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https://www.readbyqxmd.com/read/28086834/detection-of-circulating-tumor-cells-with-ck20-rt-pcr-is-an-independent-negative-prognostic-marker-in-colon-cancer-patients-a-prospective-study
#1
Sebastian Hinz, Alexander Hendricks, Amke Wittig, Clemens Schafmayer, Jürgen Tepel, Holger Kalthoff, Thomas Becker, Christian Röder
BACKGROUND: Detection of circulating (CTC) or disseminated tumor cells (DTC) has been associated with negative prognosis and outcome in patients with colorectal cancer, though testing for these cells is not yet part of clinical routine. There are several different methodological approaches to detect tumor cells but standardized detection assays are not implemented so far. METHODS: In this prospective monocentric study 299 patients with colon cancer were included...
January 13, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28079185/high-efficiency-tri-band-quasi-continuous-phase-gradient-metamaterials-based-on-spoof-surface-plasmon-polaritons
#2
Yongfeng Li, Hua Ma, Jiafu Wang, Yongqiang Pang, Qiqi Zheng, Hongya Chen, Yajuan Han, Jieqiu Zhang, Shaobo Qu
A high-efficiency tri-band quasi-continuous phase gradient metamaterial is designed and demonstrated based on spoof surface plasmon polaritons (SSPPs). High-efficiency polarizaiton conversion transmission is firstly achieved via tailoring phase differece between the transmisive SSPP and the space wave in orthogonal directions. As an example, a tri-band circular-to-circular (CTC) polarization conversion metamateiral (PCM) was designed by a nonlinearly dispersive phase difference. Using such PCM unit cell, a tri-band quasi-continuous phase gradient metamaterial (PGM) was then realized by virtue of the Pancharatnam-Berry phase...
January 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28075552/nanoparticles-coated-with-neutrophil-membranes-can-effectively-treat-cancer-metastasis
#3
Ting Kang, Qianqian Zhu, Dan Wei, Jingxian Feng, Jianhui Yao, Tianze Jiang, Qingxiang Song, Xunbin Wei, Hong-Zhuan Chen, Xiaoling Gao, Jun Chen
The dissemination, seeding and colonization of circulating tumor cells (CTC) serves as the root of distant metastasis. As a key step in the early stage of metastasis formation, colonization of CTC in the (pre-) metastatic niche appears to be a valuable target. Evidence showed that inflammatory neutrophils possess both CTC- and niche-targeting property by the intrinsic cell adhesion molecules on neutrophils. Inspired by this mechanism, we developed a nanosize neutrophil-mimicking drug delivery system (NM-NP) by coating neutrophils membranes on the surface of poly (latic-co-glycolic acid) nanoparticles (NP)...
January 11, 2017: ACS Nano
https://www.readbyqxmd.com/read/28073899/paper-based-microrna-expression-profiling-from-plasma-and-circulating-tumor-cells
#4
Sai Mun Leong, Karen Mei-Ling Tan, Hui Wen Chua, Mo-Chao Huang, Wai Chye Cheong, Mo-Huang Li, Steven Tucker, Evelyn Siew-Chuan Koay
BACKGROUND: Molecular characterization of circulating tumor cells (CTCs) holds great promise for monitoring metastatic progression and characterizing metastatic disease. However, leukocyte and red blood cell contamination of routinely isolated CTCs makes CTC-specific molecular characterization extremely challenging. METHODS: Here we report the use of a paper-based medium for efficient extraction of microRNAs (miRNAs) from limited amounts of biological samples such as rare CTCs harvested from cancer patient blood...
January 10, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28070168/circulating-tumor-microemboli-ctm-and-vimentin-circulating-tumor-cells-ctcs-detected-by-a-size-based-platform-predict-worse-prognosis-in-advanced-colorectal-cancer-patients-during-chemotherapy
#5
Dejun Zhang, Lei Zhao, Pengfei Zhou, Hong Ma, Fang Huang, Min Jin, Xiaomeng Dai, Xiumei Zheng, Shaoyi Huang, Tao Zhang
BACKGROUND: Circulating tumor cells (CTCs) detected in peripheral blood (PB) of cancer patients can be identified as isolated CTCs and circulating tumor microemboli (CTM). This study aimed to evaluate the prognostic value of CTM detection and CTC phenotype in advanced colorectal cancer (CRC) patients during chemotherapy. METHODS: A size-based platform for CTC isolation was applied. PB samples (5 ml) from 98 advanced CRC patients during 2-6 cycles chemotherapy were collected for CTC detection, and CTC count was correlated to patient's clinicopathological characteristics and clinical outcome...
