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https://www.readbyqxmd.com/read/29782973/enhanced-retinal-pigment-epithelium-rpe-regeneration-using-curcumin-alginate-hydrogels-in-vitro-evaluation
#1
Jong Ho Park, Eun Yeong Shin, Myeong Eun Shin, Min Joung Choi, Cristiano Carlomagno, Jeong Eun Song, Gilson Khang
The retinal pigment epithelium (RPE) plays a significant role in retaining structural integrity of eye. Factors such as reduction in cell regeneration due to aging and physical injury pose a major hurdle in RPE regeneration. In this study, we exploited the use of alginate (AGT) incorporated with Curcumin (CCI) forming a hydrogel based system CCI/AGT. The fabricated hydrogel could anchor RPE cell in it. In vitro cell analysis revealed that the CCI/AGT hydrogel shows good biocompatibility, enhanced cell growth ability and higher ECM formation compared to the pure AGT hydrogel...
May 18, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29782146/multiscale-interfacial-strategy-to-engineer-mixed-metal-oxide-anodes-towards-an-enhanced-cycling-efficiency
#2
Yue Ma, Cheuk-Wai Tai, Shaowen Li, Kristina Edstrom, Bingqing Wei
Interconnected macro/mesoporous structures of mixed metal oxide (MMO) are developed on nickel foam as free-standing anodes for Li-ion batteries. The sustainable production is realized via a wet chemical etching process with bio-friendly chemicals. By means of the divalent iron doping during an in-situ re-crystallization process, the as-developed MMO anodes exhibit the enhanced levels of cycling efficiency. Furthermore, this atomic-scale modification coherently synergies with the encapsulation layer across a micrometer scale...
May 21, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29781806/the-need-for-understanding-and-engaging-the-patient-as-consumer-of-products-developed-by-neural-engineering
#3
Jennifer French, J Luis Lujan, Dawn Bardot, Emily Lauren Graczyk, Allison Hess-Dunning, Ronald J Triolo, Megan Moynahan, Winny Tan, Adeline Zbrzeski
Neural Engineering is a discipline at the intersection of neuroscience, engineering, and clinical care. Recent major efforts by government and industry aimed at bringing forth personalized therapies, increasing the potential of the neural engineering industry for future growth, eg. the National Institutes of Health (NIH) Brain Research through Advancing Innovative Neurotechnologies (BRAIN) Initiative and Stimulating Peripheral Activity to Relieve Conditions (SPARC) Common Fund Program, the Defense Advanced Research Projects Agency (DARPA) Electrical Prescriptions (ElectRx) and Systems-Based Neurotechnology for Emerging Therapies (SUBNETS) Programs, and the GlaxoSmithKline Bioelectric Medicines Initiative...
May 21, 2018: Journal of Neural Engineering
https://www.readbyqxmd.com/read/29781613/rational-design-of-the-first-lead-tin-fluorooxoborates-mb2o3f2-m-pb-sn-containing-flexible-two-dimensional-b6o12f6-%C3%A2-single-layers-with-widely-divergent-second-harmonic-generation-effects
#4
Min Luo, Fei Liang, Yunxia Song, Dan Zhao, Ning Ye, Zheshuai Lin
Molecular engineering design is a productive strategy on atomic-scale to optimize crystal structure and develop new-functional materials. Herein, the first lead/tin fluorooxoborates, MB2O3F2 (M=Pb, Sn), were rationally designed by employing the nonlinear optical crystal Sr2Be2B2O7 (SBBO) as a parent model. Compared with the rigid [Be6B6O15]∞ double layers in SBBO, MB2O3F2 have flexible two-dimensional [B6O12F6]∞ single layer, which not only keeps the NLO-favorable layered structure but also overcomes the structural instability issues of SBBO...
May 21, 2018: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29781355/development-and-biological-validation-of-a-cyclic-stretch-culture-system-for-the-ex-vivo-engineering-of-tendons
#5
Manuela Teresa Raimondi, Matteo Laganà, Claudio Conci, Michele Crestani, Alessia Di Giancamillo, Francesca Gervaso, Daniela Deponti, Federica Boschetti, Michele M Nava, Chiara Scandone, Cinzia Domeneghini, Alessandro Sannino, Giuseppe M Peretti
INTRODUCTION: An innovative approach to the treatment of tendon injury or degeneration is given by engineered grafts, made available through the development of bioreactors that generate tendon tissue in vitro, by replicating in vivo conditions. This work aims at the design of a bioreactor capable of applying a stimulation of cyclic strain on cell constructs to promote the production of bioartificial tissue with mechanical and biochemical properties resembling those of the native tissue...
