keyword
MENU ▼
Read by QxMD icon Read
search

Leukocyte rich platelet rich plasma

keyword
https://www.readbyqxmd.com/read/29130636/efficacy-of-subcutaneous-injection-of-platelet-rich-plasma-in-alopecia-a-clinical-and-histological-pilot-study-on-a-rat-model-with-a-six-month-long-term-follow-up-experience
#1
Sophie Orliac, Jean-Michel Serfaty, Anne Perozziello, Olivier Zurlinden, Liliane Louedec, Benjamin Dallaudière
PURPOSE: To assess the potential of platelet-rich plasma (PRP) subcutaneous injection of to treat alopecia and to evaluate local toxicity. MATERIALS-METHODS: Twelve Hairless rats were used. At D0, we performed systematic clinical examination and divided the rat back into four quadrants (Q). We initiated subcutaneous injection using either PRP in PRPQ+, platelet-poor plasma (PPP) in PPPQ+, physiological serum (PS) in PSQ+, or no treatment (Q4). At D7, D14, D21, and D28 but also second month (M2), M3, M4, M5, rats had exactly the same injection procedure...
November 11, 2017: Journal of Cosmetic Dermatology
https://www.readbyqxmd.com/read/29125811/does-leukocyte-poor-or-leukocyte-rich-platelet-rich-plasma-applied-with-biopolymers-have-superiority-to-conventional-platelet-rich-plasma-applications-on-chondrocyte-proliferation
#2
Duygu Yaşar Şirin, İbrahim Yılmaz, Mehmet İsyar, Kadir Öznam, Mahir Mahiroğulları
OBJECTIVES: This study aims to investigate the possible effects of leukocyte concentration in the content of platelet-rich plasma (PRP) and the administration of PRP using a drug delivery system on chondrocyte proliferation in vitro conditions. PATIENTS AND METHODS: Blood from nine male patients (mean age 65 years; range 49 to 81 years) with advanced stage osteoarthritis who had not responded to medical or conservative treatments and underwent total knee arthroplasty was used to prepare two formulations: PRP with low concentration leukocytes (2000-4000 leukocytes/µL) was designated as pure PRP (P-PRP), whereas PRP with high concentration leukocytes (9000-11000 leukocytes/µL) as leukocyte-rich PRP (L-PRP)...
December 2017: Eklem Hastalıkları Ve Cerrahisi, Joint Diseases & related Surgery
https://www.readbyqxmd.com/read/29099894/effects-of-platelet-rich-plasma-and-indomethacin-on-biomechanics-of-rotator-cuff-repair
#3
Molly C Meadows, David M Levy, Christopher M Ferry, Thomas R Gardner, Takeshi Teratani, Christopher S Ahmad
We conducted a study to determine if platelet-rich plasma (PRP) enhances the strength of rotator cuff repair (RCR) and if concomitant use of nonsteroidal anti-inflammatory drugs (NSAIDs) affects PRP efficacy. We also wanted to determine the optimal centrifugation protocol for making PRP from rats. This study used 48 rats, 14 in a centrifugation protocol and 34 in an operative protocol. Six syngeneic rats from the operative group were used as PRP blood donors; the other 28 operative rats underwent bilateral RCRs...
September 2017: American Journal of Orthopedics
https://www.readbyqxmd.com/read/29096373/distribution-recovery-and-concentration-of-platelets-and-leukocytes-in-l-prp-prepared-by-centrifugation
#4
Bruna Alice Gomes de Melo, Andréa Arruda Martins Shimojo, Amanda Gomes Marcelino Perez, José Fabio Santos Duarte Lana, Maria Helena Andrade Santana
Platelet-rich plasma (PRP) is an autologous product prepared from whole blood (WB) that is widely used in regenerative medicine. In clinical practice, discontinuous centrifugation is used for both hand- and machine-prepared PRP. However, separation of WB fractions via centrifugation is a complex process, and the lack of clear mechanisms limits the understanding and evaluation of PRP preparation methods This paper focuses on the distribution, recovery and concentration factor of platelets and leukocytes in L-PRP (leukocyte and platelet-rich plasma) to define a concentration pattern for these blood components due to centrifugation conditions...
October 18, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29058255/platelet-rich-plasma-combinational-treatment-modalities-for-musculoskeletal-conditions
#5
REVIEW
Isabel Andia, Michele Abate
Current research on common musculoskeletal problems, including osteoarticular conditions, tendinopathies, and muscle injuries, focuses on regenerative translational medicine. Platelet-rich plasma therapies have emerged as a potential approach to enhance tissue repair and regeneration. Platelet-rich plasma application aims to provide supraphysiological concentrations of platelets and optionally leukocytes at injured/pathological tissues mimicking the initial stages of healing. However, the efficacy of platelet-rich plasma is controversial in chronic diseases because patients' outcomes show partial improvements...
