Ribeye

This study investigated the effects of a novel steak fabrication method, involving removal of both subcutaneous and intermuscular fats between the longissimus thoracis (LT) and spinalis dorsi (SD) muscles before cooking, on beef ribeye steak palatability and calorie content. Canada AA (n = 10) and AAA (n = 10) ribeyes were fabricated into steaks either with (fat-on) or without (fat-off) subcutaneous and intermuscular fats. Fat-on steaks had shorter cooking times and lower cooking losses than fat-off steaks (P < 0...

Moderate noise exposure induces cochlear synaptopathy, the loss of afferent ribbon synapses between cochlear hair cells and spiral ganglion neurons, which is associated with functional hearing decline. Prior studies have demonstrated noise-induced changes in the distribution and number of synaptic components, but the dynamic changes that occur after noise exposure have not been directly visualized. Here, we describe a live imaging model using RIBEYE-tagRFP to enable direct observation of pre-synaptic ribbons in mature hearing mouse cochleae after synaptopathic noise exposure...

The rumen microbiota is important for energy and nutrient acquisition in cattle, and therefore its composition may also affect carcass merit and meat quality attributes. In this study, we examined the associations between archaeal and bacterial taxa in the rumen microbiota of beef cattle and 12 different attributes, including hot carcass weight (HCW), dressing percentage, ribeye area (REA), intramuscular fat content, marbling score, fat thickness, yield grade, moisture content, purge loss, and shear force. There were significant correlations between the relative abundance of certain archaeal and bacterial genera and these attributes...

Beef × dairy crossbred cattle (n=615) were used to evaluate the effect of pre-harvest indicator traits and genotypes on accuracy of estimated breeding values (EBV) of seedstock candidates for selection. Genotypes for 100,000 single nucleotide polymorphisms (SNP) were provided by the American Simmental Association of purebred and crossbred seedstock animals (n=2,632). Five hundred ninety-five of the 615 beef x dairy cattle had carcass camera and ultrasound data. Phenotypes were not used for any of the seedstock animals even though some may have had performance and ultrasound data...

The objectives of this study were: 1) to understand stakeholder perceptions regarding the bison industry, 2) to benchmark live animal characteristics and production parameters of the bison industry, and 3) to identify live animal factors related to animal welfare and their effect on specific quality characteristics of bison meat. A survey was conducted both online and in-person at the National Bison Association Winter Conference (2022). Descriptive statistics were performed on a total of 110 surveys. Most stakeholders (94%, n = 104) agreed that the industry should continue to grow, and the majority (99%, 108) agreed that animal welfare impacts meat quality...

We generated a novel Cre mouse strain for cell-specific deletion of floxed genes in ribbon synapse-forming retinal neurons. Previous studies have shown that the RIBEYE promotor targets the expression of recombinant proteins such as fluorescently tagged RIBEYE to photoreceptors and retinal bipolar cells and generates fluorescent synaptic ribbons in situ in these neurons. Here, we used the same promotor to generate a novel transgenic mouse strain in which the RIBEYE promotor controls the expression of a Cre-ER(T2) recombinase (RIBEYE-Cre)...

UNLABELLED: Non-spiking sensory hair cells of the auditory and vestibular systems encode a dynamic range of graded signals with high fidelity by vesicle exocytosis at ribbon synapses. Ribeye, the most abundant protein in the synaptic ribbon, is composed of a unique A domain specific for ribbons and a B-domain nearly identical to the transcriptional corepressor CtBP2. CTBP2 and the B-domain of Ribeye contain a surface cleft that binds to proteins harboring a PXDLS/T peptide motif. Little is known about the importance of this binding site in synaptic function...

Our objective was to use measures of intake and productive performance to adjust prediction models for the carcass traits of non-castrated Nellore cattle finished in a feedlot. Individual data from 168 non-castrated male Nellore steers finished in feedlot between the years 2016-2021 were used. Descriptive statistical analyzes and Pearson correlation coefficients were performed. The outliers were tested by evaluating the studentized residuals in relation to the values predicted by the equations. Residues that were outside the range of -2...

Cobalt is an essential trace mineral required for ruminal vitamin B12 synthesis, but sources differ in ruminal microbial utilization, i.e., cobalt carbonate is poorly water soluble, whereas acetate and lactate forms are water soluble. Reports comparing organic cobalt lactate to other cobalt salts are lacking. The study objective was to determine if feeding cobalt lactate at two inclusion rates resulted in similar growth performance and tissue cobalt concentrations as the carbonate and acetate forms used in feeds...

This study explored the impacts of sire and dam breed on carcass quality and composition in a pasture-based system and the use of DXA to rapidly rank carcasses for leanness. Southdown (SD) and Suffolk (SF) ewes were mated to Texel (TX) or SD rams to produce seventy-nine lambs. Lambs were raised on pasture-based systems with limited grain supplementation. Lamb birth weight was greater ( p < 0.01) for TX, regardless of dam breed. Lambing rate was lower ( p < 0.01) for SD than SF ewes. Circulating myostatin concentrations were greater ( p < 0...

