keyword
MENU ▼
Read by QxMD icon Read
search

Leukocyte rich

keyword
https://www.readbyqxmd.com/read/29761887/telomere-length-and-genetic-variations-affecting-telomere-length-as-biomarkers-for-facial-regeneration-with-platelet-rich-fibrin-based-on-the-low-speed-centrifugation-concept
#1
Cleopatra Nacopoulos, Kalliopi Gkouskou, Dimitrios Karypidis, Ioannis Vlastos, Anna-Maria Vesala, Joseph Choukroun, Richard J Miron, Emmanuel Prokopakis
BACKGROUND: Platelet-Rich Fibrin (PRF), a fibrin matrix produced by single blood centrifugation that contains leukocytes, platelets, and growth factors, is increasingly being utilized for facial regeneration purposes. However, our understanding of the involved pathophysiological mechanisms affecting regeneration is limited and current protocols require better optimization. Biomarkers that are related to skin aging such as telomere length (TL) have been proposed as a mean to analyze patients' stratification...
May 15, 2018: Journal of Cosmetic Dermatology
https://www.readbyqxmd.com/read/29760073/regulation-of-myeloid-cell-phagocytosis-by-lrrk2-via-wave2-complex-stabilization-is-altered-in-parkinson-s-disease
#2
Kwang Soo Kim, Paul C Marcogliese, Jungwoo Yang, Steve M Callaghan, Virginia Resende, Elizabeth Abdel-Messih, Connie Marras, Naomi P Visanji, Jana Huang, Michael G Schlossmacher, Laura Trinkle-Mulcahy, Ruth S Slack, Anthony E Lang, David S Park
Leucine-rich repeat kinase 2 ( LRRK2 ) has been implicated in both familial and sporadic Parkinson's disease (PD), yet its pathogenic role remains unclear. A previous screen in Drosophila identified Scar/WAVE (Wiskott-Aldrich syndrome protein-family verproline) proteins as potential genetic interactors of LRRK2 Here, we provide evidence that LRRK2 modulates the phagocytic response of myeloid cells via specific modulation of the actin-cytoskeletal regulator, WAVE2. We demonstrate that macrophages and microglia from LRRK2-G2019S PD patients and mice display a WAVE2-mediated increase in phagocytic response, respectively...
May 14, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29730556/new-peptide-my1340-revert-the-inhibition-effect-of-vegf-on-dendritic-cells-differentiation-and-maturation-via-blocking-vegf-nrp-1-axis-and-inhibit-tumor-growth-in-vivo
#3
Zheng Mo, Fei Yu, Su Han, Songhua Yang, Liangliang Wu, Peng Li, Shunchang Jiao
The development and clinical application of immunostimulatory therapy provides us a new and exciting strategy in cancer treatment of which the agents act on crucial receptors. Given the fact that Neuropilin-1(NRP-1) is essential for vascular endothelial growth factor (VEGF) to inhibit LPS-dependent maturation of dendritic cells (DCs), it may present a potentially meaningful target in cancer immunotherapy. To explore this hypothesis, we synthesized a novel polypeptide called MY1340 consist of 32 amino acids with the aim of targeting VEGF-NRP-1 axis...
May 3, 2018: International Immunopharmacology
https://www.readbyqxmd.com/read/29722762/prp-as-an-adjunct-to-rotator-cuff-tendon-repair
#4
F Alan Barber
Arthroscopic rotator cuff repair is a commonly performed repair. Technical developments provide surgeons the tools to create biomechanically robust repairs. How can the biological response mirror the strong and stable surgery? Platelet-rich plasma (PRP) is a supraphysiological platelet concentration which may positively augment rotator cuff healing. Not all PRPs are the same. High leukocyte levels and thrombin activation may be detrimental to tendon healing. Thrombin activation triggers an immediate release of growth factors and may actually inhibit some parts of the healing response...