October 18, 2017: Frontiers of Medicine
https://www.readbyqxmd.com/read/28962125/optimization-of-pure-platelet-rich-plasma-preparation-a-comparative-study-of-pure-platelet-rich-plasma-obtained-using-different-centrifugal-conditions-in-a-single-donor-model
#6
Wenjing Yin, Haitao Xu, Jiagen Sheng, Zhenzhong Zhu, Dongxu Jin, Peichun Hsu, Xuetao Xie, Changqing Zhang
While it has been proved that centrifugal conditions for pure platelet-rich plasma (P-PRP) preparation influence the cellular composition of P-PRP obtained, the optimal centrifugal conditions to prepare P-PRP have not yet been identified. In the present study, platelet-containing plasma (PCP) was prepared with the first-spin of different double-spin methods and P-PRP was prepared with different double-spin methods. Whole-blood analysis was performed to evaluate the cellular composition of PCP and P-PRP. The basal and ADP-induced CD62P expression rates of platelets were assessed by flow cytometry to evaluate the function of platelets in PCP and P-PRP...
September 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28962103/erythrocyte-sedimentation-rate-and-fibrinogen-concentration-of-whole-blood-influences-the-cellular-composition-of-platelet-rich-plasma-obtained-from-centrifugation-methods
#7
Wenjing Yin, Zhengliang Xu, Jiagen Sheng, Xuetao Xie, Changqing Zhang
Erythrocyte sedimentation rate (ESR), which reflects the sedimentation rate of platelets, leukocytes and erythrocytes in response to centrifugal force, may influence the cellular composition of platelet-rich plasma (PRP) obtained via centrifugation methods. However, no relevant studies have substantiated this. In the present study, blood was collected from 40 healthy volunteers and used to prepare PRP with two plasma-based preparation systems [YinPRP and Plasma Rich in Growth Factor (PRGF) systems] and two buffy coat-based systems (RegenPRP and WEGOPRP systems) in a single-donor model...
September 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28922143/leukocyte-reduced-platelet-rich-plasma-stimulates-the-in-vitro-proliferation-of-adipose-tissue-derived-mesenchymal-stem-cells-depending-on-pdgf-signaling
#8
Siegmund Lang, Marietta Herrmann, Christian Pfeifer, Gero Brockhoff, Johannes Zellner, Michael Nerlich, Peter Angele, Lukas Prantl, Sebastian Gehmert, Markus Loibl
BACKGROUND: Platelet-rich Plasma (PRP) is suggested as xenoprotein-free cell-culture medium replacement for animal-derived supplements. OBJECTIVE: The aim of this study was to investigate PRP-triggered signaling in adipose derived mesenchymal stem cells (ASCs). METHODS: PRP was obtained from 4 male patients. We incubated ASCs in α-MEM with different Platelet derived growth factor (PDGF) subtypes or 10% or 20% pooled PRP or 20% fetal calf serum (FCS) prior to determination of the S-phase fraction (SPF)...
September 11, 2017: Clinical Hemorheology and Microcirculation
https://www.readbyqxmd.com/read/28904970/responders-to-platelet-rich-plasma-in-osteoarthritis-a-technical-analysis
#9
REVIEW
Christophe Milants, Olivier Bruyère, Jean-François Kaux
PURPOSE: To evaluate the similarities and differences between the variety of platelet-rich plasma (PRP) formulations, preparation, and uses to try to determine the best responses for the treatment of knee osteoarthritis. MATERIALS AND METHODS: A comparison of the outcomes of randomized controlled trials (RCTs) included in the 3 most recent and high-quality meta-analyses to classify the different studies in 2 groups (bad responders group (BRG) and very good responders group (VGRG))...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28815297/leukocyte-reduced-platelet-rich-plasma-increases-proliferation-of-tenocytes-treated-with-prednisolone-a-cell-cycle-analysis
#10
Franz Hilber, Markus Loibl, Siegmund Lang, Maximilian Kerschbaum, Gero Brockhoff, Peter Angele, Johannes Zellner, Paul Schmitz, Michael Nerlich, Michael Worlicek
INTRODUCTION: The purpose of this study was to evaluate the effect of allogenic leukocyte-reduced platelet-rich plasma on human tenocytes after treatment with prednisolone and to develop a standardization of its application for clinical practice. METHODS: A leukocyte-reduced PRP was produced using the Arthrex Double Syringe (Arthrex, Inc., Naples, FL, USA), in a modified single-spin separation method. Human tenocytes were isolated from discarded rotator cuff segments...
