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Leukocyte rich platelet rich plasma

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https://www.readbyqxmd.com/read/28210651/analysis-of-platelet-rich-plasma-extraction-variations-in-platelet-and-blood-components-between-4-common-commercial-kits
#1
Jane Fitzpatrick, Max K Bulsara, Paul Robert McCrory, Martin D Richardson, Ming Hao Zheng
BACKGROUND: Platelet-rich plasma (PRP) has been extensively used as a treatment in tissue healing in tendinopathy, muscle injury, and osteoarthritis. However, there is variation in methods of extraction, and this produces different types of PRP. PURPOSE: To determine the composition of PRP obtained from 4 commercial separation kits, which would allow assessment of current classification systems used in cross-study comparisons. STUDY DESIGN: Controlled laboratory study...
January 2017: Orthopaedic Journal of Sports Medicine
https://www.readbyqxmd.com/read/28146403/hyaluronic-acid-versus-platelet-rich-plasma
#2
Brian J Cole, Vasili Karas, Kristen Hussey, Kyle Pilz, Lisa A Fortier
BACKGROUND: The use of platelet-rich plasma (PRP) for the treatment of osteoarthritis (OA) has demonstrated mixed clinical outcomes in randomized controlled trials when compared with hyaluronic acid (HA), an accepted nonsurgical treatment for symptomatic OA. Biological analysis of PRP has demonstrated an anti-inflammatory effect on the intra-articular environment. PURPOSE: To compare the clinical and biological effects of an intra-articular injection of PRP with those of an intra-articular injection of HA in patients with mild to moderate knee OA...
February 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/28056978/short-term-storage-stability-at-room-temperature-of-two-different-platelet-rich-plasma-preparations-from-equine-donors-and-potential-impact-on-growth-factor-concentrations
#3
Gregor Hauschild, Florian Geburek, Georg Gosheger, Maria Eveslage, Daniela Serrano, Arne Streitbürger, Sara Johannlükens, Dirk Menzel, Reinhard Mischke
BACKGROUND: The increasing interest in platelet-rich plasma (PRP) based therapies is as yet accompanied by inconsistent information regarding nearly all aspects of handling and application. Among these storage stability of processed platelet-rich products may be the basis for a more flexible application mode. The objective of this study was (1) to estimate the storage stability of growth factors platelet derived growth factor BB (PDGF-BB) and transforming growth factor ß1 (TGF-ß1) in both, a single-step softspin centrifugation-based pure-PRP (P-PRP, ACP®), and a gravity filtration system-based leukocyte-rich-PRP (L-PRP, E-PET), over a six hours time span after preparation at room temperature and (2) to identify possible factors influencing these growth factor concentrations in an equine model...
January 5, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28027451/the-use-of-platelet-rich-and-platelet-poor-plasma-to-enhance-differentiation-of-skeletal-myoblasts
#4
Olga Miroshnychenko, Wen-Teh Chang, Jason L Dragoo
BACKGROUND: Platelet-rich plasma (PRP) has been used to augment tissue repair and regeneration after musculoskeletal injury. However, there is increasing clinical evidence that PRP does not show a consistent clinical effect. Purpose/Hypothesis: This study aimed to compare the effects of the following non-neutrophil-containing (leukocyte-poor) plasma fractions on human skeletal muscle myoblast (HSMM) differentiation: (1) PRP, (2) modified PRP (Mod-PRP), in which transforming growth factor β1 (TGF-β1) and myostatin (MSTN) were depleted, and (3) platelet-poor plasma (PPP)...
December 1, 2016: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/28018086/leukocyte-poor-platelet-rich-plasma-to-treat-degenerative-meniscal-tear-a-case-report
#5
J P Betancourt, W D Murrell
A traumatic and/or degenerative meniscus lesion is thought to be a clinical manifestation of early-onset osteoarthritis (OA), which is a chronic progressive condition that can cause substantial pain and disability. Platelet-rich plasma (PRP) is an emerging treatment option that has been reported to improve healing. Here, we present a case of a 29-year-old woman, with left anterior and medial knee pain, without history of trauma or injury. The patient was managed with leukocyte-poor PRP injections derived from her peripheral blood with high concentrations of platelets, platelet-derived growth factors, and bioactive proteins, with a total follow-up of 30 months...
October 2016: Journal of Clinical Orthopaedics and Trauma
https://www.readbyqxmd.com/read/27900294/efficacy-of-autologous-leukocyte-reduced-platelet-rich-plasma-therapy-for-patellar-tendinopathy-in-a-rat-treadmill-model
#6
Mamoru Yoshida, Hiroki Funasaki, Keishi Marumo
BACKGROUND: An autologous platelet-rich plasma (PRP) therapy has currently been applied for the tendinopathy; however, its efficacy and an optimal platelets concentration in PRP were uncertain. We analyzed them in an animal model prepared using a repetitive running exercise. METHODS: We made the tendinopathy rat model of patellar tendon using a rodent treadmill machine. Rats with tendinopathy were injected with leukocyte-reduced PRP at the platelets concentration of 1...
