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https://www.readbyqxmd.com/read/28789962/expression-and-characterization-of-an-enhanced-recombinant-heparinase-i-with-chitin-binding-domain
#1
Shuqin Xu, Meiling Qiu, Xuanyue Zhang, Jinghua Chen
Heparinase I (Hep I) can efficiently depolymerize heparin and heparin sulfate to oligosaccharides or unsaturated disaccharides, which resulted in loss of physiological function such as blood coagulation. In order to realize the immobilization of Hep I on chitin carriers, we cloned Hep I with the chitin binding domain (ChBD) as a chitin-affinity tag, and the Small Ubiquitin-like MOdifier (SUMO) linker as a solvation enhancer in different fusion sequence. DNA and protein gels suggested that 4 kinds of recombinants were successfully constructed and expressed in Escherichia coli (E...
August 5, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28778116/caveolin-1-restricts-group-a-streptococcus-invasion-of-non-phagocytic-host-cells
#2
Jin Yan Lim, Timothy C Barnett, Michele Bastiani, Kerrie-Ann McMahon, Charles Ferguson, Richard I Webb, Robert G Parton, Mark J Walker
Caveolae are composed of two major proteins, caveolin-1 (CAV1) and cavin-1/polymerase transcript factor I (CAVIN1). Here we demonstrate that CAV1 levels modulate invasion of group A Streptococcus (GAS) into non-phagocytic mammalian cells. GAS showed enhanced internalization into CAV1 knockout mouse embryonic fibroblasts (MEFs), and CAV1 knockdown human epithelial HEp-2 cells, whereas over-expression of CAV1 in HEp-2 cells reduced GAS invasion. This effect was not dependent on the expression of the GAS fibronectin binding protein SfbI, which had previously been implicated in caveolae-mediated uptake...
August 4, 2017: Cellular Microbiology
https://www.readbyqxmd.com/read/28770960/expression-of-mir-195-in-laryngeal-squamous-cell-carcinoma-and-its-effect-on-proliferation-and-apoptosis-of-hep-2
#3
Y Shuang, C Li, X Zhou, Y-W Huang, L Zhang
OBJECTIVE: To investigate the expression of miR-195 and its relationship with clinicopathological characteristics in laryngeal squamous cell carcinoma (LSCC), and to explore its effect and possible mechanism on proliferation and apoptosis of Hep-2. PATIENTS AND METHODS: Real-time fluorescence quantitative PCR was used to detect the expression of miR-195 in laryngeal carcinoma tissues and adjacent normal tissues from 98 cases. Dual-luciferase reporter plasmid with Bcl-2 wild type and mutant type 3' untranslated region was created to verify the target of miR-195 by luciferase assay...
July 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28756153/hybrid-pharmacophore-approach-for-bio-relevant-di-imines-based-homobimetallic-complexes-incorporating-functionalized-dicarboxylates-as-co-ligands-synthesis-spectral-and-structural-activity-dependent-biological-insights-in-vitro-dna-and-hsa-binding-antioxidant
#4
Mohammad Shakir, Summaiya Hanif, Md Fazle Alam, Mohd Farhan, Hina Younus
Synthesis of bio-efficient homobimetallic complexes, [Cu2(L(1))2(dipic)](NO3)2.3H2O (1), [Zn2(L(1))2(dipic)](NO3)2.4H2O (2), [Cu2(L(2))2(oxa)](NO3)2.4H2O (3) and [Zn2(L(2))2(oxa)] (NO3)2.5H2O (4) was carried out using Schiff bases [(N(1)E,N(2)E)-N(1),N(2)-bis(5-chlorothiophen-2-ylmethylene)-4-chlorobenzene-1,2-diamine; L(1)] and [(N(1)E,N(2)E)-N(1),N(2)-bis(5-chlorofuran-2-ylmethylene)-4-chlorobenzene-1,2-diamine; L(2)] as main ligands and dicarboxylate moieties of 2,6-pyridine dicarboxylic acid (H2-dipic) and oxalic acid (H2-oxa) as co-ligands, respectively in order to apprehend their structure activity relationships on the basis of pharmacophore hybrid approach...
