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Panel reactive antibody

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https://www.readbyqxmd.com/read/28444979/false-negative-single-antigen-bead-assay-is-it-always-an-effect-of-prozone
#1
Dharmendra Jain, Jui Choudhuri, Rajni Chauhan, Pranav Dorwal, Deepak Sharma, Aseem K Tiwari, Vimarsh Raina
BACKGROUND: Bead based flow cytometry and Luminex play a major role in identification of alloantibodies in renal transplant work-up. Strong sensitization events may lead to prozone phenomenon that can affect single antigen bead (SAB) assay and result in false negativity. However, this can also be due to high titer of other blocking antibodies. While methods like, heat inactivation, C1 inhibitor, Ethylene diamine tetra-acetic-acid and Dithio threitol treatment can remove interfering antibodies of complement and IgM, these methods are not optimal if false negativity is due to prozone effect, which is high titer of antibodies alone...
April 26, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28434810/comparative-performance-of-the-geenius%C3%A2-hiv-1-hiv-2-supplemental-test-in-florida-s-public-health-testing-population
#2
Sally Fordan, Berry Bennett, Meghan Lee, Susanne Crowe
BACKGROUND: The Centers for Disease Control and Prevention (CDC) published updated guidelines in 2014 for the laboratory diagnosis of HIV in the United States, which recommend use of a supplemental immunoassay (IA) that differentiates HIV-1 from HIV-2 after a repeatedly reactive HIV-1/2 antigen/antibody "Combo" screening test. In October 2014, Bio-Rad Laboratories introduced the FDA-cleared Geenius HIV-1/HIV-2 Supplemental assay and in July 2016, it replaced the Multispot HIV-1/HIV-2 differentiation rapid test as the second test in the HIV diagnostic algorithm...
April 6, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28422757/cross-reactive-dengue-human-monoclonal-antibody-prevents-severe-pathologies-and-death-from-zika-virus-infections
#3
Yiu-Wing Kam, Cheryl Yi-Pin Lee, Teck-Hui Teo, Shanshan W Howland, Siti Naqiah Amrun, Fok-Moon Lum, Peter See, Nicholas Qing-Rong Kng, Roland G Huber, Mei-Hui Xu, Heng-Liang Tan, Andre Choo, Sebastian Maurer-Stroh, Florent Ginhoux, Katja Fink, Cheng-I Wang, Lisa F P Ng, Laurent Rénia
Zika virus (ZIKV) infections have been linked with neurological complications and congenital Zika syndrome. Given the high level of homology between ZIKV and the related flavivirus dengue virus (DENV), we investigated the level of cross-reactivity with ZIKV using a panel of DENV human mAbs. A majority of the mAbs showed binding to ZIKV virions, with several exhibiting neutralizing capacities against ZIKV in vitro. Three of the best ZIKV-neutralizing mAbs were found to recognize diverse epitopes on the envelope (E) glycoprotein: the highly conserved fusion-loop peptide, a conformation-specific epitope on the E monomer, and a quaternary epitope on the virion surface...
April 20, 2017: JCI Insight
https://www.readbyqxmd.com/read/28394300/computational-identification-of-antibody-epitopes-on-the-dengue-virus-ns1-protein
#4
Martina L Jones, Fiona S Legge, Kebaneilwe Lebani, Stephen M Mahler, Paul R Young, Daniel Watterson, Herbert R Treutlein, Jun Zeng
We have previously described a method to predict antigenic epitopes on proteins recognized by specific antibodies. Here we have applied this method to identify epitopes on the NS1 proteins of the four Dengue virus serotypes (DENV1-4) that are bound by a small panel of monoclonal antibodies 1H7.4, 1G5.3 and Gus2. Several epitope regions were predicted for these antibodies and these were found to reflect the experimentally observed reactivities. The known binding epitopes on DENV2 for the antibodies 1H7.4 and 1G5...
