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https://www.readbyqxmd.com/read/28808163/a-role-for-fimh-in-extraintestinal-pathogenic-escherichia-coli-invasion-and-translocation-through-the-intestinal-epithelium
#1
Nina M Poole, Sabrina I Green, Anubama Rajan, Luz E Vela, Xi-Lei Zeng, Mary K Estes, Anthony W Maresso
The translocation of bacteria across the intestinal epithelium of immunocompromised patients can lead to bacteremia and life-threatening sepsis. Extraintestinal Pathogenic Escherichia coli (ExPEC), so named because this pathotype infects tissues distal to the intestinal tract, is a frequent cause of such infections, is often multidrug resistant, and chronically colonizes a sizable portion of the healthy population. Although several virulence factors and their roles in pathogenesis are well described for ExPEC that cause urinary tract infections and meningitis, they have not been linked to translocation through intestinal barriers, a fundamentally distant yet important clinical phenomenon...
August 14, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28770174/the-novel-type-1-fimbriae-fimh-receptor-calreticulin-plays-a-role-in-salmonella-host-specificity
#2
Krzysztof Grzymajlo, Maciej Ugorski, Jaroslaw Suchanski, Anna E Kedzierska, Rafal Kolenda, Anna Jarzab, Agnieszka Biernatowska, Peter Schierack
It was suggested that minor differences in the structure of FimH are most likely associated with differences in its adhesion specificities and may determine the tropism of various Salmonella serovars to different species and tissues. We have recently shown that FimH adhesins from host-adapted serovars, e.g., Salmonella Choleraesuis (SCh), bind to other glycoprotein receptors compared to FimH from host-unrestricted Salmonella Enteritidis (SE). Here we identify porcine calreticulin expressed by swine intestinal cells as a host-specific receptor for SCh FimH adhesin, suggesting that such an interaction may contribute to SCh host specificity...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28762293/the-potential-of-fimh-as-a-novel-therapeutic-target-for-the-treatment-of-crohn-s-disease
#3
Adeline Sivignon, Julie Bouckaert, Julien Bernard, Sebastien G Gouin, Nicolas Barnich
Crohn's disease (CD) is a life-long chronic disorder characterized by intestinal inflammation. Current treatments for CD are directed towards abnormal immune responses rather than the intestinal bacteria that trigger intestinal inflammation. Areas covered: Adherent-Invasive Escherichia coli (AIEC) bacteria abnormally colonize the ileal mucosa in a subgroup of CD patients. They can promote or perpetuate chronic inflammation and are therefore an interesting therapeutic target. Various strategies that target these E...
August 1, 2017: Expert Opinion on Therapeutic Targets
https://www.readbyqxmd.com/read/28760455/association-between-virulence-profile-biofilm-formation-and-phylogenetic-groups-of-escherichia-coli-causing-urinary-tract-infection-and-the-commensal-gut-microbiota-a-comparative-analysis
#4
Zahra Hashemizadeh, Davood Kalantar-Neyestanaki, Shahla Mansouri
Variety of virulence factors are involved in the pathogenicity of Escherichia coli, the common cause of the urinary tract infections (UTIs). The aim of this study was to determine some virulence factors involved in the pathogenicity and the phylogenetic grouping of E. coli from UTIs compared with the E. coli isolates from gut microbiota (fecal flora). The isolates were tested for biofilm formation, haemagglutination, cell surface hydrophobicity (CSH), hemolysin production, phylogenetic grouping and the distribution of 6 known virulence genes...
July 29, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28723213/dual-functional-core-shell-fluorescent-ag2s-carbon-nanostructure-for-selective-assay-of-e-coli-o157-h7-and-bactericidal-treatment
#5
Ning Wang, Xing Wei, An-Qi Zheng, Ting Yang, Ming-Li Chen, Jian-Hua Wang
A dual functional fluorescent core-shell Ag2S@Carbon nanostructure is prepared by a hydrothermally assisted multi-amino synthesis approach with folic acid (FA), polyethylenimine (PEI), and mannoses (Mans) as carbon and nitrogen sources (FA-PEI-Mans-Ag2S nanocomposite shortly as Ag2S@C). The nanostructure exhibits strong fluorescent emission at λex/λem = 340/450 nm with a quantum yield of 12.57 ± 0.52%. Ag2S@C is bound to E. coli O157:H7 via strong interaction with the Mans moiety in Ag2S@C with FimH proteins on the fimbriae tip in E...
