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https://www.readbyqxmd.com/read/28531220/highly-conserved-type-1-pili-promote-enterotoxigenic-e-coli-pathogen-host-interactions
#1
Alaullah Sheikh, Rasheduzzaman Rashu, Yasmin Ara Begum, F Matthew Kuhlman, Matthew A Ciorba, Scott J Hultgren, Firdausi Qadri, James M Fleckenstein
Enterotoxigenic Escherichia coli (ETEC), defined by their elaboration of heat-labile (LT) and/or heat-stable (ST) enterotoxins, are a common cause of diarrheal illness in developing countries. Efficient delivery of these toxins requires ETEC to engage target host enterocytes. This engagement is accomplished using a variety of pathovar-specific and conserved E. coli adhesin molecules as well as plasmid encoded colonization factors. Some of these adhesins undergo significant transcriptional modulation as ETEC encounter intestinal epithelia, perhaps suggesting that they cooperatively facilitate interaction with the host...
May 22, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28527285/-genetic-diversity-of-extraintestinal-escherichia-coli-strains-producers-of-beta-lactamases-tem-shv-and-ctx-m-associated-with-healthcare
#2
Yasmin Varela, Beatriz Millán, María Araque
INTRODUCTION: There are few reports from Venezuela describing the genetic basis that sustains the pathogenic potential and phylogenetics of Escherichia coli extraintestinal strains isolated in health care units. OBJECTIVE: To establish the genetic diversity of extraintestinal E. coli strains producers of betalactamases TEM, SHV and CTX-M associated with healthcare. MATERIALS AND METHODS: We studied a collection of 12 strains of extraintestinal E...
June 1, 2017: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/28506090/rational-design-strategies-for-fimh-antagonists-new-drugs-on-the-horizon-for-urinary-tract-infection-and-crohn-s-disease
#3
Laurel K Mydock-McGrane, Thomas J Hannan, James W Janetka
The bacterial adhesin FimH is a virulence factor and an attractive therapeutic target for urinary tract infection (UTI) and Crohn's Disease (CD). Located on type 1 pili of uropathogenic E. coli (UPEC), the FimH adhesin plays an integral role in the pathogenesis of UPEC. Recent efforts have culminated in the development of small-molecule mannoside FimH antagonists that target the mannose-binding lectin domain of FimH, inhibiting its function and preventing UPEC from binding mannosylated host cells in the bladder, thereby circumventing infection...
May 16, 2017: Expert Opinion on Drug Discovery
https://www.readbyqxmd.com/read/28490179/-living-dynamics-of-filamentous-bacteria-on-an-adherent-surface-under-hydrodynamic-exposure
#4
Justin P Jahnke, Austin M Smith, Nicole E Zander, Victoria Wiedorn, Kenneth E Strawhecker, Jessica L Terrell, Dimitra N Stratis-Cullum, Xuanhong Cheng
The potential advantages of cell-based biohybrid devices over conventional nonliving systems drive the interest to control the behavior of the underlying biological cells in microdevices. Here, the authors studied how shear influenced the geometry and elongation of fimbriated filaments on affinity substrates. The cells were engineered to express FimH, which binds to mannose with a high affinity. A microfluidic channel was functionalized with RNAse B, which is rich in mannose residues, and the device was used to control the hydrodynamic force on live Escherichia coli under filamentous growth...
May 10, 2017: Biointerphases
https://www.readbyqxmd.com/read/28478807/aqueous-extract-from-orthosiphon-stamineus-leaves-prevents-bladder-and-kidney-infection-in-mice
#5
S Sarshar, S Brandt, M R Asadi Karam, M Habibi, S Bouzari, M Lechtenberg, U Dobrindt, X Qin, F M Goycoolea, A Hensel
BACKGROUND: Extracts from the leaves of Orthosiphon stamineus are used in phytotherapy for treatment of uncomplicated urinary tract infections. PURPOSES: Evaluation of an aqueous extract against infection with uropathogenic Escherichia coli in vivo; investigation of underlying microbiological mechanisms. STUDY DESIGN: In vivo studies in mice and in vitro investigations on cytotoxicity, antiadhesive potential, influence on bacterial gene expression and quorum sensing...
