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https://www.readbyqxmd.com/read/28522438/anti-inflammatory-effects-of-oxpapc-involve-endothelial-cell-mediated-generation-of-lxa4
#1
Yunbo Ke, Noureddine Zebda, Olga Oskokova, Taras Afonyushkin, Evgeny Berdyshev, Yufeng Tian, Fanyong Meng, Nicolene Sarich, Valery N Bochkov, Ji Ming Wang, Anna A Birukova, Konstantin G Birukov
Rationale: Oxidation of 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine generates a group of bioactive oxidized phospholipid products (OxPAPC) with a broad range of biological activities. Barrier-enhancing and anti-inflammatory effects of OxPAPC on pulmonary endothelial cells (EC) are critical for prevention of acute lung injury caused by bacterial pathogens or excessive mechanical ventilation. Anti-inflammatory properties of OxPAPC are associated with its antagonistic effects on toll-like receptors and suppression of RhoA GTPase signaling...
May 18, 2017: Circulation Research
https://www.readbyqxmd.com/read/28518085/analysis-of-chromosome-segregation-histone-acetylation-and-spindle-morphology-in-horse-oocytes
#2
Federica Franciosi, Irene Tessaro, Rozenn Dalbies-Tran, Cecile Douet, Fabrice Reigner, Stefan Deleuze, Pascal Papillier, Ileana Miclea, Valentina Lodde, Alberto M Luciano, Ghylene Goudet
The field of assisted reproduction has been developed to treat infertility in women, companion animals, and endangered species. In the horse, assisted reproduction also allows for the production of embryos from high performers without interrupting their sports career and contributes to an increase in the number of foals from mares of high genetic value. The present manuscript describes the procedures used for collecting immature and mature oocytes from horse ovaries using ovum pick-up (OPU). These oocytes were then used to investigate the incidence of aneuploidy by adapting a protocol previously developed in mice...
May 11, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28504425/active-surveillance-to-update-county-scale-distribution-of-four-tick-species-of-medical-and-veterinary-importance-in-oklahoma
#3
Jessica R Mitcham, Anne W Barrett, Jeff M Gruntmeir, Taylor Holland, Jaclyn E Martin, Eileen M Johnson, Susan E Little, Bruce H Noden
The incidence of tick-borne disease continues to increase in humans and companion animals in the United States, yet distribution maps for several tick vectors in Oklahoma, including Dermacentor variabilis, Dermacentor albipictus, Ixodes scapularis, and Amblyomma maculatum, are not available or are outdated. To address this issue, county-scale tick records from peer-reviewed literature and passive collections were reviewed for Oklahoma. Additionally, dry ice traps, tick drags, and harvested deer were utilized to actively collect adult ticks throughout the state...
June 2017: Journal of Vector Ecology: Journal of the Society for Vector Ecology
https://www.readbyqxmd.com/read/28480589/quantification-and-tracking-of-genetically-engineered-dendritic-cells-for-studying-immunotherapy
#4
Amnon Bar-Shir, Lina Alon, Michael J Korrer, Hong Seo Lim, Nirbhay N Yadav, Yoshinori Kato, Arvind P Pathak, Jeff W M Bulte, Assaf A Gilad
PURPOSE: Genetically encoded reporters can assist in visualizing biological processes in live organisms and have been proposed for longitudinal and noninvasive tracking of therapeutic cells in deep tissue. Cells can be labeled in situ or ex vivo and followed in live subjects over time. Nevertheless, a major challenge for reporter systems is to identify the cell population that actually expresses an active reporter. METHODS: We have used a nucleoside analog, pyrrolo-2'-deoxycytidine, as an imaging probe for the putative reporter gene, Drosophila melanogaster 2'-deoxynucleoside kinase...
May 7, 2017: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/28475125/melatonin-promotes-the-in-vitro-development-of-microinjected-pronuclear-mouse-embryos-via-its-anti-oxidative-and-anti-apoptotic-effects
#5
Xiuzhi Tian, Feng Wang, Lu Zhang, Pengyun Ji, Jing Wang, Dongying Lv, Guangdong Li, Menglong Chai, Zhengxing Lian, Guoshi Liu
CRISPR/Cas9 (Clustered regularly interspaced short palindromic repeats) combined with pronuclear microinjection has become the most effective method for producing transgenic animals. However, the relatively low embryo developmental rate limits its application. In the current study, it was observed that 10(-7) M melatonin is considered an optimum concentration and significantly promoted the in vitro development of murine microinjected pronuclear embryos, as indicated by the increased blastocyst rate, hatching blastocyst rate and blastocyst cell number...