June 2018: Sports Medicine and Arthroscopy Review
https://www.readbyqxmd.com/read/29719011/short-term-storage-of-platelet-rich-plasma-at-room-temperature-does-not-affect-growth-factor-or-catabolic-cytokine-concentration
#5
Brooke H Wilson, Brian J Cole, Margaret B Goodale, Lisa A Fortier
The aim of this study was to provide clinical recommendations about the use of platelet-rich plasma (PRP) that was subjected to short-term storage at room temperature. We determined bioactive growth factor and cytokine concentrations as indicators of platelet and white blood cell degranulation in blood and PRP. Additionally, this study sought to validate the use of manual, direct smear analysis as an alternative to automated methods for platelet quantification in PRP. Blood was used to generate low-leukocyte PRP (Llo PRP) or high-leukocyte PRP (Lhi PRP)...
April 2018: American Journal of Orthopedics
https://www.readbyqxmd.com/read/29695061/use-of-leukocyte-platelet-l-prf-rich-fibrin-in-diabetic-foot-ulcer-with-osteomyelitis-three-clinical-cases-report
#6
Alessandro Crisci, Giuseppe Marotta, Anna Licito, Edda Serra, Giulio Benincasa, Michela Crisci
In this study, the use of fibrin rich in leukocytes and platelets (L-PRF) was explored to heal osteomyelitis ulcers in a diabetic foot. The goal was to standardize the utilization of L-PRF in patients with osteomyelitis to direct it for healing. L-PRF was obtained autologously from the peripheral blood of the diabetic patients ( n = 3) having osteomyelitis and skin lesions for at least six months. The L-PRF and supernatant serum were inserted into the skin lesion to the bone after a surgical debridement. The evolution of lesions over time was analyzed...
April 24, 2018: Diseases (Basel)
https://www.readbyqxmd.com/read/29684380/sustained-or-higher-levels-of-growth-factors-in-platelet-rich-plasma-during-7-day-storage
#7
Ying-Hao Wen, Wan-Ying Lin, Chi-Jui Lin, Yu-Chen Sun, Pi-Yueh Chang, Hsin-Yao Wang, Jang-Jih Lu, Wen-Lin Yeh, Tzong-Shi Chiueh
BACKGROUND: The effectiveness of platelet-rich plasma (PRP) for treating soft tissue injuries is still controversial. Most of PRPs were prepared simply by concentrating in volume and were injected right after preparation in physician offices. Neither platelet count nor growth factors were quantitated in advance. We prepared and stored leukocyte and platelet-rich plasma (L-PRP) by regular separation protocols for blood components in the blood bank. And we investigated the dynamic change of growth factors in the L-PRPs over the period of storage...
April 20, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/29680514/brain-blood-cerebrospinal-fluid-and-serum-biomarkers-in-schizophrenia
#8
REVIEW
Alireza Mohammadi, Ehsan Rashidi, Vahid Ghasem Amooeian
Over the last decade, finding a reliable biomarker for the early detection of schizophrenia (Scz) has been a topic of interest. The main goal of the current review is to provide a comprehensive view of the brain, blood, cerebrospinal fluid (CSF), and serum biomarkers of Scz disease. Imaging studies have demonstrated that the volumes of the corpus callosum, thalamus, hippocampal formation, subiculum, parahippocampal gyrus, superior temporal gyrus, prefrontal and orbitofrontal cortices, and amygdala-hippocampal complex were reduced in patients diagnosed with Scz...
