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https://www.readbyqxmd.com/read/29143045/long-wavelength-fluorescent-boronate-probes-for-the-detection-and-intracellular-imaging-of-peroxynitrite
#1
Adam C Sedgwick, Hai-Hao Han, Jordan E Gardiner, Steven D Bull, Xiao-Peng He, Tony D James
Two boronate fluorescent probes have been developed for the detection of peroxynitrite (TCFB1 and TCFB2). TCFB1 was shown to have a low sensitvity towards peroxynitrite and have a poor solubility in aqueous solution whereas TCFB2 demonstrated high sensitivity towards peroxynitrite and mitochondria localisation with the ability to detect exogenous and endogenous peroxynitrite in live cells (Hep-G2, RAW 264.7, HeLa and A459).
November 16, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/29142410/methanolic-extract-of-costus-pictus-d-don-induces-cytotoxicity-in-liver-hepatocellular-carcinoma-cells-mediated-by-histone-deacetylase-inhibition
#2
P V Neethu, K Suthindhiran, M A Jayasri
Background: Leaves of Costus pictus D. Don, (insulin plant) are used as dietary supplement for the treatment of diabetes. Objective: The antidiabetic activity of this plant is well documented, but its activity on different cell types and mechanism remains unknown. Thus, the present study evaluates the cytotoxicity of C. pictus methanolic extract (CPME) against various cancer and normal cells. Materials and Methods: Dried leaves of C. pictus were extracted using methanol and were subjected to histone deacetylase (HDAC) inhibition and toxicity studies...
October 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/29133054/synthesis-crystal-structure-and-biological-evaluation-of-a-new-dasatinib-copper-ii-complex-as-telomerase-inhibitor
#3
Qi-Pin Qin, Ting Meng, Ming-Xiong Tan, Yan-Cheng Liu, Xu-Jian Luo, Bi-Qun Zou, Hong Liang
A new copper(II) complex of dasatinib (DAS) was synthesized and characterized via ESI-MS, UV-Vis, IR, single-crystal X-ray diffraction analysis, (1)H and (13)C NMR spectroscopy, and elemental analysis. The composition of the new complex (1) was found to be [Cu(DAS + H)(NO3)2(H2O)]NO3·(H2O)·(CH3OH). Through MTT assay, it was found that 1 had high cytotoxicity towards A549, HeLa, BEL-7402, Hep-G2, NCI-H460, and MGC80-3 tumor cell lines, with IC50 values in 4.04-13.04 μM. The Hep-G2 cells were the most sensitive to 1...
October 25, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29126737/synthesis-and-in%C3%A2-vitro-biological-evaluation-of-three-4-4-methoxyphenyl-2-2-6-2%C3%A2-terpyridine-iridium-iii-complexes-as-new-telomerase-inhibitors
#4
Qi-Pin Qin, Ting Meng, Ming-Xiong Tan, Yan-Cheng Liu, Xu-Jian Luo, Bi-Qun Zou, Hong Liang
There iridium(III) complexes, [Ir(3-MeO-Phtpy)Cl3] (1), [Ir(2-MeO-Phtpy)Cl3] (2) and [Ir(4-MeO-Phtpy)Cl3] (3) with 4'-(3-methoxyphenyl)-2,2':6',2″-terpyridine (3-MeO-Phtpy), 4'-(2-methoxyphenyl)-2,2':6',2″-terpyridine (2-MeO-Phtpy) and 4'-(4-methoxyphenyl)-2,2':6',2″-terpyridine (4-MeO-Phtpy) as ligands, respectively, were synthesized and evaluated for their antiproliferative activities. In these complexes, the iridium(III) center adopts a six-coordinate distorted octahedral geometry. Among them, complex 1 exhibited the most potent activity, with IC50 values of 3...
October 16, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29108284/overexpression-of-mir-26b-decreases-the-cisplatin-resistance-in-laryngeal-cancer-by-targeting-atf2
#5
Linli Tian, Jiarui Zhang, Xiuxia Ren, Xinyu Liu, Wei Gao, Chen Zhang, Yanan Sun, Ming Liu
Cisplatin is a common used chemotherapeutic drug for the treatment of laryngeal cancer. However, drug-resistance is a major obstacle in platinum-based chemotherapy for laryngeal cancer. Recent studies have demonstrated that dysregulation of microRNAs (miRNAs) is responsible for chemoresistance in multiple cancers including laryngeal cancer, but the potential mechanisms are required to be explored. In the present study, we constantly exposed the laryngeal cancer cell line Hep-2 with cisplatin to establish a cisplatin-resistant laryngeal cancer cell model (Hep-2/R)...
