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https://www.readbyqxmd.com/read/28437133/the-impact-of-the-centrifuge-characteristics-and-centrifugation-protocols-on-the-cells-growth-factors-and-fibrin-architecture-of-a-leukocyte-and-platelet-rich-fibrin-l-prf-clot-and-membrane
#1
David M Dohan Ehrenfest, Nelson R Pinto, Andrea Pereda, Paula Jiménez, Marco Del Corso, Byung-Soo Kang, Mauricio Nally, Nicole Lanata, Hom-Lay Wang, Marc Quirynen
L-PRF (leukocyte- and platelet-rich fibrin) is one of the four families of platelet concentrates for surgical use and is widely used in oral and maxillofacial regenerative therapies. The first objective of this article was to evaluate the mechanical vibrations appearing during centrifugation in four models of commercially available table-top centrifuges used to produce L-PRF and the impact of the centrifuge characteristics on the cell and fibrin architecture of a L-PRF clot and membrane. The second objective of this article was to evaluate how changing some parameters of the L-PRF protocol may influence its biological signature, independently from the characteristics of the centrifuge...
April 24, 2017: Platelets
https://www.readbyqxmd.com/read/28424777/influence-of-cellular-composition-and-exogenous-activation-on-growth-factor-and-cytokine-concentrations-in-canine-platelet-rich-plasmas
#2
Samuel P Franklin, Kate E Birdwhistell, Alena Strelchik, Bridget C Garner, Benjamin M Brainard
OBJECTIVE: The purposes of this study were to (1) evaluate correlations among platelet, leukocyte, growth factor, and cytokine concentrations in canine platelet-rich plasmas (PRPs) produced from five different canine PRP-concentrating systems and (2) compare the effects of different activation protocols on platelet activation and growth factor release from one of these PRPs. METHODS: PRP was made using blood from 15 dogs and each of 5 different PRP systems in a cross-over design...
2017: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/28401929/the-effect-of-l-prf-membranes-on-bone-healing-in-rabbit-tibiae-bone-defects-micro-ct-and-biomarker-results
#3
Fernanda Faot, Sanne Deprez, Katleen Vandamme, Germana V Camargos, Nelson Pinto, Jasper Wouters, Joost van den Oord, Marc Quirynen, Joke Duyck
More insight into the biological fundamentals of leukocyte platelet-rich fibrin (L-PRF) guided healing is necessary to recommend its application, in particular in deficient bone sites that need to support implants. This study investigated the short-term bone healing effect of L-PRF treatment in cylindrical non-critical sized bone defects with 3 mm diameter and 6 mm depth in tibiae of 18 adult male New Zealand White rabbits. After a randomization process, 96 bone defects were prepared and half of them were filled with a L-PRF membrane, while untreated defects in the opposite tibia served as control group...
April 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28375975/autologous-pure-platelet-rich-plasma-dermal-injections-for-facial-skin-rejuvenation-clinical-instrumental-and-flow-cytometry-assessment
#4
Norma Cameli, Maria Mariano, Iole Cordone, Elva Abril, Serena Masi, Maria Laura Foddai
BACKGROUND: Platelet-rich plasma (PRP) is an emerging treatment in dermatology recently proposed for skin rejuvenation. OBJECTIVE: To evaluate the efficacy and safety of autologous pure PRP dermal injections on facial skin rejuvenation, investigating the cellularity of PRP samples. MATERIALS AND METHODS: Twelve patients underwent 3 sessions of PRP injection at 1-month intervals. The clinical and instrumental outcomes were evaluated before (T0) and 1 month (T1) after the end of treatment by means of transepidermal water loss, corneometry, Cutometer, Visioscan, and Visioface...
April 3, 2017: Dermatologic Surgery: Official Publication for American Society for Dermatologic Surgery [et Al.]
https://www.readbyqxmd.com/read/28367492/analgesic-anti-inflammatory-and-antioxidant-activities-of-byrsonima-duckeana-w-r-anderson-malpighiaceae
#5
Maria Christina Dos Santos Verdam, Fernanda Guilhon-Simplicio, Kleyton Cardoso de Andrade, Karina Lorena Meira Fernandes, Tallita Marques Machado, Felipe Moura Araújo da Silva, Mayane Pereira de Souza, Hector Henrique Ferreira Koolen, Cristiane da Silva Paula, Beatriz Cristina Konopatzki Hirota, Vinícius Bednarczuk de Oliveira, Cristina Mayumi Sasaki Miyazaki, Milena Kalegari, Marilis Dallarmi Miguel, Patricia Maria Stuelp-Campelo, Obdulio Gomes Miguel
Background. Byrsonima is a promising neotropical genus, rich in flavonoids and triterpenes, with several proven pharmacological properties. Nevertheless, Byrsonima duckeana W. R. Anderson is an Amazonian species almost not studied. Objective. To assess the antioxidant, anti-inflammatory, and analgesic activities of Byrsonima duckeana leaves. Materials and Methods. We analyzed an ethanol extract and its fractions for polyphenol content and UHPLC-MS/MS, phosphomolybdenum, DPPH, TBARS antioxidant tests, formalin-induced pain, carrageenan-induced peritonitis, acetic acid-induced abdominal writhings, and hot plate assays...