May 5, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28472113/modelling-seasonal-habitat-suitability-for-wide-ranging-species-invasive-wild-pigs-in-northern-australia
#6
Jens G Froese, Carl S Smith, Peter A Durr, Clive A McAlpine, Rieks D van Klinken
Invasive wildlife often causes serious damage to the economy and agriculture as well as environmental, human and animal health. Habitat models can fill knowledge gaps about species distributions and assist planning to mitigate impacts. Yet, model accuracy and utility may be compromised by small study areas and limited integration of species ecology or temporal variability. Here we modelled seasonal habitat suitability for wild pigs, a widespread and harmful invader, in northern Australia. We developed a resource-based, spatially-explicit and regional-scale approach using Bayesian networks and spatial pattern suitability analysis...
2017: PloS One
https://www.readbyqxmd.com/read/28465538/how-doctors-diagnose-diseases-and-prescribe-treatments-an-fmri-study-of-diagnostic-salience
#7
Marcio Melo, Gustavo D F Gusso, Marcelo Levites, Edson Amaro, Eduardo Massad, Paulo A Lotufo, Peter Zeidman, Cathy J Price, Karl J Friston
Understanding the brain mechanisms involved in diagnostic reasoning may contribute to the development of methods that reduce errors in medical practice. In this study we identified similar brain systems for diagnosing diseases, prescribing treatments, and naming animals and objects using written information as stimuli. Employing time resolved modeling of blood oxygen level dependent (BOLD) responses enabled time resolved (400 milliseconds epochs) analyses. With this approach it was possible to study neural processes during successive stages of decision making...
May 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28448430/family-dog-assisted-adapted-physical-activity-a-case-study
#8
Amanda Tepfer, Samantha Ross, Megan MacDonald, Monique A R Udell, Craig Ruaux, Wendy Baltzer
Purpose: The aim of this case study was to examine the individual effects of an adapted physical activity, animal-assisted intervention (APA-AAI) with the family dog on motor skills, physical activity, and quality of life of a child with cerebral palsy (CP). Method: This study used an A-B-A single-subject design. The assessment phase (phase A) occurred pre- and post-intervention. This consisted of standardized assessments of motor skills, quality of life questionnaires, physical activity (measured using the GT3X+ accelerometer) and the human-animal bond...
April 27, 2017: Animals: An Open Access Journal From MDPI
https://www.readbyqxmd.com/read/28437455/effect-of-a-probiotic-beverage-consumption-enterococcus-faecium-crl-183-and-bifidobacterium-longum-atcc-15707-in-rats-with-chemically-induced-colitis
#9
Larissa Sbaglia Celiberto, Raquel Bedani, Naiara Naiana Dejani, Alexandra Ivo de Medeiros, José Antonio Sampaio Zuanon, Luis Carlos Spolidorio, Maria Angela Tallarico Adorno, Maria Bernadete Amâncio Varesche, Fábio Carrilho Galvão, Sandro Roberto Valentini, Graciela Font de Valdez, Elizeu Antonio Rossi, Daniela Cardoso Umbelino Cavallini
BACKGROUND: Some probiotic strains have the potential to assist in relieving the symptoms of inflammatory bowel disease. The impact of daily ingestion of a soy-based product fermented by Enterococcus faecium CRL 183 and Lactobacillus helveticus 416 with the addition of Bifidobacterium longum ATCC 15707 on chemically induced colitis has been investigated thereof within a period of 30 days. METHODS: Colitis was induced by dextran sulfate sodium. The animals were randomly assigned into five groups: Group C: negative control; Group CL: positive control; Group CLF: DSS with the fermented product; Group CLP: DSS with the non-fermented product (placebo); Group CLS: DSS with sulfasalazine...
2017: PloS One
https://www.readbyqxmd.com/read/28427645/ultrasound-assisted-extraction-of-achyrocline-satureioides-prevents-contrast-induced-nephropathy-in-mice
#10
Ketheley L Guss, Stefano Pavanni, Bruno Prati, Lucas Dazzi, Jairo P de Oliveira, Breno V Nogueira, Thiago M C Pereira, Marcio Fronza, Denise C Endringer, Rodrigo Scherer
Achyrocline satureioides or Macela, has been largely used in traditional folk medicine in Brazil as an anti-inflammatory agent and to treat various digestive disorders. The aim of the present study was to evaluate the preventive action of the extracts of A. satureioides obtained by maceration and ultrasound-assisted extraction, quercetin and N-acetylcysteine against contrast-induced nephropathy in mice. The antioxidant activity, cytotoxicity and inhibition of nitric oxide (NO) production in macrophages were evaluated...