October 3, 2017: Oncotarget
https://www.readbyqxmd.com/read/29097358/lncrna-miat-promoted-proliferation-and-invasion-of-hcc-cells-via-sponging-mir-214
#6
Xinli Huang, Yun Gao, Jianjie Qin, Sen Lu
BACKGROUND: The aberrant expression of lncRNAs has been involved in various human tumors including hepatocellular carcinoma (HCC). Our study aimed to investigate the potential molecular mechanism of lncRNA-MIAT in HCC. METHODS: The expression of MIAT and miR-214 in HCC tissues and cells was examined by qRT-PCR, and the levels of EZH2 and β-catenin were detected by western blot assay. The immunoprecipitation analysis was used to detect the level of H3/H4 histone acetylation...
November 2, 2017: American Journal of Physiology. Gastrointestinal and Liver Physiology
https://www.readbyqxmd.com/read/29089415/periprocedural-heparin-use-in-acute-ischemic-stroke-endovascular-therapy-the-trevo-2-trial
#7
Melanie J Winningham, Diogo C Haussen, Raul G Nogueira, David S Liebeskind, Wade S Smith, Helmi L Lutsep, Tudor G Jovin, Bin Xiang, Fadi Nahab
BACKGROUND: The use of periprocedural heparin has previously been reported to be safe and potentially beneficial during thrombectomy with older generation devices. We aimed to evaluate the safety and clinical outcomes of heparin use in the stent retriever era. METHODS: A post hoc analysis of the TREVO 2 trial was performed comparing baseline characteristics and clinical outcomes between patients who received (HEP+) and those who did not receive periprocedural heparin (HEP-) while undergoing MERCI or TREVO clot retrieval...
October 31, 2017: Journal of Neurointerventional Surgery
https://www.readbyqxmd.com/read/29075151/sporadic-pcdh18-somatic-mutations-in-epcam-positive-hepatocellular-carcinoma
#8
Takehiro Hayashi, Taro Yamashita, Hikari Okada, Kouki Nio, Yasumasa Hara, Yoshimoto Nomura, Tomoyuki Hayashi, Yoshiro Asahina, Mariko Yoshida, Naoki Oishi, Hajime Sunagozaka, Hajime Takatori, Masao Honda, Shuichi Kaneko
BACKGROUND: The relationship between specific genome alterations and hepatocellular carcinoma (HCC) cancer stem cells (CSCs) remains unclear. In this study, we evaluated the relationship between somatic mutations and epithelial cell adhesion molecule positive (EpCAM(+)) CSCs. METHODS: Two patient-derived HCC samples (HCC1 and HCC2) were sorted by EpCAM expression and analyzed by whole exome sequence. We measured PCDH18 expression level in eight HCC cell lines as well as HCC1 and HCC2 by real-time quantitative RT-PCR...
2017: Cancer Cell International
https://www.readbyqxmd.com/read/29066296/two-novel-bioactive-sulfated-guaiane-sesquiterpenoid-salt-alkaloids-from-the-aerial-parts-of-scorzonera-divaricata
#9
Quan-Xiang Wu, Xiao-Feng He, Chun-Xiao Jiang, Wei Zhang, Zhuan-Ning Shi, Hong-Fang Li, Ying Zhu
Extracts of the aerial parts of Scorzonera divaricata afforded sulfoscorzonin D (1) and sulfoscorzonin E (2), two novel pyrrolidine inner salt alkaloids with a sulfated guaiane sesquiterpene lactone nucleus, along with 22 known compounds. Especially, sulfoscorzonin D containing a unusual monoterpene moiety is very rare. The structures of new compounds were established using spectroscopic analysis including one- and two-dimensional NMR and HRESIMS. The cytotoxicities of compounds 1-4 and 10 against three tumor cell lines (K562, Hela, and Hep-G2) were evaluated using the MTT assay...
October 21, 2017: Fitoterapia
https://www.readbyqxmd.com/read/29063721/high-yield-purification-and-first-structural-characterization-of-the-full-length-bacterial-toxin-cnf1
#10
Andrea Colarusso, Marco Caterino, Alessia Fabbri, Carla Fiorentini, Alessandro Vergara, Filomena Sica, Ermenegilda Parrilli, Maria Luisa Tutino
The Cytotoxic Necrotizing Factor 1 (CNF1) is a bacterial toxin secreted by certain Escherichia coli strains causing severe pathologies, making it a protein of pivotal interest in toxicology. In parallel, the CNF1 capability to influence important neuronal processes, like neuronal arborisation, astrocytic support, and efficient ATP production, has been efficiently used in the treatment of neurological diseases, making it a promising candidate for therapy. Nonetheless, there are still some unsolved issues about the CNF1 mechanism of action and structuration probably caused by the difficulty to achieve sufficient amounts of the full-length protein for further studies...