April 2016: Muscles, Ligaments and Tendons Journal
https://www.readbyqxmd.com/read/27849108/influence-of-calcium-salts-and-bovine-thrombin-on-growth-factor-release-from-equine-platelet-rich-gel-supernatants
#7
Carlos E Giraldo, María E Álvarez, Jorge U Carmona
OBJECTIVE: To compare five activation methods in equine platelet-rich plasma (PRP) by determination of platelet-derived growth factor BB (PDGF-BB) and transforming growth factor beta 1 (TGF-β1) concentrations in platelet-rich gel (PRG) supernatants. METHODS: Platelet-rich plasma from 20 horses was activated by calcium chloride (CC), calcium gluconate (CG), bovine thrombin (BT), and their combinations, BTCC and BTCG. Both growth factor concentrations in PRG supernatants were measured by ELISA and compared with plasma and platelet lysates (PL) over time...
November 16, 2016: Veterinary and Comparative Orthopaedics and Traumatology: V.C.O.T
https://www.readbyqxmd.com/read/27775049/a-high-throughput-microfluidic-approach-for-1000-fold-leukocyte-reduction-of-platelet-rich-plasma
#8
Hui Xia, Briony C Strachan, Sean C Gifford, Sergey S Shevkoplyas
Leukocyte reduction of donated blood products substantially reduces the risk of a number of transfusion-related complications. Current 'leukoreduction' filters operate by trapping leukocytes within specialized filtration material, while allowing desired blood components to pass through. However, the continuous release of inflammatory cytokines from the retained leukocytes, as well as the potential for platelet activation and clogging, are significant drawbacks of conventional 'dead end' filtration. To address these limitations, here we demonstrate our newly-developed 'controlled incremental filtration' (CIF) approach to perform high-throughput microfluidic removal of leukocytes from platelet-rich plasma (PRP) in a continuous flow regime...
October 24, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27759829/analysis-of-cytokine-profile-and-growth-factors-in-platelet-rich-plasma-obtained-by-open-systems-and-commercial-columns
#9
Alberto de Castro Pochini, Eliane Antonioli, Daniella Zanetti Bucci, Luiz Roberto Sardinha, Carlos Vicente Andreoli, Mario Ferretti, Benno Ejnisman, Anna Carla Goldberg, Moisés Cohen
Objective: To evaluate growth factors and cytokines in samples of platelet-rich plasma obtained by three different centrifugation methods. Methods: Peripheral blood of six individuals with no hematological diseases, aged 18 to 68 years, was drawn to obtain platelet-rich plasma, using the open method and commercial columns by Medtronic and Biomet. The products obtained with the different types of centrifugation were submitted to laboratory analysis, including pro-inflammatory cytokines and chemokines by flow cytometry assays, the concentration of fibroblast growth factors-2 (FGF-2) and transforming growth factor-beta1 (TGF-β1)...
July 2016: Einstein
https://www.readbyqxmd.com/read/27706013/the-early-histological-effects-of-intravesical-instillation-of-platelet-rich-plasma-in-cystitis-models
#10
M İrfan Dönmez, Kubilay İnci, Naciye Dilara Zeybek, H Serkan Doğan, Ali Ergen
PURPOSE: To evaluate the early histological effects of the intravesical instillation of platelet-rich plasma (PRP) in rabbit models of interstitial and hemorrhagic cystitis. METHODS: Thirty-six rabbits were classified into 6 groups: saline (S), S+PRP, hydrochloric acid (HCl), HCl+PRP, cyclophosphamide (CyP), and CyP+PRP. At 48 hours after induction, PRP was prepared and intravesically administered to the S+PRP, HCl+PRP, and CyP+PRP groups. Bladder sections were stained with toluidine blue for mast cell counting and with hematoxylin and eosin for histopathology and mitotic index determination...
September 2016: International Neurourology Journal
https://www.readbyqxmd.com/read/27677100/steroid-vs-platelet-rich-plasma-in-ultrasound-guided-sacroiliac-joint-injection-for-chronic-low-back-pain
#11
Varun Singla, Yatindra K Batra, Neerja Bharti, Vijay G Goni, Neelam Marwaha
BACKGROUND: Despite widespread use of steroids to treat sacroiliac joint (SIJ) pain, their duration of pain reduction is short. Platelet-rich plasma (PRP) can potentially enhance tissue healing and may have a longer-lasting effect on pain. OBJECTIVES: To assess the efficacy and safety of PRP compared with methylprednisolone in ultrasound-guided SIJ injection for low back pain. STUDY DESIGN: Prospective randomized open blinded end point (PROBE) study...
