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Panel reactive antibody

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https://www.readbyqxmd.com/read/29152576/meningococcal-antigen-typing-system-mats-based-neisseria-meningitidis-serogroup-b-coverage-prediction-for-the-menb-4c-vaccine-in-the-united-states
#1
Gowrisankar Rajam, Maria Stella, Ellie Kim, Simon Paulos, Giuseppe Boccadifuoco, Laura Serino, George Carlone, Duccio Medini
Neisseria meningitidis is the most common cause of bacterial meningitis in children and young adults worldwide. A 4-component vaccine against N. meningitidis serogroup B (MenB) disease (MenB-4C [Bexsero]; GSK) combining factor H binding protein (fHBP), neisserial heparin binding protein (NHBA), neisserial adhesin A (NadA), and PorA-containing outer membrane vesicles was recently approved for use in the United States and other countries worldwide. Because the public health impact of MenB-4C in the United States is unclear, we used the meningococcal antigen typing system (MATS) to assess the strain coverage in a panel of strains representative of serogroup B (NmB) disease in the United States...
November 2017: MSphere
https://www.readbyqxmd.com/read/29143081/generation-in-yeast-and-antigenic-characterization-of-hepatitis-e-virus-capsid-protein-virus-like-particles
#2
Martynas Simanavicius, Paulius Lukas Tamosiunas, Rasa Petraityte-Burneikiene, Reimar Johne, Rainer G Ulrich, Aurelija Zvirbliene, Indre Kucinskaite-Kodze
Hepatitis E is a globally distributed human disease caused by hepatitis E virus (HEV). In Europe, it spreads through undercooked pork meat or other products and with blood components through transfusions. There are no approved or golden standard serologic systems for HEV diagnostics. Commercially available HEV tests often provide inconsistent results which may differ among the assays. In this study, we describe generation in yeast and characterization of HEV genotype 3 (HEV-3) and rat HEV capsid proteins self-assembled into virus-like particles (VLPs) and the development of HEV-specific monoclonal antibodies (MAbs)...
November 15, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29128424/the-use-of-synthetic-peptides-for-detection-of-anti-citrullinated-protein-antibodies-in-rheumatoid-arthritis
#3
Nicole Hartwig Trier, Bettina Eide Holm, Julie Heiden, Ole Slot, Henning Locht, Bente Jensen, Hanne Lindegaard, Anders Svendsen, Christoffer Tandrup Nielsen, Søren Jacobsen, Elke Theander, Gunnar Houen
Rheumatoid arthritis (RA) is an autoimmune disease of unknown etiology. A characteristic feature of RA is the presence of anti-citrullinated protein antibodies (ACPA). Since ACPAs are highly specific for RA and are often present before the onset of RA symptoms, they have become valuable diagnostic and prognostic. As a result, several assays for detection of ACPAs exist, which vary in sensitivity and specificity. In this study, we analyzed the reactivity of RA sera to selected peptides by solid-phase immunoassays in order to develop an ACPA assay with improved sensitivity and specificity...
November 8, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/29111117/identification-of-b-cell-recognized-linear-epitopes-in-a-snake-venom-serine-proteinase-from-the-central-american-bushmaster-lachesis-stenophrys
#4
M Madrigal, A Alape-Girón, E Barboza-Arguedas, W Aguilar-Ulloa, M Flores-Díaz
Snake venom serine proteinases are toxins that perturb hemostasis acting on proteins from the blood coagulation cascade, the fibrinolytic or the kallikrein-kinin system. Despite the relevance of these enzymes in envenomations by viper bites, the characterization of the antibody response to these toxins at the molecular level has not been previously addressed. In this work surface-located B cell recognized linear epitopes from a Lachesis stenophrys venom serine proteinase (UniProt accession number Q072L7) were predicted using an artificial neuronal network at the ABCpred server, the corresponding peptides were synthesized and their immunoreactivity was analyzed against a panel of experimental and therapeutic antivenoms...