October 24, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/29063370/pathways-of-production-and-delivery-of-hepatocyte-exosomes
#11
Li Chen, Ruju Chen, Sherri Kemper, David R Brigstock
Hepatocyte exosomes (Exo(Hep)) are proposed to mediate physiological or pathophysiological signaling in a variety of hepatic target cells. Exo(Hep) were purified from the medium of primary mouse hepatocytes or AML12 cells and characterized as ~100 nm nanovesicles that were positive for proteins commonly found in exosomes (CD9, CD81, flotillin) or hepatocytes (asialoglycoprotein receptor). Ethanol treatment of hepatocytes caused increased Exo(Hep) release and increased cellular mRNA expression of components involved in intracellular vesicle trafficking (Rab 5a,b,c, Rab 7a, Rab 27a,b) or exosome biogenesis via the ESCRT (HGS, Alix, STAM1, TSG101, VTA1, YKT6) or ceramide (nSmase2) pathways...
October 23, 2017: Journal of Cell Communication and Signaling
https://www.readbyqxmd.com/read/29053813/comparative-transcriptomic-analysis-of-high-and-low-egg-producing-duck-ovaries
#12
Z Tao, W Song, C Zhu, W Xu, H Liu, S Zhang, Li Huifang
The egg-laying rate is an important indicator of egg production of laying ducks. Egg production directly impacts the economic benefits of the duck industry. In order to obtain better insight into the molecular mechanisms associated with the process of egg production, comparative transcriptomic analysis of the ovaries of Jinding ducks with high and low egg production was performed using the Illumina HiSeq 2500 system. A total of 843 differentially expressed genes (DEGs) was identified, 367 that were down-regulated and 476 that were up-regulated in high egg production (HEP) ovaries, as compared with low egg production (LEP) ovaries...
October 5, 2017: Poultry Science
https://www.readbyqxmd.com/read/28985385/evaluating-an-indigenous-health-curriculum-for-diabetes-prevention-engaging-the-community-through-talking-circles-and-knowledge-translation-of-results
#13
Maedeh Khayyat Kholghi, Gillian Bartlett, Morgan Phillips, Jon Salsberg, Alex M McComber, Ann C Macaulay
Background: Kahnawà:ke is a Kanien'kehá:ka (Mohawk) community in Quebec, Canada. In 1997, the community-controlled Kateri Memorial Hospital Centre in partnership with the Kahnawake Education Center, and the Kahnawake Schools Diabetes Prevention Project (KSDPP) developed an elementary school diabetes prevention health education program, aimed to increase knowledge of Type 2 diabetes, healthy eating and active lifestyles. Long-term goals for KSDPP community and school interventions are to decrease obesity and diabetes...
July 24, 2017: Family Practice
https://www.readbyqxmd.com/read/28970539/cytotoxic-serrulatane-type-diterpenoids-from-the-gorgonian-euplexaura-sp-and-their-absolute-configurations-by-vibrational-circular-dichroism
#14
Fei Cao, Chang-Lun Shao, Yun-Feng Liu, Hua-Jie Zhu, Chang-Yun Wang
Vibrational circular dichroism (VCD) method has become robust and reliable alternative for the stereochemical characterization of natural products. In this paper, three new serrulatane-type diterpenoids, euplexaurenes A-C (1-3), and a known metabolite, anthogorgiene P (4), were obtained from the South China Sea gorgonian Euplexaura sp. GXWZ-05. The absolute configuration of C-11 in 1-4, which was difficult to be determined by common means due to the high conformational flexibility of the eight-carbon aliphatic chain attached at C-4, was determined by VCD method, suggesting a new horizon to define the absolute configurations of natural products possessing chains...
October 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28969111/citral-a-monoterpene-protect-against-high-glucose-induced-oxidative-injury-in-hepg2-cell-in-vitro-an-experimental-study
#15
Sri Devi Subramaniyan, Ashok Kumar Natarajan
INTRODUCTION: Diabetes mellitus, a major metabolic disorder associated with hyperglycaemia is one of the leading cause of death in many developed countries. However, use of natural phytochemicals have been proved to have a protective effect against oxidative damage. AIM: To investigate the effect of citral, a monoterpene on high glucose induced cytotoxicity and oxidative stress in human hepatocellular liver carcinoma (Hep G2) cell line. MATERIALS AND METHODS: Cells were treated with 50 mM concentration of glucose for 24 hours incubation following citral (30 μM) was added to confluent HepG2 cells...
