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Leukocyte rich platelet rich plasma

Richard T Amison, Shirin Jamshidi, Khondaker M Rahman, Clive P Page, Simon C Pitchford
Platelet P2Y1 receptor signaling via RhoGTPases is necessary for platelet-dependent leukocyte recruitment, where no platelet aggregation is observed. We investigated signaling cascades involved in distinct P2Y1 -dependent platelet activities in vitro, using specific inhibitors for phospholipase C (PLC) (U73122, to inhibit the canonical pathway), and RhoGTPases: Rac1 (NSC23766) and RhoA (ROCK inhibitor GSK429286). Human platelet rich plasma (for platelet aggregation) or isolated washed platelets (for chemotaxis assays) was treated with U73122, GSK429286 or NSC23766 prior to stimulation with adenosine diphosphate (ADP) or the P2Y1 specific agonist MRS2365...
March 10, 2018: European Journal of Pharmacology
Michael D Charles, David R Christian, Brian J Cole
PURPOSE OF REVIEW: The purpose of this review was to establish the foundation of the major biologic adjuvants to rotator cuff repairs and review recent scientific findings. RECENT FINDINGS: Platelet-rich plasma (PRP) overall has no significant impact on functional outcomes and repair integrity, but may be more advantageous in small to medium tears. Further studies should focus on leukocyte-rich versus poor preparations and the use of PRP in patients that are high risk for repair failure...
February 7, 2018: Current Reviews in Musculoskeletal Medicine
Bart W Oudelaar, Joost C Peerbooms, Rianne Huis In 't Veld, Anne J H Vochteloo
BACKGROUND: Platelet-rich plasma (PRP) has proven to be a very safe therapeutic option in the treatment of tendon, muscle, bone, and cartilage injuries. Currently, several commercial separation systems are available for the preparation of PRP. The concentrations of blood components in PRP among these separation systems vary substantially. PURPOSE: To systematically review and evaluate the differences between the concentrations of blood components in PRP produced by various PRP separation systems...
January 1, 2018: American Journal of Sports Medicine
Agata Cieślik-Bielecka, Marcin Pierchała, Aleksandra Królikowska, Paweł Reichert
AIM OF THE STUDY: The aim of the study was to evaluate the effect of leukocyte- and platelet-rich plasma (L-PRP) treatment on a noncomplicated would healing after surgical skin incision in an experimental animal model. MATERIALS AND METHODS: The blood from 64 male Wistar rats was used to prepare L-PRP before a transverse dorsal incision was made and consecutively closed. At the same time after the surgery was completed, autologous L-PRP in the rats from the L-PRP group (n = 32) and NaCl in the control group (n = 32) were injected subcutaneously into the wound...
January 9, 2018: Connective Tissue Research
Mario Simental-Mendía, Félix Vilchez-Cavazos, Rubén García-Garza, Jorge Lara-Arias, Roberto Montes-de-Oca-Luna, Salvador Said-Fernández, Herminia G Martínez-Rodríguez
OBJECTIVE: To determine the effects of autologous leukocyte-poor platelet-rich plasma (LP-PRP) on the expression of markers involved in cartilage-extracellular matrix production and inflammation in cartilage explants bearing osteoarthritis. MATERIALS AND METHODS: Cartilage explants and LP-PRP were obtained from 10 patients who underwent total knee arthroplasty. The explants were cultured in spinner flasks for 28 days in the presence of interleukin (IL)-1β and/or LP-PRP...
January 9, 2018: Histology and Histopathology
Grace Xiong, Nithya Lingampalli, Jayme C B Koltsov, Lawrence L Leung, Nidhi Bhutani, William H Robinson, Constance R Chu
BACKGROUND: Autologous platelet-rich plasma (PRP) is widely used for a variety of clinical applications. However, clinical outcome studies have not consistently shown positive effects. The composition of PRP differs based on many factors. An improved understanding of factors influencing the composition of PRP is important for the optimization of PRP use. HYPOTHESIS: Age and sex influence the PRP composition in healthy patients. STUDY DESIGN: Controlled laboratory study...
February 2018: American Journal of Sports Medicine
Po-Ju Chia-Lai, Anna Orlowska, Sarah Al-Maawi, Andre Dias, Yunxin Zhang, Xuejiu Wang, Niklas Zender, Robert Sader, C James Kirkpatrick, Shahram Ghanaati
OBJECTIVES: This study examines the permeability and barrier capacity of a sugar cross-linked resorbable collagen membrane ex vivo and in vivo. MATERIALS AND METHODS: In an ex vivo study, injectable platelet-rich fibrin (i-PRF), a peripheral blood-derived human leukocyte-and-platelet-rich plasma was used to analyze membrane permeability. in vivo subcutaneous implantation in Wistar rats (n = 4 per time point and group) was used to investigate the barrier capacity of the membrane...
