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https://www.readbyqxmd.com/read/29209300/molecular-characteristics-of-st1193-clone-among-phylogenetic-group-b2-non-st131-fluoroquinolone-resistant-escherichia-coli
#1
Jing Wu, Fangjun Lan, Yanfang Lu, Qingwen He, Bin Li
Objectives: Sequence type 1193 is emerging as a new, virulent and resistant lineage among fluoroquinolone resistant Escherichia coli (FQrE. coli). In this study, we investigated the prevalence and molecular characteristics of this clone isolated from a Chinese university hospital. Methods: 73 phylogenetic group B2-FQr-non-ST131 isolates were collected from August 2014 and August 2015 at a Chinese university hospital. Isolates were screened for ST1193 by multilocus sequence typing. E. coli ST1193 then underwent lactose fermentation determination, susceptibility testing, virulence genotyping, PCR-based O typing, pulsed-field gel electrophoresis (PFGE) and FQr mechanism analysis...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29199378/distribution-of-pathogenicity-island-markers-and-virulence-factors-in-new-phylogenetic-groups-of-uropathogenic-escherichia-coli-isolates
#2
Akram Najafi, Mojtaba Hasanpour, Azam Askary, Masoud Aziemzadeh, Najmeh Hashemi
The present study was aimed at investigating the relationship between the new Clermont's phylogenetic groups, virulence factors, and pathogenicity island markers (PAIs) among uropathogenic Escherichia coli (UPEC) in Iran. This cross-sectional study was carried out on 140 UPEC isolates collected from patients with urinary tract infections in Bushehr, Iran. All isolates were subjected to phylogenetic typing using a new quadruplex-PCR method. The presence of PAI markers and virulence factors in UPEC strains was evaluated by multiplex PCR...
December 3, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/29181009/crispri-induced-suppression-of-fimbriae-gene-fimh-of-a-uropathogenic-escherichia-coli-an-approach-to-inhibit-microbial-biofilms
#3
Azna Zuberi, Nayeem Ahmad, Asad U Khan
Urinary tract infection (UTI) is one the common infections caused by the recalcitrant nature of biofilms, developed after the pathogen has adhered to the inner lining of the urinary tract. Although significant research has been made in recent years to control these types of infection, but as of yet, no approach has sufficiently been able to reduce the prevalence of UTIs. The main objective of this study was to prevent UTIs through targeting the fimH gene, which is the major virulent factor responsible for biofilm formation...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29180452/conformational-switch-of-the-bacterial-adhesin-fimh-in-the-absence-of-the-regulatory-domain-engineering-a-minimalistic-allosteric-system
#4
Said Rabbani, Brigitte Fiege, Deniz Eris, Marleen Silbermann, Roman Peter Jakob, Giulio Navarra, Timm Maier, Beat Ernst
For many biological processes such as ligand binding, enzymatic catalysis, or protein folding, allosteric regulation of protein conformation and dynamics is fundamentally important. One example is the bacterial adhesin FimH, where the C-terminal pilin domain exerts negative allosteric control over binding of the N-terminal lectin domain to mannosylated ligands on host cells. When the lectin and pilin domains are separated under shear stress, the FimH-ligand interaction switches in a so-called catch-bond mechanism from low to high affinity state...
November 27, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29153063/-investigation-of-various-virulence-factors-of-klebsiella-pneumoniae-strains-isolated-from-nosocomial-infections
#5
Halit Kuş, Uğur Arslan, Hatice Türk Dağı, Duygu Fındık
Klebsiella pneumoniae is an opportunistic pathogen that commonly affects immunosuppressed patients and causes nosocomial infections. K.pneumoniae has a variety of virulence factors, especially capsule polysaccharide, hypermucoviscosity (HV), fimbriae, toxins and determinants for iron acquisition. The aim of this study was to detect the virulence factors in K.pneumoniae strains isolated from nosocomial infections in two years. Fifty three K.pneumoniae strains isolated from the samples of patients with nosocomial infections in the Medical Microbiology Laboratory of Selcuk University Faculty of Medicine Hospital between 2011 and 2013 were included in the study...