2017: TheScientificWorldJournal
https://www.readbyqxmd.com/read/28364043/unraveling-the-structural-basis-for-the-unusually-rich-association-of-human-leukocyte-antigen-dq2-5-with-class-ii-associated-invariant-chain-peptides
#6
Thanh-Binh Nguyen, Priya Jayaraman, Elin Bergseng, Mallur S Madhusudhan, Chu-Young Kim, Ludvig M Sollid
Human leukocyte antigen (HLA)-DQ2.5 (DQA1*05/DQB1*02) is a class II major histocompatibility complex protein associated with both type 1 diabetes and celiac disease. One unusual feature of DQ2.5 is its high class-II-associated invariant chain peptide (CLIP) content. Moreover, DQ2.5 preferentially binds the non-canonical CLIP2 over the canonical CLIP1. To better understand the structural basis of DQ2.5's unusual CLIP association characteristics, better insight into the DQ2.5-CLIP complex structures is required...
March 31, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28352654/net-silencing-by-let-7i-in-postural-tachycardia-syndrome
#7
Abdul Waheed Khan, Mark Ziemann, Susan J Corcoran, Harikrishnan K N, Jun Okabe, Haloom Rafehi, Scott S Maxwell, Murray D Esler, Assam El-Osta
While strongly implicated in postural tachycardia syndrome (POTS), considerable controversy exists regarding norepinephrine transporter (NET) loss of function. POTS is characterized by the clinical symptoms of orthostatic intolerance, lightheadedness, tachycardia, and syncope or near syncope with upright posture. Abnormal sympathetic nervous system activity is typical, of a type which suggests dysfunction of the NET, with evidence that the gene responsible is under tight epigenetic control. Using RNA of isolated chromatin combined with massive parallel sequencing (RICh-seq) we show that let-7i miRNA suppresses NET by methyl-CpG-binding protein 2 (MeCP2)...
March 23, 2017: JCI Insight
https://www.readbyqxmd.com/read/28351189/effects-of-an-injectable-platelet-rich-fibrin-on-osteoblast-behavior-and-bone-tissue-formation-in-comparison-to-platelet-rich-plasma
#8
Xuzhu Wang, Yufeng Zhang, Joseph Choukroun, Shahram Ghanaati, Richard J Miron
Platelet-rich plasma (PRP) has been utilized for many years as a regenerative agent capable of inducing vascularization of various tissues using blood-derived growth factors. Despite this, drawbacks mostly related to the additional use of anti-coagulants found in PRP have been shown to inhibit the wound healing process. For these reasons, a novel platelet concentrate has recently been developed with no additives by utilizing lower centrifugation speeds. The purpose of this study was therefore to investigate osteoblast behavior of this novel therapy (injectable-platelet-rich fibrin; i-PRF, 100% natural with no additives) when compared to traditional PRP...
March 29, 2017: Platelets
https://www.readbyqxmd.com/read/28349485/monitoring-migration-of-activated-t-cells-to-antigen-rich-non-lymphoid-tissue
#9
Eleanor Jayne Ward, Hongmei Fu, Federica Marelli-Berg
Effective immunity requires appropriate recirculation of naïve T cells through secondary lymphoid organs and migration of antigen-specific T cells to sites of inflammation. Leukocyte migration is a highly regulated process requiring specific interactions between leukocytes and endothelial cells (EC) termed collectively as the leukocyte adhesion cascade. Recruitment and retention of activated T cells to antigen-rich sites of inflammation is a key event in the immune response, which relies in part on local antigen presentation particularly by EC of inflamed vessels...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28341267/in-vivo-clinical-and-radiological-effects-of-platelet-rich-plasma-on-interstitial-supraspinatus-lesion-case-series
#10
A Lädermann, M A Zumstein, F C Kolo, M Grosclaude, L Koglin, A J P Schwitzguebel
BACKGROUND: Rotator cuff tear (RCT) is a frequent condition of clinical relevance that can be managed with a symptomatic conservative treatment, but surgery is often needed. Biological components like leukocytes and platelet rich plasma (L-PRP) could represent an alternative curative method for interstitial RCT. HYPOTHESES: It has been hypothesized that an ultrasound guided L-PRP injection in supraspinatus interstitial RCT could induce radiological healing. MATERIAL AND METHODS: A prospective case series including 25 patients was performed in order to assess the effect of L-PRP infiltration into supraspinatus interstitial RCTs...
