discs and use of the resulting supernatant to challenge a susceptible indicator strain...
January 16, 2018: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29344099/audit-of-the-management-of-patients-at-high-risk-of-carbapenemase-producing-enterobacteriaceae-cpe-are-we-ready
#2
Lasantha Ratnayake, Amy Harris, Doreen Ko, Linda Hawtin
Background: Incidence of carbapenemase-producing enterobacteriaceae (CPE) in the UK is increasing. In 2013, Public Health England (PHE) published a toolkit to control spread of CPE within healthcare settings. Aim: To assess compliance to hospital CPE policy (adapted from PHE) in the identification, isolation and screening of suspected CPE patients. Methods: Admission booklets of 150 patients were evaluated to see whether the relevant section had been completed to identify high-risk CPE patients...
November 2017: Journal of Infection Prevention
https://www.readbyqxmd.com/read/29341586/development-of-a-novel-cytopathic-effect-based-phenotypic-screening-assay-against-cryptosporidium
#3
Alexander T Chao, Boon Heng Lee, Kah Fei Wan, Jeremy Selva, Bin Zou, Peter Gedeck, David Beer, Thierry T Diagana, Ghislain Bonamy, Ujjini H Manjunatha
Cryptosporidiosis is a diarrheal disease predominantly caused by Cryptosporidium parvum (Cp) and Cryptosporidium hominis (Ch), apicomplexan parasites which infect the intestinal epithelial cells of their human hosts. The only approved drug for cryptosporidiosis is nitazoxanide, which shows limited efficacy in immunocompromised children, the most vulnerable patient population. Thus, new therapeutics and in vitro infection models are urgently needed to address the current unmet medical need. Toward this aim, we have developed novel cytopathic effect (CPE)-based Cp and Ch assays in human colonic tumor (HCT-8) cells and compared them to traditional imaging formats...
January 17, 2018: ACS Infectious Diseases
https://www.readbyqxmd.com/read/29338176/polystyrene-block-poly-ionic-liquid-copolymers-as-work-function-modifiers-in-inverted-organic-photovoltaic-cells
#4
Jong Baek Park, Mehmet Isik, Hea Jung Park, In Hwan Jung, David Mecerreyes, Do-Hoon Hwang
Interfacial layers play a critical role in building up the ohmic contact between the electrodes and functional layers in organic photovoltaic solar cells. These layers are based on either inorganic oxides (ZnO, TiO2) or water-soluble organic polymers such as poly[(9,9-dioctyl-2,7-fluorene)-alt-(9,9-bis(3'-(N,N-dimethylamino)propyl)-2,7-fluorene)] (PFN) and polyethylenimine ethoxylated (PEIE). In this work, we have developed a series of novel poly(ionic liquid) non-conjugated block copolymers for improving the performance of inverted organic photovoltaic cells (OPVs) by acting as work function modifiers of indium tin oxide (ITO) cathode...
January 17, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29333652/establishment-and-characterization-of-a-brain-cell-line-from-kelp-grouper-epinephelus-moara
#5
X F Liu, Y H Wu, S N Wei, N Wang, Y Z Li, N W Zhang, P F Li, Q W Qin, S L Chen
A new brain-cell line, EMB, was developed from kelp grouper Epinephelus moara, a cultured marine fish. The EMB cells were subcultured for more than 60 passages. The cells were cultured in Leibovitz's L-15 medium (L15) supplemented with antibiotics, foetal bovine serum (FBS), 2-mercaptoethanol (2-ME) and basic fibroblast growth factor (bFGF). The cells could grow at 18-30° C, with the maximum growth between 24 and 30° C. The optimum FBS concentration for the cells growth ranged between 15 and 20%. Chromosome analysis indicated that the modal chromosome number was 48 in the cells at passage 45...
January 14, 2018: Journal of Fish Biology
https://www.readbyqxmd.com/read/29330937/first-time-detection-of-porcine-reproductive-and-respiratory-syndrome-virus-prrsv-infection-in-uruguay
#6
N Ramos, S Mirazo, G Castro, K Cabrera, F Osorio, J Arbiza
Within the last two decades, several high-impact viruses have emerged in the global swine population, including porcine reproductive and respiratory syndrome virus (PRRSV). In Uruguay, the more recent serological survey for PRRSV and other notifiable diseases such as Aujeszky's disease virus (ADV) and classical swine fever virus (CSFV) dated from year 2000. The main purpose of this study was to update our information on the infection status of PRRSV, ADV and CSFV in Uruguayan pig herds, in order to keep informed about the epidemiological situation of these notifiable infections in the country...
