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https://www.readbyqxmd.com/read/28104383/virulence-traits-and-pathogenicity-of-uropathogenic-escherichia-coli-isolates-with-common-and-uncommon-o-serotypes
#1
Qingqing Gao, Debao Zhang, Zhengqin Ye, Xiaoping Zhu, Weixia Yang, Lanmei Dong, Song Gao, Xiufan Liu
Urinary tract infections (UTIs) are among the most common human diseases worldwide. This study aimed to collect uropathogenic Escherichia coli (UPEC) isolates from Jiangsu Province and obtain insights into the molecular epidemiology of UPEC in this region. The O serotypes, phylogenetic groups, and virulence factors of 183 UPEC isolates were determined. In this study, we isolated 51 UPEC isolates with common O serotypes including O1, O2, O4, O6, O7, O16, O18 and O75, as well as 35 of those with uncommonly encountered O serotypes including O8, O12, O15, O26, and O74...
January 16, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28099062/rectal-carriage-of-extended-spectrum-beta-lactamase-and-carbapenemase-producing-gram-negative-bacilli-in-intensive-care-units-in-tunisia
#2
Samia Hammami, Chaima Dahdeh, Kelthoum Mamlouk, Sana Ferjeni, Elaa Maamar, Zeineb Hamzaoui, Mabrouka Saidani, Salma Ghedira, Mohamed Houissa, Amin Slim, Ilhem Boutiba-Ben Boubaker
This study was conducted to evaluate the rate of fecal carriage of Gram-negative bacilli (GNB) resistant to third-generation cephalosporins (third GC) in patients hospitalized in the intensive care unit (ICU) of Charles Nicolle Hospital of Tunis and to identify the enzymatic mechanisms involved. From February to April 2014, rectal swabs were collected from all patients (n = 38) at admission and once weekly thereafter to identify acquisition. They were cultured on desoxycholate-lactose-agar plates supplemented with cefotaxime (2 mg/L)...
January 18, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28077674/population-structure-of-japanese-extraintestinal-pathogenic-escherichia-coli-and-its-relationship-with-antimicrobial-resistance
#3
Yasufumi Matsumura, Taro Noguchi, Michio Tanaka, Toru Kanahashi, Masaki Yamamoto, Miki Nagao, Shunji Takakura, Satoshi Ichiyama
OBJECTIVES: To define the population structure of extraintestinal pathogenic Escherichia coli (ExPEC) in Japan and its relationship with antimicrobial resistance and the major resistance mechanisms for fluoroquinolones and β-lactams, we designed a multicentre prospective study. METHODS: A total of 329 ExPEC isolates were collected at 10 Japanese acute-care hospitals during December 2014. We defined the clonal groups of ExPEC by fumC and fimH sequencing (CH typing)...
January 10, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28034696/molecular-characterization-of-salmonella-typhimurium-isolated-in-brazil-by-crispr-mvlst
#4
Fernanda Almeida, Marta Inês Cazentini Medeiros, Dália Dos Prazeres Rodrigues, Marc W Allard, Juliana Pfrimer Falcão
CRISPR-multi-locus virulence sequence typing (CRISPR-MVLST) was performed to type 92 S. Typhimurium strains isolated from humans and food sources between 1983 and 2013 in Brazil and assess the suitability of this methodology comparing it with PFGE already used for subtyping the same strains. Among the 92 S. Typhimurium strains studied, we identified 25 CRISPR1 alleles, 27 CRISPR2 alleles, 2 fimH alleles and 3 sseL alleles showing that the genetic variability is much higher in the CRISPRs loci than in the virulence genes...
December 26, 2016: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28012984/virulence-factors-and-antimicrobial-susceptibility-profile-of-extraintestinal-escherichia-coli-isolated-from-an-avian-colisepticemia-outbreak
#5
Jonas Fernandes Maciel, Letícia Beatriz Matter, Michele Martins Trindade, Giovana Camillo, Maristela Lovato, Sônia de Ávila Botton, Agueda Castagna de Vargas
In this study an avian colisepticemia outbreak was investigated. Two isolates from a chicken with colisepticemia were characterized for antimicrobial susceptibility and virulence factors profile. For this purpose 7 antimicrobial and 29 genes (fimH, hrlA/hek, iha, papC, sfa/focCD, tsh, mat, tia, gimB, ibeA, chuA, fyuA, ireA, iroN, irp2, iucD, sitD. chr., sitD. ep., iss, neuC, ompA, traT, astA, hlyA, sat, vat, pic, malX, cvi/cva) were tested. The outbreak happened in a hick chicken breeding located in the northwestern region of Rio Grande do Sul state in South of Brazil and caused 28...