May 15, 2017: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/28471659/urinary-tract-infection-which-conformation-of-the-bacterial-lectin-fimh-is-therapeutically-relevant
#6
Katharina Mayer, Deniz Eris, Oliver Schwardt, Christoph P Sager, Said Rabbani, Simon Kleeb, Beat Ernst
Frequent antibiotic treatment of urinary tract infections has resulted in the emergence of antimicrobial resistance, necessitating alternative treatment options. One such approach centers around FimH antagonists that block the bacterial adhesin FimH, which would otherwise mediate binding of uropathogenic Escherichia coli to the host urothelium to trigger the infection. Although the FimH lectin can adopt three distinct conformations, the evaluation of FimH antagonists has mainly been performed with a truncated construct of FimH locked in one particular conformation...
May 11, 2017: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28459221/association-between-virulence-profile-and-fluoroquinolone-resistance-in-escherichia-coli-isolated-from-dogs-and-cats-in-china
#7
Xiaoqiang Liu, Haixia Liu, Yinqian Li, Caiju Hao
INTRODUCTION: Escherichia coli is not only a commensal organism in humans and animals, but also a causative agent of diarrhea and extraintestinal infections. Information about the relationship between population structure, virulence gene profiles, and fluoroquinolone resistance of E. coli in dogs and cats in China is limited. METHODOLOGY: A total of 174 pathogenic and commensal E. coli isolates were evaluated in terms of phylogenetic group, virulence gene profile, sequence types (STs), and fluoroquinolone susceptibility...
April 30, 2017: Journal of Infection in Developing Countries
https://www.readbyqxmd.com/read/28432491/anti-biofilm-activity-of-polyazolidinammonium-modified-with-iodine-hydrate-ions-against-microbial-biofilms-of-uropathogenic-coliform-bacteria
#8
O V Nechaeva, E I Tikhomirova, D A Zayarsky, N V Bespalova, E V Glinskaya, N F Shurshalova, B M Al Bayati, A I Babailova
The dynamics of microbial biofilm formation by standard strain and by clinical strains of uropathogenic coliform bacteria was investigated in vitro and the effect of sublethal concentrations of the polymer compound polyazolidinammonium modified with iodine hydrate ions on the initial stages of biofilm formation was assessed. Treatment of immunological plate wells with the polymeric compound prevented film formation, especially in case of clinical E. coli strain carrying FimH virulence gene.
April 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28405633/genomic-analysis-of-factors-associated-with-low-prevalence-of-antibiotic-resistance-in-extraintestinal-pathogenic-escherichia-coli-sequence-type-95-strains
#9
Craig M Stephens, Sheila Adams-Sapper, Manraj Sekhon, James R Johnson, Lee W Riley
Extraintestinal pathogenic Escherichia coli (ExPEC) strains belonging to multilocus sequence type 95 (ST95) are globally distributed and a common cause of infections in humans and domestic fowl. ST95 isolates generally show a lower prevalence of acquired antimicrobial resistance than other pandemic ExPEC lineages. We took a genomic approach to identify factors that may underlie reduced resistance. We fully assembled genomes for four ST95 isolates representing the four major fimH-based lineages within ST95 and also analyzed draft-level genomes from another 82 ST95 isolates, largely from the western United States...
March 2017: MSphere
https://www.readbyqxmd.com/read/28388949/characterization-and-virulence-clustering-analysis-of-extraintestinal-pathogenic-escherichia-coli-isolated-from-swine-in-china
#10
Yinchu Zhu, Wenyang Dong, Jiale Ma, Lvfeng Yuan, Hassan M A Hejair, Zihao Pan, Guangjin Liu, Huochun Yao
BACKGROUND: Swine extraintestinal pathogenic Escherichia coli (ExPEC) is an important pathogen that leads to economic and welfare costs in the swine industry worldwide, and is occurring with increasing frequency in China. By far, various virulence factors have been recognized in ExPEC. Here, we investigated the virulence genotypes and clonal structure of collected strains to improve the knowledge of phylogenetic traits of porcine ExPECs in China. RESULTS: We isolated 64 Chinese porcine ExPEC strains from 2013 to 14 in China...
April 8, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28369408/wgs-based-surveillance-of-third-generation-cephalosporin-resistant-escherichia-coli-from-bloodstream-infections-in-denmark
#11
Louise Roer, Frank Hansen, Martin Christen Frølund Thomsen, Jenny Dahl Knudsen, Dennis Schrøder Hansen, Mikala Wang, Jurgita Samulioniené, Ulrik Stenz Justesen, Bent L Røder, Helga Schumacher, Claus Østergaard, Leif Percival Andersen, Esad Dzajic, Turid Snekloth Søndergaard, Marc Stegger, Anette M Hammerum, Henrik Hasman
Objectives: To evaluate a genome-based surveillance of all Danish third-generation cephalosporin-resistant Escherichia coli (3GC-R Ec ) from bloodstream infections between 2014 and 2015, focusing on horizontally transferable resistance mechanisms. Methods: A collection of 552 3GC-R Ec isolates were whole-genome sequenced and characterized by using the batch uploader from the Center for Genomic Epidemiology (CGE) and automatically analysed using the CGE tools according to resistance profile, MLST, serotype and fimH subtype...