July 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28425493/characterization-of-different-microbubbles-in-assisting-focused-ultrasound-induced-blood-brain-barrier-opening
#11
Sheng-Kai Wu, Po-Chun Chu, Wen-Yen Chai, Shih-Tsung Kang, Chih-Hung Tsai, Ching-Hsiang Fan, Chih-Kuang Yeh, Hao-Li Liu
Microbubbles (MBs) serve as a critical catalyst to amplify local cavitation in CNS capillary lumen to facilitate focused ultrasound (FUS) to transiently open the blood-brain barrier (BBB). However, limited understanding is available regarding the effect of different microbubbles to induce BBB opening. The aim of this study is to characterize different MBs on their effect in FUS-induced BBB opening. Three MBs, SonoVue, Definity, and USphere, were tested, with 0.4-MHz FUS exposure at 0.62-1.38 of mechanical index (MI) on rats...
April 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28387721/dietary-sources-and-bioactivities-of-melatonin
#12
REVIEW
Xiao Meng, Ya Li, Sha Li, Yue Zhou, Ren-You Gan, Dong-Ping Xu, Hua-Bin Li
Insomnia is a serious worldwide health threat, affecting nearly one third of the general population. Melatonin has been reported to improve sleep efficiency and it was found that eating melatonin-rich foods could assist sleep. During the last decades, melatonin has been widely identified and qualified in various foods from fungi to animals and plants. Eggs and fish are higher melatonin-containing food groups in animal foods, whereas in plant foods, nuts are with the highest content of melatonin. Some kinds of mushrooms, cereals and germinated legumes or seeds are also good dietary sources of melatonin...
April 7, 2017: Nutrients
https://www.readbyqxmd.com/read/28366777/sustained-attention-to-the-owner-is-enhanced-in-dogs-trained-for-animal-assisted-interventions
#13
Paolo Mongillo, Elisa Pitteri, Lieta Marinelli
Adaptation in human societies requires dogs to pay attention to socially relevant human beings, in contexts that may greatly vary in social complexity. In turn, such selective attention may depend on the dog's training and involvement in specific activities. Therefore, we recruited untrained pet dogs (N=32), dogs trained for agility (N=32) and for animal assisted interventions (N=32) to investigate differences in attention to the owner in relation to the dogs' training/working experience. Average gaze length and frequency of gaze shifting towards the owner were measured in a 'baseline attention test', where dogs were exposed to the owner walking in and out of the experimental room and in a 'selective attention test', where the owner's movements were mirrored by an unfamiliar figurant...
March 31, 2017: Behavioural Processes
https://www.readbyqxmd.com/read/28363483/restoration-of-reaching-and-grasping-movements-through-brain-controlled-muscle-stimulation-in-a-person-with-tetraplegia-a-proof-of-concept-demonstration
#14
A Bolu Ajiboye, Francis R Willett, Daniel R Young, William D Memberg, Brian A Murphy, Jonathan P Miller, Benjamin L Walter, Jennifer A Sweet, Harry A Hoyen, Michael W Keith, P Hunter Peckham, John D Simeral, John P Donoghue, Leigh R Hochberg, Robert F Kirsch
BACKGROUND: People with chronic tetraplegia, due to high-cervical spinal cord injury, can regain limb movements through coordinated electrical stimulation of peripheral muscles and nerves, known as functional electrical stimulation (FES). Users typically command FES systems through other preserved, but unrelated and limited in number, volitional movements (eg, facial muscle activity, head movements, shoulder shrugs). We report the findings of an individual with traumatic high-cervical spinal cord injury who coordinated reaching and grasping movements using his own paralysed arm and hand, reanimated through implanted FES, and commanded using his own cortical signals through an intracortical brain-computer interface (iBCI)...