April 10, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28389099/a-multi-national-european-cross-sectional-study-of-feline-calicivirus-epidemiology-diversity-and-vaccine-cross-reactivity
#5
Maria M Afonso, Gina L Pinchbeck, Shirley L Smith, Janet M Daly, Rosalind M Gaskell, Susan Dawson, Alan D Radford
BACKGROUND: Feline calicivirus (FCV) is an important pathogen of cats for which vaccination is regularly practised. Long-term use of established vaccine antigens raises the theoretical possibility that field viruses could become resistant. This study aimed to assess the current ability of the FCV-F9 vaccine strain to neutralise a randomly collected contemporary panel of FCV field strains collected prospectively in six European countries. METHODS: Veterinary practices (64) were randomly selected from six countries (UK, Sweden, Netherlands, Germany, France and Italy)...
May 9, 2017: Vaccine
https://www.readbyqxmd.com/read/28387330/profiling-the-neutralizing-antibody-response-in-chronically-hiv-1-crf07_bc-infected-intravenous-drug-users-na%C3%A3-ve-to-antiretroviral-therapy
#6
Xintao Hu, Yuanyuan Hu, Chunhong Zhao, Hongmei Gao, Kelli M Greene, Li Ren, Liying Ma, Yuhua Ruan, Marcella Sarzotti-Kelsoe, David C Montefiori, Kunxue Hong, Yiming Shao
Characterizing neutralizing antibody (NAb) responses in individuals infected with diverse HIV-1 strains is necessary to reveal the novel targets for regional preventive and therapeutic strategies development. We evaluated the prevalence, breadth, and potency of NAb responses in 98 CRF07_BC-infected individuals using a large, multi-subtype panel of 30 tier 2-3 Env-pseudotyped viruses. Furthermore, we compared the neutralization pattern of CRF07_BC-infected people with that of subtype B'-infected individuals in China...
April 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28380622/induction-of-broadly-cross-reactive-stalk-specific-antibody-responses-to-influenza-group-1-and-group-2-hemagglutinins-by-natural-h7n9-virus-infection-in-humans
#7
Lu Liu, Raffael Nachbagauer, Lingyan Zhu, Yang Huang, Xinci Xie, Shan Jin, Anli Zhang, Yanmin Wan, Ariana Hirsh, Di Tian, Xiaolin Shi, Zhaoguang Dong, Songhua Yuan, Yunwen Hu, Florian Krammer, Xiaoyan Zhang, Jianqing Xu
Background.: The outbreak of novel avian H7N9 influenza virus infections in China in 2013 has demonstrated the continuing threat posed by zoonotic pathogens. Deciphering the immune response during natural infection will guide future vaccine development. Methods.: We assessed the induction of heterosubtypic cross-reactive antibodies induced by H7N9 infection against a large panel of recombinant hemagglutinins and neuraminidases by quantitative enzyme-linked immunosorbent assay, and novel chimeric hemagglutinin constructs were used to dissect the anti-stalk or -head humoral immune response...
February 15, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28372891/performance-evaluation-of-the-fda-approved-determine%C3%A2-hiv-1-2-ag-ab-combo-assay-using-plasma-and-whole-blood-specimens
#8
Silvina Masciotra, Wei Luo, Emily Westheimer, Stephanie E Cohen, Cynthia L Gay, Laura Hall, Yi Pan, Philip J Peters, S Michele Owen
BACKGROUND: The Determine™ HIV-1/2 Ag/Ab Combo (DC) rapid test can identify HIV-1 infection earlier than rapid antibody-only tests in plasma specimens. OBJECTIVES: We compared the performance of DC with a laboratory-based antigen/antibody (Ag/Ab) combo assay in plasma and evaluated antigen reactivity in whole blood specimens. STUDY DESIGN: We tested by DC 508 plasma specimens collected in a prospective study and 107 sequential plasma and simulated whole blood specimens from 20 seroconversion panels...