December 21, 2016: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28004732/molecular-typing-and-virulence-analysis-of-multidrug-resistant-klebsiella-pneumoniae-clinical-isolates-recovered-from-egyptian-hospitals
#6
Reham Wasfi, Walid F Elkhatib, Hossam M Ashour
Klebsiella pneumonia infection rates have increased dramatically. Molecular typing and virulence analysis are powerful tools that can shed light on Klebsiella pneumonia infections. Whereas 77.7% (28/36) of clinical isolates indicated multidrug resistant (MDR) patterns, 50% (18/36) indicated carpabenem resistance. Gene prevalence for the AcrAB efflux pump (82.14%) was more than that of the mdtK efflux pump (32.14%) in the MDR isolates. FimH-1 and mrkD genes were prevalent in wound and blood isolates. FimH-1 gene was prevalent in sputum while mrkD gene was prevalent in urine...
December 22, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27986723/whole-genome-analysis-of-antimicrobial-resistant-and-extraintestinal-pathogenic-escherichia-coli-in-river-water
#7
Ryota Gomi, Tomonari Matsuda, Yasufumi Matsumura, Masaki Yamamoto, Michio Tanaka, Satoshi Ichiyama, Minoru Yoneda
: Contamination of surface waters by antimicrobial-resistant bacteria and pathogenic bacteria is a great concern. In this study, 531 Escherichia coli isolates obtained from the Yamato River (Japan) were evaluated phenotypically for resistance to 25 antimicrobials. Seventy-six (14.3%) were multidrug resistant (MDR), 66 (12.4%) were non-susceptible to one or two classes of agents, and 389 (73.3%) were susceptible. We performed whole-genome sequencing using Illumina technology on selected strains...
December 16, 2016: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27934822/distribution-of-virulence-determinants-among-antimicrobial-resistant-and-antimicrobial-susceptible-escherichia-coli-implicated-in-urinary-tract-infections
#8
Sam Stephenson, P D Brown
INTRODUCTION: Uropathogenic Escherichia coli (UPEC) rely on the correlation of virulence expression with antimicrobial resistance to persist and cause severe urinary tract infections (UTIs). OBJECTIVES: We assessed the virulence pattern and prevalence among UPEC strains susceptible and resistant to multiple antimicrobial classes. METHODS: A total of 174 non-duplicate UPEC strains from patients with clinically significant UTIs were analysed for susceptibility to aminoglycoside, antifolate, cephalosporin, nitrofuran and quinolone antibiotics for the production of extended-spectrum β-lactamases and for the presence of six virulence determinants encoding adhesins (afimbrial, Type 1 fimbriae, P and S-fimbriae) and toxins (cytotoxic necrotising factor and haemolysin)...
October 2016: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27899921/high-prevalence-of-%C3%AE-lactamase-and-plasmid-mediated-quinolone-resistance-genes-in-extended-spectrum-cephalosporin-resistant-escherichia-coli-from-dogs-in-shaanxi-china
#9
Xiaoqiang Liu, Haixia Liu, Yinqian Li, Caiju Hao
Objective: The aim of this study was to investigate the occurrence and molecular characterization of extended-spectrum β-lactamases (ESBL), plasmid-mediated AmpC β-lactamase (pAmpC) and carbapenemases as well as plasmid-mediated quinolone-resistant (PMQR) among extended-spectrum cephalosporin-resistant (ESC-R) Escherichia coli from dogs in Shaanxi province in China. Methods: A total of 40 ESC-R Escherichia coli selected from 165 Extraintestinal pathogenic E. coli (ExPEC) isolated from dogs were screened and characterized for the genes encoding for the ESBLs, pAmpC, carbapenemases and PMQR genes by PCR and sequencing...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27843814/dimeric-and-trimeric-fusion-proteins-generated-with-fimbrial-adhesins-of-uropathogenic-escherichia-coli
#10
Víctor M Luna-Pineda, Juan Pablo Reyes-Grajeda, Ariadnna Cruz-Córdova, Zeus Saldaña-Ahuactzi, Sara A Ochoa, Carmen Maldonado-Bernal, Vicenta Cázares-Domínguez, Leticia Moreno-Fierros, José Arellano-Galindo, Rigoberto Hernández-Castro, Juan Xicohtencatl-Cortes
Urinary tract infections (UTIs) are associated with high rates of morbidity and mortality worldwide, and uropathogenic Escherichia coli (UPEC) is the main etiologic agent. Fimbriae assembled on the bacterial surface are essential for adhesion to the urinary tract epithelium. In this study, the FimH, CsgA, and PapG adhesins were fused to generate biomolecules for use as potential target vaccines against UTIs. The fusion protein design was generated using bioinformatics tools, and template fusion gene sequences were synthesized by GenScript in the following order fimH-csgA-papG-fimH-csgA (fcpfc) linked to the nucleotide sequence encoding the [EAAAK]5 peptide...
