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https://www.readbyqxmd.com/read/28723213/dual-functional-core-shell-fluorescent-ag2s-carbon-nanostructure-for-selective-assay-of-e-coli-o157-h7-and-bactericidal-treatment
#1
Ning Wang, Xing Wei, An-Qi Zheng, Ting Yang, Ming-Li Chen, Jian-Hua Wang
A dual functional fluorescent core-shell Ag2S@Carbon nanostructure is prepared by a hydrothermally assisted multi-amino synthesis approach with folic acid (FA), polyethylenimine (PEI), and mannoses (Mans) as carbon and nitrogen sources (FA-PEI-Mans-Ag2S nanocomposite shortly as Ag2S@C). The nanostructure exhibits strong fluorescent emission at λex/λem = 340/450 nm with a quantum yield of 12.57 ± 0.52%. Ag2S@C is bound to E. coli O157:H7 via strong interaction with the Mans moiety in Ag2S@C with FimH proteins on the fimbriae tip in E...
March 24, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28683322/stromal-cell-derived-factor-1-mediates-immune-cell-attraction-upon-urinary-tract-infection
#2
Batya Isaacson, Tehila Hadad, Ariella Glasner, Chamutal Gur, Zvi Granot, Gilad Bachrach, Ofer Mandelboim
Urinary tract infection (UTI) is the most common type of bacterial infection in humans. Fifty percent of all women will experience at least one UTI in their lifetime, with uropathogenic Escherichia coli (UPEC) accounting for 80% of reported cases. UTI evokes a complex, well-timed immune response that is crucial for bacterial clearance. The majority of immune cells participating in the immune response are absent from the healthy bladder, and the mechanisms used to recruit them upon UTI are not fully understood...
July 5, 2017: Cell Reports
https://www.readbyqxmd.com/read/28681829/molecular-characterisation-of-uropathogenic-escherichia-coli-isolates-at-a-tertiary-care-hospital-in-south-india
#3
Arindam Chakraborty, Prabha Adhikari, Shalini Shenoy, Vishwas Saralaya
Uropathogenic Escherichia coli (UPEC) express a multitude of virulence factors (VFs) to break the inertia of the mucosal barrier of the urinary tract. The aim of the present study was undertaken to characterised the UPEC strains and to correlate carriage of specific virulence markers with different phylogroups and also to correlate these findings with clinical outcome of patients. A total of 156 non-repeated, clinically significant UPEC isolates were studied. Virulent genes were determined by two set of multiplex polymerase chain reaction (PCR)...
April 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28671638/sites-for-dynamic-protein-carbohydrate-interactions-of-o-and-c-linked-mannosides-on-the-e-coli-fimh-adhesin
#4
Mohamed Touaibia, Eva-Maria Krammer, Tze C Shiao, Nao Yamakawa, Qingan Wang, Anja Glinschert, Alex Papadopoulos, Leila Mousavifar, Emmanuel Maes, Stefan Oscarson, Gerard Vergoten, Marc F Lensink, René Roy, Julie Bouckaert
Antagonists of the Escherichia coli type-1 fimbrial adhesin FimH are recognized as attractive alternatives for antibiotic therapies and prophylaxes against acute and recurrent bacterial infections. In this study α-d-mannopyranosides O- or C-linked with an alkyl, alkene, alkyne, thioalkyl, amide, or sulfonamide were investigated to fit a hydrophobic substituent with up to two aryl groups within the tyrosine gate emerging from the mannose-binding pocket of FimH. The results were summarized into a set of structure-activity relationships to be used in FimH-targeted inhibitor design: alkene linkers gave an improved affinity and inhibitory potential, because of their relative flexibility combined with a favourable interaction with isoleucine-52 located in the middle of the tyrosine gate...
July 3, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28654733/target-directed-dynamic-combinatorial-chemistry-a-study-on-potentials-and-pitfalls-as-exemplified-on-a-bacterial-target
#5
Beat Ernst, Priska Frei, Lijuan Pang, Marleen Silbermann, Deniz Eris, Tobias Mühlethaler, Oliver Schwardt
Target-directed dynamic combinatorial chemistry (DCC) is an emerging technique for the efficient identification of inhibitors of pharmacologically relevant targets. In this contribution, we present an application for a bacterial target, the lectin FimH, a crucial virulence factor of uropathogenic E. coli being the main cause of urinary tract infections. A small dynamic library of acylhydrazones was formed from aldehydes and hydrazides and equilibrated at neutral pH in presence of aniline as nucleophilic catalyst...