July 24, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28755871/cyclohexene-fused-1-3-oxazines-with-selective-antibacterial-and-antiparasitic-action-and-low-cytotoxic-effects
#5
Maria R M de Brito, Walter J Peláez, Martín S Faillace, Gardenia C G Militão, Jackson R G S Almeida, Gustavo A Argüello, Zsolt Szakonyi, Ferenc Fülöp, Maria C Salvadori, Fernanda S Teixeira, Rivelilson M Freitas, Pedro L S Pinto, Ana C Mengarda, Marcos P N Silva, Ademar A Da Silva Filho, Josué de Moraes
Oxazine derivatives, a class of heterocyclic compounds, exhibit a variety of biological properties, such as anticonvulsant and antitumor activities. In this study, we evaluated the effect of two cyclohexene-fused 1,3-oxazines (cis‑1-benzyl-N-phenyl-1,4,4a,5,8,8a-hexahydro-3,1-benzoxazin-2-imine (1) and trans‑N-phenyl-1,4,4a,5,8,8a-hexahydro-3,1-benzoxazin-2-imine (2)) in cultures of Bacillus cereus, Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae, Salmonella enterica, Serratia marcescens, Shigella flexneri and Staphylococcus aureus by the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC)...
July 26, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28754534/health-related-quality-of-life-during-trans-arterial-chemoembolization-with-drug-eluting-beads-loaded-with-doxorubicin-debdox-for-unresectable-hepatic-metastases-from-ocular-melanoma
#6
Jack W Rostas, Alda L Tam, Takami Sato, Charles R Scoggins, Kelly M McMasters, Robert C G Martin
BACKGROUND: We have previously reported favorable response and survival rates using drug-eluting beads loaded with doxorubicin (DEBDOX) for unresectable hepatic metastases. This study investigates the quality of life (QoL) impact of DEBDOX for the treatment of unresectable hepatic metastases from melanoma. METHODS: A multi-center, prospective, non-controlled clinical trial was reviewed. QoL was assessed at baseline and after each treatment, and doxorubicin-specific effects were assessed after each treatment...
July 21, 2017: American Journal of Surgery
https://www.readbyqxmd.com/read/28737690/synthesis-physico-chemical-characterization-crystal-structure-and-influence-on-microbial-and-tumor-cells-of-some-co-ii-complexes-with-5-7-dimethyl-1-2-4-triazolo-1-5-a-pyrimidine
#7
Luminiţa Măruţescu, Larisa Calu, Mariana Carmen Chifiriuc, Coralia Bleotu, Constantin-Gabriel Daniliuc, Denisa Fălcescu, Crina Maria Kamerzan, Mihaela Badea, Rodica Olar
Three complexes, namely [Co(dmtp)₂(OH₂)₄][CoCl₄] (1), [Co(dmtp)₂Cl₂] (2) and [Co(dmtp)₂(OH₂)₄]Cl₂∙2H₂O (3) (dmtp: 5,7-dimethyl-1,2,4-triazolo[1,5-a]pyrimidine), were synthesized and characterized by spectral (IR, UV-Vis-NIR), and magnetic measurements at room temperature, as well as single crystal X-ray diffraction. Complex (1) crystallizes in monoclinic system (space group C2/c), complex (2) adopts an orthorhombic system (space group Pbca), and complex (3) crystallizes in triclinic system (space group P1)...
July 22, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28727444/fosfomycin-biosynthesis-via-transient-cytidylylation-of-2-hydroxyethylphosphonate-by-the-bifunctional-fom1-enzyme
#8
Su-Hee Cho, Seung-Young Kim, Takeo Tomita, Taro Shiraishi, Jin-Soo Park, Shusuke Sato, Fumitaka Kudo, Tadashi Eguchi, Nobutaka Funa, Makoto Nishiyama, Tomohisa Kuzuyama
Fosfomycin is a wide-spectrum phosphonate antibiotic that is used clinically to treat cystitis, tympanitis, etc. Its biosynthesis starts with the formation of a carbon-phosphorus bond catalyzed by the phosphoenolpyruvate phosphomutase Fom1. We identified an additional cytidylyltransferase (CyTase) domain at the Fom1 N-terminus in addition to the phosphoenolpyruvate phosphomutase domain at the Fom1 C-terminus. Here, we demonstrate that Fom1 is bifunctional and that the Fom1 CyTase domain catalyzes the cytidylylation of the 2-hydroxyethylphosphonate (HEP) intermediate to produce cytidylyl-HEP...
July 20, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/28720432/adhesion-and-invasion-of-clostridium-perfringens-type-a-into-epithelial-cells
#9
Luis A Llanco, Viviane Nakano, Claudia T P de Moraes, Roxane M F Piazza, Mario J Avila-Campos
Clostridium perfringens is the causative agent for necrotic enteritis. It secretes the major virulence factors, and α- and NetB-toxins that are responsible for intestinal lesions. The TpeL toxin affects cell morphology by producing myonecrosis, but its role in the pathogenesis of necrotic enteritis is unclear. In this study, the presence of netB and tpeL genes in C. perfringens type A strains isolated from chickens with necrotic enteritis, their cytotoxic effects and role in adhesion and invasion of epithelial cells were evaluated...