October 2017: Archives of Orthopaedic and Trauma Surgery
https://www.readbyqxmd.com/read/28809630/aplikace-plazmy-obohacen%C3%A3-o-trombocyty-a-leukocyty-k-later%C3%A3-ln%C3%A3-mu-epikondylu-humeru
#11
P Walder, L Paša, L Pavliska
PURPOSE OF THE STUDY The aim of the study was to evaluate the safety and efficacy of the application of platelet- and leukocyte-rich plasma (L-PRP) in the treatment of lateral humeral epicondyle (ERH) as compared to the administration of corticosteroid therapy. MATERIAL AND METHODS It was a prospective, double-blind and randomized clinical trial. It included a total of 25 cases of ERH in 23 patients aged 18 to 60 years. They were divided into two arms: L-PRP arm with 10 cases and corticosteroid arm with 15 cases...
2017: Acta Chirurgiae Orthopaedicae et Traumatologiae Cechoslovaca
https://www.readbyqxmd.com/read/28805478/production-of-platelet-rich-plasma-gel-from-elderly-patients-under-antithrombotic-drugs-perspectives-in-chronic-wounds-care
#12
M Velier, J Magalon, A Daumas, M Cassar, P Francois, A Ghazouane, C Philandrianos, B Bertrand, C Frere, D Bernot, P Villani, F Dignat George, F Sabatier
Platelet-Rich Plasma (PRP) is an autologous biological therapy obtained by centrifuging the patient's own blood to concentrate platelets. The addition of autologous thrombin and calcium chloride to PRP allows the production of a semi-solid form called PRP gel. PRP gel is increasingly used in a variety of tissue defects and predominantly in the management of non-healing chronic wounds. The topical application of PRP gel seems promising due to the capability of platelets to store and secrete growth factors (GF), fibrin and cytokines, which are essentials for wound healing...
August 14, 2017: Platelets
https://www.readbyqxmd.com/read/28761774/proinflammatory-and-anabolic-gene-expression-effects-of-platelet-rich-gel-supernatants-on-equine-synovial-membrane-explants-challenged-with-lipopolysaccharide
#13
Jorge U Carmona, Diana L Ríos, Catalina López, María E Álvarez, Jorge E Pérez
Platelet-rich plasma (PRP) preparations are used in horses with osteoarthritis (OA). However, some controversies remain regarding the ideal concentration of platelets and leukocytes to produce an adequate anti-inflammatory and anabolic response in the synovial membrane. The aims of this study were to study the influence of leukoconcentrated platelet-rich gel (Lc-PRG) and leukoreduced platelet-rich gel (Lr-PRG) supernatants on the quantitative expression of some proinflammatory and anabolic genes in equine synovial membrane explants (SMEs) challenged with lipopolysaccharide (LPS)...
2017: Veterinary Medicine International
https://www.readbyqxmd.com/read/28758836/contributions-for-classification-of-platelet-rich-plasma-proposal-of-a-new-classification-marspill
#14
Jose Fabio Santos Duarte Lana, Joseph Purita, Christian Paulus, Stephany Cares Huber, Bruno Lima Rodrigues, Ana Amélia Rodrigues, Maria Helena Santana, João Lopo Madureira, Ângela Cristina Malheiros Luzo, William Dias Belangero, Joyce Maria Annichino-Bizzacchi
Platelet-rich plasma (PRP) has emerged as a significant therapy used in medical conditions with heterogeneous results. There are some important classifications to try to standardize the PRP procedure. The aim of this report is to describe PRP contents studying celular and molecular components, and also propose a new classification for PRP. The main focus is on mononuclear cells, which comprise progenitor cells and monocytes. In addition, there are important variables related to PRP application incorporated in this study, which are the harvest method, activation, red blood cells, number of spins, image guidance, leukocytes number and light activation...
July 2017: Regenerative Medicine
https://www.readbyqxmd.com/read/28648869/long-term-cytokine-and-growth-factor-release-from-equine-platelet-rich-fibrin-clots-obtained-with-two-different-centrifugation-protocols
#15
Román F Jiménez-Aristizabal, Catalina López, María E Álvarez, Carlos Giraldo, Marta Prades, Jorge U Carmona
OBJECTIVES: To compare the temporal release (over three weeks) of tumor necrosis factor alpha (TNF-α), interleukin 4 (IL-4), IL-1 receptor antagonist (IL-1ra), platelet-derived growth factor BB (PDGF-BB) and transforming growth factor beta-1 (TGF-β1) from two platelet-rich fibrin (PRF) preparations from equine blood obtained at either 240g/8min or 416g/10min. METHODS: Whole blood from 10 horses was used to obtain PRF clots by two different centrifugation protocols...