June 27, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28614296/selective-depletion-of-uropathogenic-e-coli-from-the-gut-by-a-fimh-antagonist
#6
Caitlin N Spaulding, Roger D Klein, Ségolène Ruer, Andrew L Kau, Henry L Schreiber, Zachary T Cusumano, Karen W Dodson, Jerome S Pinkner, Daved H Fremont, James W Janetka, Han Remaut, Jeffrey I Gordon, Scott J Hultgren
Urinary tract infections (UTIs) caused by uropathogenic Escherichia coli (UPEC) affect 150 million people annually. Despite effective antibiotic therapy, 30-50% of patients experience recurrent UTIs. In addition, the growing prevalence of UPEC that are resistant to last-line antibiotic treatments, and more recently to carbapenems and colistin, make UTI a prime example of the antibiotic-resistance crisis and emphasize the need for new approaches to treat and prevent bacterial infections. UPEC strains establish reservoirs in the gut from which they are shed in the faeces, and can colonize the periurethral area or vagina and subsequently ascend through the urethra to the urinary tract, where they cause UTIs...
June 22, 2017: Nature
https://www.readbyqxmd.com/read/28596755/genetic-diversity-and-virulence-determinants-of-escherichia-coli-strains-isolated-from-patients-with-crohn-s-disease-in-spain-and-chile
#7
Sandra Céspedes, Waleska Saitz, Felipe Del Canto, Marjorie De la Fuente, Rodrigo Quera, Marcela Hermoso, Rául Muñoz, Daniel Ginard, Sam Khorrami, Jorge Girón, Rodrigo Assar, Ramón Rosselló-Mora, Roberto M Vidal
Adherent-invasive Escherichia coli (AIEC) strains are genetically variable and virulence factors for AIEC are non-specific. FimH is the most studied pathogenicity-related protein, and there have been few studies on other proteins, such as Serine Protease Autotransporters of Enterobacteriacea (SPATEs). The goal of this study is to characterize E. coli strains isolated from patients with Crohn's disease (CD) in Chile and Spain, and identify genetic differences between strains associated with virulence markers and clonality...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28592545/development-of-a-web-tool-for-escherichia-coli-sub-typing-based-on-fimh-alleles
#8
Louise Roer, Veronika Tchesnokova, Rosa Allesøe, Mariya Muradova, Sujay Chattopadhyay, Johanne Ahrenfeldt, Martin C F Thomsen, Ole Lund, Frank Hansen, Anette M Hammerum, Evgeni Sokurenko, Henrik Hasman
The aim of this study was to construct a valid publicly available method for in silico fimH sub-typing of Escherichia coli particularly suitable for differentiation of fine-resolution subgroups within clonal groups defined by standard multi-locus sequence typing (MLST). FimTyper was constructed as a FASTA database containing all currently known fimH alleles. The software source code is publicly available on https://bitbucket.org/genomicepidemiology/fimtyper, the database freely available at https://bitbucket...
June 7, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28584160/rapid-identification-of-different-escherichia-coli-st131-clades
#9
Yasufumi Matsumura, Johann D D Pitout, Gisele Peirano, Rebekah DeVinney, Taro Noguchi, Masaki Yamamoto, Ryota Gomi, Tomonari Matsuda, Satoshi Nakano, Miki Nagao, Michio Tanaka, Satoshi Ichiyama
Escherichia coli sequence type 131 (ST131) is a pandemic clonal lineage that is responsible for the global increase in fluoroquinolone-resistance and extended-spectrum β-lactamase (ESBL)-producers. The ST131 clade C, especially subclades C2 and C1-M27, are associated with ESBLs. We developed a multiplex conventional PCR assay with the ability to detect all ST131 clades (A, B, and C) as well as C subclades (C1-M27, C1-nM27 [C1-non-M27], and C2). To validate the assay, we used 80 ST131 global isolates that had been fully sequenced...
June 5, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28584139/genomic-analysis-of-multidrug-resistant-escherichia-coli-from-north-carolina-community-hospitals-ongoing-circulation-of-ctx-m-producing-st131-h30rx-and-st131-h30r1-strains
#10
Hajime Kanamori, Christian M Parobek, Jonathan J Juliano, James R Johnson, Brian D Johnston, Timothy J Johnson, David J Weber, William A Rutala, Deverick J Anderson
Escherichia coli sequence type 131 (ST131) predominates globally among multidrug-resistant (MDR) E. coli We used whole-genome sequencing (WGS) to investigate 63 MDR E. coli isolates from 7 North Carolina community hospitals (2010-2015). Of these, 39 (62%) represented ST131, including 37 (95%) from the ST131-H30R subclone: 10 (27%) from its H30R1 subset, and 27 (69%) from its H30Rx subset. ST131 core genomes differed by a median of 15 single-nucleotide variants (SNVs) overall (range, 0-490), vs. only 7 within H30R1 (range, 3-12 SNVs) and 11 within H30Rx (range, 0-21)...