March 24, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28372890/performance-evaluation-of-the-point-of-care-insti%C3%A2-hiv-1-2-antibody-test-in-early-and-established-hiv-infections
#9
Sarah Adams, Wei Luo, Laura Wesolowski, Stephanie E Cohen, Philip J Peters, S Michele Owen, Silvina Masciotra
BACKGROUND: The flow-through INSTI™ HIV-1/HIV-2 Rapid Antibody (INSTI) test is a 60s FDA-approved test for HIV-1 and HIV-2 antibody testing using whole blood and plasma. OBJECTIVE: We evaluated the performance of INSTI using plasma and simulated whole blood specimens. STUDY DESIGN: INSTI's performance in plasma specimens from commercial seroconversion panels was assessed by estimating the relative sensitivity using a 50% cumulative frequency analysis and by comparing its performance with other FDA-approved rapid tests (RTs)...
March 23, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28370432/antigenic-diversity-of-h5-highly-pathogenic-avian-influenza-viruses-of-clade-2-3-4-4-isolated-in-asia
#10
Ayako Ohkawara, Masatoshi Okamatsu, Makoto Ozawa, Duc-Huy Chu, Lam Thanh Nguyen, Takahiro Hiono, Keita Matsuno, Hiroshi Kida, Yoshihiro Sakoda
H5 highly pathogenic avian influenza viruses (HPAIVs) have spread in both poultry and wild birds since the late 2003. The continued circulation of HPAIVs in poultry in several regions of the world has led to antigenic drift. In this study, we analyzed the antigenic properties of H5 HPAIVs isolated in Asia using four neutralizing monoclonal antibodies (MAbs) recognizing the hemagglutinin, which were established using A/chicken/Kumamoto/1-7/2014 (H5N8), belonging to clade 2.3.4.4 and also using polyclonal antibodies...
March 30, 2017: Microbiology and Immunology
https://www.readbyqxmd.com/read/28348361/the-impact-of-donor-specific-anti-human-leukocyte-antigen-hla-antibody-rebound-on-the-risk-of-antibody-mediated-rejection-in-sensitized-kidney-transplant-recipients
#11
Kyo Won Lee, Jae Berm Park, Chan Woo Cho, Nuri Lee, Heejin Yoo, Kyunga Kim, Hyojun Park, Eun Suk Kang, Wooseong Huh, Sungjoo Kim
BACKGROUND Donor-specific anti-HLA antibody (DSA) detected on Luminex-based single antigen assay (LSA) has become the subject of desensitization based upon the results of previous studies. We retrospectively investigated the impact of preoperative DSA on the incidence of antibody mediated rejection (AMR) in patients desensitized using a protocol based on rituximab and rabbit antithymocyte globulin (rATG). MATERIAL AND METHODS Nine patients (Group 1, 9/327, 2.8%) were complement dependent cytotoxicity crossmatch (CDC-XM) positive and underwent desensitization with rituximab (375 mg/m²), intravenous immunoglobulin (IVIG; 400 mg/kg), plasmapheresis, and rATG...
March 28, 2017: Annals of Transplantation: Quarterly of the Polish Transplantation Society
https://www.readbyqxmd.com/read/28347312/phenotyping-and-comparing-the-immune-cell-populations-of-free-ranging-atlantic-bottlenose-dolphins-tursiops-truncatus-and-dolphins-under-human-care
#12
Mahyar Nouri-Shirazi, Brittany F Bible, Menghua Zeng, Saba Tamjidi, Gregory D Bossart
BACKGROUND: Studies suggest that free-ranging bottlenose dolphins exhibit a suppressed immune system because of exposure to contaminants or microorganisms. However, due to a lack of commercially available antibodies specific to marine mammal immune cell surface markers, the research has been indecisive. The purpose of this study was to identify cross-reactive terrestrial-specific antibodies in order to assess the changes in the immune cell populations of dolphins under human care and free-ranging dolphins...