2016: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/27816993/adhesion-of-escherichia-coli-under-flow-conditions-reveals-potential-novel-effects-of-fimh-mutations
#11
T Feenstra, M S Thøgersen, E Wieser, A Peschel, M J Ball, R Brandes, S C Satchell, T Stockner, F M Aarestrup, A J Rees, R Kain
FimH-mediated adhesion of Escherichia coli to bladder epithelium is a prerequisite for urinary tract infections. FimH is also essential for blood-borne bacterial dissemination, but the mechanisms are poorly understood. The purpose of this study was to assess the influence of different FimH mutations on bacterial adhesion using a novel adhesion assay, which models the physiological flow conditions bacteria are exposed to. We introduced 12 different point mutations in the mannose binding pocket of FimH in an E...
November 5, 2016: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/27810882/alternate-thermoregulation-and-functional-binding-of-escherichia-coli-type-1-fimbriae-in-environmental-and-animal-isolates
#12
Jacqueline Marshall, Yannick Rossez, Geoffrey Mainda, David L Gally, Tim J Daniell, Nicola J Holden
Type 1 fimbriae (T1F) are well characterised cell surface organelles expressed by Escherichia coli and required for adherence to mannosylated host tissue. They satisfy molecular Koch's postulates as a virulence determinant and a host-adapted role has been reinforced by reports that T1F expression is repressed at submammalian temperatures. Analysis of a group of 136 environmental and animal E. coli isolates that express T1F at 37°C showed that 28% are also capable of expression at 20°C, in a phase variable manner...
November 2016: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/27711202/sphingosine-1-phosphate-activation-of-egfr-as-a-novel-target-for-meningitic-escherichia-coli-penetration-of-the-blood-brain-barrier
#13
Xiangru Wang, Ravi Maruvada, Andrew J Morris, Jun O Liu, Michael J Wolfgang, Dong Jae Baek, Robert Bittman, Kwang Sik Kim
Central nervous system (CNS) infection continues to be an important cause of mortality and morbidity, necessitating new approaches for investigating its pathogenesis, prevention and therapy. Escherichia coli is the most common Gram-negative bacillary organism causing meningitis, which develops following penetration of the blood-brain barrier (BBB). By chemical library screening, we identified epidermal growth factor receptor (EGFR) as a contributor to E. coli invasion of the BBB in vitro. Here, we obtained the direct evidence that CNS-infecting E...
October 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/27689912/antivirulence-c-mannosides-as-antibiotic-sparing-oral-therapeutics-for-urinary-tract-infections
#14
Laurel Mydock-McGrane, Zachary Cusumano, Zhenfu Han, Jana Binkley, Maria Kostakioti, Thomas Hannan, Jerome S Pinkner, Roger Klein, Vasilios Kalas, Jan Crowley, Nigam P Rath, Scott J Hultgren, James W Janetka
Gram-negative uropathogenic Escherichia coli (UPEC) bacteria are a causative pathogen of urinary tract infections (UTIs). Previously developed antivirulence inhibitors of the type 1 pilus adhesin, FimH, demonstrated oral activity in animal models of UTI but were found to have limited compound exposure due to the metabolic instability of the O-glycosidic bond (O-mannosides). Herein, we disclose that compounds having the O-glycosidic bond replaced with carbon linkages had improved stability and inhibitory activity against FimH...
October 27, 2016: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/27507827/glycomics-for-microbes-and-microbiologists
#15
Peter N Lipke
The recent article "Lectin-Glycan Interaction Network-Based Identification of Host Receptors of Microbial Pathogenic Adhesins" by Ielasi et al. describes a new development in microbial carbohydrate analysis [Ielasi FS, Alioscha-Perez M, Donohue D, Claes S, Sahli H, Schols D, Willaert RG, mBio 7(4):e00584-16, 2016, http://dx.doi.org/10.1128/mbio.00584-16]. Specific carbohydrate ligands have been identified from the patterns of lectin binding to oligosaccharides printed on a chip. The new technique links the output to a comprehensive glycan database and offers a number of data visualization options...