September 2017: Cytokine
https://www.readbyqxmd.com/read/28635382/white-cell-and-platelet-content-affects-the-release-of-bioactive-factors-in-different-blood-derived-scaffolds
#16
S Cabaro, V D'Esposito, R Gasparro, F Borriello, F Granata, G Mosca, F Passaretti, J C Sammartino, F Beguinot, G Sammartino, P Formisano, F Riccitiello
Platelet-derived factors are biomaterials that might accelerate healing process in oral, maxillofacial, and several other applications. Release of specific factors by platelet concentrates is critical to achieving a successful outcome. Here, we have shown that platelet-rich fibrin (PRF) clots were beneficial sources of leukocytes, which may directly affect the release of chemokines and growth factors. When compared with the standard leukocyte-PRF (L-PRF), the experimental low-force modified procedure [defined as advanced-PRF (A-PRF)] entrapped the same content of viable leukocytes, released a similar amount of inflammatory cytokines, but secreted 3-, 1...
June 21, 2017: Platelets
https://www.readbyqxmd.com/read/28560233/evolution-current-status-and-advances-in-application-of-platelet-concentrate-in-periodontics-and-implantology
#17
REVIEW
Amit Arvind Agrawal
Platelet concentrates (PC) [platelet-rich plasma (PRP) and platelet-rich fibrin (PRF)] are frequently used for surgical procedures in medical and dental fields, particularly in oral and maxillofacial surgery, plastic surgery and sports medicine. The objective of all these technologies is to extract all the elements from a blood sample that could be used to improve healing and promote tissue regeneration. Although leukocyte rich and leukocyte poor PRP's have their own place in literature, the importance of non-platelet components in a platelet concentrate remains a mystery...
May 16, 2017: World Journal of Clinical Cases
https://www.readbyqxmd.com/read/28520812/the-effect-of-platelet-rich-plasma-on-osseous-healing-in-dogs-undergoing-high-tibial-osteotomy
#18
Samuel P Franklin, Emily E Burke, Shannon P Holmes
OBJECTIVES: The purpose of this study was to investigate whether platelet-rich plasma (PRP) enhances osseous healing in conjunction with a high tibial osteotomy in dogs. STUDY DESIGN: Randomized controlled trial. METHODS: Sixty-four client-owned pet dogs with naturally occurring rupture of the anterior cruciate ligament and that were to be treated with a high tibial osteotomy (tibial plateau leveling osteotomy) were randomized into the treatment or control group...
2017: PloS One
https://www.readbyqxmd.com/read/28509340/prf-based-matrices-to-improve-angiogenesis-in-an-in-vitro-co-culture-model-for-bone-tissue-engineering
#19
Eva Dohle, Karima El Bagdadi, Robert Sader, Joseph Choukroun, C James Kirkpatrick, Shahram Ghanaati
In the context of prevascularization strategies for tissue engineering purposes, co-culture systems consisting of outgrowth endothelial cells (OECs) and primary osteoblasts (pOBs) have been established as a promising in vitro tool to study regeneration mechanisms and to identify factors that might positively influence repair processes like wound healing or angiogenesis. The development of autologous injectable platelet rich fibrin (PRF), which can be generated from peripheral blood in a minimal invasive procedure, fulfils several requirements for clinically applicable cell-based tissue engineering strategies...
May 16, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28503993/dual-inhibition-of-pi3k-and-mtor-by-vs-5584-suppresses-thrombus-formation
#20
Thomas Später, Isabelle Müller, Hermann Eichler, Michael D Menger, Matthias W Laschke, Emmanuel Ampofo
VS-5584 is a highly selective dual kinase inhibitor which suppresses phosphatidylinositol 3-kinase (PI3K) and mammalian target of rapamycin (mTOR) activity. Because these kinases are crucially involved in primary hemostasis, we herein investigated the effect of this compound on thrombus formation in vitro and in vivo. Pretreatment of washed platelets (WP) or platelet-rich plasma (PRP) with VS-5584 inhibited the agonist-induced activation of surface glycoprotein complex (GP)IIb/IIIa and the upregulation of P-selectin...
May 15, 2017: Platelets
keyword
keyword
17989
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"