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https://www.readbyqxmd.com/read/28918326/a-surrogate-analyte-based-liquid-chromatography-tandem-mass-spectrometry-method-for-the-determination-of-endogenous-cyclic-nucleotides-in-rat-brain
#1
Jie Chen, Ali Tabatabaei, Doug Zook, Yan Wang, Anne Danks, Kathe Stauber
A robust high-performance liquid chromatography tandem mass spectrometry (LC-MS/MS) assay was developed and qualified for the measurement of cyclic nucleotides (cNTs) in rat brain tissue. Stable isotopically labeled 3',5'-cyclic adenosine-(13)C5 monophosphate ((13)C5-cAMP) and 3',5'-cyclic guanosine-(13)C,(15)N2 monophosphate ((13)C(15)N2-cGMP) were used as surrogate analytes to measure endogenous 3',5'-cyclic adenosine monophosphate (cAMP) and 3',5'-cyclic guanosine monophosphate (cGMP). Pre-weighed frozen rat brain samples were rapidly homogenized in 0...
September 14, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28918314/empirical-research-evaluating-the-effects-of-non-traditional-approaches-to-enhancing-sleep-in-typical-and-clinical-children-and%C3%A2-young-people
#2
REVIEW
Karyn G France, Laurie K McLay, Jolene E Hunter, Madeline L S France
This paper examines the effects of non-traditional (non-behavioural and non-prescription pharmaceutical) approaches to sleep in children and young people (0-18 y). A systematic search identified 79 studies that met inclusion criteria. Seventeen percent of the studies were rated as having a conclusive level of evidence, forty-two percent with preponderant evidence and forty-one percent with only suggestive evidence. There were promising indications, with certain populations only, for aromatherapy, ketogenic diets, an elimination diet (few foods diet), elimination of cow's milk, avoidance of caffeine, tryptophan with adenosine and uridine, omega-3 and omega-6, valerian, music, osteopathic manipulation and white noise...
July 29, 2017: Sleep Medicine Reviews
https://www.readbyqxmd.com/read/28918141/validation-of-commercial-luminometry-swabs-for-total-bacteria-and-coliform-counts-in-colostrum-feeding-equipment
#3
D L Renaud, D F Kelton, S J LeBlanc, D B Haley, A B Jalbert, T F Duffield
A sufficient quantity and quality of colostrum must be fed quickly to the newborn calf while minimizing bacterial contamination. Adenosine triphosphate bioluminescence swabs offer a potential rapid on-farm alternative to assess bacterial contamination of colostrum. The objective of this study was to validate the Hygiena (Camarillo, CA) AquaSnap Total (AS), SuperSnap (SS), PRO-Clean (PC), and MicroSnap Coliform (MS) swabs as well as visual hygiene assessment for detection of elevated bacterial counts in or on colostrum-feeding equipment...
September 13, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28917803/atp-mediated-rolling-circle-amplification-and-opening-dna-gate-for-drug-delivery-to-cell
#4
Yujie Wang, Xinxin Shang, Jia Liu, Yingshu Guo
Here, we have developed a facile fluorometric system for the detection of adenosine triphosphate (ATP) by a rolling circle amplification (RCA) based on proximity ligation mediated amplification, and simultaneously achieved the release of the anticancer drug doxorubicin (DOX) through the mesoporous silicon system. Once the ATP molecule is present, the linker DNA will be released from the graphene oxide (GO) surface and hybridized to the template DNA of the GO surface joining with ligation enzyme. RCA reaction is followed by the addition of the phi29 DNA polymerase...
January 1, 2018: Talanta
https://www.readbyqxmd.com/read/28917665/improving-mitochondrial-function-significantly-reduces-metabolic-visual-motor-and-cognitive-decline-in-aged-drosophila-melanogaster
#5
Tobias W Weinrich, Ariathney Coyne, Thomas E Salt, Christopher Hogg, Glen Jeffery
Mitochondria play a major role in aging. Over time, mutations accumulate in mitochondrial DNA leading to reduced adenosine triphosphate (ATP) production and increased production of damaging reactive oxygen species. If cells fail to cope, they die. Reduced ATP will result in declining cellular membrane potentials leading to reduced central nervous system function. However, aged mitochondrial function is improved by long wavelength light (670 nm) absorbed by cytochrome c oxidase in mitochondrial respiration...
