keyword
https://read.qxmd.com/read/38532498/the-spontaneously-produced-lysogenic-prophage-phi456-promotes-bacterial-resistance-to-adverse-environments-and-enhances-the-colonization-ability-of-avian-pathogenic-escherichia-coli-strain-de456
#1
JOURNAL ARTICLE
Dezhi Li, Wei Liang, Zhiqiang Huang, Wenwen Ma, Qing Liu
In the last decade, prophages that possess the ability of lysogenic transformation have become increasingly significant. Their transfer and subsequent activity in the host have a significant impact on the evolution of bacteria. Here, we investigate the role of prophage phi456 with high spontaneous induction in the bacterial genome of Avian pathogenic Escherichia coli (APEC) DE456. The phage particles, phi456, that were released from DE456 were isolated, purified, and sequenced. Additionally, phage particles were no longer observed either during normal growth or induced by nalidixic acid in DE456Δphi456...
March 26, 2024: Veterinary Research
https://read.qxmd.com/read/38520165/virulent-klebsiella-pneumoniae-st11-clone-carrying-blakpc-and-blandm-from-patients-with-and-without-covid-19-in-brazil
#2
JOURNAL ARTICLE
Maria Izabely Silva Pimentel, Elizabeth Maria Bispo Beltrão, Érica Maria de Oliveira, Lamartine Rodrigues Martins, Moacir Batista Jucá, Ana Catarina de Souza Lopes
AIMS: Investigated and compared the occurrence of virulence genes fimH, mrkD, irp2, entB, cps, rmpA and wabG, resistance genes blaKPC and blaNDM, and the genetic variability and clonal relationship of 29 Klebsiella pneumoniae clinical isolates of patients with and without COVID-19, from a hospital in Brazil. METHODS AND RESULTS: All isolates were resistant to beta-lactams. The genes were investigated by PCR, and for molecular typing, ERIC-PCR and MLST were used...
March 22, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38483200/bioinspired-nanozymes-as-nanodecoys-for-urinary-tract-infection-treatment
#3
JOURNAL ARTICLE
Yihong Zhang, Wanling Liu, Gen Wei, Quanyi Liu, Guoqiang Shao, Xiang Gu, Xiaomiao Cui, Zijun Zhou, Yuting Wang, Sheng Zhao, Faheem Muhammad, Sirong Li, Tong Li, Yan Du, Hui Wei
Urinary tract infections (UTIs), common bacterial infections in communities and medical facilities, are mainly mediated by FimH. The glycan sites of the uromodulin protein play a crucial role in protecting against UTIs by interacting with FimH. A bioinspired approach using glycan-FimH interactions may effectively reduce bacteria through an antiadhesive mechanism, thereby curbing bacterial resistance. However, typical antiadhesive therapy alone fails to address the excessive reactive oxygen species and inflammatory response during UTIs...
March 14, 2024: ACS Nano
https://read.qxmd.com/read/38479049/comprehensive-analysis-of-multiple-cytokines-to-stratify-uropathogenic-escherichia-coli-pathogenesis-in-mouse-model-of-urinary-tract-infection
#4
JOURNAL ARTICLE
Snehashis Koley, Mandira Mukherjee
PURPOSE: Urinary tract infection (UTI) is one of the most common human bacterial infections primarily caused by uropathogenic E. coli (UPEC). Empiric treatment in UTI cause emergence of multidrug resistance and limit treatment options. Understanding UTI at the molecular level with respect to the causative pathogen as well as subsequent host response pose an absolute necessity towards appropriate clinical management. This study aimed to investigate host cytokine response in mouse UTI model with respect to bacterial colonization and associated virulence gene expression upon infection...