October 27, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/29083957/differential-diagnosis-of-flavivirus-infections-in-horses-using-viral-envelope-protein-domain-iii-antigens-in-enzyme-linked-immunosorbent-assay
#5
Thisun B H Piyasena, Yin X Setoh, Jody Hobson-Peters, Natalie A Prow, Helle Bielefeldt-Ohmann, Alexander A Khromykh, David Perera, Mary J Cardosa, Peter D Kirkland, Roy A Hall
In Australia, infection of horses with the West Nile virus (WNV) or Murray Valley encephalitis virus (MVEV) occasionally results in severe neurological disease that cannot be clinically differentiated. Confirmatory serological tests to detect antibody specific for MVEV or WNV in horses are often hampered by cross-reactive antibodies induced to conserved epitopes on the envelope (E) protein. This study utilized bacterially expressed recombinant antigens derived from domain III of the E protein (rE-DIII) of MVEV and WNV, respectively, to determine whether these subunit antigens provided specific diagnostic markers of infection with these two viruses...
October 30, 2017: Vector Borne and Zoonotic Diseases
https://www.readbyqxmd.com/read/29075658/improving-the-antibody-based-evaluation-of-autoimmune-encephalitis
#6
Lindsey McCracken, Junxian Zhang, Maxwell Greene, Anne Crivaro, Joyce Gonzalez, Malek Kamoun, Eric Lancaster
OBJECTIVE: We tested whether antibody screening samples of patients with suspected autoimmune encephalitis with additional research assays would improve the detection of autoimmune encephalitis compared with standard clinical testing alone. METHODS: We examined 731 samples (333 CSF, 182 sera, and 108 pairs) from a cohort of 623 patients who were tested for CNS autoantibodies by the University of Pennsylvania clinical laboratory over a 24-month period with cell-based assays (CBAs) on commercially obtained slides of fixed cells for antibodies to NMDA receptor (NMDAR), α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR), γ-aminobutyric acid-B receptor (GABABR), leucine-rich glioma-inactivated 1 (LGI1), contactin-associated protein-like 2 (Caspr2), and glutamic acid decarboxylase (GAD65)...
November 2017: Neurology® Neuroimmunology & Neuroinflammation
https://www.readbyqxmd.com/read/29064957/an-evaluation-of-the-safety-and-efficacy-of-simultaneous-bilateral-nephrectomy-and-renal-transplantation-for-polycystic-kidney-disease-a-20-year-experience
#7
COMPARATIVE STUDY
Elliot I Grodstein, Nathan Baggett, Shawn Wayne, Glen Leverson, Anthony M D'Alessandro, Luis A Fernandez, David P Foley, Joshua D Mezrich, Jon S Odorico, Robert R Redfield, Hans W Sollinger, Dixon B Kaufman
BACKGROUND: Many strategies regarding timing of native nephrectomies exist for patients with symptomatic polycystic kidney disease (PCKD). METHODS: This is a single-center, retrospective study of 594 adults with PCKD who had renal transplants from 1994 to 2014. Three groups were analyzed: renal transplant-only recipients (tx alone), recipients of simultaneous bilateral nephrectomies and transplant (simultaneous), and recipients with pretransplant bilateral nephrectomies (pre)...
November 2017: Transplantation
https://www.readbyqxmd.com/read/29051051/brief-introduction-of-current-technologies-in-isolation-of-broadly-neutralizing-hiv-1-antibodies
#8
REVIEW
Zehua Sun, Lixin Yan, Jiansong Tang, Qian Qian, Jerica Lenberg, Dandan Zhu, Wan Liu, Kao Wu, Yilin Wang, Shiqiang Lu
HIV/AIDS has become a worldwide pandemic. Before an effective HIV-1 vaccine eliciting broadly neutralizing monoclonal antibodies (bnmAbs) is fully developed, passive immunization for prevention and treatment of HIV-1 infection may alleviate the burden caused by the pandemic. Among HIV-1 infected individuals, about 20% of them generated cross-reactive neutralizing antibodies two to four years after infection, the details of which could provide knowledge for effective vaccine design. Recent progress in techniques for isolation of human broadly neutralizing antibodies has facilitated the study of passive immunization...