March 24, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28683322/stromal-cell-derived-factor-1-mediates-immune-cell-attraction-upon-urinary-tract-infection
#6
Batya Isaacson, Tehila Hadad, Ariella Glasner, Chamutal Gur, Zvi Granot, Gilad Bachrach, Ofer Mandelboim
Urinary tract infection (UTI) is the most common type of bacterial infection in humans. Fifty percent of all women will experience at least one UTI in their lifetime, with uropathogenic Escherichia coli (UPEC) accounting for 80% of reported cases. UTI evokes a complex, well-timed immune response that is crucial for bacterial clearance. The majority of immune cells participating in the immune response are absent from the healthy bladder, and the mechanisms used to recruit them upon UTI are not fully understood...
July 5, 2017: Cell Reports
https://www.readbyqxmd.com/read/28681829/molecular-characterisation-of-uropathogenic-escherichia-coli-isolates-at-a-tertiary-care-hospital-in-south-india
#7
Arindam Chakraborty, Prabha Adhikari, Shalini Shenoy, Vishwas Saralaya
Uropathogenic Escherichia coli (UPEC) express a multitude of virulence factors (VFs) to break the inertia of the mucosal barrier of the urinary tract. The aim of the present study was undertaken to characterised the UPEC strains and to correlate carriage of specific virulence markers with different phylogroups and also to correlate these findings with clinical outcome of patients. A total of 156 non-repeated, clinically significant UPEC isolates were studied. Virulent genes were determined by two set of multiplex polymerase chain reaction (PCR)...
April 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28671638/sites-for-dynamic-protein-carbohydrate-interactions-of-o-and-c-linked-mannosides-on-the-e-coli-fimh-adhesin
#8
Mohamed Touaibia, Eva-Maria Krammer, Tze C Shiao, Nao Yamakawa, Qingan Wang, Anja Glinschert, Alex Papadopoulos, Leila Mousavifar, Emmanuel Maes, Stefan Oscarson, Gerard Vergoten, Marc F Lensink, René Roy, Julie Bouckaert
Antagonists of the Escherichia coli type-1 fimbrial adhesin FimH are recognized as attractive alternatives for antibiotic therapies and prophylaxes against acute and recurrent bacterial infections. In this study α-d-mannopyranosides O- or C-linked with an alkyl, alkene, alkyne, thioalkyl, amide, or sulfonamide were investigated to fit a hydrophobic substituent with up to two aryl groups within the tyrosine gate emerging from the mannose-binding pocket of FimH. The results were summarized into a set of structure-activity relationships to be used in FimH-targeted inhibitor design: alkene linkers gave an improved affinity and inhibitory potential, because of their relative flexibility combined with a favourable interaction with isoleucine-52 located in the middle of the tyrosine gate...
July 3, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28654733/target-directed-dynamic-combinatorial-chemistry-a-study-on-potentials-and-pitfalls-as-exemplified-on-a-bacterial-target
#9
Beat Ernst, Priska Frei, Lijuan Pang, Marleen Silbermann, Deniz Eris, Tobias Mühlethaler, Oliver Schwardt
Target-directed dynamic combinatorial chemistry (DCC) is an emerging technique for the efficient identification of inhibitors of pharmacologically relevant targets. In this contribution, we present an application for a bacterial target, the lectin FimH, a crucial virulence factor of uropathogenic E. coli being the main cause of urinary tract infections. A small dynamic library of acylhydrazones was formed from aldehydes and hydrazides and equilibrated at neutral pH in presence of aniline as nucleophilic catalyst...
June 27, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28614296/selective-depletion-of-uropathogenic-e-coli-from-the-gut-by-a-fimh-antagonist
#10
Caitlin N Spaulding, Roger D Klein, Ségolène Ruer, Andrew L Kau, Henry L Schreiber, Zachary T Cusumano, Karen W Dodson, Jerome S Pinkner, Daved H Fremont, James W Janetka, Han Remaut, Jeffrey I Gordon, Scott J Hultgren
Urinary tract infections (UTIs) caused by uropathogenic Escherichia coli (UPEC) affect 150 million people annually. Despite effective antibiotic therapy, 30-50% of patients experience recurrent UTIs. In addition, the growing prevalence of UPEC that are resistant to last-line antibiotic treatments, and more recently to carbapenems and colistin, make UTI a prime example of the antibiotic-resistance crisis and emphasize the need for new approaches to treat and prevent bacterial infections. UPEC strains establish reservoirs in the gut from which they are shed in the faeces, and can colonize the periurethral area or vagina and subsequently ascend through the urethra to the urinary tract, where they cause UTIs...