May 1, 2018: International Journal of Artificial Organs
https://www.readbyqxmd.com/read/29780481/linking-defects-hierarchical-porosity-generation-and-desalination-performance-in-metal-organic-frameworks
#6
Weibin Liang, Lin Li, Jingwei Hou, Nicholas D Shepherd, Thomas D Bennett, Deanna M D'Alessandro, Vicki Chen
Composite membranes with defective metal-organic frameworks (MOFs) connect the emerging fields of MOF topological modification, MOF-polymer interfacial engineering and composite material functionalization. Although defective MOFs can be fabricated via thermal or chemical treatment, the relationship between hierarchical MOF structure and their performance in a polymeric membrane matrix has so far not been investigated. Here we show how a modulator fumarate-based MIL-53(Al) microwave synthesis process results in defective MOFs...
April 14, 2018: Chemical Science
https://www.readbyqxmd.com/read/29780434/engineered-sulfur-resistant-catalyst-system-with-an-assisted-regeneration-strategy-for-lean-burn-methane-combustion
#7
Niko M Kinnunen, Matthew Keenan, Kauko Kallinen, Teuvo Maunula, Mika Suvanto
Catalytic combustion of methane, the main component of natural gas, is a challenge under lean-burn conditions and at low temperatures owing to sulfur poisoning of the Pd-rich catalyst. This paper introduces a more sulfur-resistant catalyst system that can be regenerated during operation. The developed catalyst system lowers the barrier that has restrained the use of liquefied natural gas as a fuel in energy production.
April 9, 2018: ChemCatChem
https://www.readbyqxmd.com/read/29780215/expression-of-a-rice-soluble-starch-synthase-gene-in-transgenic-wheat-improves-the-grain-yield-under-heat-stress-conditions
#8
Bin Tian, Shyamal K Talukder, Jianming Fu, Allan K Fritz, Harold N Trick
Wheat ( Triticum aestivum L.) is a temperate cereal with an optimum temperature range of 15-22°C during the grain filling stage. Heat stress is one of the major environmental constraints for wheat production worldwide. Temperatures above 25°C during the grain filling stage significantly reduced wheat yield and quality. This reduction was reported due to the inactivation of the soluble starch synthase, a key heat-labile enzyme in starch transformation of wheat endosperm. To improve wheat productivity under heat stress, the rice soluble starch synthase I, under the control of either a constitutive promoter or an endosperm-specific promoter, was expressed in wheat and the transgenic lines were monitored for expression and the effects on yield-related traits...
2018: In Vitro Cellular & Developmental Biology. Plant: Journal of the Tissue Culture Association
https://www.readbyqxmd.com/read/29779717/the-design-and-evaluation-of-an-antimicrobial-resistance-surveillance-system-for-neonatal-intensive-care-units-in-iran
#9
Peyman Rezaei-Hachesu, Taha Samad-Soltani, Sajad Yaghoubi, Marjan GhaziSaeedi, Kayvan Mirnia, Hossein Masoumi-Asl, Reza Safdari
INTRODUCTION: Neonatal intensive care units (NICUs) have complex patients in terms of their diagnoses and required treatments. Antimicrobial treatment is a common therapy for patients in NICUs. To solve problems pertaining to empirical therapy, antimicrobial stewardship programs have recently been introduced. Despite the success of these programs in terms of data collection, there is still inefficiency in terms of analyzing and reporting the data. Thus, to successfully implement these stewardship programs, the design of antimicrobial resistance (AMR) surveillance systems is recommended as a first step...
July 2018: International Journal of Medical Informatics
https://www.readbyqxmd.com/read/29779667/terminal-alkyne-biosynthesis-in-marine-microbes
#10
Xuejun Zhu, Wenjun Zhang
The terminal alkyne is a readily derivatized functionality valued for its diverse applications in material synthesis, pharmaceutical science, and chemical biology. The synthetic biology routes to terminal alkynes are highly desired and yet underexplored. Some marine natural products contain a terminal alkyne functionality, and the discovery of the biosynthetic gene clusters for jamaicamide B and carmabin A marked the beginning of a new era in the understanding and engineering of terminal alkyne biosynthesis...