March 22, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28365752/high-frequency-of-b2-phylogroup-among-non-clonally-related-fecal-escherichia-coli-isolates-from-wild-boars-including-the-lineage-st131
#12
Carla Andrea Alonso, David González-Barrio, Francisco Ruiz-Fons, Laura Ruiz-Ripa, Carmen Torres
Wild boars are worldwide distributed mammals which population is increasing in many regions, like the Iberian Peninsula, leading to an increased exposition to humans. They are considered reservoirs of different zoonotic pathogens and have been postulated as potential vectors of antimicrobial-resistant (AMR) bacteria. This study aimed to determine the prevalence of antimicrobial resistance and phylogenetic distribution of Escherichia coli from wild boar feces. Antimicrobial resistance and integron content was genetically characterized and E...
March 1, 2017: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/28347271/the-st131-escherichia-coli-h22-subclone-from-human-intestinal-microbiota-comparison-of-genomic-and-phenotypic-traits-with-those-of-the-globally-successful-h30-subclone
#13
Marie-Hélène Nicolas-Chanoine, Marie Petitjean, Azucena Mora, Noémie Mayer, Jean-Philippe Lavigne, Olivier Boulet, Véronique Leflon-Guibout, Jorge Blanco, Didier Hocquet
BACKGROUND: In 2006, we found healthy subjects carrying ST131 Escherichia coli in their intestinal microbiota consisting of two populations: a subdominant population of fluoroquinolone-resistant E. coli belonging to subclone H30 (H30-R or subclade C1), the current worldwide dominant ST131 subclone, and a dominant E. coli population composed of antibiotic-susceptible E. coli belonging to subclone H22 (clade B), the precursor of subclone H30. We sequenced the whole genome of fecal H22 strain S250, compared it to the genomes of ExPEC ST131 H30-Rx strain JJ1886 and commensal ST131 H41 strain SE15, sought the H22-H30 genomic differences in our fecal strains and assessed their phenotypic consequences...
March 27, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28339623/the-molecular-and-clinical-epidemiology-of-extended-spectrum-cephalosporin-and-carbapenem-resistant-enterobacteriaceae-at-4-us-pediatric-hospitals
#14
Danielle M Zerr, Scott J Weissman, Chuan Zhou, Matthew P Kronman, Amanda L Adler, Jessica E Berry, Jaipreet Rayar, Jeff Myers, Wren L Haaland, Carey-Ann D Burnham, Alexis Elward, Jason Newland, Rangaraj Selvarangan, Kaede V Sullivan, Theoklis Zaoutis, Xuan Qin
Objective.: In this report, we aim to describe the epidemiology of extended-spectrum cephalosporin-resistant (ESC-R) and carbapenem-resistant (CR) Enterobacteriaceae infections in children. Methods.: ESC-R and CR Enterobacteriaceae isolates from normally sterile sites of patients aged <22 years from 4 freestanding pediatric medical centers were collected along with the associated clinical data. Results.: The overall frequencies of ESC-R and CR isolates according to hospital over the 4-year study period ranged from 0...
January 19, 2017: Journal of the Pediatric Infectious Diseases Society
https://www.readbyqxmd.com/read/28289725/cooperation-of-adhesin-alleles-in-salmonella-host-tropism
#15
Leon De Masi, Min Yue, Changmin Hu, Alexey V Rakov, Shelley C Rankin, Dieter M Schifferli
Allelic combinations and host specificities for three fimbrial adhesins, FimH, BcfD, and StfH, were compared for 262 strains of Salmonella enterica serovar Newport, a frequent human and livestock pathogen. Like FimH, BcfD had two major alleles (designated A and B), whereas StfH had two allelic groups, each with two alleles (subgroup A1 and A2 and subgroup B1 and B2). The most prevalent combinations of FimH/BcfD/StfH alleles in S. Newport were A/A/A1 and B/B/B1. The former set was most frequently found in bovine and porcine strains, whereas the latter combination was most frequently found in environmental and human isolates...