March 27, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28341999/immunological-memory-to-hyperphosphorylated-tau-in-asymptomatic-individuals
#13
Gabriel Pascual, Jehangir S Wadia, Xueyong Zhu, Elissa Keogh, Başak Kükrer, Jeroen van Ameijde, Hanna Inganäs, Berdien Siregar, Gerrard Perdok, Otto Diefenbach, Tariq Nahar, Imke Sprengers, Martin H Koldijk, Els C Brinkman-van der Linden, Laura A Peferoen, Heng Zhang, Wenli Yu, Xinyi Li, Michelle Wagner, Veronica Moreno, Julie Kim, Martha Costa, Kiana West, Zara Fulton, Lucy Chammas, Nancy Luckashenak, Lauren Fletcher, Trevin Holland, Carrie Arnold, R Anthony Williamson, Jeroen J Hoozemans, Adrian Apetri, Frederique Bard, Ian A Wilson, Wouter Koudstaal, Jaap Goudsmit
Several reports have described the presence of antibodies against Alzheimer's disease-associated hyperphosphorylated forms of tau in serum of healthy individuals. To characterize the specificities that can be found, we interrogated peripheral IgG(+) memory B cells from asymptomatic blood donors for reactivity to a panel of phosphorylated tau peptides using a single-cell screening assay. Antibody sequences were recovered, cloned, and expressed as full-length IgGs. In total, 52 somatically mutated tau-binding antibodies were identified, corresponding to 35 unique clonal families...
May 2017: Acta Neuropathologica
https://www.readbyqxmd.com/read/28340818/use-of-atg-fresenius-as-an-induction-agent-in-deceased-donor-kidney-transplantation
#14
M Yilmaz, T Ö Sezer, O Kir, A Öztürk, C Hoşcoşkun, H Töz
BACKGROUND: Anti-T-lymphocyte globulins (ATG) are most commonly used as induction agents in kidney transplantation (KT). In this study, we investigated the use of ATG as induction therapy in deceased-donor KT. METHODS: Among 152 deceased-donor KT recipients transplanted between January 2009 and December 2003, 147 with exact data were enrolled in this study. Delayed graft function was defined as dialysis requirement after KT. Greater than 10% panel-reactive antibody (PRA) was considered as positive...
April 2017: Transplantation Proceedings
https://www.readbyqxmd.com/read/28340817/efficacy-and-safety-of-atg-fresenius-as-an-induction-agent-in-living-donor-kidney-transplantation
#15
M Yilmaz, T Ö Sezer, E Günay, I Solak, A Çeltik, C Hoşcoşkun, H Töz
BACKGROUND: Induction therapy is mostly recommended for deceased-donor transplantation, whereas it has some controversies in live-donor transplantation. In this study, we described the outcomes of live-donor renal transplant recipients who received ATG-Fresenius (ATG-F) induction. METHODS: Live-donor transplantations in patients over 18 years old with ATG-F induction between 2009 and 2015 were included. All patients received quadruple immunosuppression, one of which was ATG-F induction...
April 2017: Transplantation Proceedings
https://www.readbyqxmd.com/read/28340813/comparison-of-anti-hla-antibody-production-according-to-gestational-periods-in-pregnant-women
#16
B Cerci Gurbuz, M Soyoz, D Ozkale Okyay, T Kilicaslan Ayna, I Pirim
BACKGROUND: To evaluate paternal anti-HLA antibody profiles, sera samples were collected from pregnant women in different trimesters and the panel-reactive antibody (PRA) specificities were identified. METHODS: From 2013 to 2015, serum samples were obtained from 41 pregnant women who had registered at the Izmir Tepecik Education and Research Hospital Gynecology Clinic. Anti-HLA antibodies were screened by using the panel reactive antibody screening and identification tests...