August 30, 2017: Neurobiology of Aging
https://www.readbyqxmd.com/read/28917552/crispr-correction-of-the-prkag2-gene-mutation-in-the-patient-s-ipsc-derived-cardiomyocytes-eliminates-the-electrophysiological-and-structural-abnormalities
#6
Ronen Ben Jehuda, Binyamin Eisen, Yuval Shemer, Lucy N Mekies, Agnes Szantai, Irina Reiter, Huanhuan Cui, Kaomei Guan, Shiraz Haron-Khun, Dov Freimark, Silke R Sperling, Mihaela Gherghiceanu, Michael Arad, Ofer Binah
BACKGROUND: Mutations in the PRKAG2 gene encoding the γ-subunit of adenosine monophosphate-kinase (AMPK) cause hypertrophic cardiomyopathy (HCM) and familial-Wolff-Parkinson-White syndrome (WPW). Patients carrying the R302Q mutation in PRKAG2 present sinus bradycardia, escape rhythms, ventricular pre-excitation, supraventricular tachycardia and atrioventricular block. This mutation affects AMPK activity and increases glycogen storage in cardiomyocytes. The link between glycogen storage, WPW, HCM and arrhythmias remains unknown...
September 13, 2017: Heart Rhythm: the Official Journal of the Heart Rhythm Society
https://www.readbyqxmd.com/read/28916910/encompassing-atp-dna-insulin-and-protein-content-for-quantification-and-assessment-of-human-pancreatic-islets
#7
Meirigeng Qi, Shiela Bilbao, Elena Forouhar, Fouad Kandeel, Ismail H Al-Abdullah
Islet transplantation has made major progress to treat patients with type 1 diabetes. Islet mass and quality are critically important to ensure successful transplantation. Currently, islet status is evaluated using insulin secretion, oxygen consumption rate, or adenosine triphosphate (ATP) measurement. These parameters are evaluated independently and do not effectively predict islet status post-transplant. Therefore, assessing human pancreatic islets by encompassing ATP, DNA, insulin, and protein content from a single tissue sample would serve as a better predictor for islet status...
September 15, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28916770/changes-in-cd73-cd39-and-cd26-expression-on-t-lymphocytes-of-anca-associated-vasculitis-patients-suggest-impairment-in-adenosine-generation-and-turn-over
#8
Lovis Kling, Urs Benck, Annette Breedijk, Lisa Leikeim, Marianne Heitzmann, Stefan Porubsky, Bernhard K Krämer, Benito A Yard, Anna-Isabelle Kälsch
Extracellular adenosine, generated via the concerted action of CD39 and CD73, contributes to T-cell differentiation and function. Adenosine concentrations are furthermore influenced by adenosine deaminase binding protein CD26. Because aberrant T-cell phenotypes had been reported in anti-neutrophil cytoplasmic auto-antibody (ANCA)-associated vasculitis (AAV) patients, an impaired expression of these molecules on T-cells of AAV patients was hypothesized in the present study. While in AAV patients (n = 29) CD26 was increased on CD4(+) lymphocytes, CD39 and CD73 were generally reduced on patients' T-cells...
September 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28916378/clinical-pharmacology-of-anti-angiogenic-drugs-in-oncology
#9
REVIEW
P Gougis, J Wassermann, J P Spano, N Keynan, C Funck-Brentano, J E Salem
Abnormal vasculature proliferation is one of the so-called hallmarks of cancer. Angiogenesis inhibitor therapies are one of the major breakthroughs in cancer treatment in the last two decades. Two types of anti-angiogenics have been approved: monoclonal antibodies and derivatives, which are injected and target the extracellular part of a receptor, and protein kinase inhibitors, which are orally taken small molecules targeting the intra-cellular Adenosine Triphosphate -pocket of different kinases. They have become an important part of some tumors' treatment, both in monotherapy or in combination...
September 1, 2017: Critical Reviews in Oncology/hematology
https://www.readbyqxmd.com/read/28916044/antibiotic-affinity-strategy-for-bioluminescent-detection-of-viable-gram-positive-bacteria-using-daptomycin-as-recognition-agent
#10
Mengyao Wang, Yue Wu, Yong He, Xiaoxiao Su, Hui Ouyang, Zhifeng Fu
A bioluminescent method was proposed for rapid detection of viable Gram-positive bacteria based on a novel antibiotic-affinity strategy on a magnetic beads (MBs) platform. Daptomycin, a highly efficient lipopeptide antibiotic for Gram-positive bacteria, was used as a recognition agent to functionalize MBs. The daptomycin-functionalized MBs showed high capture and concentration efficiency for Gram-positive bacteria due to the strong binding between daptomycin and bacterial cell membrane in the presence of Ca(2+) ion...