October 2017: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/29141957/adherent-invasive-escherichia-coli-in-inflammatory-bowel-disease
#6
REVIEW
Carolina Palmela, Caroline Chevarin, Zhilu Xu, Joana Torres, Gwladys Sevrin, Robert Hirten, Nicolas Barnich, Siew C Ng, Jean-Frederic Colombel
Intestinal microbiome dysbiosis has been consistently described in patients with IBD. In the last decades, Escherichia coli, and the adherent-invasive E coli (AIEC) pathotype in particular, has been implicated in the pathogenesis of IBD. Since the discovery of AIEC, two decades ago, progress has been made in unravelling these bacteria characteristics and its interaction with the gut immune system. The mechanisms of adhesion of AIEC to intestinal epithelial cells (via FimH and cell adhesion molecule 6) and its ability to escape autophagy when inside macrophages are reviewed here...
November 15, 2017: Gut
https://www.readbyqxmd.com/read/29115711/linear-precision-glycomacromolecules-with-varying-interligand-spacing-and-linker-functionalities-binding-to-concanavalin-a-and-the-bacterial-lectin-fimh
#7
Sinaida Igde, Susanna Röblitz, Anne Müller, Katharina Kolbe, Sophia Boden, Claudia Fessele, Thisbe K Lindhorst, Marcus Weber, Laura Hartmann
A series of precision glycomacromolecules is prepared following previously established solid phase synthesis allowing for controlled variations of interligand spacing and the overall number of carbohydrate ligands. In addition, now also different linkers are installed between the carbohydrate ligand and the macromolecular scaffold. The lectin binding behavior of these glycomacromolecules is then evaluated in isothermal titration calorimetry (ITC) and kinITC experiments using the lectin Concanavalin A (Con A) in its dimeric and tetrameric form...
November 8, 2017: Macromolecular Bioscience
https://www.readbyqxmd.com/read/29055550/the-phylogenetic-group-antimicrobial-susceptibility-and-virulence-genes-of-escherichia-coli-from-clinical-bovine-mastitis
#8
Dexian Zhang, Zehui Zhang, Chengcheng Huang, Xiang Gao, Zhuang Wang, Yaochuan Liu, Chunlian Tian, Wei Hong, Shengli Niu, Mingchun Liu
Bovine mastitis is still a central problem on dairy farms despite control programs, and Escherichia coli is a crucial pathogen during the development of bovine mastitis. The virulence genes, antimicrobial susceptibility, and mortality of mice infected with different E. coli isolates from bovine mastitis were determined in this study. According to the presence of the specific genes chuA, yjaA, and TspE4.C2, these isolates mainly belonged to 2 different groups: group A (47/79) and group B1 (22/79). The ompC gene was detected in all the isolates, followed by fimH (89...
October 18, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/29054868/conditional-function-of-autoaggregative-protein-cah-and-common-cah-mutations-in-shiga-toxin-producing-escherichia-coli
#9
Michelle Qiu Carter, Maria T Brandl, Indira T Kudva, Robab Katani, Matthew R Moreau, Vivek Kapur
Cah is a calcium-binding autotransporter protein involved in autoaggregation and biofilm formation. Although cah is widespread in Shiga toxin-producing Escherichia coli (STEC), we detected mutations in cah at a frequency of 31.3% in this pathogen. In STEC O157:H7 super-shedder strain SS17, a large deletion results in a smaller coding sequence, lacking the C-terminal 71 amino acids compared with Cah in STEC O157:H7 strain EDL933. We examined the function of Cah in biofilm formation and host colonization to better understand selective pressures for cah mutations...
October 20, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29031453/whole-genome-comparison-of-urinary-pathogenic-escherichia-coli-and-faecal-isolates-of-uti-patients-and-healthy-controls
#10
Karen Leth Nielsen, Marc Stegger, Kristoffer Kiil, Paul A Godfrey, Michael Feldgarden, Berit Lilje, Paal S Andersen, Niels Frimodt-Møller
The faecal flora is a common reservoir for urinary tract infection (UTI), and Escherichia coli (E. coli) is frequently found in this reservoir without causing extraintestinal infection. We investigated these E. coli reservoirs by whole-genome sequencing a large collection of E. coli from healthy controls (faecal), who had never previously had UTI, and from UTI patients (faecal and urinary) sampled from the same geographical area. We compared MLST types, phylogenetic relationship, accessory genome content and FimH type between patient and control faecal isolates as well as between UTI and faecal-only isolates, respectively...