April 13, 2018: Psychiatry Research
https://www.readbyqxmd.com/read/29670110/tumor-associated-macrophages-promote-progression-and-the-warburg-effect-via-ccl18-nf-kb-vcam-1-pathway-in-pancreatic-ductal-adenocarcinoma
#9
Huilin Ye, Quanbo Zhou, Shangyou Zheng, Guolin Li, Qing Lin, Lusheng Wei, Zhiqiang Fu, Bin Zhang, Yimin Liu, Zhihua Li, Rufu Chen
Tumor-associated macrophages (TAMs) are frequently found near pancreatic cancer cells, but it is uncertain whether they are involved in pancreatic cancer progression and the Warburg effect. Here, we show that CCL18 secreted by TAMs facilitates malignant progression and induced a glycolytic phenotype in pancreatic cancer, partially owing to paracrine induction of VCAM-1 in pancreatic cancer cells. Reciprocally, VCAM-1-induced lactate production from pancreatic cancer cells with enhanced aerobic glycolysis activates macrophages to a TAM-like phenotype, forming a positive feedback loop...
April 18, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29662262/treatment-of-endo-periodontal-lesion-using-leukocyte-platelet-rich-fibrin-a-case-report
#10
Pablo Betancourt, Ricardo Elgueta, Ramon Fuentes
Case Description: The main objective of this paper was to report the clinical effectiveness of leukocyte- platelet- rich fibrin (L-PRF) in the treatment of a combined endo-periodontal lesion of an upper first premolar. Clinical Findings: The tooth had a profound abfraction on the vestibular aspect and presented no mobility but revealed a deep pocket measuring of 11 mm on the mesial vestibular aspect and 14 mm on the mesial palatine aspect. The three dimensional image analysis showed total bone loss in the mesial aspect and an extensively bone loss of the vestibular aspect of the vestibular root...
December 30, 2017: Colombia Médica: CM
https://www.readbyqxmd.com/read/29655569/activation-of-the-porcine-alveolar-macrophages-via-toll-like-receptor-4-nf-%C3%AE%C2%BAb-mediated-pathway-provides-a-mechanism-of-resistin-leading-to-inflammation
#11
Bi Li, Jing Fang, Zhicai Zuo, Sirui Yin, Tingting He, Mingxian Yang, Junliang Deng, Liuhong Shen, Xiaoping Ma, Shumin Yu, Ya Wang, Zhihua Ren, Hengmin Cui
Resistin, a previously discovered cysteine-rich adipokine known to regulate glucose metabolism, has been emerged as a mediator in inflammation and immunity. Its level was supposed to be related to the expression of indicators, such as interleukin-1β (IL-1β), IL-6 and tumor necrosis factor-α (TNF-α) in inflammation. Toll-like receptor 4 (TLR4) was reported to be a receptor for resistin in cells, like leukocytes and peripheral blood mononuclear cells (PBMC). However, the pro-inflammatory role of resistin and its intracellular mechanisms in alveolar macrophages have not been thoroughly validated...
April 11, 2018: Cytokine
https://www.readbyqxmd.com/read/29629438/influence-of-leukocyte-and-platelet-rich-fibrin-l-prf-on-the-outcomes-of-impacted-mandibular-third-molar-removal-surgery-a-split-mouth-randomized-clinical-trial
#12
Povilas Daugela, Vaidas Grimuta, Dalius Sakavicius, Juozas Jonaitis, Gintaras Juodzbalys
OBJECTIVES: The purpose of this study was to evaluate the influence of leukocyte- and platelet-rich fibrin (L-PRF) on impacted mandibular third molar (IMTM) extraction wound healing, patient postoperative discomfort, and incidence of alveolar osteitis. METHOD AND MATERIALS: Thirty-four patients (20 female, 14 male) who met the inclusion criteria for this split-mouth randomized clinical trial were enrolled and 30 patients completed the study. Patients were randomized and underwent bilateral IMTM surgical extractions...