2016: MBio
https://www.readbyqxmd.com/read/27461240/use-of-flagellin-and-cholera-toxin-as-adjuvants-in-intranasal-vaccination-of-mice-to-enhance-protective-immune-responses-against-uropathogenic-escherichia-coli-antigens
#16
Mohammad Reza Asadi Karam, Mehri Habibi, Saeid Bouzari
Urinary tract infections (UTIs) caused by Uropathogenic Escherichia coli (UPEC) are among the most common infections in human. Antibiotics are common therapy for UTIs, but increase in antibiotic resistance will complicate future treatment of the infections, making the development of an efficacious UTI vaccine more urgent. In this study, we have evaluated intranasally the efficacy of FliC and FimH antigens of UPEC in different vaccine formulations with and without cholera toxin (CT) adjuvant. Immunization of mice with FliC in fusion form or admixed with FimH elicited higher levels of serum, mucosal and cell-mediated responses than FimH alone...
September 2016: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/27406561/lectin-glycan-interaction-network-based-identification-of-host-receptors-of-microbial-pathogenic-adhesins
#17
Francesco S Ielasi, Mitchel Alioscha-Perez, Dagmara Donohue, Sandra Claes, Hichem Sahli, Dominique Schols, Ronnie G Willaert
UNLABELLED: The first step in the infection of humans by microbial pathogens is their adherence to host tissue cells, which is frequently based on the binding of carbohydrate-binding proteins (lectin-like adhesins) to human cell receptors that expose glycans. In only a few cases have the human receptors of pathogenic adhesins been described. A novel strategy-based on the construction of a lectin-glycan interaction (LGI) network-to identify the potential human binding receptors for pathogenic adhesins with lectin activity was developed...
2016: MBio
https://www.readbyqxmd.com/read/27405093/identification-of-new-resistance-mechanisms-in-escherichia-coli-against-apidaecin-1b-using-quantitative-gel-and-lc-ms-based-proteomics
#18
Rico Schmidt, Andor Krizsan, Daniela Volke, Daniel Knappe, Ralf Hoffmann
Bacteria have acquired resistance mechanisms to overcome antibiotic treatments, triggering major concerns about the return of epidemic infections. Antimicrobial peptides identified in insects, animals, and plants represent a huge pool of promising lead structures that can be further developed for medical applications. Short proline-rich antimicrobial peptides (PrAMPs) have gained much attention due to their clinically interesting activity spectrum, serum protease stability, efficacy in murine infection models, and low adverse effects...
August 5, 2016: Journal of Proteome Research
https://www.readbyqxmd.com/read/27371583/evidence-of-naturalized-stress-tolerant-strains-of-escherichia-coli-in-municipal-wastewater-treatment-plants
#19
Shuai Zhi, Graham Banting, Qiaozhi Li, Thomas A Edge, Edward Topp, Mykola Sokurenko, Candis Scott, Shannon Braithwaite, Norma J Ruecker, Yutaka Yasui, Tim McAllister, Linda Chui, Norman F Neumann
UNLABELLED: Escherichia coli has been proposed to have two habitats-the intestines of mammals/birds and the nonhost environment. Our goal was to assess whether certain strains of E. coli have evolved toward adaptation and survival in wastewater. Raw sewage samples from different treatment plants were subjected to chlorine stress, and ∼59% of the surviving E. coli strains were found to contain a genetic insertion element (IS30) located within the uspC-flhDC intergenic region. The positional location of the IS30 element was not observed across a library of 845 E...
September 15, 2016: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27367677/subinhibitory-concentrations-of-allicin-decrease-uropathogenic-escherichia-coli-upec-biofilm-formation-adhesion-ability-and-swimming-motility
#20
Xiaolong Yang, Kaihui Sha, Guangya Xu, Hanwen Tian, Xiaoying Wang, Shanze Chen, Yi Wang, Jingyu Li, Junli Chen, Ning Huang
Uropathogenic Escherichia coli (UPEC) biofilm formation enables the organism to avoid the host immune system, resist antibiotics, and provide a reservoir for persistent infection. Once the biofilm is established, eradication of the infection becomes difficult. Therefore, strategies against UPEC biofilm are urgently required. In this study, we investigated the effect of allicin, isolated from garlic essential oil, on UPEC CFT073 and J96 biofilm formation and dispersal, along with its effect on UPEC adhesion ability and swimming motility...
June 29, 2016: International Journal of Molecular Sciences
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