September 14, 2017: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/29024820/coating-of-silicone-with-mannoside-pamam-dendrimers-to-enhance-formation-of-non-pathogenic-escherichia-coli-biofilms-against-colonization-of-uropathogens
#11
Zhiling Zhu, Fei Yu, Haoqing Chen, Jun Wang, Analette I Lopez, Quan Chen, Siheng Li, Yuyu Long, Rabih O Darouiche, Richard A Hull, Lijuan Zhang, Chengzhi Cai
Bacterial interference using non-pathogenic Escherichia coli 83972 is a novel strategy for preventing catheter-associated urinary tract infection (CAUTI). Crucial to the success of this strategy is to establish a high coverage and stable biofilm of the non-pathogenic bacteria on the catheter surface. However, this non-pathogenic strain is sluggish to form biofilms on silicone as the most widely used material for urinary catheters. We have addressed this issue by modifying the silicone catheter surfaces with mannosides that promote the biofilm formation, but the stability of the non-pathogenic biofilms challenged by uropathogens over long-term remains a concern...
December 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28987619/genetic-diversity-phylogroup-distribution-and-virulence-gene-profile-of-pks-positive-escherichia-coli-colonizing-human-intestinal-polyps
#12
Meysam Sarshar, Daniela Scribano, Massimiliano Marazzato, Cecilia Ambrosi, Maria Rita Aprea, Marta Aleandri, Annamaria Pronio, Catia Longhi, Mauro Nicoletti, Carlo Zagaglia, Anna Teresa Palamara, Maria Pia Conte
Some Escherichia coli strains of phylogroup B2 harbor a (pks) pathogenicity island that encodes a polyketide-peptide genotoxin called colibactin. It causes DNA double-strand breaks and megalocytosis in eukaryotic cells and it may contribute to cancer development. Study of bacterial community that colonizes the adenomatous polyp lesion, defined as precancerous lesions, could be helpful to assess if such pathogenic bacteria possess a role in the polyp progression to cancer. In this cross-sectional study, a total of 1500 E...
October 5, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28976770/level-of-fimbriation-alters-the-adhesion-of-escherichia-coli-bacteria-to-interfaces
#13
Ryan B McLay, Hang N Nguyen, Yuly Andrea Jaimes-Lizcano, Narendra K Dewangan, Simone Alexandrova, Debora F Rodrigues, Patrick C Cirino, Jacinta C Conrad
Adhesion of bacteria to interfaces is the first step in pathogenic infection, in biofilm formation, and in bioremediation of oil spills and other pollutants. Bacteria use a variety of surface structures to promote interfacial adhesion, with the level of expression of these structures varying in response to local conditions and environmental signals. Here, we investigated how overexpression of type 1 fimbriae, one such appendage, modifies the ability of Escherichia coli to adhere to solid substrates, via biofilm formation and yeast agglomeration, and to oil/water interfaces, via a microbial adhesion to hydrocarbon assay...
October 17, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28915364/nosocomial-multidrug-resistant-klebsiella-pneumoniae-strains-isolated-from-mexico-city-produce-robust-biofilms-on-abiotic-surfaces-but-not-on-human-lung-cells
#14
Martha Lorena Ostria-Hernandez, Karla Cecilia Juárez-de la Rosa, Patricia Arzate-Barbosa, Antonino Lara-Hernández, Fuminori Sakai, J Antonio Ibarra, Graciela Castro-Escarpulli, Jorge E Vidal
BACKGROUND: Klebsiella pneumoniae (Kpn) strains are a leading cause of hospital-acquired infections, including ventilator-associated pneumonia. Resistance to antibiotics, biofilm formation, and the production of certain fimbriae play an important role in the pathogenesis. AIM: We investigated the genetic relatedness, antibiotic resistance, virulence potential, and ability to form biofilms of Kpn strains isolated from hospital-acquired infections (n = 76). Strains were isolated at three major hospitals serving the largest metropolitan urban area in Mexico City, Mexico...
September 15, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28808163/role-for-fimh-in-extraintestinal-pathogenic-escherichia-coli-invasion-and-translocation-through-the-intestinal-epithelium
#15
Nina M Poole, Sabrina I Green, Anubama Rajan, Luz E Vela, Xi-Lei Zeng, Mary K Estes, Anthony W Maresso
The translocation of bacteria across the intestinal epithelium of immunocompromised patients can lead to bacteremia and life-threatening sepsis. Extraintestinal pathogenic Escherichia coli (ExPEC), so named because this pathotype infects tissues distal to the intestinal tract, is a frequent cause of such infections, is often multidrug resistant, and chronically colonizes a sizable portion of the healthy population. Although several virulence factors and their roles in pathogenesis are well described for ExPEC strains that cause urinary tract infections and meningitis, they have not been linked to translocation through intestinal barriers, a fundamentally distant yet important clinical phenomenon...