October 16, 2017: Virus Research
https://www.readbyqxmd.com/read/29043827/stability-guidelines-for-dithiothreitol-treated-red-blood-cell-reagents-used-for-antibody-detection-methods-in-patients-treated-with-daratumumab
#9
Wendy L Disbro
Daratumumab (DARA), a drug used to treat patients with multiple myeloma, causes interference in pre-transfusion testing. Samples from patients receiving DARA exhibit panreactivity in antibody detection and identification tests with red blood cells (RBCs). Many hospitals are sending these samples to reference laboratories. Dithiothreitol (DTT), a sulfhydryl chemical treatment of RBCs, negates this reactivity. This study investigated the stability of the antigens on DTT-treated RBCs to determine if large quantities of RBCs could be treated at one time, stored, and used for testing at a later time...
September 2017: Immunohematology
https://www.readbyqxmd.com/read/29037453/establishment-of-calculated-panel-reactive-antibody-and-its-potential-benefits-in-improving-the-kidney-allocation-strategy-in-taiwan
#10
Ssu-Wen Shen, Chun-Kai Chang, Yi-Shun Gao, Pin-Jou Hsu, Shuo-Chueh Cheng, Fei-Yun Liu, Shyh-Chyi Lo
BACKGROUND/PURPOSE: Renal transplant candidates who are highly sensitized to human leukocyte antigens (HLAs) tend to wait longer to find a matched donor and have poor outcomes. Most organ-sharing programs prioritize highly sensitized patients in the allocation scoring system. The HLA sensitization status is traditionally evaluated by the panel-reactive antibody (PRA) assay. However, this assay is method dependent and does not consider the ethnic differences in HLA frequencies. A calculated PRA (cPRA), based on a population's HLA frequency and patients' unacceptable antigens (UAs), correctly estimates the percentage of donors suitable for candidates...
October 13, 2017: Journal of the Formosan Medical Association, Taiwan Yi Zhi
https://www.readbyqxmd.com/read/29018252/neutralization-of-zika-virus-by-germline-like-human-monoclonal-antibodies-targeting-cryptic-epitopes-on-envelope-domain-iii
#11
Yanling Wu, Shun Li, Lanying Du, Chunyu Wang, Peng Zou, Binbin Hong, Mengjiao Yuan, Xiaonan Ren, Wanbo Tai, Yu Kong, Chen Zhou, Lu Lu, Xiaohui Zhou, Shibo Jiang, Tianlei Ying
The Zika virus (ZIKV), a flavivirus transmitted by Aedes mosquitoes, has emerged as a global public health concern. Pre-existing cross-reactive antibodies against other flaviviruses could modulate immune responses to ZIKV infection by antibody-dependent enhancement, highlighting the importance of understanding the immunogenicity of the ZIKV envelope protein. In this study, we identified a panel of human monoclonal antibodies (mAbs) that target domain III (DIII) of the ZIKV envelope protein from a very large phage-display naive antibody library...
October 11, 2017: Emerging Microbes & Infections
https://www.readbyqxmd.com/read/28990274/optimizing-multiplexed-detections-of-diabetes-antibodies-via-quantitative-microfluidic-droplet-array
#12
Kai Duan, Gargi Ghosh, Joe Fujiou Lo
Sensitive, single volume detections of multiple diabetes antibodies can provide immunoprofiling and early screening of at-risk patients. To advance the state-of-the-art suspension assays for diabetes antibodies, porous hydrogel droplets are leveraged in microfluidic serpentine arrays to enhance reagent transport. This spatially multiplexed assay is applied to the detection of antibodies against insulin, glutamic acid decarboxylase, and insulinoma-associated protein 2. Optimization of assay protocol results in a shortened assay time of 2 h, with better than 20 pg mL Supporting Information detection limits across all three antibodies...
