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Marie Eriksson, Leena Jylli, Li Villard, Anna-Karin Kroksmark, Åsa Bartonek
BACKGROUND: Joint contractures are the main characteristics for children with arthrogryposis multiplex congenita. Orthoses are often used to enable or facilitate walking. OBJECTIVES: To describe health-related quality of life in children with arthrogryposis multiplex congenita and satisfaction with orthoses in those using orthoses. STUDY DESIGN: Cross-sectional study. METHODS: A total of 33 children with arthrogryposis multiplex congenita participated in the study...
May 1, 2018: Prosthetics and Orthotics International
E S Caballero, F G Cuevas, F Ternero, R Astacio, J M Montes, J Cintas
Aluminum matrix composites (AMCs) reinforced by aluminum nitride were prepared by mechanical alloying followed by a simple press and sintering method. Milling began under vacuum and after a period of between 1 and 4 h, NH₃ gas flow (1 cm³/s) was incorporated until the total milling time of 5 h was reached. Results show that in addition to the strain hardening taking place during mechanical alloying, NH₃ plays an additional role in powder hardening. Thereby, the properties of the sintered compacts are strongly influenced by the amount of N incorporated into the powders during milling and the subsequent formation of AlN during the consolidation process...
May 17, 2018: Materials
Wanghua Wu, Hongliang Fan, Xiang Lian, Jianguang Zhou, Tao Zhang
Exponential amplification reaction (EXPAR), as a simple and high sensitive method, holds great promise in nucleic acids detection. One major challenge in EXPAR is the generation of trigger DNA with a definite 3'-end, which now relies on fingerprinting technology. However, the requirement of different endonucleases for varying target sequences and two head-to-head recognition sites in double stranded DNA, as well as the confinement of trigger DNA's 3'-end to be near/within the recognition site, usually subject EXPAR to compromised universality and/or repeated matching of reaction conditions...
August 1, 2018: Talanta
Lihe Zhang, Jianwu Ai, Bowen Jiang, Huchuan Lu, Xiukui Li
In this paper, we propose a bottom-up saliency model based on absorbing Markov chain (AMC). First, a sparsely connected graph is constructed to capture the local context information of each node. All image boundary nodes and other nodes are, respectively, treated as the absorbing nodes and transient nodes in the absorbing Markov chain. Then, the expected number of times from each transient node to all other transient nodes can be used to represent the saliency value of this node. The absorbed time depends on the weights on the path and their spatial coordinates, which are completely encoded in the transition probability matrix...
February 2018: IEEE Transactions on Image Processing: a Publication of the IEEE Signal Processing Society
Sophia G de Vries, Maud M I Bekedam, Benjamin J Visser, Cornelis Stijnis, Pieter P A M van Thiel, Michèle van Vugt, Abraham Goorhuis, Jiri F P Wagenaar, Martin P Grobusch, Marga G A Goris
BACKGROUND: Leptospirosis is a potentially fatal zoonotic disease that is prevalent in travellers. Here, we describe epidemiological and diagnostic characteristics of all returning travellers diagnosed with leptospirosis in the Netherlands between 2009 and 2016. Furthermore, we present a detailed clinical case series of all travellers with leptospirosis who presented at the Academic Medical Center (AMC) in the same period. METHOD: We extracted data from the records of the Dutch Leptospirosis Reference Center (NRL) of all cases of leptospirosis in travellers in the Netherlands from 2009-2016...
May 10, 2018: Travel Medicine and Infectious Disease
Ping Fu, Wei Sun, Juan Lai, Yi-Hong Shen, Ze Zhang
Two isoforms, Bmpop-a and Bmpop-b, were cloned and characterized, which were found to encode prolyl oligopeptidase (Pop) of the domestic silkworm Bombyx mori. The full lengths of Bmpop-a and Bmpop-b were 2497 and 2508 bp, deducing 707 and 740 amino acids, respectively. Both of them, possessing the typical characteristics of the Pop family of serine proteinase, were detected to be expressed among different tissues and development stages at the transcription and translation levels. Soluble recombinant BmPop-a (rBmPop-a) had oligopeptidase activity toward the substrates, Z-Gly-Pro-pNA, Z-Gly-Pro-AMC and angiotensin I...
May 9, 2018: Gene
Tian Zhao, Zhipeng Li, Zhouliang Guo, Ali Wang, Zhenxing Liu, Qing Zhao, Yuyin Li, Edward A McKenzie, Aipo Diao
Legumain (LGMN) is a lysosomal protease that can specifically hydrolyze proteins after carboxyl-terminal asparagine residues. It has been reported that Legumain is highly expressed in many human tumors and promotes the migratory and invasive activity of cancer cells. Due to the limitation of an abundant and affordable source of endogenous active Legumain for further function studies, we produced the recombinant protein in Pichia pastoris. The pPICZα-LGMN expression plasmid was constructed and transformed into Pichia pastoris strain and positive recombinants were identified...