December 2016: Orthopaedics & Traumatology, Surgery & Research: OTSR
https://www.readbyqxmd.com/read/28339078/comparative-evaluation-of-the-effects-of-platelet%C3%A2-rich-plasma-formulations-on-extracellular-matrix-formation-and-the-nf%C3%A2-%C3%AE%C2%BAb-signaling-pathway-in-human-articular-chondrocytes
#11
Wenjing Yin, Haitao Xu, Jiagen Sheng, Zhengliang Xu, Xuetao Xie, Changqing Zhang
Concentrated leukocytes in leukocyte and platelet‑rich plasma (L‑PRP) may deliver increased levels of pro‑inflammatory cytokines to activate the nuclear factor (NF)‑κB signaling pathway, to counter or overwhelm the beneficial effects of growth factors on cartilage regeneration. However, to date, no relevant studies have substantiated this. In the present study, L‑PRP and pure platelet‑rich plasma (P‑PRP) were prepared, and leukocytes, platelets, pro‑inflammatory cytokines and growth factor concentrations were quantified; they were then used to treat human articular chondrocytes (HACs)...
May 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28329021/comparison-of-anti-inflammatory-activities-of-an-anthocyanin-rich-fraction-from-portuguese-blueberries-vaccinium-corymbosum-l-and-5-aminosalicylic-acid-in-a-tnbs-induced-colitis-rat-model
#12
Sónia R Pereira, Rita Pereira, Isabel Figueiredo, Victor Freitas, Teresa C P Dinis, Leonor M Almeida
Despite the actual therapeutic approaches for inflammatory bowel disease (IBD), efficient and secure alternative options remain a research focus. In this context, anthocyanins seem promising natural anti-inflammatory agents, but their action mechanisms and efficacy as compared with established drugs still require more clarification. The main aim of this study was to compare the anti-inflammatory action of a chemically characterized anthocyanin-rich fraction (ARF), obtained from Portuguese blueberries (Vaccinium corymbosum L...
2017: PloS One
https://www.readbyqxmd.com/read/28324162/reduction-of-relative-centrifugal-forces-increases-growth-factor-release-within-solid-platelet-rich-fibrin-prf-based-matrices-a-proof-of-concept-of-lscc-low-speed-centrifugation-concept
#13
K El Bagdadi, A Kubesch, X Yu, S Al-Maawi, A Orlowska, A Dias, P Booms, E Dohle, R Sader, C J Kirkpatrick, J Choukroun, S Ghanaati
Purpose The present study evaluated the platelet distribution pattern and growth factor release (VEGF, TGF-β1 and EGF) within three PRF (platelet-rich-fibrin) matrices (PRF, A-PRF and A-PRF+) that were prepared using different relative centrifugation forces (RCF) and centrifugation times. Materials and methods immunohistochemistry was conducted to assess the platelet distribution pattern within three PRF matrices. The growth factor release was measured over 10 days using ELISA. Results The VEGF protein content showed the highest release on day 7; A-PRF+ showed a significantly higher rate than A-PRF and PRF...
March 21, 2017: European Journal of Trauma and Emergency Surgery: Official Publication of the European Trauma Society
https://www.readbyqxmd.com/read/28302625/silencing-of-anticoagulant-protein-c-evokes-low-incident-but-spontaneous-atherothrombosis-in-apolipoprotein-e-deficient-mice
#14
Amber B Ouweneel, Marco Heestermans, Robin A F Verwilligen, Marion J J Gijbels, Pieter H Reitsma, Miranda van Eck, Bart J M van Vlijmen
OBJECTIVE: Murine atherosclerosis models do not spontaneously develop atherothrombotic complications. We investigated whether disruption of natural anticoagulation allows preexisting atherosclerotic plaques to progress toward an atherothrombotic phenotype. APPROACH AND RESULTS: On lowering of plasma protein C levels with small interfering RNA (siProc) in 8-week Western-type diet-fed atherosclerotic apolipoprotein E-deficient mice, one out of 4 mice displayed a large, organized, and fibrin- and leukocyte-rich thrombus on top of an advanced atherosclerotic plaque located in the aortic root...
March 16, 2017: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/28301205/intratendon-delivery-of-leukocyte-poor-platelet-rich-plasma-improves-healing-compared-with-leukocyte-rich-platelet-rich-plasma-in-a-rabbit-achilles-tendinopathy-model
#15
Ruijian Yan, Yanjia Gu, Jisheng Ran, Yejun Hu, Zefeng Zheng, Mengfeng Zeng, Boon Chin Heng, Xiao Chen, Zi Yin, Weishan Chen, Weiliang Shen, Hongwei Ouyang
BACKGROUND: Chronic tendinopathy is a commonly occurring clinical problem that affects both athletes and inactive middle-aged patients. Although some studies have shown that different platelet-rich plasma (PRP) preparations could exert various therapeutic effects in vitro, the role of leukocytes in PRP has not yet been defined under tendinopathy conditions in vivo. PURPOSE: This study compared the effects of the intratendon delivery of leukocyte-poor PRP (Lp-PRP) versus leukocyte-rich PRP (Lr-PRP) in a rabbit chronic tendinopathy model in vivo...
