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Induced pluripotent stem cells

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https://www.readbyqxmd.com/read/29667777/induced-pluripotent-stem-cell-derived-conditional-medium-promotes-leydig-cell-anti-apoptosis-and-proliferation-via-autophagy-and-wnt-%C3%AE-catenin-pathway
#1
Xiaoling Guo, Yong Chen, Tingting Hong, Xianwu Chen, Yue Duan, Chao Li, Renshan Ge
Leydig cell transplantation is a better alternative in the treatment of androgen-deficient males. The main purpose of this study was to investigate the effects of induced pluripotent stem cell-derived conditioned medium (iPS-CM) on the anti-apoptosis, proliferation and function of immature Leydig cells (ILCs), and illuminate the underlying mechanisms. ILCs were exposed to 200 μmol/L hydrogen peroxide (H2 O2 ) for 24 hours with or without iPS-CM treatments. Cell apoptosis was detected by flow cytometric analysis...
April 18, 2018: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/29666931/genome-editing-and-induced-pluripotent-stem-cell-technologies-for-personalized-study-of-cardiovascular-diseases
#2
REVIEW
Young Wook Chun, Matthew D Durbin, Charles C Hong
PURPOSE OF REVIEW: The goal of this review is to highlight the potential of induced pluripotent stem cell (iPSC)-based modeling as a tool for studying human cardiovascular diseases. We present some of the current cardiovascular disease models utilizing genome editing and patient-derived iPSCs. RECENT FINDINGS: The incorporation of genome-editing and iPSC technologies provides an innovative research platform, providing novel insight into human cardiovascular disease at molecular, cellular, and functional level...
April 17, 2018: Current Cardiology Reports
https://www.readbyqxmd.com/read/29666786/modeling-neuropsychiatric-and-neurodegenerative-diseases-with-induced-pluripotent-stem-cells
#3
REVIEW
Elizabeth A LaMarca, Samuel K Powell, Schahram Akbarian, Kristen J Brennand
Human-induced pluripotent stem cells (hiPSCs) have revolutionized our ability to model neuropsychiatric and neurodegenerative diseases, and recent progress in the field is paving the way for improved therapeutics. In this review, we discuss major advances in generating hiPSC-derived neural cells and cutting-edge techniques that are transforming hiPSC technology, such as three-dimensional "mini-brains" and clustered, regularly interspersed short palindromic repeats (CRISPR)-Cas systems. We examine specific examples of how hiPSC-derived neural cells are being used to uncover the pathophysiology of schizophrenia and Parkinson's disease, and consider the future of this groundbreaking research...
2018: Frontiers in Pediatrics
https://www.readbyqxmd.com/read/29663886/microrna-regulation-along-the-course-of-cellular-reprogramming-to-pluripotency
#4
M Ishtiaq, M Waseem, R Mehmood
Generation of patient-specific stem cells has been a long-held aim of many developmental biologists. Apart from providing a source for stem cell therapies, these cells have the potential to be utilized in a number of scenarios like disease modeling, drug screening and studying normal development. Various approaches have been used to reprogram terminally differentiated cells to a pluripotent state with varying efficiencies and limitations. The nuclear transfer had been the most successful method for reprogramming until recently...
April 15, 2018: Current Molecular Medicine
https://www.readbyqxmd.com/read/29663322/quantitatively-characterizing-drug-induced-arrhythmic-contractile-motions-of-human-stem-cell-derived-cardiomyocytes
#5
Plansky Hoang, Nathaniel Huebsch, Shin Hyuk Bang, Brian A Siemons, Bruce R Conklin, Kevin E Healy, Zhen Ma, Sabir Jacquir
Quantification of abnormal contractile motions of cardiac tissue has been a noteworthy challenge and significant limitation in assessing and classifying the drug-induced arrhythmias (i.e. Torsades de pointes). To overcome these challenges, researchers have taken advantage of computational image processing tools to measure contractile motion from cardiomyocytes derived from human induced pluripotent stem cells (hiPSC-CMs). However, the amplitude and frequency analysis of contractile motion waveforms doesn't produce sufficient information to objectively classify the degree of variations between two or more sets of cardiac contractile motions...
April 16, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29661985/a-net-shaped-multicellular-formation-facilitates-the-maturation-of-hpsc-derived-cardiomyocytes-through-mechanical-and-electrophysiological-stimuli
#6
Taoyan Liu, Chengwu Huang, Hongxia Li, Fujian Wu, Jianwen Luo, Wenjing Lu, Feng Lan
The use of human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) is limited in drug discovery and cardiac disease mechanism studies due to cell immaturity. Although many approaches have been reported to improve the maturation of hiPSC-CMs, the elucidation of the process of maturation is crucial. We applied a small-molecule-based differentiation method to generate cardiomyocytes (CMs) with multiple aggregation forms. The motion analysis revealed significant physical differences in the differently shaped CMs, and the net-shaped CMs had larger motion amplitudes and faster velocities than the sheet-shaped CMs...