May 7, 2018: Protein Expression and Purification
Yufu Wang, Tianyi Fang, Lining Huang, Hao Wang, Lei Zhang, Zhidong Wang, Yunfu Cui
CD177 is considered to represent neutrophils. We analyzed mRNA expression level of CD177 and clinical follow-up survey of PDAC to estimate overall survival (OS) from Gene Expression Omnibus (GEO) dataset (GSE21501, containing samples from 102 PDAC patients) by R2 platform ( We also analyzed correlated genes of CD177 by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis to predict the potential relationship between neutrophils and prognosis of PDAC. We then performed hematoxylin and eosin (H&E) staining and immunohistochemical staining of surgical specimens to verify infiltration of neutrophils in PDAC tissues...
May 5, 2018: Biochemical and Biophysical Research Communications
Daniela Bencardino, Luca Agostino Vitali, Dezemona Petrelli
The aim of this study was to assess the microbiological quality of ready-to-eat (RTE) iceberg lettuce. Our investigation was based on the consumption tendency of university students considered a target market for this product. A total of 78 RTE samples were collected from chain supermarkets and analysed for the enumeration of aerobic mesophilic count (AMC), Escherichia coli and the detection of Salmonella spp. and Listeria monocytogenes . All samples were negative for the presence of pathogens. The mean value of AMC at the beginning, in the middle and after the expiration date was: 6...
March 31, 2018: Italian Journal of Food Safety
Wanghua Wu, Tao Zhang, Da Han, Hongliang Fan, Guizhi Zhu, Xiong Ding, Cuichen Wu, Mingxu You, Liping Qiu, Juan Li, Liqin Zhang, Xiang Lian, Rong Hu, Ying Mu, Jianguang Zhou, Weihong Tan
We herein describe a simple and versatile approach to use conventional nicking endonuclease (NEase) for programmable sequence-specific cleavage of DNA, termed aligner-mediated cleavage (AMC), and its application to DNA isothermal exponential amplification (AMC-based strand displacement amplification, AMC-SDA). AMC uses a hairpin-shaped DNA aligner (DA) that contains a recognition site in its stem and two side arms complementary to target DNA. Thus, it enables the loading of an NEase on DA's stem, localization to a specific locus through hybridization of the side arms with target DNA, and cleavage thereof...
March 21, 2018: Chemical Science
Hui Wen, Nina Xue, Feng Wu, Yujun He, Gang Zhang, Zebin Hu, Huaqing Cui
Chromenone-derived natural products include chromones (flavone, isoflavone) and coumarins. Chromenone compounds not only exhibit impressive biological activities, but also are an important resource of experimentally used fluorophores, such as, 7-amino-4-methylcoumarin (AMC). Various chromenone compounds have reported to have weak fluorescence, and this has the potential to interfere with the measurements during AMC fluorogenic assays and result in non-robust assay readouts. Several flavones and isoflavones were found as SIRT1 activators, while fluorogenic sirtuin assays utilized AMC labelled peptides as the substrates...
May 2, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Nannan Liu, Huan Yi, Yanyu Lin, Hongli Zheng, Xiangqing Zheng, Danmei Lin, Hong Dai
A dual-responsive sandwich-type immunosensor is described for the detection of interleukin 6 (IL-6) by combining electrochemiluminescent (ECL) and electrochemical (EC) detection based on the use of two kinds of TiO2 mesocrystal nanoarchitectures. A composite was prepared from TiO2 (anatase) mesocages (AMCs) and a carboxy-terminated ionic liquid (CTIL) and then placed on a glassy carbon electrode (GCE). In the next step, the ECL probe Ru(bpy)3 (II) and antibody against IL-6 (Ab1 ) were immobilized on the GCE...
May 2, 2018: Mikrochimica Acta
Marie Shimanuki, Yorihisa Imanishi, Yoichiro Sato, Nana Nakahara, Daisuke Totsuka, Emiri Sato, Sena Iguchi, Yasuo Sato, Keiko Soma, Yasutomo Araki, Seiji Shigetomi, Satoko Yoshida, Kosuke Uno, Yusuke Ogawa, Takehiro Tominaga, Yuichi Ikari, Junko Nagayama, Ayako Endo, Koshiro Miura, Takuya Tomioka, Hiroyuki Ozawa, Kaoru Ogawa
Background: Febrile neutropenia (FN) is the most serious hematologic toxicity of systemic chemotherapy. However, accurate prediction of FN development has been difficult because the risk varies largely depending on the chemotherapy regimen and various individual factors. Methods: We retrospectively analyzed diverse clinical factors including pretreatment hematological parameters to clarify the reliable predictors of FN development during chemotherapy with a docetaxel, cisplatin, and fluorouracil (TPF) regimen in patients with head and neck squamous cell carcinoma...