January 12, 2018: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/29323993/why-you-should-be-aware-of-cpe-infection-and-transmission
#7
Martin Still
No abstract text is available yet for this article.
January 11, 2018: British Journal of Nursing: BJN
https://www.readbyqxmd.com/read/29316402/sorting-of-semiconducting-single-walled-carbon-nanotube-in-polar-solvents-with-an-amphiphilic-conjugated-polymer-provides-general-guidelines-for-enrichment
#8
Jianying Ouyang, Jianfu Ding, Jacques Lefebvre, Zhao Li, Chang Guo, Arnold J Kell, Patrick R L Malenfant
Conjugated polymer extraction (CPE) has been shown to be a highly effective method to isolate high purity semiconducting single-walled carbon nanotubes (sc-SWCNTs). In both literature reports and industrial manufacturing, this method has enabled enrichment of sc-SWCNTs with high purity (≥ 99.9%). High selectivity is typically obtained in non-polar aromatic solvents, yet polar solvents may provide process improvements in terms of yield, purity and efficiency. Using a novel amphiphilic fluorene-alt-pyridine conjugated copolymer with hydrophilic side chains we have investigated the enrichment of sc-SWCNTs in polar solvents...
January 9, 2018: ACS Nano
https://www.readbyqxmd.com/read/29315008/clostridium-perfringens-contamination-in-retail-meat-and-meat-based-products-in-bursa-turkey
#9
Artun Yibar, Ece Cetin, Zafer Ata, Evren Erkose, Mustafa Tayar
This study examined the incidence of Clostridium perfringens in raw, ready-to-cook (RTC), and ready-to-eat (RTE) meat and meat-based products (N = 306) collected from restaurants, supermarkets, and butcher shops in Bursa, Turkey. In addition, we investigated the presence of the C. perfringens enterotoxin (CPE), as well as cpe genes and their source (chromosomal or plasmid borne). In this study, tryptose sulfite cycloserine (TSC) agar for classic culture isolation and API and real-time polymerase chain reaction (RT-PCR) techniques were used to identify C...
January 9, 2018: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/29310297/selective-amperometric-flow-injection-analysis-of-carbofuran-using-a-molecularly-imprinted-polymer-and-gold-coated-magnetite-modified-carbon-nanotube-paste-electrode
#10
Maliwan Amatatongchai, Wongduan Sroysee, Purim Jarujamrus, Duangjai Nacapricha, Peter A Lieberzeit
Herein, we propose a new approach for selective determination of carbofuran (CBF) in vegetables, based on a simple flow-injection system using a molecularly-imprinted amperometric sensor. The sensor design is based on a carbon-paste electrode decorated with carbon nanotubes and gold-coated magnetite (CNTs-Fe3O4@Au/CPE) coated with a molecularly-imprinted polymer (MIP) for CBF sensing. The MIP was synthesized on the electrode surface by electropolymerization using a supramolecular complex, namely 4-ter-butylcalix [8] arene-CBF (4TB[8]A-CBF), as the template...
March 1, 2018: Talanta
https://www.readbyqxmd.com/read/29307649/diversity-of-toxin-genotypes-among-clostridium-perfringens-isolated-from-healthy-and-diarrheic-neonatal-cattle-and-buffalo-calves
#11
Athira Cheruplackal Karunakarnan, Arockiasamy Arun Prince Milton, Avinash Reddy, Arunraj Mekhemadhom Rajendrakumar, Abhishek, Med Ram Verma, Ashok Kumar, Viswas Konasagara Nagaleekar, Rajesh Kumar Agarwal
The diversity of toxin-genotypes of C. perfringens in neonatal calves was determined in this study. A total of 682 fresh faecal samples comprising 559 healthy and 123 diarrheic neonatal calves (cattle and buffalo) were collected from various farms in Northern India. The samples were processed for isolation of C. perfringens and toxin-genotyping by multiplex PCR. The overall prevalence of C. perfringens was 37.2%. The most predominant toxin-genotype was type A (59.7%) and the least prevalent was type C. There was no association between toxin genotypes and diarrhea of cattle and buffalo neonatal calves (P > ...