March 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/28296518/effect-of-platelet-rich-plasma-on-rat-achilles-tendon-healing-is-related-to-microbiota
#16
Franciele Dietrich, Malin Hammerman, Parmis Blomgran, Love Tätting, Vinicius Faccin Bampi, Jefferson Braga Silva, Per Aspenberg
Background and purpose - In 3 papers in Acta Orthopaedica 10 years ago, we described that platelet-rich plasma (PRP) improves tendon healing in a rat Achilles transection model. Later, we found that microtrauma has similar effects, probably acting via inflammation. This raised the suspicion that the effect ascribed to growth factors within PRP could instead be due to unspecific influences on inflammation. While testing this hypothesis, we noted that the effect seemed to be related to the microbiota. Material and methods - We tried to reproduce our old findings with local injection of PRP 6 h after tendon transection, followed by mechanical testing after 11 days...
March 15, 2017: Acta Orthopaedica
https://www.readbyqxmd.com/read/28283682/reduction-of-relative-centrifugation-force-within-injectable-platelet-rich-fibrin-prf-concentrates-advances-patients-own-inflammatory-cells-platelets-and-growth-factors-the-first-introduction-to-the-low-speed-centrifugation-concept
#17
J Choukroun, S Ghanaati
PURPOSE: The aim of this study was to analyze systematically the influence of the relative centrifugation force (RCF) on leukocytes, platelets and growth factor release within fluid platelet-rich fibrin matrices (PRF). MATERIALS AND METHODS: Systematically using peripheral blood from six healthy volunteers, the RCF was reduced four times for each of the three experimental protocols (I-III) within the spectrum (710-44 g), while maintaining a constant centrifugation time...
March 10, 2017: European Journal of Trauma and Emergency Surgery: Official Publication of the European Trauma Society
https://www.readbyqxmd.com/read/28265109/comparative-evaluation-of-leukocyte-and-platelet-rich-plasma-and-pure-platelet-rich-plasma-for-cartilage-regeneration
#18
Zhengliang Xu, Wenjing Yin, Yuelei Zhang, Xin Qi, Yixuan Chen, Xuetao Xie, Changqing Zhang
Platelet-rich plasma (PRP) has gained growing popularity in the treatment of articular cartilage lesions in the last decade. However, the potential harmful effects of leukocytes in PRP on cartilage regeneration have seldom been studied in vitro, and not at all in vivo yet. The objective of the present study is to compare the effects of leukocyte- and platelet-rich plasma (L-PRP) and pure platelet-rich plasma (P-PRP) on cartilage repair and NF-κB pathway, in order to explore the mechanism underlying the function of leukocytes in PRP in cartilage regeneration...
March 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28255569/platelet-rich-plasma-injections-for-advanced-knee-osteoarthritis-a-prospective-randomized-double-blinded-clinical-trial
#19
Nayana Joshi Jubert, Luciano Rodríguez, Maria Mercedes Reverté-Vinaixa, Aurora Navarro
BACKGROUND: Intra-articular injections of platelet-rich plasma (PRP) to treat symptoms of knee osteoarthritis (OA) have been successfully used in young patients and in the early stages of disease. No previous studies have analyzed outcomes of PRP injections during the late stages. HYPOTHESIS: PRP reduces pain and leads to a more effective and lasting functional recovery than corticosteroid with local anesthetic. STUDY DESIGN: Randomized controlled trial; Level of evidence, 2...
February 2017: Orthopaedic Journal of Sports Medicine
https://www.readbyqxmd.com/read/28248701/autoimmune-encephalitis-with-anti-leucine-rich-glioma-inactivated-1-or-anti-contactin-associated-protein-like-2-antibodies-formerly-called-voltage-gated-potassium-channel-complex-antibodies
#20
Anna E M Bastiaansen, Agnes van Sonderen, Maarten J Titulaer
PURPOSE OF REVIEW: Twenty years since the discovery of voltage-gated potassium channel (VGKC)-related autoimmunity; it is currently known that the antibodies are not directed at the VGKC itself but to two closely associated proteins, anti-leucine-rich glioma-inactivated 1 (LGI1) and contactin-associated protein-like 2 (Caspr2). Antibodies to LGI1 and Caspr2 give well-described clinical phenotypes. Anti-LGI1 encephalitis patients mostly have limbic symptoms, and anti-Caspr2 patients have variable syndromes with both central and peripheral symptoms...
June 2017: Current Opinion in Neurology
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