September 22, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28915999/oxidative-stress-in-dairy-cows-seropositives-for-neospora-caninum
#11
Patrícia Glombowsky, Nathieli B Bottari, Vanderlei Klauck, Juscivete F Fávero, Natan M Soldá, Matheus D Baldissera, Gessica Perin, Vera M Morsch, Maria Rosa C Schetinger, Lenita M Stefani, Aleksandro S Da Silva
Bovine neosporosis is caused by the protozoan Neospora caninum and is one of the major causes of abortion in cows. Cattle are intermediate hosts of this parasite and may have asymptomatic or symptomatic infections. Therefore, the aim of this study was to evaluate oxidative stress marker reactive oxygen species (ROS), thiobarbituric reactive acid substances (TBARS) levels, glutathione S-transferase (GST), adenosine deaminase (ADA), and butyrylcholinesterase (BChE) activities in dairy cows seropositives for N...
October 2017: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/28915341/-31-p-magnetic-resonance-fingerprinting-for-rapid-quantification-of-creatine-kinase-reaction-rate-in-vivo
#12
Charlie Y Wang, Yuchi Liu, Shuying Huang, Mark A Griswold, Nicole Seiberlich, Xin Yu
The purpose of this work was to develop a (31) P spectroscopic magnetic resonance fingerprinting (MRF) method for fast quantification of the chemical exchange rate between phosphocreatine (PCr) and adenosine triphosphate (ATP) via creatine kinase (CK). A (31) P MRF sequence (CK-MRF) was developed to quantify the forward rate constant of ATP synthesis via CK ( kfCK), the T1 relaxation time of PCr ( T1PCr), and the PCr-to-ATP concentration ratio ( MRPCr). The CK-MRF sequence used a balanced steady-state free precession (bSSFP)-type excitation with ramped flip angles and a unique saturation scheme sensitive to the exchange between PCr and γATP...
September 15, 2017: NMR in Biomedicine
https://www.readbyqxmd.com/read/28914074/impact-of-test-conditions-on-adp-induced-platelet-function-results-with-the-multiplate-assay-is-further-standardization-required
#13
Döme A Dézsi, Béla Merkely, Judit Skopál, Eszter Barabás, Katalin Várnai, József Faluközy, Gábor Veress, Nasri Alotti, Dániel Aradi
BACKGROUND: Platelet function testing was suggested to help tailor P2Y12-inhibitor therapy; however, the lack of proper standardization is still a limitation. METHODS: In a prospective study, we enrolled clopidogrel-treated and P2Y12-inhibitor naive patients to investigate the influence of (1) time from blood collection, (2) stability of the stored Adenosine diphosphate (ADP) reagent, and (3) the use of enoxaparin on results of the Multiplate assay. Measurements were performed from samples kept for 0, 30, 60, 120, and 240 minutes at room temperature before processing...
January 1, 2017: Journal of Cardiovascular Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/28914001/comparative-study-of-the-effects-of-trabecular-meshwork-outflow-drugs-on-the-permeability-and-nitric-oxide-production-in-trabecular-meshwork-cells
#14
Jae Woo Kim
PURPOSE: To compare the effects of the barrier function in human trabecular meshwork (TM) cells monolayer and the production of nitric oxide (NO) between trabecular outflow drugs, Rho-associated kinase (ROCK) inhibitors, adenosine, and statin. METHODS: Primary cultured TM cells were exposed to 10 or 25 μM Y-27632, 0.1 or 1 μM N6-cyclohexyladenosine (CHA), or 15 or 30 μM simvastatin for 24 hours. NO production and expression of endothelial nitric oxide synthase mRNA were measured by Griess assay and reverse transcription polymerase chain reaction, respectively...
July 25, 2017: Korean Journal of Ophthalmology: KJO
https://www.readbyqxmd.com/read/28913856/multiplate-and-teg-platelet-mapping-in-a-population-of-severely-injured-trauma-patients
#15
M J George, J Burchfield, B MacFarlane, Y-W W Wang, J C Cardenas, N J White, B S Gill, C E Wade
OBJECTIVES: The objectives of this study were to compare thromboelastography platelet mapping (TEG PM) with impedance aggregometry (Multiplate, MP) in a single trauma population and relate their results clinically. BACKGROUND: Platelet function as measured by thromboelastography and impedance aggregometry demonstrates significant reductions that persist for days following traumatic injury. However, no study compares these devices and the correlation between them is not known...