July 7, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28706487/pekinenin-e-inhibits-the-growth-of-hepatocellular-carcinoma-by-promoting-endoplasmic-reticulum-stress-mediated-cell-death
#10
Lu Fan, Qingling Xiao, Yanyan Chen, Gang Chen, Jinao Duan, Weiwei Tao
Hepatocellular carcinoma (HCC) is a malignant primary liver cancer with poor prognosis. In the present study, we report that pekinenin E (PE), a casbane diterpenoid derived from the roots of Euphorbia pekinensis, has a strong antitumor activity against human HCC cells both in vitro and in vivo. PE suppressed the growth of human HCC cells Hep G2 and SMMC-7721. In addition, PE-mediated endoplasmic reticulum (ER) stress caused increasing expressions of C/EBP homologous protein (CHOP), leading to apoptosis in HCC cells both in vitro and in vivo...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28701716/glyat-regulates-jnk-mediated-cell-death-in-drosophila
#11
Pu Ren, Wenzhe Li, Lei Xue
Cell death is a fundamental progress that regulates cell number, tissue homeostasis and organ size in development. The c-Jun N-terminal kinase (JNK) pathway has been evolutionarily conserved from fly to human, and plays essential roles in regulating cell death. To characterize additional genes that regulate JNK signaling, we performed a genetic screen in Drosophila and identified dGLYAT, a novel gene whose function was previously unknown, as a modulator of JNK-mediated cell death. We found that loss of dGLYAT suppressed JNK activation and cell death triggered by over-expression of Egr or Hep, or depletion of puc or lgl in development, suggesting dGLYAT regulates both ectopic and physiological functions of JNK pathway...
July 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28689856/restrictions-on-access-to-direct-acting-antivirals-for-people-who-inject-drugs-the-european-hep-core-study-and-the-role-of-patient-groups-in-monitoring-national-hcv-responses
#12
J V Lazarus, K Safreed-Harmon, S R Stumo, M Jauffret-Roustide, M Maticic, T Reic, E Schatz, J Tallada, M Harris
In 2016, the World Health Organization (WHO) called for the elimination of viral hepatitis as a public health threat and established the targets of achieving an 80% reduction in new infections and a 65% reduction in deaths from the hepatitis C virus (HCV) by 2030. If European countries are to come close to reaching the WHO targets, they must squarely address the HCV prevention, testing and treatment needs of people who inject drugs (PWID). This viewpoint reports on findings from the 2016 European Hep-CORE study, which was unique in its utilisation of patient groups as a source of hepatitis policy information...
July 6, 2017: International Journal on Drug Policy
https://www.readbyqxmd.com/read/28684446/persistence-of-exhaustion-in-cured-hep-c
#13
David E Kaplan
No abstract text is available yet for this article.
July 6, 2017: Blood
https://www.readbyqxmd.com/read/28678963/scientometric-indicators-for-brazilian-research-on-high-energy-physics-1983-2013
#14
Gonzalo R Alvarez, Samile A S Vanz, Marcia C Barbosa
This article presents an analysis of Brazilian research on High Energy Physics (HEP) indexed by Web of Science (WoS) from 1983 to 2013. Scientometric indicators for output, collaboration and impact were used to characterize the field under study. The results show that the Brazilian articles account for 3% of total HEP research worldwide and that the sharp rise in the scientific activity between 2009 and 2013 may have resulted from the consolidation of graduate programs, the increase of the funding and of the international collaboration as well as the implementation of the Rede Nacional de Física de Altas Energias (RENAFAE) in 2008...
June 29, 2017: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/28678474/methylcobalamin-dependent-radical-sam-c-methyltransferase-fom3-recognizes-cytidylyl-2-hydroxyethylphosphonate-and-catalyzes-the-nonstereoselective-c-methylation-in-fosfomycin-biosynthesis
#15
Shusuke Sato, Fumitaka Kudo, Seung-Young Kim, Tomohisa Kuzuyama, Tadashi Eguchi
A methylcobalamin (MeCbl)-dependent radical S-adenosyl-l-methionine (SAM) methyltransferase Fom3 was found to catalyze the C-methylation of cytidylyl-2-hydroxyethylphosphonate (HEP-CMP) to give cytidylyl-2-hydroxypropylphosphonate (HPP-CMP), although it was originally proposed to catalyze the C-methylation of 2-hydroxyethylphosphonate to give 2-hydroxypropylphosphonate in the biosynthesis of a unique C-P bond containing antibiotic fosfomycin in Streptomyces. Unexpectedly, the Fom3 reaction product from HEP-CMP was almost a 1:1 diastereomeric mixture of HPP-CMP, indicating that the C-methylation is not stereoselective...