November 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28770174/the-novel-type-1-fimbriae-fimh-receptor-calreticulin-plays-a-role-in-salmonella-host-specificity
#16
Krzysztof Grzymajlo, Maciej Ugorski, Jaroslaw Suchanski, Anna E Kedzierska, Rafal Kolenda, Anna Jarzab, Agnieszka Biernatowska, Peter Schierack
It was suggested that minor differences in the structure of FimH are most likely associated with differences in its adhesion specificities and may determine the tropism of various Salmonella serovars to different species and tissues. We have recently shown that FimH adhesins from host-adapted serovars, e.g., Salmonella Choleraesuis (SCh), bind to other glycoprotein receptors compared to FimH from host-unrestricted Salmonella Enteritidis (SE). Here we identify porcine calreticulin expressed by swine intestinal cells as a host-specific receptor for SCh FimH adhesin, suggesting that such an interaction may contribute to SCh host specificity...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28762293/the-potential-of-fimh-as-a-novel-therapeutic-target-for-the-treatment-of-crohn-s-disease
#17
REVIEW
Adeline Sivignon, Julie Bouckaert, Julien Bernard, Sebastien G Gouin, Nicolas Barnich
Crohn's disease (CD) is a life-long chronic disorder characterized by intestinal inflammation. Current treatments for CD are directed towards abnormal immune responses rather than the intestinal bacteria that trigger intestinal inflammation. Areas covered: Adherent-Invasive Escherichia coli (AIEC) bacteria abnormally colonize the ileal mucosa in a subgroup of CD patients. They can promote or perpetuate chronic inflammation and are therefore an interesting therapeutic target. Various strategies that target these E...
September 2017: Expert Opinion on Therapeutic Targets
https://www.readbyqxmd.com/read/28760455/association-between-virulence-profile-biofilm-formation-and-phylogenetic-groups-of-escherichia-coli-causing-urinary-tract-infection-and-the-commensal-gut-microbiota-a-comparative-analysis
#18
Zahra Hashemizadeh, Davood Kalantar-Neyestanaki, Shahla Mansouri
Variety of virulence factors are involved in the pathogenicity of Escherichia coli, the common cause of the urinary tract infections (UTIs). The aim of this study was to determine some virulence factors involved in the pathogenicity and the phylogenetic grouping of E. coli from UTIs compared with the E. coli isolates from gut microbiota (fecal flora). The isolates were tested for biofilm formation, haemagglutination, cell surface hydrophobicity (CSH), hemolysin production, phylogenetic grouping and the distribution of 6 known virulence genes...
September 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28723213/dual-functional-core-shell-fluorescent-ag2s-carbon-nanostructure-for-selective-assay-of-e-coli-o157-h7-and-bactericidal-treatment
#19
Ning Wang, Xing Wei, An-Qi Zheng, Ting Yang, Ming-Li Chen, Jian-Hua Wang
A dual functional fluorescent core-shell Ag2S@Carbon nanostructure is prepared by a hydrothermally assisted multi-amino synthesis approach with folic acid (FA), polyethylenimine (PEI), and mannoses (Mans) as carbon and nitrogen sources (FA-PEI-Mans-Ag2S nanocomposite shortly as Ag2S@C). The nanostructure exhibits strong fluorescent emission at λex/λem = 340/450 nm with a quantum yield of 12.57 ± 0.52%. Ag2S@C is bound to E. coli O157:H7 via strong interaction with the Mans moiety in Ag2S@C with FimH proteins on the fimbriae tip in E...
March 24, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28683322/stromal-cell-derived-factor-1-mediates-immune-cell-attraction-upon-urinary-tract-infection
#20
Batya Isaacson, Tehila Hadad, Ariella Glasner, Chamutal Gur, Zvi Granot, Gilad Bachrach, Ofer Mandelboim
Urinary tract infection (UTI) is the most common type of bacterial infection in humans. Fifty percent of all women will experience at least one UTI in their lifetime, with uropathogenic Escherichia coli (UPEC) accounting for 80% of reported cases. UTI evokes a complex, well-timed immune response that is crucial for bacterial clearance. The majority of immune cells participating in the immune response are absent from the healthy bladder, and the mechanisms used to recruit them upon UTI are not fully understood...
July 5, 2017: Cell Reports
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