June 22, 2017: Nature
https://www.readbyqxmd.com/read/28596755/genetic-diversity-and-virulence-determinants-of-escherichia-coli-strains-isolated-from-patients-with-crohn-s-disease-in-spain-and-chile
#11
Sandra Céspedes, Waleska Saitz, Felipe Del Canto, Marjorie De la Fuente, Rodrigo Quera, Marcela Hermoso, Rául Muñoz, Daniel Ginard, Sam Khorrami, Jorge Girón, Rodrigo Assar, Ramón Rosselló-Mora, Roberto M Vidal
Adherent-invasive Escherichia coli (AIEC) strains are genetically variable and virulence factors for AIEC are non-specific. FimH is the most studied pathogenicity-related protein, and there have been few studies on other proteins, such as Serine Protease Autotransporters of Enterobacteriacea (SPATEs). The goal of this study is to characterize E. coli strains isolated from patients with Crohn's disease (CD) in Chile and Spain, and identify genetic differences between strains associated with virulence markers and clonality...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28592545/development-of-a-web-tool-for-escherichia-coli-subtyping-based-on-fimh-alleles
#12
Louise Roer, Veronika Tchesnokova, Rosa Allesøe, Mariya Muradova, Sujay Chattopadhyay, Johanne Ahrenfeldt, Martin C F Thomsen, Ole Lund, Frank Hansen, Anette M Hammerum, Evgeni Sokurenko, Henrik Hasman
The aim of this study was to construct a valid publicly available method for in silico fimH subtyping of Escherichia coli particularly suitable for differentiation of fine-resolution subgroups within clonal groups defined by standard multilocus sequence typing (MLST). FimTyper was constructed as a FASTA database containing all currently known fimH alleles. The software source code is publicly available at https://bitbucket.org/genomicepidemiology/fimtyper, the database is freely available at https://bitbucket...
August 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28584160/rapid-identification-of-different-escherichia-coli-sequence-type-131-clades
#13
Yasufumi Matsumura, Johann D D Pitout, Gisele Peirano, Rebekah DeVinney, Taro Noguchi, Masaki Yamamoto, Ryota Gomi, Tomonari Matsuda, Satoshi Nakano, Miki Nagao, Michio Tanaka, Satoshi Ichiyama
Escherichia coli sequence type 131 (ST131) is a pandemic clonal lineage that is responsible for the global increase in fluoroquinolone resistance and extended-spectrum-β-lactamase (ESBL) producers. The members of ST131 clade C, especially subclades C2 and C1-M27, are associated with ESBLs. We developed a multiplex conventional PCR assay with the ability to detect all ST131 clades (A, B, and C), as well as C subclades (C1-M27, C1-nM27 [C1-non-M27], and C2). To validate the assay, we used 80 ST131 global isolates that had been fully sequenced...
August 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28584139/genomic-analysis-of-multidrug-resistant-escherichia-coli-from-north-carolina-community-hospitals-ongoing-circulation-of-ctx-m-producing-st131-h30rx-and-st131-h30r1-strains
#14
Hajime Kanamori, Christian M Parobek, Jonathan J Juliano, James R Johnson, Brian D Johnston, Timothy J Johnson, David J Weber, William A Rutala, Deverick J Anderson
Escherichia coli sequence type 131 (ST131) predominates globally among multidrug-resistant (MDR) E. coli strains. We used whole-genome sequencing (WGS) to investigate 63 MDR E. coli isolates from 7 North Carolina community hospitals (2010 to 2015). Of these, 39 (62%) represented ST131, including 37 (95%) from the ST131-H30R subclone: 10 (27%) from its H30R1 subset and 27 (69%) from its H30Rx subset. ST131 core genomes differed by a median of 15 (range, 0 to 490) single-nucleotide variants (SNVs) overall versus only 7 within H30R1 (range, 3 to 12 SNVs) and 11 within H30Rx (range, 0 to 21)...