April 14, 2018: Aging
https://www.readbyqxmd.com/read/29661116/crispr-cas9-targeted-deletion-of-polyglutamine-in-spinocerebellar-ataxia-type-3-derived-induced-pluripotent-stem-cells
#7
Shuming Ouyang, Yingjun Xie, Zeyu Xiong, Yi Yang, Yexing Xian, Zhanhui Ou, Bing Song, Yuchang Chen, Yuhuan Xie, Haoxian Li, Xiaofang Sun
Spinocerebellar ataxia type 3 (SCA3) is caused by an abnormal expansion of the CAG triplet in ATXN3, which translates into a polyglutamine (polyQ) tract within ataxin-3 (ATXN3) protein. Although the pathogenic mechanisms remain unclear, it is well established that expression of mutant forms of ATXN3 carrying an expanded polyQ domain are involved in SCA3 pathogenesis, and several strategies to suppress mutant ATXN3 have showed promising potential for SCA3 treatment. In this study, we described successful CRISPR/Cas9-mediated deletion of the expanded polyQ-encoding region of ATXN3 in induced pluripotent stem cells (iPSCs) derived from a SCA3 patient, and these patient-specific iPSCs retained pluripotency and neural differentiation following expanded polyQ deletion...
April 17, 2018: Stem Cells and Development
https://www.readbyqxmd.com/read/29660608/generation-and-characterization-of-two-human-ipsc-lines-from-patients-with-methylmalonic-acidemia-cblb-type
#8
E Richard, S Brasil, A Briso-Montiano, E Alonso-Barroso, M E Gallardo, B Merinero, M Ugarte, L R Desviat, B Pérez
Two human induced pluripotent stem cell (iPSC) lines were generated from fibroblasts of two siblings with methylmalonic acidemia cblB type carrying mutations in the MMAB gene: c.287T➔C (p.Ile96Thr) and a splicing loss-of-function variant c.584G➔A affecting the last nucleotide of exon 7 in MMAB (p.Ser174Cysfs*23). Reprogramming factors OCT3/4, SOX2, KLF4 and c-MYC were delivered using a non-integrative method based on the Sendai virus. Once established, iPSCs have shown full pluripotency, differentiation capacity and genetic stability...
April 5, 2018: Stem Cell Research
https://www.readbyqxmd.com/read/29660607/generation-of-an-ips-cell-line-via-a-non-integrative-method-using-urine-derived-cells-from-a-patient-with-ush2a-associated-retinitis-pigmentosa
#9
Yonglong Guo, Qiaolang Zeng, Shiwei Liu, Quan Yu, Peiyuan Wang, Hongjie Ma, Shanshan Shi, Xin Yan, Zekai Cui, Mengyuan Xie, Yunxia Xue, Qingbing Zha, Zhijie Li, Jun Zhang, Shibo Tang, Jiansu Chen
We have established an induced pluripotent stem (iPS) cell line using urine-derived cells from a 27-year-old male patient with retinitis pigmentosa associated with point mutations in the USH2A gene. Feeder-free culture conditions and the integration-free CytoTune™-iPS 2.0 Sendai Reprogramming Kit were used.
April 6, 2018: Stem Cell Research
https://www.readbyqxmd.com/read/29660606/generation-of-induced-pluripotent-stem-cells-from-a-patient-with-best-dystrophy-carrying-11q12-3-best1-vmd2-mutation
#10
Chih-Chien Hsu, Huai-En Lu, Jen-Hua Chuang, Yu-Ling Ko, Yi-Ching Tsai, Hsiao-Yun Tai, Aliaksandr A Yarmishyn, De-Kuang Hwang, Mong-Lien Wang, Yi-Ping Yang, Shih-Jen Chen, Chi-Hsien Peng, Shih-Hwa Chiou, Tai-Chi Lin
Best disease (BD), also termed Best vitelliform macular dystrophy (BVMD), is a juvenile-onset form of macular degeneration and central visual loss. In this report, we generated an induced pluripotent stem cell (iPSC) line, TVGH-iPSC-012-04, from the peripheral blood mononuclear cells of a female patient with BD by using the Sendai virus delivery system. The resulting iPSCs retained the disease-causing DNA mutation, expressed pluripotent markers and could differentiate into three germ layers. We believe that BD patient-specific iPSCs provide a powerful in vitro model for evaluating the pathological phenotypes of the disease...