July 18, 2017: Biochemistry
https://www.readbyqxmd.com/read/28672193/quantitative-differentiation-of-multiple-virus-in-blood-using-nanoporous-silicon-oxide-immunosensor-and-artificial-neural-network
#16
W Chakraborty, R Ray, N Samanta, C RoyChaudhuri
In spite of the rapid developments in various nanosensor technologies, it still remains challenging to realize a reliable ultrasensitive electrical biosensing platform which will be able to detect multiple viruses in blood simultaneously with a fairly high reproducibility without using secondary labels. In this paper, we have reported quantitative differentiation of Hep-B and Hep-C viruses in blood using nanoporous silicon oxide immunosensor array and artificial neural network (ANN). The peak frequency output (fp) from the steady state sensitivity characteristics and the first cut off frequency (fc) from the transient characteristics have been considered as inputs to the multilayer ANN...
June 24, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28662680/systematic-review-of-patient-reported-quality-of-life-following-stereotactic-ablative-radiotherapy-for-primary-and-metastatic-liver-cancer
#17
REVIEW
Adam Mutsaers, Jeffrey Greenspoon, Cindy Walker-Dilks, Anand Swaminath
BACKGROUND: Stereotactic ablative radiotherapy (SABR) is a safe and effective modality in patients with liver cancer who are ineligible for other local therapies. However SABR is not current standard of practice and requires further validation. Patient reported quality of life (QOL) is key to this validation, yet no systematic reviews to date have been performed to analyse QOL following liver SABR. QOL is a critical part of therapy evaluation, particularly in disease states with short life expectancy...
June 29, 2017: Radiation Oncology
https://www.readbyqxmd.com/read/28652242/preclinical-characterization-of-the-inhaled-small-molecule-respiratory-syncytial-virus-l-protein-polymerase-inhibitor-pc786
#18
Matthew Coates, Daniel Brookes, Young-In Kim, Heather Allen, Euan A F Fordyce, Elizabeth A Meals, Thomas Colley, Claire-Lise Ciana, Guillaume F Parra, Vladimir Sherbukhin, Jennifer A Stockwell, Jennifer C Thomas, S Fraser Hunt, Lauren Anderson-Dring, Stuart T Onions, Lindsey Cass, Peter J Murray, Kazuhiro Ito, Pete Strong, John P Devincenzo, Garth Rapeport
Although respiratory syncytial virus (RSV) is the most common cause of lower respiratory tract infection in infants and young children, attempts to develop an effective therapy have so far proved unsuccessful. Herein we report the preclinical profiles of PC786, a potent non-nucleoside RSV L-protein polymerase inhibitor, designed for inhalation treatment of RSV infection. PC786 demonstrated a potent and selective anti-viral activity against laboratory adapted or clinical isolates of RSVA (IC50: <0.09 - 0...
June 26, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28650400/high-need-to-switch-cart-or-co-medication-with-the-initiation-of-daas-in-elderly-hiv-hcv-co-infected-patients
#19
Elise J Smolders, Colette Smit, Clara Tmm De Kanter, Anton Dofferhoff, Joop E Arends, Kees Brinkman, Bart Rijnders, Marc Van Der Valk, Peter Reiss, David M Burger
BACKGROUD: To describe the use of non-antiretroviral co-medication and combination antiretroviral therapy (cART) in HIV/hepatitis C virus (HCV) co-infected patients, and to predict the potential for drug-drug interactions (DDIs) with direct-acting antivirals (DAAs) against HCV. METHODS: This is a retrospective, cross-sectional study, using the Dutch nationwide ATHENA observational HIV cohort database. All patients with a known HIV/HCV co-infection on 1 January 2015 were included...
June 22, 2017: Journal of Acquired Immune Deficiency Syndromes: JAIDS
https://www.readbyqxmd.com/read/28640349/use-of-gc-ms-to-identify-chemical-constituents-and-cytotoxic-activity-of-the-leaves-of-phoradendron-mucronatum-and-phoradendron-microphyllum-viscaceae
#20
Isla V G A Bastos, Tatiane B DE Oliveira, Maria D Rodrigues, Gardênia C G Militão, Teresinha G DA Silva, Izabel C C Turatti, Norberto P Lopes, Sebastião J DE Melo
Phoradendron mucronatum and P. microphyllum are plants that found in tropical and subtropical areas, used in traditional medicine and popularly known as mistle-thrush. The aim of this study was to identify the chemical constituents of different leaf extracts from P. mucronatum and P. microphyllum and assess cytotoxic activity against strains from a human tumour cells. Extracts obtained with hexane, dichloromethane, chloroform and ethyl acetate from the leaves were analysed by gas chromatography coupled with mass spectrometry (GC-MS) and the cytotoxicity was assessed by the MTT method (bromide (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide))...
April 2017: Anais da Academia Brasileira de Ciências
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