2018: Methods in Enzymology
https://www.readbyqxmd.com/read/29779478/exosomes-in-therapy-engineering-pharmacokinetic-and-future-applications
#11
Claudia Arenaccio, Chiara Chiozzini, Flavia Ferrantelli, Patrizia Leone, Eleonora Olivetta, Maurizio Federico
BACKGROUND: Eukaryotic cells release vesicles of different sizes under both physiological and pathological conditions. On the basis of the respective biogenesis, extracellular vesicles are classified as apoptotic bodies, microvesicles, and exosomes. Among these, exosomes are considered tools for innovative therapeutic interventions, especially when engineered with effector molecules. The delivery functions of exosomes are favored by a number of typical features. These include their small size (i...
May 20, 2018: Current Drug Targets
https://www.readbyqxmd.com/read/29779229/codeinone-reductase-isoforms-with-differential-stability-efficiency-and-product-selectivity-in-opium-poppy
#12
Mehran Dastmalchi, Limei Chang, Miguel A Torres, Kenneth K S Ng, Peter J Facchini
Codeinone reductase (COR) catalyzes the reversible NADPH-dependent reduction of codeinone to codeine as the penultimate step of morphine biosynthesis in opium poppy (Papaver somniferum). COR also irreversibly reduces neopinone, which forms by spontaneous isomerization in aqueous solution from codeinone, to neopine. In a parallel pathway involving 3-O-desmethylated analogs, COR converts morphinone to morphine, and neomorphinone to neomorphine. Similar to neopine, neomorphine formation by COR is irreversible...
May 19, 2018: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29779127/physiological-and-transcriptomic-analysis-of-a-chronologically-long-lived-saccharomyces-cerevisiae-strain-obtained-by-evolutionary-engineering
#13
Mevlüt Arslan, Can Holyavkin, Halil İbrahim Kısakesen, Alican Topaloğlu, Yusuf Sürmeli, Zeynep Petek Çakar
High-throughput aging studies with yeast as a model organism involve transposon-mutagenesis and yeast knockout collection, which have been pivotal strategies for understanding the complex cellular aging process. In this study, a chronologically long-lived Saccharomyces cerevisiae mutant was successfully obtained by using another high-throughput approach, evolutionary engineering, based on systematic selection in successive batch cultures under gradually increasing levels of caloric restriction. Detailed comparative physiological and transcriptomic analyses of the chronologically long-lived mutant and the reference strain revealed enhanced levels of respiratory metabolism, upregulation of genes related to carbohydrate metabolic processes, glycogen-trehalose pathways, stress response, and repression of protein synthesis-related genes in the long-lived mutant SRM11, already in the absence of caloric restriction...
May 19, 2018: Molecular Biotechnology
https://www.readbyqxmd.com/read/29778216/nitric-oxide-signalling-in-yeast
#14
Rika I Astuti, Ryo Nasuno, Hiroshi Takagi
Nitric oxide (NO) is a cellular signalling molecule widely conserved among organisms, including microorganisms such as bacteria, yeasts, and fungi, and higher eukaryotes such as plants and mammals. NO is mainly produced by the activities of NO synthase (NOS) or nitrite reductase (NIR). There are several NO detoxification systems, including NO dioxygenase (NOD) and S-nitrosoglutathione reductase (GSNOR). NO homeostasis, based on the balance between NO synthesis and degradation, is important for regulating its physiological functions, since an excess of NO causes nitrosative stress due to the high reactivity of NO and NO-derived compounds...
2018: Advances in Microbial Physiology
https://www.readbyqxmd.com/read/29777593/degradation-of-recombinant-proteins-by-cho-host-cell-proteases-is-prevented-by-matriptase-1-knock-out
#15
Holger Laux, Sandrine Romand, Sandro Nuciforo, Christopher J Farady, Joel Tapparel, Stine Buechmann-Moeller, Benjamin Sommer, Edward J Oakeley, Ursula Bodendorf
An increasing number of non-antibody format proteins are entering the clinical development. However, one of the major hurdles for the production of non-antibody glycoproteins is host cell-related proteolytic degradation, which can drastically impact developability and timelines of pipeline projects. Chinese hamster ovary (CHO) cells are the preferred production host for recombinant therapeutic proteins. Using protease inhibitors, transcriptomics and genetic knockdowns we have identified, out of the more than 700 known proteases in rodents, Matriptase-1 as the major protease involved in degradation of recombinant proteins expressed in CHO-K1 cells...