September 15, 2017: Transfusion Medicine
https://www.readbyqxmd.com/read/28913424/reca-filament-maintains-structural-integrity-using-atp-driven-internal-dynamics
#16
Sung Hyun Kim, TakKyoon Ahn, Tao Ju Cui, Sweeny Chauhan, Jaeyoung Sung, Chirlmin Joo, Doseok Kim
At the core of homologous DNA repair, RecA catalyzes the strand exchange reaction. This process is initiated by a RecA loading protein, which nucleates clusters of RecA proteins on single-stranded DNA. Each cluster grows to cover the single-stranded DNA but may leave 1- to 2-nucleotide (nt) gaps between the clusters due to three different structural phases of the nucleoprotein filaments. It remains to be revealed how RecA proteins eliminate the gaps to make a seamless kilobase-long filament. We develop a single-molecule fluorescence assay to observe the novel internal dynamics of the RecA filament...
September 2017: Science Advances
https://www.readbyqxmd.com/read/28913351/adequate-platelet-function-inhibition-confirmed-by-two-inductive-agents-predicts-lower-recurrence-of-ischemic-stroke-transient-ischemic-attack
#17
Lulu Zhang, Xiaowei Hu, Juehua Zhu, Xiuying Cai, Yan Kong, Hui Wang, Shanshan Diao, Hongru Zhao, Jianhua Jiang, Dapeng Wang, Quanquan Zhang, Yiren Qin, Wei Yue, Qi Fang
BACKGROUND: The correlation between platelet function and recurrent ischemic stroke or TIA remains uncertain. OBJECTIVE: To investigate two inductive agents to detect platelet functions and assess associations with recurrent ischemic stroke/TIA. METHOD: The study included 738 ischemic stroke/TIA patients. On days 0, 3, and 9 after antiplatelet therapy, platelet function tests were determined by maximum aggregation rate (MAR) using a PL-11 platelet function analyzer and phase matching reagents...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28913151/harlequin-ichthyosis-a-rare-case
#18
Belide Shruthi, B R Nilgar, Anita Dalal, Nehaben Limbani
Harlequin ichthyosis is a very rare condition that affects the skin of newborns. It is associated with poor barrier function of the skin leading to dehydration and leaves newborns prone to infections. It is due to mutations in adenosine triphosphate binding cassette A12 gene transmitted as an autosomal recessive disorder. The prognosis is very poor in these cases. Here, we report one such rare case.
June 2017: Turk J Obstet Gynecol
https://www.readbyqxmd.com/read/28912867/protective-effect-of-controlled-release-of-cytokine-response-modifier-a-from-chitosan-microspheres-on-rat-chondrocytes-from-interleukin-1%C3%AE-induced-inflammation-and-apoptosis
#19
Pang-Hu Zhou, Lei Shi, Bo Qiu
The aim of the present study was to investigate the protective effect of cytokine response modifier A (CrmA) released from chitosan (CS) microspheres in a controlled manner on interleukin (IL)-1β-induced inflammation and apoptosis in chondrocytes. The CrmA release kinetics were characterized by an initial burst release, which was reduced to a linear release over 8 days. Furthermore, chondrocytes were isolated from 1-week-old Sprague Dawley rats. The cell culture was established by stimulation with 10 ng/ml IL-1β and subsequent incubation with CS-CrmA microspheres...
October 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28912724/abnormalities-in-the-polysomnographic-adenosine-and-metabolic-response-to-sleep-deprivation-in-an-animal-model-of-hyperammonemia
#20
Selena Marini, Olena Santangeli, Pirjo Saarelainen, Benita Middleton, Namrata Chowdhury, Debra J Skene, Rodolfo Costa, Tarja Porkka-Heiskanen, Sara Montagnese
Patients with liver cirrhosis can develop hyperammonemia and hepatic encephalopathy (HE), accompanied by pronounced daytime sleepiness. Previous studies with healthy volunteers show that experimental increase in blood ammonium levels increases sleepiness and slows the waking electroencephalogram. As ammonium increases adenosine levels in vitro, and adenosine is a known regulator of sleep/wake homeostasis, we hypothesized that the sleepiness-inducing effect of ammonium is mediated by adenosine. Eight adult male Wistar rats were fed with an ammonium-enriched diet for 4 weeks; eight rats on standard diet served as controls...
2017: Frontiers in Physiology
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