October 9, 2017: Small
https://www.readbyqxmd.com/read/28988608/routine-c4d-immunohistochemistry-in-cardiac-allografts-long-term-outcomes
#13
Aliya N Husain, Kamran M Mirza, Savitri E Fedson
BACKGROUND: In the past decade, C4d has emerged as a potential marker for antibody-mediated rejection (AMR); however, evidence on its use as a prognostic tool has been controversial. Although the International Society for Heart and Lung Transplantation guideline recommends early routine surveillance of C4d in heart transplantation, there is no consensus on its value in the pathologic assessment of AMR. Herein we present a correlation analysis of C4d immunoreactivity in endomyocardial biopsies with clinical cardiac dysfunction, cellular rejection, human leukocyte antigen (HLA) status, cardiac allograft vasculopathy (CAV) and death...
September 14, 2017: Journal of Heart and Lung Transplantation
https://www.readbyqxmd.com/read/28987549/potential-impact-of-the-4cmenb-vaccine-on-oropharyngeal-carriage-of-neisseria-meningitidis
#14
Raquel Abad, Verónica Medina, Maria Del Carmen Fariñas, Luis Martínez-Martínez, Stefania Bambini, A Dari, Duccio Medini, Mariagrazia Pizza, Julio A Vázquez
The analysis of the potential impact of the meningococcal vaccines in asymptomatic carriers has become one of the key aspects in the evaluation of new vaccines and of their impact on disease control. An important step in this direction is provided by the analysis of the sequence variability and surface-exposure of the 4CMenB (Bexsero®) vaccine antigens, as well as the cross-reactivity of vaccine induced antibodies, in isolates from healthy carriers. The Spanish Reference Laboratory, in collaboration with the University Hospital Marqués de Valdecilla in Santander (Spain), carried out a meningococcal carrier survey between May 2010 and April 2012 (population aged 4 to 19 years)...
October 5, 2017: Journal of Infection
https://www.readbyqxmd.com/read/28978710/cobra-ha-elicits-hemagglutination-inhibition-antibodies-against-a-panel-of-h3n2-influenza-virus-co-circulating-variants
#15
Terianne M Wong, James D Allen, Anne-Gaelle Bebin-Blackwell, Donald M Carter, Timothy Alefantis, Joshua DiNapoli, Harold Kleanthous, Ted M Ross
Each influenza season, a set of wild-type viruses, representing one H1N1, one H3N2, 1-2 influenza B isolates, are selected for inclusion in the annual seasonal influenza vaccine. In order to develop broadly reactive subtype specific influenza vaccines, a methodology called computationally optimized broadly reactive antigens (COBRA) was used to design novel hemagglutinin (HA) vaccine immunogens. COBRA technology was effectively used to design HA immunogens that elicited antibodies that neutralized H5N1 and H1N1 isolates...
October 4, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28978709/elicitation-of-protective-antibodies-against-a-broad-panel-of-h1n1-viruses-in-ferrets-pre-immune-to-historical-h1n1-influenza-viruses
#16
Donald M Carter, Christopher A Darby, Scott K Johnson, Michael A Carlock, Greg A Kirchenbaum, Joshua DiNapoli, Harold Kleanthous, Ted M Ross
Most pre-clinical animal studies test influenza vaccines in immunologically naïve animal models, even though the results of vaccination may not accurately reflect the effectiveness of vaccine candidates in humans that have pre-existing immunity to influenza. In this study, novel, broadly-reactive influenza vaccine candidates were assessed in pre-immune ferrets. These animals were infected with different H1N1 isolates before being vaccinated or infected with another influenza virus. Previously, our group has described the design and characterization of computationally optimized broadly reactive HA antigens (COBRA) for H1N1 isolates...
