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https://www.readbyqxmd.com/read/28214390/robust-peptidoglycan-growth-by-dynamic-and-variable-multi-protein-complexes
#1
REVIEW
Manuel Pazos, Katharina Peters, Waldemar Vollmer
In Gram-negative bacteria such as Escherichia coli the peptidoglycan sacculus resides in the periplasm, a compartment that experiences changes in pH value, osmolality, ion strength and other parameters depending on the cell's environment. Hence, the cell needs robust peptidoglycan growth mechanisms to grow and divide under different conditions. Here we propose a model according to which the cell achieves robust peptidoglycan growth by employing dynamic multi-protein complexes, which assemble with variable composition from freely diffusing sets of peptidoglycan synthases, hydrolases and their regulators, whereby the composition of the active complexes depends on the cell cycle state - cell elongation or division - and the periplasmic growth conditions...
February 15, 2017: Current Opinion in Microbiology
https://www.readbyqxmd.com/read/28214172/does-successful-rotator-cuff-repair-improve-muscle-atrophy-and-fatty-infiltration-of-the-rotator-cuff-a-retrospective-magnetic-resonance-imaging-study-performed-shortly-after-surgery-as-a-reference
#2
Noritaka Hamano, Atsushi Yamamoto, Hitoshi Shitara, Tsuyoshi Ichinose, Daisuke Shimoyama, Tsuyoshi Sasaki, Tsutomu Kobayashi, Yohei Kakuta, Toshihisa Osawa, Kenji Takagishi
BACKGROUND: Muscle atrophy and fatty infiltration in the rotator cuff muscles are often observed in patients with chronic rotator cuff tears. The recovery from these conditions has not been clarified. METHODS: Ninety-four patients were included in this study. The improvement in muscle atrophy and fatty infiltration in successfully repaired rotator cuff tears was evaluated by magnetic resonance imaging at 1 year and 2 years after surgery and was compared with muscle atrophy and fatty infiltration observed on magnetic resonance imaging at 2 weeks after surgery to discount any changes due to the medial retraction of the torn tendon...
February 15, 2017: Journal of Shoulder and Elbow Surgery
https://www.readbyqxmd.com/read/28213920/potato-aphid-macrosiphum-euphorbiae-performance-is-determined-by-aphid-genotype-and-not-mycorrhizal-fungi-or-water-availability
#3
Alison Jane Karley, Matthew Emslie-Smith, Alison Elizabeth Bennett
Intra- and inter-specific variation in plant and insect traits can alter the strength and direction of insect-plant interactions, with outcomes modified by soil biotic and abiotic conditions. We used the potato aphid (Macrosiphum euphorbiae Thomas) feeding on cultivated S. tuberosum and wild S. berthaulti to study the impact of water availability and plant mutualistic arbuscular mycorrhizal (AM) fungi on aphid performance and susceptibility to a parasitoid wasp (Aphidius ervi Haliday). Plants were grown under glass with live or sterile AM fungal spores and supplied with sufficient or reduced water supply...
February 18, 2017: Insect Science
https://www.readbyqxmd.com/read/28213825/pyrophosphate-hydrolysis-in-the-extremely-halophilic-archaeon-haloarcula-japonica-is-catalyzed-by-a-single-enzyme-with-a-broad-ionic-strength-range
#4
Satoshi Wakai, Akihiro Abe, Sotaro Fujii, Kaoru Nakasone, Yoshihiro Sambongi
The soluble protein fraction of the extremely halophilic archaeon Haloarcula japonica exhibits substantial inorganic pyrophosphate (PPi) hydrolysis activity in the presence of 2-4 M NaCl (Wakai et al, J Biol Chem 288:29247-29251, 2013), which provides high ionic strength (2-4). In this study, much higher PPi hydrolysis activity was unexpectedly detected, even with 0 M NaCl in the presence of 100-200 mM MgSO4, providing a much lower ionic strength of 0.4-0.8, in the same protein fraction. Na(+) and Mg(2+) ions were required for activity under high and low ionic strength conditions, respectively...