June 5, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28560183/outbreak-by-hypermucoviscous-klebsiella-pneumoniae-st11-isolates-with-carbapenem-resistance-in-a-tertiary-hospital-in-china
#11
Lingling Zhan, Shanshan Wang, Yinjuan Guo, Ye Jin, Jingjing Duan, Zhihao Hao, Jingnan Lv, Xiuqin Qi, Longhua Hu, Liang Chen, Barry N Kreiswirth, Rong Zhang, Jingye Pan, Liangxing Wang, Fangyou Yu
Hypervirulent and multidrug resistant Klebsiella pneumoniae strains pose a significant threat to the public health. In the present study, 21 carbapenem-resistant K. pneumoniae isolates (CRKP) were determined by the string test as hypermucoviscous K. pneumoniae (HMKP), with the prevalence of 15.0% (21/140) among CRKP, and 1.1% (21/1838) among all K. pneumoniae isolates. Among them, 7 (33.3%), and 1 (4.76%) isolate belonged to capsular serotype K20 and K2 respectively, while 13 (61.9%, 13/21) weren't successfully typed by capsular serotyping...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28552831/extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-isolated-from-healthy-humans-in-mexico-including-subclone-st131-b2-o25-h4-h30-rx
#12
Gerardo Cortés-Cortés, Patricia Lozano-Zarain, Carmen Torres, Carla Andrea Alonso, Ana María Ríos-Torres, Miguel Castañeda, Liliana López-Pliego, Armando Navarro, Rosa Del Carmen Rocha-Gracia
OBJECTIVES: The resistance mechanisms, molecular type and plasmid content of cefotaxime-resistant Escherichia coli isolated from faecal samples of healthy volunteers in Puebla, Mexico, were characterised. METHODS AND RESULTS: Cefotaxime-resistant E. coli were recovered from 11 (18%) of 60 healthy volunteers. The isolates (one per sample) were characterised as multidrug-resistant and phenotypically extended-spectrum β-lactamase (ESBL)-producing strains. Genes encoding resistance to β-lactams (blaCTX-M-15, blaCTX-M-14a, blaCTX-M-14b, blaOXA-1, blaTEM-1b), quinolones [aac(6')-Ib-cr, qnrB19], aminoglycosides [aac(3')-II] and tetracycline [tet(A), tet(B)] were detected among the 11 ESBL-producing E...
June 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28545489/cytotoxic-necrotizing-factor-1-cnf1-does-not-promote-e-coli-infection-in-a-murine-model-of-ascending-pyelonephritis
#13
Jason E Michaud, Kwang Sik Kim, William Harty, Matthew Kasprenski, Ming-Hsien Wang
BACKGROUND: Urinary tract infections (UTI) are among the most common and costly infections in both hospitalized and ambulatory patients. Uropathogenic E. coli (UPEC) represent the majority of UTI isolates and are a diverse group of bacteria that utilize a variety of virulence factors to establish infection of the genitourinary tract. The virulence factor cytotoxic necrotizing factor-1 (CNF1) is frequently expressed in clinical UPEC isolates. To date, there have been conflicting reports on the role of CNF1 in the pathogenesis of E...
May 25, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28531220/highly-conserved-type-1-pili-promote-enterotoxigenic-e-coli-pathogen-host-interactions
#14
Alaullah Sheikh, Rasheduzzaman Rashu, Yasmin Ara Begum, F Matthew Kuhlman, Matthew A Ciorba, Scott J Hultgren, Firdausi Qadri, James M Fleckenstein
Enterotoxigenic Escherichia coli (ETEC), defined by their elaboration of heat-labile (LT) and/or heat-stable (ST) enterotoxins, are a common cause of diarrheal illness in developing countries. Efficient delivery of these toxins requires ETEC to engage target host enterocytes. This engagement is accomplished using a variety of pathovar-specific and conserved E. coli adhesin molecules as well as plasmid encoded colonization factors. Some of these adhesins undergo significant transcriptional modulation as ETEC encounter intestinal epithelia, perhaps suggesting that they cooperatively facilitate interaction with the host...