March 12, 2024: Cytokine
https://read.qxmd.com/read/38454747/sweet-janus-particles-multifunctional-inhibitors-of-carbohydrate-based-bacterial-adhesion
#5
JOURNAL ARTICLE
Serap Üclü, Claudia Marschelke, Felictas Drees, Markus Giesler, Dimitri Wilms, Thorben Köhler, Stephan Schmidt, Alla Synytska, Laura Hartmann
Escherichia coli and other bacteria use adhesion receptors, such as FimH, to attach to carbohydrates on the cell surface as the first step of colonization and infection. Efficient inhibitors that block these interactions for infection treatment are multivalent carbohydrate-functionalized scaffolds. However, these multivalent systems often lead to the formation of large clusters of bacteria, which may pose problems for clearing bacteria from the infected site. Here, we present Man-containing Janus particles (JPs) decorated on one side with glycomacromolecules to target Man-specific adhesion receptors of E...
March 7, 2024: Biomacromolecules
https://read.qxmd.com/read/38447463/design-synthesis-biological-evaluation-and-docking-study-of-some-new-aryl-and-heteroaryl-thiomannosides-as-fimh-antagonists
#6
JOURNAL ARTICLE
Anber F Mohammed, Shimaa A Othman, Ola F Abou-Ghadir, Ahmed A Kotb, Yaser A Mostafa, Mohamed A El-Mokhtar, Hajjaj H M Abdu-Allah
FimH is a mannose-recognizing lectin that is expressed by Escherichia coli guiding its ability to adhere and infect cells. It is involved in pathogenesis of urinary tract infections and Chron's disease. Several X-ray structure-guided ligand design studies were extensively utilized in the discovery and optimization of small molecule aryl mannoside FimH antagonists. These antagonists retain key specific interactions of the mannose scaffolds with the FimH carbohydrate recognition domains. Thiomannosides are attractive and stable scaffolds, and this work reports the synthesis of some of their new aryl and heteroaryl derivatives as FimH antagonists...
March 2, 2024: Bioorganic Chemistry
https://read.qxmd.com/read/38442898/selective-quantification-of-bacteria-in-mixtures-by-using-glycosylated-polypyrrole-hydrogel-nanolayers
#7
JOURNAL ARTICLE
Sebastian Balser, Michael Röhrl, Carina Spormann, Thisbe K Lindhorst, Andreas Terfort
Here, we present a covalent nanolayer system that consists of a conductive and biorepulsive base layer topped by a layer carrying biorecognition sites. The layers are built up by electropolymerization of pyrrole derivatives that either carry polyglycerol brushes (for biorepulsivity) or glycoside moieties (as biorecognition sites). The polypyrrole backbone makes the resulting nanolayer systems conductive, opening the opportunity for constructing an electrochemistry-based sensor system. The basic concept of the sensor exploits the highly selective binding of carbohydrates by certain harmful bacteria, as bacterial adhesion and infection are a major threat to human health, and thus, a sensitive and selective detection of the respective bacteria by portable devices is highly desirable...
March 5, 2024: ACS Applied Materials & Interfaces
https://read.qxmd.com/read/38438447/potent-biological-activity-of-newly-fabricated-silver-nanoparticles-coated-by-a-carbon-shell-synthesized-by-electrical-arc
#8
JOURNAL ARTICLE
Bassma H Elwakil, Ahmed M Eldrieny, Awatif Rashed Z Almotairy, Mostafa El-Khatib
Highly effective AgNPs@C was efficiently synthesized by electrical arc powered by single spark unit which was sufficient to ionize the dielectric media (deionized water) through applying strong electric field between the electrodes (silver and carbon). The AgNPs@C shell was characterized in terms of stability, morphology and phase structure. All characterizations showed that the prepared silver nanoparticles were spherical with average size reached 17 nm coated with carbon shell. The antibacterial effect of the synthesized nanoparticles was tested against Pseudomonas aeruginosa in comparison to Ceftazidime (commonly used antibiotic against P...