January 4, 2018: Anaerobe
https://www.readbyqxmd.com/read/29302083/forty-years-of-acpe-cpe-accreditation
#12
Dimitra V Travlos, Jennifer L Baumgartner, Mike Rouse, Jeffrey W Wadelin, Peter H Vlasses
The Accreditation Council for Pharmacy Education (ACPE) Continuing Pharmacy Education (CPE) Provider Accreditation Program has been in existence for 40 years. During this time, the program has expanded and has been offered to a various types of providers, not only academic-based providers. ACPE credit has been offered to an increasing number of pharmacists, pharmacy technicians, and other health professionals. This paper explains the evolution of the CPE Provider Accreditation Program, including the Definition of Continuing Education for the Profession of Pharmacy, its standards, types of activities (knowledge, application, and practice), CPE Monitor, Joint Accreditation for Interprofessional Continuing Education, and Continuing Professional Development (CPD)...
November 2017: American Journal of Pharmaceutical Education
https://www.readbyqxmd.com/read/29301512/epithelial-changes-with-corneal-punctate-epitheliopathy-in-type-2-diabetes-mellitus-and-their-correlation-with-time-to-healing
#13
Jing-Hao Qu, Li Li, Lei Tian, Xiao-Yu Zhang, Ravi Thomas, Xu-Guang Sun
BACKGROUND: To study basal epithelial cell (BEC), sub-basal nerve plexus (SBN) and Langerhans cell (LC) density in patients with type 2 diabetes mellitus (T2DM) with corneal punctate epitheliopathy (CPE) and to assess their association with time to healing of CPE. METHODS: Retrospective study of in vivo confocal microscopy (IVCM) in 160 eyes from 160 patients with T2DM diagnosed with CPE due to a single cause. Key exclusion criteria included multiple-causes for CPE or treatment with autologous serum...
January 4, 2018: BMC Ophthalmology
https://www.readbyqxmd.com/read/29298971/an-autopsy-case-of-mesenteric-panniculitis-with-massive-pleural-effusions
#14
Hiroshi Kobayashi, Kenji Notohara, Tadashi Otsuka, Yuka Kobayashi, Masuo Ujita, Yuuki Yoshioka, Naomasa Suzuki, Ryuji Aoyagi, Riuko Ohashi, Toshimitsu Suzuki
BACKGROUND Mesenteric panniculitis (MP) is an idiopathic chronic inflammatory condition of the mesentery. The main symptoms include abdominal pain, abdominal distention, weight loss, fever, nausea, and vomiting. The patients also present with chylous ascites in 14% of the cases and chylous pleural effusion (CPE) in very rare occasions. Despite the previous view of excellent prognosis of MP, two recent papers reported several fatal cases. However, there are still only a few autopsy case reports that describe the macroscopic and histological details of MP cases...
January 4, 2018: American Journal of Case Reports
https://www.readbyqxmd.com/read/29285901/characterization-of-porcine-sapelovirus-isolated-from-japanese-swine-with-plc-prf-5-cells
#15
H Bai, J Liu, L Fang, M Kataoka, N Takeda, T Wakita, T-C Li
Porcine sapelovirus (PSV) is a causative agent of neurological disorders, fertility disorders and dermal lesions of swine. In this study, we isolated two PSV strains, Jpsv477 and Jpsv1315, from swine faecal specimens using a PLC/PRF/5 cell culture system. The PSV infection of PLC/PRF/5 cells induced a cytopathic effect (CPE). Two types of virus particles with identical diameter (~35 nm) but different densities (1.300 and 1.285 g/cm3 ) were observed in the cell culture supernatants. Analysis of the entire genome sequence of Jpsv477 and Jpsv1315 revealed that both strains possess 7,558 nucleotides and the poly (A) tail and have a typical PSV genome organization consisting of a 5' terminal untranslated region (5'UTR), a large open reading frame (ORF), and a 3' terminal untranslated region (3'UTR)...