2018: Quintessence International
https://www.readbyqxmd.com/read/29566102/facilitated-recruitment-of-mesenchymal-stromal-cells-by-bone-marrow-concentrate-and-platelet-rich-plasma
#13
Hannah L Holmes, Brooke Wilson, Julian P Goerger, Jesse L Silverberg, Itai Cohen, Warren R Zipfel, Lisa A Fortier
BACKGROUND: Biologics containing growth factors are frequently used to enhance healing after musculoskeletal injuries. One mechanism of action is thought to be though the ability of biologics to induce homing and migration of endogenous mesenchymal stromal cells (MSCs) to a target tissue. However, the ability of biologics to stimulate chemotaxis (directed migration of cells) and chemokinesis (increase rate of cell migration) of MSCs is unknown. HYPOTHESIS/PURPOSE: The aim of this study was to directly compare the ability of biologics including platelet rich plasma (PRP) and bone marrow concentrate (BMC) to induce MSC migration...
2018: PloS One
https://www.readbyqxmd.com/read/29556269/leukocyte-depletion-does-not-affect-the-in-vitro-healing-ability-of-platelet-rich-plasma
#14
Ilaria Giusti, Marianna Di Francesco, Sandra D'Ascenzo, Paola Palumbo, Anna Rughetti, Luigi Dell'Orso, Paola Annunziata Varasano, Gabriella Lucidi Pressanti, Vincenza Dolo
The clinical use of platelet-rich plasma (PRP) containing or deprived of leukocytes remains a subject of debate and a controversial issue. It is not yet clear whether leukocyte content has a positive or negative effect on tissue healing processes. Several studies, conducted mainly in the orthopedic field, support the use of leukocyte-poor (LP) PRP, whereas other studies have not identified any significant differences between the use of LP and leukocyte-rich PRP. In the present study, the role of leukocytes contained in PRP was assessed to verify their in vitro effect on fibroblasts and endothelial cells, which have a leading role in the biological processes associated with wound healing (including angiogenesis and matrix remodeling)...
April 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29555206/effects-of-anthocyanins-and-their-gut-metabolites-on-adenosine-diphosphate-induced-platelet-activation-and-their-aggregation-with-monocytes-and-neutrophils
#15
Irena Krga, Nevena Vidovic, Dragan Milenkovic, Aleksandra Konic-Ristic, Filip Stojanovic, Christine Morand, Marija Glibetic
Accumulating evidence suggests that anthocyanins play an important role in the cardioprotective effects associated with consumption of anthocyanin-rich foods. These benefits may partly be attributed to their effects on platelets, significant contributors to cardiovascular disease development. This study aimed to investigate the impact of physiologically relevant concentrations of anthocyanins and their metabolites on platelet activation and platelet-leukocyte aggregation. Whole blood from seven healthy volunteers was treated with anthocyanins: cyanidin-3-arabinoside, cyanidin-3-glucoside, cyanidin-3-galactoside, delphinidin-3-glucoside and peonidin-3-glucoside at 0...
March 16, 2018: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29554150/effect-of-leukocyte-reduced-platelet-rich-plasma-on-osteoarthritis-caused-by-cranial-cruciate-ligament-rupture-a-canine-gait-analysis-model
#16
José M Vilar, Maria E Manera, Angelo Santana, Giuseppe Spinella, Oliver Rodriguez, Mónica Rubio, José M Carrillo, Joaquín Sopena, Miguel Batista
The goal of this study was to objectively assess the effect of a platelet-rich plasma (PRP) derivate in English bulldogs with stifle degenerative joint disease secondary to cranial cruciate ligament rupture (CCLR). We used a force platform and affixed electrogoniometers to measure peak vertical force (PVF), vertical impulse (VI), stance time (ST), and angular range of motion (AROM), from 12 lame client-owned English bulldogs with post-CCLR stifle joint abnormalities. The 12 affected subjects were treated with 4 intra-articular injections of PRP, at 30-day intervals...