March 4, 2024: Scientific Reports
https://read.qxmd.com/read/38367495/does-size-matter-comparing-pyranoses-with-septanoses-as-ligands-of-the-bacterial-lectin-fimh
#9
JOURNAL ARTICLE
Jonathan Cramer, Bryant Pero, Xiaohua Jiang, Cristin Bosko, Marleen Silbermann, Said Rabbani, Sebastian Wilke, Dilara D Nemli, Beat Ernst, Mark W Peczuh
The pharmacological modulation of disease-relevant carbohydrate-protein interactions represents an underexplored area of medicinal chemistry. One particular challenge in the design of glycomimetic compounds is the inherent instability of the glycosidic bond toward enzymatic cleavage. This problem has traditionally been approached by employing S-, N-, or C-glycosides with reduced susceptibility toward glycosidases. The application of ring-extended glycomimetics is an innovative approach to circumvent this issue...
February 8, 2024: European Journal of Medicinal Chemistry
https://read.qxmd.com/read/38357165/relationship-between-antimicrobial-resistance-and-virulence-factors-in-uropathogenic-escherichia-coli-isolates-from-ramadi-iraq-phenotype-and-genotype-identification
#10
JOURNAL ARTICLE
Ahmed Dhahir Abed, Thamer Y Mutter
BACKGROUND: Uropathogenic Escherichia coli (UPEC) is the most predominant pathogen that causes severe urinary tract infections (UTIs). Their therapeutic options are limited due to the rising of antibiotic resistance. OBJECTIVE: The aim of the study was to evaluate the level of antibiotic resistance profile, redundancy of virulence genes, and their correlation. METHODS: 41 UPEC isolates were collected from patients diagnosed with UTI, identified by the standard microbiological analysis, and tested for susceptibility to 12 antibiotic agents using the Kirby-Bauer method...
September 2023: African Health Sciences
https://read.qxmd.com/read/38345976/the-correlation-between-adhesion-genes-and-biofilm-formation-among-escherichia-coli-clinical-isolates
#11
JOURNAL ARTICLE
Hadeel K Musafer, Firas N Jaafar, Marwa H M Alkhafaji
BACKGROUND: The adhesion genes are responsible for biofilm production which leads to chronic diseases like urinary tract infections (UTIs). Uropathogenic Escherichia coli (UPEC) is the most predominant pathogen involved in UTIs. This study aims to evaluate the relationship between adhesion genes and bacterial biofilm that form by UPEC. METHODS: Fifty clinical isolates of E. coli from patients infected with UTIs were identified and antimicrobial resistance was tested by MIC assay...
February 1, 2024: Clinical Laboratory
https://read.qxmd.com/read/38293315/higher-virulence-renders-k2-klebsiella-pneumoniae-a-stable-share-among-those-from-pyogenic-liver-abscess
#12
JOURNAL ARTICLE
Min Dong, Xiumin Ma, Donglian Wang, Xiaobo Ma, Jin Zhang, Lianhua Yu, Qing Yang, Dakang Hu, Dengyan Qiao
OBJECTIVE: To explore why serotype K2 accounts for a stable share in Klebsiella pneumoniae from pyogenic liver abscess (PLA). METHODS: Totally 15 K2 K. pneumoniae strains from PLA, 21 K2 from non-PLA, and 31 K1 from PLA were collected from China. Sequence typing, molecular serotyping, regular PCR, and Galleria mellonella lethality were performed. A total of 12 virulence genes were detected: peg-344, allS, p-rmpA, p-rmpA2, c-rmpA, fimH, mrkD, iucA, iroN, irp2, entB , and wzi ...