October 4, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28978509/development-of-an-extended-specificity-multiplex-immunoassay-for-detection-of-streptococcus-pneumoniae-serotype-specific-antigen-in-urine-using-human-monoclonal-antibodies
#17
Seyi D Eletu, Carmen L Sheppard, Elizabeth Thomas, Kenneth Smith, Priya Daniel, David J Litt, Wei Shen Lim, Norman K Fry
Current pneumococcal vaccines cover the most common 10 to 23 serotypes of the 92 presently described. However, with the increased usage of pneumococcal serotype-based vaccines the risk of serotype-replacement and an increase in disease caused by non-vaccine serotypes remains. Serotype surveillance of pneumococcal infection relies heavily on culture techniques, which are known to be insensitive, particularly in non-invasive disease.Pneumococcal serotype-specific urine assays offer an alternative method of serotyping both invasive and non-invasive disease...
October 4, 2017: Clinical and Vaccine Immunology: CVI
https://www.readbyqxmd.com/read/28970687/detection-of-t-and-b-cells-specific-complement-fixing-alloantibodies-using-flow-cytometry-a-diagnostic-approach-for-a-resource-limited-laboratory
#18
Dharmendra Jain, Pranav Dorwal, Amit Pande, Neetu Tyagi, Simmi Mehra, Vimarsh Raina
BACKGROUND AND OBJECTIVES: Various methods have been reported for the detection of antibodies in recipient sera, which can be human leukocyte antigens (HLAs) or non-HLA specific, complement- or noncomplement fixing, as well as donor T (HLA-Class-I) and/or B cell (HLA-Class-I and II) specific. These alloantibodies play a pivotal role in antibody-mediated renal transplantation rejection. Deposition of C4d in peritubular capillaries of a kidney biopsy is a marker of antibody-mediated rejection...
July 2017: Asian Journal of Transfusion Science
https://www.readbyqxmd.com/read/28970678/inra-a-new-high-frequency-antigen-in-the-indian-in023-blood-group-system
#19
Sanmukh R Joshi, Ankita Sheladiya, Kinjal V Mendapara-Dobariya
BACKGROUND: The INDIAN blood group system comprises 4 antigens sensitive to enzymes and 2-aminoethyl isothiouronium bromide (AET). AIM: The patient's antibody was investigated for its specificity to the high-frequency antigens (HFA) of this system. MATERIAL AND METHODS: Low ionic strength solution (LISS)-tube/LISS-indirect antiglobulin test (IAT) methods were used. The patient's red blood cells (RBCs) were tested with antisera to HFA. Her antibody was tested with RBCs lacking the HFA...
July 2017: Asian Journal of Transfusion Science
https://www.readbyqxmd.com/read/28964890/safety-tolerability-and-pharmacodynamics-of-an-anti-interleukin-1%C3%AE-%C3%AE-dual-variable-domain-immunoglobulin-in-patients-with-osteoarthritis-of-the-knee-a-randomized-phase-1-study
#20
S X Wang, S B Abramson, M Attur, M A Karsdal, R A Preston, C J Lozada, M P Kosloski, F Hong, P Jiang, M J Saltarelli, B A Hendrickson, J K Medema
OBJECTIVE: To investigate the safety, tolerability, pharmacokinetics, and pharmacodynamics of ABT-981, a human dual variable domain immunoglobulin simultaneously targeting interleukin (IL)-1α and IL-1β, in patients with knee osteoarthritis (OA). METHOD: This was a randomized, double-blind, placebo-controlled, single-center study of multiple subcutaneous (SC) injections of ABT-981 in patients with mild-to-moderate OA of the knee (NCT01668511). Three cohorts received ABT-981 (0...
September 28, 2017: Osteoarthritis and Cartilage
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