September 27, 2016: Pain Practice: the Official Journal of World Institute of Pain
https://www.readbyqxmd.com/read/27589083/an-efficient-method-for-isolation-of-representative-and-contamination-free-population-of-blood-platelets-for-proteomic-studies
#12
Aneta Wrzyszcz, Joanna Urbaniak, Agnieszka Sapa, Mieczysław Woźniak
To date, there has been no ideal method for blood platelet isolation which allows one to obtain a preparation devoid of contaminations, reflecting the activation status and morphological features of circulating platelets. To address these requirements, we have developed a method which combines the continuous density gradient centrifugation with washing from PGI2-supplemented platelet-rich plasma (PRP). We have assessed the degree of erythrocyte and leukocyte contamination, recovery of platelets, morphological features, activation status, and reactivity of isolated platelets...
January 2017: Platelets
https://www.readbyqxmd.com/read/27506585/leukocyte-poor-platelet-rich-plasma-is-more-effective-than-the-conventional-therapy-with-acetaminophen-for-the-treatment-of-early-knee-osteoarthritis
#13
RANDOMIZED CONTROLLED TRIAL
Mario Simental-Mendía, José F Vílchez-Cavazos, Víctor M Peña-Martínez, Salvador Said-Fernández, Jorge Lara-Arias, Herminia Guadalupe Martínez-Rodríguez
INTRODUCTION: Knee osteoarthritis (OA) is a degenerative and progressive articular cartilage disease. Infiltration of autologous platelet-rich plasma (PRP) has been proposed as a therapeutic alternative due to the content of biologically active cytokines in PRP. We aimed to compare the clinical response of acetaminophen and intra-articular leukocyte-poor PRP (LP-PRP) in early knee OA. MATERIALS AND METHODS: A total of 65 patients with clinically and radiographically documented knee OA (grade 1-2) were analyzed...
December 2016: Archives of Orthopaedic and Trauma Surgery
https://www.readbyqxmd.com/read/27503185/leukocyte-concentration-and-composition-in-platelet-rich-plasma-prp-influences-the-growth-factor-and-protease-concentrations
#14
Yohei Kobayashi, Yoshitomo Saita, Hirofumi Nishio, Hiroshi Ikeda, Yuji Takazawa, Masashi Nagao, Tomoiku Takaku, Norio Komatsu, Kazuo Kaneko
BACKGROUND: Platelet-rich plasma (PRP) therapy has become an increasingly popular treatment for orthopaedics and sports-related injuries, and various clinically available PRP preparation methods exist. However, the differences in PRP quality among numerous preparation methods remain unclear. Specifically, the benefit of including leukocytes in the PRP product remains controversial, and few studies have been conducted to evaluate the effects of the interaction between platelets and leukocytes on the growth factor concentrations...
September 2016: Journal of Orthopaedic Science: Official Journal of the Japanese Orthopaedic Association
https://www.readbyqxmd.com/read/27478842/leukocyte-reduced-platelet-rich-plasma-treatment-of-basal-thumb-arthritis-a-pilot-study
#15
Markus Loibl, Siegmund Lang, Lena-Marie Dendl, Michael Nerlich, Peter Angele, Sebastian Gehmert, Michaela Huber
A positive effect of intra-articular platelet-rich plasma (PRP) injection has been discussed for osteoarthritic joint conditions in the last years. The purpose of this study was to evaluate PRP injection into the trapeziometacarpal (TMC) joint. We report about ten patients with TMC joint osteoarthritis (OA) that were treated with 2 intra-articular PRP injections 4 weeks apart. PRP was produced using the Double Syringe System (Arthrex Inc., Naples, Florida, USA). A total volume of 1.47 ± 0.25 mL PRP was injected at the first injection and 1...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27397996/in-vitro-study-of-the-role-of-thrombin-in-platelet-rich-plasma-prp-preparation-utility-for-gel-formation-and-impact-in-growth-factors-release
#16
Stephany Cares Huber, José Luiz Rosenberis Cunha Júnior, Silmara Montalvão, Letícia Queiroz da Silva, Aline Urban Paffaro, Francesca Aparecida Ramos da Silva, Bruno Lima Rodrigues, José Fabio Santos Duarte Lana, Joyce Maria Annichino-Bizzacchi
INTRODUCTION: The use of PRP has been studied for different fields, with promising results in regenerative medicine. Until now, there is no study in the literature evaluating thrombin levels in serum, used as autologous thrombin preparation. Therefore, in the present study we evaluated the role played by different thrombin concentrations in PRP and the impact in the release of growth factors. Also, different activators for PRP gel formation were evaluated. METHODS: Thrombin levels were measured in different autologous preparations: serum, L-PRP (PRP rich in leukocytes) and T-PRP (thrombin produced through PRP added calcium gluconate)...