December 6, 2017: Clinical Oral Investigations
Hoi Ting Shiu, Ping Chung Leung, Chun Hay Ko
Bone healing is a complex repair process that commences with the formation of a blood clot at the injured bone, termed hematoma. It has evidenced that a lack of a stable hematoma causes delayed bone healing or non-union. The hematoma at the injured bone constitutes the early healing microenvironment. It appears to dictate healing pathways that ends in a regenerative bone. However, the hematoma is often clinically removed from the damaged site. Conversely, blood-derived products have been used in bone tissue engineering for treating critical sized defects, including fibrin gels and platelet-rich plasma...
December 5, 2017: Journal of Tissue Engineering and Regenerative Medicine
Mamoru Yoshida, Keishi Marumo
Some patients complain of chronic persistent medial knee pain after isolated low-grade injuries of medial collateral ligaments (MCL). These injuries often respond to various conservative treatments but insufficient healing of ligaments is believed to be responsible for symptoms in this group of patients. We report on successful treatment of chronically symptomatic MCL injuries in 3 patients using autologous leukocyte-reduced platelet-rich plasma (PRP) injections. There were 3 men with a mean age of 39.3 years and a mean disease duration of 10 months...
November 29, 2017: Clinical Journal of Sport Medicine: Official Journal of the Canadian Academy of Sport Medicine
J Banks Deal, Ed Smith, Wendell Heard, Michael J O'Brien, Felix H Savoie
Background: Jobe revolutionized the treatment of medial ulnar collateral ligament (MUCL) tears with his reconstruction technique. However, not all MUCL injuries require operative management; Rettig showed that 42% of MUCL injuries respond to conservative management. This was improved by Podesta, who showed that augmentation of nonoperative management with platelet-rich plasma (PRP) and magnetic resonance imaging (MRI) for detecting partial MUCL tears resulted in significantly higher success rates...
November 2017: Orthopaedic Journal of Sports Medicine
Sophie Orliac, Jean-Michel Serfaty, Anne Perozziello, Olivier Zurlinden, Liliane Louedec, Benjamin Dallaudière
PURPOSE: To assess the potential of platelet-rich plasma (PRP) subcutaneous injection of to treat alopecia and to evaluate local toxicity. MATERIALS-METHODS: Twelve Hairless rats were used. At D0, we performed systematic clinical examination and divided the rat back into four quadrants (Q). We initiated subcutaneous injection using either PRP in PRPQ+, platelet-poor plasma (PPP) in PPPQ+, physiological serum (PS) in PSQ+, or no treatment (Q4). At D7, D14, D21, and D28 but also second month (M2), M3, M4, M5, rats had exactly the same injection procedure...
November 11, 2017: Journal of Cosmetic Dermatology
Duygu Yaşar Şirin, İbrahim Yılmaz, Mehmet İsyar, Kadir Öznam, Mahir Mahiroğulları
OBJECTIVES: This study aims to investigate the possible effects of leukocyte concentration in the content of platelet-rich plasma (PRP) and the administration of PRP using a drug delivery system on chondrocyte proliferation in vitro conditions. PATIENTS AND METHODS: Blood from nine male patients (mean age 65 years; range 49 to 81 years) with advanced stage osteoarthritis who had not responded to medical or conservative treatments and underwent total knee arthroplasty was used to prepare two formulations: PRP with low concentration leukocytes (2000-4000 leukocytes/µL) was designated as pure PRP (P-PRP), whereas PRP with high concentration leukocytes (9000-11000 leukocytes/µL) as leukocyte-rich PRP (L-PRP)...
December 2017: Eklem Hastalıkları Ve Cerrahisi, Joint Diseases & related Surgery
Molly C Meadows, David M Levy, Christopher M Ferry, Thomas R Gardner, Takeshi Teratani, Christopher S Ahmad
We conducted a study to determine if platelet-rich plasma (PRP) enhances the strength of rotator cuff repair (RCR) and if concomitant use of nonsteroidal anti-inflammatory drugs (NSAIDs) affects PRP efficacy. We also wanted to determine the optimal centrifugation protocol for making PRP from rats. This study used 48 rats, 14 in a centrifugation protocol and 34 in an operative protocol. Six syngeneic rats from the operative group were used as PRP blood donors; the other 28 operative rats underwent bilateral RCRs...