February 17, 2017: Extremophiles: Life Under Extreme Conditions
https://www.readbyqxmd.com/read/28213443/changes-in-appetitive-associative-strength-modulates-nucleus-accumbens-but-not-orbitofrontal-cortex-neuronal-ensemble-excitability
#5
Joseph Ziminski, Sabine Hessler, Gabriella Margetts-Smith, Meike C Sieburg, Hans S Crombag, Eisuke Koya
Cues that predict the availability of food rewards influence motivational states and elicit food-seeking behaviors. If a cue no longer predicts food availability, animals may adapt accordingly by inhibiting food seeking responses. Sparsely activated sets of neurons, coined neuronal ensembles, have been shown to encode the strength of reward-cue associations. While alterations in intrinsic excitability have been shown to underlie many learning and memory processes, little is known about these properties specifically on cue-activated neuronal ensembles...
February 17, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28213319/continuous-ohmic-heating-of-commercially-processed-apple-juice-using-five-sequential-electric-fields-results-in-rapid-inactivation-of-alicyclobacillus-acidoterrestris-spores
#6
N H Kim, J H Ryang, B S Lee, C T Kim, M S Rhee
Spores of Alicyclobacillus acidoterrestris, a spoilage bacterium, cause problems for the apple juice industry because they are resistant to thermal treatment. Here, we examined the sporicidal effect of an ohmic heating (OH) system with five sequential electric fields and compared it with that of conventional heating. Apple juice product (50kg) inoculated with A. acidoterrestris spores were subjected to OH (electric field strength=26.7V/cm; frequency=25kHz) at 85-100°C for 30-90s. The effect of conventional heating was also examined under these conditions...
January 27, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28213100/effect-of-processing-conditions-of-dicalcium-phosphate-cements-on-graft-resorption-and-bone-formation
#7
Zeeshan Sheikh, Yu Ling Zhang, Faleh Tamimi, Jake Barralet
Dicalcium phosphate cements (brushite and monetite) are resorbable biomaterials with osteoconductive potential for bone repair and regeneration that have yet to gain widespread commercial use. Brushite can be converted to monetite by heat treatments additionally resulting in various changes in the physico-chemical properties. However, since conversion is most commonly performed using autoclave sterilisation (wet heating), it is uncertain whether the properties observed for monetite as a result of heating brushite under dry conditions affect resorption and bone formation favourably...
February 14, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28212706/sclerostin-antibody-inhibits-skeletal-deterioration-in-mice-exposed-to-partial-weight-bearing
#8
J M Spatz, R Ellman, A M Cloutier, L Louis, M van Vliet, D Dwyer, M Stolina, H Z Ke, M L Bouxsein
Whereas much is known regarding the musculoskeletal responses to full unloading, little is known about the physiological effects and response to pharmacological agents in partial unloading (e.g. Moon and Mars) environments. To address this, we used a previously developed ground-based model of partial weight-bearing (PWB) that allows chronic exposure to reduced weight-bearing in mice to determine the effects of murine sclerostin antibody (SclAbII) on bone microstructure and strength across different levels of mechanical unloading...
February 2017: Life Sciences in Space Research
https://www.readbyqxmd.com/read/28212606/the-implementation-of-health-promotion-in-primary-and-community-care-a-qualitative-analysis-of-the-prescribe-vida-saludable-strategy
#9
Catalina Martinez, Gonzalo Bacigalupe, Josep M Cortada, Gonzalo Grandes, Alvaro Sanchez, Haizea Pombo, Paola Bully
BACKGROUND: The impact of lifestyle on health is undeniable and effective healthy lifestyle promotion interventions do exist. However, this is not a fundamental part of routine primary care clinical practice. We describe factors that determine changes in performance of primary health care centers involved in piloting the health promotion innovation 'Prescribe Vida Saludable' (PVS) phase II. METHODS: We engaged four primary health care centers of the Basque Healthcare Service in an action research project aimed at changing preventive health practices...
February 17, 2017: BMC Family Practice
https://www.readbyqxmd.com/read/28212265/variations-in-oxidative-stress-levels-in-3-days-follow-up-in-ultramarathon-mountain-race-athletes
#10
Ypatios Spanidis, Dimitrios Stagos, Marina Orfanou, Nikolaos Goutzourelas, David Bar-Or, Demetrios Spandidos, Demetrios Kouretas
Spanidis, Y, Stagos, D, Orfanou, M, Goutzourelas, N, Bar-or, D, Spandidos, D, and Kouretas, D. Variations in oxidative stress levels in 3 days follow-up in ultramarathon mountain race athletes. J Strength Cond Res 31(3): 582-594, 2017-The aim of the present study was the monitoring of the redox status of runners participating in a mountain ultramarathon race of 103 km. Blood samples from 12 runners were collected prerace and 24, 48, and 72 hours postrace. The samples were analyzed by using conventional oxidative stress markers, such as protein carbonyls (CARB), thiobarbituric acid reactive substances (TBARS), total antioxidant capacity (TAC) in plasma, as well as glutathione (GSH) levels and catalase (CAT) activity in erythrocytes...