December 28, 2017: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/29285573/bacterial-lpx-motif-harboring-virulence-factors-constitute-a-species-spanning-family-of-cell-penetrating-effectors
#16
Stefanie Norkowski, Britta Körner, Lilo Greune, Anne-Sophie Stolle, Marie-Luise Lubos, Philip R Hardwidge, M Alexander Schmidt, Christian Rüter
Effector proteins are key virulence factors of pathogenic bacteria that target and subvert the functions of essential host defense mechanisms. Typically, these proteins are delivered into infected host cells via the type III secretion system (T3SS). Recently, however, several effector proteins have been found to enter host cells in a T3SS-independent manner thereby widening the potential range of these virulence factors. Prototypes of such bacteria-derived cell-penetrating effectors (CPEs) are the Yersinia enterocolitica-derived YopM as well as the Salmonella typhimurium effector SspH1...
December 28, 2017: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/29278755/molecular-characterization-and-phylogenetic-analysis-of-clostridium-perfringens-from-animals-and-their-environments-by-cpn60-ut-sequencing-analysis
#17
Shreya Das, Saugata Majumder, Chetna Mathur, Joseph Kingston J
Clostridium perfringens an ubiquitous environmental bacterium causes major food borne illnesses, digestive diseases and several soft tissue infections in humans and animals. In the present study, toxin typing of 91 C. perfringens isolates from animals with enteric diseases and their environments revealed the presence of type A and C strains. Enterotoxin gene (cpe), responsible for majority of the food poisoning incidences in humans and enteric infections in animals was present in 60.43% of the isolates of which 61...
December 23, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/29278329/insights-into-molecular-structures-and-optical-properties-of-stacked-au3-rn%C3%A2-cr-3-n-complexes
#18
Hassan Rabaâ, Mohammad A Omary, Stefan Taubert, Dage Sundholm
The molecular structure of stacked cyclic trinuclear gold(I) complexes [Au3(RN═CR')3]n, with n = 1-4, where R = H, methyl (Me), cyclopentyl (cPe), and phenyl (Ph) and R' = OH and methoxy (OMe) were studied computationally at the second-order Møller-Plesset (MP2) and density functional theory (DFT) levels of theory. At the DFT level, the aurophilic and dispersion interactions were accounted for by using the TPSS functional in combination with the semiempirical D3 correction. The structure optimizations yielded the lowest energy for a slided stacked structure of the [Au3(HN═COH)3]2 dimer, where monomers are slightly shifted relative to one another...
December 26, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/29277142/carriage-of-multidrug-resistant-bacteria-among-pediatric-patients-before-and-during-their-hospitalization-in-a-tertiary-pediatric-unit-in-tunisia
#19
Miniar Tfifha, Asma Ferjani, Manel Mallouli, Nesrine Mlika, Saoussen Abroug, Jalel Boukadida
The pandemic spread of multidrug-resistant (MDR) bacteria (i.e., methicillin-resistant Staphylococcus aureus (MRSA), extended-spectrum b-lactamase-producing Enterobacteriaceae (ESBLPE), vancomycin-resistant enterococci, carbapenemase-producing Enterobacteriaceae (CPE), multiresistant Pseudomonas aeruginosa and multiresistant Acinetobacter baumannii) pose a threat to healthcare Worldwide. We found limited data of MDR bacteria in pediatric patients hospitalized in Tunisian tertiary healthcare.The aim of the study is to evaluate the acquisition rate of MDR acquisition during hospitalization and to explore some of the associated risk factors for both carriage and acquisition at the pediatric department, Sahloul University Hospital...
December 2018: Libyan Journal of Medicine
https://www.readbyqxmd.com/read/29275286/structural-identification-of-lipopeptide-biosurfactants-produced-by-bacillus-subtilis-strains-grown-on-the-media-obtained-from-renewable-natural-resources
#20
Katarzyna Paraszkiewicz, Przemysław Bernat, Anna Kuśmierska, Joanna Chojniak, Grażyna Płaza
The aim of the study was to identify and characterize lipopeptide (LP) biosurfactants produced by two Bacillus subtilis strains (KP7 and I'-1a) grown on various media prepared from renewable natural resources: two different brewery wastewaters (BW#4 and BW#6), 2% beet molasses (M), apple peels extract (APE) supplemented with 0.25% of yeast extract (YE) or 0.25% peptone (P), and similarly supplemented carrot peels extract (CPE). In all used media both strains retained their individual LP production signature characterized by surfactin and iturin overproduction exhibited by KP7 and I'-1a strain, respectively...
December 21, 2017: Journal of Environmental Management
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