April 2017: Transplantation Proceedings
https://www.readbyqxmd.com/read/28340809/corrected-panel-reactive-antibody-positivity-rates-for-hypersensitized-patients-in-turkish-population-with-calculated-panel-reactive-antibody-software
#17
S T Karadeniz, S U Akgul, Y Ogret, H S Ciftci, A Bayraktar, H Bakkaloglu, Y Caliskan, K Yelekci, A Turkmen, A E Aydin, F S Oguz, M Carin, F Aydin
INTRODUCTION: High rates of panel-reactive antibody (PRA) may decrease the chance of kidney transplantation and may result in long waiting periods before transplantation. The calculated PRA (cPRA) is performed based on unacceptable HLA antigens. These antigens are identified by a program that was created based on the antibodies that developed against the HLA antigens circulating in serum and on the risk of binding of these antibodies to antigens. The antigen profile of the population and antigen frequencies can be measured, and more realistic cPRA positivity rates may be obtained using this method...
April 2017: Transplantation Proceedings
https://www.readbyqxmd.com/read/28340805/association-between-hla-antibodies-and-different-sensitization-events-in-renal-transplant-candidates
#18
S U Akgul, H S Ciftci, S Temurhan, Y Caliskan, A Bayraktar, T Tefik, I A Kaya, I O Canitez, E Demir, H Yazici, H Bakkaloglu, A E Aydin, A Turkmen, I Nane, F Aydin, F S Oguz
BACKGROUND: Human leukocyte antigen (HLA) allo-immunization is caused by various events such as blood transfusions, pregnancies, or organ transplantations, which can lead to sensitization. In this retrospective study, we evaluated different sensitization models and their effects on panel-reactive antibody (PRA) profiles of renal transplantation candidates. METHODS: Anti-HLA class I/II antibody screening tests were performed in 906 renal transplantation candidates with the use of a microbead-based assay (Luminex)...
April 2017: Transplantation Proceedings
https://www.readbyqxmd.com/read/28340245/unacceptable-human-leucocyte-antigens-for-organ-offers-in-the-era-of-organ-shortage-influence-on-waiting-time-before-kidney-transplantation
#19
Malte Ziemann, Nicole Heßler, Inke R König, Nils Lachmann, Andrea Dick, Vanessa Ditt, Klemens Budde, Petra Reinke, Ute Eisenberger, Barbara Suwelack, Thomas Klein, Timm H Westhoff, Wolfgang Arns, Katrin Ivens, Antje Habicht, Lutz Renders, Dirk Stippel, Dominik Bös, Florian Sommer, Siegfried Görg, Martin Nitschke, Thorsten Feldkamp, Falko M Heinemann, Reinhard Kelsch
Background.: The assignment of human leucocyte antigens (HLAs) against which antibodies are detected as unacceptable antigens (UAGs) avoids allocation of HLA- incompatible allografts. There is uncertainty as to what extent UAGs decrease the probability of receiving a kidney offer. Methods.: Kidney transplantations in 3264 patients on the waiting lists of six German transplant centres were evaluated for a period of at least 2 years. The proportion of excluded offers due to UAGs was calculated as virtual panel-reactive antibodies (vPRAs)...
March 15, 2017: Nephrology, Dialysis, Transplantation
https://www.readbyqxmd.com/read/28330741/intermediate-steroid-withdrawal-after-renal-transplantation-and-anti-hla-antibodies-hla-abs-development
#20
Elena Monfá, David San Segundo, Juan Carlos Ruiz San Millán, Judith Sanabria, Zoila Albines, Emilio Rodrigo, Iñigo Romón, Esther Asensio, Manuel Arias, Marcos López-Hoyos
INTRODUCTION: Steroid withdrawal in renal transplantation is desirable to avoid their adverse effects. However, by decreasing the immunosuppression, could lead to an increased risk for the development of HLA-Abs. OBJECTIVE: Evaluate the relationship between steroid withdrawal and development of HLA-Abs in renal transplantation. METHODS: We analyzed sera by Luminex from 182 kidney transplants performed from 1998 to 2011, before and two years after transplantation...
March 19, 2017: Nefrología: Publicación Oficial de la Sociedad Española Nefrologia
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