April 3, 2018: Stem Cell Research
https://www.readbyqxmd.com/read/29660533/role-of-lysophosphatidic-acid-in-the-retinal-pigment-epithelium-and-photoreceptors
#11
Grace E Lidgerwood, Andrew J Morris, Alison Conquest, Maciej Daniszewski, Louise A Rooney, Shiang Y Lim, Damián Hernández, Helena H Liang, Penelope Allen, Paul P Connell, Robyn H Guymer, Alex W Hewitt, Alice Pébay
The human retina is a complex structure of organised layers of specialised cells that support the transmission of light signals to the visual cortex. The outermost layer of the retina, the retinal pigment epithelium (RPE) forms part of the blood retina barrier and is implicated in many retinal diseases. Lysophosphatidic acid (LPA) is a bioactive lipid exerting pleiotropic effects in various cell types, during development, normal physiology and disease. Its producing enzyme AUTOTAXIN (ATX) is highly expressed by the pigmented epithelia of the human eye, including the RPE...
April 13, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29658608/ipsc%C3%A2-msc-inhibition-assessment-in-raw-264-7-cells-following-oxygen-and-glucose-deprivation-reveals-a-distinct-function-for-cardiopulmonary-resuscitation
#12
Yi Yu, Dongping Wang, Hui Li, Yujie Liu, Zhao Xiang, Junlin Wu, Xiaoli Jing
Hypoxia is a serious stress state. The nervous system is less tolerant to hypoxia, and cell death due to hypoxia is irreversible. With the incidence of cardiovascular disease gradually increasing, the sudden cardiac death rate is additionally increasing. Although cardiopulmonary resuscitation (CPR) is an important development, recovery is frequently poor. In a successful recovery population, ~40% of the population was in a vegetative state or subsequently succumbed to their condition, and ~20% had brain damage...
April 11, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29658075/induced-pluripotent-stem-cell-derived-hematopoietic-embryoid-bodies-secrete-sphingosine-1-phosphate-and-revert-endothelial-injury
#13
S Kasuda, R Kudo, K Yuui, Y Sakurai, K Hatake
The possibility of sphingosine-1-phosphate production by induced pluripotent stem cells is examined to assess their potential in treatment of sepsis. The hematopoietic embryoid bodies were derived from the culture of 6-day-old differentiated induced pluripotent stem cells. These embryoid bodies secreted sphingosine-1-phosphate, an important bioactive lipid that regulates integrity of the pulmonary endothelial barrier, prevents elevation of its permeability, and impedes the formation of stress fibers in human endotheliocytes derived from umbilical vein...
April 16, 2018: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29657098/inflammatory-responses-and-barrier-function-of-endothelial-cells-derived-from-human-induced-pluripotent-stem-cells
#14
Oleh V Halaidych, Christian Freund, Francijna van den Hil, Daniela C F Salvatori, Mara Riminucci, Christine L Mummery, Valeria V Orlova
Several studies have reported endothelial cell (EC) derivation from human induced pluripotent stem cells (hiPSCs). However, few have explored their functional properties in depth with respect to line-to-line and batch-to-batch variability and how they relate to primary ECs. We therefore carried out accurate characterization of hiPSC-derived ECs (hiPSC-ECs) from multiple (non-integrating) hiPSC lines and compared them with primary ECs in various functional assays, which included barrier function using real-time impedance spectroscopy with an integrated assay of electric wound healing, endothelia-leukocyte interaction under physiological flow to mimic inflammation and angiogenic responses in in vitro and in vivo assays...
April 9, 2018: Stem Cell Reports
https://www.readbyqxmd.com/read/29656178/generation-of-3-spinocerebellar-ataxia-type-1-sca1-patient-derived-induced-pluripotent-stem-cell-lines-lumci002-a-b-and-c-and-2-unaffected-sibling-control-induced-pluripotent-stem-cell-lines-lumci003-a-and-b
#15
Ronald A M Buijsen, Sarah L Gardiner, Marga J Bouma, Linda M van der Graaf, Merel W Boogaard, Barry A Pepers, Bert Eussen, Annelies de Klein, Christian Freund, Willeke M C van Roon-Mom
Spinocerebellar ataxia type 1 (SCA1) is a hereditary neurodegenerative disease caused by a CAG repeat expansion in exon 8 of the ATXN1 gene. We generated induced pluripotent stem cells (hiPSCs) from a SCA1 patient and his non-affected sister by using non-integrating Sendai Viruses (SeV). The resulting hiPSCs are SeVfree, express pluripotency markers, display a normal karyotype, retain the mutation (length of the CAG repeat expansion in the ATXN1 gene) and are able to differentiate into the three germ layers in vitro...