2017: Cancer Cell International
https://www.readbyqxmd.com/read/28069017/dose-escalation-of-stereotactic-body-radiotherapy-sbrt-for-locally-advanced-unresectable-pancreatic-cancer-patients-with-cyberknife-protocol-of-a-phase-i-study
#6
Shui-Wang Qing, Xiao-Ping Ju, Yang-Sen Cao, Huo-Jun Zhang
BACKGROUND: Dose escalation of SBRT for locally advanced pancreatic cancer patients had been reported in several studies in one or three fractions, and phase I protocol was developed to investigate the maximum tolerated dose with CyberKnife for locally advanced unresectable pancreatic cancer patients in five fractions. METHODS: The study is designed as a mono-center phase I study. The primary endpoint is to determine the maximum tolerated dose by frequency of III/IV GI (gastrointestinal) toxicity...
January 9, 2017: Radiation Oncology
https://www.readbyqxmd.com/read/28060956/technical-insights-into-highly-sensitive-isolation-and-molecular-characterization-of-fixed-and-live-circulating-tumor-cells-for-early-detection-of-tumor-invasion
#7
Sophie Laget, Lucile Broncy, Katia Hormigos, Dalia M Dhingra, Fatima BenMohamed, Thierry Capiod, Magne Osteras, Laurent Farinelli, Stephen Jackson, Patrizia Paterlini-Bréchot
Circulating Tumor Cells (CTC) and Circulating Tumor Microemboli (CTM) are Circulating Rare Cells (CRC) which herald tumor invasion and are expected to provide an opportunity to improve the management of cancer patients. An unsolved technical issue in the CTC field is how to obtain highly sensitive and unbiased collection of these fragile and heterogeneous cells, in both live and fixed form, for their molecular study when they are extremely rare, particularly at the beginning of the invasion process. We report on a new protocol to enrich from blood live CTC using ISET® (Isolation by SizE of Tumor/Trophoblastic Cells), an open system originally developed for marker-independent isolation of fixed tumor cells...
2017: PloS One
https://www.readbyqxmd.com/read/28059522/dual-functional-lipid-coating-for-nanopillar-based-capture-of-circulating-tumor-cells-with-high-purity-and-efficiency
#8
Hsin-Ya Lou, Wenting Zhao, Lindsey Hanson, Connie Zeng, Yi Cui, Bianxiao Cui
Clinical studies of circulating tumor cells (CTC) have stringent demands for high capture purity and high capture efficiency. Nanostructured surfaces have been shown to significantly increase the capture efficiency, yet suffer from low capture purity. Here we introduced a dual functional lipid coating on nanostructured surfaces. The lipid coating serves both as an effective passivation layer that helps prevent non-specific cell adhesion, and as a functionalized layer for antibody-based specific cell capture...
January 6, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28058627/a-surface-tension-magnetophoretic-device-for-rare-cell-isolation-and-characterization
#9
Emma E van der Toom, James E Verdone, Changhan Jun, Doru Petrisor, Sunghwan Lim, Jean J M C H de la Rosette, Theo M de Reijke, Michael A Gorin, Kenneth J Pienta, Dan Stoianovici
The cancer community continues to search for an efficient and cost-effective technique to isolate and characterize circulating cells (CTCs) as a 'real-time liquid biopsy'. Existing methods to isolate and analyze CTCs require various transfer, wash, and staining steps that can be time consuming, expensive, and led to the loss of rare cells. To overcome the limitations of existing CTC isolation strategies, we have developed an inexpensive 'lab on a chip' device for the enrichment, staining, and analysis of rare cell populations...
February 2017: Medical Oncology
https://www.readbyqxmd.com/read/28039713/value-of-circulating-tumor-cells-positive-for-thyroid-transcription-factor-1-ttf-1-to-predict-recurrence-and-survival-rates-for-endometrial-carcinoma
#10
Yan Zhang, Xin Qu, Peng Peng Qu
PURPOSE: This study was conducted to analyze the predictive value of circulating tumor cells (CTC) expressing thyroid transcription factor-1 (TTF-1) on the recurrence and survival rates of endometrial carcinoma patients treated with laparoscopic surgery. METHODS: 78 patients were recruited, diagnosed with endometrial carcinoma and measured CTC expressing TTF-1 using flow cytometry in blood and tissues. Then, the patients were distributed into TTF-1-positive (N=42) and -negative (N=36) groups...