August 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28560183/outbreak-by-hypermucoviscous-klebsiella-pneumoniae-st11-isolates-with-carbapenem-resistance-in-a-tertiary-hospital-in-china
#15
Lingling Zhan, Shanshan Wang, Yinjuan Guo, Ye Jin, Jingjing Duan, Zhihao Hao, Jingnan Lv, Xiuqin Qi, Longhua Hu, Liang Chen, Barry N Kreiswirth, Rong Zhang, Jingye Pan, Liangxing Wang, Fangyou Yu
Hypervirulent and multidrug resistant Klebsiella pneumoniae strains pose a significant threat to the public health. In the present study, 21 carbapenem-resistant K. pneumoniae isolates (CRKP) were determined by the string test as hypermucoviscous K. pneumoniae (HMKP), with the prevalence of 15.0% (21/140) among CRKP, and 1.1% (21/1838) among all K. pneumoniae isolates. Among them, 7 (33.3%), and 1 (4.76%) isolate belonged to capsular serotype K20 and K2 respectively, while 13 (61.9%, 13/21) weren't successfully typed by capsular serotyping...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28552831/extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-isolated-from-healthy-humans-in-mexico-including-subclone-st131-b2-o25-h4-h30-rx
#16
Gerardo Cortés-Cortés, Patricia Lozano-Zarain, Carmen Torres, Carla Andrea Alonso, Ana María Ríos-Torres, Miguel Castañeda, Liliana López-Pliego, Armando Navarro, Rosa Del Carmen Rocha-Gracia
OBJECTIVES: The resistance mechanisms, molecular type and plasmid content of cefotaxime-resistant Escherichia coli isolated from faecal samples of healthy volunteers in Puebla, Mexico, were characterised. METHODS AND RESULTS: Cefotaxime-resistant E. coli were recovered from 11 (18%) of 60 healthy volunteers. The isolates (one per sample) were characterised as multidrug-resistant and phenotypically extended-spectrum β-lactamase (ESBL)-producing strains. Genes encoding resistance to β-lactams (blaCTX-M-15, blaCTX-M-14a, blaCTX-M-14b, blaOXA-1, blaTEM-1b), quinolones [aac(6')-Ib-cr, qnrB19], aminoglycosides [aac(3')-II] and tetracycline [tet(A), tet(B)] were detected among the 11 ESBL-producing E...
June 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28545489/cytotoxic-necrotizing-factor-1-cnf1-does-not-promote-e-coli-infection-in-a-murine-model-of-ascending-pyelonephritis
#17
Jason E Michaud, Kwang Sik Kim, William Harty, Matthew Kasprenski, Ming-Hsien Wang
BACKGROUND: Urinary tract infections (UTI) are among the most common and costly infections in both hospitalized and ambulatory patients. Uropathogenic E. coli (UPEC) represent the majority of UTI isolates and are a diverse group of bacteria that utilize a variety of virulence factors to establish infection of the genitourinary tract. The virulence factor cytotoxic necrotizing factor-1 (CNF1) is frequently expressed in clinical UPEC isolates. To date, there have been conflicting reports on the role of CNF1 in the pathogenesis of E...
May 25, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28531220/highly-conserved-type-1-pili-promote-enterotoxigenic-e-coli-pathogen-host-interactions
#18
Alaullah Sheikh, Rasheduzzaman Rashu, Yasmin Ara Begum, F Matthew Kuhlman, Matthew A Ciorba, Scott J Hultgren, Firdausi Qadri, James M Fleckenstein
Enterotoxigenic Escherichia coli (ETEC), defined by their elaboration of heat-labile (LT) and/or heat-stable (ST) enterotoxins, are a common cause of diarrheal illness in developing countries. Efficient delivery of these toxins requires ETEC to engage target host enterocytes. This engagement is accomplished using a variety of pathovar-specific and conserved E. coli adhesin molecules as well as plasmid encoded colonization factors. Some of these adhesins undergo significant transcriptional modulation as ETEC encounter intestinal epithelia, perhaps suggesting that they cooperatively facilitate interaction with the host...
May 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28527285/-genetic-diversity-of-extraintestinal-escherichia-coli-strains-producers-of-beta-lactamases-tem-shv-and-ctx-m-associated-with-healthcare
#19
Yasmin Varela, Beatriz Millán, María Araque
INTRODUCTION: There are few reports from Venezuela describing the genetic basis that sustains the pathogenic potential and phylogenetics of Escherichia coli extraintestinal strains isolated in health care units. OBJECTIVE: To establish the genetic diversity of extraintestinal E. coli strains producers of betalactamases TEM, SHV and CTX-M associated with healthcare. MATERIALS AND METHODS: We studied a collection of 12 strains of extraintestinal E...
June 1, 2017: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/28506090/rational-design-strategies-for-fimh-antagonists-new-drugs-on-the-horizon-for-urinary-tract-infection-and-crohn-s-disease
#20
REVIEW
Laurel K Mydock-McGrane, Thomas J Hannan, James W Janetka
The bacterial adhesin FimH is a virulence factor and an attractive therapeutic target for urinary tract infection (UTI) and Crohn's Disease (CD). Located on type 1 pili of uropathogenic E. coli (UPEC), the FimH adhesin plays an integral role in the pathogenesis of UPEC. Recent efforts have culminated in the development of small-molecule mannoside FimH antagonists that target the mannose-binding lectin domain of FimH, inhibiting its function and preventing UPEC from binding mannosylated host cells in the bladder, thereby circumventing infection...
July 2017: Expert Opinion on Drug Discovery
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