April 5, 2018: Stem Cell Research
https://www.readbyqxmd.com/read/29653394/generation-of-duchenne-muscular-dystrophy-patient-specific-induced-pluripotent-stem-cell-line-lacking-exons-45-50-of-the-dystrophin-gene-iiti001-a
#16
Binyamin Eisen, Ronen Ben Jehuda, Ashley J Cuttitta, Lucy N Mekies, Irina Reiter, Sindhu Ramchandren, Michael Arad, Daniel E Michele, Ofer Binah
Duchenne muscular dystrophy (DMD) is an X-linked progressive muscle degenerative disease caused by mutations in the dystrophin gene. We generated induced pluripotent stem cells (iPSCs) from a 13-year-old male patient carrying a deletion mutation of exons 45-50; iPSCs were subsequently differentiated into cardiomyocytes. iPSCs exhibit expression of the pluripotent markers (SOX2, NANOG, OCT4), differentiation capacity into the three germ layers, normal karyotype, genetic identity to the skin biopsy dermal fibroblasts and the patient-specific dystrophin mutation...
April 3, 2018: Stem Cell Research
https://www.readbyqxmd.com/read/29653250/pluripotent-stem-cell-based-therapy-for-parkinson-s-disease-current-status-and-future-prospects
#17
REVIEW
Kai-C Sonntag, Bin Song, Nayeon Lee, Jin Hyuk Jung, Young Cha, Pierre Leblanc, Carolyn Neff, Sek Won Kong, Bob S Carter, Jeffrey Schweitzer, Kwang-Soo Kim
Parkinson's disease (PD) is one of the most common neurodegenerative disorders, which affects about 0.3% of the general population. As the population in the developed world ages, this creates an escalating burden on society both in economic terms and in quality of life for these patients and for the families that support them. Although currently available pharmacological or surgical treatments may significantly improve the quality of life of many patients with PD, these are symptomatic treatments that do not slow or stop the progressive course of the disease...
April 10, 2018: Progress in Neurobiology
https://www.readbyqxmd.com/read/29653124/doxorubicin-triggers-bioenergetic-failure-and-p53-activation-in-mouse-stem-cell-derived-cardiomyocytes
#18
Teresa Cunha-Oliveira, Luciana L Ferreira, Ana Raquel Coelho, Cláudia M Deus, Paulo J Oliveira
Doxorubicin (DOX) is a widely used anticancer drug that could be even more effective if its clinical dosage was not limited because of delayed cardiotoxicity. Beating stem cell-derived cardiomyocytes are a preferred in vitro model to further uncover the mechanisms of DOX-induced cardiotoxicity. Our objective was to use cultured induced-pluripotent stem cell(iPSC)-derived mouse cardiomyocytes (Cor.At) to investigate the effects of DOX on cell and mitochondrial metabolism, as well as on stress responses. Non-proliferating and beating Cor...
April 10, 2018: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29652595/stem-cells-for-skeletal-muscle-tissue-engineering
#19
Molly N Pantellic, Lisa Marie Larkin
Volumetric muscle loss (VML) is a debilitating condition wherein muscle loss overwhelms the body's normal physiological repair mechanism. VML is particularly common among military service members who have sustained war injuries. Because of the high social and medical cost associated with VML and suboptimal current surgical treatments, there is great interest in developing better VML therapies. Skeletal muscle tissue engineering (SMTE) is a promising alternative to traditional VML surgical treatments that use autogenic tissue grafts, and rather uses isolated stem cells with myogenic potential to generate de novo skeletal muscle tissues to treat VML...
March 14, 2018: Tissue Engineering. Part B, Reviews
https://www.readbyqxmd.com/read/29652170/pulmonary-transplantation-of-human-ipsc-derived-macrophages-ameliorates-pulmonary-alveolar-proteinosis
#20
Christine Happle, Nico Lachmann, Mania Ackermann, Anja Mirenska, Gudrun Göhring, Kathrin Thomay, Adele Mucci, Miriam Hetzel, Torsten Glomb, Takuji Suzuki, Claudia Chalk, Silke Glage, Oliver Dittrich-Breiholz, Bruce Trapnell, Thomas Moritz, Gesine Hansen
Rationale While the transplantation of induced pluripotent stem cell (iPSC)-derived cells harbors enormous potential for the treatment of pulmonary diseases, in vivo data demonstrating clear therapeutic benefits of human iPSC-derived cells in lung disease models is missing. Objective We here tested the therapeutic potential of iPSC-derived macrophages in a humanized disease model of hereditary pulmonary alveolar proteinosis (herPAP). herPAP is caused by a genetic defect of the GM-CSF-receptor, which leads to disturbed macrophage differentiation and protein/surfactant degradation in the lungs subsequently resulting in severe respiratory insufficiency...
April 13, 2018: American Journal of Respiratory and Critical Care Medicine
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