April 10, 2018: Oncotarget
Laura H Bukkems, Claire Roger, Caspar J Hodiamont, Jean-Yves Lefrant, Nicole P Juffermans, Jason A Roberts, Reinier M van Hest
: Before population pharmacokinetic (PK) models can be clinically applied to aid in dose selection of antibiotics, external validation of the model is warranted. The aim of this study was to assess the predictive performance of a gentamicin population PK model in intensive care unit (ICU) patients in two independent populations of Western critically ill patients. METHODS: Data from the same ICU as used for the earlier model development (Academic Medical Centre, Amsterdam (AMC)) and from the Centre Hospitalier Universitaire de Nîmes (CHU Nîmes) were collected...
April 25, 2018: International Journal of Antimicrobial Agents
Yu Rang Park, Young Jo Yoon, HaYeong Koo, Soyoung Yoo, Chang-Min Choi, Sung-Ho Beck, Tae Won Kim
BACKGROUND: Clinical trials pose potential risks in both communications and management due to the various stakeholders involved when performing clinical trials. The academic medical center has a responsibility and obligation to conduct and manage clinical trials while maintaining a sufficiently high level of quality, therefore it is necessary to build an information technology system to support standardized clinical trial processes and comply with relevant regulations. OBJECTIVE: The objective of the study was to address the challenges identified while performing clinical trials at an academic medical center, Asan Medical Center (AMC) in Korea, by developing and utilizing a clinical trial management system (CTMS) that complies with standardized processes from multiple departments or units, controlled vocabularies, security, and privacy regulations...
April 24, 2018: Journal of Medical Internet Research
Min Li, Jing Zhou, Weifeng Jin, Xiaohong Li, Yuyan Zhang
Background: Hemorrhagic transformation, neurotoxicity, short treatment time windows, and other defects are considered as the major limitations for the thrombolytic therapy. This study is devoted to figure out whether Danhong injection (DHI) combined with tissue-plasminogen activator (t-PA) could extend the treatment time windows and ameliorate brain injury, hemorrhagic complication and BBB disruption after focal embolic stroke. Methods: In vitro , the combined concentrations of DHI and t-PA were added to wells reacted with plasminogen and D-Val-Leu-Lys-AMC...
2018: Frontiers in Pharmacology
Saleem Yousuf Bhat, Arijit Dey, Insaf A Qureshi
Methionine aminopeptidase 2 (MAP2) is a principal regulator of apoptosis for Leishmania donovani and a potential candidate for the design and synthesis of novel antileishmanials. The LdMAP2 gene was cloned in pET28a(+)-SUMO vector, expressed in E. coli and then purified by chromatographic methods. It was found to be a monomer and required divalent metal ion for its activity against synthetic substrates with Co(II), Mg(II), Mn(II) and Ni(II) being the major activators. Moreover, Ca(II) showed the tightest binding with Km value of 124...
April 19, 2018: International Journal of Biological Macromolecules
Preeti Singh, Manju Agrawal, Rajesh Hishikar, Usha Joshi, Basant Maheshwari, Ajay Halwai
BACKGROUND: India is a developing country and adverse drug reactions (ADRs) influence most of the diseases in our population, and monitoring is required due to the paucity of ADRs. The present study was done to analyze the ADRs at the ADR monitoring center (AMC) of tertiary care hospital in Raipur during 1 year. MATERIALS AND METHODS: Study of ADR monitoring of outpatient and inpatient was a prospective and observational study carried out between September 2015 and August 2016...
November 2017: Indian Journal of Pharmacology
Aziza A A Adam, Vincent A van der Mark, Joanne M Donkers, Manon E Wildenberg, Ronald P J Oude Elferink, Robert A F M Chamuleau, Ruurdtje Hoekstra
Practice-changing culturing techniques of hepatocytes are highly required to increase their differentiation. Previously, we found that human liver cell lines HepaRG and C3A acquire higher functionality and increased mitochondrial biogenesis when cultured in the AMC-Bioartificial liver (BAL). Dynamic medium flow (DMF) is one of the major contributors to this stimulatory effect. Recently, we found that DMF-culturing by shaking of HepaRG monolayers resulted in higher mitochondrial biogenesis. Here we further investigated the effect of DMF-culturing on energy metabolism and hepatic functionality of HepaRG and C3A monolayers...
2018: PloS One
Patrick J Silva, Kenneth S Ramos
Innovation ecosystems tied to academic medical centers (AMCs) are inextricably linked to policy, practices, and infrastructure resulting from the passage of the Bayh-Dole Act in 1980. Bayh-Dole smoothed the way to patenting and licensing new drugs, and to some degree, medical devices and diagnostic reagents. Property rights under Bayh-Dole provided a significant incentive for industry investments in clinical trials, clinical validation, and industrial scale-up of products that advanced health care. Bayh-Dole amplified private investment in biotechnology drug development, and from the authors' perspective did not significantly interfere with the ability of AMCs to produce excellent peer-reviewed science...
April 17, 2018: Academic Medicine: Journal of the Association of American Medical Colleges
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