Beef genetics are used with increasing frequency on commercial dairies. Although use of beef genetics improves calf value, variability has been reported in beef × dairy calf phenotype for traits related to muscularity and carcass composition. The objective of this study was to characterize morphometric and compositional differences between beef, beef × dairy, and dairy fed cattle. Tested treatment groups included Angus-sired straightbred beef steers and heifers (A×B; n = 45), Angus × Holstein crossbreds (A×H; n = 15), Angus × Jersey crossbreds (A×J; n = 16), and straightbred Holsteins (H, n = 16)...

BACKGROUND: The concept of the functional nutritional value of health-beneficial omega-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFA) is becoming a phenomenon among red meat consumers globally. This study examined the expressions of three lipogenic genes (fatty acid binding protein 4, FABP4, fatty acid synthase, FASN; and stearoyl-CoA desaturase, SCD) in the ribeye (Longissimus thoracis et lumborum) muscle of Tattykeel Australian White (TAW) lambs fed fortified omega-3 diets and correlations with fatty acids...

The three otolithic endorgans of the inner ear are known to be involved in sound detection in different teleost fishes, yet their relative roles for auditory-vestibular functions within the same species remain unclear. In zebrafish (Danio rerio), saccule and utricle are thought to play key functions in encoding auditory and vestibular information, respectively, but the biological function of the lagena is not clear. We hypothesized that the zebrafish saccule serves as a primary auditory endorgan, making it more vulnerable to noise exposure, and that lagena might have an auditory function given its connectivity to the saccule and dominant vestibular function of the utricle...

Producer live performance data and carcasses from steers (n=116) resulting from the mating of four Limousin/Angus sires heterozygous for the F94L myostatin mutation to Jersey/Holstein dams were utilized to evaluate the effects of one copy of the F94L allele on live performance, carcass traits and USDA grades, and boxed beef and retail yields. Slaughter data were collected at time of harvest and carcass data was collected 48 hours postmortem. One side from each of 58 carcasses was fabricated into boxed beef and retail cuts by experienced lab personnel 5-8 days postmortem...

Pulmonary arterial pressure (PAP) can be used as an indicator of susceptibility to pulmonary hypertension and subsequent potential to develop right-sided heart failure (RHF). Previously reported heritability estimates of PAP have been moderate to high. Based on these estimates, selection for the indicator trait, PAP, could reduce the incidence of RHF due to hypoxia. Previous studies have also speculated that increased growth rates and body fat accumulation contribute to increased PAP and RHF. Research evaluating the genetic relationships between PAP and performance traits (e...

The objective of this study was to determine the influence that a ruminally-protected B-vitamin (RPBV) blend (containing vitamin B5, B6, B7, B9, and B12) had on growth performance, efficiency of dietary net energy utilization, carcass trait responses, and liver abscess severity and prevalence in beef steers fed a finishing diet. Steers ( n  = 246; initial shrunk body weight [BW] = 411 ± 25.8 kg) from two different sources, were used in a 126-d RCBD experiment. Within 48 h after arrival, steers were individually weighed and allotted to 1 of 24 pens ( n  = 8 to 12 steers; 8 pens per treatment) and randomly assigned to 1 of 3 treatments: (1) No RPBV; (2) RPBV1 at 1 g/steer d-1 ; 3) RPBV2 at 2 g/steer d-1 ...

This study aimed to evaluate the effects of different prenatal nutrition treatments on pregnant cows and their progeny. One hundred and twenty-six pregnant Nellore cows (455.3 ± 8.1 kg) were allocated in three different nutritional treatments during pregnancy: NP-control, PP-protein-energy supplementation in the last 3 months of pregnancy, and FP-the same supplementation throughout pregnancy. After parturition, all cows and calves received the same environmental and nutrition condition. The body condition score (BCS), body weight (BW), ribeye area (REA), backfat thickness (BFT), and rumpfat thickness (RFT) were collected on four occasions during pregnancy in the cows and from birth to finishing in calves...

The study's aim was to evaluate the effect of dietary Zn supplementation on steer performance, biomarkers of inflammation and metabolism, and liver abscess formation in response to a mild acidosis challenge. Forty-two steers (417 ± 3.99 kg; n = 6/pen) were housed in pens with bunks designed to measure individual dry matter intake ( DMI ) and fed one of two diets containing either 0 (CON; n = 18) or 90 mg Zn/kg from a Zn-amino acid complex ( Zn-AA ; n = 18; AvailaZn; Zinpro) for 109 d. Six additional steers were fed the CON diet and did not undergo the acidosis challenge ( NON ; n = 6)...

Wet distiller grains (WDG) are a corn by-product rich in protein and fiber that can be used in feedlot diets. This study evaluated F1 Angus-Nellore bulls fed on a control diet vs. WDG ( n = 25/treatment). After a period of 129 days on these feeds, the animals were slaughtered and Longissimus thoracis samples were collected for both a meat quality evaluation and gel-based proteomic analyses. A greater ribeye area (99.47 cm²) and higher carcass weight (333.6 kg) ( p < 0.05) were observed in the WDG-finished cattle compared to the control (80...

BACKGROUND: Intramuscular fat (IMF) is closely related to the tenderness, marbling, juiciness, and flavor of meat. We used a combined transcriptome and metabolome analysis to investigate the molecular mechanisms underlying phenotypic variation among Qinchuan cattle. RESULTS: The IMF content was relatively high in the meat of Qinchuan cattle bulls and differed among muscle locations, namely the high rib (15.86%), ribeye (14%), striploin (10.44%), and tenderloin (8...