May 6, 2017: Lancet
https://www.readbyqxmd.com/read/28357477/wnt5a-is-elevated-in-heart-failure-and-affects-cardiac-fibroblast-function
#15
Aurelija Abraityte, Leif E Vinge, Erik T Askevold, Tove Lekva, Annika E Michelsen, Trine Ranheim, Katrine Alfsnes, Arnt Fiane, Svend Aakhus, Ida G Lunde, Christen P Dahl, Pål Aukrust, Geir Christensen, Lars Gullestad, Arne Yndestad, Thor Ueland
Wnt signaling is dysregulated in heart failure (HF) and may promote cardiac hypertrophy, fibrosis, and inflammation. Blocking the Wnt ligand Wnt5a prevents HF in animal models. However, the role of Wnt5a in human HF and its functions in cardiac cells remain unclear. Here, we investigated Wnt5a regulation in HF patients and its effects on primary mouse and human cardiac fibroblasts. Serum Wnt5a was elevated in HF patients and associated with hemodynamic, neurohormonal, and clinical measures of disease severity...
March 30, 2017: Journal of Molecular Medicine: Official Organ of the "Gesellschaft Deutscher Naturforscher und Ärzte"
https://www.readbyqxmd.com/read/28348566/lifetime-modulation-of-the-pain-system-via-neuroimmune-and-neuroendocrine-interactions
#16
REVIEW
Ihssane Zouikr, Bianka Karshikoff
Chronic pain is a debilitating condition that still is challenging both clinicians and researchers. Despite intense research, it is still not clear why some individuals develop chronic pain while others do not or how to heal this disease. In this review, we argue for a multisystem approach to understand chronic pain. Pain is not only to be viewed simply as a result of aberrant neuronal activity but also as a result of adverse early-life experiences that impact an individual's endocrine, immune, and nervous systems and changes which in turn program the pain system...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28344974/assessing-preferences-for-animals-in-children-with-autism-a-new-use-for-video-based-preference-assessment
#17
REVIEW
Noémie A Guérin, Kerri E Rodriguez, Matthew T Brodhead, Marguerite E O'Haire
The inclusion of animals into interventions for children with autism spectrum disorder (ASD) is a growing practice known as animal-assisted intervention (AAI). The choice of the animal to include in an intervention is often solely up to the interventionist and depends on their experience, subjective judgment, and ease of access to different animals. For individuals with ASD who are non-verbal and unable to indicate preferred stimuli or activities, incorporating preference into interventions has been linked to increases in positive behaviors and enhanced quality of life...
2017: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/28344031/culture-bound-syndromes-of-a-brazilian-amazon-riverine-population-tentative-correspondence-between-traditional-and-conventional-medicine-terms-and-possible-ethnopharmacological-implications
#18
E Pagani, J de F L Santos, E Rodrigues
ETHNOPHARMACOLOGICAL RELEVANCE: It is not always possible to correlate the "emic" terms to the "etic" ones during ethnopharmacological surveys, especially regarding those related to Culture-Bound Syndromes (CBS). Nevertheless, it is the role of ethnopharmacology to address these correlations, since they are the basis for the understanding of potential bioactives. AIM AND OBJECTIVES: This study reports the clinical manifestations and therapeutic resources used for the treatment of CBS among some riverine inhabitants of Brazilian Amazonia...
May 5, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28333263/loss-of-luteotropic-prostaglandin-e-plays-an-important-role-in-the-regulation-of-luteolysis-in-women
#19
Junko Nio-Kobayashi, Masataka Kudo, Noriaki Sakuragi, Toshihiko Iwanaga, W Colin Duncan
STUDY QUESTION: Do intraluteal prostaglandins (PG) contribute to luteal regulation in women? SUMMARY ANSWER: Prostaglandin E (PGE), which is produced in human granulosa-lutein cells stimulated with luteotropic hCG, exerts similar luteotropic effects to hCG, and the expression of PG synthetic and metabolic enzymes in the human CL is driven toward less PGE but more prostaglandin F (PGF) during luteolysis. WHAT IS KNOWN ALREADY: Uterine PGF is a major luteolysin in many non-primate species but not in women...
May 1, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28324925/non-invasive-imaging-of-ventricular-activation-during-pacing-and-arrhythmia-methods-and-validation
#20
Long Yu, Steven Pogwizd, Bin He
Cardiovascular disease continued to be a leading killer world widely. Each year, about 400,000 cases of sudden cardiac arrest are reported in the U.S. alone. Clinically, radio-frequency ablative procedure has become widely applied in the treatment of ventricular arrhythmia. Non-invasive approaches have been demonstrated to be able to provide important information on the arrhythmogenesis and potentially assist in the clinical practice. In this work, we develop and validate a novel temporal sparse based imaging method, Cardiac Electrical Sparse Imaging (CESI)...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
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