March 2017: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/28211052/life-history-strategy-determines-constraints-on-immune-function
#11
Benjamin J Parker, Seth M Barribeau, Alice M Laughton, Lynn H Griffin, Nicole M Gerardo
1)Determining the factors governing investment in immunity is critical for understanding host-pathogen ecological and evolutionary dynamics. Studies often consider disease resistance in the context of life-history theory, with the expectation that investment in immunity will be optimized in anticipation of disease risk. Immunity, however, is constrained by context-dependent fitness costs. How the costs of immunity vary across life-history strategies has yet to be considered. 2)Pea aphids are typically unwinged but produce winged offspring in response to high population densities and deteriorating conditions...
February 17, 2017: Journal of Animal Ecology
https://www.readbyqxmd.com/read/28210042/the-effects-of-new-taping-methods-designed-to-increase-muscle-strength
#12
Tsutomu Fukui, Yuko Otake, Takashi Kondo
[Purpose] Although there are several studies on the use of elastic tape to influence muscle strength, results are contradictory and controversial. Our previous studies based on the sliding mechanism between superficial fascia and subcutaneous tissue may help the muscle strength. The purpose of this study was to confirm the effects of new taping methods on muscle strength. [Subjects and Methods] Sixteen healthy male participants took part in this study. Tape was applied on the right gluteus maximus and hip extension strength was determined by an isokinetic evaluation (30°/sec, concentric mode, four conditions)...
January 2017: Journal of Physical Therapy Science
https://www.readbyqxmd.com/read/28209146/heterologous-expression-of-aspergillus-aculeatus-endo-polygalacturonase-in-pichia-pastoris-by-high-cell-density-fermentation-and-its-application-in-textile-scouring
#13
Dede Abdulrachman, Paweena Thongkred, Kanokarn Kocharin, Monthon Nakpathom, Buppha Somboon, Nootsara Narumol, Verawat Champreda, Lily Eurwilaichitr, Antonius Suwanto, Thidarat Nimchua, Duriya Chantasingh
BACKGROUND: Removal of non-cellulosic impurities from cotton fabric, known as scouring, by conventional alkaline treatment causes environmental problems and reduces physical strength of fabrics. In this study, an endo-polygalacturonase (EndoPG) from Aspergillus aculeatus produced in Pichia pastoris was evaluated for its efficiency as a bioscouring agent while most current bioscouring process has been performed using crude pectinase preparation. RESULTS: The recombinant EndoPG exhibited a specific activity of 1892...
February 16, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28208997/comparison-of-self-etch-primers-with-conventional-acid-etching-system-on-orthodontic-brackets
#14
Amit Zope, Yogita Zope-Khalekar, Shrikant S Chitko, Veerendra V Kerudi, Harshal Ashok Patil, Prasad Vasudeo Bonde, Pratik Jaltare, Siddhesh G Dolas
INTRODUCTION: The self-etching primer system consists of etchant and primer dispersed in a single unit. The etching and priming are merged as a single step leading to fewer stages in bonding procedure and reduction in the number of steps that also reduces the chance of introduction of error, resulting in saving time for the clinician. It also results in smaller extent of enamel decalcification. AIM: To compare the Shear Bond Strength (SBS) of orthodontic bracket bonded with Self-Etch Primers (SEP) and conventional acid etching system and to study the surface appearance of teeth after debonding; etching with conventional acid etch and self-etch priming, using stereomicroscope...
