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https://www.readbyqxmd.com/read/16684535/undetectable-levels-of-n6-methyl-adenine-in-mouse-dna-cloning-and-analysis-of-pred28-a-gene-coding-for-a-putative-mammalian-dna-adenine-methyltransferase
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David Ratel, Jean-Luc Ravanat, Marie-Pierre Charles, Nadine Platet, Lionel Breuillaud, Joël Lunardi, François Berger, Didier Wion
Three methylated bases, 5-methylcytosine, N4-methylcytosine and N6-methyladenine (m6A), can be found in DNA. However, to date, only 5-methylcytosine has been detected in mammalian genomes. To reinvestigate the presence of m6A in mammalian DNA, we used a highly sensitive method capable of detecting one N6-methyldeoxyadenosine per million nucleosides. Our results suggest that the total mouse genome contains, if any, less than 10(3) m6A. Experiments were next performed on PRED28, a putative mammalian N6-DNA methyltransferase...
May 29, 2006: FEBS Letters
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