August 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28947219/comparison-between-saline-solution-containing-heparin-versus-saline-solution-in-the-lock-of-totally-implantable-catheters
#16
A R O Brito, K Nishinari, P F Saad, K R Saad, M A T Pereira, S C D Emídio, G Yazbek, G A Z Bomfim, R N Cavalcante, M Krutman, M P Teivelis, B S Pignataro, I Y I Fonseca, G Centofanti, B L F Soares
INTRODUCTION: there are only three studies comparing the efficacy of two different types of lock used in totally implantable catheters regarding occlusion or reflux dysfunction. The present study contains the largest published casuistry (862 patients) and is the only that analyzes three parameters: occlusion, reflux dysfunction and flow dysfunction. METHOD: it was a retrospective study of patients operated at a large oncology center and followed up in the outpatient clinic between 2007 and 2015...
September 22, 2017: Annals of Vascular Surgery
https://www.readbyqxmd.com/read/28946185/cytotoxic-effect-of-cyperus-rotundus-rhizome-extract-on-human-cancer-cell-lines
#17
Prabu Mannarreddy, Maghil Denis, Durgadevi Munireddy, Ranjani Pandurangan, Kalaichelvan Puthupalayam Thangavelu, Kaviyarasan Venkatesan
The wild weed Cyperus rotundus is commonly used as traditional medicine in different parts of the world. Sequential extraction of C. rotundus rhizome with solvents of different polarity namely hexane, chloroform, ethyl acetate, methanol and water were prepared and the free radical scavenging activity was determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. Based on high antioxidant activity of methanolic extract of C. rotundus rhizome (MRCr) was further investigated for its cytotoxic effect on different human cancer cell lines-breast (MCF-7), cervical (HeLa), liver (Hep G2), prostate (PC-3), colorectal (HT-29) and normal cell line (MCF-12A) by 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay evaluated as 50% inhibition of growth (IC50)...
November 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28937364/nucleic-acid-amplification-testing-in-indian-blood-banks-a-review-with-perspectives
#18
Kanjaksha Ghosh, Kanchan Mishra
BACKGROUND: Nucleic acid amplification testing (NAT) is restricted to a few blood banks in India since 2008. This review was directed toward understanding NAT yield in different parts of the country and prevalence in the NAT of different types of virus. MATERIALS AND METHODS: English literature was searched from 1990 to 2016 in PubMed, Scopus, Ind med, and Google database using properly constructed key words. Literature was collected and finally the data were synthesized...
July 2017: Indian Journal of Pathology & Microbiology
https://www.readbyqxmd.com/read/28928894/mzf-1-elk-1-pkc%C3%AE-is-associated-with-poor-prognosis-in-patients-with-hepatocellular-carcinoma
#19
Je-Chiuan Ye, Li-Sung Hsu, Jen-Hsiang Tsai, Hsin-Ling Yang, Meen-Woon Hsiao, Jin-Ming Hwang, Chia-Jen Lee, Jer-Yuh Liu
Background: Protein kinase C alpha (PKCα) is a key signaling molecule in human cancer development. As a therapeutic strategy, targeting PKCα is difficult because the molecule is ubiquitously expressed in non-malignant cells. PKCα is regulated by the cooperative interaction of the transcription factors myeloid zinc finger 1 (MZF-1) and Ets-like protein-1 (Elk-1) in human cancer cells. Methods: By conducting tissue array analysis, herein, we determined the protein expression of MZF-1/Elk-1/PKCα in various cancers...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/28928726/phosphoprotein-gene-contributes-to-the-enhanced-apoptosis-induced-by-wild-type-rabies-virus-gd-sh-01-in-vitro
#20
Qin Tian, Yifei Wang, Qiong Zhang, Jun Luo, He Jiang, Boyue Zhang, Mingzhu Mei, Fan Wu, Yuting Wu, Jiaojiao Peng, Teng Long, Yongwen Luo, Xiaofeng Guo
Previous research demonstrated that the matrix protein (M) and glycoprotein (G) of attenuated rabies virus (RABV) strains are involved in the induction of host cell apoptosis. In this work, we show that wild-type (wt) RABV GD-SH-01 induces significantly greater apoptosis than the attenuated strain HEP-Flury. In order to identify the gene(s) accounting for this phenotype, five recombinant RABVs (rRABVs) were constructed by replacing each single gene of HEP-Flury with the corresponding gene of GD-SH-01. By using these rRABVs, we found that not only M and G, but also the phosphoprotein (P) plays an important role in inducing apoptosis...
2017: Frontiers in Microbiology
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