December 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28208822/preparation-of-nanocellulose-reinforced-chitosan-films-cross-linked-by-adipic-acid
#15
Pouria Falamarzpour, Tayebeh Behzad, Akram Zamani
Adipic acid, an abundant and nontoxic compound, was used to dissolve and cross-link chitosan. After the preparation of chitosan films through casting technique, the in situ amidation reaction was performed at 80-100 °C as verified by Fourier transform infrared (FT-IR). The reaction was accompanied by the release of water which was employed to investigate the reaction kinetics. Accordingly, the reaction rate followed the first-order model and Arrhenius equation, and the activation energy was calculated to be 18 kJ/mol...
February 13, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28208604/reducing-fall-risk-with-combined-motor-and-cognitive-training-in-elderly-fallers
#16
Francesco Barban, Roberta Annicchiarico, Matteo Melideo, Alessia Federici, Maria Giovanna Lombardi, Simone Giuli, Claudia Ricci, Fulvia Adriano, Ivo Griffini, Manuel Silvestri, Massimo Chiusso, Sergio Neglia, Sergio Ariño-Blasco, Raquel Cuevas Perez, Yannis Dionyssiotis, Georgios Koumanakos, Milo Kovačeić, Nuria Montero-Fernández, Oscar Pino, Niels Boye, Ulises Cortés, Cristian Barrué, Atia Cortés, Peter Levene, Stelios Pantelopoulos, Roberto Rosso, José Antonio Serra-Rexach, Angelo Maria Sabatini, Carlo Caltagirone
BACKGROUND: Falling is a major clinical problem in elderly people, demanding effective solutions. At present, the only effective intervention is motor training of balance and strength. Executive function-based training (EFt) might be effective at preventing falls according to evidence showing a relationship between executive functions and gait abnormalities. The aim was to assess the effectiveness of a motor and a cognitive treatment developed within the EU co-funded project I-DONT-FALL...
February 10, 2017: Brain Sciences
https://www.readbyqxmd.com/read/28208500/sign-changes-as-a-universal-concept-in-first-passage-time-calculations
#17
Wilhelm Braun, Rüdiger Thul
First-passage-time problems are ubiquitous across many fields of study, including transport processes in semiconductors and biological synapses, evolutionary game theory and percolation. Despite their prominence, first-passage-time calculations have proven to be particularly challenging. Analytical results to date have often been obtained under strong conditions, leaving most of the exploration of first-passage-time problems to direct numerical computations. Here we present an analytical approach that allows the derivation of first-passage-time distributions for the wide class of nondifferentiable Gaussian processes...
January 2017: Physical Review. E
https://www.readbyqxmd.com/read/28208326/effects-of-flexoelectricity-and-weak-anchoring-on-a-freedericksz-transition-cell
#18
E Mema, L Kondic, L J Cummings
We consider a mathematical model that consists of a nematic liquid crystal layer sandwiched between two parallel bounding plates, across which an external field is applied. We investigate how the number and type of solutions for the director orientation within the layer change as the field strength, anchoring conditions, and material properties of the nematic liquid crystal layer vary. In particular, we focus on how the inclusion of flexoelectric effects alters the Freedericksz and saturation thresholds.
January 2017: Physical Review. E
https://www.readbyqxmd.com/read/28208148/local-and-global-cues-in-the-prosodic-realization-of-broad-and-narrow-focus-in-bulgarian
#19
Bistra Andreeva, William John Barry, Jacques Koreman
In this study, local and global prosodic cues for information structure are examined in the elicited production of six Bulgarian sentences. The sentences were produced in response to different questions, devised to prompt different focus realizations (broad focus and non-contrastive and contrastive narrow focus). Results show that speakers consistently differentiate broad and narrow focus by means of both local and global acoustic cues, by producing different pitch accent types on the nuclear syllable and reducing the 'phonetic strength' of the default pre-nuclear accent in the narrow focus condition...
February 23, 2017: Phonetica
https://www.readbyqxmd.com/read/28208129/stability-and-variability-in-slovak-prosodic-boundaries
#20
Štefan Benuš, Juraj Šimko
BACKGROUND/AIM: Encoding intended meanings in the type and strength of prosodic boundaries and strategies for communicating these meanings in ambient noise use similar prosodic cues. We analyze how increasing the level of ambient noise affects the realization of Slovak prosodic boundaries. METHODS: Five native speakers of Slovak read sentences, manipulating the boundary type (weak, rise, fall) and the location of pre-boundary pitch accent. Ambient noise of several levels was administered via headphones...
February 23, 2017: Phonetica
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