2016: Journal of Stem Cells & Regenerative Medicine
https://www.readbyqxmd.com/read/27377748/the-influence-of-environmental-variables-on-platelet-concentration-in-horse-platelet-rich-plasma
#17
Riccardo Rinnovati, Noemi Romagnoli, Fabio Gentilini, Carlotta Lambertini, Alessandro Spadari
Platelet-rich plasma (PRP) commonly refers to blood products which contain a higher platelet (PLT) concentration as compared to normal plasma. Autologous PRP has been shown to be safe and effective in promoting the natural processes of soft tissue healing or reconstruction in humans and horses. Variability in PLT concentration has been observed in practice between PRP preparations from different patients or from the same individual under different conditions. A change in PLT concentration could modify PRP efficacy in routine applications...
July 4, 2016: Acta Veterinaria Scandinavica
https://www.readbyqxmd.com/read/27369779/in-vitro-effects-of-platelet-rich-gel-supernatants-on-histology-and-chondrocyte-apoptosis-scores-hyaluronan-release-and-gene-expression-of-equine-cartilage-explants-challenged-with-lipopolysaccharide
#18
Jorge U Carmona, Diana L Ríos, Catalina López, María E Álvarez, Jorge E Pérez, Mabel E Bohórquez
BACKGROUND: Platelet-rich plasma (PRP) preparations are a common treatment in equine osteoarthritis (OA). However, there are controversies regarding the ideal concentration of platelets and leukocytes in these biological substances necessary to induce an adequate anti-inflammatory and anabolic response in articular cartilage. The aims were to study the influence of leukocyte- and platelet-rich gel (L-PRG) and pure platelet-rich gel (P-PRG) supernatants on the histological changes of cartilage, the degree of chondrocyte apoptosis, the production of hyaluronan (HA) and the gene expression of nuclear factor kappa beta (NFkβ), matrix metalloproteinase 13 (MMP-13), a disintegrin and metalloproteinase with thrombospondin motifs 4 (ADAMTS-4), collagen type I alpha 1 (COL1A1), collagen type II alpha 1 (COL2A1) and cartilage oligomeric matrix protein (COMP) in normal cartilage explants (CEs) challenged with lipopolysaccharide (LPS)...
July 1, 2016: BMC Veterinary Research
https://www.readbyqxmd.com/read/27350440/regulation-of-the-lysophosphatidylserine-and-sphingosine-1-phosphate-levels-in-autologous-whole-blood-by-the-pre-storage-leukocyte-reduction
#19
Y Nagura, N H Tsuno, K Kano, A Inoue, J Aoki, Y Hirowatari, M Kaneko, M Kurano, M Matsuhashi, R Ohkawa, M Tozuka, Y Yatomi, H Okazaki
BACKGROUND AND OBJECTIVES: The effect of leukoreduction and storage periods on the accumulation of bioactive lysophospholipids and substances in human autologous blood (AB units) has not been fully investigated. MATERIALS AND METHODS: The accumulation of bioactive lysophospholipids such as sphingosine 1-phosphate (S1P) and lysophosphatidylserine (LysoPS) in AB units during the storage was investigated. The time-dependent changes and the effect of the filtration in pre-storage leuckoreduction (LR) and unmodified samples derived from 46 AB units were analysed...
June 28, 2016: Transfusion Medicine
https://www.readbyqxmd.com/read/27318752/medication-related-osteonecrosis-of-the-jaw-is-autologous-platelet-concentrate-application-effective-for-prevention-and-treatment-a%C3%A2-systematic-review
#20
Pia Lopez-Jornet, Arturo Sanchez Perez, Rui Amaral Mendes, Aurelio Tobias
PURPOSE: To date, no ideal strategy has been established for treating or preventing medication-related osteonecrosis of the jaw (MRONJ). The aim of the present work was to perform a systematic literature review to determine the efficacy of autologous platelet concentrate (APC) application, for prevention or treatment of MRONJ, together with surgical debridement. MATERIAL AND METHODS: An electronic search was performed using MEDLINE (PubMed), EMBASE, and Cochrane databases until January 2015 using the following search terms: osteonecrosis, bisphosphonates, antiresorptive, antiangiogenic therapy, BRONJ, platelet concentrate, PRP, PRF, and PRGF...
August 2016: Journal of Cranio-maxillo-facial Surgery
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