May 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28527285/-genetic-diversity-of-extraintestinal-escherichia-coli-strains-producers-of-beta-lactamases-tem-shv-and-ctx-m-associated-with-healthcare
#15
Yasmin Varela, Beatriz Millán, María Araque
INTRODUCTION: There are few reports from Venezuela describing the genetic basis that sustains the pathogenic potential and phylogenetics of Escherichia coli extraintestinal strains isolated in health care units. OBJECTIVE: To establish the genetic diversity of extraintestinal E. coli strains producers of betalactamases TEM, SHV and CTX-M associated with healthcare. MATERIALS AND METHODS: We studied a collection of 12 strains of extraintestinal E...
June 1, 2017: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/28506090/rational-design-strategies-for-fimh-antagonists-new-drugs-on-the-horizon-for-urinary-tract-infection-and-crohn-s-disease
#16
REVIEW
Laurel K Mydock-McGrane, Thomas J Hannan, James W Janetka
The bacterial adhesin FimH is a virulence factor and an attractive therapeutic target for urinary tract infection (UTI) and Crohn's Disease (CD). Located on type 1 pili of uropathogenic E. coli (UPEC), the FimH adhesin plays an integral role in the pathogenesis of UPEC. Recent efforts have culminated in the development of small-molecule mannoside FimH antagonists that target the mannose-binding lectin domain of FimH, inhibiting its function and preventing UPEC from binding mannosylated host cells in the bladder, thereby circumventing infection...
July 2017: Expert Opinion on Drug Discovery
https://www.readbyqxmd.com/read/28490179/-living-dynamics-of-filamentous-bacteria-on-an-adherent-surface-under-hydrodynamic-exposure
#17
Justin P Jahnke, Austin M Smith, Nicole E Zander, Victoria Wiedorn, Kenneth E Strawhecker, Jessica L Terrell, Dimitra N Stratis-Cullum, Xuanhong Cheng
The potential advantages of cell-based biohybrid devices over conventional nonliving systems drive the interest to control the behavior of the underlying biological cells in microdevices. Here, the authors studied how shear influenced the geometry and elongation of fimbriated filaments on affinity substrates. The cells were engineered to express FimH, which binds to mannose with a high affinity. A microfluidic channel was functionalized with RNAse B, which is rich in mannose residues, and the device was used to control the hydrodynamic force on live Escherichia coli under filamentous growth...
May 10, 2017: Biointerphases
https://www.readbyqxmd.com/read/28478807/aqueous-extract-from-orthosiphon-stamineus-leaves-prevents-bladder-and-kidney-infection-in-mice
#18
S Sarshar, S Brandt, M R Asadi Karam, M Habibi, S Bouzari, M Lechtenberg, U Dobrindt, X Qin, F M Goycoolea, A Hensel
BACKGROUND: Extracts from the leaves of Orthosiphon stamineus are used in phytotherapy for treatment of uncomplicated urinary tract infections. PURPOSES: Evaluation of an aqueous extract against infection with uropathogenic Escherichia coli in vivo; investigation of underlying microbiological mechanisms. STUDY DESIGN: In vivo studies in mice and in vitro investigations on cytotoxicity, antiadhesive potential, influence on bacterial gene expression and quorum sensing...
May 15, 2017: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/28471659/urinary-tract-infection-which-conformation-of-the-bacterial-lectin-fimh-is-therapeutically-relevant
#19
Katharina Mayer, Deniz Eris, Oliver Schwardt, Christoph P Sager, Said Rabbani, Simon Kleeb, Beat Ernst
Frequent antibiotic treatment of urinary tract infections has resulted in the emergence of antimicrobial resistance, necessitating alternative treatment options. One such approach centers around FimH antagonists that block the bacterial adhesin FimH, which would otherwise mediate binding of uropathogenic Escherichia coli to the host urothelium to trigger the infection. Although the FimH lectin can adopt three distinct conformations, the evaluation of FimH antagonists has mainly been performed with a truncated construct of FimH locked in one particular conformation...
May 11, 2017: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28459221/association-between-virulence-profile-and-fluoroquinolone-resistance-in-escherichia-coli-isolated-from-dogs-and-cats-in-china
#20
Xiaoqiang Liu, Haixia Liu, Yinqian Li, Caiju Hao
INTRODUCTION: Escherichia coli is not only a commensal organism in humans and animals, but also a causative agent of diarrhea and extraintestinal infections. Information about the relationship between population structure, virulence gene profiles, and fluoroquinolone resistance of E. coli in dogs and cats in China is limited. METHODOLOGY: A total of 174 pathogenic and commensal E. coli isolates were evaluated in terms of phylogenetic group, virulence gene profile, sequence types (STs), and fluoroquinolone susceptibility...
April 30, 2017: Journal of Infection in Developing Countries
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