2024: Infection and Drug Resistance
https://read.qxmd.com/read/38227590/occurrence-and-characteristics-of-extended-spectrum-%C3%AE-lactamase-and-pampc-producing-klebsiella-pneumoniae-isolated-from-companion-animals-with-urinary-tract-infections
#13
JOURNAL ARTICLE
Megan Min Yi Lee, Nan-Ling Kuan, Zhi-Yi Li, Kuang-Sheng Yeh
This study examined 70 Klebsiella pneumoniae isolates derived from companion animals with urinary tract infections in Taiwan. Overall, 81% (57/70) of the isolates carried extended-spectrum β-lactamase (ESBL) and/or plasmid-encoded AmpC (pAmpC) genes. ESBL genes were detected in 19 samples, with blaCTX-M-1, blaCTX-M-9, and blaSHV being the predominant groups. pAmpC genes were detected in 56 isolates, with blaCIT and blaDHA being the predominant groups. Multilocus sequence typing revealed that sequence types (ST)11, ST15, and ST655 were prevalent...
2024: PloS One
https://read.qxmd.com/read/38193668/-escherichia-coli-sequence-type-410-with-carbapenemases-a-paradigm-shift-within-e-coli-toward-multidrug-resistance
#14
REVIEW
Johann D D Pitout, Gisele Peirano, Yasufumi Matsumura, Rebekah DeVinney, Liang Chen
Escherichia coli sequence type ST410 is an emerging carbapenemase-producing multidrug-resistant (MDR) high-risk One-Health clone with the potential to significantly increase carbapenem resistance among E. coli . ST410 belongs to two clades (ST410-A and ST410-B) and three subclades (ST410-B1, ST410-B2, and ST410-B3). After a fimH switch between clades ST410-A and ST410-B1, ST410-B2 and ST410-B3 subclades showed a stepwise progression toward developing MDR. (i) ST410-B2 initially acquired fluoroquinolone resistance (via homologous recombination) in the 1980s...
January 9, 2024: Antimicrobial Agents and Chemotherapy
https://read.qxmd.com/read/38140179/-lactobacillus-acidophilus-expressing-murine-rotavirus-vp8-and-mucosal-adjuvants-induce-virus-specific-immune-responses
#15
JOURNAL ARTICLE
Darby Gilfillan, Allison C Vilander, Meichen Pan, Yong Jun Goh, Sarah O'Flaherty, Ningguo Feng, Bridget E Fox, Callie Lang, Harry B Greenberg, Zaid Abdo, Rodolphe Barrangou, Gregg A Dean
Rotavirus diarrhea-associated illness remains a major cause of global death in children under five, attributable in part to discrepancies in vaccine performance between high- and low-middle-income countries. Next-generation probiotic vaccines could help bridge this efficacy gap. We developed a novel recombinant Lactobacillus acidophilus (rLA) vaccine expressing rotavirus antigens of the VP8* domain from the rotavirus EDIM VP4 capsid protein along with the adjuvants FimH and FliC. The upp -based counterselective gene-replacement system was used to chromosomally integrate FimH, VP8Pep (10 amino acid epitope), and VP8-1 (206 amino acid protein) into the L...
November 28, 2023: Vaccines
https://read.qxmd.com/read/38138002/biofilm-formation-by-escherichia-coli-isolated-from-urinary-tract-infections-from-aguascalientes-mexico
#16
JOURNAL ARTICLE
Flor Yazmín Ramírez Castillo, Alma Lilian Guerrero Barrera, Josée Harel, Francisco Javier Avelar González, Philippe Vogeleer, José Manuel Arreola Guerra, Mario González Gámez
Uropathogenic Escherichia coli (UPEC) strains are among the leading causes of urinary tract infections (UTIs) worldwide. They can colonize the urinary tract and form biofilms that allow bacteria to survive and persist, causing relapses of infections and life-threatening sequelae. Here, we analyzed biofilm production, antimicrobial susceptibility, virulence factors, and phylogenetic groups in 74 E. coli isolated from diagnosed patients with UTIs to describe their microbiological features and ascertain their relationship with biofilm capabilities...