November 2016: Journal of B.U.ON.: Official Journal of the Balkan Union of Oncology
https://www.readbyqxmd.com/read/28039472/analysis-of-circulating-tumor-cells-from-lung-cancer-patients-with-multiple-biomarkers-using-high-performance-size-based-microfluidic-chip
#11
Wanlei Gao, Haojun Yuan, Fengxiang Jing, Shan Wu, Hongbo Zhou, Hongju Mao, Qinghui Jin, Jianlong Zhao, Hui Cong, Chunping Jia
Circulating tumor cells (CTCs) have attracted pretty much attention from scientists because of their important relationship with the process of metastasis. Here, we developed a size-based microfluidic chip containing triangular pillar array and filter channel array for detecting single CTCs and CTC clusters independent of tumor-specific markers. The cell populations in chip were characterized by immune-fluorescent staining combining an epithelial marker and a mesenchymal marker. We largely decreased the whole time of detection process to nearly 1...
December 26, 2016: Oncotarget
https://www.readbyqxmd.com/read/28035320/clinical-significance-of-early-changes-in%C3%A2-circulating-tumor-cells-from-patients-receiving-first-line-cisplatin-based-chemotherapy-for-metastatic-urothelial-carcinoma
#12
Emanuela Fina, Andrea Necchi, Patrizia Giannatempo, Maurizio Colecchia, Daniele Raggi, Maria Grazia Daidone, Vera Cappelletti
Background: The therapeutic paradigm of metastatic urothelial carcinoma (UC) is rapidly shifting and new biomarkers are needed to enhance patient selection. Objective: Early identification of dynamic predictors of outcome may be a key to optimize the sequence of effective therapies in metastatic UC patients. Methods: Blood samples from patients receiving first-line MVAC chemotherapy were collected at baseline (T0) and after 2 cycles (T2). Samples were processed by immunomagnetic beads (AdnaTest ProstateCancerSelect kit) and the expression of EPCAM, MUC1 and ERBB2 was studied using multiplex-PCR...
October 27, 2016: Bladder Cancer
https://www.readbyqxmd.com/read/28030836/expression-and-clinical-relevance-of-epithelial-and-mesenchymal-markers-in-circulating-tumor-cells-from-colorectal-cancer
#13
Ren Zhao, Zhen Cai, Sheng Li, Yong Cheng, Hua Gao, Fang Liu, Shiyang Wu, Suyan Liu, Yan Dong, Lei Zheng, Wenbin Zhang, Xiaojun Wu, Xueqing Yao
Circulating tumor cells (CTCs) with phenotypic hallmarks of epithelial-mesenchymal transition (EMT) reportedly contribute to tumor metastasis in different cancer types. We therefore evaluated the expression of EMT markers in CTCs obtained from a large cohort of Chinese patients with colorectal cancer (CRC) and investigated their clinical relevance. The CanPatrolTM CTC enrichment technique was used to isolate and classify CTCs. CTCs were detected in 1046 of 1203 patients (86.9%), and three phenotypes were identified based on the expression of epithelial and mesenchymal markers: epithelial CTCs, biophenotypic (epithelial/mesenchymal) CTCs, and mesenchymal CTCs...
December 21, 2016: Oncotarget
https://www.readbyqxmd.com/read/28029660/phenotypic-characterization-of-circulating-tumor-cells-in-triple-negative-breast-cancer-patients
#14
Sofia Agelaki, Melina Dragolia, Harris Markonanolaki, Saad Alkahtani, Christos Stournaras, Vassilis Georgoulias, Galatea Kallergi
INTRODUCTION: Patients with triple negative breast cancer (TNBC), are considered as a poor prognosis group for whom no targeted therapies are currently available. The aim of the present study was to phenotypically characterize their CTCs in order to explore potential therapeutic targets. METHODS: PBMC's cytospins were prepared from 45 early (before and after adjuvant chemotherapy), 10 metastatic TNBC and 21 hormone receptor (HR) -positive patients. The expression of Cytokeratins (CK), ER, PR, EGFR and HER2 on CTCs was assessed using immunofluoresence staining and ARIOL analysis...
December 24, 2016: Oncotarget
https://www.readbyqxmd.com/read/28028983/monitoring-of-circulating-tumor-cells-by-a-combination-of-immunomagnetic-enrichment-and-rt-pcr-in-colorectal-cancer-patients-undergoing-surgery
#15
Gabriela Vojtechova, Lucie Benesova, Barbora Belsanova, Petra Minarikova, Miroslav Levy, Ludmila Lipska, Stepan Suchanek, Miroslav Zavoral, Marek Minarik
BACKGROUND: The presence of circulating tumor cells (CTC) has been reported in patients with advanced colorectal cancer. Monitoring CTC (also known as a liquid-biopsy) has recently become the center of interest for low-invasive monitoring of cancer progression and predictive biomarkers testing. Along with high-cost technology and a complex methodology, a straightforward method based on magnetic beads enrichment followed by RT-PCR is set to allow for routine CTC analysis in colorectal cancer patients...