September 2017: American Journal of Orthopedics
Bruna Alice Gomes de Melo, Andréa Arruda Martins Shimojo, Amanda Gomes Marcelino Perez, José Fabio Santos Duarte Lana, Maria Helena Andrade Santana
Platelet-rich plasma (PRP) is an autologous product prepared from whole blood (WB) that is widely used in regenerative medicine. In clinical practice, discontinuous centrifugation is used for both hand- and machine-prepared PRP. However, separation of WB fractions via centrifugation is a complex process, and the lack of clear mechanisms limits the understanding and evaluation of PRP preparation methods This paper focuses on the distribution, recovery and concentration factor of platelets and leukocytes in L-PRP (leukocyte and platelet-rich plasma) to define a concentration pattern for these blood components due to centrifugation conditions...
January 1, 2018: Colloids and Surfaces. B, Biointerfaces
Isabel Andia, Michele Abate
Current research on common musculoskeletal problems, including osteoarticular conditions, tendinopathies, and muscle injuries, focuses on regenerative translational medicine. Platelet-rich plasma therapies have emerged as a potential approach to enhance tissue repair and regeneration. Platelet-rich plasma application aims to provide supraphysiological concentrations of platelets and optionally leukocytes at injured/pathological tissues mimicking the initial stages of healing. However, the efficacy of platelet-rich plasma is controversial in chronic diseases because patients' outcomes show partial improvements...
October 18, 2017: Frontiers of Medicine
Wenjing Yin, Haitao Xu, Jiagen Sheng, Zhenzhong Zhu, Dongxu Jin, Peichun Hsu, Xuetao Xie, Changqing Zhang
While it has been proved that centrifugal conditions for pure platelet-rich plasma (P-PRP) preparation influence the cellular composition of P-PRP obtained, the optimal centrifugal conditions to prepare P-PRP have not yet been identified. In the present study, platelet-containing plasma (PCP) was prepared with the first-spin of different double-spin methods and P-PRP was prepared with different double-spin methods. Whole-blood analysis was performed to evaluate the cellular composition of PCP and P-PRP. The basal and ADP-induced CD62P expression rates of platelets were assessed by flow cytometry to evaluate the function of platelets in PCP and P-PRP...
September 2017: Experimental and Therapeutic Medicine
Wenjing Yin, Zhengliang Xu, Jiagen Sheng, Xuetao Xie, Changqing Zhang
Erythrocyte sedimentation rate (ESR), which reflects the sedimentation rate of platelets, leukocytes and erythrocytes in response to centrifugal force, may influence the cellular composition of platelet-rich plasma (PRP) obtained via centrifugation methods. However, no relevant studies have substantiated this. In the present study, blood was collected from 40 healthy volunteers and used to prepare PRP with two plasma-based preparation systems [YinPRP and Plasma Rich in Growth Factor (PRGF) systems] and two buffy coat-based systems (RegenPRP and WEGOPRP systems) in a single-donor model...
September 2017: Experimental and Therapeutic Medicine
Siegmund Lang, Marietta Herrmann, Christian Pfeifer, Gero Brockhoff, Johannes Zellner, Michael Nerlich, Peter Angele, Lukas Prantl, Sebastian Gehmert, Markus Loibl
BACKGROUND: Platelet-rich Plasma (PRP) is suggested as xenoprotein-free cell-culture medium replacement for animal-derived supplements. OBJECTIVE: The aim of this study was to investigate PRP-triggered signaling in adipose derived mesenchymal stem cells (ASCs). METHODS: PRP was obtained from 4 male patients. We incubated ASCs in α-MEM with different Platelet derived growth factor (PDGF) subtypes or 10% or 20% pooled PRP or 20% fetal calf serum (FCS) prior to determination of the S-phase fraction (SPF)...
September 11, 2017: Clinical Hemorheology and Microcirculation
Christophe Milants, Olivier Bruyère, Jean-François Kaux
PURPOSE: To evaluate the similarities and differences between the variety of platelet-rich plasma (PRP) formulations, preparation, and uses to try to determine the best responses for the treatment of knee osteoarthritis. MATERIALS AND METHODS: A comparison of the outcomes of randomized controlled trials (RCTs) included in the 3 most recent and high-quality meta-analyses to classify the different studies in 2 groups (bad responders group (BRG) and very good responders group (VGRG))...
2017: BioMed Research International
Franz Hilber, Markus Loibl, Siegmund Lang, Maximilian Kerschbaum, Gero Brockhoff, Peter Angele, Johannes Zellner, Paul Schmitz, Michael Nerlich, Michael Worlicek
INTRODUCTION: The purpose of this study was to evaluate the effect of allogenic leukocyte-reduced platelet-rich plasma on human tenocytes after treatment with prednisolone and to develop a standardization of its application for clinical practice. METHODS: A leukocyte-reduced PRP was produced using the Arthrex Double Syringe (Arthrex, Inc., Naples, FL, USA), in a modified single-spin separation method. Human tenocytes were isolated from discarded rotator cuff segments...
October 2017: Archives of Orthopaedic and Trauma Surgery
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