November 25, 2023: Microorganisms
https://read.qxmd.com/read/38117954/investigating-the-effects-of-zinc-oxide-and-titanium-dioxide-nanoparticles-on-the-formation-of-biofilm-and-persister-cells-in-klebsiella-pneumoniae
#17
JOURNAL ARTICLE
Aisouda Pourmehdiabadi, Mojgan Sarabi Nobakht, Behdad Hajjam Balajorshari, Mohammadreza Rezaei Yazdi, Kumarss Amini
The biofilm formation in klebsiella pneumoniae isolates poses a significant problem as it can result in treatment failure and the development of chronic infections. These biofilms act as protective barriers, rendering the bacteria resistant to antibiotics. Additionally, persister cells, which make up a small fraction of the bacterial population, have the ability to enter a dormant state after treatment with high doses of antibiotics. These persister cells play a crucial role in the high level of biofilm-mediated tolerance to antibiotics...
December 20, 2023: Journal of Basic Microbiology
https://read.qxmd.com/read/38117675/genomic-characterization-of-bordetella-pertussis-in-south-africa-2015-2019
#18
JOURNAL ARTICLE
Fahima Moosa, Mignon du Plessis, Michael R Weigand, Yanhui Peng, Dineo Mogale, Linda de Gouveia, Marta C Nunes, Shabir A Madhi, Heather J Zar, Gary Reubenson, Arshad Ismail, M Lucia Tondella, Cheryl Cohen, Sibongile Walaza, Anne von Gottberg, Nicole Wolter
Pertussis remains a public health concern in South Africa, with an increase in reported cases and outbreaks in recent years. Whole genome sequencing was performed on 32 Bordetella pertussis isolates sourced from three different surveillance programmes in South Africa between 2015 and 2019. Genome sequences were characterized using multilocus sequence typing, vaccine antigen genes ( ptxP , ptxA , ptxB , prn and fimH ) and overall genome structure. All isolates were sequence type 2 and harboured the pertussis toxin promoter allele ptxP3 ...
December 2023: Microbial Genomics
https://read.qxmd.com/read/38091178/the-secrets-of-environmental-pseudomonas-aeruginosa-in-slaughterhouses-antibiogram-profile-virulence-and-antibiotic-resistance-genes
#19
JOURNAL ARTICLE
Israa M S Al-Kadmy, Suhad Abbas Abid, Sarah Naji Aziz, Zahraa Al-Kadmy, Ahmed Suhail, Sawsan Sajid Al-Jubori, Eman Natiq Naji, Eman Alhomaidi, Ramadan Yahia, Abdelazeem M Algammal, Gaber El-Saber Batiha, Helal F Hetta
Environmental pollution is a serious problem that can cause sicknesses, fatality, and biological contaminants such as bacteria, which can trigger allergic reactions and infectious illnesses. There is also evidence that environmental pollutants can have an impact on the gut microbiome and contribute to the development of various mental health and metabolic disorders. This study aimed to study the antibiotic resistance and virulence potential of environmental Pseudomonas aeruginosa (P. aeruginosa) isolates in slaughterhouses...
December 13, 2023: Folia Microbiologica
https://read.qxmd.com/read/38077339/-escherichia-coli-adhesion-protein-fimh-exacerbates-colitis-via-cd11b-cd103-dendritic-cell-activation
#20
JOURNAL ARTICLE
Wei Zhang, Eun-Koung An, So-Jung Kim, Hae-Bin Park, Peter C W Lee, Jun-O Jin
INTRODUCTION: Immune stimulators are used to improve vaccine efficiency; however, they are accompanied by various side effects. In previous studies, we reported that the Escherichia coli adhesion protein, FimH, induces immune activity; however, we did not examine any side effects in colon inflammation. METHODS: FimH was administered orally or intraperitoneally ( i.p. ) to mice with dextran sulfate sodium (DSS)-induced colitis, and changes in symptoms were observed...
2023: Frontiers in Immunology
keyword
keyword
18003
1
2
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.