November 2016: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
https://www.readbyqxmd.com/read/28028372/long-term-prognostic-impact-of-circulating-tumour-cells-in-gastric-cancer-patients
#16
Hiroaki Ito, Jun Sato, Yukio Tsujino, Noriko Yamaguchi, Satoshi Kimura, Keigo Gohda, Katsuhiro Murakami, Manabu Onimaru, Tohru Ohmori, Fumihiro Ishikawa, Haruhiro Inoue
AIM: To analyse the long-term prognostic impact of circulating tumour cells (CTCs) in gastric cancer patients who underwent surgery. METHODS: A 7.5-mL peripheral vein blood sample was obtained from each patient with treatment-negative gastric adenocarcinoma before surgery. OBP-401, a telomerase-specific, replication-selective, oncolytic adenoviral agent carrying the green fluorescent protein gene, was used to label CTCs. Correlations between the number of CTCs and clinical end points were evaluated...
December 14, 2016: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28012133/ctc-clusters-in-cancer-progression-and-metastasis
#17
REVIEW
Anna Fabisiewicz, Ewa Grzybowska
Circulating tumor cells (CTC) in the blood of cancer patients are regarded as potential metastatic seeds, and their detailed characterization holds great promises for more exact prognosis, better diagnosis and therapy of the metastatic cancer. Circulating tumor cell clusters represent different class of CTCs, with specific properties, including high metastatic potential. In this review, we present current opinions on differences between single CTCs and CTC clusters, their mode of dissemination, methods of detection and clinical importance in various types of cancer...
January 2017: Medical Oncology
https://www.readbyqxmd.com/read/28011487/detection-of-circulating-tumour-cells-and-survival-of-patients-with-non-small-cell-lung-cancer
#18
Dimple Chudasama, James Barr, Julie Beeson, Emma Beddows, Niall McGonigle, Alexandra Rice, Andrew Nicholson, Vladimir Anikin
BACKGROUND: Detection of circulating tumour cells (CTCs) in the peripheral blood of lung cancer patients may predict survival. Various platforms exist that allow capture of these cells for further analysis; little work however, has been done with the ScreenCell device, an antibody-independent CTC platform. The aim of our study was to evaluate the ScreenCell device for detection of CTCs in lung cancer patients and to establish correlations of these findings with survival. MATERIALS AND METHODS: Twenty-three patients, nine males, and fourteen females, underwent surgical treatment from February to May 2014 for non-small cell lung cancer...
January 2017: Anticancer Research
https://www.readbyqxmd.com/read/28009470/proportion-of-circulating-tumor-cell-clusters-increases-during-cancer-metastasis
#19
Yuanzhen Suo, Chengying Xie, Xi Zhu, Zhichao Fan, Zhangru Yang, Hao He, Xunbin Wei
Circulating tumor cell (CTC) clusters are found among CTCs and show significantly greater potential for an important role in cancer metastasis than single CTCs, which have been traditionally believed as the majority of CTCs. The accurate proportion and dynamics of CTC clusters remain unclear due to the fact that CTCs in blood flow are very difficult to detect in vivo and in vitro. CTC clusters are even more difficult to be distinguished from CTCs without perturbation by state-of-the-art detection methods. Here, we demonstrate that by using in vivo flow cytometry (IVFC), we can reliably measure the proportion and dynamics of CTC clusters in two murine tumor models...
December 23, 2016: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28009094/clinical-value-of-mri-detected-extramural-venous-invasion-in-rectal-cancer
#20
REVIEW
Pratik Tripathi, Sheng Xiang Rao, Meng Su Zeng
OBJECTIVE: This study highlights the importance of MRI-detected EMVI (mrEMVI) before and after rectal cancer treatment. In addition, it analyzes the prognostic correlation between mrEMVI and circulating tumor cells (CTCs) in rectal cancer. METHODS AND MATERIALS: We reviewed literature in English from 1981 to 2016 on Medline (Ovid), Embase (Ovid), and Cochrane. Keywords used in the literature search were as follows: magnetic resonance imaging/MRI; extramural venous invasion/EMVI; rectal cancer; CTC; colorectal cancer...
December 23, 2016: Journal of Digestive Diseases
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