May 19, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29777485/co-suppression-of-atmips-demonstrates-cooperation-of-mips1-mips2-and-mips3-in-maintaining-myo-inositol-synthesis
#16
C M Fleet, J Y Yen, E A Hill, G E Gillaspy
Co-suppressed MIPS2 transgenic lines allow bypass of the embryo lethal phenotype of the previously published triple knock-out and demonstrate the effects of MIPS on later stages of development. Regulation of inositol production is of interest broadly for its effects on plant growth and development. The enzyme L-myo-inositol 1-phosphate synthase (MIPS, also known as IPS) isomerizes D-glucose-6-P to D-inositol 3-P, and this is the rate-limiting step in inositol production. In Arabidopsis thaliana, the MIPS enzyme is encoded by three different genes, (AtMIPS1, AtMIPS2 and AtMIPS3), each of which has been shown to produce proteins with biochemically similar properties but differential expression patterns...
May 18, 2018: Plant Molecular Biology
https://www.readbyqxmd.com/read/29777103/bacterial-encapsulins-as-orthogonal-compartments-for-mammalian-cell-engineering
#17
Felix Sigmund, Christoph Massner, Philipp Erdmann, Anja Stelzl, Hannes Rolbieski, Mitul Desai, Sarah Bricault, Tobias P Wörner, Joost Snijder, Arie Geerlof, Helmut Fuchs, Martin Hrabĕ de Angelis, Albert J R Heck, Alan Jasanoff, Vasilis Ntziachristos, Jürgen Plitzko, Gil G Westmeyer
We genetically controlled compartmentalization in eukaryotic cells by heterologous expression of bacterial encapsulin shell and cargo proteins to engineer enclosed enzymatic reactions and size-constrained metal biomineralization. The shell protein (EncA) from Myxococcus xanthus auto-assembles into nanocompartments inside mammalian cells to which sets of native (EncB,C,D) and engineered cargo proteins self-target enabling localized bimolecular fluorescence and enzyme complementation. Encapsulation of the enzyme tyrosinase leads to the confinement of toxic melanin production for robust detection via multispectral optoacoustic tomography (MSOT)...
May 18, 2018: Nature Communications
https://www.readbyqxmd.com/read/29776927/ecological-engineering-helps-maximize-function-in-algal-oil-production
#18
Sara L Jackrel, Anita Narwani, Bastian Bentlage, Robert B Levine, David C Hietala, Phillip E Savage, Todd H Oakley, Vincent J Denef, Bradley J Cardinale
Algal biofuels have the potential to curb emissions of greenhouse gases from fossil fuels, but current growing methods fail to produce fuels that meet the multiple standards necessary for economical industrial use. For example, algae grown as monocultures for biofuel production have not simultaneously and economically achieved high yields of the high-quality, lipid-rich biomass desired for the industrial-scale production of bio-oil. Decades of study in the field of ecology have demonstrated that simultaneous increases in multiple functions, such as the quantity and quality of biomass, can occur in natural ecosystems by increasing biological diversity...
May 18, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29776924/tools-for-rapid-genetic-engineering-of-vibrio-fischeri
#19
Karen L Visick, Kelsey M Hodge-Hanson, Alice H Tischler, Allison K Bennett, Vincent Mastrodomenico
Vibrio fischeri is used as a model for number of processes, including symbiosis, quorum sensing, bioluminescence, and biofilm formation. Many of these studies depend on generating deletion mutants and complementing them. Engineering such strains, however, is a time-consuming, multi-step process that relies on cloning and subcloning. Here, we describe a set of tools that can be used to rapidly engineer deletions and insertions in the V. fischeri chromosome without cloning. We developed a uniform approach for generating deletions using PCR SOEing (Splicing by Overlap Extension) with antibiotic cassettes flanked by standardized linker sequences...
May 18, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29775652/advances-and-prospects-of-bacillus-subtilis-cellular-factories-from-rational-design-to-industrial-applications
#20
REVIEW
Yang Gu, Xianhao Xu, Yaokang Wu, Tengfei Niu, Yanfeng Liu, Jianghua Li, Guocheng Du, Long Liu
Bacillus subtilis is the most characterized gram-positive bacterium that has significant attributes, such as growing well on cheap carbon sources, possessing clear inherited backgrounds, having mature genetic manipulation methods, and exhibiting robustness in large-scale fermentations. Till date, B. subtilis has been identified as attractive hosts for the production of recombinant proteins and chemicals. By applying various systems and synthetic biology tools, the productivity features of B. subtilis can be thoroughly analyzed and further optimized via metabolic engineering...
May 15, 2018: Metabolic Engineering
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