2018: PloS One
https://www.readbyqxmd.com/read/29544939/circulating-microparticles-are-associated-with-clinical-severity-of-persistent-st-segment-elevation-myocardial-infarction-complicated-with-cardiogenic-shock
#17
A Sionis, R Suades, J Sans-Roselló, M Sánchez-Martínez, J Crespo, T Padró, J Cubedo, A Ferrero-Gregori, M Vila-Perales, A Duran-Cambra, L Badimon
BACKGROUND: Cardiogenic shock (CS) is the leading cause of death in patients admitted for acute myocardial infarction (MI). Despite the recent advances in reperfusion and medical treatment mortality remains unacceptably high. Whether cells of the blood compartment in CS-patients are activated and release microparticles (cMPs) that may be both messengers and biomarkers of cell damage is not known. We aimed to investigate the cMP subtypes and parental activated cells of ST-elevation MI (STEMI)-patients complicated by CS and that of non-CS STEMI-patients (non-CS) in order to identify a cMP signature that could aid CS patient's risk stratification...
May 1, 2018: International Journal of Cardiology
https://www.readbyqxmd.com/read/29534999/diverse-signalling-of-the-platelet-p2y-1-receptor-leads-to-a-dichotomy-in-platelet-function
#18
Richard T Amison, Shirin Jamshidi, Khondaker M Rahman, Clive P Page, Simon C Pitchford
Platelet P2Y1 receptor signalling via RhoGTPases is necessary for platelet-dependent leukocyte recruitment, where no platelet aggregation is observed. We investigated signalling cascades involved in distinct P2Y1 -dependent platelet activities in vitro, using specific inhibitors for phospholipase C (PLC) (U73122, to inhibit the canonical pathway), and RhoGTPases: Rac1 (NSC23766) and RhoA (ROCK inhibitor GSK429286). Human platelet rich plasma (for platelet aggregation) or isolated washed platelets (for chemotaxis assays) was treated with U73122, GSK429286 or NSC23766 prior to stimulation with adenosine diphosphate (ADP) or the P2Y1 specific agonist MRS2365...
May 15, 2018: European Journal of Pharmacology
https://www.readbyqxmd.com/read/29534512/anthocyanin-rich-extract-from-red-chinese-cabbage-alleviates-vascular-inflammation-in-endothelial-cells-and-apo-e-mice
#19
Hee Kyoung Joo, Sunga Choi, Yu Ran Lee, Eun Ok Lee, Myoung Soo Park, Kyu Been Park, Cuk-Seong Kim, Yong Pyo Lim, Jong-Tae Park, Byeong Hwa Jeon
Anthocyanins, the most prevalent flavonoids in red/purple fruits and vegetables, are known to improve immune responses and reduce chronic disease risks. In this study, the anti-inflammatory activities of an anthocyanin-rich extract from red Chinese cabbage (ArCC) were shown based on its inhibitory effects in cultured endothelial cells and hyperlipidemic apolipoprotein E -deficient mice. ArCC treatment suppressed monocyte adhesion to tumor necrosis factor-α-stimulated endothelial cells. This was validated by ArCC's ability to downregulate the expression and transcription of endothelial adhesion molecules, determined by immunoblot and luciferase promoter assays, respectively...
March 12, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29524025/root-end-surgery-with-leucocyte-and-platelet-rich-fibrin-and-an-occlusive-membrane-a-randomized-controlled-clinical-trial-on-patients-quality-of-life
#20
Nastaran Meschi, Steffen Fieuws, Anke Vanhoenacker, Olaf Strijbos, Dominique Van der Veken, Constantinus Politis, Paul Lambrechts
OBJECTIVES: The aim of this study was to investigate the impact of the adjunct of leukocyte- and platelet-rich fibrin (LPRF) to root-end surgery (RES) on the patients' quality of life during the first week post RES. MATERIALS AND METHODS: Patients in need of RES were recruited for an open randomized controlled clinical trial (RCT) with a 2 × 2 factorial design. They were randomly allocated to the test (+LPRF) and control (-LPRF) group. Each group was subsequently divided into two strata: with or without an occlusive membrane (Bio-Gide®, Geistlich Biomaterials, Switzerland; BG)...
March 9, 2018: Clinical Oral Investigations
keyword
keyword
17931
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"