March 2017: MSphere
https://www.readbyqxmd.com/read/28289030/antibiotic-prophylaxis-is-associated-with-subsequent-resistant-infections-in-children-with-an-initial-extended-spectrum-cephalosporin-resistant-enterobacteriaceae-infection
#16
Sibani Das, Amanda L Adler, Arianna Miles-Jay, Matthew P Kronman, Xuan Qin, Scott J Weissman, C A Burnham, Alexis Elward, Jason G Newland, Rangaraj Selvarangan, Kaede V Sullivan, Theoklis Zaoutis, Danielle M Zerr
The objective of this study was to assess the association between previous antibiotic use, particularly long-term prophylaxis, and the occurrence of subsequent resistant infections in children with index infections due to extended-spectrum-cephalosporin-resistant Enterobacteriaceae We also investigated the concordance of the index and subsequent isolates. Extended-spectrum-cephalosporin-resistant Escherichia coli and Klebsiella spp. isolated from normally sterile sites of patients aged <22 years were collected along with associated clinical data from four freestanding pediatric centers...
May 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28288685/ontology-based-literature-mining-of-e-coli-vaccine-associated-gene-interaction-networks
#17
Junguk Hur, Arzucan Özgür, Yongqun He
BACKGROUND: Pathogenic Escherichia coli infections cause various diseases in humans and many animal species. However, with extensive E. coli vaccine research, we are still unable to fully protect ourselves against E. coli infections. To more rational development of effective and safe E. coli vaccine, it is important to better understand E. coli vaccine-associated gene interaction networks. METHODS: In this study, we first extended the Vaccine Ontology (VO) to semantically represent various E...
March 14, 2017: Journal of Biomedical Semantics
https://www.readbyqxmd.com/read/28257756/bacteraemia-due-to-non-esbl-producing-escherichia-coli-o25b-h4-sequence-type-131-insights-into-risk-factors-clinical-features-and-outcomes
#18
Isabel Morales-Barroso, Lorena López-Cerero, José Molina, Mar Bellido, María Dolores Navarro, Lara Serrano, Verónica González-Galán, Julia Praena, Alvaro Pascual, Jesús Rodríguez-Baño
The epidemiology and outcomes of bloodstream infections (BSIs) caused by Escherichia coli ST131 isolates not producing extended-spectrum β-lactamases (ESBLs) are not well defined despite being more prevalent than ESBL-producers. In this study, risk factors and the impact on outcome of BSIs caused by non-ESBL-producing ST131 E. coli versus non-ST131 E. coli were investigated. A case-control study was performed in two tertiary centres to identify risk factors for ST131. Molecular methods were used to investigate all E...
April 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28257558/physiochemical-tuning-of-potent-e-coli-antiadhesives-by-microencapsulation-and-methylene-homologation
#19
Dimitri Alvarez Dorta, Thibaut Chalopin, Adeline Sivignon, Jerome De Ruyck, Tanya Dumych, Rostyslav Bilyy, David Deniaud, Nicolas Barnich, Julie Bouckaert, Sébastien G Gouin
Thiazolylaminomannosides (TazMan) are FimH antagonists with anti-adhesive potential against adherent-invasive Escherichia coli (AIEC) promoting gut inflammation in patients with Crohn's disease (CD). The lead TazMan is highly potent in vitro but shows limited in vivo efficiency probably due to low pH stability and water solubility. We recently developed a second generation of stable TazMan but the anti-adhesive effect was decreased compared to the first. Here, we report the co-crystal structure of the lead TazMan in FimH, revealing that the anomeric NH and the second thiazole moiety provide a positive H-bonding interaction with a trapped water molecule, and π-stacking with Tyrosine 48 of FimH, respectively...
March 3, 2017: ChemMedChem
https://www.readbyqxmd.com/read/28250938/mutation-of-tyr137-of-the-universal-escherichia-coli-fimbrial-adhesin-fimh-relaxes-the-tyrosine-gate-prior-to-mannose-binding
#20
Said Rabbani, Eva-Maria Krammer, Goedele Roos, Adam Zalewski, Roland Preston, Sameh Eid, Pascal Zihlmann, Martine Prévost, Marc F Lensink, Andrew Thompson, Beat Ernst, Julie Bouckaert
The most prevalent diseases manifested by Escherichia coli are acute and recurrent bladder infections and chronic inflammatory bowel diseases such as Crohn's disease. E. coli clinical isolates express the FimH adhesin, which consists of a mannose-specific lectin domain connected via a pilin domain to the tip of type 1 pili. Although the isolated FimH lectin domain has affinities in the nanomolar range for all high-mannosidic glycans, differentiation between these glycans is based on their capacity to form predominantly hydrophobic interactions within the tyrosine gate at the entrance to the binding pocket...
January 1, 2017: IUCrJ
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